Long-term follow-up of HER2 overexpression in patients with rectal cancer after preoperative radiotherapy: A prospective cohort study

Abstract

BACKGROUND

The role of HER2 overexpression in rectal cancer is controversial.

AIM

To assess the role of HER2 overexpression in the long-term prognosis of rectal cancer.

METHODS

Data from patients with locally advanced rectal cancer who underwent total mesorectal excision after short-course radiotherapy at Beijing Cancer Hospital between May 2002 and October 2005 were collected. A total of 151 tissue samples of rectal cancer were obtained using rigid proctoscopy before neoadjuvant radiotherapy, followed by immunohistochemistry and fluorescence in situ hybridisation to determine the patients’ HER2 expression status. Univariate and multivariate analyses of the associations between the clinicopathological factors and HER2 status were performed. Survival was estimated and compared using the Kaplan-Meier method based on HER2 expression status, and the differences between groups were verified using the log-rank test.

RESULTS

A total of 151 patients were enrolled in this study. A total of 27 (17.9%) patients were ultimately confirmed to be HER2-positive. The follow-up duration ranged from 9 mo to 210 mo, with a median of 134 mo. Distant metastasis and local recurrence occurred in 60 (39.7%) and 24 (15.9%) patients, respectively. HER2 positivity was significantly associated with the pre-treatment lymph node stage (pre-N) (P = 0.040), while there were no differences between HER2 status and age, sex, preoperative CEA levels (pre-CEA), T stage, and lympho-vascular invasion. In terms of prognosis, HER2 overexpression was correlated with distant metastasis (P = 0.002) rather than local recurrence (P > 0.05). The multivariate analysis demonstrated that elevated pre-CEA [P = 0.002, odds ratio (OR) = 3.277, 97.5% confidence interval (CI): 1.543-7.163], post N(+) (P = 0.022, OR = 2.437, 97.5%CI: 1.143-5.308) and HER2(+) (P = 0.003, OR = 4.222, 97.5%CI: 1.667-11.409) were risk factors for distant metastasis. The survival analysis showed that there were significant differences between rectal cancer patients in terms of disease-free survival (DFS) [hazard ratio: 1.69 (95%CI: 0.91-3.14); P = 0.048] and overall survival (OS) [1.95 (1.05-3.63); P = 0.0077].

CONCLUSION

HER2 overexpression is a potential biomarker for predicting lymph node metastasis and distant metastasis, which are associated with worse long-term DFS and OS in rectal cancer patients with locally advanced disease.

Key Words: HER2; Rectal cancer; Distant metastasis; Local recurrence; Survival

Core Tip: Long-term follow-up of rectal cancer patients treated with neoadjuvant radiotherapy demonstrated that pre-treatment HER2 overexpression was significantly correlated with lymph node metastasis and long-term distant metastasis. Furthermore, HER2 overexpression, elevated CEA and lymph node positivity were independent risk factors, predictive for poorer survival.

INTRODUCTION

Colorectal cancer (CRC) is a great challenge for people worldwide and is the 3^rd most commonly diagnosed cancer and the 2^nd leading cause of cancer-related deaths. In China, the incidence and death rates of CRC are the 3^rd and 5^th highest, respectively[1,2]. In 2020, there were about 43340 new cases of rectal cancer in the United States each year, while this number was 376000 in China[2,3]. Due to its insidious onset, most patients are diagnosed with locally advanced disease, and approximately 20% of CRC patients have distant metastasis at the time of diagnosis[4]. A series of studies, including the SWEDISH RECTAL CANCER TRIAL in the 1990s[5], the CAO/ARO/AIO-94 trial performed in Germany in the early 21^st century[6], and the subsequent EORTC22921 study[7], have shown the benefits of tumour regression, sphincter preservation, and decreased local recurrence, establishing the status of neoadjuvant radiotherapy. In recent years, the treatment mode of rectal cancer guided by TNM staging has been evolving; notably, the long-term survival rates have appeared to remain stable[8-10]. In addition, even patients with the same TNM stage may have different prognoses, prompting us to search for new therapeutic biomarkers to improve patient outcomes. In recent years, the molecular mechanisms of CRC have been further studied, and biological markers such as RAS and BRAF mutations have been identified as prognostic targets. In contrast, HER2, a member of the epidermal growth factor receptor family of receptor tyrosine kinases, is highly expressed in a variety of tumour cells. HER2 is currently considered a potential target for CRC therapy, as reported by previous findings[11]. Although there have been several studies on the role of HER2 in CRC, the results are controversial and lack long-term outcomes. The present study aimed to report the long-term outcomes of patients with HER2 overexpression in locally advanced rectal cancer with preoperative radiotherapy.

MATERIALS AND METHODS

Data from patients with locally advanced rectal cancer who underwent total mesorectal excision (TME) after short-course radiotherapy at the Beijing Cancer Hospital between May 2002 and October 2005 were collected. All patients underwent preoperative staging using magnetic resonance imaging, computed tomography, endorectal ultrasonography, and/or endoscopy. Each patient enrolled in our study satisfied the following criteria: (1) Age > 18 years; (2) Primary rectal adenocarcinoma below the peritoneal folds; (3) Clinically staged as cT3-4 and/or N+ rectal tumours, with a tumour that could be resected radically; and (4) Willingness to participate in long-term follow-up. Patients were excluded if they: (1) Had synchronous tumours or a history of other malignant tumours within the previous 5 years; (2) Had a previous history of cytotoxic chemotherapy, previous pelvic radiation therapy, or a known hypersensitivity to any drug included in the treatment protocol; (3) Had a diagnosis of hereditary nonpolyposis colorectal carcinoma; (4) Were being treated with other experimental drugs or had previously participated in a clinical trial of other experimental agents for rectal carcinoma; and (5) Had clinical evidence of distant metastasis. There were no restrictions based on sex, race, or disability.

All patients received 30 Gy/10 F/2 W neoadjuvant radiotherapy (SIMENS PRIMUS 2916 Linear Accelerator), and radical surgeries (LAR or APR ) were performed 2 wk after the end of radiotherapy, according to the TME principle. All patients received adjuvant chemotherapy (standard regimen, 5-FU or capecitabine) within 6 mo after surgery, according to the pathologic stage. Patient epidemiological information and primary tumour features, including distance from the anal verge and TNM stage before and after neoadjuvant radiotherapy, were collected prospectively. Regular follow-up visits were performed every 3 mo for the first 2 years, and then every 6 mo for a total of 5 years. After 5 years, follow-up was performed once per year. Follow-up examinations included blood tests for CEA and CA199, thoracic and abdominal/pelvic computed tomography or magnetic resonance imaging, and enteroscopy for timely detection of recurrence or metastasis. The study was approved by the medical ethics committee of the Peking University Cancer Hospital, and the requirement for informed consent was waived.

Immunohistochemical evaluation

All patients underwent rigid proctoscopy before neoadjuvant therapy, and a sufficient amount of tumour tissue was obtained. HER2 expression was evaluated using immunohistochemistry. Immunostained samples were examined and scored independently and in a blinded manner by two experienced pathologists. The scoring criteria were as follows[12]: 0 (no staining), 1+ (1%-25% positive cells), 2+ (26%-75% positive cells), or 3+ (76%-100% positive cells). A score of 0 or 1+ was considered negative (low expression) and a score of 3+ was considered positive (overexpression). In samples when the score was 2+ (moderate expression), fluorescence in situ hybridization (FISH) was performed to confirm the HER2 expression.

Statistical analysis

Data were analysed using R4.1.0 software. The ‘survival’ and ‘survminer’ package were used for statistical analysis, and the ‘ggplot2’ package was used for plotting. Categorical variables were assessed using the chi-square (2 × 2) or Fisher's exact test (2 × C) when applicable. Multivariate analysis was performed using a binary logistic regression model (forward: LR). Survival analysis was performed using the Kaplan-Meier method, and the differences between groups were verified using the log-rank test. P values < 0.05 were considered significant statistically.

RESULTS

In our previous reports, a total of 142 rectal cancer patients with locally advanced diseases were enrolled, followed by nine more patients meeting the inclusion criteria; finally, 151 patients were included in the final analysis (Figure 1). The mean age was 55.85 ± 13.26 years, with 93 (61.6%) men and 58 (38.4%) women. The patient characteristics are listed in Table 1.

Figure 1 Flowchart of cohorts and the distribution of HER2 expression. FISH: Fluorescence in situ hybridization.

Table 1 Clinicopathological characteristics of the enrolled cohort (n = 151).

Characteristic	Result
Age diagnosis (yr)	55.85 ± 13.26
Gender (%)
Male	93 (61.6)
Female	58 (38.4)
Distance to anal verge (cm) (%)	4.91 ± 2.04
> 6	45 (29.8)
≤ 6	106 (70.2)
Pre-CEA (%)
Normal range	98 (64.9)
Elevated	53 (35.1)
Pre-T (%)
T1	2 (1.3)
T2	10 (6.6)
T3	133 (88.1)
T4	6 (4.0)
Pre-N (%)
N0	38 (25.2)
N+	113 (74.8)
Post-T (%)
pT0	7 (4.6)
pT1	6 (4.0)
pT2	48 (31.8)
pT3	84 (55.6)
pT4	4 (4.0)
Post-N (%)
pN0	89 (58.9)
pN+	62 (41.1)
Tumor regression grade (%)
Grade 0	9 (6.3)
Grade 1	34 (23.9)
Grade 2	42 (29.6)
Grade 3	57 (40.1)
HER2 (%)
-	124 (82.1)
+	27 (17.9)
LVI (%)
+	20 (13.2)
-	131 (86.8)
Median follow-up period	134
Distant metastasis, number (%)
Yes	60 (39.7)
No	91 (60.3)
Local recurrence, number (%)
Yes	24 (15.9)
No	127 (84.1)

Pre-CEA: Preoperative CEA; LVI: Lympho-vascular invasion.

The immunohistochemical results showed that HER2 over-expression was detected in 16.6% (25/151) of the tissue samples, with low expression (0-1+) in 73.51% (111/151) and moderate expression in 9.93% (15/151) of samples. In samples (n = 2) that scored 2+, we confirmed the positive expression of HER2 using FISH. A total of 27/151 (17.9%) samples were ultimately confirmed to have HER2 positivity. The median follow-up period for all patients was 134 mo. Distant metastasis and local recurrence occurred in 60 (39.7%) and 24 (15.9%) patients, respectively.

Correlation between HER2 overexpression and clinicopathological parameters

There were no significant differences between HER2 status and age, sex, preoperative CEA levels (pre-CEA), T stage, lymph-vascular invasion (LVI), and local recurrence. HER2 positivity was associated with pre-treatment N(+) stage (pre-N; P = 0.040) and distant metastasis (P = 0.002). Distant metastasis occurred in 66.7% (18/27) of HER2-positive patients compared to in 33.9% (42/124) of HER2-negative patients (Table 2).

Table 2 Correlations among HER2 expression and the clinicopathological parameters.

Variable	HER2- (%)	HER2+ (%)	χ2	P value
Age (yr)			0.558	0.455
< 60	74 (59.7)	14 (51.9)
≥ 60	50 (40.3)	13 (48.1)
Sex			2.166	0.141
Male	73 (58.9)	20 (74.1)
Female	51 (41.1)	7 (25.9)
Pre-CEA			0.045	0.832
Normal range	80 (64.5)	18 (66.7)
Elevated	44 (35.5)	9 (33.3)
Distance to anal verge (cm)			0.236	0.627
> 6	38 (30.6)	7 (25.9)
≤ 6	86 (69.4)	20 (74.1)
Pre-T				0.6941
T1-2	11	1
T3-4	113	26
Pre-N			4.235	0.040
N0	27 (21.8)	11 (40.7)
N+	97 (78.2)	16 (59.3)
Post-T			1.583	0.208
T1-2	53	8
T3-4	71	19
Post-N			1.582	0.208
N0	76 (61.3)	13 (48.1)
N+	48 (38.7)	14 (51.9)
LVI
+	17 (13.7)	3 (11.1)	0.002	0.9622
-	107 (86.3)	24 (88.9)
Distant metastasis			9.959	0.002
+	42 (33.9)	18 (66.7)
-	82 (66.1)	9 (33.3)
Local recurrence			1.083	0.2982
+	22 (17.7)	2 (7.4)
-	102 (82.3)	25 (92.6)

¹Fisher’s exact test.

²Logistic regression model.

LVI: Lympho-vascular invasion.

Correlation between distant metastasis and clinicopathological parameters

Univariate analysis showed that pre-CEA, pre-treatment T stage (pre-T), post-treatment N status (Post-N), and HER2 status were correlated with distant metastasis (Table 3). These variables were further included in the binary logistic regression analysis, and the P value (0.052) of post-T was close to 0.05; therefore, it was also included in the multivariate analysis. The final analysis showed that elevated pre-CEA [P = 0.002, odds ratio (OR) = 3.277, 97.5% confidence interval (CI): 1.543-7.163], post-N(+) (P = 0.022, OR = 2.437, 97.5%CI: 1.143-5.308), and HER2(+) (P = 0.003, OR = 4.222, 97.5%CI: 1.667-11.409) were risk factors for distant metastasis, as demonstrated in Table 4.

Table 3 Correlations among distant metastasis and the clinicopathological parameters.

Variable	Distant metastasis		χ2	P value
	- (%)	+ (%)
Age (yr)			0.121	0.728
< 60	52 (59.1)	36 (40.9)
≥ 60	39 (61.9)	24 (38.1)
Sex			0.446	0.504
Male	58 (62.4)	35 (37.6)
Female	33 (56.9)	25 (43.1)
Pre-CEA			9.704	0.002
Normal range	68 (69.4)	30 (30.6)
Elevated	23 (43.4)	30 (56.6)
Distance to anal verge (cm)			0.103	0.749
> 6	28 (62.2)	17 (37.8)
≤ 6	63 (59.4)	43 (40.6)
Pre-T				0.2211
T1-2	5	7
T3-4	86	53
Pre-N			0.001	1.000
N0	23 (60.5)	15 (39.5)
N+	68 (60.2)	45 (39.8)
Post-T			3.782	0.052
T1-2	43	18
T3-4	48	42
Post-N			7.995	0.005
N0	62 (69.7)	27 (30.3)
N+	29 (46.8)	33 (53.2)
LVI			1.014	0.314
+	10 (50.0)	10 (50.0)
-	81 (61.8)	50 (38.2)
HER2, number			9.959	0.002
-	82 (66.1)	42 (33.9)
+	9 (33.3)	18 (66.7)

¹Fisher’s exact test.

Table 4 Multivariate logistic regression analysis results.

Variable	Odds ratio	97.5%CI	P value
CEA level before radiotherapy
Normal range	1
Elevated	3.277	1.543-7.163	0.002
Post-treatment N
N0	1
N+	2.437	1.143-5.308	0.022
HER2
-	1
+	4.222	1.667-11.409	0.003

CI: Confidence interval.

Survival analysis

The follow-up time ranged from 9 mo to 210 mo, with a median follow-up of 134 mo. During the follow-up period, 18 (66.7%) of the 27 HER2-positive patients experienced distant metastasis or recurrence, and 2 of these patients suffered from both metastasis and recurrence; thus, in terms of disease progression, there were a total of 18 (66.7%) distant metastases and 2 (7.4%) local recurrence events. The median disease-free survival (DFS) of HER2-positive patients was 43 mo. For HER2-negative patients, 57 (46.0%) out of 124 developed distant metastasis or recurrence. Death occurred in 20 (74.1%) of 27 HER2(+) patients, with a median overall survival (OS) of 58 mo, and in 60 (48.4%) of 124 HER2(-) patients, with a median OS of 133 mo. There were significant differences between the HER2-positive group and HER2-negative group with respect to both DFS [hazard ratio (HR): 1.69 (95%CI: 0.91-3.14); P = 0.048] and OS [1.95 (1.05-3.63); P = 0.0077], as shown in Figure 2A and B.

Figure 2 Kaplan-Meier survival analysis of disease-free survival and overall survival rates in relation to HER2 status. A: HER2 overexpression in rectal cancers is correlated with a shorter disease-free survival, P = 0.048; B: HER2 overexpression in rectal cancers is correlated with a shorter overall survival, P = 0.0077. DFS: Disease-free survival; OS: Overall survival.

DISCUSSION

HER2, also known as C-erbB-2, neu, or p185, is a member of the EGFR/ErbB family. It is a transmembrane protein encoded by the HER2 proto-oncogene, which participates in the signalling transduction pathway leading to cell growth and differentiation, potentially affecting the invasion and migration of tumour cells in the network of tumorigenesis. Previous studies have confirmed that HER2 is one of a predictors for poor prognosis in breast cancer, gastric cancer, endometrial cancer, and other tumours, in which HER2 overexpression is associated with poorer tumour biological properties[13-17]. In recent years, HER2 overexpression has also been found in CRC tumours; however, the data reported in the literature vary greatly, with positivity rates of approximately 2.6%-17%[18-22]. The effects of HER2 on the prognosis of CRC are also controversial, and the relationship between HER2 and the prognosis of patients with CRC remains under discussion. Our study is a 10-year long-term follow-up report on the prognosis of HER2 overexpression in locally advanced rectal cancer, which is of great significance in exploring the role of HER2 in rectal cancer.

Correlation between HER2 and local/distant metastasis of rectal cancer

In our study, the results from the correlation analysis using the chi-square test revealed that there were no significant differences between HER2 status and age, sex, pre-CEA, T stage, LVI, and local recurrence, which was consistent with the majority of previous reported results. Furthermore, HER2 positivity was associated with pre-N(+) (P = 0.040) and distant metastasis (P = 0.002), which is consistent with our previous short-term results[12]. Similar to our results, a study involving 1645 cases of primary colorectal adenocarcinoma showed that HER2 overexpression was associated with lymph node metastasis[23]. In addition, logistic regression was used to verify the predictive effect of HER2 overexpression on distant metastasis in rectal cancer. Univariate and multivariate analyses demonstrated that HER2 overexpression was associated with distant metastasis. The risk of distant metastasis in HER2-positive patients was 4.222 times higher than that in HER2-negative patients (P = 0.003, OR = 4.222, 97.5%CI: 1.667-11.409). We propose that these results suggest that overexpression of HER2 may promote the aggressiveness of rectal cancer. As in breast and gastric cancers, HER2 might play an important role in local failure and distant metastasis in patients with rectal cancer, featuring a higher possibility of lymph node metastasis.

Our results also showed that two other risk factors predictive of distant metastasis of rectal cancer were elevated pre-CEA (P = 0.002, OR = 3.277, 97.5%CI: 1.543-7.163) and post-N(+) (P = 0.022, OR = 2.437, 97.5%CI: 1.143-5.308). Studies have shown that pre-CEA is associated with neoadjuvant treatment response and clinical outcomes in patients with rectal cancer as a predictor of poorer prognoses[24-27]. Similarly, LN(+) status after neoadjuvant therapy was related to higher tumour stages and poorer treatment responses, which are obviously related to the prognoses of patients[28]. Therefore, we assumed that these might explain the increased rate of distant metastasis in elevated pre-CEA and post-N(+) patients.

Correlation between HER2 and long-term DFS/OS

A few studies have suggested that HER2 overexpression is a poor prognostic factor for CRC, playing an important role in its progression[29-31]. In the PETACC-8 trial, 1689 patients with stage III colon cancer received postoperative adjuvant chemotherapy. The results showed that HER2 overexpression was associated with a shorter time to recurrence [HR: 1.55 (95%CI: 1.02-2.36) P = 0.04] and shorter OS [HR: 1.57 (0.99-2.5) P = 0.05][32]. A meta-analysis of 1761 CRC patients from 11 studies by Li et al[33] showed that HER2 overexpression was negatively correlated with OS, and similar results were obtained in different subgroups, although the authors admitted that this effect might not be significant. However, some studies have shown that HER2 overexpression is not associated with the prognosis of CRC. In a large-pool study, three randomised controlled trials (the QUASAR, FOCUS, and PICCOLO trials) were analysed. Within a total of 3256 CRC patients enrolled, the results showed that there was no correlation between HER2 overexpression and survival (either progression-free survival or OS)[34].

Some studies have shown that cytoplasmic HER2-positive patients are associated with longer survival as an independent risk factor in Duke C stage patients[35]. In our short-term results report (median follow-up: 38 mo), the association between HER2 positivity and survival (DFS or OS) did not reach statistical significance. These results may be related to the short follow-up period. In this long-term study, with a median follow-up time of 134 mo, more events of distant metastasis or local recurrence occurred: 18 of 27 HER2-positive patients (66.7%) experienced disease progression, including 18 distant metastases and 2 Local recurrence events. There were significant differences between the HER2-positive and HER2-negative groups with respect to both DFS [HR: 1.69 (95%CI: 0.91-3.14); P = 0.048] and OS [HR: 1.95 (95%CI: 1.05-3.63); P = 0.0077] (Figures 1 and 2). The median DFS of HER2-positive patients was 43 mo. Of the 124 HER2-negative patients, 57 (46.0%) had distant metastases or recurrence events. Death occurred in 20 of 27 (74.1%) HER2-positive patients and 60 of 124 (48.4%) HER2-negative patients. The median OS was significantly shorter in HER2-positive patients than in HER2-negative patients (58 mo vs 133 mo). We hypothesis that HER2 overexpression, as a proto-oncogene, may be associated with increased tumour recurrence and poor prognosis. One explanation might be related to HER2’s inhibition of tumour cell apoptosis, resulting in tumour cell proliferation and acceleration of tumour aggressiveness. This suggests that, in order to pay special attention to local recurrence or distant metastasis, regular postoperative follow-up might be particularly important for patients with HER2-positive diseases, thereby improving their long-term survival. It can also provide possible suggestions for the enhanced treatment of these patients (combined with targeted therapy).

Correlation between HER2 and anti-HER2 targeted therapy

Recurrence and distant metastasis have been the main causes of treatment failure for locally advanced rectal cancer, despite many efforts having been made. The effect of anti-HER2 targeted drugs, such as trastuzumab and lapatinib, on breast cancer with high HER2 expression has been confirmed by several international multicentre open randomised controlled studies[36,37]. Studies have shown that metastatic CRC with wild-type RAS and HER2 overexpression might benefit from HER2 dual-targeted therapy[38,39]. Other studies have shown that trastuzumab, a HER2 inhibitor, can inhibit colony formation in colon cancer cells and reduce the viability of CRC cells in vitro[40]. Although this study did not involve targeted therapy, the results demonstrated that HER2-positive patients were associated with a higher rate of distant metastasis, which was related to poorer DFS and OS. These findings imply that more active treatments for patients with HER2-positive rectal cancer with distant metastasis or locally recurrent diseases are warranted, such as chemotherapy combined with single-targeted or even dual-targeted therapy. Moreover, HER2 overexpression in CRC may not only be an important prognostic determinant but also a potential therapeutic factor; however, this needs to be investigated further. Given that HER2-positive tumour cells are more likely to be aggressive in nature, anti-HER2 treatment may be beneficial for improving patient outcomes.

This study had a few limitations. The results showed that 27 (17.9%) patients were confirmed to be HER2-positive, a rate higher than that reported in most previous studies. However, this result is still considerably consistent with the results of previous studies. Most studies demonstrated that the variability of HER2 positivity might be caused by non-uniform judgment criteria, cohort heterogeneity, small study populations, different regimes of preoperative chemoradiotherapy, antibody selection methods, staining platforms, and so on[41]. In this study, all patients underwent rigid proctoscopy before neoadjuvant therapy, and a sufficient amount of tumour tissue was obtained. All samples were evaluated using immunohistochemistry, and the evaluation criteria were similar to those used when evaluating HER2 expression in gastric cancer. Two experienced pathologists independently performed the blind examinations and scoring. For controversial results, the FISH method was used to verify the accuracy of the results and exclude the possibility of false positives. A possible reason for the high rate of positivity is the potential selection bias resulting from an inadequate number of patients from a relatively larger HER2-negative population. However, we believe that due to the higher aggressiveness and poor prognoses of HER2-positive patients, our results may be more meaningful when compared with the results of these negative patients.

CONCLUSION

In conclusion, a considerable proportion of patients with rectal cancer showed HER2 overexpression. HER2 overexpression plays an important role in rectal cancer, which may promote the aggressiveness of rectal cancer and may be a potential prognostic biological predictor. For rectal cancer patients receiving preoperative neoadjuvant radiotherapy, HER2 overexpression predicts lymph node metastasis and distant metastasis and is associated with worse long-term DFS and long-term OS.

ARTICLE HIGHLIGHTS

Research background

Predictive factors for long-term survival in locally advanced rectal cancer remained controversial. The roles of HER2 over-expression was still under discussion.

Research motivation

The effects of HER2 over-expression on the long-term survival was investigated in this prospective cohort study.

Research objectives

The associations between clinico-pathological factors and long-term survival were evaluated.

Research methods

Categorical variables were assessed using the Chi square (2 × 2) or Fisher's exact test (2 × C), when applicable. Multivariate analysis was performed using a binary logistic regression model (forward: LR). Survival analysis was performed by the Kaplan-Meier method, and the differences between groups were verified by log-rank test.

Research results

The immunohistochemical results showed that HER2 over-expression was detected in 16.6% (25/151) of the tissue samples. HER2 positivity was associated with the pre-treatment N(+) stage (Pre-N) (P = 0.040) and the distant metastasis (P = 0.002). There were significant differences between HER2 positive group and HER2 negative group with respect to both disease-free survival (DFS) [hazard ratio: 1.69 (95% confidence interval: 0.91-3.14); P = 0.048] and overall survival (OS) [1.95 (1.05-3.63); P = 0.0077].

Research conclusions

A considerable part of rectal cancer patients showed HER2 overexpression. HER2 overexpression plays an important role in rectal cancer, which may promote the aggressiveness of rectal cancer, and it may be a potential prognostic biological predictor. For those rectal cancer patients receiving preoperative neoadjuvant radiotherapy, HER2 overexpression predicts lymph node metastasis and distant metastasis, and it is associated with worse long-term DFS and long-term OS.

Research perspectives

For rectal cancer patients, with HER2 over-expression, conventional treatment combined with targeted therapy might be of help.

ACKNOWLEDGEMENTS

We gave special thanks to faculty members (Professor Gu J, Professor Wu AW, etc.) of Gastro-intestinal center Ward III, Beijing Cancer Hospital.