Basic Study
Copyright ©The Author(s) 2024.
World J Gastrointest Oncol. Mar 15, 2024; 16(3): 979-990
Published online Mar 15, 2024. doi: 10.4251/wjgo.v16.i3.979
Table 1 Reverse transcription primers of each gene
Primer name
Primer information
Base sequences (5’-3’)
Tm value
Product length
M-GAPDHNM_008084.3SenseTGAAGGGTGGAGCCAAAAG58.3227
Anti-senseAGTCTTCTGGGTGGCAGTGAT58.4
M-wnt1NM_021279SenseGCAGCCTCTTCTCACTGCAG61.0148
Anti-senseCCCAGGCTGGCTCTAATAAGT59.2
M-β-cateninNM_007614SenseTCCGAGGACTCAATACCATTCC60.6250
Anti-senseCGCTTCTTGTAATCCTGTGGC60.0
M-cyclinD1NM_007631.2SenseGGATGAGAACAAGCAGACCATC58.6186
Anti-senseAGAAAGTGCGTTGTGCGGTA59.2
M-EGFNM_010113SenseTTATGACCCTGTGGAAAGCAAG58.6119
Anti-senseCAAGCGTATCTACTCCTTCTGTGAT60.2
M-c-mycNM_001177352.1SenseGGACTGTATGTGGAGCGGTTT59.1208
Anti-senseGTTGAGCGGGTAGGGAAAGA59.3
Table 2 Comparison of relative expression of Wnt1, β-catenin, cyclinD1, epidermal growth factor, and c-Myc m-RNA in each group
Norm
Control group
MNU group
H. pylori + MNU group
Moluodan group
F value
P value
Wnt11.10 ± 0.123.69 ± 0.16a4.72 ± 0.06a,b2.99 ± 0.19a,b,c347.51< 0.001
β-catenin1.03 ± 0.032.74 ± 0.11a3.73 ± 0.12a,b1.70 ± 0.06a,b,c523.67< 0.001
CyclinD11.06 ± 0.173.22 ± 0.07a3.84 ± 0.16a,b2.17 ± 0.11a,b,c248.70< 0.001
EGF1.08 ± 0.072.96 ± 0.14a4.32 ± 0.24a,b2.17 ± 0.11a,b,c230.00< 0.001
c-Myc1.10 ± 0.142.80 ± 0.14a4.40 ± 0.08a,b1.97 ± 0.11a,b,c411.91< 0.001
Table 3 Comparison of Wnt1, β-catenin, cyclinD1, epidermal growth factor, and c-Myc protein expression in each group
Norm
Control group
MNU group
H. pylori + MNU group
Moluodan group
F value
P value
Wnt10.05 ± 0.010.29 ± 0.02a0.58 ± 0.02a,b0.12 ± 0.02a,b,c479.39< 0.001
β-catenin0.06 ± 0.040.26 ± 0.08a0.43 ± 0.06a,b0.16 ± 0.06a,b,c18.80< 0.001
cyclinD10.09 ± 0.040.31 ± 0.12a0.60 ± 0.10a,b0.15 ± 0.07a,b,c11.590.003
EGF0.06 ± 0.030.17 ± 0.02a0.39 ± 0.08a,b0.09 ± 0.02a,b,c35.26< 0.001
c-Myc0.05 ± 0.010.23 ± 0.06a0.54 ± 0.06a,b0.13 ± 0.02a,b,c64.19< 0.001