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Song SY, Lee SH, Bae MS, Park DH, Cho SS. Strong inhibition of xanthine oxidase and elastase of Baccharis trimera (Less.) DC stem extract and analysis of biologically active constituents. Front Pharmacol 2023; 14:1160330. [PMID: 37305531 PMCID: PMC10248149 DOI: 10.3389/fphar.2023.1160330] [Citation(s) in RCA: 3] [Impact Index Per Article: 1.5] [Reference Citation Analysis] [Abstract] [Key Words] [Grants] [Track Full Text] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 02/07/2023] [Accepted: 05/17/2023] [Indexed: 06/13/2023] Open
Abstract
Introduction: In the present study, strong xanthine oxidase and elastase activities of Baccharis trimera (Less) DC stem (BT) were evaluated and active ingredients were identified to determine the possibility of using BT extract as an anti-hyperuricemia (gout) and cosmetic functional material. Methods: Hot water, 20, 40, 60, 80, and 100% ethanolic extracts of BT were prepared. The hot water extract had the highest extraction yield whereas the 100% ethanolic extract had the lowest yield. Results and discussion: Antioxidant effects were investigated based on DPPH radical scavenging activity, reducing power, and total phenolic contents. The 80% ethanolic extract showed the highest antioxidant activity. However, the 100% ethanol BT extract showed strong xanthine oxidase and elastase inhibitory activities. Functional substances were thought to be caffeic acid and luteolin. Minor active substances such as o-coumaric acid, palmitic acid, naringenin, protocatechoic acid, and linoleic acid were identified. Through this study, we firstly reported evidence that BT stem extract could be used as functional materials with anti-hyperuricemia and skin disease improving effects. BT stem extract could be used as an anti-hyperuricemia (gout) natural drug or cosmetic material. For further study, practical studies such as optimizing BT extraction and functional experiments for hyperuricemia (gout) and skin wrinkle improvement are considered necessary.
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Affiliation(s)
- Seung-Yub Song
- Department of Pharmacy, College of Pharmacy, Mokpo National University, Muan, Jeonnam, Republic of Korea
- Department of Biomedicine, Health, and Life Convergence Sciences, BK21 Four, Biomedical and Healthcare Research Institute, Mokpo National University, Muan, Jeonnam, Republic of Korea
| | - Sung-Ho Lee
- Department of Pharmacy, College of Pharmacy, Mokpo National University, Muan, Jeonnam, Republic of Korea
- Department of Biomedicine, Health, and Life Convergence Sciences, BK21 Four, Biomedical and Healthcare Research Institute, Mokpo National University, Muan, Jeonnam, Republic of Korea
| | - Min-Suk Bae
- Department of Environmental Engineering, Mokpo National University, Muan, Jeonnam, Republic of Korea
| | - Dae-Hun Park
- College of Oriental Medicine, Dongshin University, Naju-si, Jeonnam, Republic of Korea
| | - Seung-Sik Cho
- Department of Pharmacy, College of Pharmacy, Mokpo National University, Muan, Jeonnam, Republic of Korea
- Department of Biomedicine, Health, and Life Convergence Sciences, BK21 Four, Biomedical and Healthcare Research Institute, Mokpo National University, Muan, Jeonnam, Republic of Korea
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Souza FRM, Silva GMM, Cadavid COM, Lisboa LDS, Silva MMCL, Paiva WS, Ferreira MJP, de Paula Oliveira R, Rocha HAO. Antioxidant Baccharis trimera Leaf Extract Suppresses Lipid Accumulation in C. elegans Dependent on Transcription Factor NHR-49. Antioxidants (Basel) 2022; 11:antiox11101913. [PMID: 36290635 PMCID: PMC9598929 DOI: 10.3390/antiox11101913] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 08/12/2022] [Revised: 09/12/2022] [Accepted: 09/15/2022] [Indexed: 11/16/2022] Open
Abstract
Obesity is a global public health problem that is associated with oxidative stress. One of the strategies for the treatment of obesity is the use of drugs; however, these are expensive and have numerous side effects. Therefore, the search for new alternatives is necessary. Baccharis trimera is used in Brazilian folk medicine for the treatment of obesity. Here, B. trimera leaf extract (BT) showed antioxidant activity in seven in vitro tests, and it was not toxic to 3T3 murine fibroblasts or Caenorhabditis elegans. Furthermore, BT reduces the intracellular amount of reactive oxygen species and increases C. elegans survival. Moreover, these effects were not dependent on transcription factors. The inhibition of fat accumulation by BT in the C. elegans model was also investigated. BT reduced lipid accumulation in animals fed diets without or with high amount of glucose. Furthermore, it was observed using RNA interference (iRNA) that BT depends on the transcription factor NHR-49 to exert its effect. Phytochemical analysis of BT revealed rutin, hyperoside, and 5-caffeoylquinic acid as the main BT components. Thus, these data demonstrate that BT has antioxidant and anti-obesity effects. However, further studies should be conducted to understand the mechanisms involved in its action.
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Affiliation(s)
- Flávia Roberta Monteiro Souza
- Laboratório de Biotecnologia de Polímeros Naturais (BIOPOL), Programa de Pós-graduação em Bioquímica e Biologia Molecular, Centro de Biociências, Federal University of Rio Grande do Norte—UFRN, Natal 59078-970, Brazil
| | - Giovanna Melo Martins Silva
- Laboratório de Genética Bioquímica (LGB), Programa de Pós-graduação em Biotecnologia, Centro de Biociências, Federal University of Rio Grande do Norte—UFRN, Natal 59078-970, Brazil
| | - Cesar Orlando Muñoz Cadavid
- Laboratório de Genética Bioquímica (LGB), Programa de Pós-graduação em Biotecnologia, Centro de Biociências, Federal University of Rio Grande do Norte—UFRN, Natal 59078-970, Brazil
| | - Lucas dos Santos Lisboa
- Laboratório de Biotecnologia de Polímeros Naturais (BIOPOL), Programa de Pós-graduação em Bioquímica e Biologia Molecular, Centro de Biociências, Federal University of Rio Grande do Norte—UFRN, Natal 59078-970, Brazil
| | - Maylla Maria Correia Leite Silva
- Laboratório de Biotecnologia de Polímeros Naturais (BIOPOL), Programa de Pós-graduação em Bioquímica e Biologia Molecular, Centro de Biociências, Federal University of Rio Grande do Norte—UFRN, Natal 59078-970, Brazil
| | - Weslley Souza Paiva
- Laboratório de Biotecnologia de Polímeros Naturais (BIOPOL), Programa de Pós-graduação em Bioquímica e Biologia Molecular, Centro de Biociências, Federal University of Rio Grande do Norte—UFRN, Natal 59078-970, Brazil
| | - Marcelo José Pena Ferreira
- Laboratório de Fitoquímica, Departamento de Botânica, Instituto de Biociências, Universidade de São Paulo—USP, Rua do Matão, 277, São Paulo 05508-090, Brazil
| | - Riva de Paula Oliveira
- Laboratório de Genética Bioquímica (LGB), Programa de Pós-graduação em Biotecnologia, Centro de Biociências, Federal University of Rio Grande do Norte—UFRN, Natal 59078-970, Brazil
| | - Hugo Alexandre Oliveira Rocha
- Laboratório de Biotecnologia de Polímeros Naturais (BIOPOL), Programa de Pós-graduação em Bioquímica e Biologia Molecular, Centro de Biociências, Federal University of Rio Grande do Norte—UFRN, Natal 59078-970, Brazil
- Correspondence: ; Tel.: +55-84-99999-9561
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Antunes C, Arbo MD, Konrath EL. Hepatoprotective Native Plants Documented in Brazilian Traditional Medicine Literature: Current Knowledge and Prospects. Chem Biodivers 2022; 19:e202100933. [PMID: 35421282 DOI: 10.1002/cbdv.202100933] [Citation(s) in RCA: 8] [Impact Index Per Article: 2.7] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 11/26/2021] [Accepted: 04/14/2022] [Indexed: 11/11/2022]
Abstract
The native flora of Brazil covers a large biodiversity of plants, some of which have important pharmacological properties. Ethnobotanical studies have established that the folk use of plants for hepatobiliary disorders is very common in Brazil. This review aimed to compile, describe and discuss the main native Brazilian medicinal plants used as hepatoprotective agents listed on 25 books by authors from various regions in the country. Based on the 153 species found, a comprehensive analysis of the most relevant scientific studies is presented, including Piper umbellatum, Echinodorus macrophyllus, Fevillea trilobata and Achyrocline satureioides. Some medicinal plants share both a large number of citations and scientific evidence to corroborate their hepatoprotective effects, including Solanum paniculatum, Baccharis crispa and Phyllanthus niruri, which could be of interest to develop new phytomedicines. Additionally, some active ingredients are indicated as being responsible for the liver protective effects described, particularly phenolic compounds (flavonoids, coumestans and lignans).
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Affiliation(s)
- Carolina Antunes
- Faculdade de Farmácia, Programa de Pós-Graduação em Ciências Farmacêuticas, Universidade Federal do Rio Grande do Sul (UFRGS), 90610-000, Porto Alegre-RS, Brazil
| | - Marcelo Dutra Arbo
- Faculdade de Farmácia, Programa de Pós-Graduação em Ciências Farmacêuticas, Universidade Federal do Rio Grande do Sul (UFRGS), 90610-000, Porto Alegre-RS, Brazil
| | - Eduardo Luis Konrath
- Faculdade de Farmácia, Programa de Pós-Graduação em Ciências Farmacêuticas, Universidade Federal do Rio Grande do Sul (UFRGS), 90610-000, Porto Alegre-RS, Brazil
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Support vector regression-based QSAR models for prediction of antioxidant activity of phenolic compounds. Sci Rep 2021; 11:8806. [PMID: 33888843 PMCID: PMC8062522 DOI: 10.1038/s41598-021-88341-1] [Citation(s) in RCA: 18] [Impact Index Per Article: 4.5] [Reference Citation Analysis] [Abstract] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 03/02/2021] [Accepted: 04/12/2021] [Indexed: 12/15/2022] Open
Abstract
The Support vector regression (SVR) was used to investigate quantitative structure-activity relationships (QSAR) of 75 phenolic compounds with Trolox-equivalent antioxidant capacity (TEAC). Geometric structures were optimized at the EF level of the MOPAC software program. Using Pearson correlation coefficient analysis, four molecular descriptors [n(OH), Cosmo Area (CA), Core-Core Repulsion (CCR) and Final Heat of Formation (FHF)] were selected as independent variables. The QSAR model was developed from the training set consisting of 57 compounds and then used the leave-one-out cross-validation (LOOCV) correlation coefficient to evaluate the prediction ability of the QSAR model. Used Artificial neural network (ANN) and multiple linear regression (MLR) for comparing. The RMSE (root mean square error) values of LOOCV in SVR, ANN and MLR models were 0.44, 0.46 and 0.54. The RMSE values of prediction of external 18 compounds were 0.41, 0.39 and 0.54 for SVR, ANN and MLR models, respectively. The obtained result indicated that the SVR models exhibited excellent predicting performance and competent for predicting the TEAC of phenolic compounds.
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Chaves PFP, Adami ER, Acco A, Iacomini M, Cordeiro LMC. Chemical characterization of polysaccharides from Baccharis trimera (Less.) DC. infusion and its hepatoprotective effects. Food Res Int 2020; 136:109510. [PMID: 32846588 DOI: 10.1016/j.foodres.2020.109510] [Citation(s) in RCA: 4] [Impact Index Per Article: 0.8] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 02/26/2020] [Revised: 06/25/2020] [Accepted: 06/28/2020] [Indexed: 12/13/2022]
Abstract
Baccharis trimera is a native medicinal plant from South America popularly known as "carqueja". Its infusion is traditionally ingested for the treatment and prevention of hepatic disorders. Up to now, only the crude aqueous extract or hydroethanolic fractions, containing the secondary metabolites, have been studied and correlated with their biological action on the liver. Here we report that an inulin type fructan is present in the B. trimera infusion and contributes to the hepatoprotective effect of the species. In vitro, inulin at 300 μg/mL, was able to scavenger 97% of the DPPH radicals. In vivo experiments showed that it protected the liver against CCl4-induced injuries. The administration of inulin at low dose of 1 mg/kg significantly reduced the blood levels of ALT, AST and ALP, reduced the lipid peroxidation and increased the catalase activity and the levels of reduced glutathione in the liver of CCl4-treated mice. Moreover, the administration of inulin at 100 mg/kg increased GSH levels in the liver of Naïve mice. No signs of toxicity were observed. Thus, inulin present in B. trimera infusion protects the liver from the oxidative stress caused by CCl4 administration and can corroborate with the hepatoprotective effects presented by the species infusion.
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Affiliation(s)
- Pedro Felipe Pereira Chaves
- Biochemistry and Molecular Biology Department, Federal University of Paraná, CP 19.046, CEP 81.531-980 Curitiba, PR, Brazil
| | - Eliana Rezende Adami
- Pharmacology Department, Federal University of Paraná, CEP 81.531-980 Curitiba, PR, Brazil
| | - Alexandra Acco
- Pharmacology Department, Federal University of Paraná, CEP 81.531-980 Curitiba, PR, Brazil
| | - Marcello Iacomini
- Biochemistry and Molecular Biology Department, Federal University of Paraná, CP 19.046, CEP 81.531-980 Curitiba, PR, Brazil
| | - Lucimara Mach Côrtes Cordeiro
- Biochemistry and Molecular Biology Department, Federal University of Paraná, CP 19.046, CEP 81.531-980 Curitiba, PR, Brazil.
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Barbosa RJ, Ratti da Silva G, Cola IM, Kuchler JC, Coelho N, Barboza LN, Menetrier JV, de Souza R, Zonta FN, Froehlich DL, Jacomassi E, Soares AA, Velasques LG, Veiga ADA, Souza LMD, Lovato ECW, Ribeiro-Paes JT, Gasparotto Junior A, Acco A, Lívero FADR. Promising therapeutic use of Baccharis trimera (less.) DC. as a natural hepatoprotective agent against hepatic lesions that are caused by multiple risk factors. JOURNAL OF ETHNOPHARMACOLOGY 2020; 254:112729. [PMID: 32145332 DOI: 10.1016/j.jep.2020.112729] [Citation(s) in RCA: 15] [Impact Index Per Article: 3.0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Subscribe] [Scholar Register] [Received: 10/08/2019] [Revised: 02/03/2020] [Accepted: 02/29/2020] [Indexed: 06/10/2023]
Abstract
ETHNOPHARMACOLOGICAL RELEVANCE Baccharis trimera (Less.) DC is a perennial subshrub, popularly known as "carqueja," that belongs to the Asteraceae family. Ethnobotanical studies indicate that this species is used for the treatment of diabetes and digestive and liver diseases. However, studies that sought to validate its popular use were conducted using ethanolic extracts of the plant, which does not reflect the ethnomedicinal use of this species in humans. AIM OF THE STUDY Non-alcoholic fatty liver disease (NAFLD) is characterized by triglyceride accumulation in the liver that can progress to cirrhosis and hepatocellular carcinoma. Because of the severity of this disease, less toxic and more effective therapeutic agents need to be developed. B. trimera may be a promising therapeutic alternative, but its activity against multiple risk factors for liver disease (e.g., smoking, dyslipidemia, and diabetes mellitus) has not been studied. The present study investigated the effects of an ethnomedicinal form of a B. trimera preparation in a rat model of NAFLD that is associated with multiple risk factors. MATERIAL AND METHODS Phytochemical analysis of the ethanolic soluble fraction of B. trimera extract was performed using ultra-performance liquid chromatography coupled to high-resolution mass spectrometry. Streptozotocin was used to induce diabetes in male Wistar rats. The rats received a 0.5% cholesterol-enriched diet and were exposed to cigarette smoke (9 cigarettes/day, 5 days/week, for 4 weeks). In the last 2 weeks, the animals were orally treated with vehicle (negative control group), B. trimera extract (30, 100, and 300 mg/kg), or insulin + simvastatin. One group of rats that was not exposed to these risk factors was also evaluated. Blood was collected for glucose, alanine aminotransferase (ALT), and aspartate aminotransferase (AST) analysis. The liver and feces were collected for lipid quantification. The liver was additionally processed for histopathological analysis. RESULTS The model successfully induced NAFLD and increased levels of glucose, AST, and ALT in the negative control group. Treatment with the B. trimera extract (30 and 100 mg/kg) and insulin + simvastatin decreased hepatic and fecal lipids. In contrast to insulin + simvastatin treatment, all three doses of B. trimera effectively reduced AST and ALT levels. CONCLUSION B. trimera may be promising as a hepatoprotective agent against hepatic lesions that are caused by multiple risk factors.
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Affiliation(s)
- Rodrigo Jachimowski Barbosa
- Laboratory of Preclinical Research of Natural Products, Post-Graduate Program in Medicinal Plants and Phytotherapeutics in Basic Attention, Paranaense University, Umuarama, PR, Brazil
| | - Gustavo Ratti da Silva
- Laboratory of Preclinical Research of Natural Products, Post-Graduate Program in Medicinal Plants and Phytotherapeutics in Basic Attention, Paranaense University, Umuarama, PR, Brazil
| | - Itaruã Machri Cola
- Laboratory of Preclinical Research of Natural Products, Post-Graduate Program in Medicinal Plants and Phytotherapeutics in Basic Attention, Paranaense University, Umuarama, PR, Brazil
| | | | - Natalia Coelho
- Laboratory of Preclinical Research of Natural Products, Post-Graduate Program in Medicinal Plants and Phytotherapeutics in Basic Attention, Paranaense University, Umuarama, PR, Brazil
| | - Lorena Neris Barboza
- Laboratory of Preclinical Research of Natural Products, Post-Graduate Program in Medicinal Plants and Phytotherapeutics in Basic Attention, Paranaense University, Umuarama, PR, Brazil
| | - Jacqueline Vergutz Menetrier
- Laboratory of Preclinical Research of Natural Products, Post-Graduate Program in Medicinal Plants and Phytotherapeutics in Basic Attention, Paranaense University, Umuarama, PR, Brazil
| | - Ronaldo de Souza
- Laboratory of Preclinical Research of Natural Products, Post-Graduate Program in Medicinal Plants and Phytotherapeutics in Basic Attention, Paranaense University, Umuarama, PR, Brazil
| | | | | | - Ezilda Jacomassi
- Laboratory of Preclinical Research of Natural Products, Post-Graduate Program in Medicinal Plants and Phytotherapeutics in Basic Attention, Paranaense University, Umuarama, PR, Brazil
| | - Andréia Assunção Soares
- Laboratory of Preclinical Research of Natural Products, Post-Graduate Program in Medicinal Plants and Phytotherapeutics in Basic Attention, Paranaense University, Umuarama, PR, Brazil
| | - Leonardo Garcia Velasques
- Laboratory of Preclinical Research of Natural Products, Post-Graduate Program in Medicinal Plants and Phytotherapeutics in Basic Attention, Paranaense University, Umuarama, PR, Brazil
| | - Alan de Almeida Veiga
- Institute of Research Pelé Pequeno Príncipe, Pequeno Príncipe Faculty, Curitiba, PR, Brazil
| | - Lauro Mera de Souza
- Institute of Research Pelé Pequeno Príncipe, Pequeno Príncipe Faculty, Curitiba, PR, Brazil
| | - Evellyn Claudia Wietzkoski Lovato
- Laboratory of Preclinical Research of Natural Products, Post-Graduate Program in Medicinal Plants and Phytotherapeutics in Basic Attention, Paranaense University, Umuarama, PR, Brazil
| | | | - Arquimedes Gasparotto Junior
- Laboratory of Electrophysiology and Cardiovascular Pharmacology, Faculty of Health Sciences, Federal University of Grande Dourados, Dourados, MS, Brazil.
| | - Alexandra Acco
- Laboratory of Pharmacology and Metabolism, Post-Graduate Program of Pharmacology, Federal University of Paraná, Curitiba, PR, Brazil
| | - Francislaine Aparecida Dos Reis Lívero
- Laboratory of Preclinical Research of Natural Products, Post-Graduate Program in Medicinal Plants and Phytotherapeutics in Basic Attention, Paranaense University, Umuarama, PR, Brazil.
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Chemical characterization, antihyperlipidaemic and antihyperglycemic effects of Brazilian bitter quina species in mice consuming a high-refined carbohydrate diet. J Funct Foods 2019. [DOI: 10.1016/j.jff.2019.01.030] [Citation(s) in RCA: 7] [Impact Index Per Article: 1.2] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 01/09/2023] Open
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Biotoxicological Analyses of Trimeroside from Baccharis trimera Using a Battery of In Vitro Test Systems. OXIDATIVE MEDICINE AND CELLULAR LONGEVITY 2018; 2018:7804135. [PMID: 30210656 PMCID: PMC6120265 DOI: 10.1155/2018/7804135] [Citation(s) in RCA: 3] [Impact Index Per Article: 0.4] [Reference Citation Analysis] [Abstract] [Track Full Text] [Download PDF] [Figures] [Subscribe] [Scholar Register] [Received: 11/22/2017] [Revised: 02/24/2018] [Accepted: 07/11/2018] [Indexed: 11/24/2022]
Abstract
The use in folk medicine of Baccharis trimera and recent studies on DNA damage by oxidative stress mechanisms have motivated this study. We investigated the biotoxicological effects of trimeroside from this plant. Aqueous extract from aerial parts of B. trimera was fractioned by flash chromatography for further isolation by thin-layer chromatography. The novel nor-monoterpene glycoside, trimeroside, and three flavonoids, cirsimaritin, luteolin and quercetin, were isolated. The genotoxic and mutagenic potential of trimeroside was determined by Salmonella/microsome (TA98 and TA100), comet assay, and cytokinesis-block micronucleus cytome assay (CBMN-cyt) in HepG2 cells. We also screened trimeroside into different human tumoral cell lines by sulforhodamine B (SRB) assay. Mutagenicity was detected in TA100 strain with metabolic activation. Genotoxic effects were not observed in HepG2 by comet assay. However, a decrease in the nuclear index division in the 2.0 mg·mL−1 concentration and an increase of nucleoplasmic bridges in the 1.5 mg·mL−1 concentration were detected by CBMN-cyt assay indicating cytotoxic and mutagenic effects. In SRB assay, trimeroside showed weak antiproliferative activity against the cell lines.
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Martínez ML, Bettucci GR, Ferretti MD, Campagna MN, Ansaldi N, Cortadi AA, Rodriguez MV. Multivariate statistical analysis of morpho-anatomical data of nine sect. Caulopterae species ( Baccharis – Asteraceae) used in folk medicine. REVISTA BRASILEIRA DE FARMACOGNOSIA 2018. [DOI: 10.1016/j.bjp.2018.05.002] [Citation(s) in RCA: 2] [Impact Index Per Article: 0.3] [Reference Citation Analysis] [Track Full Text] [Subscribe] [Scholar Register] [Indexed: 11/27/2022]
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Rabelo ACS, de Pádua Lúcio K, Araújo CM, de Araújo GR, de Amorim Miranda PH, Carneiro ACA, de Castro Ribeiro ÉM, de Melo Silva B, de Lima WG, Costa DC. Baccharis trimera protects against ethanol induced hepatotoxicity in vitro and in vivo. JOURNAL OF ETHNOPHARMACOLOGY 2018; 215:1-13. [PMID: 29289796 DOI: 10.1016/j.jep.2017.12.043] [Citation(s) in RCA: 16] [Impact Index Per Article: 2.3] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Subscribe] [Scholar Register] [Received: 01/03/2017] [Revised: 12/13/2017] [Accepted: 12/26/2017] [Indexed: 06/07/2023]
Abstract
ETHNOPHARMACOLOGICAL RELEVANCE Baccharis trimera has been traditionally used in Brazil to treat liver diseases. AIM OF THE STUDY To evaluate the protective effect of Baccharis trimera in an ethanol induced hepatotoxicity model. MATERIALS AND METHODS The antioxidant capacity was evaluated in vitro by the ability to scavenged the DPPH radical, by the quantification of ROS, NO and the transcription factor Nrf2. Hepatotoxicity was induced in animals by administration of absolute ethanol for 2 days (acute) or with ethanol diluted for 28 days (chronic). The biochemical parameters of hepatic function (ALT and AST), renal function (urea and creatinine) and lipid profile (total cholesterol, triglycerides and HDL) were evaluated. In addition to antioxidant defense (SOD, catalase, glutathione), oxidative damage markers (TBARS and carbonylated protein), MMP-2 activity and liver histology. RESULTS Baccharis trimera promoted a decrease in ROS and NO, and at low concentrations promoted increased transcription of Nrf2. In the acute experiment it promoted increase of HDL, in the activity of SOD and GPx, besides diminishing TBARS and microesteatosis. Already in the chronic experiment B. trimera improved the hepatic and renal profile, decreased triglycerides and MMP-2 activity, in addition to diminishing microesteatosis. CONCLUSION We believe that B. trimera action is possibly more associated with direct neutralizing effects or inhibition of reactive species production pathways rather than the modulation of the antioxidant enzymes activity. Thus it is possible to infer that the biological effects triggered by adaptive responses are complex and multifactorial depending on the dose, the time and the compounds used.
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Affiliation(s)
- Ana Carolina Silveira Rabelo
- Laboratory of Metabolic Biochemistry (LBM), Department of Biological Sciences (DECBI), Center of Research in Biological Sciences (NUPEB), Federal University of Ouro Preto (UFOP), Ouro Preto, MG 35400-000, Brazil
| | - Karine de Pádua Lúcio
- Laboratory of Metabolic Biochemistry (LBM), Department of Biological Sciences (DECBI), Center of Research in Biological Sciences (NUPEB), Federal University of Ouro Preto (UFOP), Ouro Preto, MG 35400-000, Brazil
| | - Carolina Morais Araújo
- Laboratory of Metabolic Biochemistry (LBM), Department of Biological Sciences (DECBI), Center of Research in Biological Sciences (NUPEB), Federal University of Ouro Preto (UFOP), Ouro Preto, MG 35400-000, Brazil
| | - Glaucy Rodrigues de Araújo
- Laboratory of Metabolic Biochemistry (LBM), Department of Biological Sciences (DECBI), Center of Research in Biological Sciences (NUPEB), Federal University of Ouro Preto (UFOP), Ouro Preto, MG 35400-000, Brazil
| | - Pedro Henrique de Amorim Miranda
- Laboratory of Metabolic Biochemistry (LBM), Department of Biological Sciences (DECBI), Center of Research in Biological Sciences (NUPEB), Federal University of Ouro Preto (UFOP), Ouro Preto, MG 35400-000, Brazil
| | - Ana Claudia Alvarenga Carneiro
- Laboratory of Biology and Biotechnology of Microorganisms, Department of Biological Sciences (DECBI), Center of Research in Biological Sciences (NUPEB), Federal University of Ouro Preto (UFOP), Ouro Preto, MG 35400-000, Brazil
| | - Érica Milena de Castro Ribeiro
- Laboratory of Biology and Biotechnology of Microorganisms, Department of Biological Sciences (DECBI), Center of Research in Biological Sciences (NUPEB), Federal University of Ouro Preto (UFOP), Ouro Preto, MG 35400-000, Brazil
| | - Breno de Melo Silva
- Laboratory of Biology and Biotechnology of Microorganisms, Department of Biological Sciences (DECBI), Center of Research in Biological Sciences (NUPEB), Federal University of Ouro Preto (UFOP), Ouro Preto, MG 35400-000, Brazil
| | - Wanderson Geraldo de Lima
- Laboratory of Morphopathology (LMP), Department of Biological Sciences (DECBI), Center of Research in Biological Sciences (NUPEB), Federal University of Ouro Preto (UFOP), Ouro Preto, MG 35400-000, Brazil
| | - Daniela Caldeira Costa
- Laboratory of Metabolic Biochemistry (LBM), Department of Biological Sciences (DECBI), Center of Research in Biological Sciences (NUPEB), Federal University of Ouro Preto (UFOP), Ouro Preto, MG 35400-000, Brazil.
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Balaban YH, Aka C, Koca-Caliskan U. Liver immunology and herbal treatment. World J Hepatol 2017; 9:757-770. [PMID: 28660010 PMCID: PMC5474722 DOI: 10.4254/wjh.v9.i17.757] [Citation(s) in RCA: 8] [Impact Index Per Article: 1.0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Journal Information] [Submit a Manuscript] [Subscribe] [Scholar Register] [Received: 03/04/2017] [Revised: 04/29/2017] [Accepted: 05/18/2017] [Indexed: 02/06/2023] Open
Abstract
Beyond the metabolic functions, the liver recently has been defined as an organ of immune system (IS), which have central regulatory role for innate and adaptive immunity. The liver keeps a delicate balance between hepatic screening of pathogenic antigens and immune tolerance to self-antigens. Herbal treatments with immunological effects have potential to alter this hepatic immune balance towards either therapeutic side or diseases side by inducing liver injury via hepatotoxicity or initiation of autoimmune diseases. Most commonly known herbal treatments, which have therapeutic effect on liver and IS, have proven via in vitro, in vivo, and/or clinical studies were summarized in this review.
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Affiliation(s)
- Yasemin H Balaban
- Yasemin H Balaban, Gastroenterology Unit, Private Etimed Hospital, 06790 Ankara, Turkey
| | - Ceylan Aka
- Yasemin H Balaban, Gastroenterology Unit, Private Etimed Hospital, 06790 Ankara, Turkey
| | - Ufuk Koca-Caliskan
- Yasemin H Balaban, Gastroenterology Unit, Private Etimed Hospital, 06790 Ankara, Turkey
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Baccharis trimera (Less.) DC Exhibits an Anti-Adipogenic Effect by Inhibiting the Expression of Proteins Involved in Adipocyte Differentiation. Molecules 2017; 22:molecules22060972. [PMID: 28604636 PMCID: PMC6152623 DOI: 10.3390/molecules22060972] [Citation(s) in RCA: 13] [Impact Index Per Article: 1.6] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 03/17/2017] [Revised: 05/20/2017] [Accepted: 06/07/2017] [Indexed: 01/12/2023] Open
Abstract
Baccharis trimera (Less.) DC (gorse) is a plant popularly used for the treatment of obesity. In this study, we prepared three B. trimera extracts aqueous extract (AE), decoction (AE-D), and methanol extract (ME) and investigated their antioxidant effects in six different tests and their anti-adipogenic effect in 3T3-L1 cells. The extracts showed a dose-dependent antioxidant activity in all tests. AE was the most potent antioxidant in copper and ferric ion chelation assays, whereas AE-D was the most potent in superoxide and hydroxyl radical scavenging assays, reducing power assay, and total antioxidant capacity analysis. Only ME showed a cytotoxic effect against 3T3-L1 cells. Lipid accumulation decreased in 3T3-L1 adipocytes in the presence of AE and AE-D extracts (0.5 to 1.0 mg/mL). In addition, the extracts dramatically attenuated the levels of adipogenic transcriptional factors, including CCAAT enhancer-binding protein α (C/EBPα), CCAAT enhancer-binding protein β (C/EBPβ), and gamma receptors by peroxisome proliferators (PPARγ), during adipogenesis. AE-D (1.0 mg/mL) caused an approximately 90% reduction in the levels of these molecules. We propose that B. trimera has an anti-adipogenic effect and could be used in the development of functional foods.
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14
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Hydroethanolic extract of Baccharis trimera ameliorates alcoholic fatty liver disease in mice. Chem Biol Interact 2016; 260:22-32. [DOI: 10.1016/j.cbi.2016.10.003] [Citation(s) in RCA: 25] [Impact Index Per Article: 2.8] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 07/20/2016] [Revised: 09/09/2016] [Accepted: 10/03/2016] [Indexed: 12/20/2022]
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15
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Biological Evaluation of Terrestrial and Marine Plant Originated Labdane Diterpenes (A Review). Pharm Chem J 2016. [DOI: 10.1007/s11094-016-1490-2] [Citation(s) in RCA: 12] [Impact Index Per Article: 1.3] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 12/20/2022]
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16
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de Araújo GR, Rabelo ACS, Meira JS, Rossoni-Júnior JV, Castro-Borges WD, Guerra-Sá R, Batista MA, Silveira-Lemos DD, Souza GHBD, Brandão GC, Chaves MM, Costa DC. Baccharis trimera inhibits reactive oxygen species production through PKC and down-regulation p47 phox phosphorylation of NADPH oxidase in SK Hep-1 cells. Exp Biol Med (Maywood) 2016; 242:333-343. [PMID: 28103717 DOI: 10.1177/1535370216672749] [Citation(s) in RCA: 9] [Impact Index Per Article: 1.0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 12/22/2022] Open
Abstract
Baccharis trimera, popularly known as "carqueja", is a native South-American plant possessing a high concentration of polyphenolic compounds and therefore high antioxidant potential. Despite the antioxidant potential described for B. trimera, there are no reports concerning the signaling pathways involved in this process. So, the aim of the present study was to assess the influence of B. trimera on the modulation of PKC signaling pathway and to characterize the effect of the nicotinamide adenine dinucleotide phosphate oxidase enzyme (NOX) on the generation of reactive oxygen species in SK Hep-1 cells. SK-Hep 1 cells were treated with B. trimera, quercetin, or rutin and then stimulated or not with PMA/ionomycin and labeled with carboxy H2DCFDA for detection of reactive oxygen species by flow cytometer. The PKC expression by Western blot and enzyme activity was performed to evaluate the influence of B. trimera and quercetin on PKC signaling pathway. p47 phox and p47 phox phosphorylated expression was performed by Western blot to evaluate the influence of B. trimera on p47 phox phosphorylation. The results showed that cells stimulated with PMA/ionomycin (activators of PKC) showed significantly increased reactive oxygen species production, and this production returned to baseline levels after treatment with DPI (NOX inhibitor). Both B. trimera and quercetin modulated reactive oxygen species production through the inhibition of PKC protein expression and enzymatic activity, also with inhibition of p47 phox phosphorylation. Taken together, these results suggest that B. trimera has a potential mechanism for inhibiting reactive oxygen species production through the PKC signaling pathway and inhibition subunit p47 phox phosphorylation of nicotinamide adenine dinucleotide phosphate oxidase.
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Affiliation(s)
- Glaucy Rodrigues de Araújo
- 1 Center for Research in Biological Sciences - NUPEB, Federal University of Ouro Preto, 35400-000 Ouro Preto, Brazil
| | | | - Janaína Serenato Meira
- 2 Department of Biological Sciences, Federal University of Ouro Preto, 35400-000 Ouro Preto, Brazil
| | - Joamyr Victor Rossoni-Júnior
- 1 Center for Research in Biological Sciences - NUPEB, Federal University of Ouro Preto, 35400-000 Ouro Preto, Brazil
| | - William de Castro-Borges
- 1 Center for Research in Biological Sciences - NUPEB, Federal University of Ouro Preto, 35400-000 Ouro Preto, Brazil.,2 Department of Biological Sciences, Federal University of Ouro Preto, 35400-000 Ouro Preto, Brazil
| | - Renata Guerra-Sá
- 1 Center for Research in Biological Sciences - NUPEB, Federal University of Ouro Preto, 35400-000 Ouro Preto, Brazil.,2 Department of Biological Sciences, Federal University of Ouro Preto, 35400-000 Ouro Preto, Brazil
| | - Maurício Azevedo Batista
- 3 Postgraduate Program in Parasitology, Immunology Laboratory and Genomic Parasites, Federal University of Minas Gerais, 31270-901 Belo Horizonte, MG, Brazil
| | - Denise da Silveira-Lemos
- 3 Postgraduate Program in Parasitology, Immunology Laboratory and Genomic Parasites, Federal University of Minas Gerais, 31270-901 Belo Horizonte, MG, Brazil
| | - Gustavo Henrique Bianco de Souza
- 1 Center for Research in Biological Sciences - NUPEB, Federal University of Ouro Preto, 35400-000 Ouro Preto, Brazil.,4 Department of Pharmaceutical Sciences - DEFAR, Program Postgraduate Pharmaceutical Sciences (CIPHARMA), School of Pharmacy, Federal University of Ouro Preto, 35400-000 Ouro Preto, Brazil
| | - Geraldo Célio Brandão
- 4 Department of Pharmaceutical Sciences - DEFAR, Program Postgraduate Pharmaceutical Sciences (CIPHARMA), School of Pharmacy, Federal University of Ouro Preto, 35400-000 Ouro Preto, Brazil
| | - Míriam Martins Chaves
- 5 Department of Biochemistry and Immunology, Federal University of Minas Gerais, 31270-901 Belo Horizonte, Brazil
| | - Daniela Caldeira Costa
- 1 Center for Research in Biological Sciences - NUPEB, Federal University of Ouro Preto, 35400-000 Ouro Preto, Brazil.,2 Department of Biological Sciences, Federal University of Ouro Preto, 35400-000 Ouro Preto, Brazil
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Gogula T, Yerrabelly JR. Synthesis of Diverse Oxa-Carbocycle-Annulated Flavones Using the CombinedClaisenRearrangement and Ring-Closing Metathesis. Helv Chim Acta 2016. [DOI: 10.1002/hlca.201600028] [Citation(s) in RCA: 8] [Impact Index Per Article: 0.9] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 11/06/2022]
Affiliation(s)
- Thirupathi Gogula
- Department of Chemistry; Osmania University; Hyderabad Telangana 500007 India
| | - Jayaprakash Rao Yerrabelly
- Department of Chemistry; Osmania University; Hyderabad Telangana 500007 India
- Department of Chemistry; Telangana University; Nizamabad 503322 India
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18
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Menezes APS, da Silva J, Fisher C, da Silva FR, Reyes JM, Picada JN, Ferraz AG, Corrêa DS, Premoli SM, Dias JF, de Souza CT, Ferraz ADBF. Chemical and toxicological effects of medicinal Baccharis trimera extract from coal burning area. CHEMOSPHERE 2016; 146:396-404. [PMID: 26741544 DOI: 10.1016/j.chemosphere.2015.12.028] [Citation(s) in RCA: 9] [Impact Index Per Article: 1.0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Subscribe] [Scholar Register] [Received: 10/08/2015] [Revised: 12/01/2015] [Accepted: 12/08/2015] [Indexed: 06/05/2023]
Abstract
The entire process of power generation, extraction, processing and use of coal strongly impact water resources, soil, air quality and biota leads to changes in the fauna and flora. Pollutants generated by coal burning have been contaminating plants that grow in area impacted by airborne pollution with high metal contents. Baccharis trimera is popularly consumed as tea, and is widely developed in Candiota (Brazil), one of the most important coal burning regions of the Brazil. This study aims to investigate the phytochemical profile, in vivo genotoxic and mutagenic potential of extracts of B. trimera collected from an exposed region to pollutants generated by coal burning (Candiota City) and other unexposed region (Bagé City), using the Comet assay and micronucleus test in mice and the Salmonella/microsome short-term assay. The HPLC analyses indicated higher levels of flavonoids and phenolic acids for B. trimera aqueous extract from Bagé and absence of polycyclic aromatic hydrocarbons for both extracts. The presence of toxic elements such as cobalt, nickel and manganese was statistically superior in the extract from Candiota. For the Comet assay and micronucleus test, the mice were treated with Candiota and Bagé B. trimera aqueous extracts (500-2000 mg/kg). Significant genotoxicity was observed at higher doses treated with B. trimera aqueous extract from Candiota in liver and peripheral blood cells. Micronuclei were not observed but the results of the Salmonella/microsome short-term assay showed a significant increase in TA98 revertants for B. trimera aqueous extract from Candiota. The extract of B. trimera from Candiota bioacumulated higher levels of trace elements which were associated with the genotoxic effects detected in liver and peripheral blood cells.
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Affiliation(s)
- Ana Paula S Menezes
- Programa de Pós-Graduação em Biologia Celular e Molecular Aplicada a Saúde (PPGBioSaude), Lutheran University of Brazil (ULBRA), Canoas, Rio Grande do Sul, Brazil; Centro de Ciências da Saúde, Universidade da Região da Campanha (URCAMP), Bagé, Rio Grande do Sul, Brazil
| | - Juliana da Silva
- Programa de Pós-Graduação em Biologia Celular e Molecular Aplicada a Saúde (PPGBioSaude), Lutheran University of Brazil (ULBRA), Canoas, Rio Grande do Sul, Brazil; Programa de Pós-Graduação em Genética e Toxicologia Aplicada (PPGGTA.MP), Lutheran University of Brazil (ULBRA), Canoas, Rio Grande do Sul, Brazil.
| | - Camila Fisher
- Programa de Pós-Graduação em Biologia Celular e Molecular Aplicada a Saúde (PPGBioSaude), Lutheran University of Brazil (ULBRA), Canoas, Rio Grande do Sul, Brazil
| | - Fernanda R da Silva
- Programa de Pós-Graduação em Biologia Celular e Molecular Aplicada a Saúde (PPGBioSaude), Lutheran University of Brazil (ULBRA), Canoas, Rio Grande do Sul, Brazil
| | - Juliana M Reyes
- Programa de Pós-Graduação em Biologia Celular e Molecular Aplicada a Saúde (PPGBioSaude), Lutheran University of Brazil (ULBRA), Canoas, Rio Grande do Sul, Brazil
| | - Jaqueline N Picada
- Programa de Pós-Graduação em Biologia Celular e Molecular Aplicada a Saúde (PPGBioSaude), Lutheran University of Brazil (ULBRA), Canoas, Rio Grande do Sul, Brazil; Programa de Pós-Graduação em Genética e Toxicologia Aplicada (PPGGTA.MP), Lutheran University of Brazil (ULBRA), Canoas, Rio Grande do Sul, Brazil
| | - Alice G Ferraz
- Programa de Pós-Graduação em Biologia Celular e Molecular Aplicada a Saúde (PPGBioSaude), Lutheran University of Brazil (ULBRA), Canoas, Rio Grande do Sul, Brazil
| | - Dione S Corrêa
- Programa de Pós-Graduação em Genética e Toxicologia Aplicada (PPGGTA.MP), Lutheran University of Brazil (ULBRA), Canoas, Rio Grande do Sul, Brazil
| | - Suziane M Premoli
- Programa de Pós-Graduação em Genética e Toxicologia Aplicada (PPGGTA.MP), Lutheran University of Brazil (ULBRA), Canoas, Rio Grande do Sul, Brazil
| | - Johnny F Dias
- Laboratório de Implantação Iônica, Instituto de Física, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, Brazil
| | - Claudia T de Souza
- Laboratório de Implantação Iônica, Instituto de Física, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, Brazil
| | - Alexandre de B F Ferraz
- Programa de Pós-Graduação em Biologia Celular e Molecular Aplicada a Saúde (PPGBioSaude), Lutheran University of Brazil (ULBRA), Canoas, Rio Grande do Sul, Brazil; Programa de Pós-Graduação em Genética e Toxicologia Aplicada (PPGGTA.MP), Lutheran University of Brazil (ULBRA), Canoas, Rio Grande do Sul, Brazil.
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Cercato LM, White PAS, Nampo FK, Santos MRV, Camargo EA. A systematic review of medicinal plants used for weight loss in Brazil: Is there potential for obesity treatment? JOURNAL OF ETHNOPHARMACOLOGY 2015; 176:286-96. [PMID: 26520790 DOI: 10.1016/j.jep.2015.10.038] [Citation(s) in RCA: 33] [Impact Index Per Article: 3.3] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Subscribe] [Scholar Register] [Received: 05/23/2015] [Revised: 10/24/2015] [Accepted: 10/26/2015] [Indexed: 05/20/2023]
Abstract
ETHNOPHARMACOLOGICAL RELEVANCE Obesity is a pandemic disease and its prevalence is still increasing. Moreover, it has important costs to public health. In Brazil, many plants are used for weight loss by overweight or obese people, but there is a lack of scientific basis for this practice. Many ethnobotanical studies aiming to characterize this usage have been published, but they are still limited by the region considered and the diversity of the popular knowledge. AIM OF THE STUDY The present study was undertaken to systematically review the ethnobotanical surveys regarding the species utilized to reduce body weight in overweight or obese people in Brazil. METHODS Ethnobotanical surveys related to this usage and performed in Brazilian regions were systematically found in MEDLINE, LILACS and Scopus. RESULTS Thirty-three studies were included in this review. Fifty species were popularly utilized to lose weight. The most cited species were Baccharis trimera (Less.) DC, Annona muricata L. and Hancornia speciosa Gomes. Camellia sinensis (L.) Kuntze and Hibiscus sabdariffa L. were also cited and are supported by either animal or human investigations that indicate some beneficial activity against obesity. However, for the majority of species cited in the included studies, there is no scientific basis that assures the biological effects of this usage. Many studies have demonstrated important effects of these plants on glycemia, serum lipid levels or body weight control in non-obese conditions, which is not sufficient to recommend the use of these plants to reduce body weight in overweight or obese people. CONCLUSIONS Although many plants are popularly used to reduce weight in overweight or obese people in Brazil, there is little scientific evidence corroborating its usage. Based on the ethnobotanical data presented, this review indicates the plants that should be considered for scientifically controlled studies devoted to investigating their effects on obesity.
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Affiliation(s)
- Luana M Cercato
- Department of Physiology, Federal University of Sergipe, (UFS), São Cristóvão 49100-000, SE, Brazil
| | - Pollyanna A S White
- Department of Physiology, Federal University of Sergipe, (UFS), São Cristóvão 49100-000, SE, Brazil
| | - Fernando K Nampo
- Department of Physiology, Federal University of Sergipe, (UFS), São Cristóvão 49100-000, SE, Brazil
| | - Márcio R V Santos
- Department of Physiology, Federal University of Sergipe, (UFS), São Cristóvão 49100-000, SE, Brazil
| | - Enilton A Camargo
- Department of Physiology, Federal University of Sergipe, (UFS), São Cristóvão 49100-000, SE, Brazil.
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20
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Carqueja (Baccharis trimera) Protects against Oxidative Stress and β-Amyloid-Induced Toxicity in Caenorhabditis elegans. OXIDATIVE MEDICINE AND CELLULAR LONGEVITY 2015; 2015:740162. [PMID: 26236426 PMCID: PMC4508469 DOI: 10.1155/2015/740162] [Citation(s) in RCA: 16] [Impact Index Per Article: 1.6] [Reference Citation Analysis] [Abstract] [Track Full Text] [Download PDF] [Figures] [Subscribe] [Scholar Register] [Received: 09/22/2014] [Revised: 01/19/2015] [Accepted: 02/02/2015] [Indexed: 11/23/2022]
Abstract
Carqueja (Baccharis trimera) is a native plant found throughout South America. Several studies have shown that Carqueja has antioxidant activity in vitro, as well as anti-inflammatory, antidiabetic, analgesic, antihepatotoxic, and antimutagenic properties. However, studies regarding its antioxidant potential in vivo are limited. In this study, we used Caenorhabditis elegans as a model to examine the antioxidant effects of a Carqueja hydroalcoholic extract (CHE) on stress resistance and lifespan and to investigate whether CHE has a protective effect in a C. elegans model for Alzheimer's disease. Here, we show for the first time, using in vivo assays, that CHE treatment improved oxidative stress resistance by increasing survival rate and by reducing ROS levels under oxidative stress conditions independently of the stress-related signaling pathways (p38, JNK, and ERK) and transcription factors (SKN-1/Nrf and DAF-16/Foxo) tested here. CHE treatment also increased the defenses against β-amyloid toxicity in C. elegans, in part by increasing proteasome activity and the expression of two heat shock protein genes. Our findings suggest a potential neuroprotective use for Carqueja, supporting the idea that dietary antioxidants are a promising approach to boost the defensive systems against stress and neurodegeneration.
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Menezes APS, Da Silva J, Rossato RR, Santos MS, Decker N, Da Silva FR, Cruz C, Dihl RR, Lehmann M, Ferraz ABF. Genotoxic and biochemical changes in Baccharis trimera induced by coal contamination. ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY 2015; 114:9-16. [PMID: 25590379 DOI: 10.1016/j.ecoenv.2015.01.001] [Citation(s) in RCA: 2] [Impact Index Per Article: 0.2] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Subscribe] [Scholar Register] [Received: 11/06/2014] [Revised: 01/04/2015] [Accepted: 01/05/2015] [Indexed: 06/04/2023]
Abstract
The processing and combustion of coal in thermal power plants release anthropogenic chemicals into the environment. Baccharis trimera is a common plant used in folk medicine that grows readily in soils degraded by coal mining activities. This shrub bioaccumulates metals released into the environment, and thus its consumption may be harmful to health. The purpose of this study was to investigate the phytochemical profile, antioxidant capacity (DPPH), genotoxic (comet assay) and mutagenic potential (CBMN-cyt) in V79 cells of B. trimera aqueous extracts in the coal-mining region of Candiota (Bt-AEC), and in Bagé, a city that does not experience the effects of exposure to coal (Bt-AEB, a reference site). In the comet assay, only Bt-AEC was genotoxic at the highest doses (0.8mg/mL and 1.6mg/mL), compared to the control. For extracts from both areas, mutagenic effects were observed at higher concentrations compared to the control. The cell damage parameters were significantly high in both extracts; however, more striking values were observed for Bt-AEC, up to the dose of 0.8mg/mL. In chemical analysis, no variation was observed in the contents of flavonoids and phenolic compounds, neither the antioxidant activity, which may suggest that DNA damage observed in V79 cells was induced by the presence of coal contaminants absorbed by the plant.
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Affiliation(s)
- A P S Menezes
- Programa de Pós-Graduação em Biologia Celular e Molecular Aplicada a Saúde (PPGBioSaude), Universidade Luterana do Brasil, Canoas, Brazil; Centro de Ciências da Saúde, Universidade da Região da Campanha (URCAMP), Bagé, Rio Grande do Sul, Brazil
| | - J Da Silva
- Programa de Pós-Graduação em Biologia Celular e Molecular Aplicada a Saúde (PPGBioSaude), Universidade Luterana do Brasil, Canoas, Brazil.
| | - R R Rossato
- Programa de Pós-Graduação em Biologia Celular e Molecular Aplicada a Saúde (PPGBioSaude), Universidade Luterana do Brasil, Canoas, Brazil; Centro de Ciências da Saúde, Universidade da Região da Campanha (URCAMP), Bagé, Rio Grande do Sul, Brazil
| | - M S Santos
- Programa de Pós-Graduação em Biologia Celular e Molecular Aplicada a Saúde (PPGBioSaude), Universidade Luterana do Brasil, Canoas, Brazil
| | - N Decker
- Programa de Pós-Graduação em Biologia Celular e Molecular Aplicada a Saúde (PPGBioSaude), Universidade Luterana do Brasil, Canoas, Brazil
| | - F R Da Silva
- Programa de Pós-Graduação em Biologia Celular e Molecular Aplicada a Saúde (PPGBioSaude), Universidade Luterana do Brasil, Canoas, Brazil
| | - C Cruz
- Programa de Pós-Graduação em Biologia Celular e Molecular Aplicada a Saúde (PPGBioSaude), Universidade Luterana do Brasil, Canoas, Brazil
| | - R R Dihl
- Programa de Pós-Graduação em Biologia Celular e Molecular Aplicada a Saúde (PPGBioSaude), Universidade Luterana do Brasil, Canoas, Brazil
| | - M Lehmann
- Programa de Pós-Graduação em Biologia Celular e Molecular Aplicada a Saúde (PPGBioSaude), Universidade Luterana do Brasil, Canoas, Brazil
| | - A B F Ferraz
- Programa de Pós-Graduação em Biologia Celular e Molecular Aplicada a Saúde (PPGBioSaude), Universidade Luterana do Brasil, Canoas, Brazil.
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Vieira MLA, Johann S, Hughes FM, Rosa CA, Rosa LH. The diversity and antimicrobial activity of endophytic fungi associated with medicinal plant Baccharis trimera (Asteraceae) from the Brazilian savannah. Can J Microbiol 2015; 60:847-56. [PMID: 25403761 DOI: 10.1139/cjm-2014-0449] [Citation(s) in RCA: 32] [Impact Index Per Article: 3.2] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 01/18/2023]
Abstract
The fungal endophyte community associated with Baccharis trimera, a Brazilian medicinal plant, was characterized and screened for its ability to present antimicrobial activity. By using molecular methods, we identified and classified the endophytic fungi obtained into 25 different taxa from the phyla Ascomycota and Basidiomycota. The most abundant species were closely related to Diaporthe phaseolorum, Pestalotiopsis sp. 1, and Preussia pseudominima. The differences observed in endophytic assemblages from different B. trimera specimens might be associated with their crude extract activities. Plants that had higher α-biodiversity were also those that contributed more to the regional (γ) diversity. All fungal isolates were cultured and their crude extracts screened to examine the antimicrobial activities. Twenty-three extracts (12.8%) displayed antimicrobial activities against at least one target microorganism. Among these extracts, those obtained from Epicoccum sp., Pestalotiopsis sp. 1, Cochliobolus lunatus, and Nigrospora sp. presented the best minimum inhibitory concentration values. Our results show that the endophytic fungal community associated with the medicinal plant B. trimera included few dominant bioactive taxa, which may represent sources of compounds with antifungal activity. Additionally, the discovery of these bioactive fungi in association with B. trimera suggests that Brazilian plants used as folk medicine may shelter a rich fungal diversity as well as taxa able to produce bioactive metabolites with antimicrobial activities.
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Affiliation(s)
- Mariana L A Vieira
- a Department of Microbiology, Institute of Biological Science, Federal University of Minas Gerais, Belo Horizonte, MG, P.O. Box 486, CEP 31270-901, Brazil
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Antioxidant activity of flavonoids: a QSAR modeling using Fukui indices descriptors. J Mol Model 2014; 20:2476. [DOI: 10.1007/s00894-014-2476-1] [Citation(s) in RCA: 37] [Impact Index Per Article: 3.4] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 05/04/2014] [Accepted: 09/21/2014] [Indexed: 10/24/2022]
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Garcia FADO, Tanae MM, Torres LMB, Lapa AJ, de Lima-Landman MTR, Souccar C. A comparative study of two clerodane diterpenes from Baccharis trimera (Less.) DC. on the influx and mobilization of intracellular calcium in rat cardiomyocytes. PHYTOMEDICINE : INTERNATIONAL JOURNAL OF PHYTOTHERAPY AND PHYTOPHARMACOLOGY 2014; 21:1021-1025. [PMID: 24837474 DOI: 10.1016/j.phymed.2014.04.018] [Citation(s) in RCA: 11] [Impact Index Per Article: 1.0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Subscribe] [Scholar Register] [Received: 09/12/2013] [Revised: 02/21/2014] [Accepted: 04/06/2014] [Indexed: 06/03/2023]
Abstract
Baccharis trimera (Less.) D.C. (Asteraceae) is a medicinal species native to South America and used in Brazilian folk medicine to treat gastrointestinal and liver diseases, kidney disorders and diabetes. The aqueous extract (AE) of the aerial parts of this species presented two mainly constituents: the ent-clerodane diterpene (Fig. 1) and the neo-clerodane diterpene (Fig. 2). The objective of this work was to study their activities on the blockade of Ca(2+)-induced contractions in KCL-depolarized rat portal vein preparations, and on the influx and mobilization of cytosolic calcium in rat cardiomyocytes by fluorescence measurements. The results showed that both the neo- and the ent-clerodane diterpenes reduced the maximal contractions induced by CaCl2, in KCl depolarized rat portal vein preparations, without modifying the EC50. The data on the concentration of cytosolic calcium ([Ca(2+)]c) showed that, while the neo-clerodane diterpene stimulates the mobilization of [Ca(2+)]c in rat cardiomyocytes, this effect was not observed with the ent-clerodane diterpene. On the other hand, the influx of calcium was not altered by the neo-clerodane diterpene, but was reduced in the presence of the ent-clerodane diterpene, indicating that this compound induces a blockade of the voltage-dependent calcium channels.
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Affiliation(s)
- Francisca Adilfa de Oliveira Garcia
- Department of Pharmacology, School of Medicine of the Federal University of São Paulo (UNIFESP), Rua 3 de Maio, 100, CEP: 04044-020 São Paulo, SP, Brazil.
| | - Mirtes Midori Tanae
- Department of Pharmacology, School of Medicine of the Federal University of São Paulo (UNIFESP), Rua 3 de Maio, 100, CEP: 04044-020 São Paulo, SP, Brazil
| | | | - Antônio José Lapa
- Department of Pharmacology, School of Medicine of the Federal University of São Paulo (UNIFESP), Rua 3 de Maio, 100, CEP: 04044-020 São Paulo, SP, Brazil
| | - Maria Teresa Riggio de Lima-Landman
- Department of Pharmacology, School of Medicine of the Federal University of São Paulo (UNIFESP), Rua 3 de Maio, 100, CEP: 04044-020 São Paulo, SP, Brazil
| | - Caden Souccar
- Department of Pharmacology, School of Medicine of the Federal University of São Paulo (UNIFESP), Rua 3 de Maio, 100, CEP: 04044-020 São Paulo, SP, Brazil
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de Oliveira RN, Rehder VLG, Oliveira ASS, Jeraldo VDLS, Linhares AX, Allegretti SM. Anthelmintic activity in vitro and in vivo of Baccharis trimera (Less) DC against immature and adult worms of Schistosoma mansoni. Exp Parasitol 2014; 139:63-72. [PMID: 24602876 DOI: 10.1016/j.exppara.2014.02.010] [Citation(s) in RCA: 58] [Impact Index Per Article: 5.3] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 04/09/2013] [Revised: 02/14/2014] [Accepted: 02/18/2014] [Indexed: 01/12/2023]
Abstract
Although its efficiency against all Schistosoma species, praziquantel (PZQ) shows low efficacy against schistosomula and juvenile stages. The potential for development of resistance to PZQ has justified the search for new alternative chemotherapies. In this scenario, studies to new formulations, more comprehensive and without adverse effects, are being conducted. One viable and promising treatment is the study of medicinal plants as a new approach to the experimental treatment for Schistosomiasis. Amongst all the variety of the medicinal species studied, we can highlight Baccharis trimera (Less) DC, known as "Carqueja-amarga". This paper not only describes the effect of crude dichloromethane extract (DE) and aqueous fraction (AF) obtained from B. trimera, in vitro but also is the first one that investigates the in vivo efficacy of B. trimera against schistosomula, juvenile and adult worms of Schistosoma mansoni BH strain. In the experiment, mice were treated with DE, AF and PZQ (40 and 200mg/kg) over the period of larval development (3 and 30 post-infection; pi), and adult worms (60days post-infection; pi). The in vitro results show that the DE and AF effects are dose-dependents, being the 130μg/mL the most effective one in a shorter period of incubation. The exposure of the in vitro samples over adult parasites were able to inhibit 100% of the oviposition in females. Likewise caused the mortality of the parasites with morphological alterations on the tegument, on the suckers, oral and acetabulum, in both males and females after 6-72h of exposure. Additionally, the in vivo treatments against juvenile and adult infection were more effective compared to the control group untreated. Administrations of AF and DE in day 30pi (juvenile worms) show female worm total burden reductions of 75% and 68% respectively. At the same period of infection reductions of respectively 98% and 97% egg/g in the faeces were seen. In relation to the different egg developmental stages (oogram), the results showed significant reductions, due to the reduction in the number of worms, especially the females. In conclusion, B. trimera exhibits major schistosomicidal effects in vivo against immature and adult worms of S. mansoni, opening up perspectives for future researches on substance or compound isolation and the elucidation of its mechanisms of action.
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Affiliation(s)
| | - Vera Lúcia Garcia Rehder
- Centro Pluridisciplinar de Pesquisas Químicas, Biológicas e Agrícolas, CPQBA, Unicamp, SP, Brazil
| | | | | | - Arício Xavier Linhares
- Instituto de Biologia, Depto. de Biologia Animal, Universidade Estadual de Campinas, Unicamp, SP, Brazil
| | - Silmara Marques Allegretti
- Instituto de Biologia, Depto. de Biologia Animal, Universidade Estadual de Campinas, Unicamp, SP, Brazil.
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Cosenza GP, Somavilla NS, Fagg CW, Brandão MGL. Bitter plants used as substitute of Cinchona spp. (quina) in Brazilian traditional medicine. JOURNAL OF ETHNOPHARMACOLOGY 2013; 149:790-6. [PMID: 23933315 DOI: 10.1016/j.jep.2013.08.004] [Citation(s) in RCA: 10] [Impact Index Per Article: 0.8] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Subscribe] [Scholar Register] [Received: 04/21/2013] [Revised: 07/25/2013] [Accepted: 08/03/2013] [Indexed: 05/12/2023]
Abstract
ETHNOPHARMACOLOGICAL RELEVANCE Bitter tasting plant species are used as tonics and have been previously used to treat intermittent fevers in Brazil, the principal symptom of malaria. Many of these species were named quina and were used as substitutes of Cinchona spp., the source of quinine. AIM OF THE STUDY To present data on these bitter species named quina and to discuss their potential as sources of bioactive substances. MATERIALS AND METHODS Data about the plants were obtained from a survey of the literature and documents written by early naturalists and clinical doctors living in the 18th and 19th centuries in Brazil. Correlated pharmacological studies were obtained from different scientific databases. RESULTS A total of 29 species were recorded. The largest number of species belonged to the Rubiaceae family (14), being Remijia ferruginea (A. St.-Hil) DC. the most representative. Strychnos pseudoquina A. St.-Hil. (Loganiaceae), Hortia brasiliana Vand. ex DC. (Rutaceae) and Solanum pseudoquina A. St.-Hil. (Solanaceae) were also frequently mentioned in the historical bibliography. Pharmacological studies have shown the presence of bitter bioactive substances useful to treat digestive disorders and/or with antimalarial activities, in all of the recorded botanic families. CONCLUSION This study shows that several bitter species named quina were used in the past as substitute of Cinchona spp. and studying these plants can lead to the development of new products.
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Affiliation(s)
- Gustavo P Cosenza
- DATAPLAMT, Museu de História Natural e Jardim Botânico, Universidade Federal de Minas Gerais, Brazil; Faculdade de Farmácia, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil
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Menezes APS, Da Silva J, Roloff J, Reyes J, Debastiani R, Dias JF, Rohr P, de Barros Falcão Ferraz A. Baccharis trimera (Less.) DC as genotoxicity indicator of exposure to coal and emissions from a thermal power plant. ARCHIVES OF ENVIRONMENTAL CONTAMINATION AND TOXICOLOGY 2013; 65:434-441. [PMID: 23733085 DOI: 10.1007/s00244-013-9918-0] [Citation(s) in RCA: 3] [Impact Index Per Article: 0.3] [Reference Citation Analysis] [Abstract] [MESH Headings] [Track Full Text] [Subscribe] [Scholar Register] [Received: 03/05/2013] [Accepted: 05/13/2013] [Indexed: 06/02/2023]
Abstract
During coal combustion, hazardous elements are discharged that impair environmental quality. Plant cover is the first available surface for the atmospheric pollutants in terrestrial ecosystems. The aim of this study was to evaluate genotoxicity in the aqueous extract of the native plant, Baccharis trimera, exposed to coal and emissions from a thermal power plant (coal-fired power plant in Candiota, Brazil), correlating seasonality, wind tunnel predominance, and presence of inorganic elements. The presence of inorganic elements in the aerial parts of B. trimera was analyzed by particle-induced X-ray emission (PIXE) spectrometry, and genotoxicity was evaluated by ex vivo comet assay. The genotoxic effects of aqueous extracts of B. trimera from four sites located in the area around power plant were analyzed by comet assay in peripheral human lymphocytes. Winter samples showed greater levels of metals than summer samples. Genotoxicity was detected in B. trimera extracts collected from the region exposed to extraction and burning coal. Extracts from the site impacted by the dominant wind induced more damage to DNA than those from other sites. Based on our data, we can suggest that in winter the inorganic elements from extraction and burning of coal and carried through the wind tunnel were responsible for the genotoxicity observed in aqueous extract of B. trimera.
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Affiliation(s)
- Ana Paula Simões Menezes
- Centro de Ciências da Saúde, Universidade da Região da Campanha, Bagé, Rio Grande do Sul, Brazil
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de Oliveira CB, Comunello LN, Maciel ÉS, Giubel SR, Bruno AN, Chiela ECF, Lenz G, Gnoatto SCB, Buffon A, Gosmann G. The inhibitory effects of phenolic and terpenoid compounds from Baccharis trimera in Siha cells: differences in their activity and mechanism of action. Molecules 2013; 18:11022-32. [PMID: 24022763 PMCID: PMC6270023 DOI: 10.3390/molecules180911022] [Citation(s) in RCA: 24] [Impact Index Per Article: 2.0] [Reference Citation Analysis] [Abstract] [MESH Headings] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 07/25/2013] [Revised: 09/03/2013] [Accepted: 09/03/2013] [Indexed: 11/16/2022] Open
Abstract
Baccharis trimera is used in folk medicine as a tea for digestive and liver diseases. It possesses anti-inflammatory and antioxidant properties that are related to the presence of phenolic compounds. The aim of this work was to investigate the anti-proliferative properties of phenolic (PHE) and terpenoid (SAP) compounds from B. trimera on human cervical cancer. The treatment of SiHa cells with PHE for 24 h suppressed colony formation in a dose-dependent manner, inhibited proliferation and inhibited cell motility. Although SAP inhibited the proliferation of SiHa cells in a dose-dependent manner, it increased colony formation and did not inhibit cell motility. PHE and SAP also promoted a significant increase in lactate dehydrogenase levels in the culture medium in a dose-dependent manner, indicating a loss of cell membrane integrity. Moreover, PHE promoted necrotic cell death, whereas SAP induced apoptosis. These compounds are new anticancer prototypes due their significant anticancer activity demonstrated herein.
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Affiliation(s)
- Cristiane B. de Oliveira
- Laboratório de Fitoquímica e Síntese Orgânica (LAFIS), Faculdade de Farmácia, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre 90610-000, RS, Brazil; E-Mails: (C.B.O.); (L.N.C.); (É.S.M.); (S.C.B.G.)
- Laboratório de Análises Bioquímicas e Citológicas (LABC), Faculdade de Farmácia, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre 90610-000, RS, Brazil; E-Mail:
| | - Lucimara N. Comunello
- Laboratório de Fitoquímica e Síntese Orgânica (LAFIS), Faculdade de Farmácia, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre 90610-000, RS, Brazil; E-Mails: (C.B.O.); (L.N.C.); (É.S.M.); (S.C.B.G.)
| | - Érica S. Maciel
- Laboratório de Fitoquímica e Síntese Orgânica (LAFIS), Faculdade de Farmácia, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre 90610-000, RS, Brazil; E-Mails: (C.B.O.); (L.N.C.); (É.S.M.); (S.C.B.G.)
| | - Scheron R. Giubel
- Instituto Federal de Educação, Ciência e Tecnologia, Porto Alegre 90030-041, RS, Brazil; E-Mails: (S.R.G.); (A.N.B.)
| | - Alessandra N. Bruno
- Instituto Federal de Educação, Ciência e Tecnologia, Porto Alegre 90030-041, RS, Brazil; E-Mails: (S.R.G.); (A.N.B.)
| | - Eduardo C. F. Chiela
- Laboratório de Sinalização e Plasticidade Celular, Departamento de Biofísica, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre 91501-970, RS, Brazil; E-Mails: (E.C.F.C.); (G.L.)
| | - Guido Lenz
- Laboratório de Sinalização e Plasticidade Celular, Departamento de Biofísica, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre 91501-970, RS, Brazil; E-Mails: (E.C.F.C.); (G.L.)
| | - Simone C. B. Gnoatto
- Laboratório de Fitoquímica e Síntese Orgânica (LAFIS), Faculdade de Farmácia, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre 90610-000, RS, Brazil; E-Mails: (C.B.O.); (L.N.C.); (É.S.M.); (S.C.B.G.)
| | - Andréia Buffon
- Laboratório de Análises Bioquímicas e Citológicas (LABC), Faculdade de Farmácia, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre 90610-000, RS, Brazil; E-Mail:
| | - Grace Gosmann
- Laboratório de Fitoquímica e Síntese Orgânica (LAFIS), Faculdade de Farmácia, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre 90610-000, RS, Brazil; E-Mails: (C.B.O.); (L.N.C.); (É.S.M.); (S.C.B.G.)
- Author to whom correspondence should be addressed; E-Mail: ; Tel.: +55-51-3308-5516; Fax: +55-51-3308-5437
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de Toledo T, da Silva L, Botelho T, Ramos R, de Souza Jr. P, Teixeira A, Freire P, Bento R. Characterization of flavonoid 3-Methoxyquercetin performed by FT-IR and FT-Raman spectroscopies and DFT calculations. J Mol Struct 2012. [DOI: 10.1016/j.molstruc.2012.06.058] [Citation(s) in RCA: 19] [Impact Index Per Article: 1.5] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 01/14/2023]
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de Oliveira CB, Comunello LN, Lunardelli A, Amaral RH, Pires MGS, da Silva GL, Manfredini V, Vargas CR, Gnoatto SCB, de Oliveira JR, Gosmann G. Phenolic enriched extract of Baccharis trimera presents anti-inflammatory and antioxidant activities. Molecules 2012; 17:1113-23. [PMID: 22269829 PMCID: PMC6268486 DOI: 10.3390/molecules17011113] [Citation(s) in RCA: 35] [Impact Index Per Article: 2.7] [Reference Citation Analysis] [Abstract] [MESH Headings] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 12/05/2011] [Revised: 12/27/2011] [Accepted: 01/09/2012] [Indexed: 11/16/2022] Open
Abstract
Baccharis trimera is a plant popularly used as a tea and to treat gastrointestinal diseases and inflammatory processes as well. The total phenolic content was determined and the antioxidant and anti-inflammatory activities of six extracts (dichloromethane, ethyl acetate, butanol, aqueous, saponin and phenolic) from B. trimera were evaluated. Using carrageenan-induced pleurisy as a model of acute inflammation, the phenolic extract at 15 mg/kg decreased significantly the analyzed parameters when compared to the carrageenan group (p < 0.05), thus showing potential anti-inflammatory activity. The total phenolic content and antioxidant activity were evaluated by the Folin-Ciocalteau and DPPH methods, respectively. Phenolic and ethyl acetate extracts presented higher antioxidant activity (p < 0.05) than ascorbic acid. The phenolic extract also showed the highest antioxidant potential in relation to the other extracts, thus suggesting that the antioxidant and anti-inflammatory activities were due to the presence of phenolic compounds.
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Affiliation(s)
- Cristiane B. de Oliveira
- Faculdade de Farmácia, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, RS 90610-000, Brazil; (C.B.O.); (L.N.C.); (S.C.B.G.)
- Laboratório de Biofísica Celular e Inflamação, Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS), Porto Alegre, RS 90619-900, Brazil; (A.L.); (R.H.A.); (M.G.S.P.); (G.L.S.); (J.R.O.)
| | - Lucimara N. Comunello
- Faculdade de Farmácia, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, RS 90610-000, Brazil; (C.B.O.); (L.N.C.); (S.C.B.G.)
| | - Adroaldo Lunardelli
- Laboratório de Biofísica Celular e Inflamação, Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS), Porto Alegre, RS 90619-900, Brazil; (A.L.); (R.H.A.); (M.G.S.P.); (G.L.S.); (J.R.O.)
| | - Robson H. Amaral
- Laboratório de Biofísica Celular e Inflamação, Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS), Porto Alegre, RS 90619-900, Brazil; (A.L.); (R.H.A.); (M.G.S.P.); (G.L.S.); (J.R.O.)
| | - Melissa G. S. Pires
- Laboratório de Biofísica Celular e Inflamação, Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS), Porto Alegre, RS 90619-900, Brazil; (A.L.); (R.H.A.); (M.G.S.P.); (G.L.S.); (J.R.O.)
| | - Gabriela Lucas da Silva
- Laboratório de Biofísica Celular e Inflamação, Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS), Porto Alegre, RS 90619-900, Brazil; (A.L.); (R.H.A.); (M.G.S.P.); (G.L.S.); (J.R.O.)
| | - Vanusa Manfredini
- Faculdade de Farmácia, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, RS 90610-000, Brazil; (C.B.O.); (L.N.C.); (S.C.B.G.)
- Hospital de Clínicas de Porto Alegre, Serviço de Genética Médica, UFRGS, Porto Alegre, RS 93050-150, Brazil; (V.M.); (C.R.V.)
| | - Carmen Regla Vargas
- Faculdade de Farmácia, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, RS 90610-000, Brazil; (C.B.O.); (L.N.C.); (S.C.B.G.)
- Hospital de Clínicas de Porto Alegre, Serviço de Genética Médica, UFRGS, Porto Alegre, RS 93050-150, Brazil; (V.M.); (C.R.V.)
| | - Simone C. B. Gnoatto
- Faculdade de Farmácia, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, RS 90610-000, Brazil; (C.B.O.); (L.N.C.); (S.C.B.G.)
| | - Jarbas R. de Oliveira
- Laboratório de Biofísica Celular e Inflamação, Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS), Porto Alegre, RS 90619-900, Brazil; (A.L.); (R.H.A.); (M.G.S.P.); (G.L.S.); (J.R.O.)
| | - Grace Gosmann
- Faculdade de Farmácia, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, RS 90610-000, Brazil; (C.B.O.); (L.N.C.); (S.C.B.G.)
- Author to whom correspondence should be addressed; ; Tel.: +55-51-3308-5526; Fax: +55-51-3308-5313
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Assay of caffeoylquinic acids in Baccharis trimera by reversed-phase liquid chromatography. J Chromatogr A 2012; 1219:147-53. [DOI: 10.1016/j.chroma.2011.11.042] [Citation(s) in RCA: 24] [Impact Index Per Article: 1.8] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 07/15/2011] [Revised: 11/04/2011] [Accepted: 11/13/2011] [Indexed: 11/22/2022]
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Biondo TMA, Tanae MM, Coletta ED, Lima-Landman MTR, Lapa AJ, Souccar C. Antisecretory actions of Baccharis trimera (Less.) DC aqueous extract and isolated compounds: analysis of underlying mechanisms. JOURNAL OF ETHNOPHARMACOLOGY 2011; 136:368-373. [PMID: 21575703 DOI: 10.1016/j.jep.2011.04.065] [Citation(s) in RCA: 25] [Impact Index Per Article: 1.8] [Reference Citation Analysis] [Abstract] [MESH Headings] [Track Full Text] [Subscribe] [Scholar Register] [Received: 12/22/2010] [Revised: 04/17/2011] [Accepted: 04/30/2011] [Indexed: 05/30/2023]
Abstract
ETHNOPHARMACOLOGICAL RELEVANCE Baccharis trimera (Less.) DC. (Asteraceae) is a species native to South America used in Brazilian folk medicine to treat gastrointestinal and liver diseases, kidney disorders and diabetes. Previous studies from this laboratory confirmed the antacid and antiulcer activities of the plant aqueous extract (AE) in rat and mouse models. AIM OF THE STUDY To investigate the mechanisms involved in the antacid action of AE and isolated compounds from Baccharis trimera. MATERIALS AND METHODS AE was assayed in vivo in cold-restraint stress gastric ulcers and in pylorus-ligated mice. Nine fractions (F2-F10) previously isolated from AE were assayed in vitro on acid secretion measured as [(14)C]-aminopyrine ([(14)C]-AP) accumulation in rabbit gastric glands, and on gastric microsomal H(+), K(+)-ATPase preparations. Chlorogenic acids (F2, F3, F6, F7), flavonoids (F9), an ent-clerodane diterpene (F8) and a dilactonic neo-clerodane diterpene (F10) have been identified in these fractions. RESULTS Intraduodenal injection of AE (1.0 and 2.0 g/kg) in 4h pylorus-ligated mice decreased the volume (20 and 50%) and total acidity (34 and 50%) of acid secretion compared to control values. Administered orally at the same doses AE protected against gastric mucosal lesions induced in mice by restraint at 4°C. Exposure of isolated rabbit gastric glands to fractions F8 (10-100 μM) and F9 (10-300 μg/ml) decreased the basal [(14)C]-AP uptake by 50 and 60% of control (Ratio=6.2±1.1), whereas the remaining fractions were inactive. In the presence of the secretagogues F2 and F4 (30-300 μg/ml) decreased the [(14)C]-AP uptake induced by histamine (His) with a 100-fold lower potency than that of ranitidine. F5 and F6 reduced the [(14)C]-AP uptake stimulated by carbachol (CCh), but they were 10 to 20-fold less potent than atropine. F8 (diterpene 2) and F9 (flavonoids) decreased both the His- and CCh-induced [(14)C]-AP uptake, whereas F10 (diterpene 1) was inactive against the [(14)C]-AP uptake stimulated by secretagogues. Diterpene 2 was the most active of all tested compounds being 7-fold less potent than ranitidine and equipotent to atropine in reducing acid secretion in vitro. This compound also reduced the gastric H(+), K(+)-ATPase activity by 20% of control, while the remaining fractions were inactive on the proton pump in vitro. CONCLUSIONS The results indicate that Baccharis trimera presents constituents that inhibit gastric acid secretion by acting mainly on the cholinergic regulatory pathway. The plant extract also contains compounds that exert moderate inhibition of the histaminergic regulatory pathway of acid secretion and the gastric proton pump. Altogether these active constituents appear to provide effective inhibition of acid secretion in vivo, which may explain the reputed antiulcer activity of the plant extract.
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Affiliation(s)
- Thais Maíra A Biondo
- Department of Pharmacology, Natural Products Section, Escola Paulista de Medicina/Universidade Federal de São Paulo, São Paulo, Brazil
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Grecco SDS, Gimenes L, Ferreira MJ, Romoff P, Favero OA, Zalewski CA, Lago JHG. Triterpenoids and phenolic derivatives from Baccharis uncinella C.DC. (Asteraceae). BIOCHEM SYST ECOL 2010. [DOI: 10.1016/j.bse.2010.12.005] [Citation(s) in RCA: 9] [Impact Index Per Article: 0.6] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 10/18/2022]
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Garcia D, Domingues MV, Rodrigues E. Ethnopharmacological survey among migrants living in the Southeast Atlantic Forest of Diadema, São Paulo, Brazil. JOURNAL OF ETHNOBIOLOGY AND ETHNOMEDICINE 2010; 6:29. [PMID: 21034478 PMCID: PMC2987905 DOI: 10.1186/1746-4269-6-29] [Citation(s) in RCA: 15] [Impact Index Per Article: 1.0] [Reference Citation Analysis] [Abstract] [MESH Headings] [Track Full Text] [Subscribe] [Scholar Register] [Received: 06/24/2010] [Accepted: 10/29/2010] [Indexed: 05/03/2023]
Abstract
BACKGROUND Understanding how people of diverse cultural backgrounds have traditionally used plants and animals as medicinal substances during displacements is one of the most important objectives of ethnopharmacological studies. An ethnopharmacological survey conducted among migrants living in the Southeast Atlantic Forest remnants (Diadema, São Paulo, Brazil) is presented herein. METHODS Ethnographical methods were used to select and interview the migrants, and botanical and zoological techniques were employed to collect the indicated resources. RESULTS We interviewed five migrants who described knowledge on 12 animals and 85 plants. Only 78 plants were present in Diadema, they belong to 37 taxonomic families; 68 were used exclusively for medicinal purposes, whereas 10 were reported to be toxic and/or presented some restriction of use. These taxa were grouped into 12 therapeutic categories (e.g., gastrointestinal disturbances, inflammatory processes or respiratory problems) based on the 41 individual complaints cited by the migrants. While the twelve animal species were used by the migrants to treat nine complaints; these were divided into six categories, the largest of which related to respiratory problems. None of the animal species and only 57 of the 78 plant species analysed in the present study were previously reported in the pharmacological literature; the popular knowledge concurred with academic findings for 30 of the plants. The seven plants [Impatiens hawkeri W. Bull., Artemisia canphorata Vill., Equisetum arvensis L., Senna pendula (Humb. & Bonpl. ex Willd.) H.S. Irwin & Barneby, Zea mays L., Fevillea passiflora Vell. and Croton fuscescens Spreng)] and the two animals (Atta sexdens and Periplaneta americana) that showed maintenance of use among migrants during their displacement in Brazilian territory, have not been studied by pharmacologists yet. CONCLUSIONS Thus, they should be highlighted and focused in further pharmacology and phytochemical studies, since the persistence of their uses can be indicative of bioactive potentials.
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Affiliation(s)
- Daniel Garcia
- Department of Biology, Universidade Federal de São Paulo, Rua Arthur Ridel, 275 CEP, 09941-510, Diadema, S.P., Brazil
| | - Marcus Vinicius Domingues
- Department of Biology, Universidade Federal de São Paulo, Rua Arthur Ridel, 275 CEP, 09941-510, Diadema, S.P., Brazil
| | - Eliana Rodrigues
- Department of Psychobiology, Universidade Federal de São Paulo, Rua Botucatu, 862 - 1º andar - Edifício Biomédicas CEP 04023-062, São Paulo, S.P., Brazil
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Paul EL, Lunardelli A, Caberlon E, de Oliveira CB, Santos RCV, Biolchi V, Bastos CMA, Moreira KB, Nunes FB, Gosmann G, de Oliveira JR. Anti-inflammatory and immunomodulatory effects of Baccharis trimera aqueous extract on induced pleurisy in rats and lymphoproliferation in vitro. Inflammation 2010; 32:419-25. [PMID: 19756999 DOI: 10.1007/s10753-009-9151-1] [Citation(s) in RCA: 40] [Impact Index Per Article: 2.7] [Reference Citation Analysis] [Abstract] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 12/15/2022]
Abstract
Baccharis trimera is a widespread South American plant known as "carqueja". Medicinal teas prepared from the aerial parts of this plant are used in folk medicine in cases of liver diseases and inflammatory processes. We evaluated the effects of aqueous extract of B. trimera in the experimental inflammatory model of carrageenan-induced pleurisy in rat. The injection of carrageenan into the pleural cavity induces an influx of cells and fluid accumulation with a large number of polymorphonuclear leukocytes and increase of protein levels. The inflammation parameters were attenuated when B. trimera (400 and 800 mg/kg, i.p.) was administrated 30 min before the carrageenan. The immunomodulatory effects were evaluated in vitro on human peripheral blood mononuclear cells. The extract in concentration of 25, 50 and 100 mg/mL presented inhibited the T-lymphocytes proliferation stimulated by phytohemagglutinin, but these extract concentrations also presented cytotoxic effect. These results showed that the aqueous extract of B. trimera has anti-inflammatory effect.
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Affiliation(s)
- Elisiane Lozza Paul
- Laboratório de Pesquisa em Biofísica Celular e Inflamação, Faculdade de Biociências, Pontifícia Universidade Católica do Rio Grande do Sul, Porto Alegre, Rio Grande do Sul, Brasil
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Kurdelas RR, Lima B, Tapia A, Egly Feresin G, Gonzalez Sierra M, Rodríguez MV, Zacchino S, Enriz RD, Freile ML. Antifungal activity of extracts and prenylated coumarins isolated from Baccharis darwinii Hook & Arn. (Asteraceae). Molecules 2010; 15:4898-907. [PMID: 20657398 PMCID: PMC6257657 DOI: 10.3390/molecules15074898] [Citation(s) in RCA: 25] [Impact Index Per Article: 1.7] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 06/17/2010] [Revised: 07/01/2010] [Accepted: 07/07/2010] [Indexed: 11/26/2022] Open
Abstract
The petroleum ether extract of Baccharis darwinii showed activity against Cryptococcus neoformans and dermatophytes. Bioactivity-guided fractionation of Baccharis darwinii has resulted in the isolation of three coumarins: 5'-hydroxy aurapten (anisocoumarin H, 1), aurapten (7-geranyloxycoumarin, 2) and 5'-oxoaurapten (diversinin, 3). The structures of these compounds were characterized by spectroscopic methods. These compounds were evaluated for their antimicrobialactivity against a panel of each, bacteria and fungi. Compound 3 showed the best activities against Microsporum gypseum, Trichophyton rubrum and Trichophyton mentagrophytes with MICs = 15.6 microg/mL, followed by compound 1 whose MICs against the same fungi were 62.5 microg/mL. In addition they showed fungicidal rather than fungistatic activity. Both compounds showed moderate activity (MICs = 125 microg/mL) against Cryptococcus neoformans. This is the first report of the presence of compound 1 in B. darwinii.
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Affiliation(s)
- Rita R. Kurdelas
- Laboratorio de Productos Naturales Patagónicos (LAPRONAP), Facultad de Ciencias Naturales, Universidad Nacional de la Patagonia San Juan Bosco, Km 4, CP 9000, Comodoro Rivadavia, Chubut, Argentina; E-Mail: (R.R.K.)
| | - Beatriz Lima
- Instituto de Biotecnología - Instituto de Ciencias Básicas, Universidad Nacional de San Juan, Av. Libertador General San Martín 1109 (O), CP5400, San Juan, Argentina; E-Mails: (A.T.); (B.L.); (G.E.F.)
| | - Alejandro Tapia
- Instituto de Biotecnología - Instituto de Ciencias Básicas, Universidad Nacional de San Juan, Av. Libertador General San Martín 1109 (O), CP5400, San Juan, Argentina; E-Mails: (A.T.); (B.L.); (G.E.F.)
| | - Gabriela Egly Feresin
- Instituto de Biotecnología - Instituto de Ciencias Básicas, Universidad Nacional de San Juan, Av. Libertador General San Martín 1109 (O), CP5400, San Juan, Argentina; E-Mails: (A.T.); (B.L.); (G.E.F.)
| | - Manuel Gonzalez Sierra
- Facultad de Ciencias Bioquímicas y Farmacéuticas, Farmacognosia y Biología Vegetal. Universidad Nacional de Rosario, Suipacha 531, CP2000, Rosario, Argentina; E-Mail: (S.Z.)
| | - María Victoria Rodríguez
- Facultad de Ciencias Bioquímicas y Farmacéuticas, Farmacognosia y Biología Vegetal. Universidad Nacional de Rosario, Suipacha 531, CP2000, Rosario, Argentina; E-Mail: (S.Z.)
| | - Susana Zacchino
- Facultad de Ciencias Bioquímicas y Farmacéuticas, Farmacognosia y Biología Vegetal. Universidad Nacional de Rosario, Suipacha 531, CP2000, Rosario, Argentina; E-Mail: (S.Z.)
| | - Ricardo D. Enriz
- Facultad de Química, Bioquímica y Farmacia. Universidad Nacional de San Luis, Chacabuco 917, CP5700, San Luis, Argentina; E-Mail: (R.D.E.)
| | - Monica L. Freile
- Laboratorio de Productos Naturales Patagónicos (LAPRONAP), Facultad de Ciencias Naturales, Universidad Nacional de la Patagonia San Juan Bosco, Km 4, CP 9000, Comodoro Rivadavia, Chubut, Argentina; E-Mail: (R.R.K.)
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Pádua BDC, Silva LD, Rossoni Júnior JV, Humberto JL, Chaves MM, Silva ME, Pedrosa ML, Costa DC. Antioxidant properties of Baccharis trimera in the neutrophils of Fisher rats. JOURNAL OF ETHNOPHARMACOLOGY 2010; 129:381-386. [PMID: 20430095 DOI: 10.1016/j.jep.2010.04.018] [Citation(s) in RCA: 19] [Impact Index Per Article: 1.3] [Reference Citation Analysis] [Abstract] [MESH Headings] [Track Full Text] [Subscribe] [Scholar Register] [Received: 11/13/2009] [Revised: 04/01/2010] [Accepted: 04/06/2010] [Indexed: 05/29/2023]
Abstract
ETHNOPHARMACOLOGICAL RELEVANCE Baccharis trimera (Less.) (Asteraceae) is a native plant of Brazil. Also known as "carqueja", it has been popularly used to treat liver diseases, diabetes, as well as digestive disorders. Other studies have described the hepatoprotective, antioxidant and anti-inflammatory activities of the species. AIM OF THE STUDY The aim of the present study was to investigate the antioxidant properties of Baccharis trimera in the neutrophils of Fisher rats in both in vitro and in vivo experimental models. MATERIAL AND METHODS In the in vitro assay, the neutrophils of male rats were isolated and incubated with Baccharis trimera extract at concentrations of 0.5, 5.0 and 50.0 microg/mL. In the in vivo assay, male rats were first treated with crude extract 600 mg/kg body weight of Baccharis trimera or with 50 mg/kg body weight of quercetin (reference substance) and then treated with 835 mg/kg of acetaminophen (APAP) after 24 h. RESULTS The hydroethanolic extract of Baccharis trimera reduced the release of reactive oxygen species in the neutrophils in both the in vitro and in vivo experimental models. Therefore confirming its antioxidant effect. CONCLUSION The results of this study confirm the antioxidant effect of Baccharis trimera.
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Affiliation(s)
- Bruno da Cruz Pádua
- Programa de Pós-graduação em Ciências Biológicas do Núcleo de Pesquisas em Ciências Biológicas-NUPEB, Universidade Federal de Ouro Preto (UFOP), Ouro Preto, MG, Brazil
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Supercritical fluid extraction of carqueja (Baccharis trimera) oil: Process parameters and composition profiles. FOOD AND BIOPRODUCTS PROCESSING 2009. [DOI: 10.1016/j.fbp.2009.01.001] [Citation(s) in RCA: 19] [Impact Index Per Article: 1.2] [Reference Citation Analysis] [Track Full Text] [Subscribe] [Scholar Register] [Indexed: 01/12/2023]
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Rodrigues CRF, Dias JH, Semedo JG, da Silva J, Ferraz ABF, Picada JN. Mutagenic and genotoxic effects of Baccharis dracunculifolia (D.C.). JOURNAL OF ETHNOPHARMACOLOGY 2009; 124:321-324. [PMID: 19397982 DOI: 10.1016/j.jep.2009.04.022] [Citation(s) in RCA: 17] [Impact Index Per Article: 1.1] [Reference Citation Analysis] [Abstract] [MESH Headings] [Track Full Text] [Subscribe] [Scholar Register] [Received: 12/30/2008] [Revised: 04/06/2009] [Accepted: 04/19/2009] [Indexed: 05/27/2023]
Abstract
Baccharis dracunculifolia (D.C.) (Asteraceae), a native plant to Brazil known as "vassourinha" or "alecrim-do-campo", is the most important botanical source of a Brazilian propolis called green propolis. The leaf extracts of this plant have been used to treat liver and digestive disorders. It has been recognized that green propolis can induce mutagenic effects at high doses, but no study reporting possible mutagenic effects by Baccharis dracunculifolia extracts in the maximum tolerated dose has been conducted. The aim of the present study was to investigate the genotoxic and mutagenic effects of this plant in vivo. Adult CF-1 mice were treated with 0.5g/kg, 1.0g/kg or 2.0g/kg of an aqueous extract of Baccharis dracunculifolia by gavage for 3 consecutive days. Blood and liver samples were collected to detect DNA damage using the comet assay, while bone marrow samples were used to assess chromosome mutations by the micronucleus test. The extract increased the DNA damage in blood and liver tissues and the frequency of micronucleus in bone marrow. These findings suggest genotoxic and mutagenic effects of Baccharis dracunculifolia comparable to green propolis in mice.
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Affiliation(s)
- Carmem R F Rodrigues
- Programa de Pós-Graduação em Genética e Toxicologia Aplicada, Universidade Luterana do Brasil (ULBRA), Canoas, RS, Brazil
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Chen J, Liu Y, Shi YP. Determination of flavonoids in the flowers of Paulownia tomentosa by high-performance liquid chromatography. JOURNAL OF ANALYTICAL CHEMISTRY 2009. [DOI: 10.1134/s1061934809030137] [Citation(s) in RCA: 7] [Impact Index Per Article: 0.4] [Reference Citation Analysis] [Track Full Text] [Subscribe] [Scholar Register] [Indexed: 11/23/2022]
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Budel JM, Duarte MDR. Análise morfoanatômica comparativa de duas espécies de carqueja: Baccharis microcephala DC. e B. trimera (Less.) DC., Asteraceae. BRAZ J PHARM SCI 2009. [DOI: 10.1590/s1984-82502009000100009] [Citation(s) in RCA: 11] [Impact Index Per Article: 0.7] [Reference Citation Analysis] [Abstract] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 01/12/2023] Open
Abstract
A identificação de espécies de Baccharis que são constituídas de cladódios, ou seja, caules alados, apresenta dificuldades até mesmo para especialistas em taxonomia vegetal. Baccharis microcephala e B. trimera pertencem ao grupo Trimera e são conhecidas vulgarmente como carquejas. Na medicina popular, é comum o uso de espécies aladas para a aplicação terapêutica como diurético, hepatoprotetor e digestivo. Baccharis trimera consta na F. Bras. IV e possui atividades antiinflamatória, analgésica, bacteriostática, bactericida e antidiabética. As espécies B. trimera e B. microcephala são freqüentemente confundidas e utilizadas pela população para as mesmas finalidades terapêuticas. Objetivou-se estudar a morfologia externa e a anatomia dos cladódios dessas espécies, com a finalidade de se obterem dados que auxiliem na diferenciação e conseqüentemente na identificação das mesmas, contribuindo para o controle de qualidade de fitoterápicos, além de auxiliar na caracterização do grupo taxonômico. O material botânico foi submetido às microtécnicas fotônica e eletrônica de varredura usuais. As duas espécies são trialadas, sendo que em B. microcephala as alas são estreitas, praticamente contínuas em toda a extensão caulinar, enquanto que em B. trimera as alas são mais largas e interrompidas. As características morfológicas permitem a diferenciação dessas duas espécies, principalmente os tricomas tectores.
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Mitra S, Gole M, Samajdar K, Sur RK, Chakraborty BN. Antihepatotoxic Activity ofChelidonium majus. ACTA ACUST UNITED AC 2008. [DOI: 10.3109/13880209209053974] [Citation(s) in RCA: 14] [Impact Index Per Article: 0.8] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 12/15/2022]
Affiliation(s)
- S. Mitra
- Animal Physiology and Biochemistry Laboratory, Zoology Department, Calcutta University, 35, Ballygunge Circular Road, Calcutta, 700 019, India
| | - M Gole
- Animal Physiology and Biochemistry Laboratory, Zoology Department, Calcutta University, 35, Ballygunge Circular Road, Calcutta, 700 019, India
| | - K. Samajdar
- Animal Physiology and Biochemistry Laboratory, Zoology Department, Calcutta University, 35, Ballygunge Circular Road, Calcutta, 700 019, India
| | - R. K. Sur
- Animal Physiology and Biochemistry Laboratory, Zoology Department, Calcutta University, 35, Ballygunge Circular Road, Calcutta, 700 019, India
| | - B. N. Chakraborty
- Animal Physiology and Biochemistry Laboratory, Zoology Department, Calcutta University, 35, Ballygunge Circular Road, Calcutta, 700 019, India
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Lameira J, Alves C, Santos L, Santos A, de Almeida Santos R, Souza J, Silva C, da Silva A. A combined X-ray and theoretical study of flavonoid compounds with anti-inflammatory activity. ACTA ACUST UNITED AC 2008. [DOI: 10.1016/j.theochem.2008.04.029] [Citation(s) in RCA: 10] [Impact Index Per Article: 0.6] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 11/17/2022]
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Grance SRM, Teixeira MA, Leite RS, Guimarães EB, de Siqueira JM, de Oliveira Filiu WF, de Souza Vasconcelos SB, do Carmo Vieira M. Baccharis trimera: effect on hematological and biochemical parameters and hepatorenal evaluation in pregnant rats. JOURNAL OF ETHNOPHARMACOLOGY 2008; 117:28-33. [PMID: 18346859 DOI: 10.1016/j.jep.2007.12.020] [Citation(s) in RCA: 21] [Impact Index Per Article: 1.2] [Reference Citation Analysis] [Abstract] [MESH Headings] [Track Full Text] [Subscribe] [Scholar Register] [Received: 07/02/2007] [Revised: 12/24/2007] [Accepted: 12/31/2007] [Indexed: 05/26/2023]
Abstract
AIM OF THE STUDY This investigation evaluated the effect of a hydroethanolic extract of Baccharis trimera on pregnant Wistar rats, once the plant is well-known consumed in pregnancy and little is known on its potentially toxic effects on pregnant women. MATERIAL AND METHODS Thirty-five female rats were distributed into three groups. Those in treatments 1 and 2 were given 8.4 mg/kg of the extract orally from gestational day (GD) 1 to 19 and from GD 6 to 15, respectively, whereas those in the control group received distilled water orally from GD 1 to 19. Body weights were recorded on GD 1, 6, 15, and 20. On GD 20 animals were anesthetized, blood samples were collected and maternal livers, kidneys, and spleens were weighed and processed for histological studies. RESULTS No clinical signs of maternal toxicity and no changes in hematological parameters were observed. Urea levels and kidney weights differed significantly between animals receiving treatment 1 and controls. Histopathological alterations were found in kidneys and livers in both treatment groups. CONCLUSIONS The hydroethanolic extract of Baccharis trimera administered to pregnant rats at 8.4 mg/kg was toxic to maternal kidney and liver cells, although such alterations are reversible once administration is discontinued.
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Affiliation(s)
- Simone Reschke Mendes Grance
- Program in Animal Science, Universidade Federal de Mato Grosso do Sul (UFMS), Cx. Postal 549, Campo Grande, MS 79070-900, Brazil.
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Herrerias T, de Oliveira BH, Gomes MA, de Oliveira MB, Carnieri EG, Cadena SM, Martinez GR, Rocha ME. Eupafolin: Effect on mitochondrial energetic metabolism. Bioorg Med Chem 2008; 16:854-61. [DOI: 10.1016/j.bmc.2007.10.029] [Citation(s) in RCA: 8] [Impact Index Per Article: 0.5] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 06/15/2007] [Revised: 09/25/2007] [Accepted: 10/10/2007] [Indexed: 12/29/2022]
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Mendes FR, Carlini EA. Brazilian plants as possible adaptogens: an ethnopharmacological survey of books edited in Brazil. JOURNAL OF ETHNOPHARMACOLOGY 2007; 109:493-500. [PMID: 17030478 DOI: 10.1016/j.jep.2006.08.024] [Citation(s) in RCA: 38] [Impact Index Per Article: 2.1] [Reference Citation Analysis] [Abstract] [MESH Headings] [Track Full Text] [Subscribe] [Scholar Register] [Received: 12/29/2005] [Revised: 08/16/2006] [Accepted: 08/26/2006] [Indexed: 05/12/2023]
Abstract
In a survey, from Brazilian books, we searched plants that are in popular use for purposes resembling those of an adaptogen. This study focused on 24 books by authors from diverse regions in the country, resulting in a total of 1317 citations of uses related to a possible adaptogen effect from approximately 766 plants. Only species native to Brazil, cited in at least four books, were selected, resulting a total of 33 species, belonging to 24 families. Of these, four species have been studied previously in relation to effects that are considered as part of an adaptogen effect (anti-stress, memory enhancement, increased physical and/or sexual performance): Heteropterys aphrodisiaca (Malpighiaceae), Paullinia cupana (Sapindaceae), Ptychopetalum olacoides (Olacaceae), and Turnera diffusa (Turneraceae). Three others--Pfaffia glomerata, Pfaffia paniculata (Amaranthaceae), and Trichilia catigua (Meliaceae)--have also been the object of pharmacological studies that support their use as a possible adaptogen, but they are listed in less than four books. The overall results obtained in the present review of Brazilian folk literature reveals that Brazil is rich in plants with potential adaptogen-like effect, but lacks pharmacological studies (mostly clinical ones) to confirm these therapeutic properties.
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Mendes FR, Tabach R, Carlini EA. Evaluation ofBaccharis trimera andDavilla rugosa in tests for adaptogen activity. Phytother Res 2007; 21:517-22. [PMID: 17326041 DOI: 10.1002/ptr.2080] [Citation(s) in RCA: 14] [Impact Index Per Article: 0.8] [Reference Citation Analysis] [Abstract] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 11/09/2022]
Abstract
Baccharis trimera (Asteraceae) and Davilla rugosa (Dilleniaceae) are used popularly as tonics, aphrodisiacs and for stomach ailments, among other uses. Hydroalcohol extracts of the aerial parts of both plants were investigated with regard to their chemical constitution and their pharmacological activity in tests that evaluate adaptogen activity. Alkaloids, flavonoids, saponins, polyphenols/tannins and coumarins were identified in both extracts, while lignans were found only in the extract of Davilla rugosa. This extract presented also a marked antioxidant activity and exerted a moderate antiulcer effect in rats submitted to cold immobilization stress. It did not, however, inhibit the increase in the levels of ACTH and corticosterone induced by stress. Moreover, the Davilla rugosa did not improve the physical performance of mice submitted to forced exercise and the learning time of old rats in the T-maze, neither did it reduce the blood viscosity of the old animals. Conversely, the Baccharis trimera extract only presented a moderate antioxidant activity, without any positive effect on the other tests. These results point to the absence of an adaptogen activity of Baccharis trimera, with some effects that could be related to such an activity as regards the Davilla rugosa.
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Dabaghi-Barbosa P, Mariante Rocha A, Franco da Cruz Lima A, Heleno de Oliveira B, Benigna Martinelli de Oliveira M, Gunilla Skare Carnieri E, Cadena SMSC, Eliane Merlin Rocha M. Hispidulin: antioxidant properties and effect on mitochondrial energy metabolism. Free Radic Res 2005; 39:1305-15. [PMID: 16298860 DOI: 10.1080/13561820500177659] [Citation(s) in RCA: 25] [Impact Index Per Article: 1.3] [Reference Citation Analysis] [Abstract] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 10/25/2022]
Abstract
Hispidulin (6-methoxy-5,7,4'-trihydroxyflavone) and eupafolin (6-methoxy-5,7,3',4'-tetrahydroxyflavone), are flavonoids found in the leaves of Eupatorium litoralle. They have recognized antioxidant and antineoplastic properties, although their action mechanisms have not been previously described. We now report the effects of hispidulin on the oxidative metabolism of isolated rat liver mitochondria (Mit) and have also investigated the prooxidant and antioxidant capacity of both flavonoids. Hispidulin (0.05-0.2 mM) decreased the respiratory rate in state III and stimulated it in state IV, when glutamate or succinate was used as oxidizable substrate. Hispidulin inhibited enzymatic activities between complexes I and III of the respiratory chain. In broken Mit hispidulin (0.2 mM) slightly inhibited ATPase activity (25%). However, when intact Mit were used, the flavonoid stimulated this activity by 100%. Substrate energized mitochondrial swelling was markedly inhibited by hispidulin. Both hispidulin and eupafolin were able to promote iron release from ferritin, this effect being more accentuated with eupafolin with the suggestion of a possible involvement of H2O2 in the process. Hispidulin was incapable of donating electrons to the stable free radical DPPH, while eupafolin reacted with it in a similar way to ascorbic acid. The results indicate that hispidulin as an uncoupler of oxidative phosphorylation, is able to release iron from ferritin, but has distinct prooxidant and antioxidant properties when compared to eupafolin.
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Affiliation(s)
- Priscila Dabaghi-Barbosa
- Departamento de Bioquímica, Universidade Federal do Paraná, CP 19046, Curitiba, PR, CEP 81531-590, Brazil
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Simões-Pires CA, Queiroz EF, Henriques AT, Hostettmann K. Isolation and on-line identification of antioxidant compounds from three Baccharis species by HPLC-UV-MS/MS with post-column derivatisation. PHYTOCHEMICAL ANALYSIS : PCA 2005; 16:307-14. [PMID: 16223086 DOI: 10.1002/pca.826] [Citation(s) in RCA: 44] [Impact Index Per Article: 2.2] [Reference Citation Analysis] [Abstract] [MESH Headings] [Track Full Text] [Subscribe] [Scholar Register] [Indexed: 05/04/2023]
Abstract
The aqueous extracts of aerial parts of Baccharis trimera (Less.) DC., B. crispa Spreng. and B. usterii Heering (Asteraceae) displayed significant radical scavenging activity in a diphenylpicrylhydrazole (DPPH)/TLC assay. In order to rapidly identify the active principles, the crude extracts were analysed by HPLC-UV, and an HPLC-micro-fractionation of the extract of B. usterii was performed. Six quinic acids derivatives (1-6) were isolated from B. usterii by MPLC. The fractions were monitored by DPPH/TLC assay and a series of radical-scavenging quinic acid derivatives could be identified. The comparison of the HPLC profiles of the extracts of B. usterii, B. trimera and B. crispa was performed. In order to obtain complementary on-line structural information for all peaks of interest, HPLC-MS/MS together with HPLC-UV involving post-column addition of UV shift reagents was undertaken on the crude extract. The interpretation of these data permitted the on-line identification of known compounds, some of which are reported for the first time in this plant.
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Affiliation(s)
- Cláudia A Simões-Pires
- Programa de Pós-graduação em Ciências Farmacêuticas, Universidade Federal do Rio Grande do Sul, Porto Alegre-RS, Brazil
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Martinez MJA, Bessa AL, Benito PB. Biologically Active Substances from the Genus Baccharis L. (Compositae). BIOACTIVE NATURAL PRODUCTS (PART K) 2005. [DOI: 10.1016/s1572-5995(05)80045-6] [Citation(s) in RCA: 11] [Impact Index Per Article: 0.6] [Reference Citation Analysis] [Track Full Text] [Subscribe] [Scholar Register] [Indexed: 02/12/2023]
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