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Yang X, Dai C, Zheng G, Ding S, Wu J, Zhou Q, Zhang A, Sun J. Bisphenol analogues in soils and lettuce (Lactuca sativa L.) around typical factories in eastern China: Occurrence, contamination characteristics, and health risks. ENVIRONMENTAL POLLUTION (BARKING, ESSEX : 1987) 2025; 373:126126. [PMID: 40154866 DOI: 10.1016/j.envpol.2025.126126] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Subscribe] [Scholar Register] [Received: 11/26/2024] [Revised: 02/24/2025] [Accepted: 03/18/2025] [Indexed: 04/01/2025]
Abstract
Following the restrictions on bisphenol A (BPA), the production and environmental release of bisphenol analogues (BPs) have increased. However, knowledge about the occurrence of bisphenol analogues other than BPA, especially in farmland soils and edible plants, remains limited. This study investigated the occurrence, contamination characteristics, and human health risks of eight bisphenol analogues in paired soil-plant samples from areas near factories in eastern China. Results indicated that the concentrations of Σ8BPs in the collected soil and plant samples ranged from 1.4 to 897.1 ng/g dw and 2.5 to 586.2 ng/g dw, respectively. BPA, bisphenol AF (BPAF), bisphenol F (BPF), and bisphenol S (BPS) were the primary components of BPs, with BPA having the highest detection frequency (74%). In addition, a positive correlation was observed between the root concentration factor and the log Kow of BPs (R2 = 0.471, P < 0.05), whereas the translocation factor exhibited a negative correlation with the log Kow (R2 = 0.405, P < 0.05). The hazard index (HI) values of BPs in paired soil-plant samples were <1, suggesting that the current contamination levels of BPs in soils and plants are unlikely to pose significant health risks to humans. However, potential risks from long-term exposure require careful monitoring. This study offers new insights into the spatial distributions and contamination status of BPs in farmland soils and plants, highlighting the environmental behavior and health risks of other bisphenol analogues.
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Affiliation(s)
- Xindong Yang
- Key Laboratory of Microbial Control Technology for Industrial Pollution in Zhejiang Province, College of Environment, Zhejiang University of Technology, Hangzhou 310014, China
| | - Chenan Dai
- Key Laboratory of Microbial Control Technology for Industrial Pollution in Zhejiang Province, College of Environment, Zhejiang University of Technology, Hangzhou 310014, China
| | - Guoxin Zheng
- Key Laboratory of Microbial Control Technology for Industrial Pollution in Zhejiang Province, College of Environment, Zhejiang University of Technology, Hangzhou 310014, China
| | - Shaojie Ding
- International Joint Research Center for Persistent Toxic Substances (IJRC-PTS), College of Environment, Zhejiang University of Technology, Hangzhou 310014, China
| | - Juan Wu
- Key Laboratory of Microbial Control Technology for Industrial Pollution in Zhejiang Province, College of Environment, Zhejiang University of Technology, Hangzhou 310014, China
| | - Qinghua Zhou
- International Joint Research Center for Persistent Toxic Substances (IJRC-PTS), College of Environment, Zhejiang University of Technology, Hangzhou 310014, China
| | - Anping Zhang
- International Joint Research Center for Persistent Toxic Substances (IJRC-PTS), College of Environment, Zhejiang University of Technology, Hangzhou 310014, China
| | - Jianqiang Sun
- Key Laboratory of Microbial Control Technology for Industrial Pollution in Zhejiang Province, College of Environment, Zhejiang University of Technology, Hangzhou 310014, China.
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Ahmad S, Akmal H, Jabeen F, Shahzad K. Exposure to bisphenol S induces organ toxicity by disrupting oxidative and antioxidant defense system and blood physiology in Labeo rohita (Hamilton, 1822). FISH PHYSIOLOGY AND BIOCHEMISTRY 2025; 51:52. [PMID: 39982559 DOI: 10.1007/s10695-025-01467-1] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Subscribe] [Scholar Register] [Received: 07/25/2024] [Accepted: 02/04/2025] [Indexed: 02/22/2025]
Abstract
Bisphenol S is an emerging pollutant that is contaminating aquatic ecosystems and causing detrimental effects on aquatic organisms, especially fish. Therefore, the study was designed to evaluate the toxicity of bisphenol S (BPS) through genotoxic, biochemical, histopathological, and oxidative damage in the liver, gills, and kidneys of Labeo rohita fish. Fish were exposed to three different concentrations (400 µg/L, 800 µg/L, and 1000 µg/L) of BPS for 21 days. A significant (p ≤ 0.05) decline in antioxidant enzymatic activity of superoxide dismutase (SOD), catalase (CAT), reduced glutathione (GSH), and peroxidase (POD) was observed in all tissues, whereas elevation in oxidative contents (TBARS and ROS) was observed. Comet analysis showed elevated olive tail moment and % of DNA damage. Light microscopy revealed several anomalies including cluster nuclei formation, damaged parenchyma cells, sinusoidal spaces, and melanomacrophage in the kidney, sinusoidal spaces, dilated hepatic vein, pyknotic nuclei, melanomacrophage, and cell necrosis in the liver and bone cell deformities, lamellar aneurysm, hyperplasia, and curved secondary gill lamellae in gills. Results of hematobiochemical analysis revealed a significant (p ≤ 0.05) increment in hematocrit, WBCs, cholesterol, blood glucose, triglycerides, AST, ALT, T3, TSH, T4, urea, and creatinine, whereas decline in RBCs, MCH, hemoglobin, proteins levels was observed. The results of the current study demonstrate that BPS has detrimental effects on the kidneys, gills, and liver. It interferes with normal functioning by inhibiting enzymatic activity, causing DNA damage, and disrupting the normal structure of vital organs. These effects make BPS toxic to fish, even at low concentrations.
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Affiliation(s)
- Shabbir Ahmad
- Department of Zoology, University of Okara, Okara, 56130, Pakistan
| | - Hasnain Akmal
- Department of Zoology, University of Okara, Okara, 56130, Pakistan
| | - Farhat Jabeen
- Department of Zoology, Government College University Faisalabad, Faisalabad, 37251, Pakistan
| | - Khurram Shahzad
- Department of Zoology, University of Okara, Okara, 56130, Pakistan.
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Jiang J, Han D, Xiao Y, Song X. Occurrence, migration, and assessment of human health and ecological risks of PFASs and EDCs in groundwater of Northeast China. WATER RESEARCH 2025; 269:122810. [PMID: 39626447 DOI: 10.1016/j.watres.2024.122810] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Subscribe] [Scholar Register] [Received: 08/20/2024] [Revised: 10/24/2024] [Accepted: 11/16/2024] [Indexed: 12/11/2024]
Abstract
Northeast China as an important base of grain production in China, has been suffering from potential groundwater pollution due to the excessive and prolonged application of fertilizers and pesticides. However, exploration of emerging contaminants pollution in groundwater and assessment of human health and ecological risks caused by large-scale agricultural activities have been relatively scarce. This study collected groundwater samples from typical agricultural areas in Northeast China to investigate the extent of contamination by nitrate, per- and polyfluoroalkyl substances (PFASs) and endocrine-disrupting compounds (EDCs), and then compared the levels of these pollutants with those in other regions of China. Groundwater nitrate pollution caused by strong agricultural activity is widespread in Northeast China, with nitrate-nitrogen (NO3N) concentrations exceeding 10 mg/L in as many as 40.3 % of 429 samples. 8 types of PFASs (3.7-7.1 ng/L) and 11 types of EDCs (18,114.0-62,029.8 ng/L) were detected in the collected groundwater samples. Using the Risk Quotient (RQ) method, this study assessed ecological risk and found that the risk level of perfluorooctane sulfonate (PFOS) was higher than that of other PFASs. The groundwater EDCs risks in Northeast China was higher compared to other regions in China, with dibutyl phthalate (DBP), Di-(2-ethylhexyl) phthalate (DEHP), Bisphenol A (BPA) having high ecological risk levels. Nitrate, PFASs and EDCs have been detected in deep groundwater (70-100 m depth), indicating that the deeper aquifers could be significantly threatened by pollutants due to human activities. Fertilizers, pesticides, domestic wastewater, and industrial discharges are major sources of groundwater pollutants in the agricultural regions. Industrial-sourced EDCs were widely detected in groundwater of agricultural area, suggesting that the transport of these pollutants is very active in groundwater system. Groundwater monitoring and pollution prevention are extremely urgent, especially for emerging contaminants. This study can provide important warnings and water resource management references for other agricultural areas affected by intensively agricultural activities in the world.
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Affiliation(s)
- Jiaxun Jiang
- Key Laboratory of Water Cycle & Related Land Surface Processes, Institute of Geographic Sciences and Natural Resources Research, Chinese Academy of Sciences, Beijing 100101, China; College of Resources and Environment, University of Chinese Academy of Sciences, Beijing 100049, China
| | - Dongmei Han
- Key Laboratory of Water Cycle & Related Land Surface Processes, Institute of Geographic Sciences and Natural Resources Research, Chinese Academy of Sciences, Beijing 100101, China; College of Resources and Environment, University of Chinese Academy of Sciences, Beijing 100049, China.
| | - Yi Xiao
- Key Laboratory of Water Cycle & Related Land Surface Processes, Institute of Geographic Sciences and Natural Resources Research, Chinese Academy of Sciences, Beijing 100101, China; College of Resources and Environment, University of Chinese Academy of Sciences, Beijing 100049, China
| | - Xianfang Song
- Key Laboratory of Water Cycle & Related Land Surface Processes, Institute of Geographic Sciences and Natural Resources Research, Chinese Academy of Sciences, Beijing 100101, China; College of Resources and Environment, University of Chinese Academy of Sciences, Beijing 100049, China
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Zhang JM, Han H, Fu B, Li YC, Li K, Liu JW, Yu EM, Liu LP. Identification of potential geosmin-binding proteins in grass carp gill based on affinity responsive target stability and tandem mass tag proteomics. ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY 2025; 291:117832. [PMID: 39904256 DOI: 10.1016/j.ecoenv.2025.117832] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Subscribe] [Scholar Register] [Received: 11/07/2024] [Revised: 01/28/2025] [Accepted: 01/30/2025] [Indexed: 02/06/2025]
Abstract
The escalating issue of water pollution, especially the accumulation of organic off-flavor pollutants, poses significant challenges. Geosmin, a typical off-flavor compound in aquatic environments, not only compromises the quality of aquatic products but also deters consumers. Its impact extends to aquatic organisms, with current research focusing on dose-response and ecotoxicity, while neglecting the molecular-level study of geosmin-binding proteins. This study employs an integrated approach combing affinity-responsive target stability in vitro, tandem mass tag proteomics in vivo, and molecular docking to identify geosmin-binding proteins in the gill tissue of grass carp (Ctenopharyngodon idella). ARTS analysis identified 56 proteins, predominantly membrane-associated proteins, such as catenin beta-1, annexin, and integrin beta. Proteomic analysis revealed 256 differentially expressed proteins in geosmin-exposure group, with 18 common proteins screened by in vivo and in vitro methods. Among these, annexin, cathepsin D, and interleukin-1 receptors were highlighted as potential geosmin targets, with annexin demonstrating the highest binding affinity in silico. This study provides a robust protocol integrating in vivo, in vitro, and in silico approaches to elucidate geosmin's target proteins in grass carp gill tissue, advancing our understanding of pollutant-biological interactions and enhancing environmental risk assessment accuracy.
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Affiliation(s)
- Jun-Ming Zhang
- China-ASEAN Belt and Road Joint Laboratory on Mariculture Technology (Shanghai), Shanghai Ocean University, Shanghai 201306, China
| | - Huan Han
- China-ASEAN Belt and Road Joint Laboratory on Mariculture Technology (Shanghai), Shanghai Ocean University, Shanghai 201306, China
| | - Bing Fu
- College of Marine Sciences, South China Agricultural University, Guangzhou 510640, China
| | - Yi-Chao Li
- Guangxi Academy of Marine Sciences, Nanning, 530000, China
| | - Kang Li
- China-ASEAN Belt and Road Joint Laboratory on Mariculture Technology (Shanghai), Shanghai Ocean University, Shanghai 201306, China; Center for Ecological Aquaculture (CEA), Shanghai Ocean University, Shanghai 201306, China.
| | - Jing-Wei Liu
- China-ASEAN Belt and Road Joint Laboratory on Mariculture Technology (Shanghai), Shanghai Ocean University, Shanghai 201306, China; Shanghai Engineering Research Center of Aquaculture, Shanghai Ocean University, Shanghai 201306, China
| | - Er-Meng Yu
- Guangxi Academy of Marine Sciences, Nanning, 530000, China
| | - Li-Ping Liu
- China-ASEAN Belt and Road Joint Laboratory on Mariculture Technology (Shanghai), Shanghai Ocean University, Shanghai 201306, China; Shanghai Engineering Research Center of Aquaculture, Shanghai Ocean University, Shanghai 201306, China.
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Mit C, Beaudouin R, Palluel O, Turiès C, Daniele G, Giroud B, Bado-Nilles A. Exposure and hazard of bisphenol A, S and F: a multi-biomarker approach in three-spined stickleback. ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH INTERNATIONAL 2025; 32:3343-3356. [PMID: 37436621 DOI: 10.1007/s11356-023-28462-4] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Subscribe] [Scholar Register] [Received: 03/21/2023] [Accepted: 06/23/2023] [Indexed: 07/13/2023]
Abstract
Due to the estrogenic behavior of bisphenol (BP) A, industries have developed many substitutes, such as BPS and BPF. However, due to their structural similarities, adverse effects on reproduction are currently observed in various organisms, including fish. Even if new results have shown impacts of these bisphenols on many other physiological functions, their mode of action remains unclear. In this context, we proposed to better understand the impact of BPA, BPS, and BPF on immune responses (leucocyte sub-populations, cell death, respiratory burst, lysosomal presence, and phagocytic activity) and on biomarkers of metabolic detoxification (ethoxyresorufin-O-deethylase, EROD, and glutathione S-transferase, GST) and oxidative stress (glutathione peroxidase, GPx, and lipid peroxidation with thiobarbituric acid reactive substance method, TBARS) in an adult sentinel fish species, the three-spined stickleback. In order to enhance our understanding of how biomarkers change over time, it is essential to determine the internal concentration responsible for the observed responses. Therefore, it is necessary to explore the toxicokinetics of bisphenols. Thus, sticklebacks were exposed either to 100 μg/L of BPA, BPF or BPS for 21 days, or for seven days to 10 and 100 μg/L of BPA or BPS followed by seven days of depuration. Although BPS has very different TK, due to its lower bioaccumulation compared to BPA and BPF, BPS affect oxidative stress and phagocytic activity in the same way. For those reasons, the replacement of BPA by any substitute should be made carefully in terms of risk assessment on aquatic ecosystems.
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Affiliation(s)
- Corentin Mit
- Experimental Toxicology and Modelling Unit, INERIS, UMR-I 02 SEBIO, 65550, Verneuil en Halatte, France
- Ecotoxicology of Substances and Fields Unit, INERIS, UMR-I 02 SEBIO, 65550, Verneuil en Halatte, France
| | - Rémy Beaudouin
- Experimental Toxicology and Modelling Unit, INERIS, UMR-I 02 SEBIO, 65550, Verneuil en Halatte, France
| | - Olivier Palluel
- Ecotoxicology of Substances and Fields Unit, INERIS, UMR-I 02 SEBIO, 65550, Verneuil en Halatte, France
| | - Cyril Turiès
- Ecotoxicology of Substances and Fields Unit, INERIS, UMR-I 02 SEBIO, 65550, Verneuil en Halatte, France
| | - Gaëlle Daniele
- CNRS, Université Claude Bernard Lyon 1, Institut des Sciences Analytiques, UMR 5280, 5 rue de la Doua, F-69100, Villeurbanne, France
| | - Barbara Giroud
- CNRS, Université Claude Bernard Lyon 1, Institut des Sciences Analytiques, UMR 5280, 5 rue de la Doua, F-69100, Villeurbanne, France
| | - Anne Bado-Nilles
- Ecotoxicology of Substances and Fields Unit, INERIS, UMR-I 02 SEBIO, 65550, Verneuil en Halatte, France.
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Cao X, Qin R, Zhang S, Luo W, Qin J, Yan X, Cai F, Liao Q, Yu Y, Zheng J. Bisphenol pollutants bind with human hair keratin: Combining evidence from fluorescence spectroscopy and molecular docking. THE SCIENCE OF THE TOTAL ENVIRONMENT 2024; 957:177533. [PMID: 39542267 DOI: 10.1016/j.scitotenv.2024.177533] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Subscribe] [Scholar Register] [Received: 09/07/2024] [Revised: 11/06/2024] [Accepted: 11/11/2024] [Indexed: 11/17/2024]
Abstract
Bisphenols, including bisphenol A (BPA) and its analogs such as bisphenol F (BPF), bisphenol S (BPS), tetrabromobisphenol A (TBBPA), tetrachlorobisphenol A (TCBPA) and tetrabromobisphenol S (TBBPS), are typical endocrine disruptors widely used in plastic production. However, until now, the occurrence mechanisms of these bisphenols in hair, a non-invasive material for human biomonitoring, have been inadequately explored. This study employed fluorescence spectroscopy and molecular docking to investigate the interactions between these 6 bisphenols and hair keratin. The findings revealed that these bisphenols quenched keratin's intrinsic fluorescence in a concentration-dependent manner and exhibited a mixed quenching mechanism. Their binding constants to keratin at 308 K range from 6.98 × 102 to 7.24 × 106 M-1, with a spontaneous binding mode observed. Halogenated bisphenols demonstrated a higher binding affinity to keratin compared to non-halogenated bisphenols, with bromobisphenols showing a greater affinity than chlorinated bisphenols. The combined results from fluorescence and molecular docking suggest that hydrogen bonding and hydrophobic interactions are the predominant forces driving the binding of bisphenols to hair keratin. These insights first provide a novel perspective on understanding the mechanisms of small molecular pollutants deposition in hair, marking an important step toward utilizing hair as a biomonitoring tool.
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Affiliation(s)
- Xue Cao
- The Key Laboratory of Environmental Pollution Health Risk Assessment, Research Center of Emerging Contaminants, South China Institute of Environmental Sciences, Ministry of Ecology and Environment, Guangzhou 510655, PR China; Department of Toxicology, School of Public Health, Guangxi Medical University, Nanning, 530021, PR China
| | - Ruixin Qin
- The Key Laboratory of Environmental Pollution Health Risk Assessment, Research Center of Emerging Contaminants, South China Institute of Environmental Sciences, Ministry of Ecology and Environment, Guangzhou 510655, PR China
| | - Shiyi Zhang
- The Key Laboratory of Environmental Pollution Health Risk Assessment, Research Center of Emerging Contaminants, South China Institute of Environmental Sciences, Ministry of Ecology and Environment, Guangzhou 510655, PR China.
| | - Weikeng Luo
- The Key Laboratory of Environmental Pollution Health Risk Assessment, Research Center of Emerging Contaminants, South China Institute of Environmental Sciences, Ministry of Ecology and Environment, Guangzhou 510655, PR China
| | - Jiaxiang Qin
- Kingfa Sci. & Tech. Co., LTD., Guangzhou 510663, PR China
| | - Xiao Yan
- The Key Laboratory of Environmental Pollution Health Risk Assessment, Research Center of Emerging Contaminants, South China Institute of Environmental Sciences, Ministry of Ecology and Environment, Guangzhou 510655, PR China
| | - Fengshan Cai
- The Key Laboratory of Environmental Pollution Health Risk Assessment, Research Center of Emerging Contaminants, South China Institute of Environmental Sciences, Ministry of Ecology and Environment, Guangzhou 510655, PR China
| | - Qilong Liao
- The Key Laboratory of Environmental Pollution Health Risk Assessment, Research Center of Emerging Contaminants, South China Institute of Environmental Sciences, Ministry of Ecology and Environment, Guangzhou 510655, PR China
| | - Yunjiang Yu
- The Key Laboratory of Environmental Pollution Health Risk Assessment, Research Center of Emerging Contaminants, South China Institute of Environmental Sciences, Ministry of Ecology and Environment, Guangzhou 510655, PR China
| | - Jing Zheng
- The Key Laboratory of Environmental Pollution Health Risk Assessment, Research Center of Emerging Contaminants, South China Institute of Environmental Sciences, Ministry of Ecology and Environment, Guangzhou 510655, PR China.
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Khan IA, Yu T, Li Y, Hu C, Zhao X, Wei Q, Zhong Y, Yang M, Liu J, Chen Z. In vivo toxicity of upconversion nanoparticles (NaYF 4:Yb, Er) in zebrafish during early life stages: Developmental toxicity, gut-microbiome disruption, and proinflammatory effects. ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY 2024; 284:116905. [PMID: 39191133 DOI: 10.1016/j.ecoenv.2024.116905] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Subscribe] [Scholar Register] [Received: 06/05/2024] [Revised: 08/09/2024] [Accepted: 08/16/2024] [Indexed: 08/29/2024]
Abstract
Lanthanide-doped upconversion nanoparticles (Ln-UCNPs) have been considered promising materials for various fields, such as biomedical and industrial applications. However, data and reports regarding its toxicity and environmental risks are scarce. Under these circumstances, data must be obtained to fully understand potential toxicity and adverse outcome pathways. In the present study, the toxicity of uncoated Ln-UCNP cores (NaYF4:Yb, Er) was systematically assessed in zebrafish embryos during early developmental stages. Ln-UCNPs were found to have multiple toxic effects, such as effects on survival rates, delayed hatching times, shorter body lengths, altered heart rates and blood circulation (significantly reduced), and neurobehavioral impairments in response to photoperiod stimulation. Bioimaging showed that Ln-UCNPs were distributed on the chorion, eyes, and skin at 72 hpf. However, it accumulates in the pharynx, esophagus, and intestine after oral administration. Ln-UCNPs disrupt the diversity and abundance of host-associated microorganisms (gut microbiota) leading to an increase in the prevalence of harmful bacteria in zebrafish. Transcriptomic and Ingenuity Pathway Analysis (IPA) predicted Interleukin-8 (IL-8) signaling, neuroinflammation, cardiac hypertrophy signaling pathways, immune and inflammation-related response interferon-gamma (ifnγ), and miR-155 as key mediators in regulatory effects. Based on this, a causal network was built showing the strong links between the induced gene expression of differentially expressed genes (DEGs), such as nitric oxide synthase 2 (nos2) and tumor necrosis factor (tnf) upon Ln-UCNPs treatment, and with the downstream adverse outcomes, in particular, the promotion of apoptosis, liver damage, and inflammatory response. Finally, RT-qPCR analysis confirmed the up-regulated expression of nos2 and tnf in the exposed larvae, consistent with the observation of an increased number of fluorescence-labelled neutrophils and macrophages in lyz: DsRed transgenic zebrafish until 120 hpf exposure, which together demonstrated the proinflammatory effects of Ln-UCNPs on organisms. In conclusion, we illustrated the developmental toxicity, disruption of gut-microbiome, and proinflammatory effects of Ln-UCNP cores on zebrafish, and the causal network from IPA analysis may help further elucidate the adverse outcome pathway of Ln-UCNPs.
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Affiliation(s)
- Imran Ahamed Khan
- School of Environmental and Chemical Engineering, Shanghai University, Shanghai 200444, China
| | - Ting Yu
- School of Environmental and Chemical Engineering, Shanghai University, Shanghai 200444, China
| | - Yong Li
- School of Environmental and Chemical Engineering, Shanghai University, Shanghai 200444, China
| | - Chengzhang Hu
- School of Environmental and Chemical Engineering, Shanghai University, Shanghai 200444, China
| | - Xiaoyu Zhao
- School of Environmental and Chemical Engineering, Shanghai University, Shanghai 200444, China
| | - Qing Wei
- School of Environmental and Chemical Engineering, Shanghai University, Shanghai 200444, China
| | - Yufang Zhong
- School of Environmental and Chemical Engineering, Shanghai University, Shanghai 200444, China
| | - Ming Yang
- School of Environmental and Chemical Engineering, Shanghai University, Shanghai 200444, China.
| | - Jinliang Liu
- School of Environmental and Chemical Engineering, Shanghai University, Shanghai 200444, China.
| | - Zhong Chen
- Department of Cardiology, Shanghai Sixth People's Hospital Fujian, Jinjiang, Fujian 362200, China; Department of Cardiology, Shanghai Sixth People's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai 200233, China.
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Jurikova M, Dvorakova D, Bechynska K, Pulkrabova J. Bisphenols in daily clothes from conventional and recycled material: evaluation of dermal exposure to potentially toxic substances. ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH INTERNATIONAL 2024; 31:55663-55675. [PMID: 39240436 DOI: 10.1007/s11356-024-34904-4] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Grants] [Track Full Text] [Subscribe] [Scholar Register] [Received: 06/12/2024] [Accepted: 08/30/2024] [Indexed: 09/07/2024]
Abstract
Given the increasing concern about chemical exposure from textiles, our study examines the risks of dermal exposure to bisphenol A (BPA), bisphenol S (BPS), bisphenol B (BPB) and bisphenol F (BPF) from conventional and recycled textiles for adults, aiming to obtain new data, assess exposure, and evaluate the impact of washing on bisphenol levels. A total of 57 textile samples (33 from recycled and 24 from conventional material) were subjected to ultrasound-assisted extraction (UAE) followed by ultra-high performance liquid chromatography with tandem mass spectrometry analysis (UHPLC-MS/MS). The BPA and BPS concentrations varied widely (BPA: < 0.050 to 625 ng/g, BPS: 0.277-2,474 ng/g). The median BPA content in recycled textiles (13.5 ng/g) was almost twice as high as that of 7.66 ng/g in conventional textiles. BPS showed a median of 1.85 ng/g in recycled textiles and 3.42 ng/g in conventional textiles, indicating a shift from BPA to BPS in manufacturing practices. Simulated laundry experiments showed an overall reduction in bisphenols concentrations after washing. The study also assessed potential health implications via dermal exposure to dry and sweat-wet textiles compared to a tolerable daily intake (TDI) of 0.2 ng/kg bw/day for BPA set by the European Food Safety Authority (EFSA). Exposure from dry textiles remained below this threshold, while exposure from wet textiles often exceeded it, indicating an increased risk under conditions that simulate sweating or humidity. By finding the widespread presence of bisphenols in textiles, our study emphasises the importance of being aware of the potential risks associated with recycling materials as well as the benefits.
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Affiliation(s)
- Martina Jurikova
- Faculty of Food and Biochemical Technology, Department of Food Analysis and Nutrition, University of Chemistry and Technology (UCT), Prague, Technicka 5, 166 28, Prague, Czechia
| | - Darina Dvorakova
- Faculty of Food and Biochemical Technology, Department of Food Analysis and Nutrition, University of Chemistry and Technology (UCT), Prague, Technicka 5, 166 28, Prague, Czechia
| | - Kamila Bechynska
- Faculty of Food and Biochemical Technology, Department of Food Analysis and Nutrition, University of Chemistry and Technology (UCT), Prague, Technicka 5, 166 28, Prague, Czechia
| | - Jana Pulkrabova
- Faculty of Food and Biochemical Technology, Department of Food Analysis and Nutrition, University of Chemistry and Technology (UCT), Prague, Technicka 5, 166 28, Prague, Czechia.
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Pu C, Liu Y, Wang L, Ma J, Lv H, Song J, Wang B, Wang A, Zhu A, Shao P, Zhang C. Exploring the mechanism of intestinal injury induced by Bisphenol S in freshwater crayfish (Procambarus clarkii): Molecular and biochemical approaches. AQUATIC TOXICOLOGY (AMSTERDAM, NETHERLANDS) 2024; 274:107035. [PMID: 39106612 DOI: 10.1016/j.aquatox.2024.107035] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Subscribe] [Scholar Register] [Received: 03/14/2024] [Revised: 07/31/2024] [Accepted: 08/01/2024] [Indexed: 08/09/2024]
Abstract
Bisphenol S (BPS) is extensively utilized in various industries such as plastic manufacturing, food packaging, and electronics. The release of BPS into aquatic environments has been observed to have negative impacts on aquatic ecosystems. Research has shown that exposure to BPS can have adverse effects on the health of aquatic animals. This study aimed to explore the mechanism of oxidative stress and endoplasmic reticulum stress induced in freshwater crayfish (Procambarus clarkii) by exposure to BPS (0 µg/L, 1 µg/L, 10 µg/L, and 100 µg/L) for 14 days. The results showed that BPS exposure resulted in elevated levels of reactive oxygen species (ROS) and malondialdehyde (MDA) and severe intestinal histological damage. In addition, oxidative stress can occur in the body by inhibiting the activity of antioxidant enzymes and the expression of related genes. BPS exposure induced a significant increase in the relative mRNA expression levels of inflammatory cytokines (NF-κB and TNF-α) and key unfolded protein response (UPR) related genes (Bip, Ire1, and Xbp1). At the same time, BPS exposure also induced up-regulation of apoptosis genes (Cytc and Casp3), suggesting that UPR and Nrf2-Keap1 signaling pathways may play a protective role in the process of apoptosis and oxidative stress. In conclusion, Our findings present the initial evidence that exposure to environmentally relevant levels of BPS can lead to intestinal injury through various pathways, highlighting concerns about the potential harm at a population level from BPS and other bisphenol analogs.
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Affiliation(s)
- Changchang Pu
- Henan University of Science and Technology, Luoyang, Henan, PR China
| | - Yuanyi Liu
- Henan University of Science and Technology, Luoyang, Henan, PR China
| | - Lu Wang
- Henan University of Science and Technology, Luoyang, Henan, PR China
| | - Jianshuang Ma
- Henan University of Science and Technology, Luoyang, Henan, PR China
| | - Haolei Lv
- Henan University of Science and Technology, Luoyang, Henan, PR China
| | - Jianyong Song
- Henan University of Science and Technology, Luoyang, Henan, PR China
| | - Bingke Wang
- Henan Academy of Fishery Sciences, Zhengzhou, Henan, PR China
| | - Aimin Wang
- Yancheng Institute of Technology, Yancheng, Jiangsu, PR China
| | - Aimin Zhu
- Yancheng Fisheries Research Institute, Yancheng, Jiangsu, PR China
| | - Peng Shao
- Yancheng Shangshui Environmental Biotechnology Engineering Co., Ltd 224005, PR China
| | - Chunnuan Zhang
- Henan University of Science and Technology, Luoyang, Henan, PR China.
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10
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Huang S. Analysis of environmental pollutant Bisphenol F elicited prostate injury targets and underlying mechanisms through network toxicology, molecular docking, and multi-level bioinformatics data integration. Toxicology 2024; 506:153847. [PMID: 38830480 DOI: 10.1016/j.tox.2024.153847] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 04/22/2024] [Revised: 05/18/2024] [Accepted: 05/26/2024] [Indexed: 06/05/2024]
Abstract
Bisphenol F (BPF) has gained prominence as an alternative to bisphenol A (BPA) in various manufacturing applications, yet being detected in diverse environments and posed potential public health risk. This research aims to elucidate the putative toxic targets and underlying molecular mechanisms of prostate injury induced by exposure to BPF through multi-level bioinformatics data, integrating network toxicology and molecular docking. Systematically leveraging multilevel databases, we determined 276 targets related to BPF and prostate injury. Subsequent screenings through STRING and Cytoscape tool highlighted 27 key targets, including BCL2, HSP90AA1, MAPK3, ESR1, and CASP3. GO and KEGG enrichment analyses demonstrated enrichment of targets involved in apoptosis, abnormal hormonal activities, as well as cancer-related signal transduction cascades, ligand-receptor interaction networks, and endocrine system signaling pathways. Molecular docking simulations conducted via Autodock corroborated high-affinity binding interaction between BPF and key targets. The results indicate that BPF exposure can contribute to the initiation and progression of prostate cancer and prostatic hyperplastic by modulating apoptosis and proliferation, altering nerve function in blood vessel endothelial cells, and disrupting androgen metabolism. This study offers theoretical underpinnings for comprehending the molecular mechanisms implicated in BPF-elicited prostatic toxicity, while concomitantly establishing foundational framework for the development of prophylactic and therapeutic strategies for prostatic injuries related to polycarbonate and epoxy resin plastics incorporated with BPF, as well as environments afflicted by elevated levels of these compounds.
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Affiliation(s)
- Shujun Huang
- West China School of Public Health, West China Medical Center, Sichuan University, China.
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11
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Yu T, Wei Q, Tang Y, Cai L, Chen B, Yang M. A novel long non-coding RNA linc-93.2 participates in bisphenol induced oxidative stress and macrophage polarization in red common carp (Cyprinus carpio). FISH & SHELLFISH IMMUNOLOGY 2024; 151:109716. [PMID: 38909636 DOI: 10.1016/j.fsi.2024.109716] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Subscribe] [Scholar Register] [Received: 05/07/2024] [Revised: 06/19/2024] [Accepted: 06/20/2024] [Indexed: 06/25/2024]
Abstract
Previous studies show that bisphenol A (BPA) and its analogs induce oxidative stress and promote inflammatory response. However, the key molecules in regulating this process remain unclear. Here, we report significant inductive effects of BPA and bisphenol AF (BPAF) on a newly found long non-coding RNA linc-93.2 accompanied by oxidative stress and activation of pro-inflammatory pathways in treated fish and fish primary macrophages. Silencing linc-93.2 in fish primary macrophages in vitro or fish in vivo significantly promotes the expression of anti-oxidative stress-related genes and anti-inflammatory cytokines. This inhibition of pro-inflammatory cytokine expression, showing cell status disruption towards to M2 polarization. Followed by exposure to BPA or BPAF, silencing linc-93.2 in vitro or in vivo significantly attenuates the increased production of reactive oxygen species and malondialdehyde level aroused by bisphenol treatment, possibly owing to the enhancement of total antioxidant capacity observed in cells and tissue after linc-93.2 knockdown. RNA-sequencing further revealed regulation of nuclear factor-kappa b (NF-κB) in linc-93.2's downstream network, combining with our previous observation on the upstream regulation of linc-93.2 via NF-κB, which together suggest a critical role of linc-93.2 in promoting NF-κB positive feedback loop that may be an important molecular event initiating the immunotoxicity of bisphenols.
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Affiliation(s)
- Ting Yu
- School of Environmental and Chemical Engineering, Shanghai University, Shanghai, 200444, China; Third Institute of Oceanography, Ministry of Natural Resources, Xiamen, 361005, China
| | - Qing Wei
- School of Environmental and Chemical Engineering, Shanghai University, Shanghai, 200444, China
| | - Yiran Tang
- School of Environmental and Chemical Engineering, Shanghai University, Shanghai, 200444, China
| | - Ling Cai
- Third Institute of Oceanography, Ministry of Natural Resources, Xiamen, 361005, China.
| | - Bei Chen
- Fisheries Research Institute of Fujian, Key Laboratory of Cultivation and High-Value Utilization of Marine Organisms in Fujian Province, Xiamen, 361013, China
| | - Ming Yang
- School of Environmental and Chemical Engineering, Shanghai University, Shanghai, 200444, China.
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12
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Xu B, Zhang L, Wu D, Qi Z, Cao J, Li W, Fan L, Shi Y, Wu Y, Li G. CuO nanoparticles elicit intestinal immunotoxicity in zebrafish based on intestinal microbiota dysbiosis. Food Funct 2024; 15:7619-7630. [PMID: 38940701 DOI: 10.1039/d4fo01032a] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 06/29/2024]
Abstract
Copper II oxide nanoparticles (CuO NPs), a kind of widely used nanomaterial, have been detected in food and the environment, which has aroused widespread public concern. Recently, increasing data have suggested that intestinal microecology is closely related to immune homeostasis. However, the intestinal immunotoxicity induced by CuO NPs through intestinal microbiota is still unknown. Therefore, in this study, zebrafish were exposed to CuO NPs to explore intestinal immunotoxicity by evaluating physiological indicators, intestinal tissue injury, antioxidant enzyme activities, gene expression of immune factors, and changes in intestinal microbiota and its metabolites (short-chain fatty acids (SCFAs) and lipopolysaccharides (LPS)). The results revealed that the intestinal immunotoxicity of CuO NPs was mediated by the impact on intestinal microbiota and its metabolite levels. Specifically, changes were observed in the abundance of microbes that participated in the metabolism of SCFAs and LPS. The reduction in acetic acid, propionic acid and valeric acid upregulated GPR84 expression, and the decline in LPS levels further resulted in the suppression of the key immune regulatory pathways TLR4/MyD88/NF-κB, ultimately leading to intestinal immunotoxicity. This study would provide a scientific basis for the risk assessment of CuO NPs and a new perspective for research on the immunotoxicity of nanoparticles.
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Affiliation(s)
- Bufan Xu
- School of Food Science and Engineering, Shaanxi University of Science and Technology, Xi'an, 710021, China.
| | - Luyao Zhang
- School of Food Science and Engineering, Shaanxi University of Science and Technology, Xi'an, 710021, China.
| | - Di Wu
- School of Food Science and Engineering, Shaanxi University of Science and Technology, Xi'an, 710021, China.
- Institute for Global Food Security, School of Biological Sciences, Queen's University Belfast, 19 Chlorine Gardens, Belfast, BT9 5DL, UK
| | - Zihe Qi
- School of Food Science and Engineering, Shaanxi University of Science and Technology, Xi'an, 710021, China.
| | - Juanjuan Cao
- School of Food Science and Engineering, Shaanxi University of Science and Technology, Xi'an, 710021, China.
| | - Wenrui Li
- School of Food Science and Engineering, Shaanxi University of Science and Technology, Xi'an, 710021, China.
| | - Lihua Fan
- School of Food Science and Engineering, Shaanxi University of Science and Technology, Xi'an, 710021, China.
| | - Yiheng Shi
- School of Food Science and Engineering, Shaanxi University of Science and Technology, Xi'an, 710021, China.
| | - Yongning Wu
- School of Food Science and Engineering, Shaanxi University of Science and Technology, Xi'an, 710021, China.
- NHC Key Laboratory of Food Safety Risk Assessment, Food Safety Research Unit (2019RU014) of Chinese Academy of Medical Science, China National Center for Food Safety Risk Assessment, Beijing 100021, China
| | - Guoliang Li
- School of Food Science and Engineering, Shaanxi University of Science and Technology, Xi'an, 710021, China.
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13
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Zhao M, Xie Y, Xu X, Zhang Z, Shen C, Chen X, Zhu B, Yang L, Zhou B. Reproductive and transgenerational toxicity of bisphenol S exposure in pregnant rats: Insights into hormonal imbalance and steroid biosynthesis pathway disruption. THE SCIENCE OF THE TOTAL ENVIRONMENT 2024; 927:172379. [PMID: 38614345 DOI: 10.1016/j.scitotenv.2024.172379] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Subscribe] [Scholar Register] [Received: 01/23/2024] [Revised: 03/23/2024] [Accepted: 04/08/2024] [Indexed: 04/15/2024]
Abstract
Bisphenol S (BPS) is an alternative chemical to bisphenol A commonly used in food packaging materials. It raises concerns due to potential adverse effects on human health. However, limited evidence exists regarding reproductive toxicity from BPS exposure, and the mechanism of associated transgenerational toxicity remains unclear. In this study, pregnant SD rats were exposed to two different doses of BPS (0.05 or 20 mg/kg) from GD6 to PND21. The objective was to investigate reproductive and transmissible toxicity induced by BPS, explore endocrine effects, and uncover potential underlying mechanisms in rats. Perinatal exposure to BPS in the F0 generation significantly decreased the rate of body weight, ovarian organ coefficient, and growth and development of the F1 generation. Notably, these changes included abnormal increases in body weight and length, estrous cycle disruption, and embryonic dysplasia in F1. 4D-DIA proteomic and PRM analyses revealed that exposure to 20 mg/kg group significantly altered the expression of proteins, such as Lhcgr and Akr1c3, within the steroid biosynthetic pathway. This led to elevated levels of FSH and LH in the blood. The hypothalamic-pituitary-ovarian (HPO) axis, responsible for promoting fertility through the cyclic secretion of gonadotropins and steroid hormones, was affected. RT-qPCR and Western blot results demonstrated that the expression of GnRH in the hypothalamus was decreased, the GnRHR in the pituitary gland was decreased, and the expression of FSHβ and LHβ in the pituitary gland was increased. Overall, BPS exposure disrupts the HPO axis, hormone levels, and steroid biosynthesis in the ovaries, affecting offspring development and fertility. This study provides new insights into the potential effects of BPS exposure on the reproductive function of the body and its relevant mechanisms of action.
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Affiliation(s)
- Min Zhao
- School of Basic Medical Sciences, Hubei University of Chinese Medicine, Wuhan 430065, China; Hubei Shizhen Laboratory, Wuhan 430061, China
| | - Ying Xie
- School of Basic Medical Sciences, Hubei University of Chinese Medicine, Wuhan 430065, China; Hubei Shizhen Laboratory, Wuhan 430061, China
| | - Xiuqin Xu
- Wuhan Biobank Co., Ltd., Wuhan 430070, China
| | - Zequan Zhang
- School of Basic Medical Sciences, Hubei University of Chinese Medicine, Wuhan 430065, China; Hubei Shizhen Laboratory, Wuhan 430061, China
| | - Can Shen
- School of Chinese Medicine, Hubei University of Chinese Medicine, Wuhan 430065, China
| | - Xianglin Chen
- School of Basic Medical Sciences, Hubei University of Chinese Medicine, Wuhan 430065, China; Hubei Shizhen Laboratory, Wuhan 430061, China; State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China
| | - Biran Zhu
- School of Basic Medical Sciences, Hubei University of Chinese Medicine, Wuhan 430065, China; Hubei Shizhen Laboratory, Wuhan 430061, China; State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China.
| | - Lihua Yang
- State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China
| | - Bingsheng Zhou
- State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China
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14
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Zhang S, Fan Y, Qian X, Wu Z, Feng S, Xu W, Wang G. Spatiotemporal distribution, source apportionment, and ecological risk of bisphenol analogues in a highly urbanized river basin. THE SCIENCE OF THE TOTAL ENVIRONMENT 2024; 920:170964. [PMID: 38369146 DOI: 10.1016/j.scitotenv.2024.170964] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Subscribe] [Scholar Register] [Received: 11/25/2023] [Revised: 02/10/2024] [Accepted: 02/12/2024] [Indexed: 02/20/2024]
Abstract
Bisphenol analogues (BPs), as one of the endocrine disruptors, have received wide attention due to their adverse impacts on ecosystems. However, the seasonal spatiotemporal distribution, source apportionment, and ecological risk of BPs in natural basins are poorly understood. Especially in highly urbanized river basins with the extensive economic development and anthropogenic activities threaten these critical but ecologically fragile regions. In this study, field investigations of BPs in the waters of the entire Qinhuai River Basin (QRB) were conducted in June (before the annual flood period) and August (after the annual flood period) 2023. The Qinhuai River, an important primary tributary of the lower Yangtze River, is located in eastern China and the QRB is characterized by a high population density and dense urbanization. Thirty-two sites were sampled for six types of BPs known to be ubiquitous in the surface water of the QRB. Significant differences in the concentrations of those BPs were found. Specifically, the concentration of total BPs (ΣBPs) was significantly higher before than after the flood period: 20.3-472 ng/L (mean = 146 ng/L) and 14.1-105 ng/L (mean = 35.9 ng/L), respectively. BPA was the main contributor to ΣBPs before the flood, and BPB followed by BPA after the flood. ΣBP concentrations were 12-241 % higher downstream than upstream of wastewater treatment plants (WWTPs). The results of a principal component analysis followed by multiple linear regression (PCA-MLR) suggested that untreated wastewater discharge from the WWTPs is an important source of BPs in the basin, with urban rainfall runoff as another potential source after the flood period. An assessment of the ecological risk of BPs, based on a calculation of the risk quotient, showed that BPA and BPS should be given due attention, and overall ecological risk of BPs pose a low risk to local algae but high and medium risks to invertebrates and fish, respectively.
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Affiliation(s)
- Sheng Zhang
- State Key Laboratory of Pollution Control and Resources Reuse, School of Environment, Nanjing University, Nanjing 210023, China
| | - Yifan Fan
- State Key Laboratory of Pollution Control and Resources Reuse, School of Environment, Nanjing University, Nanjing 210023, China.
| | - Xin Qian
- State Key Laboratory of Pollution Control and Resources Reuse, School of Environment, Nanjing University, Nanjing 210023, China; Jiangsu Collaborative Innovation Center of Atmospheric Environment and Equipment Technology (CICAEET), Nanjing University of Information Science & Technology, Nanjing 210044, China.
| | - Zeqiang Wu
- State Key Laboratory of Pollution Control and Resources Reuse, School of Environment, Nanjing University, Nanjing 210023, China
| | - Shaoyan Feng
- State Key Laboratory of Pollution Control and Resources Reuse, School of Environment, Nanjing University, Nanjing 210023, China
| | - Wanlu Xu
- State Key Laboratory of Pollution Control and Resources Reuse, School of Environment, Nanjing University, Nanjing 210023, China
| | - Guoqiang Wang
- Innovation Research Center of Satellite Application, Faculty of Geographical Science, Beijing Normal University, Beijing 100875, China
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15
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D'Souza LC, Paithankar JG, Stopper H, Pandey A, Sharma A. Environmental Chemical-Induced Reactive Oxygen Species Generation and Immunotoxicity: A Comprehensive Review. Antioxid Redox Signal 2024; 40:691-714. [PMID: 37917110 DOI: 10.1089/ars.2022.0117] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Journal Information] [Submit a Manuscript] [Subscribe] [Scholar Register] [Indexed: 11/03/2023]
Abstract
Significance: Reactive oxygen species (ROS), the reactive oxygen-carrying chemicals moieties, act as pleiotropic signal transducers to maintain various biological processes/functions, including immune response. Increased ROS production leads to oxidative stress, which is implicated in xenobiotic-induced adverse effects. Understanding the immunoregulatory mechanisms and immunotoxicity is of interest to developing therapeutics against xenobiotic insults. Recent Advances: While developmental studies have established the essential roles of ROS in the establishment and proper functioning of the immune system, toxicological studies have demonstrated high ROS generation as one of the potential mechanisms of immunotoxicity induced by environmental chemicals, including heavy metals, pesticides, aromatic hydrocarbons (benzene and derivatives), plastics, and nanoparticles. Mitochondrial electron transport and various signaling components, including NADH oxidase, toll-like receptors (TLRs), NF-κB, JNK, NRF2, p53, and STAT3, are involved in xenobiotic-induced ROS generation and immunotoxicity. Critical Issues: With many studies demonstrating the role of ROS and oxidative stress in xenobiotic-induced immunotoxicity, rigorous and orthogonal approaches are needed to achieve in-depth and precise understanding. The association of xenobiotic-induced immunotoxicity with disease susceptibility and progression needs more data acquisition. Furthermore, the general methodology needs to be possibly replaced with high-throughput precise techniques. Future Directions: The progression of xenobiotic-induced immunotoxicity into disease manifestation is not well documented. Immunotoxicological studies about the combination of xenobiotics, age-related sensitivity, and their involvement in human disease incidence and pathogenesis are warranted. Antioxid. Redox Signal. 40, 691-714.
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Affiliation(s)
- Leonard Clinton D'Souza
- Nitte (Deemed to be University), Nitte University Centre for Science Education and Research (NUCSER), Department of Environmental Health and Toxicology, Mangalore, India
| | - Jagdish Gopal Paithankar
- Nitte (Deemed to be University), Nitte University Centre for Science Education and Research (NUCSER), Department of Environmental Health and Toxicology, Mangalore, India
| | - Helga Stopper
- Institute of Pharmacology and Toxicology, University of Wuerzburg, Wuerzburg, Germany
| | - Ashutosh Pandey
- Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, Texas, USA
| | - Anurag Sharma
- Nitte (Deemed to be University), Nitte University Centre for Science Education and Research (NUCSER), Department of Environmental Health and Toxicology, Mangalore, India
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16
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Li Z, Li M, Li D, Chen Y, Feng W, Zhao T, Yang L, Mao G, Wu X. A review of cumulative toxic effects of environmental endocrine disruptors on the zebrafish immune system: Characterization methods, toxic effects and mechanisms. ENVIRONMENTAL RESEARCH 2024; 246:118010. [PMID: 38157964 DOI: 10.1016/j.envres.2023.118010] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Subscribe] [Scholar Register] [Received: 10/18/2023] [Revised: 12/19/2023] [Accepted: 12/20/2023] [Indexed: 01/03/2024]
Abstract
Environmental endocrine disrupting chemicals (EDCs), are a type of exogenous organic pollutants, are ubiquitous in natural aquatic environments. Currently, in addition to neurological, endocrine, developmental and reproductive toxicity, ecotoxicology studies on immunotoxicity are receiving increasing attention. In this review, the composition of immune system of zebrafish, the common indicators of immunotoxicity, the immunotoxicity of EDCs and their molecular mechanism were summarized. We reviewed the immunotoxicity of EDCs on zebrafish mainly in terms of immune organs, immunocytes, immune molecules and immune functions, meanwhile, the possible molecular mechanisms driving these effects were elucidated in terms of endocrine disruption, dysregulation of signaling pathways, and oxidative damage. Hopefully, this review will provide a reference for further investigation of the immunotoxicity of EDCs.
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Affiliation(s)
- Zixu Li
- School of the Environment and Safety Engineering, School of Emergency Management, Jiangsu University, 301 Xuefu Rd, Zhenjiang, 212013, China
| | - Muge Li
- School of the Environment and Safety Engineering, School of Emergency Management, Jiangsu University, 301 Xuefu Rd, Zhenjiang, 212013, China
| | - Dan Li
- School of the Environment and Safety Engineering, School of Emergency Management, Jiangsu University, 301 Xuefu Rd, Zhenjiang, 212013, China
| | - Yao Chen
- School of the Environment and Safety Engineering, School of Emergency Management, Jiangsu University, 301 Xuefu Rd, Zhenjiang, 212013, China; Institute of Environmental Health and Ecological Safety, Jiangsu University, 301 Xuefu Rd, Zhenjiang, 212013, China
| | - Weiwei Feng
- School of the Environment and Safety Engineering, School of Emergency Management, Jiangsu University, 301 Xuefu Rd, Zhenjiang, 212013, China; Institute of Environmental Health and Ecological Safety, Jiangsu University, 301 Xuefu Rd, Zhenjiang, 212013, China
| | - Ting Zhao
- School of Chemistry and Chemical Engineering, Jiangsu University, 301 Xuefu Rd, Zhenjiang, 212013, China
| | - Liuqing Yang
- School of Chemistry and Chemical Engineering, Jiangsu University, 301 Xuefu Rd, Zhenjiang, 212013, China
| | - Guanghua Mao
- School of the Environment and Safety Engineering, School of Emergency Management, Jiangsu University, 301 Xuefu Rd, Zhenjiang, 212013, China.
| | - Xiangyang Wu
- School of the Environment and Safety Engineering, School of Emergency Management, Jiangsu University, 301 Xuefu Rd, Zhenjiang, 212013, China.
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17
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Li B, Huang Y, Pi D, Li X, Guo Y, Liang Z, Song X, Wang J, Wang X. Effects of Acute and Developmental Exposure to Bisphenol S on Chinese Medaka ( Oryzias sinensis). J Xenobiot 2024; 14:452-466. [PMID: 38525695 PMCID: PMC10961820 DOI: 10.3390/jox14020027] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Grants] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 12/05/2023] [Revised: 03/15/2024] [Accepted: 03/19/2024] [Indexed: 03/26/2024] Open
Abstract
Bisphenol S (BPS), one of the substitutes for bisphenol A (BPA), is widely used in various commodities. The BPS concentrations in surface water have gradually increased in recent years, making it a predominant bisphenol analogue in the aquatic environment and raising concerns about its health and ecological effects on aquatic organisms. For this study, we conducted a 96 h acute toxicity test and a 15-day developmental exposure test to assess the adverse effects of BPS exposure in Chinese medaka (Oryzias sinensis), a new local aquatic animal model. The results indicate that the acute exposure of Chinese medaka embryos to BPS led to relatively low toxicity. However, developmental exposure to BPS was found to cause developmental abnormalities, such as decreased hatching rate and body length, at 15 dpf. A transcriptome analysis showed that exposure to different concentrations of bisphenol S often induced different reactions. In summary, environmental concentrations of BPS can have adverse effects on the hatching and physical development of Chinese medaka, and further attention needs to be paid to the potential toxicity of environmental BPS.
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Affiliation(s)
- Bingying Li
- Institute of Modern Aquaculture Science and Engineering, Guangdong-Macao Joint Laboratory for Aquaculture Breeding Development and Innovation, College of Life Sciences, South China Normal University, Guangzhou 510631, China; (B.L.); (Y.H.); (D.P.); (Y.G.); (Z.L.)
| | - Yongsi Huang
- Institute of Modern Aquaculture Science and Engineering, Guangdong-Macao Joint Laboratory for Aquaculture Breeding Development and Innovation, College of Life Sciences, South China Normal University, Guangzhou 510631, China; (B.L.); (Y.H.); (D.P.); (Y.G.); (Z.L.)
| | - Duan Pi
- Institute of Modern Aquaculture Science and Engineering, Guangdong-Macao Joint Laboratory for Aquaculture Breeding Development and Innovation, College of Life Sciences, South China Normal University, Guangzhou 510631, China; (B.L.); (Y.H.); (D.P.); (Y.G.); (Z.L.)
| | - Xiang Li
- Institute of Modern Aquaculture Science and Engineering, Guangdong-Macao Joint Laboratory for Aquaculture Breeding Development and Innovation, College of Life Sciences, South China Normal University, Guangzhou 510631, China; (B.L.); (Y.H.); (D.P.); (Y.G.); (Z.L.)
| | - Yafen Guo
- Institute of Modern Aquaculture Science and Engineering, Guangdong-Macao Joint Laboratory for Aquaculture Breeding Development and Innovation, College of Life Sciences, South China Normal University, Guangzhou 510631, China; (B.L.); (Y.H.); (D.P.); (Y.G.); (Z.L.)
| | - Zhiying Liang
- Institute of Modern Aquaculture Science and Engineering, Guangdong-Macao Joint Laboratory for Aquaculture Breeding Development and Innovation, College of Life Sciences, South China Normal University, Guangzhou 510631, China; (B.L.); (Y.H.); (D.P.); (Y.G.); (Z.L.)
| | - Xiaohong Song
- Guangxi Key Laboratory of Environmental Pollution Control Theory and Technology, Guilin University of Technology, Guilin 541000, China;
| | - Junjie Wang
- Institute of Modern Aquaculture Science and Engineering, Guangdong-Macao Joint Laboratory for Aquaculture Breeding Development and Innovation, College of Life Sciences, South China Normal University, Guangzhou 510631, China; (B.L.); (Y.H.); (D.P.); (Y.G.); (Z.L.)
| | - Xuegeng Wang
- Institute of Modern Aquaculture Science and Engineering, Guangdong-Macao Joint Laboratory for Aquaculture Breeding Development and Innovation, College of Life Sciences, South China Normal University, Guangzhou 510631, China; (B.L.); (Y.H.); (D.P.); (Y.G.); (Z.L.)
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18
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Pu C, Liu Y, Ma J, Li J, Sun R, Zhou Y, Wang B, Wang A, Zhang C. The effects of bisphenol S exposure on the growth, physiological and biochemical indices, and ecdysteroid receptor gene expression in red swamp crayfish, Procambarus clarkii. Comp Biochem Physiol C Toxicol Pharmacol 2024; 276:109811. [PMID: 38061619 DOI: 10.1016/j.cbpc.2023.109811] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Journal Information] [Submit a Manuscript] [Subscribe] [Scholar Register] [Received: 08/24/2023] [Revised: 11/11/2023] [Accepted: 12/03/2023] [Indexed: 01/03/2024]
Abstract
The experiment was conducted to investigate the effects of Bisphenol S (BPS) on growth, physiological and biochemical indices, and the expression of ecdysteroid receptor (ECR) of the red swamp crayfish (Procambarus clarkii). The gene encoding ECR was isolated from red swamp crayfish by homologous cloning and rapid amplification of cDNA ends (RACE). The ECR transcripts were 1757 bp long and encoded proteins of 576 amino acids. The quantitative real-time PCR (qRT-PCR) analysis showed that the ECR gene was expressed in various tissues under normal conditions, and the highest level was observed in the ovary and the lowest level was observed in the muscle (P < 0.05). Then, the experiment was designed with four different BPS concentrations (0, 1, 10, and 100 μg/L), BPS exposure for 14 days, three parallel groups, and a total of 240 red swamp crayfish. At 100 μg/L BPS, the survival rate, weight gain rate, and relative length rate were decreased significantly (P < 0.05). Malonaldehyde (MDA) content reached the highest level at 100 μg/L BPS. When BPS concentration was higher than 10 μg/L, the activities of superoxide dismutase (SOD) and catalase (CAT) were significantly lower than those of the control group (P < 0.05). The expression levels of the ECR gene in ovary, intestinal, gill, and hepatopancreas tissues were significantly increased after BPS exposure (P < 0.05). The ECR gene expression in ovaries and Y-organs was significantly higher than other groups in 10 μg/L BPS (P < 0.05). The expressions of the tumor necrosis factor -α (TNF-α) and interleukin-6 (IL-6) genes in the hepatopancreas gradually increased, and the highest expression was observed exposed in 100 μg/L BPS (P < 0.05). This research will provide novel insights into the health risk assessment of BPS in aquatic organisms.
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Affiliation(s)
- Changchang Pu
- Laboratory of Aquatic Environment and Animal Safety, College of Animal Science and Technology, Henan University of Science and Technology, Luoyang, Henan, China
| | - Yuanyi Liu
- Laboratory of Aquatic Environment and Animal Safety, College of Animal Science and Technology, Henan University of Science and Technology, Luoyang, Henan, China
| | - Jianshuang Ma
- Laboratory of Aquatic Environment and Animal Safety, College of Animal Science and Technology, Henan University of Science and Technology, Luoyang, Henan, China
| | - Jiajin Li
- Laboratory of Aquatic Environment and Animal Safety, College of Animal Science and Technology, Henan University of Science and Technology, Luoyang, Henan, China
| | - Ruyi Sun
- Laboratory of Aquatic Environment and Animal Safety, College of Animal Science and Technology, Henan University of Science and Technology, Luoyang, Henan, China
| | - Yang Zhou
- Laboratory of Aquatic Environment and Animal Safety, College of Animal Science and Technology, Henan University of Science and Technology, Luoyang, Henan, China
| | - Bingke Wang
- Henan Academy of Fishery Sciences, Zhengzhou 450044, China
| | - Aimin Wang
- Institute of Aquatic Animal Nutrition and Feed, College of Marine and Bioengineering, Yancheng Institute of Technology, Yancheng, Jiangsu, China.
| | - Chunnuan Zhang
- Laboratory of Aquatic Environment and Animal Safety, College of Animal Science and Technology, Henan University of Science and Technology, Luoyang, Henan, China.
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Maddela NR, Kakarla D, Venkateswarlu K, Megharaj M. Additives of plastics: Entry into the environment and potential risks to human and ecological health. JOURNAL OF ENVIRONMENTAL MANAGEMENT 2023; 348:119364. [PMID: 37866190 DOI: 10.1016/j.jenvman.2023.119364] [Citation(s) in RCA: 22] [Impact Index Per Article: 11.0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Subscribe] [Scholar Register] [Received: 06/17/2023] [Revised: 10/03/2023] [Accepted: 10/14/2023] [Indexed: 10/24/2023]
Abstract
A steep rise in global plastic production and significant discharge of plastic waste are expected in the near future. Plastics pose a threat to the ecosystem and human health through the generation of particulate plastics that act as carriers for other emerging contaminants, and the release of toxic chemical additives. Since plastic additives are not covalently bound, they can freely leach into the environment. Due to their occurrence in various environmental settings, the additives exert significant ecotoxicity. However, only 25% of plastic additives have been characterized for their potential ecological concern. Despite global market statistics highlighting the substantial environmental burden caused by the unrestricted production and use of plastic additives, information on their ecotoxicity remains incomplete. By focusing on the ecological impacts of plastic additives, the present review aims to provide detailed insights into the following aspects: (i) diversity and occurrence in the environment, (ii) leaching from plastic materials, (iii) trophic transfer, (iv) human exposure, (v) risks to ecosystem and human health, and (vi) legal guidelines and mitigation strategies. These insights are of immense value in restricting the use of toxic additives, searching for eco-friendly alternatives, and establishing or revising guidelines on plastic additives by global health and environmental agencies.
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Affiliation(s)
- Naga Raju Maddela
- Departamento de Ciencias Biológicas, Facultad de Ciencias de la Salud, Universidad Técnica de Manabí, Portoviejo, 130105, Ecuador
| | - Dhatri Kakarla
- University of North Carolina at Chapel Hill, Chapel Hill, NC, 27599, USA
| | - Kadiyala Venkateswarlu
- Formerly Department of Microbiology, Sri Krishnadevaraya University, Anantapuramu, 515003, India
| | - Mallavarapu Megharaj
- Global Centre for Environmental Remediation (GCER), College of Engineering Science and Environment, ATC Building, The University of Newcastle, Callaghan, NSW, 2308, Australia; Cooperative Research Centre for Contamination Assessment and Remediation of Environment (CRC CARE), The University of Newcastle, ATC Building, Callaghan, NSW, 2308, Australia.
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20
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Luo H, Yang Y, Zhang H, Ren L, Han X, Lin Y, Wu M, Hou Y. Toxic effects of bisphenol S on mice heart and human umbilical cord endothelial cells. ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY 2023; 267:115652. [PMID: 37924801 DOI: 10.1016/j.ecoenv.2023.115652] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Subscribe] [Scholar Register] [Received: 05/09/2023] [Revised: 10/25/2023] [Accepted: 10/29/2023] [Indexed: 11/06/2023]
Abstract
Bisphenol S (BPS) exerts toxic effects on hippocampal HT22 cells, endocrine secretion, and reproductive capacity. However, whether BPS exerts toxic effects on the heart requires further investigation. Therefore, we investigated the effects of BPS on mouse heart tissues and predicted possible underlying molecular mechanisms of action. Our study showed that BPS induced apoptosis, increased oxidative stress response. Using electron microscopy, we found that BPS disrupted sarcomere arrangement in myocardial cells and caused reduction in the number of plasmalemmal vesicles in endothelial cells in the mouse heart tissues. Also, BPS increased expression levels of P-NF-κB in mouse heart tissues. Furthermore, we found that BPS induced reactive oxygen species (ROS) generation, NF-κB activation, promoted apoptosis, elevated expression of BAX and Caspase 3, and decreased expression of Bcl-2 in H9c2 cells and HUVECs. However, after the addition of n-acetylcysteine or pyrrolidinedithiocarbamate, ROS generation, NF-κB activation, apoptosis, and expression of BAX and Caspase 3 were reduced, whereas expression of Bcl-2 was elevated. Our results demonstrated that BPS induced apoptosis of myocardial and endothelial cells through oxidative stress by activation of NF-κB signaling pathway.
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Affiliation(s)
- Hanlin Luo
- Deparment of Histology and Embryology, College of Basic Medicine, Binzhou Medical University, Yantai 264003, PR China
| | - Yang Yang
- Deparment of Histology and Embryology, College of Basic Medicine, Binzhou Medical University, Yantai 264003, PR China
| | - Hongyu Zhang
- Deparment of Histology and Embryology, College of Basic Medicine, Binzhou Medical University, Yantai 264003, PR China
| | - Luyu Ren
- Deparment of Histology and Embryology, College of Basic Medicine, Binzhou Medical University, Yantai 264003, PR China
| | - Xueben Han
- Deparment of Histology and Embryology, College of Basic Medicine, Binzhou Medical University, Yantai 264003, PR China
| | - Yu Lin
- Deparment of Histology and Embryology, College of Basic Medicine, Binzhou Medical University, Yantai 264003, PR China
| | - Menghan Wu
- Deparment of Histology and Embryology, College of Basic Medicine, Binzhou Medical University, Yantai 264003, PR China
| | - Yun Hou
- Deparment of Histology and Embryology, College of Basic Medicine, Binzhou Medical University, Yantai 264003, PR China.
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21
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Lu W, Yang F, Meng Y, An J, Hu B, Jian S, Yang G, Lu H, Wen C. Immunotoxicity and transcriptome analysis of zebrafish embryos exposure to Nitazoxanide. FISH & SHELLFISH IMMUNOLOGY 2023; 141:108977. [PMID: 37579811 DOI: 10.1016/j.fsi.2023.108977] [Citation(s) in RCA: 1] [Impact Index Per Article: 0.5] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Subscribe] [Scholar Register] [Received: 06/06/2023] [Revised: 07/23/2023] [Accepted: 07/27/2023] [Indexed: 08/16/2023]
Abstract
Nitazoxanide (NTZ) is a broad-spectrum immunomodulatory drug, and little information is about the immunotoxicity of aquatic organisms induced by NTZ. In the present study, reduced body length and decreased yolk sac absorption in the NTZ-treated group were observed. Meanwhile, the number of innate immune cells and adaptive immune cells was substantially reduced upon NTZ exposure, and the migration and retention of macrophages and neutrophils in the injured area were inhibited. Following NTZ stimulation, oxidative stress levels in the zebrafish increased obviously. Mechanistically, RNA-seq, a high-throughput method, was performed to analyze the global expression of differentially expressed genes (DEGs) in zebrafish embryos treated with NTZ. 531 DEGs were identified by comparative transcriptome analysis, including 121 up-regulated and 420 down-regulated genes in zebrafish embryos after NTZ exposure. The transcriptome sequences were further subjected to the Kyoto Encyclopedia of Genes and Genomes (KEGG) and gene ontology (GO) and analysis, showing phototransduction and metabolic pathway, respectively, and were most enriched. In addition, some immune-related genes were inhibited after NTZ exposure. RNA-seq results confirmed by qRT-PCR were used to verify the expression of the 6 selected genes. The other immune-related genes such as two pro-inflammatory cytokines (IL-1β, tnfα) and two chemokines (CXCL8b.3, CXCL-c1c) were further confirmed and were differentially regulated after NTZ exposure. In summary, NTZ exposure could lead to immunotoxicity and increased ROS in zebrafish embryos, this study provides valuable information for future elucidating the molecular mechanism of exogenous stimuli-induced immunotoxicity in aquatic ecosystems.
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Affiliation(s)
- Wuting Lu
- Department of Aquatic Science, College of Life Science, Nanchang University, Xuefu Avenue, Nanchang, Jiangxi Province, 330031, China
| | - Fanhua Yang
- College of Food Science and Technology, Nanchang University, Nanchang, 330031, China
| | - Yunlong Meng
- Department of Medical Genetics, School of Medicine, Tongji University, Shanghai, 200092, China
| | - Jinhua An
- Department of Aquatic Science, College of Life Science, Nanchang University, Xuefu Avenue, Nanchang, Jiangxi Province, 330031, China
| | - Baoqing Hu
- Department of Aquatic Science, College of Life Science, Nanchang University, Xuefu Avenue, Nanchang, Jiangxi Province, 330031, China
| | - Shaoqing Jian
- Department of Aquatic Science, College of Life Science, Nanchang University, Xuefu Avenue, Nanchang, Jiangxi Province, 330031, China
| | - Gang Yang
- Department of Aquatic Science, College of Life Science, Nanchang University, Xuefu Avenue, Nanchang, Jiangxi Province, 330031, China
| | - Huiqiang Lu
- Ganzhou Key Laboratory for Drug Screening and Discovery, Gannan Normal University, Ganzhou, 341000, Jiangxi, China; Jiangxi Engineering Laboratory of Zebrafish Modeling and Drug Screening for Human Diseases, Jiangxi Key Laboratory of Developmental Biology of Organs, Affiliated Hospital of Jinggangshan University, Ji'an, 343009, China.
| | - Chungen Wen
- Department of Aquatic Science, College of Life Science, Nanchang University, Xuefu Avenue, Nanchang, Jiangxi Province, 330031, China.
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22
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Tan Y, Taibl KR, Dunlop AL, Barr DB, Panuwet P, Yakimavets V, Kannan K, Corwin EJ, Ryan PB, Eatman JA, Liang D, Eick SM. Association between a Mixture of Per- and Polyfluoroalkyl Substances (PFAS) and Inflammatory Biomarkers in the Atlanta African American Maternal-Child Cohort. ENVIRONMENTAL SCIENCE & TECHNOLOGY 2023; 57:13419-13428. [PMID: 37649345 PMCID: PMC10900195 DOI: 10.1021/acs.est.3c04688] [Citation(s) in RCA: 8] [Impact Index Per Article: 4.0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Grants] [Track Full Text] [Subscribe] [Scholar Register] [Indexed: 09/01/2023]
Abstract
Per- and polyfluoroalkyl substances (PFAS) have been identified as environmental contributors to adverse birth outcomes. One potential mechanistic pathway could be through PFAS-related inflammation and cytokine production. Here, we examined associations between a PFAS mixture and inflammatory biomarkers during early and late pregnancy from participants enrolled in the Atlanta African American Maternal-Child Cohort (N = 425). Serum concentrations of multiple PFAS were detected in >90% samples at 8-14 weeks gestation. Serum concentrations of interferon-γ (IFN-γ), interleukin 6 (IL-6), interleukin 10 (IL-10), tumor necrosis factor-α (TNF-α), and C-reactive protein (CRP) were measured at up to two time points (8-14 weeks and 24-30 weeks gestation). The effect of the PFAS mixture on each inflammatory biomarker was examined using quantile g-computation, Bayesian kernel machine regression (BKMR), Bayesian Weighted Sums (BWS), and weighted quantile sum (WQS) regression. Across all models, the PFAS mixture was associated with increased IFN-γ, IL-10, and TNF-α at both time points, with the strongest effects being observed at 24-30 weeks. Using quantile g-computation, increasing concentrations of a PFAS mixture were associated with a 29% (95% confidence interval = 18.0%, 40.7%) increase in TNF-α at 24-30 weeks. Similarly, using BWS, the PFAS mixture was associated with increased TNF-α at 24-30 weeks (summed effect = 0.29, 95% highest posterior density = 0.17, 0.41). The PFAS mixture was also positively associated with TNF-α at 24-30 weeks using BKMR [75th vs 50th percentile: 17.1% (95% credible interval = 7.7%, 27.4%)]. Meanwhile, PFOS was consistently the main drivers of overall mixture effect across four methods. Our findings indicated an increase in prenatal PFAS exposure is associated with an increase in multiple pro-inflammatory cytokines, potentially contributing to adverse pregnancy outcomes.
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Affiliation(s)
- Youran Tan
- Gangarosa Department of Environmental Health, Rollins School of Public Health, Emory University, Atlanta, GA, 30329, USA
| | - Kaitlin R. Taibl
- Gangarosa Department of Environmental Health, Rollins School of Public Health, Emory University, Atlanta, GA, 30329, USA
| | - Anne L. Dunlop
- Department of Gynecology and Obstetrics, School of Medicine, Emory University, Atlanta, GA, 30329, USA
| | - Dana Boyd Barr
- Gangarosa Department of Environmental Health, Rollins School of Public Health, Emory University, Atlanta, GA, 30329, USA
| | - Parinya Panuwet
- Gangarosa Department of Environmental Health, Rollins School of Public Health, Emory University, Atlanta, GA, 30329, USA
| | - Volha Yakimavets
- Gangarosa Department of Environmental Health, Rollins School of Public Health, Emory University, Atlanta, GA, 30329, USA
| | - Kurunthachalam Kannan
- Department of Pediatrics, New York University School of Medicine, New York, NY, 10016, USA
- Department of Environmental Medicine, New York University School of Medicine, New York, 10016, NY, USA
| | | | - P. Barry Ryan
- Gangarosa Department of Environmental Health, Rollins School of Public Health, Emory University, Atlanta, GA, 30329, USA
| | - Jasmin A. Eatman
- Gangarosa Department of Environmental Health, Rollins School of Public Health, Emory University, Atlanta, GA, 30329, USA
| | - Donghai Liang
- Gangarosa Department of Environmental Health, Rollins School of Public Health, Emory University, Atlanta, GA, 30329, USA
| | - Stephanie M. Eick
- Gangarosa Department of Environmental Health, Rollins School of Public Health, Emory University, Atlanta, GA, 30329, USA
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23
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Makowska K, Fagundes KRC, Gonkowski S. Influence of bisphenol A and its analog bisphenol S on cocaine- and amphetamine-regulated transcript peptide-positive enteric neurons in the mouse gastrointestinal tract. Front Mol Neurosci 2023; 16:1234841. [PMID: 37675141 PMCID: PMC10477371 DOI: 10.3389/fnmol.2023.1234841] [Citation(s) in RCA: 2] [Impact Index Per Article: 1.0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 06/05/2023] [Accepted: 08/08/2023] [Indexed: 09/08/2023] Open
Abstract
Introduction Bisphenol A (BPA) is used in large quantities for the production of plastics and is present in various everyday objects. It penetrates living organisms and shows multidirectional adverse influence on many internal organs. For this reason, BPA is often replaced in plastic production by other substances. One of them is bisphenol S (BPS), whose effects on the enteric nervous system (ENS) have not been explained. Methods Therefore, the present study compares the influence of BPA and BPS on the number of enteric neurons immunoreactive to cocaine-and amphetamine-regulated transcript (CART) peptide located in the ENS of the stomach, jejunum and colon with the use of double immunofluorescence method. Results The obtained results have shown that both bisphenols studied induced an increase in the number of CART-positive enteric neurons, and the severity of changes depended on the type of enteric ganglion, the dose of bisphenols and the segment of the digestive tract. The most visible changes were noted in the myenteric ganglia in the colon. Moreover, in the colon, the changes submitted by BPS are more noticeable than those observed after BPA administration. In the stomach and jejunum, bisphenol-induced changes were less visible, and changes caused by BPS were similar or less pronounced than those noted under the impact of BPA, depending on the segment of the gastrointestinal tract and ganglion type studied. Discussion The results show that BPS affects the enteric neurons containing CART in a similar way to BPA, and the BPS impact is even stronger in the colon. Therefore, BPS is not neutral for the gastrointestinal tract and ENS.
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Affiliation(s)
- Krystyna Makowska
- Department of Clinical Diagnostics, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, Olsztyn, Poland
| | - Kainã R. C. Fagundes
- Laboratório de Morfofisiologia Animal, Instituto de Biociências, Universidade Estadual Paulista, São Paulo, Brazil
| | - Sławomir Gonkowski
- Department of Clinical Physiology, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, Olsztyn, Poland
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24
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Odetayo AF, Adeyemi WJ, Olayaki LA. In vivo exposure to bisphenol F induces oxidative testicular toxicity: role of Erβ and p53/Bcl-2 signaling pathway. FRONTIERS IN REPRODUCTIVE HEALTH 2023; 5:1204728. [PMID: 37601897 PMCID: PMC10433915 DOI: 10.3389/frph.2023.1204728] [Citation(s) in RCA: 16] [Impact Index Per Article: 8.0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 04/12/2023] [Accepted: 07/07/2023] [Indexed: 08/22/2023] Open
Abstract
Introduction Bisphenol F (BPF), an alternative to bisphenol A has been implicated as a gonadotoxic substance. BPF has been shown to induce hormonal imbalance and testicular oxidative damage. However, the mechanism associated with BPF-induced testicular toxicity has not been fully explored. This study was designed to explore the role of tumor protein (p53)/ B-cell lymphoma 2 (BCl-2) signaling and oestrogen receptor beta (Erβ) in BPF-induced testicular toxicity. Methods Male Wistar rats were randomized into control (Cntrl), BPF-treated (10, 30, and 50 mg/kg for low dose (BPF-L), medium dose (BPF-M), and high dose (BPF-H) respectively), and BPF-treated recovery (Cntrl-R, BPF-L-R, BPF-M-R, and BPF-H-R). The administration was via gavage and lasted for 28 days and the animals in the recovery groups were allowed 28-days exposure free period for recovery from BPF exposure. Results BPF resulted in the distortion of the testicular histoarchitecture, which was accompanied by a significant rise in testicular gamma-lutamyl transferase and lactate dehydrogenase activities but a decline in sorbitol dehydrogenase activities. Also, BPF caused a significant reduction in plasma gonadotropin-releasing hormone, luteinising hormone, follicle-stimulating hormone, and testosterone, which was associated with the downregulation of testicular 3beta-hydroxysteroid dehydrogenase and 17beta-hydroxysteroid dehydrogenase activities. Furthermore, BPF induced testicular inflammation, redox imbalance, and apoptosis, accompanied by distortion in p53/BCl-2 signaling and overexpression of Erβ. Again, the observed toxic effects of BPF were dose-dependent and not completely reversed by BPF cessation. Discussion Bisphenol F induced gonadotoxicity by distorting p53/BCl2 signaling and the expression of Erβ. These observed alterations were not completely reversed after the cessation of BPF exposure.
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Affiliation(s)
- Adeyemi Fatai Odetayo
- Physiology Department, University of Ilorin, Ilorin, Nigeria
- Physiology Department, Federal University of Health Sciences, Ila Orangun, Nigeria
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25
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Kodila A, Franko N, Sollner Dolenc M. A review on immunomodulatory effects of BPA analogues. Arch Toxicol 2023; 97:1831-1846. [PMID: 37204436 PMCID: PMC10256647 DOI: 10.1007/s00204-023-03519-y] [Citation(s) in RCA: 26] [Impact Index Per Article: 13.0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Grants] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 02/06/2023] [Accepted: 05/11/2023] [Indexed: 05/20/2023]
Abstract
Bisphenol A (BPA) is a known endocrine disruptor found in many consumer products that humans come into contact with on a daily basis. Due to increasing concerns about the safety of BPA and the introduction of new legislation restricting its use, industry has responded by adopting new, less studied BPA analogues that have similar polymer-forming properties. Some BPA analogues have already been shown to exhibit effects similar to BPA, for example, contributing to endocrine disruption through agonistic or antagonistic behaviour at various nuclear receptors such as estrogen (ER), androgen (AR), glucocorticoid (GR), aryl hydrocarbon (AhR), and pregnane X receptor (PXR). Since the European Food Safety Authority (EFSA) issued a draft re-evaluation of BPA and drastically reduced the temporary tolerable daily intake (t-TDI) of BPA from 4 mg/kg body weight/day to 0.2 ng/kg body weight/day due to increasing concern about the toxic properties of BPA, including its potential to disrupt immune system processes, we conducted a comprehensive review of the immunomodulatory activity of environmentally abundant BPA analogues. The results of the review suggest that BPA analogues may affect both the innate and acquired immune systems and can contribute to various immune-mediated conditions such as hypersensitivity reactions, allergies, and disruption of the human microbiome.
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Affiliation(s)
- Anja Kodila
- Faculty of Pharmacy, University of Ljubljana, Aškerčeva cesta 7, 1000, Ljubljana, Slovenia
| | - Nina Franko
- Faculty of Pharmacy, University of Ljubljana, Aškerčeva cesta 7, 1000, Ljubljana, Slovenia
| | - Marija Sollner Dolenc
- Faculty of Pharmacy, University of Ljubljana, Aškerčeva cesta 7, 1000, Ljubljana, Slovenia.
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Liang Z, Guo Y, Pi D, Li X, Li B, Huang Y, Song X, Bhandari RK, Wang X. Transcriptome Analysis of the Developmental Effects of Bisphenol F Exposure in Chinese Medaka ( Oryzias sinensis). Int J Mol Sci 2023; 24:10898. [PMID: 37446079 DOI: 10.3390/ijms241310898] [Citation(s) in RCA: 2] [Impact Index Per Article: 1.0] [Reference Citation Analysis] [Abstract] [Key Words] [Grants] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 05/26/2023] [Revised: 06/27/2023] [Accepted: 06/28/2023] [Indexed: 07/15/2023] Open
Abstract
Bisphenol F (BPF) has been used in the syntheses of polymers, which are widely used in coatings, varnishes, adhesives, and other plastics. During the past decades, BPF contamination in the aquatic environment has dramatically increased due to its release from manmade products. Concerns have driven much attention to whether it may adversely impact aquatic lives or human beings. The present study performed an acute toxic exposure experiment and a 15 d developmental exposure of BPF at environmental concentrations (20, 200, and 2000 ng/L) using Chinese medaka (Oryzias sinensis). In the acute toxic exposure, the LC50 of BPF to Chinese medaka is 87.90 mg/L at 96 h. Developmental exposure induced a significant increase in the frequency of larvae with abnormalities in the 2000 ng/L BPF group compared to the control group. Transcriptomic analysis of the whole larvae revealed 565 up-regulated and 493 down-regulated genes in the 2000 ng/L BPF exposure group. Analysis of gene ontology and KEGG pathways enrichments indicated endocrine disorders to be associated with BPF-induced developmental toxicity. The present results suggest that BPF is developmentally toxic at 2000 ng/L concentration in Chinese medaka and causes endocrine-related aberrations in the transcriptional network of genes.
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Affiliation(s)
- Zhiying Liang
- Institute of Modern Aquaculture Science and Engineering, Guangdong-Macao Joint Laboratory for Aquaculture Breeding Development and Innovation, College of Life Sciences, South China Normal University, Guangzhou 510631, China
| | - Yafen Guo
- Institute of Modern Aquaculture Science and Engineering, Guangdong-Macao Joint Laboratory for Aquaculture Breeding Development and Innovation, College of Life Sciences, South China Normal University, Guangzhou 510631, China
| | - Duan Pi
- Institute of Modern Aquaculture Science and Engineering, Guangdong-Macao Joint Laboratory for Aquaculture Breeding Development and Innovation, College of Life Sciences, South China Normal University, Guangzhou 510631, China
| | - Xiang Li
- Institute of Modern Aquaculture Science and Engineering, Guangdong-Macao Joint Laboratory for Aquaculture Breeding Development and Innovation, College of Life Sciences, South China Normal University, Guangzhou 510631, China
| | - Bingying Li
- Institute of Modern Aquaculture Science and Engineering, Guangdong-Macao Joint Laboratory for Aquaculture Breeding Development and Innovation, College of Life Sciences, South China Normal University, Guangzhou 510631, China
| | - Yongsi Huang
- Institute of Modern Aquaculture Science and Engineering, Guangdong-Macao Joint Laboratory for Aquaculture Breeding Development and Innovation, College of Life Sciences, South China Normal University, Guangzhou 510631, China
| | - Xiaohong Song
- The Guangxi Key Laboratory of Theory and Technology for Environmental Pollution Control, Guilin University of Technology, Guilin 541000, China
| | - Ramji Kumar Bhandari
- Department of Biology, University of North Carolina Greensboro, Greensboro, NC 27412, USA
| | - Xuegeng Wang
- Institute of Modern Aquaculture Science and Engineering, Guangdong-Macao Joint Laboratory for Aquaculture Breeding Development and Innovation, College of Life Sciences, South China Normal University, Guangzhou 510631, China
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Mahmoudi A, Hadrich F, Bouallagui Z, Feki I, Ghorbel H, Ayadi L, Chamkha M, Sayadi S. Comparative study of the effect of oleuropein and hydroxytyrosol rich extracts on the reproductive toxicity induced by bisphenol A in male rats: biochemical, histopathological, and molecular analyses. ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH INTERNATIONAL 2023:10.1007/s11356-023-27836-y. [PMID: 37273043 DOI: 10.1007/s11356-023-27836-y] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Subscribe] [Scholar Register] [Received: 01/04/2023] [Accepted: 05/18/2023] [Indexed: 06/06/2023]
Abstract
Bisphenol A, or BPA, goes into the composition of a large number of products including sunglasses, infant's feeding bottles, receipts, or food packaging. Nowadays, there is a growing evidence that BPA may be at the origin of several physiological malignancies. Oleuropein and hydroxytyrosol extracted from olive leaves are highly investigated for numerous health benefits. The present work investigates the potential protective proprieties of olive leaf extracts against BPA-induced testicular damage in Wistar rats. Thirty-two animals were randomly divided into 4 groups: control, BPA-treated (10 mg/kg), BPA and oleuropein rich extract (16 mg/kg) treatment, and the last group treated with BPA and hydroxytyrosol rich extract (16 mg/kg). Biochemical parameters and histological and molecular analyses were evaluated. Our data demonstrated that BPA treatment caused significant alteration in biochemical parameters, disorganization of germinal epithelium, an up-regulation of p53 and Bax, and a reduction of Bcl-2 protein levels. The ingestion of oleuropein- and hydroxytyrosol-rich extracts attenuated BPA-induced biochemical and histological changes. In fact, olive leaf extracts enhanced the enzymatic antioxidant system and the level of Bcl-2, and reduced the expression of p53 and Bax. Fairly, our findings propose that olive leaf extracts may compete with BPA-induced reprotoxicity in vivo.
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Affiliation(s)
- Asma Mahmoudi
- Laboratory of Environmental Bioprocesses, Center of Biotechnology of Sfax, P.O. Box 1177, 3018, Sfax, Tunisia.
| | - Fatma Hadrich
- Laboratory of Environmental Bioprocesses, Center of Biotechnology of Sfax, P.O. Box 1177, 3018, Sfax, Tunisia
| | - Zouhaier Bouallagui
- Laboratory of Environmental Bioprocesses, Center of Biotechnology of Sfax, P.O. Box 1177, 3018, Sfax, Tunisia
| | - Ines Feki
- Laboratory of Environmental Bioprocesses, Center of Biotechnology of Sfax, P.O. Box 1177, 3018, Sfax, Tunisia
| | - Hela Ghorbel
- Higher Institute of Biotechnology, 1175, 3038, Sfax, BP, Tunisia
| | - Lobna Ayadi
- Laboratory of Pathology, CHU Habib Bourguiba Sfax, 3029, Sfax, Tunisia
| | - Mohamed Chamkha
- Laboratory of Environmental Bioprocesses, Center of Biotechnology of Sfax, P.O. Box 1177, 3018, Sfax, Tunisia
| | - Sami Sayadi
- Biotechnology Program, Center of Sustainable Development, College of Arts and Sciences, Qatar University, Doha, 2713, Qatar
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28
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Jenzri M, Gharred C, Bouraoui Z, Guerbej H, Jebali J, Gharred T. Assessment of single and combined effects of bisphenol-A and its analogue bisphenol-S on biochemical and histopathological responses of sea cucumber Holothuria poli. MARINE ENVIRONMENTAL RESEARCH 2023; 188:106032. [PMID: 37267666 DOI: 10.1016/j.marenvres.2023.106032] [Citation(s) in RCA: 2] [Impact Index Per Article: 1.0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Subscribe] [Scholar Register] [Received: 03/22/2023] [Revised: 05/21/2023] [Accepted: 05/23/2023] [Indexed: 06/04/2023]
Abstract
Bisphenols (BPs) are among emerging pollutants that have been frequently detected in different compartments of marine ecosystems and elicited great concern due to their potential toxicity to marine organisms. This work aimed to investigate the toxicity of bisphenol A (BPA) and bisphenol S (BPS) on oxidative stress markers, neurotoxicity and histopathological alterations in sea cucumbers (Holothuria poli). The results showed that exposure to 200 μg/L of BPA and BPS produced oxidative stress, neurotoxicity in the digestive tract and respiratory tree, and several types of histopathological lesions in tissues of the respiratory tree of the sea cucumber, posing a health hazard to this aquatic organism. In addition, BPA has greater effects than BPS on the generation of oxidative stress marked by the inductions of catalase (CAT), glutathione S-transferase (GST) and malondialdehyde (MDA) levels and neurotoxicity shown by the decrease in acetylcholinesterase activity (AChE). The respiratory tree of sea cucumbers might be an appropriate tissue for assessing CAT, MDA and AChE activity levels, which are reliable biomarkers that may be useful in marine biomonitoring studies. Evaluation of histopathological lesions of the respiratory tree suggests that BPA and BPS and their mixture cause various tissue alterations that may be associated with oxidative stress damage and neurotoxicity. In conclusion, this study showed that oxidative stress (CAT and MDA) and neurotoxicity (AChE) markers, as well as respiratory tree lesions, are sensitive biomarkers for the assessment of BPA and BPS toxicity in sea cucumbers.
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Affiliation(s)
- Maroua Jenzri
- Research Laboratory of Bioresources: Integrative Biology & Valorization (LR 14ES06), Higher Institute of Biotechnology of Monastir, Avenue Tahar Hadded, PB. 74, 5000 Monastir, Tunisia.
| | - Chayma Gharred
- Research Laboratory of Bioresources: Integrative Biology & Valorization (LR 14ES06), Higher Institute of Biotechnology of Monastir, Avenue Tahar Hadded, PB. 74, 5000 Monastir, Tunisia
| | - Zied Bouraoui
- Laboratory of Blue Biotechnology and Aquatic Bioproducts (B3Aqua), National Institute of Marine Sciences and Technology, BP 59, 5000, Monastir, Tunisia
| | - Hamadi Guerbej
- Laboratory of Blue Biotechnology and Aquatic Bioproducts (B3Aqua), National Institute of Marine Sciences and Technology, BP 59, 5000, Monastir, Tunisia
| | - Jamel Jebali
- Research Laboratory of Genetics, Biodiversity and Valorization of Bioresources (LR11ES41), Higher Institute of Biotechnology of Monastir, Avenue Tahar Hadded, PB. 74, 5000, Monastir, Tunisia
| | - Tahar Gharred
- Research Laboratory of Bioresources: Integrative Biology & Valorization (LR 14ES06), Higher Institute of Biotechnology of Monastir, Avenue Tahar Hadded, PB. 74, 5000 Monastir, Tunisia
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Rybczyńska-Tkaczyk K, Skóra B, Szychowski KA. Toxicity of bisphenol A (BPA) and its derivatives in divers biological models with the assessment of molecular mechanisms of toxicity. ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH INTERNATIONAL 2023:10.1007/s11356-023-27747-y. [PMID: 37213006 DOI: 10.1007/s11356-023-27747-y] [Citation(s) in RCA: 6] [Impact Index Per Article: 3.0] [Reference Citation Analysis] [Abstract] [Key Words] [Subscribe] [Scholar Register] [Received: 01/03/2023] [Accepted: 05/15/2023] [Indexed: 05/23/2023]
Abstract
The aim of the study was to determine totoxicity of bisphenol A (BPA) and its derivatives (bisphenol S (BPS), bisphenol F (BPF), and tetrabromobisphenol A (TBBPA)) due to its high accumulation in environment. The performed analysis revealed the toxicity of the BPA, BPF, and BPS against Kurthia gibsoni, Microbacterium sp., and Brevundimonas diminuta as the most sensitive, reaching microbial toxic concentrations in the range of 0.018-0.031 mg ∙ L-1. Moreover, the genotoxicity assay shows the ability of all tested compounds to increase in the β-galactosidase level at the concentration range 7.81-500 µM (in Escherichia coli, PQ37). In turn, the matbolic activation of tested bishpenols has caused the enhacement of the genotoxicity and cytotoxicity effect. Interestingely, the highest phytotoxicity effect was pointed for BPA and TBBPA at the concentrations of 10 mg ∙ L-1 and 50 mg ∙ L-1, which cause the inhibition of root growth by 58% and 45%, respectively (especially for S. alba and S. saccharatum). Furthermore, the cytotoxicity analyses show the ability of BPA, BPS, and TBBPA to significantly decrease the metabolic activity of human keratynoctes in vitro after 24 h of treatment at the micromolar concentrations. Simialry, the impact of the certain bisphenols on proliferation-, apoptosis-, and inflammation-related mRNA expression was shown in tested cell line. Summarizing, the presented results have proved that BPA and its derrivatives are able to show high negative effect on certain living orgnisms such as bacteria, plants, and human cells, which is strict related to pro-apoptotic and genotoxic mechanism of action.
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Affiliation(s)
- Kamila Rybczyńska-Tkaczyk
- Department of Environmental Microbiology, The University of Life Sciences, Leszczyńskiego Street 7, 20-069, Lublin, Poland
| | - Bartosz Skóra
- Department of Biotechnology and Cell Biology, Medical College, University of Information Technology and Management in Rzeszow, Sucharskiego 2, 35-225, Rzeszow, Poland
| | - Konrad A Szychowski
- Department of Biotechnology and Cell Biology, Medical College, University of Information Technology and Management in Rzeszow, Sucharskiego 2, 35-225, Rzeszow, Poland.
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Yuan M, Chen S, Zeng C, Fan Y, Ge W, Chen W. Estrogenic and non-estrogenic effects of bisphenol A and its action mechanism in the zebrafish model: An overview of the past two decades of work. ENVIRONMENT INTERNATIONAL 2023; 176:107976. [PMID: 37236126 DOI: 10.1016/j.envint.2023.107976] [Citation(s) in RCA: 14] [Impact Index Per Article: 7.0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Subscribe] [Scholar Register] [Received: 02/26/2023] [Revised: 04/11/2023] [Accepted: 05/10/2023] [Indexed: 05/28/2023]
Abstract
Bisphenol A (BPA) is the most simple and predominant component of the Bisphenol family. BPA is widely present in the environment and the human body as a result of its extensive usage in the plastic and epoxy resins of consumer goods like water bottles, food containers, and tableware. Since the 1930s, when BPA's estrogenic activity was first observed, and it was labeled as a "mimic hormone of E2", studies on the endocrine-disrupting effects of BPA then have been widely conducted. As a top vertebrate model for genetic and developmental studies, the zebrafish has caught tremendous attention in the past two decades. By using the zebrafish, the negative effects of BPA either through estrogenic signaling pathways or non-estrogenic signaling pathways were largely found. In this review, we tried to draw a full picture of the current state of knowledge on the estrogenic and non-estrogenic effects of BPA with their mechanisms of action through the zebrafish model of the past two decades, which may help to fully understand the endocrine-disrupting effects of BPA and its action mechanism, and give a direction for the future studies.
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Affiliation(s)
- Mingzhe Yuan
- Department of Biomedical Sciences and Centre of Reproduction, Development and Aging (CRDA), Faculty of Health Sciences, University of Macau, Taipa, Macau, China
| | - Shan Chen
- State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, School of Marine Sciences, Ningbo University, Ningbo 315211, China
| | - Chu Zeng
- Department of Biomedical Sciences and Centre of Reproduction, Development and Aging (CRDA), Faculty of Health Sciences, University of Macau, Taipa, Macau, China
| | - Yuqin Fan
- Guangdong Provincial Key Laboratory of Conservation and Precision Utilization of Characteristic Agricultural Resources in Mountainous Area, School of Life Sciences, Jiaying University, Meizhou 514015, China
| | - Wei Ge
- Department of Biomedical Sciences and Centre of Reproduction, Development and Aging (CRDA), Faculty of Health Sciences, University of Macau, Taipa, Macau, China.
| | - Weiting Chen
- Guangdong Provincial Key Laboratory of Conservation and Precision Utilization of Characteristic Agricultural Resources in Mountainous Area, School of Life Sciences, Jiaying University, Meizhou 514015, China.
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Abouhamzeh B, Zare Z, Mohammadi M, Moosazadeh M, Nourian A. Bisphenol-S Influence on Oxidative Stress and Endocrine Biomarkers of Reproductive System: A Systematic Review and Meta-Analysis. Int J Prev Med 2023; 14:37. [PMID: 37351052 PMCID: PMC10284209 DOI: 10.4103/ijpvm.ijpvm_271_21] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 06/15/2021] [Accepted: 10/27/2022] [Indexed: 06/24/2023] Open
Abstract
Background Bisphenol-S (BPS), as a new human public health concern, was introduced to the plastic industry by BPA-free labeled products following the restrictions of Bisphenol-A (BPA) as a safe alternative. However, recent research has revealed a controversial issue. In this regard, the present study aimed to review the relationship between BPS exposure and reproductive system dis/malfunction. Methods PubMed and other databases were searched up to January 2021. The standard mean difference (SMD) with a 95% confidence interval (CI) was calculated for the main parameters using the random-effects model. Finally, 12 studies with 420 subjects were included in this research. Forest plot, meta-regression, and non-linear dose-response effect were calculated for each parameter by random-effects model. Results Based on the results of in vitro assessment, a significant increase was found in the oxidative stress parameters, including superoxide dismutase (SMD: 0.63, 95% CI: 0.321, 0.939), thiobarbituric acid reactive substances (SMD: 0.760, 95% CI: 0.423, 1.096), and reactive oxygen species (SMD: 0.484, 95% CI: 0.132, 0.835). In addition, the hormonal assessment revealed a significant decrease in male testosterone concertation (SMD: -0.476, 95% CI: -0.881, -0.071). Moreover, in vivo examination revealed a significant decrease in hormonal parameters, such as female testosterone (SMD: -0.808, 95% CI: -1.149, -0.467), female estrogen (SMD: -2.608, 95% CI: -4.588, -0.628), female luteinizing hormone (SMD: -0.386, 95% CI: -0.682, -0.089), and female follicle-stimulating hormone (FSH) (SMD: -0.418, 95% CI: -0.716, -0.119). Besides, linear and non-linear correlations were detected in the main parameters. Conclusion In conclusion, based on the current meta-analysis, BPS was suggested to be toxic for the reproductive system, similar to the other bisphenols. Moreover, a possible correlation was indicated between oxidative and hormonal status disruption induced by BPS in male and female reproductive systems dis/malfunction.
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Affiliation(s)
- Beheshteh Abouhamzeh
- Alireza Nourian Department of Anatomical Sciences, School of Medicine, AJA University of Medical Sciences, Tehran, Iran
| | - Zohreh Zare
- Department of Anatomical Sciences, Molecular and Cell Biology Research Center, School of Medicine, Sari, Iran
| | - Moslem Mohammadi
- Department of Physiology, Molecular and Cell Biology Research Center, School of Medicine, Mazandaran University of Medical Sciences, Sari, Iran
| | - Mahmood Moosazadeh
- Department of Epidemiology, Gastrointestinal Cancer Research Center, Non-communicable Diseases Institute, Mazandaran University of Medical Sciences, Sari, Iran
| | - Alireza Nourian
- Alireza Nourian Department of Anatomical Sciences, School of Medicine, AJA University of Medical Sciences, Tehran, Iran
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Zhang Y, Liu J, Jing C, Lu G, Jiang R, Zheng X, He C, Ji W. Life history traits of low-toxicity alternative bisphenol S on Daphnia magna with short breeding cycles: A multigenerational study. ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY 2023; 253:114682. [PMID: 36842276 DOI: 10.1016/j.ecoenv.2023.114682] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Subscribe] [Scholar Register] [Received: 11/30/2022] [Revised: 02/15/2023] [Accepted: 02/21/2023] [Indexed: 06/18/2023]
Abstract
Due to relatively lower toxicity, bisphenol S (BPS) has become an alternative to previously used bisphenol A. Nevertheless, the occurrence of BPS and its ecological impact have recently attracted increasing attentions because the toxicology effect of BPS with life cycle or multigenerational exposure on aquatic organisms remains questionable. Herein, Daphnia magna (D. magna) multigenerational bioassays spanning four generations (F0-F3) and single-generation recovery (F1 and F3) in clean water were used to investigate the ecotoxicology of variable chronic BPS exposure. For both assays, four kinds of life-history traits (i.e., survival, reproduction, growth and ecological behavior) were examined for each generation. After an 18-day exposure under concentration of 200 μg/L, the survival rate of D. magna was less than 15 % for the F2 generation, whereas all died for the F3 generation. With continuous exposure of four generations of D. magna at environmentally relevant concentrations of BPS (2 μg/L), inhibition of growth and development, prolonged sexual maturity, decreased offspring production and decreased swimming activity were observed for the F3 generation. In particular, it is difficult for D. magna to return to its normal level through a single-generation recovery in clean water in terms of reproductive function, ecological behavior and population health. Hence, multi-generational exposure to low concentrations of BPS can have adverse effects on population health of aquatic organisms with short breeding cycles, highlighting the necessity to assess the ecotoxicology of chronic BPS exposure for public health.
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Affiliation(s)
- Yixuan Zhang
- Key Laboratory of Integrated Regulation and Resources Development of Shallow Lakes of Ministry of Education, Hohai University, Nanjing 210098, China; College of Environment, Hohai University, Nanjing 210098, China
| | - Jianchao Liu
- Key Laboratory of Integrated Regulation and Resources Development of Shallow Lakes of Ministry of Education, Hohai University, Nanjing 210098, China; College of Environment, Hohai University, Nanjing 210098, China.
| | - Chenyang Jing
- Key Laboratory of Integrated Regulation and Resources Development of Shallow Lakes of Ministry of Education, Hohai University, Nanjing 210098, China; College of Environment, Hohai University, Nanjing 210098, China
| | - Guanghua Lu
- Key Laboratory of Integrated Regulation and Resources Development of Shallow Lakes of Ministry of Education, Hohai University, Nanjing 210098, China; College of Environment, Hohai University, Nanjing 210098, China
| | - Runren Jiang
- Key Laboratory of Integrated Regulation and Resources Development of Shallow Lakes of Ministry of Education, Hohai University, Nanjing 210098, China; College of Environment, Hohai University, Nanjing 210098, China
| | - Xiqiang Zheng
- Key Laboratory of Integrated Regulation and Resources Development of Shallow Lakes of Ministry of Education, Hohai University, Nanjing 210098, China; College of Environment, Hohai University, Nanjing 210098, China; Anhui Academy of Ecological and Environmental Sciences, Key Laboratory of Wastewater Treatment Technology in Anhui Province, Hefei 230061, China
| | - Chao He
- Faculty of Engineering and Natural Sciences, Tampere University, Tampere, Finland
| | - Wenliang Ji
- Jiangsu Provincial Center for Disease Control and Prevention, Nanjing 210009, China.
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Pahović PŠ, Iulini M, Maddalon A, Galbiati V, Buoso E, Dolenc MS, Corsini E. In Vitro Effects of Bisphenol Analogs on Immune Cells Activation and Th Differentiation. Endocr Metab Immune Disord Drug Targets 2023; 23:1750-1761. [PMID: 36797609 DOI: 10.2174/1871530323666230216150614] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Grants] [Track Full Text] [Journal Information] [Submit a Manuscript] [Subscribe] [Scholar Register] [Received: 07/11/2022] [Revised: 12/01/2022] [Accepted: 12/20/2022] [Indexed: 02/18/2023]
Abstract
AIMS Investigate the immunomodulatory effects of bisphenols in the THP-1 cell line and peripheral blood mononuclear cells in response to lipopolysaccharide (LPS) activation or to phorbol 12-myristate 13-acetate (PMA) and ionomycin. BACKGROUND We have previously demonstrated the usefulness of the evaluation of RACK1 expression as a link between endocrine disrupting activity and the immunotoxic effect of xenobiotics. We demonstrated that while BPA and BPAF reduced RACK1 expression, BPS was able to increase it. OBJECTIVE Bisphenol A (BPA) is one of the most commonly used chemicals in the manufacturing of polycarbonate plastics and plastic consumer products. Its endocrine disrupting (ED) potential and changes in European regulations have led to replacing BPA in many uses with structurally similar chemicals, like bisphenol AF (BPAF) and bisphenol S (BPS). However, emerging data indicated that bisphenol analogues may not be safer than BPA both in toxic effects and ED potential. METHODS THP-1 cell line and peripheral blood mononuclear cells were activated with lipopolysaccharide (LPS) or with phorbol 12-myristate 13-acetate (PMA) and ionomycin. RESULTS BPA and BPAF decreased LPS-induced expression of surface markers and the release of pro-inflammatory cytokines, while BPS increased LPS-induced expression of CD86 and cytokines. BPA, BPAF, and BPS affected PMA/ionomycin-induced T helper differentiation and cytokine release with gender-related alterations in some parameters investigated. CONCLUSION Data confirm that bisphenols can modulate immune cell differentiation and activation, further supporting their immunotoxic effects.
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Affiliation(s)
- Pia Štrukelj Pahović
- Faculty of Pharmacy, University of Ljubljana, Ljubljana, Slovenia
- Department of Pharmacological and Biomolecular Sciences, Laboratory of Toxicology, Università degli Studi di Milano, Milan, Italy
| | - Martina Iulini
- Department of Pharmacological and Biomolecular Sciences, Laboratory of Toxicology, Università degli Studi di Milano, Milan, Italy
| | - Ambra Maddalon
- Department of Pharmacological and Biomolecular Sciences, Laboratory of Toxicology, Università degli Studi di Milano, Milan, Italy
| | - Valentina Galbiati
- Department of Pharmacological and Biomolecular Sciences, Laboratory of Toxicology, Università degli Studi di Milano, Milan, Italy
| | - Erica Buoso
- Department of Drugs Sciences, University of Pavia, Pavia, Italy
| | | | - Emanuela Corsini
- Department of Pharmacological and Biomolecular Sciences, Laboratory of Toxicology, Università degli Studi di Milano, Milan, Italy
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Wang H, Qi S, Mu X, Yuan L, Li Y, Qiu J. Bisphenol F induces liver-gut alteration in zebrafish. THE SCIENCE OF THE TOTAL ENVIRONMENT 2022; 851:157974. [PMID: 35963407 DOI: 10.1016/j.scitotenv.2022.157974] [Citation(s) in RCA: 9] [Impact Index Per Article: 3.0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Subscribe] [Scholar Register] [Received: 05/02/2022] [Revised: 07/23/2022] [Accepted: 08/07/2022] [Indexed: 06/15/2023]
Abstract
The unease of consumers with bisphenol A has led to the increased industrial usage of bisphenol F (BPF), which is a new hazard to environmental health. Here, zebrafish were exposed to three BPF concentrations (0.5, 5, and 50 μg/L) from the embryonic stage for 180 days. Results showed that zebrafish body length and weight decreased and hepatosomatic index values increased, even at environmentally relevant concentration. Histological analysis identified the occurrence of hepatic fibrosis and steatosis in 5 and 50 μg/L groups, which indicated the liver injury caused by BPF. Based on the untargeted metabolomics results, a dose-dependent variation in the effects of BPF on liver metabolism was found, and amino acids, purines and one carbon metabolism were the main affected processes in the 0.5, 5, and 50 μg/L treatments, respectively. At the same time, BPF induced a shift in intestinal microbiome composition, including decreased abundance of Erysipelotrichaceae, Rhodobacteraceae and Gemmobacter. In addition, the correlation analysis suggested an association between gut microbiome changes and affected hepatic metabolites after BPF exposure. These findings indicate that a liver-gut alteration is induced by long-term BPF exposure.
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Affiliation(s)
- Hui Wang
- Institute of Quality Standard and Testing Technology for Agro-Products, Chinese Academy of Agricultural Sciences, Beijing, China; Fishery Resource and Environment Research Center, Chinese Academy of Fishery Sciences, Beijing, China
| | - Suzhen Qi
- Institute of Apicultural Research, Chinese Academy of Agricultural Sciences, Beijing, China
| | - Xiyan Mu
- Institute of Quality Standard and Testing Technology for Agro-Products, Chinese Academy of Agricultural Sciences, Beijing, China; Fishery Resource and Environment Research Center, Chinese Academy of Fishery Sciences, Beijing, China.
| | - Lilai Yuan
- Fishery Resource and Environment Research Center, Chinese Academy of Fishery Sciences, Beijing, China
| | - Yingren Li
- Fishery Resource and Environment Research Center, Chinese Academy of Fishery Sciences, Beijing, China
| | - Jing Qiu
- Institute of Quality Standard and Testing Technology for Agro-Products, Chinese Academy of Agricultural Sciences, Beijing, China.
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Li X, Zhao B, Luo L, Zhou Y, Lai D, Luan T. In vitro immunotoxicity detection for environmental pollutants: Current techniques and future perspectives. Trends Analyt Chem 2022. [DOI: 10.1016/j.trac.2022.116901] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 12/29/2022]
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Comparison of the cytotoxicity of bisphenol A and its analogs on human placental BeWo cells. Mol Cell Toxicol 2022. [DOI: 10.1007/s13273-022-00312-3] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 11/20/2022]
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Moreno-Gómez-Toledano R. Relationship between emergent BPA-substitutes and renal and cardiovascular diseases in adult population. ENVIRONMENTAL POLLUTION (BARKING, ESSEX : 1987) 2022; 313:120106. [PMID: 36084738 DOI: 10.1016/j.envpol.2022.120106] [Citation(s) in RCA: 14] [Impact Index Per Article: 4.7] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Subscribe] [Scholar Register] [Received: 05/26/2022] [Revised: 08/11/2022] [Accepted: 09/01/2022] [Indexed: 05/26/2023]
Abstract
Plastic waste pollution is one of the leading environmental problems of modern society. Its use, disposal, and recycling lead to the release of xenobiotic compounds such as bisphenol A (BPA), a known endocrine disruptor related to numerous pathologies. Due to the new restrictions on its use, it is gradually being replaced by derived molecules, such as bisphenol F or S (BPF or BPS), whose health risks have not yet been adequately studied. In the present work, significant relationships between the new BPA substitute molecules and renal and cardiovascular diseases have been detected by performing binomial and multinomial logistic regressions in one of the world's largest cohorts of urinary phenols. The results have shown a significant relationship between urinary BPF and renal function or heart disease (specifically congestive heart failure). Urinary BPS has shown a positive relationship with the risk of hypertension and a negative relationship with kidney disease. Consequently, applying new substitute molecules could imply potential health risks equivalent to BPA.
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Gu J, Zhu Y, Guo M, Yin X, Liang M, Lou X, Chen J, Zhou L, Fan D, Shi L, Hu G, Ji G. The potential mechanism of BPF-induced neurotoxicity in adult zebrafish: Correlation between untargeted metabolomics and gut microbiota. THE SCIENCE OF THE TOTAL ENVIRONMENT 2022; 839:156221. [PMID: 35623532 DOI: 10.1016/j.scitotenv.2022.156221] [Citation(s) in RCA: 21] [Impact Index Per Article: 7.0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Subscribe] [Scholar Register] [Received: 03/14/2022] [Revised: 05/11/2022] [Accepted: 05/21/2022] [Indexed: 06/15/2023]
Abstract
Bisphenol F (BPF) is becoming the main substitute for bisphenol A (BPA) in plastics for food and beverage applications. Previous studies have demonstrated the neurotoxicity of BPF; however, its lifecycle toxicity and the underlying mechanisms remain poorly understood. In the current study, zebrafish were continuously exposed to BPF for four months from the embryo to adult stages in order to assess its neurotoxicity. Locomotor behaviors significantly decreased after BPF exposure, which was accompanied by a decrease in body weight, length, and hatching rate. Additionally, BPF increased the expression of inflammatory genes in the brain and destroyed the zebrafishes' intestinal integrity. Meanwhile, the 16S rRNA gene sequence results showed a significantly decreased microbiota abundance and diversity following BPF treatment. Neurotransmitter metabolites were also altered by BPF. Notably, the correlation analysis between microbiota and neurotransmitter metabolism verified that gut microbiota dysbiosis was closely related to the disturbance of neurotransmitter metabolites. Therefore, the present study evaluated the neurotoxicity of lifecycle exposure to BPF and unraveled a novel mechanism involving disturbance of neurotransmitter metabolism and gut dysbiosis, which may provide potential targets for BPF-mediated neurotoxicity.
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Affiliation(s)
- Jie Gu
- Nanjing Institute of Environmental Sciences, Ministry of Ecology and Environment, Nanjing 210042, China
| | - Yuanhui Zhu
- Key Laboratory of Modern Toxicology, Ministry of Education, School of Public Health, Nanjing Medical University, Nanjing 211166, China
| | - Min Guo
- Nanjing Institute of Environmental Sciences, Ministry of Ecology and Environment, Nanjing 210042, China
| | - Xiaogang Yin
- Nanjing Institute of Environmental Sciences, Ministry of Ecology and Environment, Nanjing 210042, China
| | - Mengyuan Liang
- Nanjing Institute of Environmental Sciences, Ministry of Ecology and Environment, Nanjing 210042, China
| | - Xinyu Lou
- Key Laboratory of Modern Toxicology, Ministry of Education, School of Public Health, Nanjing Medical University, Nanjing 211166, China
| | - Jingrong Chen
- Key Laboratory of Modern Toxicology, Ministry of Education, School of Public Health, Nanjing Medical University, Nanjing 211166, China
| | - Linjun Zhou
- Nanjing Institute of Environmental Sciences, Ministry of Ecology and Environment, Nanjing 210042, China
| | - Deling Fan
- Nanjing Institute of Environmental Sciences, Ministry of Ecology and Environment, Nanjing 210042, China
| | - Lili Shi
- Nanjing Institute of Environmental Sciences, Ministry of Ecology and Environment, Nanjing 210042, China
| | - Guocheng Hu
- State Environmental Protection Key Laboratory of Environmental Pollution Health Risk Assessment, Center for Environmental Health Research, South China Institute of Environmental Sciences,Ministry of Ecology and Environment, Guangzhou 510655, China.
| | - Guixiang Ji
- Nanjing Institute of Environmental Sciences, Ministry of Ecology and Environment, Nanjing 210042, China.
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Adaptation and Resistance: How Bacteroides thetaiotaomicron Copes with the Bisphenol A Substitute Bisphenol F. Microorganisms 2022; 10:microorganisms10081610. [PMID: 36014027 PMCID: PMC9414779 DOI: 10.3390/microorganisms10081610] [Citation(s) in RCA: 2] [Impact Index Per Article: 0.7] [Reference Citation Analysis] [Abstract] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 07/14/2022] [Revised: 08/03/2022] [Accepted: 08/04/2022] [Indexed: 11/17/2022] Open
Abstract
Bisphenols are used in the process of polymerization of polycarbonate plastics and epoxy resins. Bisphenols can easily migrate out of plastic products and enter the gastrointestinal system. By increasing colonic inflammation in mice, disrupting the intestinal bacterial community structure and altering the microbial membrane transport system in zebrafish, bisphenols seem to interfere with the gut microbiome. The highly abundant human commensal bacterium Bacteroides thetaiotaomicron was exposed to bisphenols (Bisphenol A (BPA), Bisphenol F (BPF), Bisphenol S (BPS)), to examine the mode of action, in particular of BPF. All chemicals caused a concentration-dependent growth inhibition and the half-maximal effective concentration (EC50) corresponded to their individual logP values, a measure of their hydrophobicity. B. thetaiotaomicron exposed to BPF decreased membrane fluidity with increasing BPF concentrations. Physiological changes including an increase of acetate concentrations were observed. On the proteome level, a higher abundance of several ATP synthase subunits and multidrug efflux pumps suggested an increased energy demand for adaptive mechanisms after BPF exposure. Defense mechanisms were also implicated by a pathway analysis that identified a higher abundance of members of resistance pathways/strategies to cope with xenobiotics (i.e., antibiotics). Here, we present further insights into the mode of action of bisphenols in a human commensal gut bacterium regarding growth inhibition, and the physiological and functional state of the cell. These results, combined with microbiota-directed effects, could lead to a better understanding of host health disturbances and disease development based on xenobiotic uptake.
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Benjamin K, Marquez CM, Morta M, Reyes EM, Aragones L, Velarde M. Bisphenol S Increases Cell Number and Stimulates Migration of Endometrial Epithelial Cells. J ASEAN Fed Endocr Soc 2022; 38:13-22. [PMID: 37234927 PMCID: PMC10207871 DOI: 10.15605/jafes.037.s7] [Citation(s) in RCA: 2] [Impact Index Per Article: 0.7] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 04/15/2022] [Accepted: 06/13/2022] [Indexed: 09/08/2023] Open
Abstract
OBJECTIVE To determine whether bisphenol S (BPS), a common substitute for bisphenol A (BPA), induces cell proliferation and migration in human endometrial epithelial cells (Ishikawa) and adult mouse uterine tissues. METHODOLOGY Human endometrial Ishikawa cells were exposed to low doses of BPS (1 nM and 100 nM) for 72 hours. Cell proliferation was assessed through the viability assays MTT and CellTiter-Glo®. Wound healing assays were also used to evaluate the migration potential of the cell line. The expression of genes related to proliferation and migration was also determined. Similarly, adult mice were exposed to BPS at a dose of 30 mg/kg body weight/day for 21 days, after which, the uterus was sent for histopathologic assessment. RESULTS BPS increased cell number and stimulated migration in Ishikawa cells, in association with the upregulation of estrogen receptor beta (ESR2) and vimentin (VIM). In addition, mice exposed to BPS showed a significantly higher mean number of endometrial glands within the endometrium. CONCLUSION Overall, in vitro and in vivo results obtained in this study showed that BPS could significantly promote endometrial epithelial cell proliferation and migration, a phenotype also observed with BPA exposure. Hence, the use of BPS in BPA-free products must be reassessed, as it may pose adverse reproductive health effects to humans.
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Affiliation(s)
- Kimberly Benjamin
- Department of Biology, College of Arts and Sciences, University of the Philippines Manila
- Institute of Environmental Science and Meteorology, College of Science, University of the Philippines Diliman, Quezon City, Philippines
| | - Cielo Mae Marquez
- Institute of Biology, College of Science, University of the Philippines Diliman, Quezon City, Philippines
| | - Madeleine Morta
- Institute of Biology, College of Science, University of the Philippines Diliman, Quezon City, Philippines
| | - Emmanuel Marc Reyes
- Institute of Biology, College of Science, University of the Philippines Diliman, Quezon City, Philippines
| | - Lemnuel Aragones
- Institute of Environmental Science and Meteorology, College of Science, University of the Philippines Diliman, Quezon City, Philippines
| | - Michael Velarde
- Institute of Biology, College of Science, University of the Philippines Diliman, Quezon City, Philippines
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Moreno-Gómez-Toledano R, Vélez-Vélez E, Arenas MI, Saura M, Bosch RJ. Association between urinary concentrations of bisphenol A substitutes and diabetes in adults. World J Diabetes 2022; 13:521-531. [PMID: 36051427 PMCID: PMC9329846 DOI: 10.4239/wjd.v13.i7.521] [Citation(s) in RCA: 6] [Impact Index Per Article: 2.0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Journal Information] [Submit a Manuscript] [Subscribe] [Scholar Register] [Received: 03/11/2022] [Revised: 04/26/2022] [Accepted: 06/22/2022] [Indexed: 02/06/2023] Open
Abstract
BACKGROUND Due to new restrictions on the use of bisphenol A (BPA), industries are beginning to replace it with derived molecules such as bisphenol S and F (BPS and BPF). There is extensive evidence in the academic literature on the potential health effects of BPA, which is known to be a diabetogenic molecule. However, there are few publications related to new compounds derived from BPA. AIM To perform an epidemiological study of urinary BPS and BPF in the American National Health and Nutrition Examination Survey (NHANES) cohort, and analyze their possible relationship with diabetes mellitus. METHODS NHANES datasets from 2013 to 2016 were used due to the urinary BPF and BPS availability. Data from 3658 adults were analyzed to perform regression analysis exploring the possible relationship between BPA-derived compounds and diabetes. RESULTS Descriptive statistics, linear regression modeling, and logistic regression analysis revealed a significant relationship between urinary BPS, but not BPF, and diabetes risk. Additionally, a relationship was observed between both compounds and hypertension and a slight relationship between BPF and dyslipidemia. CONCLUSION In the present study, a strong relationship between urinary BPS, not BPF, and diabetes risk has been determined. BPA substitute molecules do not exempt the population from potential health risks.
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Affiliation(s)
| | - Esperanza Vélez-Vélez
- Fundación Jiménez Díaz School of Nursing, Jiménez Díaz Foundation, Autonomous University of Madrid, Madrid 28040, Spain
| | - María I Arenas
- Universidad de Alcalá, Department of Biomedicine and Biotechnology,Alcalá de Henares 28871, Spain
| | - Marta Saura
- Universidad de Alcalá, Department of Biological Systems/Physiology Unit, Alcalá de Henares 28871, Spain
- Centro de Investigación en Red de Enfermedades Cardiovasculares, Instituto Ramón y Cajal de Investigación Sanitaria, Madrid 28034, Spain
| | - Ricardo J Bosch
- Universidad de Alcalá, Department of Biological Systems/Physiology Unit, Alcalá de Henares 28871, Spain
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Salahinejad A, Attaran A, Meuthen D, Rachamalla M, Chivers DP, Niyogi S. Maternal exposure to bisphenol S induces neuropeptide signaling dysfunction and oxidative stress in the brain, and abnormal social behaviors in zebrafish (Danio rerio) offspring. THE SCIENCE OF THE TOTAL ENVIRONMENT 2022; 830:154794. [PMID: 35341835 DOI: 10.1016/j.scitotenv.2022.154794] [Citation(s) in RCA: 9] [Impact Index Per Article: 3.0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Subscribe] [Scholar Register] [Received: 11/11/2021] [Revised: 03/16/2022] [Accepted: 03/20/2022] [Indexed: 06/14/2023]
Abstract
Recent studies show that bisphenol S (BPS) induces multiple adverse effects in exposed organisms; however, the maternal effects of BPS exposure remain poorly understood. Here, we expose adult female zebrafish to environmentally relevant concentrations of BPS (0, 1, 10, 30 μg/L) and 1 μg/L of 17-β-estradiol (E2) as a positive control for 60 days. Females were then paired with BPS-unexposed males and their offspring were raised in control water for 6 months. Maternal exposure to BPS was found to alter social behavior and anxiety response in a dose-specific manner in male offspring. Group preferences and social cohesion were significantly reduced by maternal exposure to 1 and 10 μg/L BPS, respectively. Additionally, maternal exposure to 1 and 30 μg/L BPS and E2 decreased offspring stress responses during the novel tank test. The impaired social behavior was associated with elevated arginine-vasotocin (AVT) level as well as with the altered expression of genes involved in AVT signaling pathway (AVT, avpr1aa) and enzymatic antioxidant genes (cat and Mn-sod) in the brain. Collectively, these results suggest that maternal exposure to environmentally relevant concentrations of BPS alters social behavior in zebrafish offspring, which is likely mediated by oxidative stress and disruption of neuropeptide signaling pathways in the brain.
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Affiliation(s)
- Arash Salahinejad
- Department of Biology, University of Saskatchewan, Saskatoon, SK S7N 5E2, Canada.
| | - Anoosha Attaran
- Department of Biology, University of Saskatchewan, Saskatoon, SK S7N 5E2, Canada
| | - Denis Meuthen
- Department of Biology, University of Saskatchewan, Saskatoon, SK S7N 5E2, Canada; Evolutionary Biology, Bielefeld University, 33615 Bielefeld, Germany
| | - Mahesh Rachamalla
- Department of Biology, University of Saskatchewan, Saskatoon, SK S7N 5E2, Canada
| | - Douglas P Chivers
- Department of Biology, University of Saskatchewan, Saskatoon, SK S7N 5E2, Canada
| | - Som Niyogi
- Department of Biology, University of Saskatchewan, Saskatoon, SK S7N 5E2, Canada; Toxicology Centre, University of Saskatchewan, 44 Campus Drive, Saskatoon, SK S7N 5B3, Canada
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Gu J, Li L, Yin X, Liang M, Zhu Y, Guo M, Zhou L, Fan D, Shi L, Ji G. Long-term exposure of zebrafish to bisphenol F: Adverse effects on parental reproduction and offspring neurodevelopment. AQUATIC TOXICOLOGY (AMSTERDAM, NETHERLANDS) 2022; 248:106190. [PMID: 35561629 DOI: 10.1016/j.aquatox.2022.106190] [Citation(s) in RCA: 14] [Impact Index Per Article: 4.7] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Subscribe] [Scholar Register] [Received: 12/28/2021] [Revised: 04/01/2022] [Accepted: 05/04/2022] [Indexed: 06/15/2023]
Abstract
Bisphenol F (BPF), an alternative to bisphenol A (BPA) has potential endocrine and reproductive toxicity; however, the effects of environmental concentrations of BPF on the reproductive and developmental toxicity of offspring following parental exposure to BPF remain unclear. In the present study, the effects of life-cycle BPF exposure at environmental concentrations on zebrafish reproduction, offspring growth, and development were investigated. The results showed that the life-cycle of BPF exposure significantly elevated oxidative stress levels, increased gonadal apoptosis, and reduced zebrafish (F0) spawning. Notably, through maternal transfer, BPF exposure significantly affected offspring development. Developmental parameters such as hatching rate, spontaneous movements, heart rate, body length, and locomotor behavior decreased in zebrafish larvae (F1). In addition, the expression levels of genes related to oxidative stress, apoptosis, and neurodevelopment were altered in F1 larvae. Therefore, the present study provides evidence that BPF, even at environmental concentrations, can be potentially adverse in terms of reproductive defects and offspring neurodevelopmental disorders. Therefore, BPF, as a substitute for BPA, is worthy of in-depth evaluation.
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Affiliation(s)
- Jie Gu
- Nanjing Institute of Environmental Sciences, Ministry of Ecology and Environment, Nanjing 210042, China
| | - Liangzhong Li
- State Environmental Protection Key Laboratory of Environmental Pollution Health Risk Assessment, Center for Environmental Health Research, South China Institute of Environmental Sciences, Ministry of Ecology and Environment, Guangzhou, 510655, China
| | - Xiaogang Yin
- Nanjing Institute of Environmental Sciences, Ministry of Ecology and Environment, Nanjing 210042, China
| | - Mengyuan Liang
- Nanjing Institute of Environmental Sciences, Ministry of Ecology and Environment, Nanjing 210042, China
| | - Yuanhui Zhu
- Nanjing Institute of Environmental Sciences, Ministry of Ecology and Environment, Nanjing 210042, China
| | - Min Guo
- Nanjing Institute of Environmental Sciences, Ministry of Ecology and Environment, Nanjing 210042, China
| | - Linjun Zhou
- Nanjing Institute of Environmental Sciences, Ministry of Ecology and Environment, Nanjing 210042, China
| | - Deling Fan
- Nanjing Institute of Environmental Sciences, Ministry of Ecology and Environment, Nanjing 210042, China
| | - Lili Shi
- Nanjing Institute of Environmental Sciences, Ministry of Ecology and Environment, Nanjing 210042, China
| | - Guixiang Ji
- Nanjing Institute of Environmental Sciences, Ministry of Ecology and Environment, Nanjing 210042, China.
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Dos Santos B, Ivantsova E, Guzman AP, Martyniuk CJ. Critical review of the toxicity mechanisms of bisphenol F in zebrafish (Danio rerio): Knowledge gaps and future directions. CHEMOSPHERE 2022; 297:134132. [PMID: 35240145 DOI: 10.1016/j.chemosphere.2022.134132] [Citation(s) in RCA: 15] [Impact Index Per Article: 5.0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Subscribe] [Scholar Register] [Received: 11/25/2021] [Revised: 02/22/2022] [Accepted: 02/24/2022] [Indexed: 06/14/2023]
Abstract
Replacement chemicals for bisphenol A, such as bisphenol F (BPF), are detected in aquatic environments worldwide and can potentially exert negative effects on aquatic organisms. We synthesized peer-reviewed literature reporting molecular and physiological responses in zebrafish following exposure to BPF, as BPF is closely related to BPA structure and is a dominant replacement chemical in the marketplace. Global concentrations of BPF in aquatic environments were compiled and compared to physiological and behavioral impacts reported in zebrafish (e.g., developmental abnormalities, oxidative stress, immunotoxicity, endocrine disruption, and neurotoxicity). Using computational approaches, we elucidate BPF-mediated molecular networks and reveal novel biomarkers associated with BPF exposure. Functional classes of proteins including inflammatory cytokines, ATPases, peroxidases, and aromatic l-amino decarboxylases represent novel, underexplored targets of toxicity. Most revealing of this critical review is that few studies report biological responses to BPF at levels present in aquatic environments. Recommendations for future investigations based on knowledge gaps include: (1) Mechanistic studies in the central nervous system of zebrafish to address neurotoxicity; (2) Behavioral assays in zebrafish that assess the effects of BPF on anxiolytic, social, and fear-related behaviors; (3) Studies that broaden understanding of potential endocrine disrupting effects of BPF, for example insulin signaling is predicted to be sensitive to BPF exposure; (4) Studies into metabolic disruption with a focus on glutathione and aromatic amino acids, based upon pathway analysis data; (5) Studies utilizing mixture exposures with other BPA analogs to reflect environmental conditions more accurately.
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Affiliation(s)
- Bruna Dos Santos
- Universidade Federal de Ciências da Saúde de Porto Alegre (UFCSPA), 90050-170, Porto Alegre, RS, Brazil
| | - Emma Ivantsova
- Center for Environmental and Human Toxicology, Department of Physiological Sciences, College of Veterinary Medicine, UF Genetics Institute, Interdisciplinary Program in Biomedical Sciences Neuroscience, University of Florida, Gainesville, FL, 32611, USA
| | - Andrea P Guzman
- Center for Environmental and Human Toxicology, Department of Physiological Sciences, College of Veterinary Medicine, UF Genetics Institute, Interdisciplinary Program in Biomedical Sciences Neuroscience, University of Florida, Gainesville, FL, 32611, USA
| | - Christopher J Martyniuk
- Center for Environmental and Human Toxicology, Department of Physiological Sciences, College of Veterinary Medicine, UF Genetics Institute, Interdisciplinary Program in Biomedical Sciences Neuroscience, University of Florida, Gainesville, FL, 32611, USA.
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45
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Chen H, Zhang Y, Li X, Zhang W, He H, Du B, Li T, Tang H, Liu Y, Li L, Shi M. Transcriptome Changes and Potential Immunotoxicity Analysis in RAW264.7 Macrophages Caused by Bisphenol F. Front Pharmacol 2022; 13:846562. [PMID: 35387338 PMCID: PMC8978606 DOI: 10.3389/fphar.2022.846562] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 12/31/2021] [Accepted: 02/15/2022] [Indexed: 11/17/2022] Open
Abstract
As a viable substitute for bisphenol A (BPA), BPF has been widely used in the plastic industry and daily consumer goods, resulting in its detection in humans at a comparable concentration. Evidence reveals that BPF and BPA may have similar toxic effects due to their similar structures. However, there is less information about BPF and its latent implications on the immune system, which is associated with many disorders. In this study, the in vitro toxicity of BPF on RAW264.7 macrophages was explored. The cells were treated with different concentrations of BPF (5, 10, 20, 50, 100, and 200 μM), the cell viability and apoptosis were detected, the gene expression profile was analyzed by whole-transcriptome sequencing, and the mRNA levels were detected by qRT-PCR. The results showed a high concentration of BPF could significantly reduce the survival rate of RAW264.7 macrophages. Although the medium concentration (20–50 μM) of BPF seemed to have no impact on the cell activity of macrophages, it caused the occurrence of apoptosis. The results of differential transcription showed that compared with the control group, 121 genes were upregulated and 82 genes were downregulated in the BPF group. The significantly changed gene functions were mainly concentrated in cell cycle, phagosome, lysosome, and antigen processing and presentation. These findings provide valuable information for correctly understanding the immunotoxicity risk of BPF and may help to improve the hazard identification of bisphenol compounds.
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Affiliation(s)
- Huiling Chen
- Dongguan Key Laboratory of Environmental Medicine, School of Public Health, Guangdong Medical University, Dongguan, China
| | - Yanchao Zhang
- Dongguan Key Laboratory of Environmental Medicine, School of Public Health, Guangdong Medical University, Dongguan, China
| | - Xing Li
- Dongguan Key Laboratory of Environmental Medicine, School of Public Health, Guangdong Medical University, Dongguan, China
| | - Wei Zhang
- Dongguan Key Laboratory of Environmental Medicine, School of Public Health, Guangdong Medical University, Dongguan, China
| | - Haoqi He
- Dongguan Key Laboratory of Environmental Medicine, School of Public Health, Guangdong Medical University, Dongguan, China
| | - Bohai Du
- Dongguan Key Laboratory of Environmental Medicine, School of Public Health, Guangdong Medical University, Dongguan, China
| | - Tianlan Li
- Dongguan Key Laboratory of Environmental Medicine, School of Public Health, Guangdong Medical University, Dongguan, China
| | - Huanwen Tang
- Dongguan Key Laboratory of Environmental Medicine, School of Public Health, Guangdong Medical University, Dongguan, China
| | - Yungang Liu
- Department of Toxicology, School of Public Health, Southern Medical University, Guangzhou, China
| | - Li Li
- Dongguan Key Laboratory of Environmental Medicine, School of Public Health, Guangdong Medical University, Dongguan, China
| | - Ming Shi
- Dongguan Key Laboratory of Environmental Medicine, School of Public Health, Guangdong Medical University, Dongguan, China.,Dongguan Liaobu Hospital, Dongguan, China
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Niu L, Zhang S, Wang S, An L, Manoli K, Sharma VK, Yu X, Feng M. Overlooked environmental risks deriving from aqueous transformation of bisphenol alternatives: Integration of chemical and toxicological insights. JOURNAL OF HAZARDOUS MATERIALS 2022; 427:128208. [PMID: 34999398 DOI: 10.1016/j.jhazmat.2021.128208] [Citation(s) in RCA: 9] [Impact Index Per Article: 3.0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Subscribe] [Scholar Register] [Received: 11/24/2021] [Revised: 12/29/2021] [Accepted: 12/31/2021] [Indexed: 06/14/2023]
Abstract
Owing to the widespread prevalence and ecotoxicity of bisphenol alternatives such as bisphenol S, bisphenol F, and bisphenol AF, the past decade has witnessed the publication of a remarkable number of studies related to their transformation and remediation in natural waters. However, the reactivity, removal efficiency, transformation products (TPs), and mechanisms of such emerging pollutants by different treatment processes have not been well elucidated. Particularly, the transformation-driven environmental risks have been mostly overlooked. Therefore, we present a review to address these issues from chemical and toxicological viewpoints. Four degradation systems can be largely classified as catalytic persulfate (PS) oxidation, non-catalytic oxidation, photolysis and photocatalysis, and biodegradation. It was found that bisphenol alternatives possess distinct reactivities with different oxidizing species, with the highest performance for hydroxyl radicals. All systems exhibit superior elimination efficiency for these compounds. The inadequate mineralization suggests the formation of recalcitrant TPs, from which the overall reaction pathways are proposed. The combined experimental and in silico analysis indicates that many TPs have developmental toxicity, endocrine-disrupting effects, and genotoxicity. Notably, catalytic PS systems and non-catalytic oxidation result in the formation of coupling products as well as halogenated TPs with higher acute and chronic toxicity and lower biodegradability than the parent compounds. In contrast, photolysis and photocatalysis generate hydroxylated and bond-cleavage TPs with less toxicity. Overall, this review highlights the secondary environmental risks from the transformation of bisphenol alternatives by conventional and emerging treatment processes. Finally, future perspectives are recommended to address the knowledge gaps of these contaminants in aquatic ecosystems.
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Affiliation(s)
- Lijun Niu
- College of the Environment & Ecology, Xiamen University, Xiamen 361102, China
| | - Shengqi Zhang
- College of the Environment & Ecology, Xiamen University, Xiamen 361102, China
| | - Siqin Wang
- College of the Environment & Ecology, Xiamen University, Xiamen 361102, China
| | - Lili An
- College of the Environment & Ecology, Xiamen University, Xiamen 361102, China
| | - Kyriakos Manoli
- Nireas-International Water Research Center, University of Cyprus, P.O. Box 20537, CY-1678 Nicosia, Cyprus
| | - Virender K Sharma
- Program of the Environment and Sustainability, Department of Environmental and Occupational Health, School of Public Health, Texas A&M University, College Station, TX 77843, USA
| | - Xin Yu
- College of the Environment & Ecology, Xiamen University, Xiamen 361102, China
| | - Mingbao Feng
- College of the Environment & Ecology, Xiamen University, Xiamen 361102, China.
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Wang Q, Feng Q, Hu G, Gao Z, Zhu X, Epua Epri J. Simultaneous determination of seven bisphenol analogues in surface water by solid-phase extraction and ultra-performance liquid chromatography-tandem mass spectrometry. Microchem J 2022. [DOI: 10.1016/j.microc.2021.107098] [Citation(s) in RCA: 2] [Impact Index Per Article: 0.7] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 10/19/2022]
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Jin M, Cao B, Lin C, Li J, Xu Q, Ren Q, Xu S, Tang C. Tianma Gouteng Decoction Exerts Pregnancy-Protective Effects Against Preeclampsia via Regulation of Oxidative Stress and NO Signaling. Front Pharmacol 2022; 13:849074. [PMID: 35401241 PMCID: PMC8985411 DOI: 10.3389/fphar.2022.849074] [Citation(s) in RCA: 11] [Impact Index Per Article: 3.7] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 01/05/2022] [Accepted: 03/04/2022] [Indexed: 11/30/2022] Open
Abstract
Preeclampsia (PE), a pregnancy-specific syndrome with the major molecular determinants of placenta-borne oxidative stress and consequently impaired nitric oxide (NO) generation, has been considered to be one of the leading causes of maternal morbidity as well as mortality and preterm delivery worldwide. Several medical conditions have been found to be associated with increased PE risk, however, the treatment of PE remains unclear. Here, we report that Tianma Gouteng Decoction (TGD), which is used clinically for hypertension treatment, regulates oxidative stress and NO production in human extravillous trophoblast-derived TEV-1 cells. In human preeclamptic placental explants, reactive oxygen species (ROS) levels were elevated and NO production was inhibited, while TGD treatment at different periods effectively down-regulated the H2O2-induced ROS levels and significantly up-regulated the H2O2-suppressed NO production in human TEV-1 cells. Mechanistically, TGD enhanced the activity of total nitric oxide synthase (TNOS), which catalyze L-arginine oxidation into NO, and simultaneously, TGD promoted the expression of neuronal nitric oxide synthase (nNOS) and endothelial nitric oxide synthase (eNOS), two isoforms of nitric oxide synthetases (NOS) in human placenta, resulting in the increased NO generation. More importantly, TGD administration not only increased the weight gain during pregnancy and revealed a hypotensive effect, but also improved the placental weight gain and attenuated fetal growth restriction in an NG-nitro-L-arginine methyl ester (L-NAME)-induced mouse PE-like model. Our results thereby provide new insights into the role of TGD as a potentially novel treatment for PE.
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Affiliation(s)
- Meiyuan Jin
- National Clinical Research Center for Child Health of the Children's Hospital, Zhejiang University School of Medicine, Hangzhou, China
- Department of Obstetrics, Tongde Hospital of Zhejiang Province, Hangzhou, China
| | - Bin Cao
- Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou, China
| | - Chao Lin
- Department of Neurosurgery, The Children's Hospital, Zhejiang University School of Medicine, Hangzhou, China
| | - Jiayong Li
- Department of Ophthalmology, Hangzhou Traditional Chinese Medicine Hospital Affiliated to Zhejiang Chinese Medical University, Hangzhou, China
| | - Qiang Xu
- National Clinical Research Center for Child Health of the Children's Hospital, Zhejiang University School of Medicine, Hangzhou, China
| | - Qianlei Ren
- National Clinical Research Center for Child Health of the Children's Hospital, Zhejiang University School of Medicine, Hangzhou, China
| | - Shouying Xu
- National Clinical Research Center for Child Health of the Children's Hospital, Zhejiang University School of Medicine, Hangzhou, China
| | - Chao Tang
- National Clinical Research Center for Child Health of the Children's Hospital, Zhejiang University School of Medicine, Hangzhou, China
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Castro G, Fourie AJ, Marlin D, Venkatraman V, González SV, Asimakopoulos AG. Occurrence of bisphenols and benzophenone UV filters in wild brown mussels (Perna perna) from Algoa Bay in South Africa. THE SCIENCE OF THE TOTAL ENVIRONMENT 2022; 813:152571. [PMID: 34954183 DOI: 10.1016/j.scitotenv.2021.152571] [Citation(s) in RCA: 1] [Impact Index Per Article: 0.3] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Subscribe] [Scholar Register] [Received: 09/25/2021] [Revised: 12/16/2021] [Accepted: 12/16/2021] [Indexed: 06/14/2023]
Abstract
Bisphenols and benzophenone UV filters are contaminants present in a wide variety of plastic materials and consumer products. The scientific attention towards these contaminants has increased in recent years due to their presence in microplastics, their ubiquitous occurrence in the environment, and their known endocrine disrupting health effects. In this study, the occurrence of nine bisphenol and five benzophenone UV-filter analogues was assessed in wild brown mussels (Perna perna) collected from different sampling sites along the coast of Algoa Bay, South Africa. Eleven out of fourteen target analytes were detected, and bisphenol AP (BPAP) was detected for the first time in mussels, presenting the highest median concentration of 150 ng g-1 dry weight (d.w.) and a detection frequency of 91%. Regarding benzophenone UV-filters, median concentrations of the analogues (across all sites) ranged from 2.01 to 10.6 ng g-1 d.w., with benzophenone-1 (BzP-1) and benzophenone-3 (BzP-3) presenting the highest concentrations. Human exposure was assessed by estimating daily intakes (EDI) of the target analytes through mussel consumption. To our knowledge, this is the first study from the African continent on the occurrence of bisphenols and benzophenone UV-filters in a large population (n=138) of wild brown mussels.
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Affiliation(s)
- Gabriela Castro
- Department of Chemistry, Norwegian University of Science and Technology (NTNU), NO-7491 Trondheim, Norway
| | - Amarein J Fourie
- Sustainable Seas Trust, 222 Main Road, Walmer, Port Elizabeth 6070, South Africa
| | - Danica Marlin
- Sustainable Seas Trust, 222 Main Road, Walmer, Port Elizabeth 6070, South Africa
| | - Vishwesh Venkatraman
- Department of Chemistry, Norwegian University of Science and Technology (NTNU), NO-7491 Trondheim, Norway
| | - Susana V González
- Department of Chemistry, Norwegian University of Science and Technology (NTNU), NO-7491 Trondheim, Norway
| | - Alexandros G Asimakopoulos
- Department of Chemistry, Norwegian University of Science and Technology (NTNU), NO-7491 Trondheim, Norway.
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50
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Edaes FS, de Souza CB. BPS and BPF are as Carcinogenic as BPA and are Not Viable Alternatives for its Replacement. Endocr Metab Immune Disord Drug Targets 2022; 22:927-934. [PMID: 35297356 DOI: 10.2174/1871530322666220316141032] [Citation(s) in RCA: 10] [Impact Index Per Article: 3.3] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Submit a Manuscript] [Subscribe] [Scholar Register] [Received: 09/03/2021] [Revised: 11/08/2021] [Accepted: 12/28/2021] [Indexed: 11/22/2022]
Abstract
BACKGROUND Plastic polymers are omnipresent, and life without them is virtually impossible. Despite the advantages provided by the material, conventional plastic also has harmful effects on the environment and human health. Plastics release microplastics and compounds, such as BPA, which is a xenoestrogen and once absorbed by the body, have an affinity for estrogen receptors α and β, acting as an agonist on human cells, being an endocrine disrupter able to cause various diseases and acting as a potential neoplastic inducer. BPS and BPF are BPA's analogs, a proposed solution to solve its harmful effects incorporated into the market. The analogs can be found in daily use products and are used in several industrial applications. OBJECTIVES In the present work, the researchers aimed to develop a revisional study of BPA's harmful effects on human health, focusing on its carcinogenic potential, discussing its mechanisms of action, as well as its analogs effects, and identifying if they are a viable alternative to BPA's substitution in plastic polymers' production. METHODS In this review, articles published in the last 15 years related to the different aspects of conventional plastics and BPA were analyzed and revised with precision. The subjects ranged from conventional plastics and the problems related to their large-scale production, BPA, its negative aspects, and the feasibility of using its analogs (BPS and BPF) to replace the compound. The articles were extensively reviewed and concisely discussed. RESULTS This study demonstrated that BPA has a high carcinogenic potential, with known mechanisms to trigger breast, ovarian, prostate, cervical, and lung cancers, thus elucidating that its analogs are also xenoestrogens, that they can exert similar effects to BPA and, therefore, cannot be considered viable alternatives for its replacement. Conclusion This study suggests that new research should be carried out to develop such alternatives, allowing the substitution of plastic materials containing BPA in their composition, such as developing economically viable and sustainable biodegradable bioplastics for socio-environmental well-being.
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Affiliation(s)
- Felipe Sanches Edaes
- Academic Center for Studies and Research in Biotechnology and Molecular Biology (NAPBBM), Lusíada University Center (UNILUS), Santos, Brazil
| | - Cleide Barbieri de Souza
- Academic Center for Studies and Research in Biotechnology and Molecular Biology (NAPBBM), Lusíada University Center (UNILUS), Santos
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