Improved cryopreservation of human hepatocytes using a new xeno free cryoprotectant solution

Table 1 Demographic data and viability for patient samples used

Liver	Gender	Age(yr)	Viability(%)	Diagnosis
L1	F	43	75	Deceased donor, head trauma
L2	F	16	74	Deceased donor, head trauma
L3	M	60	83	Deceased donor, anoxia
L4	M	55	73	Deceased donor, head trauma
L5	M	46	84	PSC, CCC
L6	M	72	76	Colorectal metastasis
L7	M	36	80	PSC, CCC
L8	M	49	78	CCC
L9	M	69	68	Gallbladder cancer
L10	F	60	70	Gallbladder cancer
L11	F	65	75	CCC
L12	F	73	83	Colorectal metastasis
L13	F	62	76	Colorectal metastasis

L1-L9: The 9 liver samples used in this study; M: Male; F: Female; PSC: Primary biliary cirrhosis; CCC: Cholangiocellular carcinoma.

Table 2 Comparison of controlled rate freezer vs a polystyrene box in ordinary -70 °C freezer

	Fresh	PSB	CRFa
L1	75	22	6
L2	74	38	22
L3	83	42	18
L4	73	40	37

Data represent percent ± SD of viable cells using trypan blue exclusion method.

^aP < 0.01 vs fresh. CRF: Controlled rate freezer; PSB: Polystyrene box.