Yinhuang granule alleviates carbon tetrachloride-induced liver fibrosis in mice and its mechanism

Table 1 List of Reagents

Reagents	Company
Anti-Lamin B1	Hangzhou Hua-An Biotechnology Co., Ltd. (Hangzhou, China)
Anti-α-SMA	Cell Signaling Technology (Danvers, MA, United States)
Anti-NRF1	Cell Signaling Technology (Danvers, MA, United States)
Anti-β-actin	Cell Signaling Technology (Danvers, MA, United States)
Anti-Nrf2	Gene Tex Inc. (Alton Parkway lrvine, CA, United States)
Anti-GCLC	Santa Cruz (Santa Cruz, CA, United States)
Anti-NQO1	Abways Technology, Inc. (Shanghai, China)
Anti-GCLM	Abways Technology, Inc. (Shanghai, China)
Anti-PGC1	Abcam (Shanghai, China)
Kits for detecting ALT/AST activity	Nanjing Jiancheng Bioengineering Institute (Nanjing, China)
Kits for detecting liver hydroxyproline content	Nanjing Jiancheng Bioengineering Institute (Nanjing, China)
Kits for detecting hepatic GSH	Nanjing Jiancheng Bioengineering Institute (Nanjing, China)
Kits for detecting protein carbonylation amount	Nanjing Jiancheng Bioengineering Institute (Nanjing, China)
Kits for detecting SOD activity	Nanjing Jiancheng Bioengineering Institute (Nanjing, China)
ATP content	Beyotime Biotech (Shanghai, China)
ELISA kits	RapidBio (West Hills, CA, United States)
mitochondrial DNA extraction	Sangon Biotech (Shanghai, China)
NE-PER nuclear and cytoplasmic extraction reagents	Thermo Fisher Scientific (Waltham, MA, United States)
BCA Protein Assay Kits	Thermo Fisher Scientific (Waltham, MA, United States)
PrimeScript Master Mix	Takara (Shiga, Japan)
SYBR Premix Ex Taq	Takara (Shiga, Japan)
Trizol reagent	Life Technology (Carlsbad, CA, United States)
2’,7’-dichlorodihydrofluorescein diacetate (H2DCFDA)	Life Technology (Carlsbad, CA, United States)

Other reagents unless indicated were obtained from Sigma Chemical Co. (St. Louis, MO, United States).

Table 2 List of Primers for real-time polymerase chain reaction

Target	Primer	Sequence (5'-3')
Col1a1	FP	TGACTGGAAGAGCGGAGAGT
	RP	GACGGCTGAGTAGGGAACAC
Col3a1	FP	ATGGGTTTCCCTGGTCCTAA
	RP	TGCCTTGTAATCCTTGTGGA
Fn1	FP	AGAACCAGAGGAGGCACAAG
	RP	CCGTGTAAGGGTCAAAGCAT
Tgfb1	FP	TGCCCTCTACAACCAACACA
	RP	GTTGGACAACTGCTCCACCT
Acta2	FP	GGGAGTAATGGTTGGAATGG
	RP	GGTGATGATGCCGTGTTCTA
TNFα	FP	AGGCACTCCCCCAAAAGAT
	RP	CAGTAGACAGAAGAGCGTGGTG
IL-1β	FP	AGTTGACGGACCCCAAAAG
	RP	CTTCTCCACAGCCACAATGA
IL-6	FP	ACAAAGCCAGAGTCCTTCAGAGAG
	RP	TTGGATGGTCTTGGTCCTTAGCC
iNOS	FP	CAGGCGGTGCCTATGTCTC
	RP	CAGCTGGGCTGTACAAACCTT
Nqo1	FP	CTCGTGGAGACGCTTTACAT
	RP	CGTTTCTTCCATCCTTCCAG
Gclc	FP	CGGAGGAACGATGTCTGAGT
	RP	CTGGGGAATGAAGTGATGGT
Gclm	FP	CAATGACCCGAAAGAACTGC
	RP	CAATGACCCGAAAGAACTGC
Actin	FP	TTCGTTGCCGGTCCACACCC
	RP	GCTTTGCACATGCCGGAGCC
18s	FP	CGCGGTTCTATTTTGTTGGT
	RP	AGTCGGCATCGTTTATGGTC
ND1	FP	CTAGCAGAAACAAACCGGGC
	RP	CCGGCTGCGTATTCTACGTT

FP: Forward primer; RP: Reverse primer; TNF: Tumour necrosis factor; IL: Interleukin; iNOS: Inducible nitric oxide synthase.