LncRNA PCAT6 promotes progression and metastasis of colonic neuroendocrine carcinoma via MAPK pathway

Table 1 Relationship between prostate cancer associated transcript 6 expression and clinicopathologic variables of colonic neuroendocrine carcinomas

Clinical features	n	PCAT6 expression		P value
		High (16)	Low (14)
Age				0.4642
≤ 65	15	7	8
> 65	15	9	6
Gender				0.7321
Male	16	9	7
Female	14	7	7
Grade				0.5257
I-II	23	13	10
III	7	3	4
TNM
I-II	13	4	9	*0.0303
III-IV	17	12	5
Invasion depth				0.8808
T1+T2	3	2	1
T3	19	10	9
T4	8	4	4
Lymphatic metastasis				0.0281
Absent	15	5	10
Present	15	11	4
Distant metastasis
Absent	17	6	11	0.0235
Present	13	10	3
Death
Absent	14	4	10	0.0110
Present	16	12	4

Table 2 Sequences of primers used for reverse transcription-quantitative polymerase chain reaction

Gene (human)	Sequence (5’-3’)
PCAT6 forward	TCCTCATTCGGTCCATCCAACTCC
PCAT6 reverse	GAAGCACGAGCAAGGCAGAGAC
CgA forward	CTCCAGGTCCGAGGCTAC
CgA reverse	GACAGGCTCTCCAGCTCC
E-cadherin forward	GGAACTATGAAAAGTGGGCTTG
E-cadherin reverse	AAATTGCCAGGCTCAATGAC
N-cadherin forward	GGTGGAGGAGAAGAAGACCAG
N-cadherin reverse	GGCATCAGGCTCCACAGT
Snail forward	CTTCCAGCAGCCCTACGAC
Snail reverse	CGGTGGGGTTGAGGATCT
Vimentin forward	GACGCCATCAACACCGAGTT
Vimentin reverse	CTTTGTCGTTGGTTAGCTGGT
miR-4731-5p forward	TGCTGGGGGCCACAT
miR-4731-5p reverse	CTCTACAGCTATATTGCCAGCCAC
miR-1306-5p forward	CCACCTCCCCTGCAAA
miR-1306-5p reverse	TCCTCCTCTCCTTCCTTCTC
miR-330-5p forward	TCTCTGGGCCTGTGTCTTAG
miR-330-5p reverse	CAGTGCGTGTCGTGGAGT
miR-326 forward	ACTGTCCTTCCCTCTGGGC
miR-326 reverse	AATGGTTGTTCTCCACTCTCTCTC
U6 forward	CTCGCTTCGGCAGCACA
U6 reverse	AACGCTTCACGAATTTGCGT
GAPDH forward	CATGAGAAGTATGACAACAGCCT
GAPDH reverse	AGTCCTTCCACGATACCAAAGT