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Urhan E, Kara CS, Oguz EF, Neselioglu S, Erel O, Altuntas HD, Bayram F. The assessment of thiol-disulfide homeostasis and ıschemia-modified albumin levels in patients with acromegaly. Pituitary 2025; 28:46. [PMID: 40186831 PMCID: PMC11972178 DOI: 10.1007/s11102-025-01519-y] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Grants] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Submit a Manuscript] [Subscribe] [Scholar Register] [Accepted: 03/23/2025] [Indexed: 04/07/2025]
Abstract
PURPOSE Data regarding the relationship between acromegaly and oxidative stress (OS) remain limited. Dynamic thiol-disulfide homeostasis (TDH) is vital for antioxidant protection, and ischemia-modified albumin (IMA) serves as a marker of OS. This study aimed to measure serum TDH parameters and IMA levels in acromegaly patients, comparing them with healthy controls. METHODS This cross-sectional study consecutively included 81 patients and 55 controls, matched for age, gender, and body mass index. Serum levels of native thiol, total thiol, and disulfide (TDH parameters) were measured using the automated spectrophotometric method developed by Erel and Neselioglu, along with serum IMA levels. RESULTS In patients, serum native and total thiol levels were significantly lower (p = 0.005 and p = 0.007), while serum IMA levels were significantly higher (p = 0.001). Disulfide levels were similar. Patients with active disease (N = 32), patients in remission (N = 49), and controls (N = 55) were compared. In post-hoc analyses; serum TDH parameters and IMA levels were similar in remission and active disease patients. Native and total thiol levels were significantly lower in patients in remission compared to controls (p = 0.01 and p = 0.04). IMA levels were significantly higher in patients in remission compared to controls (p = 0.04). Serum thiol levels positively correlated with serum insulin-like growth factor-1 levels and negatively with age and disease duration, while age independently exerted a negative impact on serum thiol levels. CONCLUSION Our findings may indicate increased OS in the acromegalic process, which may contribute to the development of acromegaly and its related complications and comorbidities.
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Affiliation(s)
- Emre Urhan
- Department of Endocrinology, Erciyes University Medical School, Kayseri, Turkey.
| | - Canan Sehit Kara
- Department of Endocrinology, Erciyes University Medical School, Kayseri, Turkey
| | - Esra Fırat Oguz
- Department of Medical Biochemistry, Ankara City Hospital, Ankara, Turkey
| | - Salim Neselioglu
- Department of Medical Biochemistry, Yildirim Beyazit University Medical School, Ankara, Turkey
| | - Ozcan Erel
- Department of Medical Biochemistry, Yildirim Beyazit University Medical School, Ankara, Turkey
| | | | - Fahri Bayram
- Department of Endocrinology, Erciyes University Medical School, Kayseri, Turkey
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Li X, Ding F, Zhang L, Zhao S, Hu Z, Ma Z, Li F, Zhang Y, Zhao Y, Zhao Y. Interpretable machine learning method to predict the risk of pre-diabetes using a national-wide cross-sectional data: evidence from CHNS. BMC Public Health 2025; 25:1145. [PMID: 40140819 PMCID: PMC11938594 DOI: 10.1186/s12889-025-22419-7] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Grants] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 05/06/2024] [Accepted: 03/20/2025] [Indexed: 03/28/2025] Open
Abstract
OBJECTIVE The incidence of Type 2 Diabetes Mellitus (T2DM) continues to rise steadily, significantly impacting human health. Early prediction of pre-diabetic risks has emerged as a crucial public health concern in recent years. Machine learning methods have proven effective in enhancing prediction accuracy. However, existing approaches may lack interpretability regarding underlying mechanisms. Therefore, we aim to employ an interpretable machine learning approach utilizing nationwide cross-sectional data to predict pre-diabetic risk and quantify the impact of potential risks. METHODS The LASSO regression algorithm was used to conduct feature selection from 30 factors, ultimately identifying nine non-zero coefficient features associated with pre-diabetes, including age, TG, TC, BMI, Apolipoprotein B, TP, leukocyte count, HDL-C, and hypertension. Various machine learning algorithms, including Extreme Gradient Boosting (XGBoost), Random Forest (RF), Support Vector Machine (SVM), Naive Bayes (NB), Artificial Neural Networks (ANNs), Decision Trees (DT), and Logistic Regression (LR), were employed to compare predictive performance. Employing an interpretable machine learning approach, we aimed to enhance the accuracy of pre-diabetes risk prediction and quantify the impact and significance of potential risks on pre-diabetes. RESULTS From the China Health and Nutrition Survey (CHNS) data, a cohort of 8,277 individuals was selected, exhibiting a disease prevalence of 7.13%. The XGBoost model demonstrated superior performance with an AUC value of 0.939, surpassing RF, SVM, DT, ANNs, Naive Bayes, and LR models. Additionally, Shapley Additive Explanation (SHAP) analysis indicated that age, BMI, TC, ApoB, TG, hypertension, TP, HDL-C, and WBC may serve as risk factors for pre-diabetes. CONCLUSION The constructed model comprises nine easily accessible predictive factors, which prove highly effective in forecasting the risk of pre-diabetes. Concurrently, we have quantified the specific impact of each predictive factor on the risk and ranked them based on their influence. This result may serve as a convenient tool for early identification of individuals at high risk of pre-diabetes, providing effective guidance for preventing the progression of pre-diabetes to T2DM.
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Affiliation(s)
- Xiaolong Li
- School of Public Health, Ningxia Medical University, Yinchuan Ningxia, 750004, China
- NHC Key Laboratory of Metabolic Cardiovascular Diseases Research, Ningxia Medical University, Yinchuan, 750004, China
- Ningxia Key Laboratory of Environmental Factors and Chronic Disease Control, Yinchuan Ningxia, 750004, China
| | - Fan Ding
- School of Public Health, Ningxia Medical University, Yinchuan Ningxia, 750004, China
- NHC Key Laboratory of Metabolic Cardiovascular Diseases Research, Ningxia Medical University, Yinchuan, 750004, China
- Ningxia Key Laboratory of Environmental Factors and Chronic Disease Control, Yinchuan Ningxia, 750004, China
| | - Lu Zhang
- School of Public Health, Ningxia Medical University, Yinchuan Ningxia, 750004, China
- NHC Key Laboratory of Metabolic Cardiovascular Diseases Research, Ningxia Medical University, Yinchuan, 750004, China
- Ningxia Key Laboratory of Environmental Factors and Chronic Disease Control, Yinchuan Ningxia, 750004, China
| | - Shi Zhao
- School of Public Health, Tianjin Medical University, Tianjin, 300070, China
| | - Zengyun Hu
- School of Public Health, Shanghai Jiao Tong University, Shanghai, 200025, China
| | - Zhanbing Ma
- School of Basic Medicine, Ningxia Medical University, Yinchuan Ningxia, 750004, China
| | - Feng Li
- Department of Laboratory Medicine, General Hospital of Ningxia Medical University, Yinchuan Ningxia, 750004, China
| | - Yuhong Zhang
- School of Public Health, Ningxia Medical University, Yinchuan Ningxia, 750004, China
- NHC Key Laboratory of Metabolic Cardiovascular Diseases Research, Ningxia Medical University, Yinchuan, 750004, China
- Ningxia Key Laboratory of Environmental Factors and Chronic Disease Control, Yinchuan Ningxia, 750004, China
| | - Yi Zhao
- School of Public Health, Ningxia Medical University, Yinchuan Ningxia, 750004, China.
- NHC Key Laboratory of Metabolic Cardiovascular Diseases Research, Ningxia Medical University, Yinchuan, 750004, China.
- Ningxia Key Laboratory of Environmental Factors and Chronic Disease Control, Yinchuan Ningxia, 750004, China.
| | - Yu Zhao
- School of Public Health, Ningxia Medical University, Yinchuan Ningxia, 750004, China.
- NHC Key Laboratory of Metabolic Cardiovascular Diseases Research, Ningxia Medical University, Yinchuan, 750004, China.
- Ningxia Key Laboratory of Environmental Factors and Chronic Disease Control, Yinchuan Ningxia, 750004, China.
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Muduroglu A, Velioglu Y, Celik H, Yuksel A, Aldemir M, Atasoy MS, Kilic AO, Cetintas D, Badem S, Cayir MC, Gok G, Bal C. Evaluation of the dynamic thiol/disulfide homeostasis in patients with chronic venous insufficiency. J Vasc Surg Venous Lymphat Disord 2024; 12:101689. [PMID: 37788743 PMCID: PMC11523348 DOI: 10.1016/j.jvsv.2023.09.003] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 06/24/2023] [Revised: 09/15/2023] [Accepted: 09/19/2023] [Indexed: 10/05/2023]
Abstract
OBJECTIVE There is no study in the literature evaluating the dynamic thiol/disulfide homeostasis in patients with chronic venous insufficiency. Thus, we designed this study to evaluate the dynamic thiol/disulfide homeostasis as a novel indicator of oxidative stress in patients with chronic venous insufficiency. METHODS This was a prospective case-control study performed at the department of cardiovascular surgery of a tertiary referral hospital in Turkey. A total of 80 (CEAP C3-C6) patients with lower extremity chronic venous insufficiency (as the study group) and 80 healthy subjects (as the control group) were enrolled to the study. The participants' basic demographic and clinical characteristics as well as serum levels of some laboratory parameters including albumin, ferroxidase, myeloperoxidase, native thiol, total thiol, disulphide, native thiol/total thiol, disulphide/native thiol, and disulphide/total thiol were determined, and then compared between the groups. RESULTS In terms of basic demographic and clinical characteristics, both groups were statistically similar, and there were no significant differences between the groups. When the laboratory parameters were considered, serum ferroxidase and myeloperoxidase levels were detected to be significantly higher, whereas albumin, native thiol, total thiol, and disulphide levels were detected to be significantly lower in the study group than in the control group. CONCLUSIONS Dynamic thiol/disulphide homeostasis could be considered as an indicator reflecting the oxidative stress status in patients with chronic venous insufficiency.
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Affiliation(s)
- Ayhan Muduroglu
- Department of Cardiovascular Surgery, Bursa City Hospital, Bursa, Turkey.
| | - Yusuf Velioglu
- Department of Cardiovascular Surgery, Bursa City Hospital, Bursa, Turkey
| | - Humeyra Celik
- Department of Physiology, Bolu Abant Izzet Baysal University, Faculty of Medicine, Bolu, Turkey
| | - Ahmet Yuksel
- Department of Cardiovascular Surgery, Bursa City Hospital, Bursa, Turkey
| | - Mustafa Aldemir
- Department of Cardiovascular Surgery, Bursa Yuksek Ihtisas Training and Research Hospital, Bursa, Turkey
| | | | - Ali Onder Kilic
- Department of Cardiovascular Surgery, Bursa City Hospital, Bursa, Turkey
| | - Demir Cetintas
- Department of Cardiovascular Surgery, Bursa City Hospital, Bursa, Turkey
| | - Serdar Badem
- Department of Cardiovascular Surgery, Bursa City Hospital, Bursa, Turkey
| | - Mustafa Cagdas Cayir
- Department of Cardiovascular Surgery, Pamukkale University, Faculty of Medicine, Denizli, Turkey
| | - Gamze Gok
- Department of Biochemistry, Yildirim Beyazit University, Faculty of Medicine, Ankara, Turkey
| | - Ceylan Bal
- Department of Biochemistry, Yildirim Beyazit University, Faculty of Medicine, Ankara, Turkey
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İnce K, Güner S, Büyükbebeci A, İnce N. Are Thiol/Disulfide and 8-OHdG Levels in the Blood of Patients with Diabetic Foot a Risk Factor for Amputation? INT J LOW EXTR WOUND 2023:15347346231177858. [PMID: 37229661 DOI: 10.1177/15347346231177858] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 05/27/2023]
Abstract
OBJECTIVE As diabetic patients live longer and there are more effective treatments available, the incidence of diabetes and its complications is increasing. The effects of oxidative stress and antioxidant processes on diabetes behavior, especially diabetic foot, are really direct. The goal of this research is to investigate the effects of oxidative stress and antioxidant mechanism on the outcome of amputation by examining the levels of 8-hydroxy-2'-deoxyguanosine (8-OHdG) and thiol/disulfide in the blood of patients diagnosed with diabetic foot. METHODS The research consisted of 76 patients with type 2 diabetes with associated diabetic foot, ages 40-65 (51 men, 25 women). Patients with diabetic foot wounds and accompanying peripheral artery disease were not included in the study. After a mean follow-up of 9.6 months, 28 patients resulted in amputation. Between patients who required amputation and those who did not, the levels of 8-OHdG, native thiol, total thiol, disulfide, native thiol/total thiol ratio, disulfide/native thiol ratio, and disulfide/total thiol ratio were compared. Additionally, the age, sex, Wagner stage, and amputation outcome of these 2 patient groups were compared. RESULTS The outcomes of amputation in diabetic foot patients were not related to the levels of native thiol, total thiol, disulfide, native thiol/total thiol ratio, disulfide/native thiol ratio, total thiol/disulfide ratio, or 8-OHdG (P > .05). However, patients with diabetic foot who were male, older, and at a more advanced Wagner stage had a higher rate of amputations (P < .05). CONCLUSION Oxidative stress and antioxidant mechanism are effective on diabetes complications. However, since there are many factors affecting the outcome of amputation, they are not directly effective in terms of resulting amputation in patients with diabetic foot ulcer.
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Affiliation(s)
- Kamil İnce
- Department of Orthopaedics and Traumatology, T.C. Ministry of Health Islahiye State Hospital, Gaziantep, Turkey
| | - Savaş Güner
- Department of Orthopaedics and Traumatology, Gaziantep University, Faculty of Medicine, Gaziantep, Turkey
| | - Ayşegül Büyükbebeci
- Faculty of Medicine, Department of Biochemistry, Gaziantep University, Gaziantep, Turkey
| | - Nursima İnce
- Faculty of Physical Medicine and Rehabilitation, Hasan Kalyoncu Universty, Gaziantep, Turkey
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Cindoglu C, Uyanikoglu H, Esercan A, Eren MA, Koyuncu I, Sabuncu T. Evaluation of Nrg4 and thiol/disulfide homeostasis in patients with GDM. Eur J Obstet Gynecol Reprod Biol 2023; 285:105-109. [PMID: 37094427 DOI: 10.1016/j.ejogrb.2023.04.011] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 04/26/2023]
Abstract
BACKGROUND In this study, we aimed to evaluate the role of neuregulin4 (Nrg4) in the etiopathogenesis of gestational diabetes mellitus (GDM) and thiol/disulfide homeostasis as an indicator of oxidative stress. METHODS This prospective, case-control study included 34 women with diabetes and 34 healthy pregnant women who applied between January 2017 and January 2020. Levels of native and total thiol, disulfide and Nrg4 were measured in both diabetes mellitus and healthy pregnant groups. RESULTS When compared to the control group, the serum neuregulin4 levels in the diabetes group were considerably lower (3.22 ± 2.16 vs. 4.55 ± 0.96, p < 0.001). Native thiol (292.67 ± 43.65 vs. 366.40 ± 51.28; p < 0.001), total thiol (388.60 ± 46.60 vs. 414.52 ± 54.19; p < 0.001) levels and native thiol/total thiol ratio (75.51 ± 8.95 vs. 88.35 ± 3.54; p < 0.001) were lower in diabetes group compared to control group. Disulfide level (47.96 ± 19.52 vs. 24.06 ± 7.69) and disulfide/native thiol (17.13 ± 8.03, vs. 6.67 ± 2.30) and disulfide/total thiol (12.24 ± 4.47 vs. 5.82 ± 1.77) ratios were higher in diabetes group (all p < 0.001). CONCLUSIONS We suggested that decreased Nrg4 level and impaired oxidative stress parameters may be related with the increased risk of diabetes. However, we did not found a correlation between the Nrg4 and oxidative stress parameters.
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Affiliation(s)
- Cigdem Cindoglu
- Harran University Medical Faculty, Department of Internal Medicine, Sanliurfa, Turkey.
| | - Hacer Uyanikoglu
- Harran University Medical Faculty, Department of Obstetrics and Gynecology, Sanliurfa, Turkey
| | - Alev Esercan
- Sanliurfa Training and Research Hospital, Department of Obstetrics and Gynecology, Sanliurfa, Turkey
| | - Mehmet Ali Eren
- Harran University Medical Faculty, Department of Endocrinology, Şanlıurfa, Turkey
| | - Ismail Koyuncu
- Harran University Medical Faculty, Department of Biochemistry, Sanliurfa, Turkey
| | - Tevfik Sabuncu
- Harran University Medical Faculty, Department of Endocrinology, Şanlıurfa, Turkey
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Cindoglu C, Uyanikoglu H, Esercan A, Eren MA, Koyuncu I, Sabuncu T. Evaluation of Nrg4 and thiol/disulfide homeostasis in patients with GDM. Eur J Obstet Gynecol Reprod Biol X 2023. [DOI: 10.1016/j.eurox.2023.100193] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 04/03/2023] Open
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Hydrogen Sulfide Regulates Irisin and Glucose Metabolism in Myotubes and Muscle of HFD-Fed Diabetic Mice. Antioxidants (Basel) 2022; 11:antiox11071369. [PMID: 35883859 PMCID: PMC9311985 DOI: 10.3390/antiox11071369] [Citation(s) in RCA: 11] [Impact Index Per Article: 3.7] [Reference Citation Analysis] [Abstract] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 06/14/2022] [Revised: 07/04/2022] [Accepted: 07/13/2022] [Indexed: 12/26/2022] Open
Abstract
Irisin, a novel myokine, is secreted by the muscle following proteolytic cleavage of fibronectin type III domain containing 5 (FNDC5) and is considered a novel regulator of glucose homeostasis. Cystathionine γ-lyase (CSE) produces hydrogen sulfide (H2S) and is involved in glucose homeostasis. We examined the hypothesis that H2S deficiency leads to decreased FNDC5 and irisin secretion, and thereby alters glucose metabolism. High-fat diet-fed mice exhibited elevated blood glucose and significantly reduced levels of CSE, H2S, and PGC-1α, with decreased FNDC5/irisin levels and increased oxidative stress in the muscle compared with those of normal diet-fed mice (control). High glucose or palmitate decreases CSE/PGC-1α/FNDC5 levels and glucose uptake in myotubes. Inhibitors (propargylglycine and aminooxyacetate) of H2S producing enzymes or CSE siRNA significantly decreased levels of H2S and FNDC5 along with PGC-1α; similar H2S-deficient conditions also resulted in decreased GLUT4 and glucose uptake. The levels of H2S, PGC-1α, and FNDC5 and glucose uptake were significantly upregulated after treatment with l-cysteine or an H2S donor. Myoblast differentiation showed upregulation of PGC-1α and FNDC5, which was consistent with the increased expression of CSE/H2S. These findings suggest that the upregulation of H2S levels can have beneficial effects on glucose homeostasis via activation of the PGC-1α/FNDC5/irisin signaling pathway.
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Ozer Yaman S, Balaban Yucesan F, Orem A, Orem C, Vanizor Kural B, Yaman H. An increased disulfide/native thiol ratio and oxidative stress index in metabolic syndrome patients with postprandial lipemia. Int J Diabetes Dev Ctries 2022. [DOI: 10.1007/s13410-022-01095-y] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Submit a Manuscript] [Subscribe] [Scholar Register] [Indexed: 10/18/2022] Open
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Ischemia modified albumin and thiol/disulfide balance in patients with Hashimoto’s thyroiditis. UKRAINIAN BIOCHEMICAL JOURNAL 2022. [DOI: 10.15407/ubj94.01.114] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 11/17/2022] Open
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Leimkühler M, Bourgonje AR, van Goor H, Campmans-Kuijpers MJE, de Bock GH, van Leeuwen BL. Oxidative Stress Predicts Post-Surgery Complications in Gastrointestinal Cancer Patients. Ann Surg Oncol 2022; 29:10.1245/s10434-022-11412-8. [PMID: 35175456 PMCID: PMC9174134 DOI: 10.1245/s10434-022-11412-8] [Citation(s) in RCA: 1] [Impact Index Per Article: 0.3] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 09/01/2021] [Accepted: 01/18/2022] [Indexed: 01/10/2023]
Abstract
INTRODUCTION An excessive perioperative inflammatory reaction can lead to more postoperative complications in patients treated for gastrointestinal cancers. It has been suggested that this inflammatory reaction leads to oxidative stress. The most important nonenzymatic antioxidants are serum free thiols. The purpose of this study was to evaluate whether high preoperative serum free thiol levels are associated with short-term clinical outcomes. METHODS Blood samples were drawn before, at the end of, and 1 and 2 days after surgery of a consecutive series of patients with gastrointestinal cancer. Serum free thiols were detected using a colorimetric detection method using Ellman's reagent. Short-term clinical outcomes were defined as 30-day complications (Clavien-Dindo ≥2) and length of hospital stay. Logistic regression was applied to examine the association between serum free thiol levels and short-term patient outcomes. RESULTS Eighty-one patients surgically treated for gastrointestinal cancer were included in the study. Median age was 68 (range 26-87) years, and 28% were female. Patients in the lowest tertile of preoperative serum free thiols had a threefold higher risk to develop postoperative complications (odds ratio [OR]: 3.4; 95% confidence interval [CI]:1.1-10.7) and a fourfold higher risk to have an increased length of stay in the hospital (OR 4.0; 95% CI 1.3-12.9) compared with patients in the highest tertile. CONCLUSIONS Patients with lower preoperative serum free thiol levels, indicating a decrease in extracellular antioxidant capacity and therefore an increase in systemic oxidative stress, are more likely to develop postoperative complications and show a longer in hospital stay than patients with higher serum free thiol levels.
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Affiliation(s)
- M Leimkühler
- Department of Surgery, University of Groningen, University Medical Center Groningen, Groningen, The Netherlands
| | - A R Bourgonje
- Department of Gastroenterology and Hepatology, University of Groningen, University Medical Center Groningen, Groningen, The Netherlands
| | - H van Goor
- Department of Pathology and Medical Biology, University of Groningen, University Medical Center Groningen, Groningen, The Netherlands
| | - M J E Campmans-Kuijpers
- Department of Gastroenterology and Hepatology, University of Groningen, University Medical Center Groningen, Groningen, The Netherlands
| | - G H de Bock
- Department of Epidemiology, University of Groningen, University Medical Center Groningen, Groningen, The Netherlands
| | - B L van Leeuwen
- Department of Surgery, University of Groningen, University Medical Center Groningen, Groningen, The Netherlands.
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Ozben S, Kucuksayan E, Koseoglu M, Erel O, Neselioglu S, Ozben T. Plasma thiol/disulphide homeostasis changes in patients with relapsing-remitting multiple sclerosis. Int J Clin Pract 2021; 75:e14241. [PMID: 33891773 DOI: 10.1111/ijcp.14241] [Citation(s) in RCA: 2] [Impact Index Per Article: 0.5] [Reference Citation Analysis] [Abstract] [MESH Headings] [Track Full Text] [Journal Information] [Submit a Manuscript] [Subscribe] [Scholar Register] [Received: 03/14/2021] [Accepted: 04/16/2021] [Indexed: 01/01/2023] Open
Abstract
BACKGROUND Multiple sclerosis (MS) is a neuroinflammatory disease and inflammation and oxidative stress play important roles in its pathology. Thiol/disulphide homeostasis (TDH) is a special oxidative stress biomarker that has been found to be affected in several disorders including MS. There is no study demonstrating the effects of attack status of the relapsing-remitting multiple sclerosis (RRMS) patients on TDH levels. Our aim was to determine TDH levels in three different periods of RRMS patients and healthy individuals. METHODS The study was carried out in 29 patients with RRMS without a prior attack in the last twelve months (MS Control), 21 RRMS patients having a clinical acute attack within the last week (MS relapse), 12 of 21 MS relapse patients one month after the onset of attack and following 1000 mg methylprednisolone for 7 days (MS Remission) and 30 age- and sex-matched healthy individuals. TDH status was determined using an automated spectrophotometric analysis method. TDH levels in all patient groups and control subjects were compared with each other. RESULTS The lowest native thiol, total thiol levels and native thiol/total thiol ratio were found in the MS relapse patients in comparison to the MS control, MS remission groups and healthy controls. In contrast, disulphide levels, disulphide/native thiol and disulphide/total thiol ratios were highest in the MS relapse group compared to the other patient groups and healthy subjects. CONCLUSION Our findings indicate that increased oxidative stress in RRMS patients is reflected with decreased native and total thiol and increased disulphide levels. Since the formation of disulphide bonds is reversible, the progression of RRMS involving abnormal TDH may be controlled, converting disulphides to thiols. So, we suggest determining the dynamic TDH status as a novel and special biomarker in the diagnosis and prognosis of the RRMS patients.
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Affiliation(s)
- Serkan Ozben
- Department of Neurology, Antalya Training and Research Hospital, Antalya, Turkey
| | - Ertan Kucuksayan
- Department of Medical Biochemistry, Medical Faculty, Alanya Alaaddin Keykubat University (ALKU), Alanya, Antalya, Turkey
| | - Mesrure Koseoglu
- Department of Neurology, Bakirkoy Psychiatry and Neurology Research and Training Hospital, Istanbul, Turkey
| | - Ozcan Erel
- Department of Biochemistry, Medical Faculty, Yildirim Beyazit University, Ankara, Turkey
| | - Salim Neselioglu
- Department of Biochemistry, Medical Faculty, Yildirim Beyazit University, Ankara, Turkey
| | - Tomris Ozben
- Department of Biochemistry, Medical Faculty, Akdeniz University, Antalya, Turkey
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Özsürekci C, Şengül Ayçiçek G, Çalışkan H, Tuna Doğrul R, Neşelioğlu S, Özcan M, Doğu BB, Cankurtaran M, Erel Ö, Halil MG. Thiol-disulfide homeostasis and ischemia-modified albumin as a marker of oxidative stress in patients with sarcopenia. Geriatr Gerontol Int 2021; 21:584-589. [PMID: 34080286 DOI: 10.1111/ggi.14199] [Citation(s) in RCA: 5] [Impact Index Per Article: 1.3] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 03/04/2021] [Revised: 05/02/2021] [Accepted: 05/08/2021] [Indexed: 11/26/2022]
Abstract
AIM Sarcopenia is characterized by progressive and generalized loss of skeletal muscle mass and strength. Chronic inflammatory conditions and increased oxidative stress are in the pathogenesis of sarcopenia. Our aim was to evaluate the relationship between sarcopenia and thiol-disulfide homeostasis and ischemia-modified albumin levels as an oxidative stress marker. METHODS Patients aged ≥65 years were recruited in this study. Sarcopenia was diagnosed according to the European Working Group on Sarcopenia in Older People criterion. Total thiol, native thiol, disulfide and ischemia-modified albumin levels were measures according to clinical and laboratory features. Patients were divided into two groups according to their sarcopenia presence; thiol-disulfide homeostasis and ischemia-modified albumin levels were evaluated between these groups. RESULTS Overall, 94 patients were analyzed. The mean age was 75.0 ± 6.71 years. A total of 39% of the patients were diagnosed as probable sarcopenia, 3.2% had sarcopenia, 6.4% had severe sarcopenia and 51.1% were diagnosed as normal. The levels of native thiol, total thiol, disulfide level and disulfide-native thiol, native thiol-total thiol and disulfide-total thiol ratios were similar in patients with sarcopenia when compared with the control group. In addition, there were no differences between albumin and ischemia-modified albumin levels. In univariate regression analysis, handgrip strength was found to be an independent predictor of native thiol and total thiol, and disulfide levels. CONCLUSION This is the first study in the literature that evaluates the thiol-disulfide homeostasis and ischemia-modified albumin levels in sarcopenic older patients. Long-term studies are warranted to confirm the relationship between oxidative stress markers and sarcopenia. Geriatr Gerontol Int 2021; 21: 584-589.
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Affiliation(s)
- Cemile Özsürekci
- Division of Geriatric Medicine, Department of Internal Medicine, Hacettepe University Faculty of Medicine, Ankara, Turkey
| | - Gözde Şengül Ayçiçek
- Division of Geriatric Medicine, Department of Internal Medicine, Hacettepe University Faculty of Medicine, Ankara, Turkey
| | - Hatice Çalışkan
- Division of Geriatric Medicine, Department of Internal Medicine, Hacettepe University Faculty of Medicine, Ankara, Turkey
| | - Rana Tuna Doğrul
- Division of Geriatric Medicine, Department of Internal Medicine, Hacettepe University Faculty of Medicine, Ankara, Turkey
| | - Salim Neşelioğlu
- Department of Clinical Biochemistry, Yıldırım Beyazit University Faculty of Medicine, Ankara, Turkey
| | - Münevver Özcan
- Division of Geriatric Medicine, Department of Internal Medicine, Hacettepe University Faculty of Medicine, Ankara, Turkey
| | - Burcu B Doğu
- Division of Geriatric Medicine, Department of Internal Medicine, Hacettepe University Faculty of Medicine, Ankara, Turkey
| | - Mustafa Cankurtaran
- Division of Geriatric Medicine, Department of Internal Medicine, Hacettepe University Faculty of Medicine, Ankara, Turkey
| | - Özcan Erel
- Department of Clinical Biochemistry, Yıldırım Beyazit University Faculty of Medicine, Ankara, Turkey
| | - Meltem G Halil
- Division of Geriatric Medicine, Department of Internal Medicine, Hacettepe University Faculty of Medicine, Ankara, Turkey
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Ma Q, Li XL, Qi C, Zhang M, Zheng Y, Shi Q, Jin Y, Di L, Nan J, Min JZ. Simultaneous Determination of Chiral Thiol Compounds and Monitoring of Dynamic Changes in Human Urine after Drinking Chinese Korean Ethnic Rice Wine. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 2021; 69:5416-5427. [PMID: 33908777 DOI: 10.1021/acs.jafc.0c07870] [Citation(s) in RCA: 6] [Impact Index Per Article: 1.5] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Subscribe] [Scholar Register] [Indexed: 06/12/2023]
Abstract
Chinese Korean ethnic rice wine, a traditional fermented wine made from rice or corn, has antioxidant and antihypertensive activities. Although the determination of amino acids and other nutrients in rice wine has been reported, the existence of chiral thiol compounds has not been published in the literature. Therefore, we established a highly sensitive and selective ultrahigh-performance liquid chromatography-high-resolution mass spectrometry method for simultaneous determination and chiral separation of dl-Cys-GSH, dl-Cys-Cys, and dl-Cys-Hcy based on (R)-(5-(3-isothiocyanatopyrrolidin-1-yl)-5-oxopentyl) triphenylphosphonium derivatization. Three thiol diastereomers were completely separated on a YMC Triart C18 (2.0 × 150 mm, 1.9 μm) column with a resolution value (Rs) ≥ 1.52. The correlation coefficients were ≥0.9996, limit of detection was 2.40-7.20 fmol, and mean recoveries were 83.33-98.59%. Furthermore, fitted curves for dynamic changes in three kinds of chiral thiols in 10 human urine samples after drinking rice wine were drawn. Meanwhile, the metabolic changes in d/l-thiol compounds in human urine were investigated.
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Affiliation(s)
- Qingkun Ma
- Key Laboratory of Natural Medicines of the Changbai Mountain, Ministry of Education, Pharmaceutical Analysis, College of Pharmacy Yanbian University, and Department of Pharmacy, Department of Orthopaedics, Yanbian University Hospital, Yanji 133002, Jilin Province, China
| | - Xi-Ling Li
- Key Laboratory of Natural Medicines of the Changbai Mountain, Ministry of Education, Pharmaceutical Analysis, College of Pharmacy Yanbian University, and Department of Pharmacy, Department of Orthopaedics, Yanbian University Hospital, Yanji 133002, Jilin Province, China
| | - Chao Qi
- Key Laboratory of Natural Medicines of the Changbai Mountain, Ministry of Education, Pharmaceutical Analysis, College of Pharmacy Yanbian University, and Department of Pharmacy, Department of Orthopaedics, Yanbian University Hospital, Yanji 133002, Jilin Province, China
| | - Minghui Zhang
- Key Laboratory of Natural Medicines of the Changbai Mountain, Ministry of Education, Pharmaceutical Analysis, College of Pharmacy Yanbian University, and Department of Pharmacy, Department of Orthopaedics, Yanbian University Hospital, Yanji 133002, Jilin Province, China
| | - Yan Zheng
- Key Laboratory of Natural Medicines of the Changbai Mountain, Ministry of Education, Pharmaceutical Analysis, College of Pharmacy Yanbian University, and Department of Pharmacy, Department of Orthopaedics, Yanbian University Hospital, Yanji 133002, Jilin Province, China
| | - Qing Shi
- Key Laboratory of Natural Medicines of the Changbai Mountain, Ministry of Education, Pharmaceutical Analysis, College of Pharmacy Yanbian University, and Department of Pharmacy, Department of Orthopaedics, Yanbian University Hospital, Yanji 133002, Jilin Province, China
| | - Yueying Jin
- Key Laboratory of Natural Medicines of the Changbai Mountain, Ministry of Education, Pharmaceutical Analysis, College of Pharmacy Yanbian University, and Department of Pharmacy, Department of Orthopaedics, Yanbian University Hospital, Yanji 133002, Jilin Province, China
| | - Lei Di
- Key Laboratory of Natural Medicines of the Changbai Mountain, Ministry of Education, Pharmaceutical Analysis, College of Pharmacy Yanbian University, and Department of Pharmacy, Department of Orthopaedics, Yanbian University Hospital, Yanji 133002, Jilin Province, China
| | - Jun Nan
- Key Laboratory of Natural Medicines of the Changbai Mountain, Ministry of Education, Pharmaceutical Analysis, College of Pharmacy Yanbian University, and Department of Pharmacy, Department of Orthopaedics, Yanbian University Hospital, Yanji 133002, Jilin Province, China
| | - Jun Zhe Min
- Key Laboratory of Natural Medicines of the Changbai Mountain, Ministry of Education, Pharmaceutical Analysis, College of Pharmacy Yanbian University, and Department of Pharmacy, Department of Orthopaedics, Yanbian University Hospital, Yanji 133002, Jilin Province, China
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Otal Y, Kahraman FA, Haydar FG, Erel Ö. Dynamic thiol/disulfide homeostasis as oxidative stress marker in diabetic ketoacidosis. Turk J Med Sci 2021; 51:743-748. [PMID: 33356023 PMCID: PMC8203137 DOI: 10.3906/sag-1904-55] [Citation(s) in RCA: 2] [Impact Index Per Article: 0.5] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 04/08/2019] [Accepted: 12/26/2020] [Indexed: 12/28/2022] Open
Abstract
Background/aim The aim of present study was to investigate the dynamic thiol/disulfide homeostasis as oxidative stress marker in diabetic ketoacidosis (DKA). Materials and methods A total of 77 participants consisting of 32 patients with DKA and 45 healthy volunteers were included in the study. Thiol/disulfide homeostasis (TDH) [total thiol-native thiol/disulfide changes] were measured in both groups (patient group and control group) using a brand new method developed by Erel and Neselioglu. Half of the difference between total thiol and native thiol concentrations gives the amount of disulfide bond.
Results Total thiol, native thiol, and disulfide levels in blood were found to be low. The levels of total thiol (P < 0.001) and native thiol (P < 0.001 ) were significantly lower in patients with DKA than in the control group. At the same time, the level of disulfide was nonsignificantly lower in the patient group than the control group (P = 0.388). The level of IMA was higher in the patient group than in the control group (P < 0.001). Conclusion The total thiol, native thiol, and disulfide levels in DKA decrease in favor of oxidative stress.
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Affiliation(s)
- Yavuz Otal
- Department of Emergency Medicine, Ankara City Hospital, Ankara, Turkey
| | - Fatih Ahmet Kahraman
- Department of Emergency Medicine, Faculty of Medicine, Yıldırım Beyazıt University, Ankara, Turkey
| | | | - Özcan Erel
- Department of Medical Biochemistry, Faculty of Medicine, Yıldırım Beyazıt University, Ankara, Turkey
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Yavaş MC, Yegin K, Oruç S, Delen K, Sirav B. Analysis of thiol/disulphide homeostasis and oxidant-antioxidant status as a result of exposure to radio-frequency electromagnetic fields. Electromagn Biol Med 2021; 40:84-91. [PMID: 33459076 DOI: 10.1080/15368378.2021.1874970] [Citation(s) in RCA: 2] [Impact Index Per Article: 0.5] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 08/24/2020] [Accepted: 11/25/2020] [Indexed: 10/22/2022]
Abstract
The aim of the current study is to investigate the effect of daily long-term radio-frequency electromagnetic field (RF-EMFs) exposure on thiol/disulphide homeostasis and oxidant-antioxidant status in the serum of rats. Fourteen male Sprague Dawley rats were divided into two equal groups (n = 7). They were grouped as group 1 (sham-control) and group 2 (2100 MHz exposed). Group 2 rats were exposed to GSM-like signals for 5 h/day, 7 days per week for 14 days. In serum of blood samples, the native, total and native/total thiol levels and antioxidant-oxidant parameters were analyzed. The study revealed that the mean serum total thiol levels of GSM exposure group was rather higher than sham-control group (p = 0,219); the mean serum native thiol levels of GSM exposure group was rather higher than sham-control group (p = 0,416), and the mean serum disulphide levels of GSM exposure group was rather higher than sham-control group (p = 0,566). On the other hand, the mean serum total antioxidant status (TAS), total oxidant status (TOS) and oxidative stress index (OSI) of GSM exposure group were higher than sham-control group. There are no data in the literature, that examine the effects of RF-EMFs on thiol/disulphide homeostasis. The results of this study, indicate that RF-EMFs did not cause oxidative stress and statistically changes in thiol/disulphide homeostasis. More sample sizes and longer-term exposures are needed to confirm the results of this study.
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Affiliation(s)
- Mehmet Cihan Yavaş
- Department of Biophysics, Faculty of Medicine, Kırşehir Ahi Evran University , Kırşehir, Turkey
| | - Korkut Yegin
- Department of Electrical and Electronics Engineering, Ege University , Izmir, Turkey
| | - Sinem Oruç
- Department of Biophysics, Faculty of Medicine, Gazi University , Ankara, Turkey
| | - Kevser Delen
- Department of Biophysics, Faculty of Medicine, Gazi University , Ankara, Turkey
| | - Bahriye Sirav
- Department of Biophysics, Faculty of Medicine, Gazi University , Ankara, Turkey
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Alışık M, Işik MU. The Relationship between Choroidal Thickness and Intracellular Oxidised-reduced Glutathione and Extracellular Thiol-disulfide Homeostasis at Different Stages of Diabetic Retinopathy. Curr Eye Res 2020; 46:367-372. [PMID: 33103488 DOI: 10.1080/02713683.2020.1842463] [Citation(s) in RCA: 6] [Impact Index Per Article: 1.2] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 10/23/2022]
Abstract
Purpose: To evaluate the relationship between diabetic retinopathy and oxidative damage by measuring intracellular and extracellular thiol levels, and to compare intracellular and extracellular thiol levels. Method: In this prospective, cross-sectional, and comparative study, 25 healthy control participants (group 1), a total of 25 diabetic macular edema (DME) patients with non-proliferative diabetic retinopathy (DRP) and without DME (group 2), and 25 DME patients with non-proliferative DRP and with DME (group 3) were included. Choroidal thickness (ChT) and central macular thickness (CMT) were measured by spectral domain optic coherence tomography. For the evaluation of antioxidant/oxidant balance, intracellular GSH (reduced glutathione) and GSSG (oxidized glutathione), extracellular SH (thiol) and SS (disulfide) levels were measured and recorded. Results: Comparing intracellular and extracellular thiol levels between groups, intracellular GSSG level and GSSG/GSH percent ratio, and extracellular disulfide and SS/SH percent ratio values were higher in diabetic patients than healthy participants. Choroidal thicknesses were significantly thinner in DRP groups compared to the healthy population. When the relationship between choroidal thicknesses and thiol levels was investigated, there were significant relationships between choroidal thicknesses and thiol levels in group 3. Conclusion: Oxidative stress and impaired intracellular GSH/GSSG and serum SH/SS balances were observed to have an effect on DRP and DME pathogenesis. In addition, in groups with and without DME, thinning in choroidal thicknesses and the relationship between these thicknesses and intra/extracellular oxidative stress indicators can also be explained.
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Affiliation(s)
- Murat Alışık
- Department of Biochemistry, Bolu Abant İzzet Baysal University , Bolu, Turkey
| | - Mehmed Uğur Işik
- Department of Ophthalmology, Kastamonu University Faculty of Medicine , Kastamonu, Turkey
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Erel Ö, Erdoğan S. Thiol-disulfide homeostasis: an integrated approach with biochemical and clinical aspects. Turk J Med Sci 2020; 50:1728-1738. [PMID: 32233181 PMCID: PMC7672356 DOI: 10.3906/sag-2003-64] [Citation(s) in RCA: 44] [Impact Index Per Article: 8.8] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Journal Information] [Subscribe] [Scholar Register] [Received: 03/08/2020] [Accepted: 03/30/2020] [Indexed: 12/11/2022] Open
Abstract
Dynamic thiol-disulfide homeostasis (TDH) is a new area has begun to attract more scrutiny. Dynamic TDH is reversal of thiol oxidation in proteins and represents the status of thiols (-SH) and disulfides (-S-S-). Organic compounds containing the sulfhydryl group is called thiol, composed of sulfur and hydrogen atoms. Disulfides are the most important class of dynamic, redox responsive covalent bonds build in between two thiol groups. For many years, thiol levels were analyzed by several methods. During last years, measurements of disulfide levels have been analyzed by a novel automated method, developed by Erel and Neselioglu. In this method, addition to thiol (termed as native thiol) levels, disulfide levels were also measured and sum of native thiol and disulfide levels were termed as total thiol. Therefore, TDH was begun to be understood in organism. In healthy humans, TDH is maintained within a certain range. Dysregulated dynamic TDH has been implicated several disorders with unknown etiology. A growing body of evidence has demonstrated that the thiol-disulfide homeostasis is involved in variety diseases, such as diabetes mellitus, hypertension, nonsmall cell lung cancer, familial Mediterranean fever (FMF), inflammatory bowel diseases, occupational diseases, gestational diabetes mellitus and preeclampsia. These results may elucidate some pathogenic mechanism or may be a predictor indicating diagnostic clue, prognostic marker or therapeutic sign. In conclusion, protection of the thiol-disulfide homeostasis is of great importance for the human being. Evidence achieved so far has proposed that thiol-disulfide homeostasis is an important issue needs to elucidate wholly.
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Affiliation(s)
- Özcan Erel
- Department of Medical Biochemistry, Faculty of Medicine, Ankara Yıldırım Beyazıt Universty, Ankara , Turkey,Department of Medical Biochemistry, Ankara City Hospital , Ankara, Turkey
| | - Serpil Erdoğan
- Department of Medical Biochemistry, Faculty of Medicine, Ankara Yıldırım Beyazıt Universty, Ankara , Turkey
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Kalayci M, Cetinkaya E, Yigit K, Sabaner MC, Duman R, Balik AR, Erel Ö. Ischemia-Modified Albumin Levels and Thiol-Disulphide Homeostasis in Diabetic Macular Edema in Patients with Diabetes Mellitus Type 2. Curr Eye Res 2020; 46:683-688. [PMID: 32878498 DOI: 10.1080/02713683.2020.1818261] [Citation(s) in RCA: 3] [Impact Index Per Article: 0.6] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 10/23/2022]
Abstract
PURPOSE It was aimed to assess the role of thiol-disulphide homeostasis and ischemia-modified albumin (IMA) level in the development of diabetic macular edema (DME) in patients with diabetes mellitus type 2 (T2DM). MATERIALS AND METHODS Sixty-six study patients were divided into two groups. Group I included 43 patients with T2DM and DME, and Group 2 included 23 patients with T2DM without eye involvement. A novel colorimetric method was used to assess thiol-disulphide homeostasis. Between the two groups IMA, total anti-oxidant, and total oxidant levels were measured and compared. RESULTS In Group 1, total and native thiol levels and disulphide levels were lower compared to Group 2 (p = .025, p < .001 and p = .013, respectively). Disulphide/native thiol, disulphide/total thiol ratios and native thiol/total thiol were similar between the groups. Total anti-oxidant level (TAL) reduced whereas total oxidant level (TOL) increased in Group 1 compared to Group 2 (p = .001, p = .002, respectively). Albumin level decreased, whereas IMA level increased in Group 1 compared to Group 2 (p < .001 for both). CONCLUSIONS The disruption in thiol/disulphide homeostasis, increased IMA and oxidative stress have an impact on the development of diabetic macular edema.
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Affiliation(s)
- Mustafa Kalayci
- Department of Ophthalmology, Antalya Training and Research Hospital, Antalya, Turkey
| | - Ersan Cetinkaya
- Department of Ophthalmology, Antalya Training and Research Hospital, Antalya, Turkey
| | - Kenan Yigit
- Department of Ophthalmology, Antalya Training and Research Hospital, Antalya, Turkey
| | | | - Reşat Duman
- Department of Opthalmology, Bursa City Hospital, Bursa, Turkey
| | - Ahmet Rifat Balik
- Department of Biochemistry, Yıldırım Beyazıt University, Ankara, Turkey
| | - Özcan Erel
- Department of Biochemistry, Yıldırım Beyazıt University, Ankara, Turkey
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Buyukaydin B, Guler EM, Karaaslan T, Olgac A, Zorlu M, Kiskac M, Kocyigit A. Relationship between diabetic polyneuropathy, serum visfatin, and oxidative stress biomarkers. World J Diabetes 2020; 11:309-321. [PMID: 32843933 PMCID: PMC7415233 DOI: 10.4239/wjd.v11.i7.309] [Citation(s) in RCA: 5] [Impact Index Per Article: 1.0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Journal Information] [Submit a Manuscript] [Subscribe] [Scholar Register] [Received: 12/30/2019] [Revised: 05/08/2020] [Accepted: 05/13/2020] [Indexed: 02/06/2023] Open
Abstract
BACKGROUND Diabetic polyneuropathy is a very common complication of diabetes. Numerous studies are available in terms of pathogenesis. But examination methods with low reliability are still not standardized and generally time consuming. High-sensitive, easy-to-access methods are expected. Biochemical markers are one of the subjects of research. We aimed to discover a potential biomarker that can be used for this purpose in patients with diabetes who have not yet developed symptoms of neuropathy.
AIM To determine the place and availability of visfatin and thiol-disulfide homeostasis in this disorder.
METHODS A total of 392 patients with type 2 diabetes mellitus were included in the study. The polyneuropathy clinical signs were evaluated with the Subjective Peripheral Neuropathy Screen Questionnaire and Michigan Neuropathy Screening Instrument questionnaire and examination. The biochemical parameters, oxidative stress markers, visfatin, and thiol-disulfide homeostasis were analyzed and correlated with each other and clinical signs.
RESULTS Subjective Peripheral Neuropathy Screen Questionnaire and Michigan Neuropathy Screening Instrument questionnaire with examination scores were correlated with each other and diabetes duration (P < 0.005). Neuropathy related symptoms were present in 20.7% of the patients, but neuropathy related findings were observed in 43.9% of the patients. Serum glucose, glycated hemoglobin, and visfatin were positively correlated with each other. Also, these parameters were positively correlated with the total oxidative stress index. Total and native thiol was positively correlated with total antioxidant status and negatively with oxidant status. Inversely thiol-disulfide positively correlated with higher glucose and oxidant status and negatively with total antioxidant status (P < 0.005). There was no correlation between visfatin and thiol-disulphide (P = 0.092, r = 0.086). However, a significant negative correlation was observed between visfatin and total with native thiol (P < 0.005, r = -0.338), (P < 0.005, r = -0.448).
CONCLUSION Diagnosis of neuropathy is one of the issues studied in patients with diabetes. Visfatin and thiol-disulfide balance were analyzed for the first time in this study with inspiring results.
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Affiliation(s)
- Banu Buyukaydin
- Department of Internal Medicine, Bezmialem Vakif University Medical Faculty, İstanbul 34093, Turkey
| | - Eray Metin Guler
- Department of Medical Biochemistry, Bezmialem Vakif University Medical Faculty, İstanbul 34093, Turkey
| | - Tahsin Karaaslan
- Department of Nephrology, Istanbul Medeniyet University Medical Faculty, İstanbul 34093, Turkey
| | - Atilla Olgac
- Department of Internal Medicine, Bezmialem Vakif University Medical Faculty, İstanbul 34093, Turkey
| | - Mehmet Zorlu
- Department of Internal Medicine, Bezmialem Vakif University Medical Faculty, İstanbul 34093, Turkey
| | - Muharrem Kiskac
- Department of Internal Medicine, Bezmialem Vakif University Medical Faculty, İstanbul 34093, Turkey
| | - Abdurrahim Kocyigit
- Department of Medical Biochemistry, Bezmialem Vakif University Medical Faculty, İstanbul 34093, Turkey
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Mengen E, Uçaktürk SA, Kocaay P, Kaymaz Ö, Neşelioğlu S, Erel Ö. The Significance of Thiol/Disulfide Homeostasis and Ischemia-modified Albumin Levels in Assessing Oxidative Stress in Obese Children and Adolescents. J Clin Res Pediatr Endocrinol 2020; 12:45-54. [PMID: 31414586 PMCID: PMC7127896 DOI: 10.4274/jcrpe.galenos.2019.2019.0039] [Citation(s) in RCA: 7] [Impact Index Per Article: 1.4] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Submit a Manuscript] [Subscribe] [Scholar Register] [Received: 03/07/2019] [Accepted: 08/07/2019] [Indexed: 12/17/2022] Open
Abstract
Objective There is an association between obesity and several inflammatory and oxidative markers in children. In this study, we analyzed thiol/disulfide homeostasis and serum ischemia-modified albumin (IMA) levels for the first time in order to clarify and determine the oxidant/antioxidant balance in metabolically healthy and unhealthy children. Methods This study included obese children and healthy volunteers between 4-18 years of age. The obese patients were divided into two groups: metabolically healthy obese (MHO) and metabolically unhealthy obese (MUO). Biochemical parameters including thiol/disulfide homeostasis, and IMA concentrations were analyzed. Results There were 301 recruits of whom 168 (55.8%) were females. The obese children numbered 196 (MHO n=58 and MUO n=138) and healthy controls numbered 105. No statistically significant difference could be found in ages and genders of the patients among all groups (p>0.05, for all). Native thiol (SH), total thiol (SH+SS), and native thiol/total thiol (SH/SH+SS) ratio were statistically significantly lower in the MUO group than the control group (p<0.001, p=0.005, and p=0.005; respectively). Disulfide (SS), disulfide/native thiol (SS/SH), disulfide/total thiol (SS/SH+SS) and IMA levels were statistically significantly higher in the MUO group than the control group (p=0.002, p<0.001, p<0.001, and p=0.001, respectively). Conclusion Chronic inflammation due to oxidative stress induced by impaired metabolic parameters in MUO children caused impairment in thiol redox homeostasis. Our data suggested that the degree of oxidant imbalance in obese children worsened as obesity and metabolic abnormalities increased. It is hypothesized that thiol/disulfide homeostasis and high serum IMA levels may be reliable indicators of oxidant-antioxidant status in MUO children.
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Affiliation(s)
- Eda Mengen
- Ankara City Hospital, Children’s Hospital, Clinic of Pediatric Endocrinology, Ankara, Turkey
| | - Seyit Ahmet Uçaktürk
- Ankara City Hospital, Children’s Hospital, Clinic of Pediatric Endocrinology, Ankara, Turkey
| | - Pınar Kocaay
- Ankara City Hospital, Children’s Hospital, Clinic of Pediatric Endocrinology, Ankara, Turkey
| | - Özlem Kaymaz
- Ankara University Faculty of Medicine, Depatment of Statistics, Ankara, Turkey
| | - Salim Neşelioğlu
- Yıldırım Beyazıt University Faculty of Medicine, Department of Biochemistry, Ankara, Turkey
| | - Özcan Erel
- Yıldırım Beyazıt University Faculty of Medicine, Department of Biochemistry, Ankara, Turkey
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Sandikci SC, Colak S, Omma A, Enecik ME, Ozbalkan Z, Neselioglu S, Erel O. An investigation of thiol/disulfide homeostasis in patients with Behçet's disease. Arch Med Sci 2020; 16:1353-1359. [PMID: 33224334 PMCID: PMC7667443 DOI: 10.5114/aoms.2019.86703] [Citation(s) in RCA: 1] [Impact Index Per Article: 0.2] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Journal Information] [Submit a Manuscript] [Subscribe] [Scholar Register] [Received: 07/19/2018] [Accepted: 09/28/2018] [Indexed: 11/17/2022] Open
Abstract
INTRODUCTION Behçet's disease (BD) is a relapsing systemic inflammatory disorder. The diagnosis of BD is primarily based on clinical findings. Current biomarkers are not yet sufficient to diagnose and cannot anticipate the course of the disease and response to treatment. The aim of this study was to evaluate the relationship between the thiol-disulfide balance and disease activity and organ involvement in BD. MATERIAL AND METHODS A hundred fifty patients with BD and 100 age- and gender-matched healthy controls were included in the study. Disease activity was assessed with the BD Current Activity form score. Serum levels of native thiol (NT), total thiol (TT), and disulfide were measured and the disulfide/native thiol, disulfide/total thiol and native thiol/total thiol levels were calculated for the patient and control groups. RESULTS Native thiol, total thiol, native thiol/total thiol values of the BD patients were significantly lower than those of the control group. The disulfide/native thiol, disulfide/total thiol values of BD patients were higher compared to the control group and the disulfide value of the BD group was slightly higher compared to the control group. No correlation was determined between thiol levels and disease activity and organ involvement in BD. CONCLUSIONS In patients with Behcet's disease, the thiol-disulfide homeostasis balance shifted towards disulfide formation due to thiol oxidation. It may be used as a novel marker in BD because it is easy, practical, fully automated and relatively inexpensive.
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Affiliation(s)
- Sevinc Can Sandikci
- Ankara Dışkapı Yıldırım Beyazıt Training and Research Hospital, Ankara, Turkey
| | - Seda Colak
- Ankara Numune Training and Research Hospital, Ankara, Turkey
| | - Ahmet Omma
- Ankara Numune Training and Research Hospital, Ankara, Turkey
| | | | - Zeynep Ozbalkan
- Ankara Numune Training and Research Hospital, Ankara, Turkey
| | - Salim Neselioglu
- Department of Clinical Biochemistry, Faculty of Medicine, Ankara Yildirim Beyazit University, Ankara, Turkey
| | - Ozcan Erel
- Department of Clinical Biochemistry, Faculty of Medicine, Ankara Yildirim Beyazit University, Ankara, Turkey
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The effect of thiol-disulfide homeostasis in patients undergoing on-pump coronary artery bypass grafting. TURK GOGUS KALP DAMAR CERRAHISI DERGISI-TURKISH JOURNAL OF THORACIC AND CARDIOVASCULAR SURGERY 2019; 27:484-492. [PMID: 32082914 DOI: 10.5606/tgkdc.dergisi.2019.18033] [Citation(s) in RCA: 6] [Impact Index Per Article: 1.0] [Reference Citation Analysis] [Abstract] [Track Full Text] [Subscribe] [Scholar Register] [Received: 03/12/2019] [Accepted: 07/15/2019] [Indexed: 12/27/2022]
Abstract
Background In this study, we aimed to investigate the effects of thiol-disulfide homeostasis in patients undergoing on-pump coronary artery bypass grafting. Methods Between January 2018 and October 2018, a total of 51 patients (43 males, 8 females; mean age 61.8±8.7 years; range, 38 to 78 years) who underwent isolated on-pump coronary artery bypass grafting were included. Thiol-disulfide homeostasis parameters were studied in the preoperative period (T1), 30 min after the removal of cross-clamp (T2), and postoperative sixth h (T3). Hemodynamic parameters such as atrial fibrillation and inotropic support requirement of the patients were evaluated in the postoperative period. Results There were significant differences in the measured thiol-disulfide homeostasis parameters at different time points of surgery (p<0.001). Binary logistic regression analysis showed that T2-disulfide/native thiol ratio was an independent predictor of the development of postoperative atrial fibrillation (p=0.042). There were positive and significant correlations between the T2-disulfide levels and cross-clamp time (r:0.307, p=0.029). Conclusion Thiol-disulfide homeostasis in patients undergoing on-pump coronary artery bypass grafting changes toward to disulfide. Disulfide levels increase in parallel with prolonged ischemia time. Decreased native thiol and increased disulfide levels during ischemic period may be predictive of postoperative atrial fibrillation.
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Eroglu H, Turgal M, Senat A, Karakoc G, Neselioglu S, Yucel A. Maternal and fetal thiol/disulfide homeostasis in fetal growth restriction. J Matern Fetal Neonatal Med 2019; 34:1658-1665. [PMID: 31327276 DOI: 10.1080/14767058.2019.1646239] [Citation(s) in RCA: 13] [Impact Index Per Article: 2.2] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 12/21/2022]
Abstract
PURPOSE To evaluate thiol/disulfide homeostasis in both maternal and fetal compartment in the presence of fetal growth restriction (FGR). MATERIALS AND METHODS A prospective case-control study was carried out in women with FGR (n: 40) or normally growing fetus (n: 40). FGR was defined as estimated fetal weight below the 10th percentile for the gestational age. Maternal serum and fetal cord blood samples were collected from all participants and native thiol-disulfide exchanges were examined with automated method enabling the measurement of both sides of thiol-disulfide balance. RESULTS Native thiol, total thiol and disulfide amounts were decreased in the maternal serum and fetal cord blood of babies born to women with FGR (p < .05). The most sensitive marker was maternal native thiol (82.5, 95% confidence interval, 67.22-92.66%), while the maternal total thiol had highest specificity value (77.5, 95% confidence interval, 61.55-89.16%). CONCLUSIONS Maternal and fetal serum thiol/disulfide profiles may use prediction of FGR severity and its neonatal outcome.
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Affiliation(s)
- Hasan Eroglu
- Maternal Fetal Medicine, Etlik Zubeyde Hanim Kadin Hastaliklari Egitim ve Arastirma Hastanesi, Ankara, Turkey
| | - Mert Turgal
- Obstetrics and Gynecology, Koc University Hospital, Topkapi, Turkey
| | - Almila Senat
- Biochemistry, Faculty of Medicine, Yildirim Beyazit University, Ankara, Turkey
| | - Gokhan Karakoc
- Maternal Fetal Medicine, Etlik Zubeyde Hanim Kadin Hastaliklari Egitim ve Arastirma Hastanesi, Ankara, Turkey
| | - Salim Neselioglu
- Biochemistry, Yildirim Beyazit Universitesi Tip Fakultesi, Ankara, Turkey
| | - Aykan Yucel
- Maternal Fetal Medicine, Etlik Zubeyde Hanim Kadin Hastaliklari Egitim ve Arastirma Hastanesi, Ankara, Turkey
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Yaşar Durmuş S, Şahin NM, Ergin M, Neşelioğlu S, Aycan Z, Erel Ö. How does thiol/disulfide homeostasis change in children with type 1 diabetes mellitus? Diabetes Res Clin Pract 2019; 149:64-68. [PMID: 30710657 DOI: 10.1016/j.diabres.2019.01.027] [Citation(s) in RCA: 4] [Impact Index Per Article: 0.7] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Journal Information] [Submit a Manuscript] [Subscribe] [Scholar Register] [Received: 08/04/2018] [Revised: 12/24/2018] [Accepted: 01/21/2019] [Indexed: 12/23/2022]
Abstract
AIMS An increase in reactive oxygen species leads to formation of covalent bonds between sulfur atoms, thus thiol/disulfide homeostasis shifts towards the disulfide direction and oxidative damage occurs. We aimed to determine thiol/disulfide homeostasis in children with T1DM. METHODS Thiol/disulfide homeostasis was evaluated in 30 patients with T1DM and 30 age, gender matched healthy controls. Thiol/disulfide homeostasis parameters were measured using a novel automated measurement method and correlation between demographic data and parameters was measured. RESULTS There weren't any significant differences in age or gender between the T1DM and control groups. T1DM group, findings were as follows: native thiol: 388.3 ± 76.7 µmol/L, total thiol: 426.2 ± 87 µmol/L, disulfide: 18.9 ± 7 µmol/L, control group findings were as follows: native thiol: 423.1 ± 45.2 µmol/L, total thiol: 455.7 ± 49.9 µmol/L, disulfide: 16.2 ± 5.6 µmol/L. The disulfide/native thiol and disulfide/total thiol ratios were significantly higher in the T1DM group (p = 0.005 and p = 0.004, respectively), whereas the native thiol level and the native thiol/total thiol ratio were significantly lower in the T1DM group than in the control group (p = 0.036 and p = 0.015, respectively). There wasn't significant correlation between demographic data and thiol/disulfide homeostasis parameters. DISCUSSION This study shows that dynamic thiol/disulfide homeostasis in children with T1DM shifts towards the disulfide direction. We think that this shift is caused by oxidative damage in β-cells. Additional research on thiol/disulfide homeostasis in children with T1DM might provide techniques for early detection of oxidative damage in β-cells.
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Affiliation(s)
- Sevgi Yaşar Durmuş
- Dr. Sami Ulus Obstetrics and Pediatrics Training and Research Hospital, Division of Pediatrics, Ankara, Turkey.
| | - Nursel Muratoğlu Şahin
- Dr. Sami Ulus Obstetrics and Pediatrics Training and Research Hospital, Division of Pediatrics, Department of Pediatric Endocrinology, Ankara, Turkey
| | - Merve Ergin
- Yildirim Beyazit University, Department of Biochemistry, Ankara, Turkey
| | - Salih Neşelioğlu
- Yildirim Beyazit University, Department of Biochemistry, Ankara, Turkey
| | - Zehra Aycan
- Dr. Sami Ulus Obstetrics and Pediatrics Training and Research Hospital, Division of Pediatrics, Department of Pediatric Endocrinology, Ankara, Turkey
| | - Özcan Erel
- Yildirim Beyazit University, Department of Biochemistry, Ankara, Turkey
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Eren MA, Koyuncu İ, İncebıyık H, Karakaş H, Erel Ö, Sabuncu T. The evaluation of thiol/disulphide homeostasis in diabetic nephropathy. Diabetes Res Clin Pract 2019; 148:249-253. [PMID: 30690040 DOI: 10.1016/j.diabres.2019.01.022] [Citation(s) in RCA: 12] [Impact Index Per Article: 2.0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Journal Information] [Submit a Manuscript] [Subscribe] [Scholar Register] [Received: 05/29/2018] [Revised: 12/14/2018] [Accepted: 01/15/2019] [Indexed: 01/25/2023]
Abstract
AIMS Thiol/disulphide homeostasis plays a critical role in antioxidant defense, and detoxification in body. Although alteration of thiol/disulfide homeostasis had been shown in patients with diabetes, the thiol/disulfide balance in patients with type 2 diabetes and nephropathy is not yet known. METHODS Twenty-six healthy volunteers (group 1), and 17 normal albuminuric (group 2), 24 middle albuminuric (group 3), 20 severe proteinuric (group 4) patients with type 2 diabetes were included. Proteinuria was tested by measuring microalbumin/creatinine ratio in spot urine. Thiol/disulphide homeostasis concentrations were measured using method developed by Erel et al. RESULTS: Mean blood urea and creatinine levels were found to be significantly higher and GFR level was found to be significantly lower in group 4 than in the other groups. Native thiol levels are significantly lower in groups with diabetes than in healthy group and in groups 3 and 4, compared to group 2. Total thiol level was significantly lower in groups 3 and 4 than group 1 and 2. Disulphide/native thiol and disulfide/total thiol ratios were significantly higher in the groups with diabetes than in group 1 and in the group 4 than in the group 2. CONCLUSION The level of native and total thiols were found to be decreased significantly with the grade of nephropathy in patients with type 2 diabetes and the balance had been disrupted in favor of disulphide. We suggest that deteriorated thiol/disulphide balance may be one of the important factors in the development or progression of diabetes induced nephropathy.
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Affiliation(s)
- Mehmet Ali Eren
- Harran University, School of Medicine, Department of Endocrinology, Sanliurfa, Turkey.
| | - İsmail Koyuncu
- Harran University, School of Medicine, Department of Medical Biochemistry, Sanliurfa, Turkey
| | - Hatice İncebıyık
- Harran University, School of Medicine, Department of Endocrinology, Sanliurfa, Turkey
| | - Hilal Karakaş
- Harran University, School of Medicine, Department of Internal Medicine, Sanliurfa, Turkey
| | - Özcan Erel
- Yildirim Beyazit University, School of Medicine, Department of Medical Biochemistry, Ankara, Turkey
| | - Tevfik Sabuncu
- Harran University, School of Medicine, Department of Endocrinology, Sanliurfa, Turkey
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Balamir I, Ates I, Topcuoglu C, Turhan T. Association of Endocan, Ischemia-Modified Albumin, and hsCRP Levels With Endothelial Dysfunction in Type 2 Diabetes Mellitus. Angiology 2018; 69:609-616. [PMID: 29172652 DOI: 10.1177/0003319717740781] [Citation(s) in RCA: 37] [Impact Index Per Article: 5.3] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 01/03/2025]
Abstract
We investigated the relationship of ischemia-modified albumin (IMA) and high-sensitivity C-reactive protein (hsCRP) levels with direct (endocan) and indirect (carotid intima-media thickness [cIMT] and 24 hours urine protein excretion) endothelial dysfunction indicators in type 2 diabetes mellitus (T2DM). Patients with T2DM (n = 88) and 88 healthy individuals were included in the study. The median endocan (475.15 vs 216.37 pg/mL; P < .001, respectively) and hsCRP (10.74 vs 3.11 mg/L; P < .001, respectively) and the mean IMA (0.64 ± 0.12 vs 0.51 ± 0.12 absorbance units; P < .001, respectively) levels were higher in participants with endothelial dysfunction compared to those without endothelial dysfunction in T2DM. The 24-hour urine protein excretion and cIMT levels had a positive correlation with hsCRP ( r = .357; P = .001 and r = .592; P < .001, respectively), IMA ( r = .519; P < .001 and r = .495; P < .001, respectively) and endocan ( r = .347; P = .001 and r = .583; P < .001, respectively) levels in the T2DM group. Stepwise multivariable logistic regression analysis, which included laboratory findings found to be associated with endothelial dysfunction, showed that endocan (odds ratio [OR] = 1.456; P = .004), hsCRP (OR = 1.298; P = .008), and IMA (OR = 2.270, P = .003) were independent risk factors. It was found that none of these markers were superior in terms of diagnostic discrimination for endothelial dysfunction. Endocan, IMA, and hsCRP levels were found to be associated with endothelial dysfunction in patients with T2DM.
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Affiliation(s)
- Ilhan Balamir
- 1 Department of Biochemistry, Ankara Numune Training and Research Hospital, Ankara, Turkey
| | - Ihsan Ates
- 2 Department of Internal Medicine, Ankara Numune Training and Research Hospital, Ankara, Turkey
| | - Canan Topcuoglu
- 1 Department of Biochemistry, Ankara Numune Training and Research Hospital, Ankara, Turkey
| | - Turan Turhan
- 1 Department of Biochemistry, Ankara Numune Training and Research Hospital, Ankara, Turkey
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Mertoğlu C, Gunay M, Siranli G, Kulhan M, Gok G, Erel Ö. The Effect of the 50 g Glucose Challenge Test on The Thiol/Disulfide Homeostasis in Pregnancy. Fetal Pediatr Pathol 2018; 37:147-156. [PMID: 29694252 DOI: 10.1080/15513815.2018.1453002] [Citation(s) in RCA: 10] [Impact Index Per Article: 1.4] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Submit a Manuscript] [Subscribe] [Scholar Register] [Indexed: 12/17/2022]
Abstract
AIM A 50 g glucose challenge test (GCT) is recommended for screening all pregnant women for gestational diabetes mellitus. In this study, the effect of GCT on the thiol/disulfide balance was investigated. METHODS One-hundred women that underwent a 50 g GCT at 24-28 weeks of gestation (63 positive and 37 negative results) were evaluated in terms of thiol/disulfide in serum samples at test hours 0 and 1. RESULTS Compared to the baseline values (hour 0), after the glucose load (hour 1), the thiol and native thiol/total thiol (p < 0.0001) of the GCT-positive women were reduced whereas the values of glucose, disulfide, disulfide/native thiol, disulfide/total thiol (p < 0.0001) and total thiol increased (p = 0.018). CONCLUSION In GCT-positive pregnant individuals, the glucose load increases oxidative stress by changing the thiol/disulfide homeostasis. Such an effect is not observed in healthy pregnancies.
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Affiliation(s)
- Cuma Mertoğlu
- a Department of Clinical Biochemistry , Faculty of Medicine, Erzincan University , Erzincan , Turkey
| | - Murat Gunay
- a Department of Clinical Biochemistry , Faculty of Medicine, Erzincan University , Erzincan , Turkey
| | - Gulsah Siranli
- a Department of Clinical Biochemistry , Faculty of Medicine, Erzincan University , Erzincan , Turkey
| | - Mehmet Kulhan
- b Department of Obstetrics and Gynecology , Faculty of Medicine, Erzincan University , Erzincan , Turkey
| | - Gamze Gok
- c Department of Clinical Biochemistry , Faculty of Medicine, Yildirim Beyazit University , Ankara , Turkey
| | - Özcan Erel
- c Department of Clinical Biochemistry , Faculty of Medicine, Yildirim Beyazit University , Ankara , Turkey
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Gulpamuk B, Tekin K, Sonmez K, Inanc M, Neselioglu S, Erel O, Yilmazbas P. The significance of thiol/disulfide homeostasis and ischemia-modified albumin levels to assess the oxidative stress in patients with different stages of diabetes mellitus. Scand J Clin Lab Invest 2018; 78:136-142. [PMID: 29298534 DOI: 10.1080/00365513.2017.1422540] [Citation(s) in RCA: 36] [Impact Index Per Article: 5.1] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 08/02/2017] [Revised: 11/25/2017] [Accepted: 12/17/2017] [Indexed: 02/08/2023]
Abstract
This study investigated the value of Thiol/Disulfide homeostasis and ischemia-modified albumin (IMA) levels in discriminating diabetic cases with different stages of retinopathy and without retinopathy. In total, 122 patients with type 2 diabetes mellitus (DM) were enrolled in this prospective cross-sectional study. These patients were separated into three subgroups: Group 1 included 42 patients with DM and no diabetic retinopathy (DR), Group 2 included 40 patients with DM having non-proliferative DR and the Group 3 had 40 patients with DM having proliferative DR. The native thiol, total thiol, and disulfide levels and disulfide-native thiol, disulfide-total thiol, and native thiol-total thiol ratios as well as the IMA levels were analyzed and compared among the groups. There were no statistically significant differences regarding the ages and genders of the patients between the groups. The native thiol level, the total thiol level and the native thiol-total thiol ratio showed a statistically significantly reduction, while the disulfide level, the disulfide-native thiol ratio, and the disulfide-total thiol ratio showed a statistically significantly elevation in the Group 3 compared with the Group 1 and Group 2. Additionally, the mean IMA levels were statistically significantly higher in Group 3 when compared to Group 1 and Group 2 (p = .003 and p = .014, respectively). In conclusion, both Thiol/Disulfide homeostasis parameters and IMA levels increase with the progression of DR. Thiol/Disuldife homeostasis balance and IMA levels may be used a biomarker to monitor the tissue ischemia in DM and to discriminate the different stages of DR, in the future.
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Affiliation(s)
| | | | - Kenan Sonmez
- c Ulucanlar Eye Training and Research Hospital , Ankara , Turkey
| | - Merve Inanc
- c Ulucanlar Eye Training and Research Hospital , Ankara , Turkey
| | - Salim Neselioglu
- d Clinical Biochemistry Department , Yıldırım Beyazıt University , Ankara , Turkey
| | - Ozcan Erel
- d Clinical Biochemistry Department , Yıldırım Beyazıt University , Ankara , Turkey
| | - Pelin Yilmazbas
- c Ulucanlar Eye Training and Research Hospital , Ankara , Turkey
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Tokgöz H, Taş S, Giray Ö, Yalçınkaya S, Tokgöz Ö, Koca C, Savaş M, Erel Ö. The change in serum Thiol/Disulphide homeostasis after transrectal ultrasound guided prostate biopsy. Int Braz J Urol 2017; 43:455-461. [PMID: 28128906 PMCID: PMC5462135 DOI: 10.1590/s1677-5538.ibju.2016.0114] [Citation(s) in RCA: 6] [Impact Index Per Article: 0.8] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 02/02/2016] [Accepted: 10/30/2016] [Indexed: 12/20/2022] Open
Abstract
Objectives The aim of this prospective clinical study was to investigate variations in a novel oxidative stress marker (thiol/disulphide homeostasis) in men who underwent transrectal ultrasound guided prostate biopsy (TRUSB). Materials and Methods A total of 22 men undergoing TRUSB of the prostate were enrolled in the study. Patients with abnormal digital rectal examination and/or total prostate specific antigen (PSA) over 4ng/mL underwent TRUSB with 12 cores. Serum samples were obtained before and just after the procedure to evaluate the possible changes in thiol/disulphide homeostasis. Mean age, total PSA and free PSA, prostate volume and histopathological data were also recorded. Results Mean age of the study population was 65.05±8.89 years. Significant decreases in native and total thiol levels were documented after the biopsy procedure. However, serum disulphide levels and disulphide/native thiol, disulphide/total thiol and native/total thiol ratios did not significantly change after TRUSB. No correlation was observed between oxidative parameters and total PSA and free PSA levels, prostate volume and histopathology of the prostate. However, mean patient age was significantly correlated with mean native and total thiol levels. Conclusion Significant decreases in serum native and total thiol levels related to the prostate biopsy procedure suggest that TRUSB causes acute oxidative stress in the human body. Since our trial is the first in the current literature to investigate these oxidative stress markers in urology practice, additional studies are warranted.
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Affiliation(s)
- Hüsnü Tokgöz
- Department of Urology, Antalya Training and Research Hospital, Antalya, Turkey
| | - Selim Taş
- Department of Urology, Antalya Training and Research Hospital, Antalya, Turkey
| | - Özlem Giray
- Department of Biochemistry , Antalya Training and Research Hospital, Antalya, Turkey
| | - Soner Yalçınkaya
- Department of Urology, Antalya Training and Research Hospital, Antalya, Turkey
| | - Özlem Tokgöz
- Department of Radiology, Antalya Training and Research Hospital, Antalya, Turkey
| | - Cemile Koca
- Department of Biochemistry, Yildirim Beyazit University, School of Medicine, Ankara, Turkey
| | - Murat Savaş
- Department of Urology, Antalya Training and Research Hospital, Antalya, Turkey
| | - Özcan Erel
- Department of Biochemistry, Yildirim Beyazit University, School of Medicine, Ankara, Turkey
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Omma A, Sandikci SC, Kücüksahin O, Alisik M, Erel O. Can the Thiol/Disulfide Imbalance Be a Predictor of Colchicine Resistance in Familial Mediterranean Fever? J Korean Med Sci 2017; 32:1588-1594. [PMID: 28875601 PMCID: PMC5592171 DOI: 10.3346/jkms.2017.32.10.1588] [Citation(s) in RCA: 16] [Impact Index Per Article: 2.0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Submit a Manuscript] [Subscribe] [Scholar Register] [Received: 04/22/2017] [Accepted: 06/01/2017] [Indexed: 12/28/2022] Open
Abstract
Familial Mediterranean fever (FMF) is a chronic autoinflammatory condition characterized by fever attacks and recurrent polyserositis. Subclinical inflammation that persists during attack-free periods can result in oxidative stress (OS) damage. Thiol groups bind to reactive oxygen radicals and protect cells and tissues from OS damage. The aim of this study was to investigate the relationship between thiol-disulfide balance and colchicine resistance in FMF patients during an attack or attack-free period. A newly developed spectrophotometric method was used to measure native thiol (NT) and disulfide (DS) levels in FMF patients and an age-sex matched group of healthy controls. NT and DS levels were compared in FMF patients 1) with vs. without colchicine resistance; and 2) during an attack (FMF-AP) vs. attack-free period (FMF-AFP). A total of 118 FMF patients and 60 healthy controls were studied. NT (P < 0.001) and total thiol (TT) (P < 0.001) levels in FMF patients were significantly lower compared to healthy controls. NT (P = 0.030) and TT (P = 0.010) levels of FMF-AP patients were significantly lower than that of FMF-AFP patients. FMF-AP patients had significantly higher DS levels than FMF-AFP patients (P = 0.039). Compared to FMF patients without colchicine resistance, elevated levels of DS (P = 0.019) but not NT (P = 0.620) and TT (P = 0.718) were found in those with colchicine resistance. Thiol-disulfide homeostasis is altered in FMF patients during an attack period and this imbalance may be associated with colchicine resistance.
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Affiliation(s)
- Ahmet Omma
- Department of Rheumatology, Ankara Numune Training and Research Hospital, Ankara, Turkey.
| | - Sevinc Can Sandikci
- Department of Rheumatology, Ankara Numune Training and Research Hospital, Ankara, Turkey
| | - Orhan Kücüksahin
- Division of Rheumatology, Department of Internal Medicine, Yildirim Beyazit University, Ankara, Turkey
| | - Murat Alisik
- Department of Clinical Biochemistry, Faculty of Medicine, Yildirim Beyazit University, Ankara, Turkey
| | - Ozcan Erel
- Department of Clinical Biochemistry, Faculty of Medicine, Yildirim Beyazit University, Ankara, Turkey
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Dynamic thiol/disulphide homeostasis as a novel indicator of oxidative stress in obese children and its relationship with inflammatory-cardiovascular markers. Anatol J Cardiol 2017; 18:361-369. [PMID: 28761018 PMCID: PMC5731286 DOI: 10.14744/anatoljcardiol.2017.7740] [Citation(s) in RCA: 15] [Impact Index Per Article: 1.9] [Reference Citation Analysis] [Abstract] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 01/02/2023] Open
Abstract
Objective: Childhood obesity is an important cause of cardiovascular risk with chronic inflammation. Oxidative stress may contribute to the pathogenesis of obesity-related cardiovascular pathologies. We aimed to evaluate thiol/disulphide homeostasis as a novel and sensitive marker of oxidative stress and to evaluate its relationship with some inflammatory and cardiovascular markers in obese children. Methods: In this case-controlled study, 65 children with exogenous obesity and 64 healthy children, as a control group, were included. In both groups, thiol/disulphide homeostasis parameters and inflammatory (white blood cells, platelets, mean corpuscular volume, neutrophil/lymphocyte ratio, and high-sensitivity C-reactive protein) and cardiovascular (epicardial adipose tissue thickness and left ventricular mass index) markers were studied. Correlation analyses of thiol/disulphide homeostasis parameters with body mass index standard deviation scores (BMI SDS) and inflammatory and cardiovascular markers were performed. Receiver-operating characteristic analysis was performed to determine the sensitivity, specificity, and optimal cut-off values of thiol/disulphide homeostasis parameters. Results: Native thiol, total thiol, and native thiol/total thiol ratios (antioxidant parameters) were lower (p<0.05) and disulphide/native thiol and disulphide/total thiol ratios (oxidant parameters) were higher in the obese group than in the control group (p<0.01). A positive correlation of oxidant parameters with BMI SDS and inflammatory markers was found. However, a negative correlation of antioxidant parameters with BMI SDS and inflammatory markers was found. The specificities of disulphide/native thiol and disulphide/total thiol ratios were higher in the obese group. Conclusion: The impairment in thiol/disulphide homeostasis, which is indicative of oxidative stress, is associated with inflammation in obesity. In addition, cardiovascular involvement may also contribute to this impairment.
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Gulpamuk B, Koç M, Karatepe MS, Yildiz A, Erel O, Neselioglu S, Yilmazbas P. Novel Assay Assessment of Oxidative Stress Biomarkers in Patients with Keratoconus: Thiol–Disulfide Homeostasis. Curr Eye Res 2017; 42:1215-1219. [DOI: 10.1080/02713683.2017.1302592] [Citation(s) in RCA: 7] [Impact Index Per Article: 0.9] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 01/19/2023]
Affiliation(s)
- Bayram Gulpamuk
- Department of Opthalmology, Ankara Ulucanlar Eye Education and Research Hospital, Ankara, Turkey
| | - Mustafa Koç
- Department of Opthalmology, Ankara Ulucanlar Eye Education and Research Hospital, Ankara, Turkey
| | - Mustafa Salih Karatepe
- Department of Opthalmology, Ankara Ulucanlar Eye Education and Research Hospital, Ankara, Turkey
| | - Aydan Yildiz
- Clinical Microbiology Department, Ankara Education and Research Hospital, Ankara, Turkey
| | - Ozcan Erel
- Biochemistry Department, Ankara Ataturk Education and Research Hospital, Ankara, Turkey
| | - Salim Neselioglu
- Biochemistry Department, Ankara Ataturk Education and Research Hospital, Ankara, Turkey
| | - Pelin Yilmazbas
- Ophthalmology Department, Ulucanlar Eye Education and Research Hospital, Ankara, Turkey
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Kaplan M, Ates I, Yuksel M, Ozderin Ozin Y, Alisik M, Erel O, Kayacetin E. Thiol/disulphide homeostasis in celiac disease. World J Gastrointest Pharmacol Ther 2017; 8:120-126. [PMID: 28533921 PMCID: PMC5421110 DOI: 10.4292/wjgpt.v8.i2.120] [Citation(s) in RCA: 14] [Impact Index Per Article: 1.8] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Journal Information] [Submit a Manuscript] [Subscribe] [Scholar Register] [Received: 11/30/2016] [Revised: 01/25/2017] [Accepted: 03/12/2017] [Indexed: 02/06/2023] Open
Abstract
AIM To determine dynamic thiol/disulphide homeostasis in celiac disease and to examine the associate with celiac autoantibodies and gluten-free diet. METHODS Seventy three patients with celiac disease and 73 healthy volunteers were enrolled in the study. In both groups, thiol/disulphide homeostasis was examined with a new colorimetric method recently developed by Erel and Neselioglu. RESULTS In patients with celiac disease, native thiol (P = 0.027) and total thiol (P = 0.031) levels were lower, while disulphide (P < 0.001) level, disulphide/native thiol (P < 0.001) and disulphide/total thiol (P < 0.001) ratios were higher compared to the control group. In patients who do not comply with a gluten-free diet, disulphide/native thiol ratio was found higher compared to the patients who comply with the diet (P < 0.001). In patients with any autoantibody-positive, disulphide/native thiol ratio was observed higher compared to the patients with autoantibody-negative (P < 0.05). It is found that there is a negative correlation between celiac autoantibodies, and native thiol, total thiol levels and native thiol/total thiol ratio, while a positive correlation is observed between disulphide, disulphide/native thiol and disulphide/total thiol levels. CONCLUSION This study is first in the literature which found that the patients with celiac disease the dynamic thiol/disulphide balance shifts through disulphide form compared to the control group.
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Koc U, Tan S, Ertem AG, Gumus M, Ozbek B, Erel O. Evaluation of thiol-disulphide homeostasis in radiation workers. Int J Radiat Biol 2017; 93:705-710. [DOI: 10.1080/09553002.2017.1304668] [Citation(s) in RCA: 8] [Impact Index Per Article: 1.0] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 10/20/2022]
Affiliation(s)
- Ural Koc
- Department of Radiology, Erzincan University Mengucek Gazi Training and Research Hospital, Erzincan, Turkey
| | - Sinan Tan
- Department of Radiology, Kırıkkale University, Kırıkkale, Turkey
| | - Ahmet Goktug Ertem
- Section of Cardiology, Ankara Yuksek Ihtisas Training and Research Hospital, Ankara, Turkey
| | - Mehmet Gumus
- Department of Radiology, Yıldırım Beyazıt University, Ankara, Turkey
| | - Betul Ozbek
- Section of Biochemistry, Ankara Ataturk Training and Research Hospital, Ankara, Turkey
| | - Ozcan Erel
- Department of Biochemistry, Yıldırım Beyazıt University, Ankara, Turkey
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Cetin O, Karaman E, Boza B, Cim N, Alisik M, Erel O, Kolusarı A, Sahin HG. The maternal serum thiol/disulfide homeostasis is impaired in pregnancies complicated by idiopathic intrauterine growth restriction. J Matern Fetal Neonatal Med 2017; 31:607-613. [DOI: 10.1080/14767058.2017.1293025] [Citation(s) in RCA: 8] [Impact Index Per Article: 1.0] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 01/22/2023]
Affiliation(s)
- Orkun Cetin
- Department of Obstetrics and Gynecology, Faculty of Medicine, Yuzuncu Yil University, Van, Turkey
| | - Erbil Karaman
- Department of Obstetrics and Gynecology, Faculty of Medicine, Yuzuncu Yil University, Van, Turkey
| | - Baris Boza
- Department of Obstetrics and Gynecology, Faculty of Medicine, Yuzuncu Yil University, Van, Turkey
| | - Numan Cim
- Department of Obstetrics and Gynecology, Faculty of Medicine, Yuzuncu Yil University, Van, Turkey
| | - Murat Alisik
- Department of Biochemistry, Faculty of Medicine, Yıldırım Beyazıt University, Ankara, Turkey
| | - Ozcan Erel
- Department of Biochemistry, Faculty of Medicine, Yıldırım Beyazıt University, Ankara, Turkey
| | - Ali Kolusarı
- Department of Obstetrics and Gynecology, Faculty of Medicine, Yuzuncu Yil University, Van, Turkey
| | - Hanım Guler Sahin
- Department of Obstetrics and Gynecology, Faculty of Medicine, Yuzuncu Yil University, Van, Turkey
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Hanikoglu F, Hanikoglu A, Kucuksayan E, Alisik M, Gocener AA, Erel O, Baykara M, Cuoghi A, Tomasi A, Ozben T. Dynamic thiol/disulphide homeostasis before and after radical prostatectomy in patients with prostate cancer. Free Radic Res 2016; 50:S79-S84. [DOI: 10.1080/10715762.2016.1235787] [Citation(s) in RCA: 31] [Impact Index Per Article: 3.4] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 10/21/2022]
Affiliation(s)
| | - Aysegul Hanikoglu
- Department of Biochemistry, Medical Faculty, Akdeniz University, Antalya, Turkey
| | - Ertan Kucuksayan
- Department of Biochemistry, Medical Faculty, Akdeniz University, Antalya, Turkey
| | - Murat Alisik
- Department of Biochemistry, Medical Faculty, Yildirim Beyazit University, Ankara, Turkey
| | - Ahmet Anil Gocener
- Department of Urology, Medical Faculty, Akdeniz University, Antalya, Turkey
| | - Ozcan Erel
- Department of Biochemistry, Medical Faculty, Yildirim Beyazit University, Ankara, Turkey
| | - Mehmet Baykara
- Department of Urology, Medical Faculty, Akdeniz University, Antalya, Turkey
| | - Aurora Cuoghi
- Department of Diagnostic and Clinical Medicine and Public Health, University of Modena and Reggio Emilia, Modena, Italy
| | - Aldo Tomasi
- Department of Diagnostic and Clinical Medicine and Public Health, University of Modena and Reggio Emilia, Modena, Italy
| | - Tomris Ozben
- Department of Biochemistry, Medical Faculty, Akdeniz University, Antalya, Turkey
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Ergin M, Caliskanturk M, Senat A, Akturk O, Erel O. Disulfide stress in carbon monoxide poisoning. Clin Biochem 2016; 49:1243-1247. [PMID: 27497239 DOI: 10.1016/j.clinbiochem.2016.07.019] [Citation(s) in RCA: 8] [Impact Index Per Article: 0.9] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 03/30/2016] [Revised: 07/25/2016] [Accepted: 07/28/2016] [Indexed: 12/19/2022]
Abstract
OBJECTIVES Carbon monoxide (CO) remains the most common cause of lethal poisoning around the world. The purpose of this study was to investigate the homeostasis between thiol-disulfide couples and to evaluate oxidative status comprehensively in acute CO poisoning, using new parameters along with other well-known oxidant-antioxidant molecules. DESIGN AND METHODS This case study consisted of 43 subjects who were diagnosed with carbon monoxide poisoning and 35 healthy individuals who were used as controls. Thiol-disulfide paired tests were examined in both groups using the method developed recently. RESULTS Patients with CO poisoning had significantly higher levels of serum disulfide than the control patients (20.7±5.03 versus 16.43±3.97, p=0.001). Native thiol and total thiol levels were lower in the CO patient group than in the control group (p<0.001, for each variable). The disulfide/native thiol ratios and disulfide/total thiol ratios were significantly higher, while native thiol/total thiol ratios were significantly lower, in patients with acute CO poisoning than in the healthy controls (p<0.001, for all ratios). The disulfide/native ratios were negatively correlated with both total antioxidant response and paraoxonase and arylesterase values and were positively correlated with total oxidant status and ceruloplasmin values (p<0.05, for all correlations). CONCLUSIONS Excessive disulfide levels and their related ratios were found in CO poisoning patients. In particular, the disulfide/native thiol ratio was identified as an indicator for overall oxidative status. Among CO poisoning patients, the thiol-disulfide balance was found to be impaired. Therefore, the disruption of thiol-disulfide homeostasis might be involved in CO toxicity.
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Affiliation(s)
- Merve Ergin
- Department of Biochemistry, 25 Aralik State Hospital, Gaziantep, Turkey.
| | | | - Almila Senat
- Department of Biochemistry, Yildirim Beyazit University, Faculty of Medicine, Ankara, Turkey
| | - Onur Akturk
- Department of Biochemistry, 25 Aralik State Hospital, Gaziantep, Turkey
| | - Ozcan Erel
- Department of Biochemistry, Yildirim Beyazit University, Faculty of Medicine, Ankara, Turkey
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Kızıltunç E, Gök M, Kundi H, Çetin M, Topçuoğlu C, Gülkan B, Çiçekçioğlu H, Örnek E. Plasma thiols and thiol-disulfide homeostasis in patients with isolated coronary artery ectasia. Atherosclerosis 2016; 253:209-213. [PMID: 27451003 DOI: 10.1016/j.atherosclerosis.2016.07.904] [Citation(s) in RCA: 21] [Impact Index Per Article: 2.3] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Submit a Manuscript] [Subscribe] [Scholar Register] [Received: 02/06/2016] [Revised: 07/14/2016] [Accepted: 07/14/2016] [Indexed: 12/25/2022]
Abstract
BACKGROUND AND AIMS Thiol/disulfide homeostasis has an important role in the antioxidant defense system. Oxidative stress may contribute to the pathogenesis of coronary artery ectasia. The aim of this study was to evaluate plasma thiol levels and thiol/disulfide homeostasis in patients with isolated coronary artery ectasia. METHODS Forty-one patients with isolated coronary artery ectasia and 72 patients with normal coronary arteries were included in the study. Markis classification and number of ectatic coronary arteries were recorded. Plasma total thiol levels, native thiol levels and disulfide levels were measured. Thiol/disulfide homeostasis was appraised by calculating thiol/disulfide ratio. RESULTS Plasma native thiol levels were significantly lower (336.9 (252.9-374.1) vs. 353.1 (327.0-380.0), p = 0.041) and disulfide levels were significantly higher (18.9 ± 6.3 vs. 16.6 ± 3.4, p = 0.014) in patients with coronary artery ectasia than control patients. Both native thiol/disulfide and total thiol/disulfide ratio was significantly lower in the coronary artery ectasia group (p < 0.001). Multivariate logistic regression analysis revealed that native thiol levels, disulfide levels and native thiol/disulfide ratio were independently associated with the presence of coronary artery ectasia. Thiol/disulfide ratio was not different according to number of ectatic coronary arteries and there was no association between thiol/disulfide ratio and Markis classification. CONCLUSIONS Plasma thiol/disulfide homeostasis is altered in patients with coronary artery ectasia.
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Affiliation(s)
- Emrullah Kızıltunç
- Numune Education and Research Hospital Cardiology Department, Ankara, Turkey.
| | - Murat Gök
- Numune Education and Research Hospital Cardiology Department, Ankara, Turkey
| | - Harun Kundi
- Numune Education and Research Hospital Cardiology Department, Ankara, Turkey
| | - Mustafa Çetin
- Numune Education and Research Hospital Cardiology Department, Ankara, Turkey
| | - Canan Topçuoğlu
- Numune Education and Research Hospital Cardiology Department, Ankara, Turkey
| | - Birsen Gülkan
- Numune Education and Research Hospital Cardiology Department, Ankara, Turkey
| | - Hülya Çiçekçioğlu
- Numune Education and Research Hospital Cardiology Department, Ankara, Turkey
| | - Ender Örnek
- Numune Education and Research Hospital Cardiology Department, Ankara, Turkey
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Dogru A, Balkarli A, Cetin GY, Neselioglu S, Erel O, Tunc SE, Sahin M. Thiol/disulfide homeostasis in patients with ankylosing spondylitis. Bosn J Basic Med Sci 2016; 16:187-92. [PMID: 27186972 DOI: 10.17305/bjbms.2016.1001] [Citation(s) in RCA: 30] [Impact Index Per Article: 3.3] [Reference Citation Analysis] [Abstract] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 01/13/2016] [Revised: 02/22/2016] [Accepted: 02/24/2016] [Indexed: 02/07/2023] Open
Abstract
Ankylosing spondylitis (AS) is a chronic inflammatory disease. In many inflammatory diseases, increased production of pro-inflammatory cytokines is associated with an increase in oxidative stress mediators. Thiol/disulfide homeostasis is a marker for oxidative stress. The aim of this study was to examine the dynamic thiol/disulfide homeostasis in AS. Sixty-nine patients with AS and 60 age- and sex-matched controls were included in the study. The Bath Ankylosing Spondylitis Disease Activity Index (BASDAI) and visual analogue scale (VAS) were used to determine the disease activity. Native thiol, total thiol, and disulfide levels were measured with a novel automated method recently described by Erel and Neselioglu. The aforementioned method is also optionally manual spectrophotometric assay. The total thiol levels were significantly lower in the AS group compared with the control group (p = 0.03). When the patients were divided into active (n = 35) and inactive (n = 34) subgroups using BASDAI scores, the native plasma thiol and total thiol levels were significantly lower in the active AS patients compared to the inactive AS patients (p = 0.02, p = 0.03 respectively). There was a negative correlation between the plasma native thiol levels and VAS, BASDAI scores. Thiol/disulfide homeostasis may be used for elucidating the effects of oxidative stress in AS. Understanding the role of thiol/disulfide homeostasis in AS might provide new therapeutic intervention strategies for patients.
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Affiliation(s)
- Atalay Dogru
- Faculty of Medicine, Suleyman Demirel University.
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Ozler S, Oztas E, Caglar AT, Uygur D, Ergin M, Erel O, Danisman N. Thiol/disulfide homeostasis in predicting adverse perinatal outcomes at 24-28 weeks of pregnancy in gestational diabetes. J Matern Fetal Neonatal Med 2016; 29:3699-704. [PMID: 26769102 DOI: 10.3109/14767058.2016.1141885] [Citation(s) in RCA: 17] [Impact Index Per Article: 1.9] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 12/16/2022]
Abstract
OBJECTIVE The main aim of this study was to investigate thiol/disulfide homeostasis at 24-28 weeks of pregnancy and to evaluate whether it is predictive for adverse perinatal outcomes or not in gestational diabetes mellitus (GDM). METHODS A total of 110 pregnant women at 24-28 weeks of pregnancy (74 GDM patients and 36 age- and BMI-matched healthy pregnant women) were enrolled in this prospective case-control study. Thiol/disulfide homeostasis was evaluated with a novel spectrophotometric method to determine if there is an association with adverse perinatal outcomes in GDM, by using logistic regression analysis. RESULTS GDM patients, with decreased native thiol levels at 24-28 weeks (OR: 4.890, 95% CI: 1.355-5.764, p = 0.015) and with higher pre-pregnancy BMI (OR: 1.280, 95% CI: 1.072-1.528, p = 0.006), were found to be at increased risk of adverse perinatal outcomes in GDM. There were no statistically significant differences in thiol/disulfide homeostasis between diet- and insulin-treated GDM subgroups. Additionally, 1-h and 2-h glucose levels on 100 g OGTT were found to be predictive for the insulin need in achieving good glycemic control in GDM (OR: 1.022, 95% CI: 1.005-1.038, p = 0.010 and OR: 1.019, 95% CI: 1.004-1.035, p = 0.015). CONCLUSIONS GDM patients, with decreased native thiol levels at 24-28 weeks of pregnancy and with higher pre-pregnancy BMI, have an increased risk of possible adverse perinatal outcomes. Also, increased 1-h and 2-h glucose levels on 100 g OGTT can predict the need for insulin treatment for GDM.
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Affiliation(s)
- Sibel Ozler
- a Department of Perinatology , Zekai Tahir Burak Women's Health Training and Research Hospital , Ankara , Turkey and
| | - Efser Oztas
- a Department of Perinatology , Zekai Tahir Burak Women's Health Training and Research Hospital , Ankara , Turkey and
| | - Ali Turhan Caglar
- a Department of Perinatology , Zekai Tahir Burak Women's Health Training and Research Hospital , Ankara , Turkey and
| | - Dilek Uygur
- a Department of Perinatology , Zekai Tahir Burak Women's Health Training and Research Hospital , Ankara , Turkey and
| | - Merve Ergin
- b Department of Clinical Biochemistry , Faculty of Medicine, Yildirim Beyazit University , Ankara , Turkey
| | - Ozcan Erel
- b Department of Clinical Biochemistry , Faculty of Medicine, Yildirim Beyazit University , Ankara , Turkey
| | - Nuri Danisman
- a Department of Perinatology , Zekai Tahir Burak Women's Health Training and Research Hospital , Ankara , Turkey and
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Ates I, Kaplan M, Yuksel M, Mese D, Alisik M, Erel Ö, Yilmaz N, Guler S. Determination of thiol/disulphide homeostasis in type 1 diabetes mellitus and the factors associated with thiol oxidation. Endocrine 2016; 51:47-51. [PMID: 26547218 DOI: 10.1007/s12020-015-0784-6] [Citation(s) in RCA: 86] [Impact Index Per Article: 9.6] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Journal Information] [Submit a Manuscript] [Subscribe] [Scholar Register] [Received: 08/31/2015] [Accepted: 10/20/2015] [Indexed: 01/04/2023]
Abstract
In this study, we aimed to examine dynamic thiol/disulfide homeostasis in type 1 diabetes mellitus (T1DM) and identify the factors associated with thiol oxidation. Thirty-eight subjects (18 male, 20 female) diagnosed with T1DM and 38 (17 male, 21 female) healthy volunteers without any known diseases were included in the study. Thiol/disulfide homeostasis concentrations were measured by a newly developed method (Erel & Neselioglu) in this study. After native thiol, total thiol and disulfide levels were determined; measures such as disulfide/native thiol, disulfide/total thiol, and native thiol/total thiol were calculated. In T1DM patients, compared to the control group, disulfide (p = 0.024), disulfide/native thiol (p < 0.001), and disulfide/total thiol (p < 0.001) were determined higher, while native thiol (p = 0.004) and total thiol (p < 0.001) levels were much lower. In the patient group, a positive correlation was determined between c-reactive protein (r = 325, p = 0.007; r = 316, p = 0.010, respectively), fasting blood glucose (r = 279, p = 0.018; r = 251, p = 0.035, respectively), and glycosylated hemoglobin (r = 341, p = 0.004; r = 332, p = 0.005, respectively) and rates of disulfide/native thiol and disulfide/total thiol. We determined that thiol oxidation increase in T1DM patients compared to the control group. We thought that hyperglycemia and chronic inflammation might be the major cause of increase in oxide thiol form. In order to determine the relationship between the status of autoimmunity and dynamic thiol/disulfide in T1DM, dynamic thiol/disulfide homeostasis in newly diagnosed-antibody positive-T1DM patients is required to be investigated.
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Affiliation(s)
- Ihsan Ates
- Department of Internal Medicine, Ankara Numune Training and Research Hospital, 06100, Ankara, Turkey.
| | - Mustafa Kaplan
- Department of Internal Medicine, Ankara Numune Training and Research Hospital, 06100, Ankara, Turkey
| | - Mahmut Yuksel
- Department of Gastroenterology, Turkiye Yuksek Ihtisas Training and Research Hospital, Ankara, Turkey
| | - Duygu Mese
- Department of Internal Medicine, Ankara Numune Training and Research Hospital, 06100, Ankara, Turkey
| | - Murat Alisik
- Department of Biochemistry, Faculty of Medicine, Yıldırım Beyazit University, Ankara, Turkey
| | - Özcan Erel
- Department of Biochemistry, Faculty of Medicine, Yıldırım Beyazit University, Ankara, Turkey
| | - Nisbet Yilmaz
- Department of Internal Medicine, Ankara Numune Training and Research Hospital, 06100, Ankara, Turkey
| | - Serdar Guler
- Department of Endocrinology, Ankara Numune Training and Research Hospital, Ankara, Turkey
- Department of Endocrinology, School of Medicine, Hitit University, Çorum, Turkey
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