Novel diet-related mouse model of colon cancer parallels human colon cancer

Table 1 AIN-93G diet composition

Ingredients	Percentage
Corn starch	39.75%
Casein vitamin free	20%
Maltodextrin	13.20%
Sucrose	10%
Soybean oil	7%
Powdered cellulose	5%
AIN 93G mineral mix	3.50%
AIN 93 vitamin mix	1%
L-cystine	0.30%
Choline bitartrate	0.25%
t-butylhydroquinone	0.0014%

Table 2 Mice fed diet + deoxycholic acid

Mouse weights (g)	Locations of tumors		Hyper-plastic polyp	Characteristics of polyps low and high grade dysplasia including those from which cancers arose				Stages of cancers found
	Distal tumor	Proximal tumor		Tubular adenoma	Sessile serrated adenoma	Ulcerated adenoma	Adenoma with HGD	Intra-mucosal AC	Stage T1 AC	Stage T2 AC
18.7	3			3
24		3		2			1	1
25	2			2
25.8	1				1	1			1
25.9		3		3		2	1
26.1		1		1		1				1
26.1	3			3		2			2
27.3		2		2		1		1
27.4		5		5		3		3
28.8		2		2
35.4	None	None
35.7	2	3	2	3
38.9	2	2		2	2
40		3		2						1
41.1		4			3	3		1	2
43		2			2	1
43.1		1		1		1
45.2		1		1		1	1
45.2		2		2				1
49.2	None	None
53.7		7		3	4	1			4
78.6		3			3
Totals	13	44	2	37	15	17	3	7	9	2

AC: Adenocarcinoma; HGD: Highgrade dysplasia.

Table 3 Comparison of diet alone to diet + deoxycholic acid on colonic tumor and cancer development n (%)

Diet	Diet (mo)	Mice	Mice with tumors(adenomas + cancers)	Mice with cancer	Tumors(tumor burden1)	Cancers(cancer burden2)
Diet alone	10	22	0	0	0 (0)	0 (0)
Diet + DOC	10	22	20 (91%)	10 (45%)	57 (2.6)	18 (0.82)

¹Tumor burden is the ratio of the number of tumors observed to the number of mice;

²Cancer burden is the ratio of the number of cancers observed to the number of mice. DOC: Deoxycholic acid.