Hepatitis E virus infection-triggered intrahepatic cholestasis: A case report

Figure 1 Longitudinal course of liver tests and genetic variants. A: Clinical course of Patient 1 and Patient 2 showing bilirubin levels (black line) and gamma-glutamyltransferase (GGT) levels (gray line) over time (in days). B: Genetic variants determined by next-generation sequencing and genotyping. Variants are annotated with corresponding minor allele frequencies (MAFs), obtained from ensembl.org, and linked to previously published references (Supplementary Table 1). GGT, gamma-glutamyl transferase; MAF, minor allele frequency; ABCB4: ATP-binding cassette 4; ABCB11: ATP-binding cassette, sub-family B member 11; ATPB1: ATPase phospholipid transporting 8B1; MYO5B: Myosin VB; FXR, farnesoid X receptor; n/a: Not available.

Figure 2 Histology of liver biopsies. Representative histological staining of liver biopsy samples from both patients. The following stainings were performed: BSEP: Bile salt export pump; CK7: Cytokeratin 7; HE: Hematoxylin and eosin; MAS: Masson’s trichrome; MDR3: Multidrug resistance protein 3. Long scale bar: 100 µm; Short scale bar in inlay: 40 µm (BSEP and MDR3 staining).