Transmembrane protein 176B promotes epithelial-mesenchymal transition in colorectal cancer through inflammasome inhibition

Table 1 Antibody information in western blot

Antibody	Company	Cat#	Dilution ratio
NLRP3 antibody	Affinity	DF7438	1:1000
ASC antibody	Santa Curz Biotechnology	sc-22514-R	1:1000
Caspase1 antibody	Affinity	AF5418	1:1000
IL18 antibody	Affinity	DF6252	1:1000
TMEM176B antibody	Bioss	bs-11876R	1:1000
p-p65 antibody	Affinity	AF2006	1:1000
p65 antibody	Affinity	AF5006	1:1000
N-cadherin antibody	Affinity	AF4039	1:1000
E-cadherin antibody	Proteintech	20874-1-AP	1:1000
Vimentin antibody	Abcam	ab20346	1:1000
MMP-9 antibody	Proteintech	10375-2-AP	1:1000
TGF-β antibody	Affinity	AF1027	1:1000
Anti-rabbit IgG, HRP-linked antibody	CST	7074	1:6000
Anti-mouse IgG, HRP-linked antibody	CST	7076	1:6000
GAPDH antibody	Proteintech	10494-1-AP	1:10000

HRP: Horseradish peroxidase.

Table 2 Primers of genes in quantitative reverse transcription PCR

Gene	Forward primer (5’-3’)	Reverse primer (5’-3’)
Mouse Teme176b	AGTCCGCTCACATCAGCATC	ACTTAGTCCCAGGGAAGCCA
Mouse NLRP3	CTACGGCCGTCTACGTCTTC	GGCCAAAGAGGAATCGGACA
Mouse GAPDH	GGGTCCCAGCTTAGGTTCAT	CTCGTGGTTCACACCCATCA