Expression of nucleus accumbens-1 in colon cancer negatively modulates antitumor immunity

doi:10.4251/wjgo.v14.i12.2329

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World Journal of Gastrointestinal Oncology

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1948-5204

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Gastrointest Oncol. Dec 15, 2022; 14(12): 2329-2339
Published online Dec 15, 2022. doi: 10.4251/wjgo.v14.i12.2329

Table 1 siRNA sequences used in gene knockdown

Gene name		Primer sequence (5’→3’)
Negative control	Sense	UUCUCCGAACGUGUCACGUTT
	Anti-sence	ACGUGACACGUUCGGAGAATT
siNAC-1	Sense	GGACAUGAUGAGCAUGGAATT
	Anti-sence	UUCCAUGCUCAUCAUGUCCTT
siCD274	Sense	GAGGAAGACCUGAAGGUUCAGCAUA
	Anti-sence	UAUGCUGAACCUUCAGGUCUUCCUC

NAC-1: Nucleus accumbens-1.

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Table 2 Human primers used in quantitative real-time-polymerase chain reaction

Gene name		Primer sequence (5’→3’)
NAC-1	Forward	TTCTTTGACCGGAACACGCT
	Reverse	TGGCATTCATCTCGCTCTCC
PD-L1	Forward	CCCTAATTTGAGGGTCAGTTCCT
	Reverse	CTCAGTCATGCAGAAAACAAATTGA
GAPDH	Forward	GACAGTCAGCCGCATCTTCT
	Reverse	TTAAAAGCAGCCCTGGTGAC

NAC-1: Nucleus accumbens-1; PD-L1: Programmed death receptor-1 ligand.

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Citation: Shen ZH, Luo WW, Ren XC, Wang XY, Yang JM. Expression of nucleus accumbens-1 in colon cancer negatively modulates antitumor immunity. World J Gastrointest Oncol 2022; 14(12): 2329-2339
URL: https://www.wjgnet.com/1948-5204/full/v14/i12/2329.htm
DOI: https://dx.doi.org/10.4251/wjgo.v14.i12.2329