Ipragliflozin-induced improvement of liver steatosis in obese mice may involve sirtuin signaling

Table 1 Sequences of the primers used for reverse transcription-polymerase chain reaction

	Forward primer sequence	Reverse primer sequence
SIRT1	5’-GTA AGC GGC TTG AGG G-3’	5’-TTC GGG CCT CTC CGT A-3’
PGC-1α	5’-TTG ACT GGC GTC ATT CGG GAG-3’	5’-ATC TGG GCA AAG AGG CTG GTC-3’
PPARα	5’-AGG AAG CCG TTC TGT GAC AT-3’	5’-TTG AAG GAG CTT TGG GAA GA-3’
FGF21	5’-AGA TCA GGG AGG ATG GAA CA-3’	5’-TCA AAG TGA GGC GAT CCA TA-3’
FAS	5’-ACC ACT GCA TTG ACG GCC GG-3’	5’-GGG TCA GGC GGG AGA CCG AT-3’
ACC	5’-GGG CAC AGA CCG TGG TAG TT-3’	5’-CAG GAT CAG CTG GGA TAC TGA-3’
ACOX1	5’-TGG TAT GGT GTC GTA CTT GAA TGA C-3’	5’-AAT TTC TAC CAA TCT GGC TGC AC-3’
ACS	5’-AAA GAT GGC TGG TTA CAC ACG-3’	5’-CGA TAA TCT TCA AGG TGC CAT T-3’
CPT1	5’-CCC TGG GCA TGA TTG CAA-3’	5’-AAG AGG ACG CCA CTC ACG AT-3’
CPT2	5′-CAG ACA GTG GCT ACC TAT GAA TCC T-3′	5′-TGG TCA GCT GGC CAT GGT ATT TGG A-3′
Nox2	5′-GAA AAC TCC TTG GGT CAG CAC T-3′	5′-ATT TCG ACA CAC TGG CAG CA-3′
GPx-1	5′-TTA CAT TGT TTG AGA AGT GCG A-3′	5′-CAA AGT TCC AGG CAA TGT C-3′
SOD-1	5′-CAT TCC ATC ATT GGC CGT-3′	5′-TCA GAC CAC ACA GGG AAT GTT TA-3′
SOD-2	5′-TGT ATA TCT CTG GAG AAC TGG AC-3′	5′-GGC CCT CTT GTG ACT GTA A-3′
IL-1β	5′-AAA CGG TTT GTC TTC AAC-3′	5′-ATG GTG AAG TCA ATT ATG TC-3′
F4/80	5′-CAT CTT GCT GGA GAC TGT-3′	5′-CTG CCA AGT TAA TGG ACT CA-3′
β-actin	5’-AGC CTT CCT TCT TGG GTA-3’	5’-GAG CAA TGA TCT TGA TCT TC-3’

ACC: Acetyl-CoA carboxylase; ACOX1: Acyl-CoA oxidase 1; ACS: Acyl-CoA synthetase; CPT1: Carnitine palmitoyltransferase 1; CPT2: Carnitine palmitoyltransferase 2; FAS: Fatty acid synthase; FGF21: Fibroblast growth factor-21; GPx-1: Glutathione peroxidase 1; IL-1β: Interleukin-1β; Nox2: NADPH oxidase 2; PGC-1α: Peroxisome proliferator-activated receptor γ coactivator 1α; PPARα: Peroxisome proliferator-activated receptor α; SIRT1: Sirtuin 1; SOD-1: Superoxide dismutase 1; SOD-2: Superoxide dismutase 2.