The present study aimed to evaluate the effect of the inclusion in lamb diet of hazelnut skin (H diet), extruded linseed (L diet), or a combination thereof (HL diet) on the oxidative stability of cooked and in vitro digested lamb meat compared to a basal diet (C diet). A significant decrease of 46.1 % and 40.9 % in lipid hydroperoxides was attended after in vitro digestion in lamb meat from the L and H diets with respect to C diet. Moreover, the HL diet was the most effective in decreasing the TBA-RS value after intestinal digestion (23.3 % decrease compared to C diet). Five different phenolic- and four tocopherol-derived metabolites were identified whose amount was greater in meat from supplemented diets. Furthermore, the inclusion of hazelnut skin in the lamb diet resulted in higher amount of endogenous antioxidants (carnosine and reduced glutathione) in raw meat.

Many studies demonstrate boosting beef omega-3 content causes detrimental effects on product color stability and palatability. The study objective was to determine effects of feeding Great O Plus on ground beef fatty acid composition, color stability, and palatability. Ground Round (90/10) and Chuck (80/20) patties were produced from steers fed either a conventional finishing diet (CON;n = 14) or a conventional finishing diet containing a 10 % extruded proprietary flaxseed, wheat middling, and Nannochloropsis oculata algae blend (OMG; n = 14). To evaluate color stability, patties were displayed under retail conditions for 4 days with objective and subjective color measures collected every 12 h. Cooked patties were rated by both trained and consumer panels to evaluate palatability. Round and Chuck patties produced from steers fed Great O Plus increased (P < 0.01) alpha-linolenic acid content in both product types. Patties from the OMG treatment did not differ in color stability nor palatability measures when compared to CON patties (P < 0.15). When differences occurred, OMG patties had less visual discoloration, decreased lipid oxidation, and greater overall consumer palatability ratings (P < 0.09). While not directly measured in the present study, it is hypothesized the lack of detrimental effects on product color and palatability was due to a combination of the elevated antioxidant content of the Nannochloropsis oculata algae in Great O Plus and lack of measurable long-chain omega-3 fatty acid presence.

Endometriosis is a chronic, hormone-dependent disorder characterized by an inflammatory response. The disease affects approximately 10% of the general female population, with prevalence rates reaching 30-40% in women with dysmenorrhea and 50-60% in those experiencing infertility. In addition to pelvic pain and reproductive issues, gastrointestinal symptoms, such as acute abdominal pain, constipation, diarrhea, or alternating bowel habits, are frequently reported and can be highly disabling. Emerging evidence indicates that dietary patterns may modulate the inflammatory environment associated with endometriosis, potentially influencing symptom severity by affecting oxidative stress, estrogen metabolism, and levels of sex hormone-binding globulin (SHBG). Diets rich in antioxidants, polyunsaturated fatty acids (PUFAs), and vitamins D, C, and E-alongside the avoidance of processed foods, red meat, and animal fats-may offer beneficial effects. This narrative review explores the relationship between nutrition and endometriosis, emphasizing the therapeutic potential of dietary interventions as a complementary strategy. Notably, dietary approaches may serve not only to alleviate pain and improve fertility outcomes but also to reduce lesion growth and recurrence, particularly in patients seeking pregnancy or those unable to undergo hormonal therapy due to contraindications. Furthermore, nutritional strategies may enhance postoperative recovery and act as a viable first-line therapy when conventional treatments are not applicable. A total of 250 studies were initially identified through PubMed and Scopus. After removing duplicates and non-relevant articles, 174 were included in this review. Our findings underscore the urgent need for further studies to develop evidence-based, personalized nutritional interventions for managing endometriosis-related symptoms.

Protectin D1 (PD1) derived from docosahexaenoic acid (DHA) has shown promise in resolving inflammation. Mast cells are critical drivers of allergic inflammation, releasing inflammatory mediators such as histamine and pro-inflammatory cytokines. This study assesses the effectiveness of PD1 in counteracting mast cell-mediated allergic inflammation. In vivo, two well-established mouse models were employed: IgE-mediated passive cutaneous anaphylaxis (PCA) and ovalbumin-induced active systemic anaphylaxis (ASA). The oral administration of PD1 markedly suppressed PCA reactions, including ear swelling, plasma extravasation of Evans blue and mast cell degranulation. In the ASA model, oral PD1 administration dose-dependently alleviated hypothermia and reduced elevated serum levels of IgE, histamine, and IL-4. Mechanistic insights were gained through studies in the RBL-2H3 and primary mast cells derived from mouse bone marrow, where PD1 inhibited IgE-mediated degranulation and decreased intracellular calcium influx by blocking FcεRⅠ signaling pathways involving Lyn, Fyn, and Syk kinases. Additionally, PD1 suppressed pro-inflammatory cytokine production by inhibiting the activity of critical transcription factor; nuclear factor-κB. These findings suggest that PD1, a bioactive lipid derived from DHA, is a very promising therapeutic candidate for mast cell-derived allergic inflammation.

Polyunsaturated fatty acids (PUFAs) play roles in several physiological and pathophysiological processes, but their effects on reproductive function are controversial. The aim of the study was to investigate the effect of n-3 PUFA-rich fish oil and n-6-rich sunflower oil on sex hormone status, in vivo and in vitro uterine contractility, and endometrial remodeling. Female Sprague Dawley, Lister hooded, and Wistar rats were treated orally for 20 days with 1 mL of tap water, sunflower oil, or fish oil. Blood samples were taken for gonadotropic and sex hormone analysis. In vivo smooth muscle contractions were measured weekly by electromyography. Isolated uterine and cecal contractions were measured after sacrificing the animals. Endometrial remodeling was detected based on the presence of αvβ3 integrin by optical imaging. In Sprague Dawley rats, fish oil increased the LH level and progesterone/estradiol (P4/E2) ratio compared to the sunflower oil-treated group. Uterine contractions were reduced both in vitro and in vivo. Endometrial αvβ3 integrin activity was increased in the fish oil group. In Lister hooded rats, neither sunflower nor fish oil treatments modified the investigated parameters. However, in Wistar rats, both oils increased only the in vivo contractions and reduced the P4/E2 ratio, along with αvβ3 integrin fluorescence. n-3 PUFA-rich fish oil induces a breed-dependent effect on sex hormone status and uterine contractions in rats. The response to PUFA intake may vary significantly within a given species, which may have importance both in animal feeding and human nutrition.

In this study, we investigated the effects of nutritional supplementation as a strategy to mitigate the impacts of imidacloprid (neonicotinoid) on honey bees by using Ahiflower® (Buglossoides arvensis) seed-oil. This oil is rich in stearidonic-acid (SDA, 18:4n3), which is a precursor to eicosapentaenoic-acid (EPA) and docosahexaenoic-acid (DHA) that are known for their beneficial and protective effects. Specifically, we chronically fed newly emerged worker bees with sucrose syrup and pollen patties (control) that we supplemented with (i) imidacloprid (0.375 ng·μl-1), (ii) Ahiflower® oil (5 %) + imidacloprid (0.375 ng·μl-1), and (iii) Ahiflower® oil (5 %). Survival was recorded, and after 21 days, worker bees were sampled to measure mitochondrial respiration, ATP5A1 content, adenylate energy charge, lipid peroxidation in thorax as well as fatty acid composition and peroxidation index in whole bees. Our results indicate that (i) imidacloprid mostly hampers mitochondria, increases saturated fatty acids and decreases survival, (ii) oxidation of alternative substrates allows full recovery of mitochondrial respiration in the imidacloprid-treated group demonstrating mitochondrial flexibility, (iii) Ahiflower® oil in combination with imidacloprid partially restores mitochondrial respiration at the level of complexes I and II, restores fatty acid composition but fails to restore survival. These findings confirm the deleterious effects of imidacloprid on mitochondria while highlighting, for the first time, the potential benefits of Ahiflower® oil in mitochondrial function, though not on honey bee survival. In addition, this study highlights the importance of mitochondrial flexibility when organisms are exposed to toxicants at environmentally relevant levels.

This clinical study combined alanyl-glutamine and omega-3 polyunsaturated fatty acids (ω-3 PUFAs) to investigate the effects of parenteral nutrition on postoperative inflammation and nutritional status in patients with gastroduodenal perforation to provide a basis and support for the use of clinical immunonutrients. Patients with gastroduodenal perforations who underwent surgery between January 2018 and December 2023 were included. From the first to the seventh postoperative day, Group A (GA) received conventional postoperative nutrition with fat emulsion (20%), amino acids (17), and glucose (11%) injection; Group B (GB), building on GA's regimen, was additionally treated with 10 g/day of ω-3 PUFAs; and Group C (GC), expanding on GB's regimen, was additionally treated with 10 g/day of alanyl-glutamine. A total of 168 patients were included in the study, with 71 in GA, 30 in GB, and 67 in GC. Total protein and albumin (Alb) levels increased in all 3 groups, with GC showing a more significant increase compared to GB and GA (TP: 7.73 ± 5.00 vs 4.35 ± 5.85 vs 3.92 ± 5.07, P < .05; Alb: 4.07 ± 4.52 vs 1.79 ± 4.00 vs 2.11 ± 4.10, P < .05); C-reactive protein levels decreased in all 3 groups, with the most pronounced decrease in GC (93.71 ± 80.97 vs 72.04 ± 80.48 vs 55.79 ± 83.68, P < .05); the length of hospitalization and among the 3 groups was statistically significant (10.7 ± 2.27 vs 13.39 ± 4.66 vs 12.52 ± 3.46, P < .05), and GB was shorter than GA; the incidence of postoperative complications was significantly lower in GC than in other groups (P < .05). Parenteral nutrition supplemented with alanyl-glutamine and ω-3 PUFAs can increase postoperative total protein and Alb levels, thereby improving patient nutritional status, reducing the production of the inflammatory marker C-reactive protein, mitigating the inflammatory response, and decreasing the incidence of postoperative complications, thus improving patient prognosis.

Seaweed polysaccharides are effectively applied in food nano-encapsulation due to their biosafety and unique physical and chemical properties. Docosahexaenoic acid (DHA) of the polyunsaturated fatty acids (PUFAs) has been favored by people as an essential nutrient. However, DHA has defects such as easy oxidation, low solubility and low bioavailability. In this study, a novel DHA-loaded nanoparticle (DNP), comprising O/W-type nano-encapsulation systems (KCDNP and LAGDNP), was developed via the anti-solvent method using κ-carrageenan (KC) and low melting point modified agar (LAG) as wall materials, soy lecithin (SL) as the emulsifier, and octanoic acid glyceride (GTCC) as an assistant, achieving small particle sizes (300-700 nm) and high encapsulation efficiency (up to 92.56 %). The nano encapsulated system increases the water solubility of DHA to 3.5-4.5 mg/mL (originally <1 μg/mL). The particle size of NPs exhibits certain stability in different environments, and the peroxide value (POV) is significantly lower than that of traditional microcapsules (POV ≤ 4.92 meq/kg after 30 days). The combination of infrared spectroscopy and specific detergent treatment showed that intermolecular hydrogen bonds and electrostatic interactions were the main forces to form a stable system. In vitro simulated digestion showed that DHA in NPs had a slow-release rate (<27 %) in the gastric phase and a release rate of over 90 % in the intestinal phase, demonstrating good sustained release function and high bioavailability. Both KCDNP and LAGDNP NPs had good stability and lipid antioxidant activity, which would increase the application of DHA in functional foods and the high value of seaweed polysaccharides.

Alpha-linolenic acid (ALA, C18:3) is an essential fatty acid integral to human growth and development. Despite its significance, the ALA content in peanut seeds-a major global oilseed crop-is notably low. This study employed bioinformatics analysis, tissue expression, and promoter function evaluations to investigate AhFAD3, which encodes the microsomal omega-3 fatty acid desaturase that is directly responsible for ALA accumulation through converting linoleic acid (LA) to ALA. We identified the active AhFAD3 gene, AhFAD3-A01, with the functional protein encoded by AhFAD3-A01 localized in the endoplasmic reticulum (ER) and found to be pivotal in ALA synthesis in seeds. The low expression of AhFAD3 genes during the late stages of seed development, coupled with the specific activation by only AhFAD3-A01 and AhFAD3-B01 promoters in seeds, results in the low ALA levels in mature peanut seeds. To enhance ALA content, the constitutive promoter CaMV35S and the seed-specific promoter PAhWRI1 were utilized to overexpress AhFAD3-A01 in Arabidopsis and peanut. While the expression level of AhFAD3-A01 in PAhWRI1::AhFAD3-A01 transgenic Arabidopsis remained unchanged, it significantly increased under the CaMV35S::AhFAD3-A01 configuration, leading to an over a 40 % increase in ALA content of in T4 generation seeds, indicating that PAhWRI1 was unable to drive AhFAD3 overexpression in Arabidopsis. Similarly, the overexpression of AhFAD3-A01 using both promoters in peanuts resulted in enhanced expression and an increase in ALA content from 15.18 % to 30.65 % in CaMV35S::AhFAD3-A01 T1 generation seeds and from 11.23 % to 25.49 % in PAhWRI1::AhFAD3-A01 seeds. These results highlight the critical role of AhFAD3-A01 ALA synthesis in peanut seeds and provide a solid foundation for developing peanut varieties with elevated ALA content.

Polyunsaturated fatty acids (PUFAs) are essential for maintaining human health, playing a critical role in preventing cardiovascular diseases and promoting brain development. PUFAs can be synthesized through two independent biosynthetic pathways: the desaturase/elongase pathway and the PUFA synthase pathway. Among these, PUFA synthase is a crucial enzyme analogous to fatty acid synthase (FAS) and iterative polyketide synthase (PKS). Its high synthetic efficiency presents significant potential for enhancing fatty acid biosynthesis and expanding its applications. Recent in vitro and in vivo studies have progressively clarified the catalytic mechanisms of PUFA synthase, enabling the more precise engineering of this enzyme. This Review provides a comprehensive analysis of PUFA synthase, emphasizing its catalytic potential, engineering prospects, and expanding applications in fatty acid biosynthesis, offering novel insights for advancing its role in industrial and pharmaceutical innovation.

This study aimed to determine the effects of the Mediterranean diet (MD) and lycopene on the development of EAE and on inflammatory markers. In the 43-day study, 72 female C57BL/6 mice were randomly divided into eight groups according to whether they were EAE or naive (control) mice, fed a Western diet or a MD, and whether they received lycopene. During the study, mice were fed ad libitum, and lycopene groups were given 10 mg/kg/day lycopene per mouse every other day for 28 days in oral gavage. The mice were scored for EAE, sacrificed and their spleen, lymph nodes, and spinal cords were removed. We observed slightly delayed EAE onset in the MD-Lyc group compared to the others, and the EAE clinical scores were also lower than in the other groups. T-cell counts in the spleen and lymph nodes of the MD-Lyc group were significantly lower than in other groups. The production of IFN-γ and IL-22 was higher than in the other groups. IL-17 A cytokine produced in the spleen was lower in the MD-Lyc group than in the other groups. In addition, the highest myelination score was seen in the MD-Lyc group. MD-Lyc group also had a unique microbiome profile compared with the remaining groups. In summary, MD and lycopene administration positively impacted EAE scores and myelination. However, more comprehensive studies at the in vitro and in vivo levels are needed to reveal the effect of this intervention on cell numbers in the CNS.

Omega-3 fatty acids play a significant role in immunomodulation, with nutrigenomic approaches highlighting their impact on gene expression related to immune responses. Research indicates that omega-3 fatty acids can modulate inflammatory pathways, potentially reducing chronic inflammation and enhancing immune function. This review discusses the intersection of nutrigenomics and nutriepigenomics, focusing on how omega-3 fatty acids influence gene expression, immune function, and overall health. The immune system is a complex network responsible for defending the body against pathogens and maintaining internal balance. Comprised of innate and adaptive immunity, the system involves various cells, tissues, and organs working together to combat infections and prevent diseases. Omega-3 polyunsaturated fatty acids (PUFAs), particularly eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), play a significant role in modulating the immune system. These fatty acids influence immune cell function, membrane fluidity, and signaling processes, enhancing immune responses and reducing inflammation. Furthermore, EPA and DHA affect several signaling pathways, reducing the expression of proinflammatory cytokines and inhibiting nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) activation, a critical transcription factor in the inflammatory response. Additionally, they activate PPAR-γ, further diminishing inflammatory gene expression. As precursors to specialized proresolving lipid mediators, EPA and DHA help shift the lipid mediator profile from proinflammatory to antiinflammatory derivatives, thus aiding in the resolution of inflammation.

Silkie chickens are a unique breed renowned for their pigmentation, food and medicine homology properties, and distinctive appearance, making them highly valuable in exhibitions, as pets, in medicinal cuisine, and as a model for melanin research. Despite their vast potential, the growing volume of publications and patents related to Silkie chicken highlights the critical need for systematic organization, summarization, and analysis of this wealth of information. For the first time, this study employs bibliometric tools to summarize and analyze 114 years of research on Silkie chicken. Our study demonstrates that academic studies primarily focus on their nutritional value, melanin production, and genetic mechanisms, while patents emphasize food formulations, breeding methods, and purebred identification. Although there has been significant growth in publications and citations since 2001, international collaboration remains limited. This study presents the need for integrated and multidisciplinary research to unlock the full potential of Silkie chicken and provides a foundational framework for future studies and applications.

For two decades, the National Institute of Standards and Technology (NIST) and the National Institutes of Health Office of Dietary Supplements have collaborated to develop dietary supplement-matrix reference materials. During the first decade, NIST developed over 20 botanical and non-botanical dietary supplement Standard Reference Materials (SRMs®) using multiple analytical techniques to assign values for selected marker compounds and toxic elements. In the past decade, NIST has expanded the scope of materials available, and other producers of certified reference materials (CRMs) have joined to provide a limited number of additional materials. This review describes briefly the first decade in the development of CRMs for dietary supplements, primarily botanical dietary supplement ingredients (e.g., ginkgo, green tea, saw palmetto, St. Johns' wort, botanical oils, berries, and soy) and a popular multivitamin/multimineral (MVM) SRM. We discuss the analytical challenges in producing these materials and how these materials established a model for the next generation of CRMs. The second generation of dietary supplement CRMs/RMs, consisting primarily of botanical matrices, calibration solutions, and new and replacement MVM CRMs, is discussed in greater detail including improvements based on experiences from the first decade and potential future needs and developments in this emerging reference material research sector.

Lipid metabolism disorder serve as a critical starting point for the development of chronic non-communicable diseases (NCDs). Eicosapentaenoic acid (EPA) and Docosahexaenoic acid (DHA) are known for their lipid-lowering properties, but few studies have revealed their differences from the perspective of skeletal muscle endocrinology. Myogenic IL-6 has garnered significant attention for its role in energy distribution. The primary aim of this study was to investigate the effects and mechanisms of EPA and DHA on myogenic IL-6 and lipid metabolism disorders in mice, and to clarify the association between the alleviation of lipid metabolism disorders and myogenic IL-6 mediated by EPA/DHA. We found that EPA and DHA not only prevented high-fat diet-induced lipid metabolism disorders, but also up-regulated the expression of myogenic IL-6 by activating TRPV1/Ca2+ signaling in skeletal muscle. However, the lipid metabolism prevention effect mediated by EPA was weakened after knockout gene of myogenic IL-6, with its body weight and body fat increased and a large amounts of lipid deposited in the blood, liver, and adipocytes. Meanwhile, there no significantly differences of AMPK/STAT3 signaling in adipose tissue between groups after knockout gene of myogenic IL-6. Based on the results above, we concluded that EPA and DHA can stimulate the production of myogenic IL-6 through TRPV1/Ca2+ signaling in skeletal muscle. The prevention effect of lipid metabolism disorders by EPA, but not DHA, relies on myogenic IL-6, with the underlying mechanism may involving the enhancement of AMPK/STAT3 signaling mediated by myogenic IL-6 in adipose tissues.

Polyunsaturated fatty acids (PUFAs) are known to have beneficial effects. In particular, the consumption of omega-3 PUFAs has recently increased because of their effects on human health. Previous studies have investigated the activity of alpha-linolenic acid (ALA; C:18 omega-3) in metabolism and inflammation models. In a murine model of colitis, treatment with ALA effectively reduced inflammation. Previously, our research group identified the protective action of docosahexaenoic acid against gastric damage caused by nonsteroidal anti-inflammatory drugs. The present study aimed to examine the impact of ALA in an indomethacin-induced gastric injury model and to determine its antioxidant activity in gastric tissue. Female Wistar rats were administered ALA over 10 days (20 mg/kg, orally). Two hours after the final ALA administration, the rats were given indomethacin (30 mg/kg, orally) to induce gastric injury. After 3 h, the rats were euthanized, and each stomach lesion was measured to determine the total damage. Stomach tissue samples were collected for the analysis of various antioxidant indicators. The results show ALA's gastroprotective effect following 10-day administration. ALA treatment significantly reduced gastric reactive oxygen species and malondialdehyde levels in the indomethacin-induced injury group. Moreover, ALA treatment decreased the levels of nitric oxide, myeloperoxidase, leukotriene B4, and increased glutathione following indomethacin administration. These results suggest that the gastroprotective effects of ALA are likely attributed to its role in the antioxidant pathway in indomethacin-induced gastric injury.

Postoperative wound infections remain a significant challenge in gastrointestinal surgery, with substantial implications for prolonged hospital stays and healthcare costs. Immunonutrition with n-3 polyunsaturated fatty acids (PUFAs) offers potential preventive benefits through modulation of inflammatory responses. This study aimed to explore the impact of enteral nutritional enriched with n-3 PUFAs on wound infection in patients undergoing gastrointestinal surgery.

A thorough search was carried out, from databases to December 2023, across Cochrane Library, Embase, CNKI, Google Scholar, Wanfang, and PubMed on randomized controlled trials (RCTs) examining the impacts of enteral nutritional enriched with n-3 PUFAs for treatment of patients with gastrointestinal surgery. Two independent researchers conducted literature screening, data extraction, and quality assessments. The primary outcome measures were wound infection and complications. Statistical analysis was conducted in Stata 17.0 and RevMan 5.4 software.

Fifteen RCTs involving 1442 patients who underwent gastrointestinal surgery were included. The analysis revealed the application of enteral nutritional enriched with n-3 PUFAs markedly decreased the occurrence of complications (odds ratio [OR] = 0.56, 95 % confidence intervals [CI]: 0.44-0.71, P < 0.001) and wound infection (OR = 0.68, 95 %CI: 0.47-0.98, P = 0.04) in patients who underwent gastrointestinal surgery.

This study demonstrates that postoperative support with enteral nutritional enriched with n-3 PUFAs can effectively decrease the occurrence of wound infections and postoperative complications following gastrointestinal surgery. These findings suggest that n-3 PUFAs supplementation should be considered as a standard component of perioperative nutritional protocols to improve surgical outcomes in patients undergoing gastrointestinal procedures.

This study aimed to evaluate the effect of Omega-3 Fatty Acids (ω-3 PUFAs) on endometriosis.

The authors conducted a comprehensive search of the PubMed, Embase, Cochrane Library and Web of Science databases, focusing exclusively on Randomised Controlled Trials (RCTs) to study the impact of ω-3 PUFAs on endometriosis. The included studies were assessed for methodological quality using the Cochrane bias risk assessment tool and analyzed using data analysis software.

The search yielded five RCTs conducted between the database's inception and July 2023, with a total sample size of 424 patients with endometriosis. The meta-analysis results showed no statistically significant effects of ω-3 Polyunsaturated Fatty Acids (PUFAs) on pain (Mean Difference [MD = -0.387], 95 % Confidence Interval [95 % CI -1.742-0.967], I2 = 93.3 %, z = 0.56, p = 0.575), sexual activity (MD = 0.143, 95 % CI -0.210-0.497, I2 = 0 %, z = 0.79, p = 0.427), pain intervention (MD = -0.216, 95 % CI -0.717-0.285, I2 = 0.0 %, z = 0.84, p = 0.399), catastrophic thinking (MD = 0.158, 95 % CI -0.315-0.632, I2 = 0.0 %, z = 0.66, p = 0.512) and the 12-item short form health survey (MD = 0.001, 95 % CI -0.053-0.503, I2 = 0.0 %, z = 0.00, p = 1.000), which were all statistically insignificant. However, ω-3 PUFAs appeared to reduce the inflammatory response in patients with endometriosis (MD = -5.20, 95 % CI -6.21--4.20, I2 = 0 %, z = 10.13, p < 0.001).

Based on the available evidence, ω-3 PUFAs may reduce the inflammatory response in patients with endometriosis, specifically by decreasing levels of pro-inflammatory cytokines, such as TNF-alpha, IL-6 and IL-1, indicating potential anti-inflammatory properties that warrant further investigation.

PROSPERO: CRD42023441699.

Clinical depression, including major depressive disorder (MDD), is a chronic mental illness characterized by persistent sadness and indifference. Depression is associated with neuroinflammation, oxidative stress, and neuronal apoptosis in the brain, resulting in microglial overactivation, decreased neuronal and glial proliferation, monoamine depletion, structural abnormalities, and aberrant biochemical activity via the hypothalamic-pituitary-adrenal axis. Recent studies have exhibited the role of dietary bioactive compounds in the mitigation of MDD progression. Here, in this narrative review, we reported the effects of commonly consumed bioactive compounds (curcumin, saffron, garlic, resveratrol, omega-3 fatty acids, ginger, blueberry, tea, and creatine) on MDD and MDD-related neuroinflammation and oxidative stress. The evidence reviewed here is almost exclusively from animal studies and strongly suggests that these commonly consumed bioactive compounds have anti-MDD effects as shown in antidepression-like behaviors, such as increased immobility, sucrose preference, and social interaction. On the basis of the literature/studies reviewed, the proposed molecular mechanisms include 1) the reduction of neuroinflammation activation and oxidative stress, 2) the enhancement of anti-inflammatory and antioxidant properties, 3) the reduction of monoamine oxidase-A production, and 4) the elevation of brain-derived neurotropic factor and neurogenesis. In the future, dietary bioactive compounds on clinical randomized controlled trials are warranted to confirm the findings of preclinical efficacies using bioactive compounds in individuals with MDD.

The demand for sustainable and eco-friendly alternatives to fossil and plant oil-derived chemicals has spurred interest in microbial production of lipids, particularly triacylglycerols, fatty acids, and their derivatives. Yeasts are promising platforms for synthesizing these compounds due to their high lipid accumulation capabilities, robust growth, and generally recognized as safe (GRAS) status. There is vast interest in fatty acid and triacylglycerol products with tailored fatty acid chain lengths and compositions, such as polyunsaturated fatty acids and substitutes for cocoa butter and palm oil. However, microbes naturally produce a limited set of mostly long-chain fatty acids, necessitating the development of microbial cell factories with customized fatty acid profiles. This review explores the capabilities of key enzymes involved in fatty acid and triacylglycerol synthesis, including fatty acid synthases, desaturases, elongases, and acyltransferases. It discusses factors influencing fatty acid composition and presents engineering strategies to enhance fatty acid synthesis. Specifically, we highlight successful engineering approaches to modify fatty acid profiles in triacylglycerols and produce tailored fatty acids, and we offer recommendations for host selection to streamline engineering efforts. KEY POINTS: • Detailed overview on all basic aspects of fatty acid metabolism in yeast • Comprehensive description of fatty acid profile tailoring in yeast • Extensive summary of applying tailored fatty acid profiles in production processes.

Heat stress (HS), driven by rising global temperatures, significantly impairs the nutritional composition and sensory quality of meat from monogastric animals, particularly swine and poultry. HS induces physiological disturbances, including reduced feed intake, oxidative stress, and endocrine disruption, which together reduce muscle protein content by 10-15% and essential amino acid levels (e.g., lysine, methionine, threonine) by 15-25%. Lipid profiles are also altered, with up to 30% reductions in polyunsaturated fatty acids (PUFAs), especially omega-3s, and an increased saturated fat content. Additionally, HS reduces the retention of vitamins E, A, D, and C by 20-50% and critical minerals such as selenium, zinc, and iron, compromising antioxidant capacity, immune function, and oxygen transport. These changes diminish meat tenderness, juiciness, flavour, and colour stability, leading to reduced consumer appeal and dietary quality. The consumption of heat-stressed meat may elevate risks for cardiovascular disease, oxidative stress, and micronutrient deficiencies. Mitigation strategies, including dietary antioxidant and osmolyte supplementation, genetic selection for thermotolerance, and optimised feeding practices, can reduce oxidative damage by up to 40% and improve nutrient retention. This review synthesises the current evidence on HS-induced meat quality deterioration and explores nutritional and management strategies to protect animal productivity and human health.

Oral mucositis (OM) is a serious complication of anticancer therapy that can substantially affect patient quality of life and treatment outcomes. This meta-analysis evaluated the efficacy of omega-3 fatty acids in the prevention and treatment of OM in patients diagnosed with cancer undergoing anticancer therapy. A systematic literature search of the PubMed, Embase, Web of Science, Cochrane Library, CNKI, and Wanfang databases for relevant studies, published up to September 24, 2024, was performed. Risk ratio (RR) or standardized mean difference (SMD) with corresponding 95% confidence interval (CIs) were calculated using Review Manager version 5.3. Five studies, including 337 patients, were included in this meta-analysis. Results of analysis revealed that, although omega-3 fatty acids did not significantly reduce the overall incidence of OM (RR 0.50, 95% CI 0.25-1.01), it significantly reduced the incidence of severe OM (RR 0.31, 95% CI 0.17-0.56), with no heterogeneity was observed (p = 0.96; I2= 0%). Furthermore, omega-3 fatty acids were found to significantly alleviate OM-associated pain (SMD -1.61, 95% CI -2.79 to -0.43), with no heterogeneity was detected (p = 0.32; I2 = 0%). Omega-3 fatty acids effectively reduced the incidence of severe OM and alleviated OM-related pain in patients undergoing anticancer therapy.

In recent years, the global incidence of chronic kidney disease (CKD) has been rising. As CKD progresses, it frequently involves inflammatory cell infiltration, contributing to renal fibrosis. Current research indicates that abnormalities in lipid metabolism play a role in this fibrotic process. However, the specific effects of various dietary fatty acids on renal inflammation and fibrosis remains largely unexplored. Our study demonstrates that dietary intake of omega-3 polyunsaturated fatty acids can inhibit macrophage activation and infiltration in a mouse model of unilateral ureteral obstruction (UUO), thus reducing the severity of renal fibrosis. Omega-3 polyunsaturated fatty acids, particularly α-linolenic acid (α-LA), mitigate damage to HK-2 cells and macrophages by targeting the JAG1-NOTCH1/2 pathway and by downregulating the expression of the chemokine MCP-1 and its receptor CCR2. This modulation attenuates macrophage activation and infiltration, reducing the inflammatory response. Furthermore, these fatty acids inhibit fibroblast chemotaxis, reduce fibroblast activation, and mitigate the deposition of extracellular matrix (ECM), thus slowing the progression of renal fibrosis. Our findings underscore the protective effects of omega-3 polyunsaturated fatty acids, such as α-LA, in preventing injury, inhibiting macrophage activation, and alleviating fibrosis. These results suggests that adjusting the dietary balance of fatty acids may offer a promising strategy to enhance the efficacy of CKD treatment.

Chronic obstructive pulmonary disease (COPD) is frequently accompanied by skeletal muscle dysfunction, a critical and severe extrapulmonary complication. This dysfunction contributes to reduced exercise capacity, increased frequency of acute exacerbations, and elevated mortality, serving as an independent risk factor for poor prognosis in COPD patients. Owing to the unique physicochemical conditions of the marine environment, marine-derived bioactive compounds exhibit potent anti-inflammatory and antioxidant properties, demonstrating therapeutic potential for ameliorating COPD skeletal muscle dysfunction. This review summarizes marine-derived bioactive compounds with promising efficacy against skeletal muscle dysfunction in COPD, including polysaccharides, lipids, polyphenols, peptides, and carotenoids. The discussed compounds have shown bioactivities in promoting skeletal muscle health and suppressing muscle atrophy, thereby providing potential strategies for the prevention and treatment of COPD skeletal muscle dysfunction. These findings may expand the therapeutic strategies for managing COPD skeletal muscle dysfunction.

Aging is associated with immune senescence and gut dysbiosis, both of which are heavily influenced by the diet. In this review, we summarize current knowledge regarding the impact of diets high in fiber, protein, or fat, as well as different dietary components (tryptophan, omega-3 fatty acids, and galacto-oligosaccharides) on the immune system and the gut microbiome in aging. Additionally, this review discusses how aging alters tryptophan metabolism, contributing to changes in immune function and the gut microbiome. Understanding the relationship between diet, the gut microbiome, and immune function in the context of aging is critical to formulate sound dietary recommendations for older individuals, and these personalized nutritional practices will ultimately improve the health and longevity of the elderly.

Eicosapentaenoic acid (EPA), and docosahexaenoic acid (DHA) are two biologically active omega-3 polyunsaturated fatty acids (n-3 PUFA), acquired by nutrition and incorporated in cell membranes' phospholipids, thus playing a crucial role in human health and homeostasis. Due to their potential cardioprotective, anti-inflammatory, and anti-arrhythmic actions, n-3 PUFA emerge as an interesting therapeutic option for cardiac surgery (CS) patients. The aim of this review was to assess the effects of perioperative administration of n-3 PUFA in CS patients. A comprehensive literature search was conducted in order to identify prospective cohort studies and randomized controlled trials (RCT) reporting on the perioperative effects of n-3 PUFA among adult patients undergoing CS. A total of 31 articles, published between 1995 and 2022, including 10 543 patients, met the inclusion criteria. There seems to be a beneficial effect of n-3 PUFA supplementation for arrhythmias such as in Postoperative Atrial Fibrillation (POAF), reduction of Intensive Care Unit Length of Stay (ICULOS) & Hospital Length of Stay (HLOS), reduction in postoperative ventilation time, in inotropic demand, in postoperative fatigue, as well as in overall morbidity and mortality. Moreover, n-3 PUFA increase antioxidant potential, attenuate oxidative stress and inflammation with subsequent significant reduction in myocardial ischemia/reperfusion (I/R) injury, thus promoting early metabolic recovery of the heart after elective CS leading to improved myocardial protection. They represent a readily available and cost-effective strategy that could improve the outcome of patients undergoing CS, by reducing the risks of serious cardiovascular adverse events (AE), both peri- and post-operatively.

Alzheimer's disease (AD), the most prevalent form of dementia, is a progressive neurodegenerative disorder characterized by a gradual decline in several domains of higher cortical function. Both preclinical and clinical research has suggested that the supplementation omega-3 fatty acids (FAs) may have potential benefits for individuals with AD. The present study aimed to identify all randomized controlled trials (RCTs) examining the association between omega-3 FA supplementation and cognitive function in patients with AD, using the Alzheimer's Disease Assessment Scale-Cognitive (ADAS-Cog) Subscale test as the primary outcome measure. A comprehensive search of the PubMed and Cochrane Library databases was conducted for all published RCTs up to December, 2023 that assessed cognition following omega-3 FA supplementation compared to placebo. A total of five studies met the eligibility criteria and were included in the qualitative synthesis, with four of these studies being incorporated into the meta-analysis. From these studies, data were collected from a total of 702 patients with AD, with 376 participants receiving omega-3 FA supplementation and 326 participants receiving a placebo. The primary outcome measure was the ADAS-Cog score. The meta-analysis revealed that omega-3 FA supplementation had a non-significant impact on the ADAS-Cog score compared to placebo, with a mean difference of 1.37 [95% confidence interval (CI) 0.00-2.73]. The heterogeneity among the included studies was moderate (I2=35%, P=0.17). The test for overall effect (z=1.96, P=0.05) indicated no statistical significance. Therefore, it was concluded that omega-3 FA supplementation does not significantly affect the cognitive function of adults with AD.

We performed a systematic review and meta-analysis to investigate the effects of combining omega-3 polyunsaturated fatty acids (n-3 PUFAs) supplementation with exercise training, as compared to exercise training alone, on body composition measures including body weight, body mass index (BMI), fat mass, body fat percentage, and lean body mass. Additionally, we determined the effects on cardiometabolic health outcomes including lipid profiles, blood pressure, glycemic markers, and inflammatory markers.

Three primary electronic databases including PubMed, Web of Science, and Scopus were searched from inception to April 5th, 2023 to identify original articles comparing n-3 PUFA supplementation plus exercise training versus exercise training alone, that investigated at least one of the following outcomes: fat mass, body fat percentage, lean body mass, triglycerides (TG), total cholesterol (TC), low-density lipoprotein (LDL), high-density lipoprotein (HDL), systolic (SBP) and diastolic (DBP) blood pressures, fasting glucose and insulin, interleukin-6 (IL-6), and tumor necrosis factor-alpha (TNF-α). Standardized mean differences (SMD) or weighted mean differences (WMD), and 95 % confidence intervals (CIs) were calculated using random-effects models.

A total of 21 studies involving 673 participants with BMIs ranging from 24 to 37 kg.m2 and ages ranging from 30 to 70 years were included in the meta-analysis. Overall, the results indicated that as compared with exercise training alone, adding omega-3 supplementation to exercise training decreased fat mass [WMD: -1.05 kg (95 % CI: -1.88 to -0.22), p = 0.01], TG [WMD: -0.10 mmol/L (95 % CI: -0.19 to -0.02)], SBP [WMD: -4.09 mmHg (95 % CI: -7.79 to -2.16), p = 0.03], DBP [WMD: -4.26 mmHg (95 % CI: -6.46 to -2.07), p = 0.001], and TNF-α [SMD: -0.35 (95 % CI: -0.70 to -0.00), p = 0.04], and increased LDL [WMD: 0.14 mmol/L (95 % CI: 0.02 to 0.26), p = 0.01] and lower-body muscular strength [SMD: 0.42 (95 % CI: 0.01 to 0.84), p = 0.04]. However, omega-3 supplementation with exercise training had no additional effects compared with training alone, for other body composition or cardiometabolic outcomes.

This systematic review and meta-analyses suggestes that adding omega-3 supplementation to exercise training may augment some effects of exercise training on body composition and cardiometabolic health in adults, although such effects appear to be modest.

Ischemic stroke (IS) is a cerebrovascular disease that predominantly affects middle-aged and elderly populations, exhibiting high mortality and disability rates. At present, the incidence of IS is increasing annually, with a notable trend towards younger affected individuals. Recent discoveries concerning the "gut-brain axis" have established a connection between the gut and the brain. Numerous studies have revealed that intestinal microbes play a crucial role in the onset, progression, and outcomes of IS. They are involved in the entire pathophysiological process of IS through mechanisms such as chronic inflammation, neural regulation, and metabolic processes. Although numerous studies have explored the relationship between IS and intestinal microbiota, comprehensive analyses of specific microbiota is relatively scarce. Therefore, this paper provides an overview of the typical changes in gut microbiota following IS and investigates the role of specific microorganisms in this context. Additionally, it presents a comprehensive analysis of post-stroke microbiological therapy and the relationship between IS and diet. The aim is to identify potential microbial targets for therapeutic intervention, as well as to highlight the benefits of microbiological therapies and the significance of dietary management. Overall, this paper seeks to provide key strategies for the treatment and management of IS, advocating for healthy diets and health programs for individuals. Meanwhile, it may offer a new perspective on the future interdisciplinary development of neurology, microbiology and nutrition.

Thirty male Hu lambs (38.95 ± 3.87 kg; 6 months old) were randomly assigned to two groups: (1) SBM (a basal diet with soybean meal) and (2) FSM (a diet replacing 10 % soybean meal with 10 % flax seed meal) to evaluate their effects on Hu lamb production and slaughter performance, meat quality, muscle fatty acid composition, and antioxidant capacity. The production and slaughter performance, as well as the amino acid composition in muscle were not different between groups. The FSM decreased muscle shear-force (P = 0.019), the pH 24h (P = 0.002), and the cooking loss (P = 0.008). The b* (yellowness, P = 0.039) and the a* (redness, P = 0.008) of the FSM were lower than those of the SBM. FSM group reduced muscle hardness (P = 0.004), gumminess (P = 0.009), chewiness (P = 0.007), and the diameter of muscle fibers (P < 0.05). A significant increase of total n-3 polyunsaturated fatty acids (P = 0.001), the content of α-linolenic acid (ALA, P = 0.003), homo-gamma-linolenic acid (P = 0.034), docosadienoic acid (P = 0.001), and docosahexaenoic acid (DHA, P = 0.003) was obtained in the FSM group. The CAT activity of FSM was significantly higher than that of SBM (P < 0.05). In conclusion, compared with the SBM, the FSM could effectively improve the meat tenderness, the content of ALA and DHA, and the catalase activity in muscle of Hu lambs.

Exercise-induced muscle damage (EIMD) refers to muscle injuries following exercises involving repetitive eccentric muscle contractions. The resultant inflammation and muscle protein leakage into the circulation lead to muscle pain and strength deficit, compromising athletic performance. This narrative review summarizes the current evidence on the effect and mechanism of omega-3 polyunsaturated fatty acids (n-3 PUFA) in potentially mitigating EIMD. Several studies suggested n-3 PUFA's role in alleviating delayed-onset muscle soreness, particularly in untrained individuals and those receiving higher doses of continuous supplementation. However, its impact on muscle strength attenuation and the reduction of performance post-exercise remains inconclusive. Also unclear are n-3 PUFA's effects on the reduction of circulating pro-inflammatory substances and muscle proteins. One of the possible mechanisms is its anti-inflammatory property, which involves its ability to incorporate into cell membranes and displace prostaglandin precursor. n-3 PUFA also decreases cyclooxygenase production and can be converted into specialized pro-resolving mediators (SPMs), further reducing inflammation. Moreover, n-3 PUFA's incorporation into cell membranes alters cell membrane properties, diminishing protein release during muscle breakdown. n-3 PUFA exhibits analgesic effects through SPM-induced modulation of receptors and ion channels, reducing both peripheral and central sensitization. n-3 PUFA also diminishes mitochondrial free radical production and accelerates nerve conduction, thereby improving voluntary muscle activation.

Nonalcoholic fatty liver disease (NAFLD) is a growing global health concern that is closely linked to metabolic syndrome, yet no approved pharmacological treatment exists. The Mediterranean diet (MD) emerged as a first-line dietary intervention for NAFLD, offering metabolic and hepatoprotective benefits. Now conceptualized as a complex chemical matrix rich in bioactive compounds, the MD exerts antioxidant and anti-inflammatory effects, improving insulin sensitivity and lipid metabolism. Mitochondria play a central role in NAFLD pathophysiology, influencing energy metabolism, oxidative stress, and lipid homeostasis. Emerging evidence suggests that the MD's bioactive compounds enhance mitochondrial function by modulating oxidative phosphorylation, biogenesis, and mitophagy. However, most research has focused on individual compounds rather than the MD as a whole, leaving gaps in understanding its collective impact as a complex dietary pattern. This narrative review explores how the MD and its bioactive compounds influence mitochondrial health in NAFLD, highlighting key pathways such as mitochondrial substrate control, dynamics, and energy efficiency. A literature search was conducted to identify relevant studies on the MD, mitochondria, and NAFLD. While the search was promising, our understanding remains incomplete, particularly when current knowledge is limited by the lack of mechanistic and comprehensive studies on the MD's holistic impact. Future research integrating cutting-edge experimental approaches is needed to elucidate the intricate diet-mitochondria interactions. A deeper understanding of how the MD influences mitochondrial health in NAFLD is essential for developing precision-targeted nutritional strategies that can effectively prevent and manage the disease.

The lipid compositions of wheat, triticale, rye, and tritordeum straws were thoroughly analyzed using gas chromatography-mass spectrometry (GC-MS). The major lipophilic compounds identified included n-fatty acids (1185-3538 mg/kg), β-diketones (891-2043 mg/kg), steroid compounds (1358-1954 mg/kg), high molecular-weight esters (444-1560 mg/kg), and n-fatty alcohols (402-1825 mg/kg). Additionally, smaller amounts of n-alkanes (140-574 mg/kg), phytol and phytyl esters (106-358 mg/kg), 2-hydroxyfatty acids (77-155 mg/kg), acylglycerides (41-277 mg/kg), tocopherols and tocopheryl esters (21-67 mg/kg), and n-aldehydes (10-23 mg/kg) were detected. The abundance and wide diversity of lipophilic compounds present in these agricultural residues highlight their great potential as a rich source of valuable phytochemicals for various industrial applications, positioning cereal straws as highly attractive feedstocks in the context of the lignocellulosic biorefinery.

Inflammatory bowel disease (IBD) is a relapsing disorder characterized by uncontrolled chronic inflammation of the gastrointestinal tract, posing a significant therapeutic challenge owing to the limited efficacy and undesirable side effects of current therapeutic options. A key pathological hallmark of IBD is the excessive production of reactive oxygen species (ROS). Hence, therapeutic strategies aimed at reducing ROS levels are promising for relieving these inflammatory conditions. Vitamin C-a natural nutrient for the human body-is well known for its potent antioxidant effects. However, the clinical development of vitamin C as a therapeutic drug has been hindered by its poor stability, rapid metabolism, and inadequate tissue accumulation. Herein, we report that the bioavailability of vitamin C can be enhanced by chemically reprogramming it with a small panel of long-chain fatty acids that aid in the aqueous self-assembly of the resulting drug conjugates to create self-deliverable nanoassemblies, enhancing their inflammation disease-oriented delivery and cellular uptake. In mice with dextran sulfate sodium-induced colitis, the optimal vitamin C-lipid nanoassemblies preferentially accumulated in inflamed colonic tissues following systemic administration and substantially ameliorated disease severity. We extended this strategy to incorporate the clinically approved glucocorticoid budesonide into the vitamin C nanosystem, facilitating a synergistic combination. In the chronic colitis model, the combination treatment reduced inflammation without compromising global immunity. Mechanistically, the treatment modulated the intestinal inflammatory microenvironment and altered the immune cell landscape, partly through regulation of the gut microbiome. Given its anticipated negligible side effects, this novel nanoassembly platform leveraging small-molecule lipidation may become a promising therapeutic for treating various inflammatory diseases.

Functional foods, thanks to their basic nutritional properties, can have physiological benefits and can alleviate the symptoms of many chronic diseases. They contain active components deriving either from plant or animal sources, and they show anti-inflammatory, cardiotonic, and antioxidant pharmacological activities that could be useful in preventing oxidative damage and inflammatory processes in a variety of disorders. There is evidence from in vitro, in vivo, and clinical observational studies that some compounds have significant effects in modulating the muscular dystrophy phenotype, which is characterized by fibrosis, myofiber necrotic cell death, inflammation, oxidative stress, and dysfunctional mitochondria. This review involves collecting data from the main medical databases and detailing the key features involved in muscular dystrophy progression and the relevance of fatty-acid compounds as diet supplements in the management of the disease. Omega fatty acids improve the dystrophic phenotype in terms of fibrosis and inflammation reduction, stimulating mitochondrial activity and antioxidant systems. Omega fatty acids could play a crucial role as food supplementation to delay dystrophy progression. This overview appears extremely relevant for researchers who are studying these molecules as valid alternatives to glucocorticoids, that today remain the only recognized pharmacological cure for dystrophic patients.

Previous epidemiological studies on the associations between polyunsaturated fatty acids (PUFAs) and cancer incidence have been inconsistent. We investigated the associations of plasma omega-3 and omega-6 PUFAs with the incidence of overall and 19 site-specific cancers in a large prospective cohort. 253,138 eligible UK Biobank participants were included in our study. With a mean follow-up of 12.9 years, 29,838 participants were diagnosed with cancer. The plasma levels of omega-3 and omega-6 PUFAs were expressed as percentages of total fatty acids (omega-3% and omega-6%). In our main models, both omega-6% and omega-3% were inversely associated with overall cancer incidence (HR per SD = 0.98, 95% CI = 0.96-0.99; HR per SD = 0.99, 95% CI = 0.97-1.00; respectively). Of the 19 site-specific cancers available, 14 were associated with omega-6% and five with omega-3%, all indicating inverse associations, with the exception that prostate cancer was positively associated with omega-3% (HR per SD = 1.03, 95% CI = 1.01-1.05). Our population-based cohort study in UK Biobank indicates small inverse associations of plasma omega-6 and omega-3 PUFAs with the incidence of overall and most site-specific cancers, although there are notable exceptions, such as prostate cancer.

Cancer is a disease influenced by both genetic and environmental factors, with dietary lipids being a significant contributing factor. This review summarizes the role of polyunsaturated fatty acids (PUFAs) in the mechanism of tumor occurrence and development, and elucidate the role of PUFAs in tumor treatment.

PUFAs exert their impact on cancer through altering lipid composition in cell membranes, interacting with cell membrane lipid receptors, directly modulating gene expression in the cell nucleus, and participating in the metabolism of lipid mediators. Most omega-3 PUFAs are believed to inhibit cell proliferation, promote cancer cell death, suppress cancer metastasis, alter energy metabolism, inhibit tumor microenvironment inflammation, and regulate immune responses involving macrophages, T cells, NK cells, and others. However, certain omega-6 PUFAs exhibit weaker anti-tumor effects and may even promote tumor development, such as by fostering inflammatory tumor microenvironment and enhancing tumor cell proliferation. PUFAs play important roles in hallmarks of cancer including tumor cell proliferation, cell death, migration and invasion, energy metabolism remodeling, epigenetics, and immunity. These findings provide insights into the mechanisms of cancer development and offers options for dietary management of cancer.

Stroke represents a predominant cause of mortality and disability on a global scale, impacting millions annually and exerting a considerable strain on healthcare systems. The incidence of stroke exhibits regional variability, with ischemic stroke accounting for the majority of occurrences. Post-stroke complications, such as cognitive impairment, motor dysfunction, and recurrent stroke, profoundly affect patients' quality of life. Recent advancements have elucidated the microbiota-gut-brain axis (MGBA), underscoring the complex interplay between gut health and brain function. Dysbiosis, characterized by an imbalance in gut microbiota, is significantly linked to an elevated risk of stroke and unfavorable outcomes. The MGBA plays a crucial role in modulating immune function, neurotransmitter levels, and metabolic byproducts, which may intensify neuroinflammation and impair cerebral health. This review elucidates the role of MGBA in stroke pathophysiology and explores potential gut-targeted therapeutic strategies to reduce stroke risk and promote recovery, including probiotics, prebiotics, pharmacological interventions, and dietary modifications. However, the current prevention and treatment strategies based on intestinal flora still face many problems, such as the large difference of individual intestinal flora, the stability of efficacy, and the long-term safety need to be considered. Further research needs to be strengthened to promote its better application in clinical practice.

Efforts to optimize the longevity of dairy cows are hindered by the increased risk of adverse health events, culling, or dying on farm with increased parity. Lipidomics provides a platform to help identify important biomarkers and biological pathways associated with increased parity and associated aging. A large multisite (15 pasture-based, 15 TMR farms) cross-sectional study collected plasma samples from nonlactating, late pregnant, dry cows (n = 696, ∼27 d prepartum) and peak milk cows (n = 796, ∼58 DIM) in a disproportionate stratified random sampling frame (parity: 0, 1, 2, >2 for dry cows; 1, 2, 3, >3 for peak milk cows). A total of 185 lipid species, comprising the lipids classes of phospholipids, sphingomyelins (SM) and triacylglycerols, were quantified in a targeted, liquid chromatography-MS approach. Dry and peak milk cohorts were analyzed separately throughout. Variation in lipid profiles were mostly attributed to farm of origin (36%-41% of variation), with feeding system explaining 13% to 21% and parity explaining 6% to 9%, according to ANOVA simultaneous component analysis modeling. Multiple linear regression and orthogonal partial least squares (O-PLS) investigated the association of the lipid profile with age (d), whereas discriminant analysis compared first parity with >3 parity cows in O-PLS discriminant analysis, random forest, and support vector machine models. Rankings of the most important lipid species for each model type were compared. Phospholipids with 40 carbon atoms and 6 double bond equivalents (40:6) were consistently decreased with increasing parity and age across both dry and peak milk cohorts. These lipids most likely contained stearate (18:0) and docosahexaenoic acid (DHA, C22:6n-3), an n-3 fatty acid. Additionally, phospholipids with 40:5 and 38:6, lysophosphatidylcholine (17:0), SM(35:1), and SM(35:2) were commonly identified lipids that decreased in concentration with parity and age. Docosahexaenoic acid has been associated with improved cattle health, reproduction, and milk production and quality. This study raises the hypothesis that reduced DHA levels in older cows may be an important factor increasing susceptibility to adverse health events, reduced reproductive performance, and herd removal. Studies that supplement DHA or its precursors can test this hypothesis and may be important in optimizing longevity of cows.