Epigenetic basis of hepatocellular carcinoma: A network-based integrative meta-analysis

Figure 1 Flow chart. A: Workflow of Data collection, analysis and database compiling; B: Bioinformatics flow for selecting differentially methylated CpG sites from HCC meta-analysis; C: Bioinformatics flow for selecting differentially methylated genes from HCC meta-analysis. HCC: Hepatocellular carcinoma.

Figure 2 Networks associated with differentially methylated CpG sites in the promoter regions of genes. A: Organismal development, organismal injury and abnormalities, cellular development; B: Lipid metabolism, small molecule biochemistry, cell death and survival; C: Cell-to-cell signaling and interaction, drug metabolism, small molecule biochemistry.

Figure 3 Networks associated with differentially methylated CpG sites in body of the gene. A: Cellular assembly and organization, cellular function and maintenance; B: Drug metabolism, glutathione depletion in liver, small molecule biochemistry.

Figure 4 Venn diagram intersecting three lists: (1) reported differentially methylated genes in HCC from studies not providing information on the corresponding CpG sites; (2) identified differentially methylated genes in HCC corresponding to CpG sites in promoter; and (3) identified differentially methylated genes in HCC corresponding to CpG sites in body of the gene.