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©The Author(s) 2024.
World J Gastrointest Oncol. Mar 15, 2024; 16(3): 979-990
Published online Mar 15, 2024. doi: 10.4251/wjgo.v16.i3.979
Published online Mar 15, 2024. doi: 10.4251/wjgo.v16.i3.979
Primer name | Primer information | Base sequences (5’-3’) | Tm value | Product length | |
M-GAPDH | NM_008084.3 | Sense | TGAAGGGTGGAGCCAAAAG | 58.3 | 227 |
Anti-sense | AGTCTTCTGGGTGGCAGTGAT | 58.4 | |||
M-wnt1 | NM_021279 | Sense | GCAGCCTCTTCTCACTGCAG | 61.0 | 148 |
Anti-sense | CCCAGGCTGGCTCTAATAAGT | 59.2 | |||
M-β-catenin | NM_007614 | Sense | TCCGAGGACTCAATACCATTCC | 60.6 | 250 |
Anti-sense | CGCTTCTTGTAATCCTGTGGC | 60.0 | |||
M-cyclinD1 | NM_007631.2 | Sense | GGATGAGAACAAGCAGACCATC | 58.6 | 186 |
Anti-sense | AGAAAGTGCGTTGTGCGGTA | 59.2 | |||
M-EGF | NM_010113 | Sense | TTATGACCCTGTGGAAAGCAAG | 58.6 | 119 |
Anti-sense | CAAGCGTATCTACTCCTTCTGTGAT | 60.2 | |||
M-c-myc | NM_001177352.1 | Sense | GGACTGTATGTGGAGCGGTTT | 59.1 | 208 |
Anti-sense | GTTGAGCGGGTAGGGAAAGA | 59.3 |
Norm | Control group | MNU group | H. pylori + MNU group | Moluodan group | F value | P value |
Wnt1 | 1.10 ± 0.12 | 3.69 ± 0.16a | 4.72 ± 0.06a,b | 2.99 ± 0.19a,b,c | 347.51 | < 0.001 |
β-catenin | 1.03 ± 0.03 | 2.74 ± 0.11a | 3.73 ± 0.12a,b | 1.70 ± 0.06a,b,c | 523.67 | < 0.001 |
CyclinD1 | 1.06 ± 0.17 | 3.22 ± 0.07a | 3.84 ± 0.16a,b | 2.17 ± 0.11a,b,c | 248.70 | < 0.001 |
EGF | 1.08 ± 0.07 | 2.96 ± 0.14a | 4.32 ± 0.24a,b | 2.17 ± 0.11a,b,c | 230.00 | < 0.001 |
c-Myc | 1.10 ± 0.14 | 2.80 ± 0.14a | 4.40 ± 0.08a,b | 1.97 ± 0.11a,b,c | 411.91 | < 0.001 |
Norm | Control group | MNU group | H. pylori + MNU group | Moluodan group | F value | P value |
Wnt1 | 0.05 ± 0.01 | 0.29 ± 0.02a | 0.58 ± 0.02a,b | 0.12 ± 0.02a,b,c | 479.39 | < 0.001 |
β-catenin | 0.06 ± 0.04 | 0.26 ± 0.08a | 0.43 ± 0.06a,b | 0.16 ± 0.06a,b,c | 18.80 | < 0.001 |
cyclinD1 | 0.09 ± 0.04 | 0.31 ± 0.12a | 0.60 ± 0.10a,b | 0.15 ± 0.07a,b,c | 11.59 | 0.003 |
EGF | 0.06 ± 0.03 | 0.17 ± 0.02a | 0.39 ± 0.08a,b | 0.09 ± 0.02a,b,c | 35.26 | < 0.001 |
c-Myc | 0.05 ± 0.01 | 0.23 ± 0.06a | 0.54 ± 0.06a,b | 0.13 ± 0.02a,b,c | 64.19 | < 0.001 |
- Citation: Wang YM, Luo ZW, Shu YL, Zhou X, Wang LQ, Liang CH, Wu CQ, Li CP. Effects of Helicobacter pylori and Moluodan on the Wnt/β-catenin signaling pathway in mice with precancerous gastric cancer lesions. World J Gastrointest Oncol 2024; 16(3): 979-990
- URL: https://www.wjgnet.com/1948-5204/full/v16/i3/979.htm
- DOI: https://dx.doi.org/10.4251/wjgo.v16.i3.979