Obese diet-induced mouse models of nonalcoholic steatohepatitis-tracking disease by liver biopsy

Table 1 Effect of Amylin liver nonalcoholic steatohepatitis model diet on metabolic parameters, non-alcoholic fatty liver disease activity score/fibrosis stage, body weight/composition and liver weight

	Lean chow n = 9	DIO-NASH n = 12	ob/ob chow n = 8	ob/ob NASH n = 10
Baseline plasma ALT (U/L)	30.7 ± 0.8	133.6 ± 16.3	207.0 ± 58.7	577.4 ± 43.4d,f
Terminal plasma ALT (U/L)	31.5 ± 2.9	126.1 ± 19.8	249.7 ± 47.4	670.0 ± 59.0d,f
Baseline plasma AST (U/L)	46.5 ± 2.2	134.8 ± 11.6b	174.2 ± 41.1	436.7 ± 36.8d,f
Terminal plasma AST (U/L)	139.0 ± 28.2	213.8 ± 31.6	338.9 ± 87.7	552.6 ± 49.5c,f
Baseline plasma TC (mmol/L)	2.1 ± 0.1	6.8 ± 0.3b	3.5 ± 0.2	10.4 ± 0.9d,f
Terminal plasma TC (mmol/L)	2.3 ± 0.1	6.7 ± 0.4b	4.4 ± 0.2	10.8 ± 0.6d,f
Baseline plasma TG (mmol/L)	0.7 ± 0.1	0.9 ± 0.1	1.1 ± 0.2	0.8 ± 0.1
Terminal plasma TG (mmol/L)	0.8 ± 0.1	1.0 ± 0.1	1.3 ± 0.1	0.7 ± 0.1d
OGTT-AUC	1104 ± 49.7	1217 ± 39.9	1612 ± 173.4	1319 ± 61.4
Fasting blood glucose (mmol/L)	7.3 ± 0.3	7.6 ± 0.2	8.1 ± 0.3	7.6 ± 0.3
Plasma insulin (pmol/L)	30.6 ± 6.7	97.4 ± 18.3	1189 ± 94	567.3 ± 123d,e
Baseline steatosis score (0-3)	0	2.7 ± 0.3b	1.8 ± 0.2	2.7 ± 0.3
Baseline fibrosis stage (0-4)	0	1.8 ± 0.2	0.25 ± 0.2	2.7 ± 0.3d,f
Terminal NAFLD activity score (0-8)	0	4.7 ± 0.4b	2.4 ± 0.3	6.3 ± 0.2d
Terminal steatosis score (0-3)	0	2.8 ± 0.1b	2.1 ± 0.2	2.1 ± 0.2
Terminal inflammation score (0-3)	0	1.4 ± 0.2b	0.3 ± 0.2	2.4 ± 0.2d
Terminal ballooning degeneration score (0-2)	0	0.4 ± 0.1	0	0.9 ± 0.1d
Terminal fibrosis stage (0-4)	0	1.2 ± 0.2a	0.1 ± 0.1	3.0 ± 0.2d,e
Terminal steatosis (% area)	5.4 ± 0.5	33.9 ± 2.6b	29.5 ± 2.3	41.2 ± 1.0c
Terminal fibrosis (% area)	0.3 ± 0.1	1.1 ± 0.2	1.2 ± 0.2	4.9 ± 0.7d,e
Terminal BW (g)	28.0 ± 0.3	39.1 ± 1.1	59 ± 1.1	54.8 ± 0.8e
Terminal lean tissue mass (g)	14.6 ± 0.8	18.8 ± 0.5b	19.9 ± 0.9	17.3 ± 0.4c
Terminal lean tissue mass (% of BW)	49.6 ± 2.6	47.7 ± 1.5	33.6 ± 1.1	31.9 ± 0.6f
Terminal fat tissue mass (g)	1.5 ± 0.1	8.1 ± 0.7b	25.1 ± 0.7	22.5 ± 0.5c,f
Terminal fat tissue mass (% of BW)	5.1 ± 0.5	20.0 ± 1.3b	42.3 ± 0.8	41.5 ± 0.5f
Liver weight (g)	1.1 ± 0.1	2.5 ± 0.3b	2.9 ± 0.2	5.4 ± 0.2d,f
Liver weight (% of BW)	3.9 ± 0.3	6.3 ± 0.6b	4.9 ± 0.3	9.9 ± 0.3d,f

^aP < 0.05 vs lean chow,

^bP < 0.01 vs lean chow,

^cP < 0.05 vs ob/ob chow,

^dP < 0.01 vs ob/ob chow,

^eP < 0.05 vs DIO-NASH and

^fP < 0.01 vs DIO-NASH. NASH: Nonalcoholic steatohepatitis; ALT: Alanine aminotransferase; AST: Aspartate aminotransferase; BW: Body weight; OGTT: Oral glucose tolerance test; AUC: Area under the curve.