Melatonin attenuates cisplatin-induced HepG2 cell death via the regulation of mTOR and ERCC1 expressions

doi:10.4254/wjh.v6.i4.230

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World Journal of Hepatology

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1948-5182

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Hepatol. Apr 27, 2014; 6(4): 230-242
Published online Apr 27, 2014. doi: 10.4254/wjh.v6.i4.230

Table 1 Genes of interests and the primer pairs for polymerase chain reaction

Genes	Function	Primers sequence (5'-3')	Size (bp)
Bax	Pro-apoptosis	F- AAAGCTAGCGAGTGTCTCAAGCGC	366
Bax	Pro-apoptosis	R-TCCCGCCACAAAGATGGTCACG	366
Bcl-2	Anti-apoptosis	F-TTGTGGCCTTCTTTGAGTTCG	332
Bcl-2	Anti-apoptosis	R-TACTGCTTTAGTGAACCTTTT	332
mTOR	Autophagic inhibition	F-TCTCATGGGCTTCGGAACAA R-GTGAAGGCAGAAGGTCGGAA	318
ERCC1	DNA repair	F-CCCTGGGAATTTGGCGACGTAA R-CTCCAGGTACCGCCCAGCTTCC	273
GAPDH	House keeping	F-CATCACCATCTTCCAGGAGC	307
GAPDH	House keeping	R-CATGAGTCCTTCCACGATACC	307

mTOR: Mammalian target of rapamycin; ERCC1: Excision repair cross complementary 1; GAPDH: Glyceraldehyde-3-phosphate dehydrogenase.

Citation: Bennukul K, Numkliang S, Leardkamolkarn V. Melatonin attenuates cisplatin-induced HepG2 cell death via the regulation of mTOR and ERCC1 expressions. World J Hepatol 2014; 6(4): 230-242
URL: https://www.wjgnet.com/1948-5182/full/v6/i4/230.htm
DOI: https://dx.doi.org/10.4254/wjh.v6.i4.230