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Alfei S, Zuccari G. Carbon-Nanotube-Based Nanocomposites in Environmental Remediation: An Overview of Typologies and Applications and an Analysis of Their Paradoxical Double-Sided Effects. J Xenobiot 2025; 15:76. [PMID: 40407540 PMCID: PMC12101166 DOI: 10.3390/jox15030076] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 04/09/2025] [Revised: 05/05/2025] [Accepted: 05/14/2025] [Indexed: 05/26/2025] Open
Abstract
Incessant urbanization and industrialization have resulted in several pollutants being increasingly produced and continuously discharged into the environment, altering its equilibrium, with a high risk for living organisms' health. To restore it, new advanced materials for remediating gas streams, polluted soil, water, wastewater, groundwater and industrial waste are continually explored. Carbon-based nanomaterials (CNMs), including quantum dots, nanotubes, fullerenes and graphene, have displayed outstanding effectiveness in the decontamination of the environment by several processes. Carbon nanotubes (CNTs), due to their nonpareil characteristics and architecture, when included in absorbents, filter membranes, gas sensors, etc., have significantly improved the efficiency of these technologies in detecting and/or removing inorganic, organic and gaseous xenobiotics and pathogens from air, soil and aqueous matrices. Moreover, CNT-based membranes have displayed significant potential for efficient, fast and low-energy water desalination. However, despite CNTs serving as very potent instruments for environmental detoxification, their extensive utilization could, paradoxically, be highly noxious to the environment and, therefore, humans, due to their toxicity. The functionalization of CNTs (F-CNTs), in addition to further enhancing their absorption capacity and selectivity, has increased their hydrophilicity, thus minimizing their toxicity and carcinogenic effects. In this scenario, this review aims to provide evidence of both the enormous potential of CNTs in sustainable environmental remediation and the concerning hazards to the environment and living organisms that could derive from their extensive and uncontrolled utilization. To this end, an introduction to CNTs, including their eco-friendly production from biomass, is first reported. Several literature reports on CNTs' possible utilization for environmental remediation, their potential toxicity due to environmental accumulation and the challenges of their regeneration are provided using several reader-friendly tools, to better capture readers' attention and make reading easier.
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Affiliation(s)
- Silvana Alfei
- Department of Pharmacy (DIFAR), University of Genoa, Viale Cembrano, 4, 16148 Genoa, Italy
| | - Guendalina Zuccari
- Department of Pharmacy (DIFAR), University of Genoa, Viale Cembrano, 4, 16148 Genoa, Italy
- Laboratory of Experimental Therapies in Oncology, IRCCS Istituto Giannina Gaslini, Via G. Gaslini 5, 16147 Genoa, Italy
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2
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Alfei S, Schito GC. Antimicrobial Nanotubes: From Synthesis and Promising Antimicrobial Upshots to Unanticipated Toxicities, Strategies to Limit Them, and Regulatory Issues. NANOMATERIALS (BASEL, SWITZERLAND) 2025; 15:633. [PMID: 40278498 PMCID: PMC12029864 DOI: 10.3390/nano15080633] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Subscribe] [Scholar Register] [Received: 03/19/2025] [Revised: 04/15/2025] [Accepted: 04/18/2025] [Indexed: 04/26/2025]
Abstract
Nanotubes (NTs) are nanosized tube-like structured materials made from various substances such as carbon, boron, or silicon. Carbon nanomaterials (CNMs), including carbon nanotubes (CNTs), graphene/graphene oxide (G/GO), and fullerenes, have good interatomic interactions and possess special characteristics, exploitable in several applications because of the presence of sp2 and sp3 bonds. Among NTs, CNTs are the most studied compounds due to their nonpareil electrical, mechanical, optical, and biomedical properties. Moreover, single-walled carbon nanotubes (SWNTs) have, in particular, demonstrated high ability as drug delivery systems and in transporting a wide range of chemicals across membranes and into living cells. Therefore, SWNTs, more than other NT structures, have generated interest in medicinal applications, such as target delivery, improved imaging, tissue regeneration, medication, and gene delivery, which provide nanosized devices with higher efficacy and fewer side effects. SWNTs and multi-walled CNTs (MWCNTs) have recently gained a great deal of attention for their antibacterial effects. Unfortunately, numerous recent studies have revealed unanticipated toxicities caused by CNTs. However, contradictory opinions exist regarding these findings. Moreover, the problem of controlling CNT-based products has become particularly evident, especially in relation to their large-scale production and the nanosized forms of the carbon that constitute them. Important directive rules have been approved over the years, but further research and regulatory measures should be introduced for a safer production and utilization of CNTs. Against this background, and after an overview of CNMs and CNTs, the antimicrobial properties of pristine and modified SWNTs and MWCNTs as well as the most relevant in vitro and in vivo studies on their possible toxicity, have been reported. Strategies and preventive behaviour to limit CNT risks have been provided. Finally, a debate on regulatory issues has also been included.
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Affiliation(s)
- Silvana Alfei
- Department of Pharmacy (DIFAR), University of Genoa, Viale Cembrano, 4, 16148 Genoa, Italy
| | - Gian Carlo Schito
- Department of Surgical Sciences and Integrated Diagnostics (DISC), University of Genoa, Viale Benedetto XV, 6, 16132 Genova, Italy;
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3
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Suni J, Valkama S, Peltola E. Save Your Tears for the Toxicity Assays-Carbon Nanotubes Still Fooling Scientists. ACS OMEGA 2025; 10:5554-5562. [PMID: 39989827 PMCID: PMC11840583 DOI: 10.1021/acsomega.4c08211] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Grants] [Track Full Text] [Figures] [Subscribe] [Scholar Register] [Received: 09/06/2024] [Revised: 01/23/2025] [Accepted: 01/29/2025] [Indexed: 02/25/2025]
Abstract
The extensive study of carbon nanotube (CNT) toxicity stems from their widespread application across various fields. The toxicity of CNTs is commonly assessed using cell viability assays based on tetrazolium salts, such as the MTT assay. ISO 10993-5 outlines the MTT assay and related in vitro cytotoxicity tests as international standards. However, nearly two decades ago, it was observed that MTT interacts with CNTs, potentially yielding inaccurate results. Despite this, the MTT assay remains the most widely used method for studying CNT toxicity in vitro today. Here, we demonstrate that six commonly used tetrazolium salts in cell viability assays-MTT, MTS, INT, XTT, WST-1, and WST-8- interfere with both single-walled nanotubes (SWCNTs) and multiwalled carbon nanotubes (MWCNTs). According to ISO 10993-5, cell viability percentages below 70% indicate cytotoxicity. At the standard testing duration of 3 h, the absorbance values in the presence of 5 mg/mL of either SWCNT or MWCNT decreased to below 70% relative to the control. At a lower concentration of 0.5 mg/mL, the effect was less pronounced, with the absorbance decreasing to an average of 84% compared to the control. Our results suggest that none of these cell viability assays alone offers a fully reliable method for evaluating CNT toxicity, especially with high CNT concentrations. Therefore, it is essential to carefully assess which in vitro methods are truly suitable for CNT toxicity studies.
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Affiliation(s)
- Johanna Suni
- Department of Mechanical
and Materials Engineering, University of
Turku, Turku 20500, Finland
| | - Salli Valkama
- Department of Mechanical
and Materials Engineering, University of
Turku, Turku 20500, Finland
| | - Emilia Peltola
- Department of Mechanical
and Materials Engineering, University of
Turku, Turku 20500, Finland
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4
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Ahmed OHM, Naiki-Ito A, Takahashi S, Alexander WT, Alexander DB, Tsuda H. A Review of the Carcinogenic Potential of Thick Rigid and Thin Flexible Multi-Walled Carbon Nanotubes in the Lung. NANOMATERIALS (BASEL, SWITZERLAND) 2025; 15:168. [PMID: 39940144 PMCID: PMC11820818 DOI: 10.3390/nano15030168] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Subscribe] [Scholar Register] [Received: 01/01/2025] [Revised: 01/16/2025] [Accepted: 01/20/2025] [Indexed: 02/14/2025]
Abstract
The carcinogenic potential of MWCNTs is not well defined. Currently, IARC has classified MWCNT-7 as a Group 2 B material, possibly carcinogenic to humans, and all other MWCNTs as Group 3 materials, inadequate evidence in experimental animals for their carcinogenicity and not classifiable as to their carcinogenicity to humans. In this review we discuss studies that investigated the lung toxicity of well characterized MWCNTs in mice and rats. Intraperitoneal and intrascrotal injection studies identified rigid MWCNTs as hazardous materials. The assessment of lung toxicity of MWCNTs in short and medium term instillation and inhalation studies were not conclusive; therefore, these studies do not confirm the hazard of MWCNTs. However, two-year carcinogenicity studies indicate that MWCNT-7 and other MWCNTs, both thick rigid MWCNTs and thin flexible MWCNTs, are carcinogenic in test animals. Therefore, the carcinogenicity of MWCNTs in experimental animals should be reassessed.
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Affiliation(s)
- Omnia Hosny Mohamed Ahmed
- Nanotoxicology Project, Nagoya City University, Nagoya 467-8603, Japan; (O.H.M.A.); (W.T.A.)
- Department of Experimental Pathology and Tumor Biology, Graduate School of Medical Sciences, Nagoya City University, Nagoya 467-8601, Japan; (A.N.-I.); (S.T.)
- Department of Forensic Medicine and Clinical Toxicology, Faculty of Medicine, Aswan University, Aswan 81528, Egypt
| | - Aya Naiki-Ito
- Department of Experimental Pathology and Tumor Biology, Graduate School of Medical Sciences, Nagoya City University, Nagoya 467-8601, Japan; (A.N.-I.); (S.T.)
| | - Satoru Takahashi
- Department of Experimental Pathology and Tumor Biology, Graduate School of Medical Sciences, Nagoya City University, Nagoya 467-8601, Japan; (A.N.-I.); (S.T.)
| | - William T. Alexander
- Nanotoxicology Project, Nagoya City University, Nagoya 467-8603, Japan; (O.H.M.A.); (W.T.A.)
| | - David B. Alexander
- Nanotoxicology Project, Nagoya City University, Nagoya 467-8603, Japan; (O.H.M.A.); (W.T.A.)
| | - Hiroyuki Tsuda
- Nanotoxicology Project, Nagoya City University, Nagoya 467-8603, Japan; (O.H.M.A.); (W.T.A.)
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5
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Zheng L, Li J, Huang J, Feng W, Zhan Y, Liu D. Near Real-Time Measurement of Airborne Carbon Nanotubes with Metals Using Raman-Spark Emission Spectroscopy. APPLIED SPECTROSCOPY 2025:37028241307258. [PMID: 39772927 DOI: 10.1177/00037028241307258] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Subscribe] [Scholar Register] [Indexed: 01/11/2025]
Abstract
We present a near real-time measurement method that combines Raman and spark emission spectroscopy to quantitatively analyze the molecular structure of airborne single-walled carbon nanotubes (SWCNTs) and multi-walled carbon nanotubes (MWCNTs), as well as detect toxic metals within CNTs. A corona-based aerosol microconcentrator was used for airborne CNTs sampling to enhance the measurement accuracy and sensitivity. The intensity of the characteristic Raman bands of CNTs and atomic emission lines of metals exhibited a linear relationship with the analyte mass, yielding high coefficient R2 values. By carefully selecting appropriate signal peaks for calibration, we achieved a limit of detection (LOD) in terms of air concentration as low as 0.09 μg/m3 for SWCNT and 0.81 μg/m3 for MWCNT with a sampling time of 10 min. Additionally, our method exhibited excellent performance in measuring metals, with a mass LOD of 0.8-0.9 ng for Co and Ni and a mass LOD of 35.09 ng for Fe. The method performed well for the measurement of CNT and relevant metal composition with advantages of near real-time monitoring, low LOD, and portable use, making it a valuable tool for various applications in nanomaterial analysis.
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Affiliation(s)
- Lina Zheng
- Jiangsu Engineering Research Center for Dust Control and Occupational Protection, China University of Mining and Technology, Xuzhou, Jiangsu, China
| | - Jialin Li
- School of Safety Engineering, China University of Mining and Technology, Xuzhou, Jiangsu, China
| | - Jing Huang
- School of Safety Engineering, China University of Mining and Technology, Xuzhou, Jiangsu, China
| | - Wenting Feng
- School of Safety Engineering, China University of Mining and Technology, Xuzhou, Jiangsu, China
| | - Yuhan Zhan
- School of Architecture and Design, China University of Mining and Technology, Xuzhou, Jiangsu, China
| | - Dou Liu
- Jiangsu Engineering Research Center for Dust Control and Occupational Protection, China University of Mining and Technology, Xuzhou, Jiangsu, China
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Cheng M, Liang X, Cui L, Guan D, Qu Y, Zhao J, Guan K. Magnesium-based nanocomposites for orthopedic applications: A review. JOURNAL OF MAGNESIUM AND ALLOYS 2024; 12:4335-4362. [DOI: 10.1016/j.jma.2024.11.028] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Track Full Text] [Subscribe] [Scholar Register] [Indexed: 05/12/2025]
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7
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Sarkar S, Roy A, Mitra R, Kundu S, Banerjee P, Acharya Chowdhury A, Ghosh S. Escaping the ESKAPE pathogens: A review on antibiofilm potential of nanoparticles. Microb Pathog 2024; 194:106842. [PMID: 39117012 DOI: 10.1016/j.micpath.2024.106842] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 04/18/2024] [Revised: 07/31/2024] [Accepted: 08/05/2024] [Indexed: 08/10/2024]
Abstract
ESKAPE pathogens, a notorious consortium comprising Enterococcusfaecium, Staphylococcusaureus, Klebsiellapneumoniae, Acinetobacterbaumannii, Pseudomonasaeruginosa, and Enterobacter species, pose formidable challenges in healthcare settings due to their multidrug-resistant nature. The increasing global cases of antimicrobial-resistant ESKAPE pathogens are closely related to their remarkable ability to form biofilms. Thus, understanding the unique mechanisms of antimicrobial resistance of ESKAPE pathogens and the innate resilience of biofilms against traditional antimicrobial agents is important for developing innovative strategies to establish effective control methods against them. This review offers a thorough analysis of biofilm dynamics, with a focus on the general mechanisms of biofilm formation, the significant contribution of persister cells in the resistance mechanisms, and the recurrence of biofilms in comparison to planktonic cells. Additionally, this review highlights the potential strategies of nanoparticles for managing biofilms in the ESKAPE group of pathogens. Nanoparticles, with their unique physicochemical properties, provide promising opportunities for disrupting biofilm structures and improving antimicrobial effectiveness. The review has explored interactions between nanoparticles and biofilms, covering a range of nanoparticle types such as metal, metal-oxide, surface-modified, and functionalized nanoparticles, along with organic nanoparticles and nanomaterials. The additional focus of this review also encompasses green synthesis techniques of nanoparticles that involve plant extract and supernatants from bacterial and fungal cultures as reducing agents. Furthermore, the use of nanocomposites and nano emulsions in biofilm management of ESKAPE is also discussed. To conclude, the review addresses the current obstacles and future outlooks in nanoparticle-based biofilm management, stressing the necessity for further research and development to fully exploit the potential of nanoparticles in addressing biofilm-related challenges.
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Affiliation(s)
| | - Ankita Roy
- Department of Biosciences, JIS University, Kolkata, India
| | - Rangan Mitra
- Department of Biosciences, JIS University, Kolkata, India
| | - Sweta Kundu
- Department of Biosciences, JIS University, Kolkata, India
| | | | | | - Suparna Ghosh
- Department of Biosciences, JIS University, Kolkata, India.
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8
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Mishra S, Yadav MD. Magnetic Nanoparticles: A Comprehensive Review from Synthesis to Biomedical Frontiers. LANGMUIR : THE ACS JOURNAL OF SURFACES AND COLLOIDS 2024; 40:17239-17269. [PMID: 39132737 DOI: 10.1021/acs.langmuir.4c01532] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [MESH Headings] [Track Full Text] [Subscribe] [Scholar Register] [Indexed: 08/13/2024]
Abstract
Nanotechnology has opened new doors of exploration, particularly in materials science and healthcare. Magnetic nanoparticles (MNP), the tiny magnets, because of their various properties, have the potential to bring about radical changes in the field of medicine. The distinctive surface chemistry, nontoxicity, biocompatibility, and, in particular, the inducible magnetic moment of magnetic materials has attracted a great deal of interest in morphological structures from a variety of scientific domains. This review presents a concise overview of MNPs and their crucial properties and synthesis routes. It also aims to highlight the continuous synthesis methods available for MNP production. In recent years, the use of computational methods for understanding the behavior of nanoparticles has been on the rise. Thus, we also discuss the numerical models developed to understand how magnetic nanoparticles can be used in magnetic hyperthermia and targeting the Circle of Wilis. With the increasing use of MNPs in biomedical applications, it becomes necessary to understand the mechanisms of toxicity, which are elucidated in this review. The review focuses on the biomedical applications of MNPs in drug delivery, theranostics, and MRI contrasting agents. We anticipate that this article will broaden the perspective on magnetic nanoparticles and help to understand their functionality and applicability better.
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Affiliation(s)
- Shlok Mishra
- Department of Chemical Engineering, Institute of Chemical Technology, Mumbai 400019, India
| | - Manishkumar D Yadav
- Department of Chemical Engineering, Institute of Chemical Technology, Mumbai 400019, India
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9
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Bae E, Kim S, Sung JH, Kim JH, Jung SH, Song KS, Cho WS. The oxidative stress-dependent pulmonary inflammation of inhalable multi-walled carbon nanotube-containing nano-concrete dust and its comparison with conventional concrete dust and DQ12. JOURNAL OF HAZARDOUS MATERIALS 2024; 476:135214. [PMID: 39029181 DOI: 10.1016/j.jhazmat.2024.135214] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Subscribe] [Scholar Register] [Received: 02/26/2024] [Revised: 06/26/2024] [Accepted: 07/13/2024] [Indexed: 07/21/2024]
Abstract
Nano-concrete, which is an admixture of nanomaterials in concrete recipes, has been investigated to overcome the limitations of existing concrete, such as its stability and strength. However, there is no information on the human health effects of broken-down dust released during the construction and demolition efforts. In this study, we prepared an inhalable fraction of multi-walled carbon nanotube-containing nano-concrete dust and performed comparative toxicity studies with conventional concrete dust and DQ12 using a rat intratracheal instillation model. Although the recipes for concrete and nano-concrete are entirely different, the pulverized dust samples showed similar physicochemical properties, such as 0.46-0.48 µm diameter and chemical composition. Both concrete and nano-concrete dust exhibited similar patterns and magnitudes, representing acute neutrophilic inflammation and chronic active inflammation with lymphocyte infiltration. The toxicity endpoints of the tested particles at both time points showed an excellent correlation with the reactive oxygen species levels released from the alveolar macrophages, highlighting that alveolar macrophages are the primary target cells and that the oxidative stress paradigm is the main toxicity mechanism of the tested particles. In addition, the toxicity potentials of both concrete and nano-concrete dust were more than 10 times lower than that of DQ12.
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Affiliation(s)
- Eunsol Bae
- Lab of Toxicology, Department of Health Sciences, The Graduate School of Dong-A University, Busan 49315, Republic of Korea
| | - Songyeon Kim
- Lab of Toxicology, Department of Health Sciences, The Graduate School of Dong-A University, Busan 49315, Republic of Korea
| | - Jae Hyuck Sung
- Bio Division, Korea Conformity Laboratories, Incheon 21999, Republic of Korea
| | - Joo Hyung Kim
- Construction Division, Korea Conformity Laboratories, Cheongju 28115, Republic of Korea
| | - Sang Hwa Jung
- Construction Division, Korea Conformity Laboratories, Cheongju 28115, Republic of Korea
| | - Kyung-Seuk Song
- Bio Division, Korea Conformity Laboratories, Incheon 21999, Republic of Korea
| | - Wan-Seob Cho
- Lab of Toxicology, Department of Health Sciences, The Graduate School of Dong-A University, Busan 49315, Republic of Korea.
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10
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Francis AP, Meenakshi DU, Ganapathy S, Devasena T. Evaluating the ameliorative effect of nano bis-demethoxy curcumin analog against extrapulmonary toxicity in rat induced by inhaled multi-walled carbon nanotube. ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH INTERNATIONAL 2024; 31:46641-46651. [PMID: 37710065 DOI: 10.1007/s11356-023-29749-2] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Subscribe] [Scholar Register] [Received: 02/22/2023] [Accepted: 09/03/2023] [Indexed: 09/16/2023]
Abstract
Carbon nanotubes (CNTs) exposure in human beings through inhalation may affect pulmonary organs and extrapulmonary organs including liver, kidney, brain, spleen, etc. The toxic effects developed as the result of CNTs exposure made us to explore the beneficial effect of nano bis-demethoxy curcumin analog (NBDMCA) towards multi-walled carbon nanotubes (MWCNTs)-induced toxicity in extrapulmonary organs. The current study described the ameliorative effect of NBDMCA against the toxic effects developed by inhaled MWCNTs in the extrapulmonary organs. The rats are exposed to the fixed aerosol concentration of 5 mg/m3 maintained in inhalation exposure chambers MWCNTs for 15 days as per OECD guidelines. After the exposure with MWCNTs, the animals were treated with NBDMCA (5 mg/kg body weight) with different dose frequencies, i.e., 2 doses per week for 1, 2, and 4 weeks. After treatment duration, the blood was drawn from retro-orbital vein and subjected to biochemical and cytokine analysis. Further the animals were euthanized, and the sample tissues were collected and performed oxidative stress and histopathology. The study results revealed that the intravenous administration of NBDMCA suppresses the extrapulmonary toxicity induced by MWCNTs, i.e., annulling the clinical changes and oxidative stress in various extrapulmonary organs at low doses of NBDMCA, evidenced its antioxidant efficacy. Moreover, use of increased doses provides better reduction in toxic symptoms with negligible side effects confirming the dose-dependent efficacy of NBDMCA. Overall, we suggested that NBDMCA may materialize into an effective compound for the reduction of MWCNTs-induced toxicity.
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Affiliation(s)
- Arul Prakash Francis
- Centre of Molecular Medicine and Diagnostics (COMManD), Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai, 600 077, India
| | | | - Selvam Ganapathy
- International Institute of Biotechnology and Toxicology (IBAT), Padappai, 601301, India
| | - Thiyagarajan Devasena
- Centre for Nanoscience and Technology, A.C. Tech Campus, Anna University, Chennai, 600025, India.
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11
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Das SK, Sen K, Ghosh B, Ghosh N, Sinha K, Sil PC. Molecular mechanism of nanomaterials induced liver injury: A review. World J Hepatol 2024; 16:566-600. [PMID: 38689743 PMCID: PMC11056894 DOI: 10.4254/wjh.v16.i4.566] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Journal Information] [Submit a Manuscript] [Subscribe] [Scholar Register] [Received: 12/28/2023] [Revised: 02/05/2024] [Accepted: 03/19/2024] [Indexed: 04/24/2024] Open
Abstract
The unique physicochemical properties inherent to nanoscale materials have unveiled numerous potential applications, spanning beyond the pharmaceutical and medical sectors into various consumer industries like food and cosmetics. Consequently, humans encounter nanomaterials through diverse exposure routes, giving rise to potential health considerations. Noteworthy among these materials are silica and specific metallic nanoparticles, extensively utilized in consumer products, which have garnered substantial attention due to their propensity to accumulate and induce adverse effects in the liver. This review paper aims to provide an exhaustive examination of the molecular mechanisms underpinning nanomaterial-induced hepatotoxicity, drawing insights from both in vitro and in vivo studies. Primarily, the most frequently observed manifestations of toxicity following the exposure of cells or animal models to various nanomaterials involve the initiation of oxidative stress and inflammation. Additionally, we delve into the existing in vitro models employed for evaluating the hepatotoxic effects of nanomaterials, emphasizing the persistent endeavors to advance and bolster the reliability of these models for nanotoxicology research.
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Affiliation(s)
- Sanjib Kumar Das
- Department of Zoology, Jhargram Raj College, Jhargram 721507, India
| | - Koushik Sen
- Department of Zoology, Jhargram Raj College, Jhargram 721507, India
| | - Biswatosh Ghosh
- Department of Zoology, Bidhannagar College, Kolkata 700064, India
| | - Nabanita Ghosh
- Department of Zoology, Maulana Azad College, Kolkata 700013, India
| | - Krishnendu Sinha
- Department of Zoology, Jhargram Raj College, Jhargram 721507, India.
| | - Parames C Sil
- Department of Molecular Medicine, Bose Institute, Calcutta 700054, India
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12
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Audira G, Lee JS, Vasquez RD, Roldan MJM, Lai YH, Hsiao CD. Assessments of carbon nanotubes toxicities in zebrafish larvae using multiple physiological and molecular endpoints. Chem Biol Interact 2024; 392:110925. [PMID: 38452846 DOI: 10.1016/j.cbi.2024.110925] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 07/31/2023] [Revised: 10/01/2023] [Accepted: 02/20/2024] [Indexed: 03/09/2024]
Abstract
In recent years, carbon nanotubes (CNTs) have become one of the most promising materials for the technology industry. However, due to the extensive usage of these materials, they may be released into the environment, and cause toxicities to the organism. Here, their acute toxicities in zebrafish embryos and larvae were evaluated by using various assessments that may provide us with a novel perspective on their effects on aquatic animals. Before conducting the toxicity assessments, the CNTs were characterized as multiwall carbon nanotubes (MWCNTs) functionalized with hydroxyl and carboxyl groups, which improved their solubility and dispersibility. Based on the results, abnormalities in zebrafish behaviors were observed in the exposed groups, indicated by a reduction in tail coiling frequency and alterations in the locomotion as the response toward photo and vibration stimuli that might be due to the disruption in the neuromodulatory system and the formation of reactive oxygen species (ROS) by MWCNTs. Next, based on the respiratory rate assay, exposed larvae consumed more oxygen, which may be due to the injuries in the larval gill by the MWCNTs. Finally, even though no irregularity was observed in the exposed larval cardiac rhythm, abnormalities were shown in their cardiac physiology and blood flow with significant downregulation in several cardiac development-related gene expressions. To sum up, although the following studies are necessary to understand the exact mechanism of their toxicity, the current study demonstrated the environmental implications of MWCNTs in particularly low concentrations and short-term exposure, especially to aquatic organisms.
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Affiliation(s)
- Gilbert Audira
- Department of Bioscience Technology, Chung Yuan Christian University, Chung-Li, 320314, Taiwan; Department of Chemistry, Chung Yuan Christian University, Chung-Li, 320314, Taiwan
| | - Jiann-Shing Lee
- Department of Applied Physics, National Pingtung University, Pingtung, 900391, Taiwan
| | - Ross D Vasquez
- Department of Pharmacy, Faculty of Pharmacy, University of Santo Tomas, Manila, 1015, Philippines; Research Center for the Natural and Applied Sciences, University of Santo Tomas, Manila, 1015, Philippines; The Graduate School, University of Santo Tomas, Manila, 1015, Philippines
| | - Marri Jmelou M Roldan
- Faculty of Pharmacy, The Graduate School, University of Santo Tomas, Espana Blvd., Manila, 1015, Philippines
| | - Yu-Heng Lai
- Department of Chemistry, Chinese Culture University, Taipei, 11114, Taiwan
| | - Chung-Der Hsiao
- Department of Bioscience Technology, Chung Yuan Christian University, Chung-Li, 320314, Taiwan; Department of Chemistry, Chung Yuan Christian University, Chung-Li, 320314, Taiwan; Center of Nanotechnology, Chung Yuan Christian University, Chung-Li, 320314, Taiwan; Center for Aquatic Toxicology and Pharmacology, Chung Yuan Christian University, Chung-Li, 320314, Taiwan.
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13
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Nasim I, Ghani N, Nawaz R, Irfan A, Arshad M, Nasim M, Raish M, Irshad MA, Ghumman SA, Ahmad A, Bin Jardan YA. Investigating the Impact of Carbon Nanotube Nanoparticle Exposure on Testicular Oxidative Stress and Histopathological Changes in Swiss albino Mice. ACS OMEGA 2024; 9:6731-6740. [PMID: 38371818 PMCID: PMC10870293 DOI: 10.1021/acsomega.3c07919] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Grants] [Track Full Text] [Download PDF] [Figures] [Subscribe] [Scholar Register] [Received: 10/10/2023] [Revised: 01/12/2024] [Accepted: 01/18/2024] [Indexed: 02/20/2024]
Abstract
Carbon nanotubes (CNTs) possess remarkable properties that make them valuable for various industrial applications. However, concerns have arisen regarding their potential adverse health effects, particularly in occupational settings. The main aim of this research was to examine the effects of short-term exposure to multiwalled carbon nanotube nanoparticles (MWCNT-NPs) on testicular oxidative stress in Swiss albino mice, taking into account various factors such as dosage, duration of exposure, and particle size of MWCNT-NP. In this study, 20 mice were used and placed into six different groups randomly. Four of these groups comprised four repetitions each, while the two groups served as the vehicle control with two repetitions each. The experimental groups received MWCNT-NP treatment, whereas the control group remained untreated. The mice in the experimental groups were exposed to MWCNT-NP for either 7 days or 14 days. Through oral administration, the MWCNT-NP solution was introduced at two distinct dosages: 0.45 and 0.90 μg, whereas the control group was subjected to distilled water rather than the MWCNT-NP solution. The investigation evaluated primary oxidative balance indicators-glutathione (GSH) and glutathione disulfide (GSSG)-in response to MWCNT-NP exposure. Significantly, a noticeable reduction in GSH levels and a concurrent increase in GSSG concentrations were observed in comparison to the control group. To better understand and explore the assessment of the redox status, the Nernst equation was used to calculate the redox potential. Intriguingly, the calculated redox potential exhibited a negative value, signifying an imbalance in the oxidative state in the testes. These findings suggest that short-term exposure to MWCNT-NP can lead to the initiation of testicular oxidative stress and may disrupt the male reproductive system. This is evident from the alterations observed in the levels of GSH and GSSG, as well as the negative redox potential. The research offers significant insights into the reproductive effects of exposure to MWCNTs and emphasizes the necessity of assessing oxidative stress in nanomaterial toxicity studies.
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Affiliation(s)
- Iqra Nasim
- Department
of Environmental Science, Lahore College
for Women University, Lahore 54000, Pakistan
- Department
of Environmental Sciences, The University
of Lahore, Lahore 54000, Pakistan
| | - Nadia Ghani
- Department
of Environmental Science, Lahore College
for Women University, Lahore 54000, Pakistan
| | - Rab Nawaz
- Department
of Environmental Sciences, The University
of Lahore, Lahore 54000, Pakistan
- Faculty
of Engineering and Quantity Surveying, INTI
International University, Nilai 71800, Negeri Sembilan, Malaysia
| | - Ali Irfan
- Department
of Chemistry, Government College University
Faisalabad, Faisalabad 38000, Pakistan
| | - Muhammad Arshad
- Department
of Agriculture and Food Technology, Karakoram
International University, Gilgit 15100, Pakistan
| | - Maryam Nasim
- Institute
of Biomedical and Allied Health Sciences, University of Health Sciences, Lahore 54000, Pakistan
- Department
of Allied Health Sciences, Riphah International
University, Islamabad 46000, Pakistan
| | - Mohammad Raish
- Department
of Pharmaceutics, College of Pharmacy, King
Saud University, Riyadh 11451, Saudi Arabia
| | - Muhammad Atif Irshad
- Department
of Environmental Sciences, The University
of Lahore, Lahore 54000, Pakistan
| | | | - Ajaz Ahmad
- Department
of Clinical Pharmacy, College of Pharmacy, King Saud University, Riyadh 11451, Saudi Arabia
| | - Yousef A. Bin Jardan
- Department
of Pharmaceutics, College of Pharmacy, King
Saud University, Riyadh 11451, Saudi Arabia
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14
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Mohammed AN, Yadav N, Kaur P, Jandarov R, Yadav JS. Immunomodulation of susceptibility to pneumococcal pneumonia infection in mouse lungs exposed to carbon nanoparticles via dysregulation of innate and adaptive immune responses. Toxicol Appl Pharmacol 2024; 483:116820. [PMID: 38218205 DOI: 10.1016/j.taap.2024.116820] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 10/23/2023] [Revised: 01/05/2024] [Accepted: 01/10/2024] [Indexed: 01/15/2024]
Abstract
Carbon nanotubes (CNTs) are emerging pollutants of occupational and environmental health concern. While toxicological mechanisms of CNTs are emerging, there is paucity of information on their modulatory effects on susceptibility to infections. Here, we investigated cellular and molecular events underlying the effect of multi-walled CNT (MWCNT) exposure on susceptibility to Streptococcus pneumoniae infection in our 28-day sub-chronic exposure mouse model. Data indicated reduced phagocytic function in alveolar macrophages (AMs) from MWCNT-exposed lungs evidenced by lower pathogen uptake in 1-h infection assay. At 24-h post-infection, intracellular pathogen count in exposed AMs showed 2.5 times higher net increase (2-fold in vehicle- versus 5-fold in MWCNT-treated), indicating a greater rate of intracellular multiplication and/or survival due to MWCNT exposure. AMs from MWCNT-exposed lungs exhibited downregulation of pathogen-uptake receptors CD163, Phosphatidyl-serine receptor (Ptdsr), and Macrophage scavenger receptors class A type 1 (Msr1) and type 2 (MSr2). In whole lung, MWCNT exposure shifted the macrophage polarization state towards the immunosuppressive phenotype M2b and increased the CD11c+ dendritic cell population required to activate the adaptive immune response. Notably, the MWCNT pre-exposure dysregulated T-cell immunity, evidenced by diminished CD4 and Th17 response, and exacerbated Th1 and Treg responses (skewed Th17/Treg ratio), thereby favoring the pneumococcal infection. Overall, these findings indicated that MWCNT exposure compromises both innate and adaptive immunity leading to diminished host lung defense against pneumonia infection. To our knowledge, this is the first report on an immunomodulatory role of CNT pre-exposure on pneumococcal infection susceptibility due to dysregulation of both innate and adaptive immunity targets.
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Affiliation(s)
- Afzaal Nadeem Mohammed
- Pulmonary Pathogenesis and Immunotoxicology Laboratory, Division of Environmental Genetics and Molecular Toxicology, Department of Environmental and Public Health Sciences, University of Cincinnati College of Medicine, Cincinnati, OH 45267, USA
| | - Niket Yadav
- Medical Scientist Training Program, University of Virginia School of Medicine, Charlottesville, VA 22908-0738, USA
| | - Perminder Kaur
- Pulmonary Pathogenesis and Immunotoxicology Laboratory, Division of Environmental Genetics and Molecular Toxicology, Department of Environmental and Public Health Sciences, University of Cincinnati College of Medicine, Cincinnati, OH 45267, USA
| | - Roman Jandarov
- Division of Biostatistics and Bioinformatics, Department of Environmental and Public Health Sciences, University of Cincinnati College of Medicine, Cincinnati, OH 45267, USA
| | - Jagjit Singh Yadav
- Pulmonary Pathogenesis and Immunotoxicology Laboratory, Division of Environmental Genetics and Molecular Toxicology, Department of Environmental and Public Health Sciences, University of Cincinnati College of Medicine, Cincinnati, OH 45267, USA.
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15
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Moral R, Paul S. Exploring Cyclic Peptide Nanotube Stability Across Diverse Lipid Bilayers and Unveiling Water Transport Dynamics. LANGMUIR : THE ACS JOURNAL OF SURFACES AND COLLOIDS 2024; 40:882-895. [PMID: 38134046 DOI: 10.1021/acs.langmuir.3c03030] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [MESH Headings] [Track Full Text] [Subscribe] [Scholar Register] [Indexed: 12/24/2023]
Abstract
Cyclic Peptide Nanotubes (CPNTs) have emerged as compelling candidates for various applications, particularly as nanochannels within lipid bilayers. In this study, the stability of two CPNTs, namely 8 × [(Cys-Gly-Met-Gly)2] and 8 × [(Gly-Leu)4], are comprehensively investigated across different lipid bilayers, including 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC), a mixed model membrane (POPE/POPG), and a realistic yeast model membrane. The results demonstrate that both CPNTs maintain their tubular structures in all lipid bilayers, with [(Cys-Gly-Met-Gly)2] showing increased stability over an extended period in these lipid membranes. The insertion of CPNTs shows negligible impact on lipid bilayer properties, including area per lipid, volume per lipid, and bilayer thickness. The study demonstrates that the CPNT preserves its two-line water movement pattern within all the lipid membranes, reaffirming their potential as water channels. The MSD curves further reveal that the dynamics of water molecules inside the nanotube are similar for all the bilayer systems with minor differences that arise due to different lipid environments.
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Affiliation(s)
- Rimjhim Moral
- Department of Chemistry, Indian Institute of Technology, Guwahati, Assam 781039, India
| | - Sandip Paul
- Department of Chemistry, Indian Institute of Technology, Guwahati, Assam 781039, India
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16
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Dominguez J, Holmes SK, Bartone RD, Tisch LJ, Tighe RM, Bonner JC, Payne CK. House Dust Mite Extract Forms a Der p 2 Corona on Multi-Walled Carbon Nanotubes: Implications for Allergic Airway Disease. ENVIRONMENTAL SCIENCE. NANO 2024; 11:324-335. [PMID: 38577066 PMCID: PMC10990074 DOI: 10.1039/d3en00666b] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Grants] [Track Full Text] [Subscribe] [Scholar Register] [Indexed: 04/06/2024]
Abstract
Multi-walled carbons nanotubes (MWCNTs) are used in materials for the construction, automotive, and aerospace industries. Workers and consumers are exposed to these materials via inhalation. Existing recommended exposure limits are based on MWCNT exposures that do not take into account more realistic co-exposures. Our goal was to understand how a common allergen, house dust mites, interacts with pristine MWCNTs and lung fluid proteins. We used gel electrophoresis, western blotting, and proteomics to characterize the composition of the allergen corona formed from house dust mite extract on the surface of MWCNTs. We found that the corona is dominated by der p 2, a protein associated with human allergic responses to house dust mites. Der p 2 remains adsorbed on the surface of the MWCNTs following subsequent exposures to lung fluid proteins. The high concentration of der p 2, localized on surface of MWCNTs, has important implications for house dust mite-induced allergies and asthma. This research provides a detailed characterization of the complex house dust mite-lung fluid protein coronas for future cellular and in vivo studies. These studies will help to address the molecular and biochemical mechanisms underlying the exacerbation of allergic lung disease by nanomaterials.
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Affiliation(s)
- Judith Dominguez
- Thomas Lord Department of Mechanical Engineering and Materials Science, Duke University, Durham, North Carolina, USA 27708
| | - Samantha K. Holmes
- Thomas Lord Department of Mechanical Engineering and Materials Science, Duke University, Durham, North Carolina, USA 27708
| | - Ryan D. Bartone
- Toxicology Program, Department of Biological Sciences, North Carolina State University, Raleigh, North Carolina, USA 27695
| | - Logan J. Tisch
- Toxicology Program, Department of Biological Sciences, North Carolina State University, Raleigh, North Carolina, USA 27695
| | - Robert M. Tighe
- Department of Medicine, Duke University School of Medicine, Durham, North Carolina, USA 27710
| | - James C. Bonner
- Toxicology Program, Department of Biological Sciences, North Carolina State University, Raleigh, North Carolina, USA 27695
| | - Christine K. Payne
- Thomas Lord Department of Mechanical Engineering and Materials Science, Duke University, Durham, North Carolina, USA 27708
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17
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Mehrotra S, Dey S, Sachdeva K, Mohanty S, Mandal BB. Recent advances in tailoring stimuli-responsive hybrid scaffolds for cardiac tissue engineering and allied applications. J Mater Chem B 2023; 11:10297-10331. [PMID: 37905467 DOI: 10.1039/d3tb00450c] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 11/02/2023]
Abstract
To recapitulate bio-physical properties and functional behaviour of native heart tissues, recent tissue engineering-based approaches are focused on developing smart/stimuli-responsive materials for interfacing cardiac cells. Overcoming the drawbacks of the traditionally used biomaterials, these smart materials portray outstanding mechanical and conductive properties while promoting cell-cell interaction and cell-matrix transduction cues in such excitable tissues. To date, a large number of stimuli-responsive materials have been employed for interfacing cardiac tissues alone or in combination with natural/synthetic materials for cardiac tissue engineering. However, their comprehensive classification and a comparative analysis of the role played by these materials in regulating cardiac cell behaviour and in vivo metabolism are much less discussed. In an attempt to cover the recent advances in fabricating stimuli-responsive biomaterials for engineering cardiac tissues, this review details the role of these materials in modulating cardiomyocyte behaviour, functionality and surrounding matrix properties. Furthermore, concerns and challenges regarding the clinical translation of these materials and the possibility of using such materials for the fabrication of bio-actuators and bioelectronic devices are discussed.
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Affiliation(s)
- Shreya Mehrotra
- Department of Biosciences and Bioengineering, Indian Institute of Technology Guwahati, Guwahti-781039, Assam, India. biman.mandal@iitg,ac.in
| | - Souradeep Dey
- Centre for Nanotechnology, Indian Institute of Technology Guwahati, Guwahti-781039, Assam, India
| | - Kunj Sachdeva
- DBT-Centre of Excellence for Stem Cell Research, All India Institute of Medical Sciences, New Delhi-110029, India
| | - Sujata Mohanty
- DBT-Centre of Excellence for Stem Cell Research, All India Institute of Medical Sciences, New Delhi-110029, India
| | - Biman B Mandal
- Department of Biosciences and Bioengineering, Indian Institute of Technology Guwahati, Guwahti-781039, Assam, India. biman.mandal@iitg,ac.in
- Centre for Nanotechnology, Indian Institute of Technology Guwahati, Guwahti-781039, Assam, India
- Jyoti and Bhupat Mehta School of Health Sciences and Technology, Indian Institute of Technology Guwahati, Guwahati-781039, Assam, India
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18
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Ali MK, Javaid S, Afzal H, Zafar I, Fayyaz K, Ain Q, Rather MA, Hossain MJ, Rashid S, Khan KA, Sharma R. Exploring the multifunctional roles of quantum dots for unlocking the future of biology and medicine. ENVIRONMENTAL RESEARCH 2023; 232:116290. [PMID: 37295589 DOI: 10.1016/j.envres.2023.116290] [Citation(s) in RCA: 14] [Impact Index Per Article: 7.0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Subscribe] [Scholar Register] [Received: 03/24/2023] [Revised: 05/28/2023] [Accepted: 05/30/2023] [Indexed: 06/12/2023]
Abstract
With recent advancements in nanomedicines and their associated research with biological fields, their translation into clinically-applicable products is still below promises. Quantum dots (QDs) have received immense research attention and investment in the four decades since their discovery. We explored the extensive biomedical applications of QDs, viz. Bio-imaging, drug research, drug delivery, immune assays, biosensors, gene therapy, diagnostics, their toxic effects, and bio-compatibility. We unravelled the possibility of using emerging data-driven methodologies (bigdata, artificial intelligence, machine learning, high-throughput experimentation, computational automation) as excellent sources for time, space, and complexity optimization. We also discussed ongoing clinical trials, related challenges, and the technical aspects that should be considered to improve the clinical fate of QDs and promising future research directions.
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Affiliation(s)
- Muhammad Kashif Ali
- Deparment of Physiology, Rashid Latif Medical College, Lahore, Punjab, 54700, Pakistan.
| | - Saher Javaid
- KAM School of Life Sciences, Forman Christian College (a Chartered University) Lahore, Punjab, Pakistan.
| | - Haseeb Afzal
- Department of ENT, Ameer Ud Din Medical College, Lahore, Punjab, 54700, Pakistan.
| | - Imran Zafar
- Department of Bioinformatics and Computational Biology, Virtual University, Punjab, 54700, Pakistan.
| | - Kompal Fayyaz
- Department of National Centre for Bioinformatics, Quaid-I-Azam University, Islamabad, 45320, Pakistan.
| | - Quratul Ain
- Department of Chemistry, Government College Women University Faisalabad (GCWUF), Punjab, 54700, Pakistan.
| | - Mohd Ashraf Rather
- Division of Fish Genetics and Biotechnology, Faculty of Fisheries, Rangil- Gandarbal (SKAUST-K), India.
| | - Md Jamal Hossain
- Department of Pharmacy, State University of Bangladesh, 77 Satmasjid Road, Dhanmondi, Dhaka, 1205, Bangladesh.
| | - Summya Rashid
- Department of Pharmacology & Toxicology, College of Pharmacy, Prince Sattam Bin Abdulaziz University, P.O. Box 173, Al-Kharj, 11942, Saudi Arabia.
| | - Khalid Ali Khan
- Unit of Bee Research and Honey Production, Research Center for Advanced Materials Science (RCAMS), King Khalid University, P.O. Box 9004, Abha, 61413, Saudi Arabia; Applied College, King Khalid University, P. O. Box 9004, Abha, 61413, Saudi Arabia.
| | - Rohit Sharma
- Department of Rasa Shastra and Bhaishajya Kalpana, Faculty of Ayurveda, Institute of Medical Sciences, Banaras Hindu University, Varanasi, 221005, Uttar Pradesh, India.
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19
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Parikh SD, Wang W, Nelson MT, Sulentic CEW, Mukhopadhyay SM. Bioinspired Hierarchical Carbon Structures as Potential Scaffolds for Wound Healing and Tissue Regeneration Applications. NANOMATERIALS (BASEL, SWITZERLAND) 2023; 13:nano13111791. [PMID: 37299693 DOI: 10.3390/nano13111791] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Grants] [Track Full Text] [Subscribe] [Scholar Register] [Received: 04/19/2023] [Revised: 05/22/2023] [Accepted: 05/29/2023] [Indexed: 06/12/2023]
Abstract
Engineered bio-scaffolds for wound healing provide an attractive treatment option for tissue engineering and traumatic skin injuries since they can reduce dependence on donors and promote faster repair through strategic surface engineering. Current scaffolds present limitations in handling, preparation, shelf life, and sterilization options. In this study, bio-inspired hierarchical all-carbon structures comprising carbon nanotube (CNT) carpets covalently bonded to flexible carbon fabric have been investigated as a platform for cell growth and future tissue regeneration applications. CNTs are known to provide guidance for cell growth, but loose CNTs are susceptible to intracellular uptake and are suspected to cause in vitro and in vivo cytotoxicity. This risk is suppressed in these materials due to the covalent attachment of CNTs on a larger fabric, and the synergistic benefits of nanoscale and micro-macro scale architectures, as seen in natural biological materials, can be obtained. The structural durability, biocompatibility, tunable surface architecture, and ultra-high specific surface area of these materials make them attractive candidates for wound healing. In this study, investigations of cytotoxicity, skin cell proliferation, and cell migration were performed, and results indicate promise in both biocompatibility and directed cell growth. Moreover, these scaffolds provided cytoprotection against environmental stressors such as Ultraviolet B (UVB) rays. It was seen that cell growth could also be tailored through the control of CNT carpet height and surface wettability. These results support future promise in the design of hierarchical carbon scaffolds for strategic wound healing and tissue regeneration applications.
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Affiliation(s)
- Soham D Parikh
- Department of Mechanical & Materials Engineering, Wright State University, 3640 Col. Glen Hwy, Dayton, OH 45435, USA
| | - Wenhu Wang
- Frontier Institute for Research in Sensor Technologies (FIRST), University of Maine, United States Air Force Research Laboratory, Orono, ME 04469, USA
| | - M Tyler Nelson
- 711th Human Performance Wing, Airman Systems Directorate, Bioengineering Division, Wright-Patterson Air Force Base, Dayton, OH 45433, USA
| | - Courtney E W Sulentic
- Department of Pharmacology and Toxicology, Wright State University, Boonshoft School of Medicine, 3640 Col. Glen Hwy, Dayton, OH 45435, USA
| | - Sharmila M Mukhopadhyay
- Frontier Institute for Research in Sensor Technologies (FIRST), University of Maine, United States Air Force Research Laboratory, Orono, ME 04469, USA
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20
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Lim CS, Veltri B, Kashon M, Porter DW, Ma Q. Multi-walled carbon nanotubes induce arachidonate 5-lipoxygenase expression and enhance the polarization and function of M1 macrophages in vitro. Nanotoxicology 2023; 17:249-269. [PMID: 37115655 DOI: 10.1080/17435390.2023.2204161] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 04/29/2023]
Abstract
Fibrogenic carbon nanotubes (CNTs) induce the polarization of M1 and M2 macrophages in mouse lungs. Polarization of the macrophages regulates the production of proinflammatory and pro-resolving lipid mediators (LMs) to mediate acute inflammation and its resolution in a time-dependent manner. Here we examined the molecular mechanism by which multi-walled CNTs (MWCNTs, Mitsui-7) induce M1 polarization in vitro. Treatment of murine macrophages (J774A.1) with Mitsui-7 MWCNTs increased the expression of Alox5 mRNA and protein in a concentration- and time-dependent manner. The MWCNTs induced the expression of CD68 and that induction persisted for up to 3 days post-exposure. The expression and activity of inducible nitric oxide synthase, an intracellular marker of M1, were increased by MWCNTs. Consistent with M1 polarization, the MWCNTs induced the production and secretion of proinflammatory cytokines tumor necrosis factor-α and interleukin-1β, and proinflammatory LMs leukotriene B4 (LTB4) and prostaglandin E2 (PGE2). The cell-free media from MWCNT-polarized macrophages induced the migration of neutrophilic cells (differentiated from HL-60), which was blocked by Acebilustat, a specific leukotriene A4 hydrolase inhibitor, or LY239111, an LTB4 receptor antagonist, but not NS-398, a cyclooxygenase 2 inhibitor, revealing LTB4 as a major mediator of neutrophil chemotaxis from MWCNT-polarized macrophages. Knockdown of Alox5 using specific small hairpin-RNA suppressed MWCNT-induced M1 polarization, LTB4 secretion, and migration of neutrophils. Taken together, these findings demonstrate the polarization of M1 macrophages by Mitsui-7 MWCNTs in vitro and that induction of Alox5 is an important mechanism by which the MWCNTs promote proinflammatory responses by boosting M1 polarization and production of proinflammatory LMs.
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Affiliation(s)
- Chol Seung Lim
- Receptor Biology Laboratory, Toxicology and Molecular Biology Branch, Health Effects Laboratory Division, National Institute for Occupational Safety and Health, Centers for Disease Control and Prevention, Morgantown, WV, USA
| | - Brandon Veltri
- Department of Microbiology, Immunology, and Cell Biology, Robert C. Byrd Health Sciences Center, West Virginia University, Morgantown, WV, USA
| | - Michael Kashon
- Bioanalytics Branch, Health Effects Laboratory Division, National Institute for Occupational Safety and Health, Centers for Disease Control and Prevention, Morgantown, WV, USA
| | - Dale W Porter
- Pathology and Physiology Research Branch, Health Effects Laboratory Division, National Institute for Occupational Safety and Health, Centers for Disease Control and Prevention, Morgantown, WV, USA
| | - Qiang Ma
- Receptor Biology Laboratory, Toxicology and Molecular Biology Branch, Health Effects Laboratory Division, National Institute for Occupational Safety and Health, Centers for Disease Control and Prevention, Morgantown, WV, USA
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21
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Keshavan S, Bannuscher A, Drasler B, Barosova H, Petri-Fink A, Rothen-Rutishauser B. Comparing species-different responses in pulmonary fibrosis research: Current understanding of in vitro lung cell models and nanomaterials. Eur J Pharm Sci 2023; 183:106387. [PMID: 36652970 DOI: 10.1016/j.ejps.2023.106387] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 08/12/2022] [Revised: 12/16/2022] [Accepted: 01/14/2023] [Indexed: 01/16/2023]
Abstract
Pulmonary fibrosis (PF) is a chronic, irreversible lung disease that is typically fatal and characterized by an abnormal fibrotic response. As a result, vast areas of the lungs are gradually affected, and gas exchange is impaired, making it one of the world's leading causes of death. This can be attributed to a lack of understanding of the onset and progression of the disease, as well as a poor understanding of the mechanism of adverse responses to various factors, such as exposure to allergens, nanomaterials, environmental pollutants, etc. So far, the most frequently used preclinical evaluation paradigm for PF is still animal testing. Nonetheless, there is an urgent need to understand the factors that induce PF and find novel therapeutic targets for PF in humans. In this regard, robust and realistic in vitro fibrosis models are required to understand the mechanism of adverse responses. Over the years, several in vitro and ex vivo models have been developed with the goal of mimicking the biological barriers of the lung as closely as possible. This review summarizes recent progress towards the development of experimental models suitable for predicting fibrotic responses, with an emphasis on cell culture methods, nanomaterials, and a comparison of results from studies using cells from various species.
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Affiliation(s)
- Sandeep Keshavan
- Adolphe Merkle Institute, University of Fribourg, Chemin des Verdiers 4, Fribourg CH-1700, Switzerland
| | - Anne Bannuscher
- Adolphe Merkle Institute, University of Fribourg, Chemin des Verdiers 4, Fribourg CH-1700, Switzerland
| | - Barbara Drasler
- Adolphe Merkle Institute, University of Fribourg, Chemin des Verdiers 4, Fribourg CH-1700, Switzerland
| | - Hana Barosova
- Institute of Experimental Medicine of the Czech Academy of Sciences, Videnska 1083, Prague 14220, Czech Republic
| | - Alke Petri-Fink
- Adolphe Merkle Institute, University of Fribourg, Chemin des Verdiers 4, Fribourg CH-1700, Switzerland; Chemistry Department, University of Fribourg, Chemin du Musée 9, Fribourg 1700, Switzerland
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22
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Bin Rashid A. Utilization of Nanotechnology and Nanomaterials in Biodiesel Production and Property Enhancement. JOURNAL OF NANOMATERIALS 2023; 2023:1-14. [DOI: 10.1155/2023/7054045] [Citation(s) in RCA: 2] [Impact Index Per Article: 1.0] [Reference Citation Analysis] [Abstract] [Track Full Text] [Subscribe] [Scholar Register] [Indexed: 09/01/2023]
Abstract
In today’s world, the applications of nanotechnology and nanomaterials are attracting interest in a wide variety of study domains because of their appealing qualities. The use of nanotechnology and nanomaterials in biodiesel processing and manufacturing is a focus of research globally. For accelerating the progress and development of biodiesel production, more focus is being given to the application of advanced nanotechnology for maximum yield in low cost. Hence, this paper will discuss the utilization of numerous nanomaterials/nanocatalysts for biodiesel synthesis from multiple feedstocks. This study will also focus on nanomaterials’ applications in algae cultivation and lipid extraction. Furthermore, the current study will comprehensively overview the nanoadditives blended biodiesel in diesel engines and the significant challenges and future opportunities. Moreover, this paper will also focus on human and environmental safety concerns of nanotechnology-based large-scale biodiesel production. Hence, this review will provide perception for future manufacturers, researchers, and academicians into the extent of research in nanotechnology and nanomaterials assisted biodiesel production and its efficiency enhancement.
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Affiliation(s)
- Adib Bin Rashid
- Department of Industrial and Production Engineering, Military Institute of Science and Technology, Dhaka, Bangladesh
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23
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Kök M, Pekdemir ME, Özen Öner E, Coşkun M, Hekim S. MWCNT nanocomposite films prepared using different ratios of PVC/PCL: Combined FT-IR/DFT, thermal and shape memory properties. J Mol Struct 2023. [DOI: 10.1016/j.molstruc.2023.134989] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 01/20/2023]
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24
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Zhang G, Luo W, Yang W, Li S, Li D, Zeng Y, Li Y. The importance of the
IL
‐1 family of cytokines in nanoimmunosafety and nanotoxicology. WIRES NANOMEDICINE AND NANOBIOTECHNOLOGY 2022; 14:e1850. [DOI: 10.1002/wnan.1850] [Citation(s) in RCA: 1] [Impact Index Per Article: 0.3] [Reference Citation Analysis] [Track Full Text] [Subscribe] [Scholar Register] [Received: 04/03/2022] [Revised: 08/03/2022] [Accepted: 08/11/2022] [Indexed: 11/24/2022]
Affiliation(s)
- Guofang Zhang
- Laboratory of Immunology and Nanomedicine Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences Shenzhen China
| | - Wenhe Luo
- Laboratory of Immunology and Nanomedicine Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences Shenzhen China
| | - Wenjie Yang
- Laboratory of Immunology and Nanomedicine Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences Shenzhen China
| | - Su Li
- Laboratory of Immunology and Nanomedicine Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences Shenzhen China
| | - Dongjie Li
- Laboratory of Immunology and Nanomedicine Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences Shenzhen China
| | - Yanqiao Zeng
- Laboratory of Immunology and Nanomedicine Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences Shenzhen China
| | - Yang Li
- Laboratory of Immunology and Nanomedicine Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences Shenzhen China
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Bai X, Smith ZL, Wang Y, Butterworth S, Tirella A. Sustained Drug Release from Smart Nanoparticles in Cancer Therapy: A Comprehensive Review. MICROMACHINES 2022; 13:mi13101623. [PMID: 36295976 PMCID: PMC9611581 DOI: 10.3390/mi13101623] [Citation(s) in RCA: 36] [Impact Index Per Article: 12.0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Subscribe] [Scholar Register] [Received: 09/08/2022] [Revised: 09/22/2022] [Accepted: 09/22/2022] [Indexed: 05/14/2023]
Abstract
Although nanomedicine has been highly investigated for cancer treatment over the past decades, only a few nanomedicines are currently approved and in the market; making this field poorly represented in clinical applications. Key research gaps that require optimization to successfully translate the use of nanomedicines have been identified, but not addressed; among these, the lack of control of the release pattern of therapeutics is the most important. To solve these issues with currently used nanomedicines (e.g., burst release, systemic release), different strategies for the design and manufacturing of nanomedicines allowing for better control over the therapeutic release, are currently being investigated. The inclusion of stimuli-responsive properties and prolonged drug release have been identified as effective approaches to include in nanomedicine, and are discussed in this paper. Recently, smart sustained release nanoparticles have been successfully designed to safely and efficiently deliver therapeutics with different kinetic profiles, making them promising for many drug delivery applications and in specific for cancer treatment. In this review, the state-of-the-art of smart sustained release nanoparticles is discussed, focusing on the design strategies and performances of polymeric nanotechnologies. A complete list of nanomedicines currently tested in clinical trials and approved nanomedicines for cancer treatment is presented, critically discussing advantages and limitations with respect to the newly developed nanotechnologies and manufacturing methods. By the presented discussion and the highlight of nanomedicine design criteria and current limitations, this review paper could be of high interest to identify key features for the design of release-controlled nanomedicine for cancer treatment.
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Affiliation(s)
- Xue Bai
- Division of Pharmacy and Optometry, School of Health Science, Faculty of Biology, Medicine and Health, University of Manchester, Oxford Road, Manchester M13 9PT, UK
| | - Zara L. Smith
- Division of Pharmacy and Optometry, School of Health Science, Faculty of Biology, Medicine and Health, University of Manchester, Oxford Road, Manchester M13 9PT, UK
| | - Yuheng Wang
- Division of Pharmacy and Optometry, School of Health Science, Faculty of Biology, Medicine and Health, University of Manchester, Oxford Road, Manchester M13 9PT, UK
| | - Sam Butterworth
- Division of Pharmacy and Optometry, School of Health Science, Faculty of Biology, Medicine and Health, University of Manchester, Oxford Road, Manchester M13 9PT, UK
| | - Annalisa Tirella
- Division of Pharmacy and Optometry, School of Health Science, Faculty of Biology, Medicine and Health, University of Manchester, Oxford Road, Manchester M13 9PT, UK
- BIOtech-Center for Biomedical Technologies, Department of Industrial Engineering, University of Trento, Via delle Regole 101, 38123 Trento, Italy
- Correspondence:
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de Luna LAV, Loret T, Fordham A, Arshad A, Drummond M, Dodd A, Lozano N, Kostarelos K, Bussy C. Lung recovery from DNA damage induced by graphene oxide is dependent on size, dose and inflammation profile. Part Fibre Toxicol 2022; 19:62. [PMID: 36131347 PMCID: PMC9490925 DOI: 10.1186/s12989-022-00502-w] [Citation(s) in RCA: 10] [Impact Index Per Article: 3.3] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 05/30/2022] [Accepted: 09/06/2022] [Indexed: 11/10/2022] Open
Abstract
BACKGROUND A key aspect of any new material safety assessment is the evaluation of their in vivo genotoxicity. Graphene oxide (GO) has been studied for many promising applications, but there are remaining concerns about its safety profile, especially after inhalation. Herein we tested whether GO lateral dimension, comparing micrometric (LGO) and nanometric (USGO) GO sheets, has a role in the formation of DNA double strand breaks in mouse lungs. We used spatial resolution and differential cell type analysis to measure DNA damages in both epithelial and immune cells, after either single or repeated exposure. RESULTS GO induced DNA damages were size and dose dependent, in both exposure scenario. After single exposure to a high dose, both USGO and LGO induced significant DNA damage in the lung parenchyma, but only during the acute phase response (p < 0.05 for USGO; p < 0.01 for LGO). This was followed by a fast lung recovery at day 7 and 28 for both GOs. When evaluating the chronic impact of GO after repeated exposure, only a high dose of LGO induced long-term DNA damages in lung alveolar epithelia (at 84 days, p < 0.05). Regardless of size, low dose GO did not induce any significant DNA damage after repeated exposure. A multiparametric correlation analysis of our repeated exposure data revealed that transient or persistent inflammation and oxidative stress were associated to either recovery or persistent DNA damages. For USGO, recovery from DNA damage was correlated to efficient recovery from acute inflammation (i.e., significant secretion of SAA3, p < 0.001; infiltration of neutrophils, p < 0.01). In contrast, the persistence of LGO in lungs was associated to a long-lasting presence of multinucleated macrophages (up to 84 days, p < 0.05), an underlying inflammation (IL-1α secretion up to 28 days, p < 0.05) and the presence of persistent DNA damages at 84 days. CONCLUSIONS Overall these results highlight the importance of the exposure scenario used. We showed that LGO was more genotoxic after repeated exposure than single exposure due to persistent lung inflammation. These findings are important in the context of human health risk assessment and toward establishing recommendations for a safe use of graphene based materials in the workplace.
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Affiliation(s)
- Luis Augusto Visani de Luna
- Nanomedicine Lab 2.0, Faculty of Biology, Medicine and Health, Manchester Academic Health Science Centre, The University of Manchester, Manchester, M13 9PT, UK.,National Graphene Institute, The University of Manchester, Manchester, M13 9PL, UK.,Lydia Becker Institute of Immunology and Inflammation, Faculty of Biology, Medicine and Health, Manchester Academic Health Science Centre, The University of Manchester, Manchester, M13 9PT, UK
| | - Thomas Loret
- Nanomedicine Lab 2.0, Faculty of Biology, Medicine and Health, Manchester Academic Health Science Centre, The University of Manchester, Manchester, M13 9PT, UK.,National Graphene Institute, The University of Manchester, Manchester, M13 9PL, UK.,Lydia Becker Institute of Immunology and Inflammation, Faculty of Biology, Medicine and Health, Manchester Academic Health Science Centre, The University of Manchester, Manchester, M13 9PT, UK
| | - Alexander Fordham
- Nanomedicine Lab 2.0, Faculty of Biology, Medicine and Health, Manchester Academic Health Science Centre, The University of Manchester, Manchester, M13 9PT, UK.,National Graphene Institute, The University of Manchester, Manchester, M13 9PL, UK.,Lydia Becker Institute of Immunology and Inflammation, Faculty of Biology, Medicine and Health, Manchester Academic Health Science Centre, The University of Manchester, Manchester, M13 9PT, UK
| | - Atta Arshad
- Nanomedicine Lab 2.0, Faculty of Biology, Medicine and Health, Manchester Academic Health Science Centre, The University of Manchester, Manchester, M13 9PT, UK.,National Graphene Institute, The University of Manchester, Manchester, M13 9PL, UK.,Lydia Becker Institute of Immunology and Inflammation, Faculty of Biology, Medicine and Health, Manchester Academic Health Science Centre, The University of Manchester, Manchester, M13 9PT, UK
| | - Matthew Drummond
- Nanomedicine Lab 2.0, Faculty of Biology, Medicine and Health, Manchester Academic Health Science Centre, The University of Manchester, Manchester, M13 9PT, UK.,National Graphene Institute, The University of Manchester, Manchester, M13 9PL, UK
| | - Abbie Dodd
- Nanomedicine Lab 2.0, Faculty of Biology, Medicine and Health, Manchester Academic Health Science Centre, The University of Manchester, Manchester, M13 9PT, UK.,National Graphene Institute, The University of Manchester, Manchester, M13 9PL, UK
| | - Neus Lozano
- Catalan Institute of Nanoscience and Nanotechnology (ICN2), CSIC and BIST, Campus UAB, Bellaterra, 08193, Barcelona, Spain
| | - Kostas Kostarelos
- Nanomedicine Lab 2.0, Faculty of Biology, Medicine and Health, Manchester Academic Health Science Centre, The University of Manchester, Manchester, M13 9PT, UK.,National Graphene Institute, The University of Manchester, Manchester, M13 9PL, UK.,Catalan Institute of Nanoscience and Nanotechnology (ICN2), CSIC and BIST, Campus UAB, Bellaterra, 08193, Barcelona, Spain
| | - Cyrill Bussy
- Nanomedicine Lab 2.0, Faculty of Biology, Medicine and Health, Manchester Academic Health Science Centre, The University of Manchester, Manchester, M13 9PT, UK. .,National Graphene Institute, The University of Manchester, Manchester, M13 9PL, UK. .,Lydia Becker Institute of Immunology and Inflammation, Faculty of Biology, Medicine and Health, Manchester Academic Health Science Centre, The University of Manchester, Manchester, M13 9PT, UK.
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Mondal J, An JM, Surwase SS, Chakraborty K, Sutradhar SC, Hwang J, Lee J, Lee YK. Carbon Nanotube and Its Derived Nanomaterials Based High Performance Biosensing Platform. BIOSENSORS 2022; 12:731. [PMID: 36140116 PMCID: PMC9496036 DOI: 10.3390/bios12090731] [Citation(s) in RCA: 18] [Impact Index Per Article: 6.0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Grants] [Track Full Text] [Download PDF] [Figures] [Subscribe] [Scholar Register] [Received: 07/30/2022] [Revised: 08/25/2022] [Accepted: 08/30/2022] [Indexed: 11/30/2022]
Abstract
After the COVID-19 pandemic, the development of an accurate diagnosis and monitoring of diseases became a more important issue. In order to fabricate high-performance and sensitive biosensors, many researchers and scientists have used many kinds of nanomaterials such as metal nanoparticles (NPs), metal oxide NPs, quantum dots (QDs), and carbon nanomaterials including graphene and carbon nanotubes (CNTs). Among them, CNTs have been considered important biosensing channel candidates due to their excellent physical properties such as high electrical conductivity, strong mechanical properties, plasmonic properties, and so on. Thus, in this review, CNT-based biosensing systems are introduced and various sensing approaches such as electrochemical, optical, and electrical methods are reported. Moreover, such biosensing platforms showed excellent sensitivity and high selectivity against not only viruses but also virus DNA structures. So, based on the amazing potential of CNTs-based biosensing systems, healthcare and public health can be significantly improved.
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Affiliation(s)
- Jagannath Mondal
- Department of Green Bio Engineering, Korea National University of Transportation, Chungju 27469, Korea
| | - Jeong Man An
- Department of Bioengineering, College of Engineering, Hanyang University, Seoul 04763, Korea
| | - Sachin S. Surwase
- 4D Convergence Technology Institute, Korea National University of Transportation, Jungpyeong 27909, Korea
| | - Kushal Chakraborty
- Department of IT and Energy Convergence (BK21 FOUR), Korea National University of Transportation, Chungju 27469, Korea
| | - Sabuj Chandra Sutradhar
- 4D Convergence Technology Institute, Korea National University of Transportation, Jungpyeong 27909, Korea
| | - Joon Hwang
- 4D Convergence Technology Institute, Korea National University of Transportation, Jungpyeong 27909, Korea
- Department of Aeronautical & Mechanical Design Engineering, Korea National University of Transportation, Chungju 27469, Korea
| | - Jaewook Lee
- 4D Convergence Technology Institute, Korea National University of Transportation, Jungpyeong 27909, Korea
| | - Yong-Kyu Lee
- Department of Green Bio Engineering, Korea National University of Transportation, Chungju 27469, Korea
- 4D Convergence Technology Institute, Korea National University of Transportation, Jungpyeong 27909, Korea
- Department of Chemical and Biological Engineering, Korea National University of Transportation, Chungju 27469, Korea
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Sallam MF, Ahmed HMS, Diab KA, El-Nekeety AA, Abdel-Aziem SH, Sharaf HA, Abdel-Wahhab MA. Improvement of the antioxidant activity of thyme essential oil against biosynthesized titanium dioxide nanoparticles-induced oxidative stress, DNA damage, and disturbances in gene expression in vivo. J Trace Elem Med Biol 2022; 73:127024. [PMID: 35753172 DOI: 10.1016/j.jtemb.2022.127024] [Citation(s) in RCA: 13] [Impact Index Per Article: 4.3] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Journal Information] [Submit a Manuscript] [Subscribe] [Scholar Register] [Received: 03/09/2022] [Revised: 06/04/2022] [Accepted: 06/18/2022] [Indexed: 10/18/2022]
Abstract
BACKGROUND Titanium dioxide nanoparticles (TiO2-NPs) are widely utilized in medicine and industry; however, their safety in biological organisms is still unclear. In this study, we determined the bioactive constitutes of thyme essential oil (TEO) and utilized the nanoemulsion technique to improve its protective efficiency against oxidative stress, genotoxicity, and DNA damage of biosynthesized titanium dioxide nanoparticles (TiO2-NPs). METHODS TEO nanoemulsion (TEON) was prepared using whey protein isolate (WPI). Sixty male Sprague-Dawley rats were divided into six groups and treated orally for 21 days including the control group, TEO, or TEON- treated groups (5 mg/kg b.w), TiO2-NPs-treated group (50 mg/kg b.w) and the groups received TiO2-NPs plus TEO or TEON. Blood and tissues samples were collected for different assays. RESULTS The GC-MS analysis identified 17 bioactive compounds in TEO and thymol and carvacrol were the major compounds. TEON was irregular with average particles size of 230 ± 3.7 nm and ζ-potential of -24.17 mV. However, TiO2-NPs showed a polygonal shape with an average size of 50 ± 2.4 nm and ζ-potential of -30.44 mV. Animals that received TiO2-NPs showed severe disturbances in liver and kidney indices, lipid profile, oxidant/antioxidant indices, inflammatory cytokines, gene expressions, increased DNA damage, and pathological changes in hepatic tissue. Both TEO and TEON showed potential protection against these hazards and TEON was more effective than TEO. CONCLUSION The nanoemulsion of TEO enhances the oil bioactivity, improves its antioxidant characteristics, and protects against oxidative damage and genotoxicity of TiO2-NPs.
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Affiliation(s)
- Mohamed F Sallam
- Pharmacology and Toxicology Department, Faculty of Pharmacy, Ahram Canadian University, Giza, Egypt
| | - Helmy M S Ahmed
- Toxicology & Pharmacology Department, Faculty of Pharmacy, Cairo University, Cairo, Egypt.
| | - Kawther A Diab
- Genetics and Cytology Department, National Research Center, Dokki, Cairo, Egypt
| | - Aziza A El-Nekeety
- Food Toxicology & Contaminants Department, National Research Center, Dokki, Cairo, Egypt
| | | | - Hafiza A Sharaf
- Pathology Department, National Research Center, Dokki, Cairo, Egypt
| | - Mosaad A Abdel-Wahhab
- Food Toxicology & Contaminants Department, National Research Center, Dokki, Cairo, Egypt.
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Islam N, Saikia BK. An overview on atmospheric carbonaceous particulate matter into carbon nanomaterials: A new approach for air pollution mitigation. CHEMOSPHERE 2022; 303:135027. [PMID: 35623423 DOI: 10.1016/j.chemosphere.2022.135027] [Citation(s) in RCA: 7] [Impact Index Per Article: 2.3] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Subscribe] [Scholar Register] [Received: 02/26/2022] [Revised: 05/16/2022] [Accepted: 05/17/2022] [Indexed: 06/15/2023]
Abstract
Air pollutants consisting of atmospheric particulate matter (PM) poses a major threat to the environment and human health. However, due to their carbonaceous nature, these atmospheric PM can also be used as a precursor for fabrication of high-valued carbon nanomaterials (CNMs) leading to waste to wealth as well as mitigation of air pollution. Over the few years, various results have been reported on different types of physical and chemical methods for the synthesis of CNMs from atmospheric particulate matter with the help of top down and bottom up methods; however, there is a lack of review on these innovative processes and outcome in order to assess their feasibility and suitability for further investigation. This review critically assesses the synthesis, identification, and characterization of different types of CNMs derived from the atmospheric PM. The fascinating fluorescence properties along with the novel multifarious applications of such PM-derived CNMs are also extensively discussed in this review work. This unique review will certainly help to make a new avenue for air pollution mitigation through conversion of PMs in to value added nanomaterials (VNMs) and will boost the research activity in the field of environmental nanotechnology for a cleaner environment.
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Affiliation(s)
- Nazrul Islam
- Coal & Energy Division, CSIR-North East Institute of Science & Technology, Jorhat, 785006, India; Academy of Scientific & Innovative Research (AcSIR), Ghaziabad, 201002, India
| | - Binoy K Saikia
- Coal & Energy Division, CSIR-North East Institute of Science & Technology, Jorhat, 785006, India; Academy of Scientific & Innovative Research (AcSIR), Ghaziabad, 201002, India.
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Saito N, Haniu H, Aoki K, Nishimura N, Uemura T. Future Prospects for Clinical Applications of Nanocarbons Focusing on Carbon Nanotubes. ADVANCED SCIENCE (WEINHEIM, BADEN-WURTTEMBERG, GERMANY) 2022; 9:e2201214. [PMID: 35754236 PMCID: PMC9404397 DOI: 10.1002/advs.202201214] [Citation(s) in RCA: 1] [Impact Index Per Article: 0.3] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Figures] [Subscribe] [Scholar Register] [Received: 03/02/2022] [Revised: 05/18/2022] [Indexed: 06/15/2023]
Abstract
Over the past 15 years, numerous studies have been conducted on the use of nanocarbons as biomaterials towards such applications as drug delivery systems, cancer therapy, and regenerative medicine. However, the clinical use of nanocarbons remains elusive, primarily due to short- and long-term safety concerns. It is essential that the biosafety of each therapeutic modality be demonstrated in logical and well-conducted experiments. Accordingly, the fundamental techniques for assessing nanocarbon biomaterial safety have become more advanced. Optimal controls are being established, nanocarbon dispersal techniques are being refined, the array of biokinetic evaluation methods has increased, and carcinogenicity examinations under strict conditions have been developed. The medical implementation of nanocarbons as a biomaterial is in sight. With a particular focus on carbon nanotubes, these perspectives aim to summarize the contributions to date on nanocarbon applications and biosafety, introduce the recent achievements in evaluation techniques, and clarify the future prospects and systematic introduction of carbon nanomaterials for clinical use through practical yet sophisticated assessment methods.
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Affiliation(s)
- Naoto Saito
- Institute for Biomedical SciencesInterdisciplinary Cluster for Cutting Edge ResearchShinshu University3‐1‐1 AsahiMatsumotoNagano390‐8621Japan
| | - Hisao Haniu
- Institute for Biomedical SciencesInterdisciplinary Cluster for Cutting Edge ResearchShinshu University3‐1‐1 AsahiMatsumotoNagano390‐8621Japan
| | - Kaoru Aoki
- Department of Applied Physical TherapyShinshu University School of Health Sciences3‐1‐1 AsahiMatsumotoNagano390‐8621Japan
| | - Naoyuki Nishimura
- Institute for Biomedical SciencesInterdisciplinary Cluster for Cutting Edge ResearchShinshu University3‐1‐1 AsahiMatsumotoNagano390‐8621Japan
| | - Takeshi Uemura
- Institute for Biomedical SciencesInterdisciplinary Cluster for Cutting Edge ResearchShinshu University3‐1‐1 AsahiMatsumotoNagano390‐8621Japan
- Division of Gene ResearchResearch Center for Supports to Advanced ScienceShinshu University3‐1‐1 AsahiMatsumotoNagano390‐8621Japan
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Ampah JD, Yusuf AA, Agyekum EB, Afrane S, Jin C, Liu H, Fattah IMR, Show PL, Shouran M, Habil M, Kamel S. Progress and Recent Trends in the Application of Nanoparticles as Low Carbon Fuel Additives-A State of the Art Review. NANOMATERIALS (BASEL, SWITZERLAND) 2022; 12:1515. [PMID: 35564226 PMCID: PMC9101180 DOI: 10.3390/nano12091515] [Citation(s) in RCA: 5] [Impact Index Per Article: 1.7] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Subscribe] [Scholar Register] [Received: 03/22/2022] [Revised: 04/22/2022] [Accepted: 04/26/2022] [Indexed: 02/06/2023]
Abstract
The first part of the current review highlights the evolutionary nuances and research hotspots in the field of nanoparticles in low carbon fuels. Our findings reveal that contribution to the field is largely driven by researchers from Asia, mainly India. Of the three biofuels under review, biodiesel seems to be well studied and developed, whereas studies regarding vegetable oils and alcohols remain relatively scarce. The second part also reviews the application of nanoparticles in biodiesel/vegetable oil/alcohol-based fuels holistically, emphasizing fuel properties and engine characteristics. The current review reveals that the overall characteristics of the low carbon fuel-diesel blends improve under the influence of nanoparticles during combustion in diesel engines. The most important aspect of nanoparticles is that they act as an oxygen buffer that provides additional oxygen molecules in the combustion chamber, promoting complete combustion and lowering unburnt emissions. Moreover, the nanoparticles used for these purposes exhibit excellent catalytic behaviour as a result of their high surface area-to-volume ratio-this leads to a reduction in exhaust pollutants and ensures an efficient and complete combustion. Beyond energy-based indicators, the exergy, economic, environmental, and sustainability aspects of the blends in diesel engines are discussed. It is observed that the performance of the diesel engine fuelled with low carbon fuels according to the second law of efficiency improves under the influence of the nano-additives. Our final part shows that despite the benefits of nanoparticles, humans and animals are under serious threats from the highly toxic nature of nanoparticles.
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Affiliation(s)
- Jeffrey Dankwa Ampah
- School of Environmental Science and Engineering, Tianjin University, Tianjin 300072, China; (J.D.A.); (S.A.); (C.J.)
| | - Abdulfatah Abdu Yusuf
- Department of Mechanical and Automobile Engineering, Sharda University, Knowledge Park III, Greater Noida 201310, UP, India;
| | - Ephraim Bonah Agyekum
- Department of Nuclear and Renewable Energy, Ural Federal University Named after the First President of Russia Boris Yeltsin, 19 Mira Street, 620002 Ekaterinburg, Russia;
| | - Sandylove Afrane
- School of Environmental Science and Engineering, Tianjin University, Tianjin 300072, China; (J.D.A.); (S.A.); (C.J.)
| | - Chao Jin
- School of Environmental Science and Engineering, Tianjin University, Tianjin 300072, China; (J.D.A.); (S.A.); (C.J.)
| | - Haifeng Liu
- State Key Laboratory of Engines, Tianjin University, Tianjin 300072, China
| | - Islam Md Rizwanul Fattah
- Centre for Green Technology, Faculty of Engineering and IT, University of Technology Sydney, Ultimo, NSW 2007, Australia;
- Department of Mechanical Engineering, College of Engineering, Universiti Tenaga Nasional, Kajang 43000, Selangor Darul Ehsan, Malaysia
| | - Pau Loke Show
- Department of Chemical and Environmental Engineering, Faculty of Science and Engineering, University of Nottingham Malaysia, Jalan Broga, Semenyih 43500, Selangor Darul Ehsan, Malaysia;
| | - Mokhtar Shouran
- Wolfson Centre for Magnetics, School of Engineering, Cardiff University, Cardiff CF24 3AA, UK;
| | - Monier Habil
- Wolfson Centre for Magnetics, School of Engineering, Cardiff University, Cardiff CF24 3AA, UK;
| | - Salah Kamel
- Electrical Engineering Department, Faculty of Engineering, Aswan University, Aswan 81542, Egypt;
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Shahabi R, Dehghani M, Javad Moosavi SA, Shahabi B, Poordakan O, Sadeghi M, Aryan L, Ghasempoor A, Aghanasiri F, Mohseni M, Mehravi B. The effect of nanoparticles on pulmonary fibrosis: a systematic review and Meta-analysis of preclinical studies. ARCHIVES OF ENVIRONMENTAL & OCCUPATIONAL HEALTH 2022; 77:684-694. [PMID: 35244528 DOI: 10.1080/19338244.2021.2001637] [Citation(s) in RCA: 2] [Impact Index Per Article: 0.7] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Subscribe] [Scholar Register] [Indexed: 06/14/2023]
Abstract
Air pollution is a big ecumenical problem associated with public health around the world. The rapid development of nanotechnology worldwide resulted in a significant increase in human exposure with unknown particles, and ultimately leading to an increase in acute and chronic diseases. The effect of nanoparticles on pulmonary fibrosis has been reported in vivo and in vitro studies; however, the results are inconsistent. The present systematic review and meta-analysis of animal preclinical studies was conducted to assess the effect of nanoparticles on pulmonary fibrosis. A systematic search of online databases and gray literature as well as reference lists of retrieved studies was performed up to February 2019 to identify preclinical animal studies. Studies were assessed for methodological quality using the SYstematic Review Center for Laboratory animal Experimentation bias risk tool (SYRCLE's ROB tool). Pooled standardized mean difference (SMD) estimate with corresponding 95% CI was calculated using inverse-variance weights method while random effects meta-analysis was used, taking into account conceptual heterogeneity. To assess the robustness of pooled estimates as well as heterogeneity across studies, sensitivity analysis and Cochran Q statistic (with I2 statistic) was carried out using Stata 11.0. Of 6494 retrieved studies, 85 were reviewed in depth for eligibility. 16 studies met the criteria for inclusion in this systematic review. The meta-analysis was conducted on 10 studies which had reported the mean of TGF-β in 7 days after exposure by nanoparticles jointly (exposure compared to no exposure). Findings showed that exposure to nanoparticles significantly induced pulmonary fibrosis (SMD: 4.12, 95% CI: 2.57-5.67). A statistical heterogeneity was found [P < 0.001 (Q statistics), I2 = 83.0%] across studies. Nanoparticles were the most influencing in inducing pulmonary fibrosis in animal models. Sensitivity analysis demonstrated consistency of the results, indicating that the meta-analysis model was robust. Publication bias (using visual inspection and statistical tests) was unlikely in the association between nanoparticles and pulmonary fibrosis. We found that the nanoparticles significantly induce pulmonary fibrosis through increasing proinflammatory cytokine TGF-β and histopathological changes.
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Affiliation(s)
- Rana Shahabi
- Department of Physiology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran
| | - Mohsen Dehghani
- Department of Epidemiology, School of Public Health, Iran University of Medical Sciences, Tehran Iran
| | | | - Bahareh Shahabi
- Department of Psychology, Islamic Azad University Science and Research Branch of Tehran, Tehran, Iran
| | - Omid Poordakan
- Department of Medical Nanotechnology, Faculty of Medical Nanotechnologies, Iran University of Medical Sciences, Tehran, Iran
| | - Masoumeh Sadeghi
- Department of Epidemiology, Faculty of Health, Mashhad University of Medical Sciences, Mashhad, Iran
| | - Leila Aryan
- Department of Physiology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran
- R&D Division, Nanografi Co, METU, Technopolis, Ankara, Turkey
| | - Alireza Ghasempoor
- Department of Medical Nanotechnology, Faculty of Medical Nanotechnologies, Iran University of Medical Sciences, Tehran, Iran
| | - Fatemeh Aghanasiri
- Department of Medical Nanotechnology, Faculty of Medical Nanotechnologies, Iran University of Medical Sciences, Tehran, Iran
| | - Mojdeh Mohseni
- Radiation Biology Research Center, Iran University of Medical Sciences, Tehran, Iran
| | - Bita Mehravi
- Department of Medical Nanotechnology, Faculty of Medical Nanotechnologies, Iran University of Medical Sciences, Tehran, Iran
- Finetech in Medicine Research Center, Iran University of Medical Sciences, Tehran, Iran
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Sager TM, Umbright CM, Mustafa GM, Roberts JR, Orandle MS, Cumpston JL, McKinney WG, Boots T, Kashon ML, Joseph P. Pulmonary toxicity and gene expression changes in response to whole-body inhalation exposure to multi-walled carbon nanotubes in rats. Inhal Toxicol 2022; 34:200-218. [PMID: 35648795 PMCID: PMC9885491 DOI: 10.1080/08958378.2022.2081386] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 02/01/2023]
Abstract
Purpose: To investigate the molecular mechanisms underlying the pulmonary toxicity induced by exposure to one form of multi-walled carbon nanotubes (MWCNT-7).Materials and methods: Rats were exposed, by whole-body inhalation, to air or an aerosol containing MWCNT-7 particles at target cumulative doses (concentration x time) ranging from 22.5 to 180 (mg/m3)h over a three-day (6 hours/day) period and toxicity and global gene expression profiles were determined in the lungs.Results: MWCNT-7 particles, associated with alveolar macrophages (AMs), were detected in rat lungs following the exposure. Mild to moderate lung pathological changes consisting of increased cellularity, thickening of the alveolar wall, alveolitis, fibrosis, and granuloma formation were detected. Bronchoalveolar lavage (BAL) toxicity parameters such as lactate dehydrogenase activity, number of AMs and polymorphonuclear leukocytes (PMNs), intracellular oxidant generation by phagocytes, and levels of cytokines were significantly (p < 0.05) increased in response to exposure to MWCNT-7. Global gene expression profiling identified several significantly differentially expressed genes (fold change >1.5 and FDR p value <0.05) in all the MWCNT-7 exposed rats. Bioinformatic analysis of the gene expression data identified significant enrichment of several diseases/biological function categories (for example, cancer, leukocyte migration, inflammatory response, mitosis, and movement of phagocytes) and canonical pathways (for example, kinetochore metaphase signaling pathway, granulocyte and agranulocyte adhesion and diapedesis, acute phase response, and LXR/RXR activation). The alterations in the lung toxicity parameters and gene expression changes exhibited a dose-response to the MWCNT exposure.Conclusions: Taken together, the data provided insights into the molecular mechanisms underlying the pulmonary toxicity induced by inhalation exposure of rats to MWCNT-7.
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Affiliation(s)
- Tina M. Sager
- Health Effects Laboratory Division, National Institute for Occupational Safety and Health (NIOSH), Morgantown, WV, USA
| | - Christina M. Umbright
- Health Effects Laboratory Division, National Institute for Occupational Safety and Health (NIOSH), Morgantown, WV, USA
| | - Gul Mehnaz Mustafa
- Health Effects Laboratory Division, National Institute for Occupational Safety and Health (NIOSH), Morgantown, WV, USA
| | - Jenny R. Roberts
- Health Effects Laboratory Division, National Institute for Occupational Safety and Health (NIOSH), Morgantown, WV, USA
| | - Marlene S. Orandle
- Health Effects Laboratory Division, National Institute for Occupational Safety and Health (NIOSH), Morgantown, WV, USA
| | - Jared L. Cumpston
- Health Effects Laboratory Division, National Institute for Occupational Safety and Health (NIOSH), Morgantown, WV, USA
| | - Walter G. McKinney
- Health Effects Laboratory Division, National Institute for Occupational Safety and Health (NIOSH), Morgantown, WV, USA
| | - Theresa Boots
- Health Effects Laboratory Division, National Institute for Occupational Safety and Health (NIOSH), Morgantown, WV, USA
| | - Michael L. Kashon
- Health Effects Laboratory Division, National Institute for Occupational Safety and Health (NIOSH), Morgantown, WV, USA
| | - Pius Joseph
- Health Effects Laboratory Division, National Institute for Occupational Safety and Health (NIOSH), Morgantown, WV, USA
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Fujita K, Obara S, Maru J. Pulmonary toxicity, cytotoxicity, and genotoxicity of submicron-diameter carbon fibers with different diameters and lengths. Toxicology 2021; 466:153063. [PMID: 34890706 DOI: 10.1016/j.tox.2021.153063] [Citation(s) in RCA: 5] [Impact Index Per Article: 1.3] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 09/13/2021] [Revised: 11/30/2021] [Accepted: 12/06/2021] [Indexed: 12/30/2022]
Abstract
Submicron-diameter carbon fibers (SCFs) are a type of fine-diameter fibrous carbon material that can be used in various applications. To accelerate their practical application, a hazard assessment of SCFs must be undertaken. This study demonstrated the pulmonary toxicity, cytotoxicity, and genotoxicity of three types of SCFs with different diameters and lengths. The average diameter and length of SCFs were 259.2 nm and 11.7 μm in SCF1 suspensions, 248.5 nm and 6.7 μm in SCF2 suspensions, and 183.0 nm and 13.7 μm in SCF3 suspensions, respectively. The results of pulmonary inflammation and recovery following intratracheal instillation with SCFs at doses of 0.25, 0.5, or 1.0 mg/kg showed that the pulmonary toxicity of SCFs was SCF3 > SCF1 > SCF2. These results suggest that SCF diameter and length are most likely important contributing factors associated with lung SCF clearance, pulmonary inflammation, and recovery. Furthermore, SCFs are less pulmonary toxic than bent multi-walled carbon nanotubes. Cell viability, pro-inflammatory cytokine and intracellular reactive oxygen species productions, morphological changes, gene expression profiling in NR8383 rat alveolar macrophage cells showed that the cytotoxic potency of SCFs is: SCF3 > SCF1 > SCF2. These results showed that SCFs with small diameters had high cytotoxicity, and SCFs with short lengths had low cytotoxicity. We conclude that pulmonary toxicity and cytotoxicity are associated with the diameter and length distributions of SCFs. In addition, a standard battery for genotoxicity testing, namely the Ames test, an in vitro chromosomal aberration test, and a mammalian erythrocyte micronucleus test, demonstrated that the three types of SCFs did not induce genotoxicity. Our findings provide new evidence for evaluating the potential toxicity of not only SCFs used in this study but also various SCFs which differ depending on the manufacturing processes or physicochemical properties.
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Affiliation(s)
- Katsuhide Fujita
- Research Institute of Science for Safety and Sustainability (RISS), National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Ibaraki, 305-8569, Japan.
| | - Sawae Obara
- Research Institute of Science for Safety and Sustainability (RISS), National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Ibaraki, 305-8569, Japan
| | - Junko Maru
- Research Institute of Science for Safety and Sustainability (RISS), National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Ibaraki, 305-8569, Japan
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Mohapatra A, Sathiyamoorthy P, Park IK. Metallic Nanoparticle-Mediated Immune Cell Regulation and Advanced Cancer Immunotherapy. Pharmaceutics 2021; 13:1867. [PMID: 34834282 PMCID: PMC8622235 DOI: 10.3390/pharmaceutics13111867] [Citation(s) in RCA: 20] [Impact Index Per Article: 5.0] [Reference Citation Analysis] [Abstract] [Key Words] [Grants] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 10/09/2021] [Revised: 10/30/2021] [Accepted: 11/01/2021] [Indexed: 12/13/2022] Open
Abstract
Cancer immunotherapy strategies leveraging the body's own immune system against cancer cells have gained significant attention due to their remarkable therapeutic efficacy. Several immune therapies have been approved for clinical use while expanding the modalities of cancer therapy. However, they are still not effective in a broad range of cancer patients because of the typical immunosuppressive microenvironment and limited antitumor immunity achieved with the current treatment. Novel approaches, such as nanoparticle-mediated cancer immunotherapies, are being developed to overcome these challenges. Various types of nanoparticles, including liposomal, polymeric, and metallic nanoparticles, are reported for the development of effective cancer therapeutics. Metallic nanoparticles (MNPs) are one of the promising candidates for anticancer therapy due to their unique theranostic properties and are thus explored as both imaging and therapeutic agents. In addition, MNPs offer a dense surface functionalization to target tumor tissue and deliver genetic, therapeutic, and immunomodulatory agents. Furthermore, MNPs interact with the tumor microenvironment (TME) and regulate the levels of tumor hypoxia, glutathione (GSH), and reactive oxygen species (ROS) for remodulation of TME for successful therapy. In this review, we discuss the role of nanoparticles in tumor microenvironment modulation and anticancer therapy. In particular, we evaluated the response of MNP-mediated immune cells, such as dendritic cells, macrophages, T cells and NK cells, against tumor cells and analyzed the role of MNP-based cancer therapies in regulating the immunosuppressive environment.
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Affiliation(s)
- Adityanarayan Mohapatra
- Department of Biomedical Sciences, Chonnam National University Medical School, Hwasun 58128, Korea; (A.M.); (P.S.)
- Biomedical Science Graduate Program (BMSGP), Chonnam National University, Hwasun 58128, Korea
| | - Padmanaban Sathiyamoorthy
- Department of Biomedical Sciences, Chonnam National University Medical School, Hwasun 58128, Korea; (A.M.); (P.S.)
- Biomedical Science Graduate Program (BMSGP), Chonnam National University, Hwasun 58128, Korea
| | - In-Kyu Park
- Department of Biomedical Sciences, Chonnam National University Medical School, Hwasun 58128, Korea; (A.M.); (P.S.)
- Biomedical Science Graduate Program (BMSGP), Chonnam National University, Hwasun 58128, Korea
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Sorout N, Chandra A. Interactions of the Aβ(1-42) Peptide with Boron Nitride Nanoparticles of Varying Curvature in an Aqueous Medium: Different Pathways to Inhibit β-Sheet Formation. J Phys Chem B 2021; 125:11159-11178. [PMID: 34605235 DOI: 10.1021/acs.jpcb.1c05805] [Citation(s) in RCA: 4] [Impact Index Per Article: 1.0] [Reference Citation Analysis] [Abstract] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 01/16/2023]
Abstract
The aggregation of amyloid β (Aβ) peptide triggered by its conformational changes leads to the commonly known neurodegenerative disease of Alzheimer's. It is believed that the formation of β sheets of the peptide plays a key role in its aggregation and subsequent fibrillization. In the current study, we have investigated the interactions of the Aβ(1-42) peptide with boron nitride nanoparticles and the effects of the latter on conformational transitions of the peptide through a series of molecular dynamics simulations. In particular, the effects of curvature of the nanoparticle surface are studied by considering boron nitride nanotubes (BNNTs) of varying diameter and also a planar boron nitride nanosheet (BNNS). Altogether, the current study involves the generation and analysis of 9.5 μs of dynamical trajectories of peptide-BNNT/BNNS pairs in an aqueous medium. It is found that BN nanoparticles of different curvatures that are studied in the present work inhibit the conformational transition of the peptide to its β-sheet form. However, such an inhibition effect follows different pathways for BN nanoparticles of different curvatures. For the BNNT with the highest surface curvature, i.e., (3,3) BNNT, the nanoparticle is found to inhibit β-sheet formation by stabilizing the helical structure of the peptide, whereas for planar BNNS, the β-sheet formation is prevented by making more favorable pathways available for transitions of the peptide to conformations of random coils and turns. The BNNTs with intermediate curvatures are found to exhibit diverse pathways of their interactions with the peptide, but in all cases, essentially no formation of the β sheet is found whereas substantial β-sheet formation is observed for Aβ(1-42) in water in the absence of any nanoparticle. The current study shows that BN nanoparticles have the potential to act as effective tools to prevent amyloid formation from Aβ peptides.
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Affiliation(s)
- Nidhi Sorout
- Department of Chemistry, Indian Institute of Technology Kanpur, Uttar Pradesh, India 208016
| | - Amalendu Chandra
- Department of Chemistry, Indian Institute of Technology Kanpur, Uttar Pradesh, India 208016
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Ahmed HM, Roy A, Wahab M, Ahmed M, Othman-Qadir G, Elesawy BH, Khandaker MU, Islam MN, Emran TB. Applications of Nanomaterials in Agrifood and Pharmaceutical Industry. JOURNAL OF NANOMATERIALS 2021; 2021:1-10. [DOI: 10.1155/2021/1472096] [Citation(s) in RCA: 19] [Impact Index Per Article: 4.8] [Reference Citation Analysis] [Abstract] [Track Full Text] [Subscribe] [Scholar Register] [Indexed: 09/01/2023]
Abstract
Nanotechnology recently emerged among the most exciting science-related innovations. Nanotechnology-produced metal nanoparticles got a lot of attention. This is emerging as a rapidly developing field due to its effective applications that targeted the manufacturing of new materials at the nanoscale level. There is considerable interest in the application of nanomaterials in many areas of industry including agrifood and biomedical products. In the agrifood area, nanomaterials have benefits in diverse areas which include fertilizers, herbicides, pesticides, sensors, and quality stimulants, among other food processing, food packaging, and nutraceuticals to improve nutritional value. These applications in agriculture result in enhanced quality and crop yield, reduction in pollution caused by various chemicals, etc. In the pharmaceutical area, nanomaterials are claimed to ameliorate drug safety and efficacy, as well as bioavailability. They are utilized for targeting various drugs to a specific location in the body. However, there are also concerns that some nanoparticles may have adverse effects on human health. These include titanium dioxide, copper oxides, and other nanomaterials which lead to liver damage, skin damage, lung damage, and various other human health-related problems. This review is aimed at presenting a briefing on the state of the art in the application of nanotechnology in food and human nutrition and drug administration, consumer attitudes, and their challenges and opportunities with future perspectives.
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Affiliation(s)
- Hiwa M. Ahmed
- Sulaimani Polytechnic University, Slemani, 46001 Kurdistan Region, Iraq
| | - Arpita Roy
- Department of Biotechnology, School of Engineering & Technology, Sharda University, Greater Noida, India
| | - Muhammad Wahab
- Food Science and Quality Control Department, College of Agricultural Engineering Science, University of Sulaimani, Slemani, Kurdistan Region, Iraq
| | - Mohammed Ahmed
- Department of Horticulture, University of Raparin, Ranya, Kurdistan Region, Iraq
| | - Gashaw Othman-Qadir
- Newcastle Center for Natural Therapy, Slemani, Ranya, 46012 Kurdistan Region, Iraq
| | - Basem H. Elesawy
- Department of Pathology, College of Medicine, Taif University, P.O. Box 11099, Taif 21944, Saudi Arabia
| | - Mayeen Uddin Khandaker
- Centre for Applied Physics and Radiation Technologies, School of Engineering and Technology, Sunway University, 47500 Selangor, Malaysia
| | - Mohammad Nazmul Islam
- Department of Pharmacy, International Islamic University Chittagong, Chittagong 4318, Bangladesh
| | - Talha Bin Emran
- Department of Pharmacy, BGC Trust University Bangladesh, Chittagong 4381, Bangladesh
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A state-of-the-art review on the application of various pharmaceutical nanoparticles as a promising technology in cancer treatment. ARAB J CHEM 2021. [DOI: 10.1016/j.arabjc.2021.103352] [Citation(s) in RCA: 13] [Impact Index Per Article: 3.3] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 12/12/2022] Open
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Lu RXZ, Radisic M. Organ-on-a-chip platforms for evaluation of environmental nanoparticle toxicity. Bioact Mater 2021; 6:2801-2819. [PMID: 33665510 PMCID: PMC7900603 DOI: 10.1016/j.bioactmat.2021.01.021] [Citation(s) in RCA: 36] [Impact Index Per Article: 9.0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 10/25/2020] [Revised: 01/20/2021] [Accepted: 01/20/2021] [Indexed: 02/07/2023] Open
Abstract
Despite showing a great promise in the field of nanomedicine, nanoparticles have gained a significant attention from regulatory agencies regarding their possible adverse health effects upon environmental exposure. Whether those nanoparticles are generated through intentional or unintentional means, the constant exposure to nanomaterials can inevitably lead to unintended consequences based on epidemiological data, yet the current understanding of nanotoxicity is insufficient relative to the rate of their emission in the environment and the lack of predictive platforms that mimic the human physiology. This calls for a development of more physiologically relevant models, which permit the comprehensive and systematic examination of toxic properties of nanoparticles. With the advancement in microfabrication techniques, scientists have shifted their focus on the development of an engineered system that acts as an intermediate between a well-plate system and animal models, known as organ-on-a-chips. The ability of organ-on-a-chip models to recapitulate in vivo like microenvironment and responses offers a new avenue for nanotoxicological research. In this review, we aim to provide overview of assessing potential risks of nanoparticle exposure using organ-on-a-chip systems and their potential to delineate biological mechanisms of epidemiological findings.
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Affiliation(s)
- Rick Xing Ze Lu
- Institute of Biomedical Engineering, University of Toronto, Toronto, ON, Canada
| | - Milica Radisic
- Institute of Biomedical Engineering, University of Toronto, Toronto, ON, Canada
- Department of Chemical Engineering and Applied Chemistry, University of Toronto, Toronto, ON, Canada
- Toronto General Research Institute, University Health Network, Toronto, ON, Canada
- The Heart and Stroke/Richard Lewar Centre of Excellence, Toronto, ON, Canada
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40
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Marczynski M, Lieleg O. Forgotten but not gone: Particulate matter as contaminations of mucosal systems. BIOPHYSICS REVIEWS 2021; 2:031302. [PMID: 38505633 PMCID: PMC10903497 DOI: 10.1063/5.0054075] [Citation(s) in RCA: 1] [Impact Index Per Article: 0.3] [Reference Citation Analysis] [Abstract] [Track Full Text] [Subscribe] [Scholar Register] [Received: 04/14/2021] [Accepted: 07/14/2021] [Indexed: 03/21/2024]
Abstract
A decade ago, environmental issues, such as air pollution and the contamination of the oceans with microplastic, were prominently communicated in the media. However, these days, political topics, as well as the ongoing COVID-19 pandemic, have clearly taken over. In spite of this shift in focus regarding media representation, researchers have made progress in evaluating the possible health risks associated with particulate contaminations present in water and air. In this review article, we summarize recent efforts that establish a clear link between the increasing occurrence of certain pathological conditions and the exposure of humans (or animals) to airborne or waterborne particulate matter. First, we give an overview of the physiological functions mucus has to fulfill in humans and animals, and we discuss different sources of particulate matter. We then highlight parameters that govern particle toxicity and summarize our current knowledge of how an exposure to particulate matter can be related to dysfunctions of mucosal systems. Last, we outline how biophysical tools and methods can help researchers to obtain a better understanding of how particulate matter may affect human health. As we discuss here, recent research has made it quite clear that the structure and functions of those mucosal systems are sensitive toward particulate contaminations. Yet, our mechanistic understanding of how (and which) nano- and microparticles can compromise human health via interacting with mucosal barriers is far from complete.
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Arezki Y, Cornacchia J, Rapp M, Lebeau L, Pons F, Ronzani C. A Co-Culture Model of the Human Respiratory Tract to Discriminate the Toxicological Profile of Cationic Nanoparticles According to Their Surface Charge Density. TOXICS 2021; 9:210. [PMID: 34564361 PMCID: PMC8470030 DOI: 10.3390/toxics9090210] [Citation(s) in RCA: 1] [Impact Index Per Article: 0.3] [Reference Citation Analysis] [Abstract] [Key Words] [Grants] [Track Full Text] [Download PDF] [Figures] [Subscribe] [Scholar Register] [Received: 06/23/2021] [Revised: 08/27/2021] [Accepted: 08/28/2021] [Indexed: 12/16/2022]
Abstract
This study aimed at discriminating with sensitivity the toxicological effects of carbon dots (CDs) with various zeta potential (ζ) and charge density (Qek) in different cellular models of the human respiratory tract. One anionic and three cationic CDs were synthetized as follows: CD-COOH (ζ = -43.3 mV); CD-PEI600 (Qek = 4.70 µmol/mg; ζ = +31.8 mV); CD-PEHA (Qek = 3.30 µmol/mg; ζ = +29.2 mV) and CD-DMEDA (Qek = 0.01 µmol/mg; ζ = +11.1 mV). Epithelial cells (A549) and macrophages (THP-1) were seeded alone or as co-cultures with different A549:THP-1 ratios. The obtained models were characterized, and multiple biological responses evoked by CDs were assessed in the mono-cultures and the best co-culture model. With 14% macrophages, the 2:1 ratio co-culture best mimicked the in vivo conditions and responded to lipopolysaccharides. The anionic CD did not induce any effect in the mono-cultures nor in the co-culture. Among the cationic CDs, the one with the highest charge density (CD-PEI600) induced the most pronounced responses whatever the culture model. The cationic CDs of low charge density (CD-PEHA and CD-DMEDA) evoked similar responses in the mono-cultures, whereas in the co-culture, the three cationic CDs ranked according to their charge density (CD-PEI600 > CD-PEHA > CD-DMEDA), when taking into account their inflammatory effect. Thus, the co-culture system developed in this study appears to be a sensitive model for finely discriminating the toxicological profile of cationic nanoparticles differing by the density of their surface charges.
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Affiliation(s)
| | | | | | | | | | - Carole Ronzani
- Laboratoire de Conception et Application de Molécules Bioactives, Faculté de Pharmacie, UMR 7199, CNRS-Université de Strasbourg, 67400 Illkirch, France; (Y.A.); (J.C.); (M.R.); (L.L.); (F.P.)
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Toxicity of Nanoparticles in Biomedical Application: Nanotoxicology. J Toxicol 2021; 2021:9954443. [PMID: 34422042 PMCID: PMC8376461 DOI: 10.1155/2021/9954443] [Citation(s) in RCA: 127] [Impact Index Per Article: 31.8] [Reference Citation Analysis] [Abstract] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 03/07/2021] [Accepted: 07/15/2021] [Indexed: 11/17/2022] Open
Abstract
Nanoparticles are of great importance in development and research because of their application in industries and biomedicine. The development of nanoparticles requires proper knowledge of their fabrication, interaction, release, distribution, target, compatibility, and functions. This review presents a comprehensive update on nanoparticles' toxic effects, the factors underlying their toxicity, and the mechanisms by which toxicity is induced. Recent studies have found that nanoparticles may cause serious health effects when exposed to the body through ingestion, inhalation, and skin contact without caution. The extent to which toxicity is induced depends on some properties, including the nature and size of the nanoparticle, the surface area, shape, aspect ratio, surface coating, crystallinity, dissolution, and agglomeration. In all, the general mechanisms by which it causes toxicity lie on its capability to initiate the formation of reactive species, cytotoxicity, genotoxicity, and neurotoxicity, among others.
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Mostovenko E, Saunders S, Muldoon PP, Bishop L, Campen MJ, Erdely A, Ottens AK. Carbon Nanotube Exposure Triggers a Cerebral Peptidomic Response: Barrier Compromise, Neuroinflammation, and a Hyperexcited State. Toxicol Sci 2021; 182:107-119. [PMID: 33892499 DOI: 10.1093/toxsci/kfab042] [Citation(s) in RCA: 8] [Impact Index Per Article: 2.0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 01/15/2023] Open
Abstract
The unique physicochemical properties of carbon nanomaterials and their ever-growing utilization generate a serious concern for occupational risk. Pulmonary exposure to these nanoparticles induces local and systemic inflammation, cardiovascular dysfunction, and even cognitive deficits. Although multiple routes of extrapulmonary toxicity have been proposed, the mechanism for and manner of neurologic effects remain minimally understood. Here, we examine the cerebral spinal fluid (CSF)-derived peptidomic fraction as a reflection of neuropathological alterations induced by pulmonary carbon nanomaterial exposure. Male C57BL/6 mice were exposed to 10 or 40 µg of multiwalled carbon nanotubes (MWCNT) by oropharyngeal aspiration. Serum and CSFs were collected 4 h post exposure. An enriched peptide fraction of both biofluids was analyzed using ion mobility-enabled data-independent mass spectrometry for label-free quantification. MWCNT exposure induced a prominent peptidomic response in the blood and CSF; however, correlation between fluids was limited. Instead, we determined that a MWCNT-induced peptidomic shift occurred specific to the CSF with 292 significant responses found that were not in serum. Identified MWCNT-responsive peptides depicted a mechanism involving aberrant fibrinolysis (fibrinopeptide A), blood-brain barrier permeation (homeobox protein A4), neuroinflammation (transmembrane protein 131L) with reactivity by astrocytes and microglia, and a pro-degradative (signal transducing adapter molecule, phosphoglycerate kinase), antiplastic (AF4/FMR2 family member 1, vacuolar protein sorting-associated protein 18) state with the excitation-inhibition balance shifted to a hyperexcited (microtubule-associated protein 1B) phenotype. Overall, the significant pathologic changes observed were consistent with early neurodegenerative disease and were diagnostically reflected in the CSF peptidome.
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Affiliation(s)
- Ekaterina Mostovenko
- Department of Anatomy and Neurobiology, Virginia Commonwealth University, Richmond, Virginia 23298, USA
| | - Samantha Saunders
- Department of Anatomy and Neurobiology, Virginia Commonwealth University, Richmond, Virginia 23298, USA
| | - Pretal P Muldoon
- Department of Anatomy and Neurobiology, Virginia Commonwealth University, Richmond, Virginia 23298, USA
| | - Lindsey Bishop
- Pathology and Physiology Research Branch, National Institute for Occupational Safety and Health, Morgantown, West Virginia 26505, USA
| | - Matthew J Campen
- Department of Pharmaceutical Sciences, University of New Mexico, Albuquerque, New Mexico 87131, USA
| | - Aaron Erdely
- Pathology and Physiology Research Branch, National Institute for Occupational Safety and Health, Morgantown, West Virginia 26505, USA
| | - Andrew K Ottens
- Department of Anatomy and Neurobiology, Virginia Commonwealth University, Richmond, Virginia 23298, USA
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Zhang C, Wu L, de Perrot M, Zhao X. Carbon Nanotubes: A Summary of Beneficial and Dangerous Aspects of an Increasingly Popular Group of Nanomaterials. Front Oncol 2021; 11:693814. [PMID: 34386422 PMCID: PMC8353320 DOI: 10.3389/fonc.2021.693814] [Citation(s) in RCA: 16] [Impact Index Per Article: 4.0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 04/12/2021] [Accepted: 07/08/2021] [Indexed: 11/13/2022] Open
Abstract
Carbon nanotubes (CNTs) are nanomaterials with broad applications that are produced on a large scale. Animal experiments have shown that exposure to CNTs, especially one type of multi-walled carbon nanotube, MWCNT-7, can lead to malignant transformation. CNTs have characteristics similar to asbestos (size, shape, and biopersistence) and use the same molecular mechanisms and signaling pathways as those involved in asbestos tumorigenesis. Here, a comprehensive review of the characteristics of carbon nanotubes is provided, as well as insights that may assist in the design and production of safer nanomaterials to limit the hazards of currently used CNTs.
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Affiliation(s)
- Chengke Zhang
- Department of Thoracic Surgery, The Second Hospital, Cheeloo College of Medicine, Shandong University, Jinan, China
- Key Laboratory of Thoracic Cancer, Cheeloo College of Medicine, Shandong University, Jinan, China
| | - Licun Wu
- Key Laboratory of Thoracic Cancer, Cheeloo College of Medicine, Shandong University, Jinan, China
- Latner Thoracic Surgery Research Laboratories and Division of Thoracic Surgery, Toronto General Hospital, University Health Network, University of Toronto, Toronto, ON, Canada
| | - Marc de Perrot
- Latner Thoracic Surgery Research Laboratories and Division of Thoracic Surgery, Toronto General Hospital, University Health Network, University of Toronto, Toronto, ON, Canada
- Department Immunology, University of Toronto, Toronto, ON, Canada
| | - Xiaogang Zhao
- Department of Thoracic Surgery, The Second Hospital, Cheeloo College of Medicine, Shandong University, Jinan, China
- Key Laboratory of Thoracic Cancer, Cheeloo College of Medicine, Shandong University, Jinan, China
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Nabi SU, Ali SI, Rather MA, Sheikh WM, Altaf M, Singh H, Mumtaz PT, Mishra NC, Nazir SU, Bashir SM. Organoids: A new approach in toxicity testing of nanotherapeutics. J Appl Toxicol 2021; 42:52-72. [PMID: 34060108 DOI: 10.1002/jat.4206] [Citation(s) in RCA: 19] [Impact Index Per Article: 4.8] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 03/18/2021] [Revised: 05/04/2021] [Accepted: 05/20/2021] [Indexed: 12/18/2022]
Abstract
Nanotechnology has revolutionized diverse fields, which include agriculture, the consumer market, medicine, and other fields. Widespread use of nanotechnology-based products has led to increased prevalence of these novel formulations in the environment, which has raised concerns regarding their deleterious effects. The application of nanotechnology-based formulations into clinical use is hampered by the lack of the availability of effective in vitro systems, which could accurately assess their in vivo toxic effects. A plethora of studies has shown the hazardous effects of nanoparticle-based formulations in two-dimensional in vitro cell cultures and animal models. These have some associated disadvantages when used for the evaluation of nano-toxicity. Organoid technology fills the space between existing two-dimensional cell line culture and in vivo models. The uniqueness of organoids over other systems for evaluating toxicity caused by nano-drug formulation includes them being a co-culture of diverse cell types, dynamic flow within them that simulates the actual flow of nanoparticles within biological systems, extensive cell-cell, cell-matrix interactions, and a tissue-like morphology. Thus, it mimics the actual tissue microenvironment and, subsequently, provides an opportunity to study drug metabolism and toxico-dynamics of nanotechnology-based novel formulations. The use of organoids in the evaluation of nano-drug toxicity is in its infancy. A limited number of studies conducted so far have shown good predictive value and efficiently significant data correlation with the clinical trials. In this review, we attempt to introduce organoids of the liver, lungs, brain, kidney intestine, and potential applications to evaluate toxicity caused by nanoparticles.
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Affiliation(s)
- Showkat Ul Nabi
- Large Animal Diagnostic Laboratory, Department of Clinical Veterinary Medicine, Ethics & Jurisprudence, Division of Veterinary Biochemistry, Faculty of Veterinary Sciences and Animal Husbandry, Sher-e-Kashmir University of Agricultural Sciences and Technology, Srinagar, Jammu and Kashmir, India
| | - Sofi Imtiyaz Ali
- Biochemistry & Molecular Biology Lab, Division of Veterinary Biochemistry, Faculty of Veterinary Sciences and Animal Husbandry, Sher-e-Kashmir University of Agricultural Sciences and Technology, Srinagar, Jammu and Kashmir, India
| | - Muzafar Ahmad Rather
- Biochemistry & Molecular Biology Lab, Division of Veterinary Biochemistry, Faculty of Veterinary Sciences and Animal Husbandry, Sher-e-Kashmir University of Agricultural Sciences and Technology, Srinagar, Jammu and Kashmir, India
| | - Wajid Mohammad Sheikh
- Biochemistry & Molecular Biology Lab, Division of Veterinary Biochemistry, Faculty of Veterinary Sciences and Animal Husbandry, Sher-e-Kashmir University of Agricultural Sciences and Technology, Srinagar, Jammu and Kashmir, India
| | - Mehvish Altaf
- Department of Food Technology, Islamic University of Science & Technology, Awantipora, Pulwama, Jammu and Kashmir, India
| | - Hemant Singh
- Department of Polymer and Process Engineering, Indian Institute of Technology Roorkee, Roorkee, India
| | - Peerzada Tajamul Mumtaz
- Biochemistry & Molecular Biology Lab, Division of Veterinary Biochemistry, Faculty of Veterinary Sciences and Animal Husbandry, Sher-e-Kashmir University of Agricultural Sciences and Technology, Srinagar, Jammu and Kashmir, India
| | - Narayan Chandra Mishra
- Department of Polymer and Process Engineering, Indian Institute of Technology Roorkee, Roorkee, India
| | - Sheikh Uzma Nazir
- Biochemistry & Molecular Biology Lab, Division of Veterinary Biochemistry, Faculty of Veterinary Sciences and Animal Husbandry, Sher-e-Kashmir University of Agricultural Sciences and Technology, Srinagar, Jammu and Kashmir, India
| | - Showkeen Muzamil Bashir
- Biochemistry & Molecular Biology Lab, Division of Veterinary Biochemistry, Faculty of Veterinary Sciences and Animal Husbandry, Sher-e-Kashmir University of Agricultural Sciences and Technology, Srinagar, Jammu and Kashmir, India
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Zhang X, Luo M, Zhang J, Yao Z, Zhu J, Yang S, Zhu Q, Shen T. Carbon nanotubes promote alveolar macrophages toward M2 polarization mediated epithelial-mesenchymal transition and fibroblast-to-myofibroblast transdifferentiation. Nanotoxicology 2021; 15:588-604. [PMID: 33840345 DOI: 10.1080/17435390.2021.1905098] [Citation(s) in RCA: 12] [Impact Index Per Article: 3.0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 12/31/2022]
Abstract
With substantial progress of nanotechnology, carbon nanotubes (CNTs) are widely used in a variety of industrial and commercial applications. There is rising concern about potential adverse health effects, such as pulmonary fibrosis, related to inhalation of CNTs. The detailed cellular and molecular mechanisms of pulmonary fibrosis induced by CNTs are still not clear. Epithelial-mesenchymal transition (EMT) and fibroblast-to-myofibroblast transdifferentiation (FMT) are considered as critical events in pathogenesis of pulmonary fibrosis. Alveolar macrophages (AMs) polarization plays a key role of regulating EMT and FMT in pulmonary fibrosis. In this study, we applied CNTs to stimulate primary mouse AMs under M1 or M2 polarization conditions, then analyzed the proportion of F4/80+CD11c+ or F4/80+CD206+ AMs, mRNA expression and activities of iNOS or Arg-1, as well as mRNA expression and content of TNF-α and IL-6 or TGF-β and IL-10 to evaluate dynamic phenotypic and functional changes of AMs. Single-walled CNT (SWCNT), short-type multi-walled CNT (MWCNT), and long-type MWCNT exposure at dose of 50 µg/ml promote AMs polarization toward M1 phenotype at early stage, while promote AMs polarization toward M2 phenotype at late stage. The roles of AMs polarization during development of EMT and FMT were further investigated by conditioned medium (CM) experiments. CNTs-activated M2 AMs promote progression of EMT and FMT via secreting TGF-β. Furthermore, up-regulating IRF4 may be involved in CNTs-induced M2 AMs polarization. In conclusion, this study demonstrates a new insight that CNTs exposure promotes AMs polarization toward M2 phenotype which facilitate EMT and FMT through secreting TGF-β.
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Affiliation(s)
- Xiang Zhang
- Department of Occupational Health and Environment Health, School of Public Health, Anhui Medical University, Hefei, PR China
| | - Min Luo
- Department of Occupational Health and Environment Health, School of Public Health, Anhui Medical University, Hefei, PR China
| | - Jiaxiang Zhang
- Department of Occupational Health and Environment Health, School of Public Health, Anhui Medical University, Hefei, PR China
| | - Zhuomeng Yao
- Department of Occupational Health and Environment Health, School of Public Health, Anhui Medical University, Hefei, PR China
| | - Jiaojiao Zhu
- Department of Occupational Health and Environment Health, School of Public Health, Anhui Medical University, Hefei, PR China
| | - Shuxin Yang
- Department of Medical Aspects of Specific Environments, School of Basic Medicine, Anhui Medical University, Hefei, PR China
| | - Qixing Zhu
- Department of Occupational Health and Environment Health, School of Public Health, Anhui Medical University, Hefei, PR China
| | - Tong Shen
- Department of Occupational Health and Environment Health, School of Public Health, Anhui Medical University, Hefei, PR China
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Murphy F, Dekkers S, Braakhuis H, Ma-Hock L, Johnston H, Janer G, di Cristo L, Sabella S, Jacobsen NR, Oomen AG, Haase A, Fernandes T, Stone V. An integrated approach to testing and assessment of high aspect ratio nanomaterials and its application for grouping based on a common mesothelioma hazard. NANOIMPACT 2021; 22:100314. [PMID: 35559971 DOI: 10.1016/j.impact.2021.100314] [Citation(s) in RCA: 28] [Impact Index Per Article: 7.0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Subscribe] [Scholar Register] [Received: 11/02/2020] [Revised: 02/25/2021] [Accepted: 03/25/2021] [Indexed: 06/15/2023]
Abstract
Here we describe the development of an Integrated Approach to Testing and Assessment (IATA) to support the grouping of different types (nanoforms; NFs) of High Aspect Ratio Nanomaterials (HARNs), based on their potential to cause mesothelioma. Hazards posed by the inhalation of HARNs are of particular concern as they exhibit physical characteristics similar to pathogenic asbestos fibres. The approach for grouping HARNs presented here is part of a framework to provide guidance and tools to group similar NFs and aims to reduce the need to assess toxicity on a case-by-case basis. The approach to grouping is hypothesis-driven, in which the hypothesis is based on scientific evidence linking critical physicochemical descriptors for NFs to defined fate/toxicokinetic and hazard outcomes. The HARN IATA prompts users to address relevant questions (at decision nodes; DNs) regarding the morphology, biopersistence and inflammatory potential of the HARNs under investigation to provide the necessary evidence to accept or reject the grouping hypothesis. Each DN in the IATA is addressed in a tiered manner, using data from simple in vitro or in silico methods in the lowest tier or from in vivo approaches in the highest tier. For these proposed methods we provide justification for the critical descriptors and thresholds that allow grouping decisions to be made. Application of the IATA allows the user to selectively identify HARNs which may pose a mesothelioma hazard, as demonstrated through a literature-based case study. By promoting the use of alternative, non-rodent approaches such as in silico modelling, in vitro and cell-free tests in the initial tiers, the IATA testing strategy streamlines information gathering at all stages of innovation through to regulatory risk assessment while reducing the ethical, time and economic burden of testing.
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Affiliation(s)
- Fiona Murphy
- NanoSafety Group, Heriot-Watt University, Edinburgh, UK.
| | - Susan Dekkers
- National Institute for Public Health and the Environment (RIVM), Bilthoven, the Netherlands
| | - Hedwig Braakhuis
- National Institute for Public Health and the Environment (RIVM), Bilthoven, the Netherlands
| | - Lan Ma-Hock
- BASF SE, Dept. Material Physics and Dept of Experimental Toxicology & Ecology, Ludwigshafen, Germany
| | | | - Gemma Janer
- LEITAT Technological Center, Barcelona, Spain
| | | | | | | | - Agnes G Oomen
- National Institute for Public Health and the Environment (RIVM), Bilthoven, the Netherlands
| | - Andrea Haase
- German Federal Institute for Risk Assessment (BfR), Department of Chemical and Product Safety, Berlin, Germany
| | | | - Vicki Stone
- NanoSafety Group, Heriot-Watt University, Edinburgh, UK
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48
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Ahangarpour A, Alboghobeish S, Oroojan AA, Dehghani MA. Caffeic acid protects mice pancreatic islets from oxidative stress induced by multi-walled carbon nanotubes (MWCNTs). VETERINARY RESEARCH FORUM : AN INTERNATIONAL QUARTERLY JOURNAL 2021; 12:77-85. [PMID: 33953877 PMCID: PMC8094137 DOI: 10.30466/vrf.2019.94666.2279] [Citation(s) in RCA: 2] [Impact Index Per Article: 0.5] [Reference Citation Analysis] [Abstract] [Key Words] [Download PDF] [Figures] [Subscribe] [Scholar Register] [Received: 09/29/2018] [Accepted: 02/04/2019] [Indexed: 12/27/2022]
Abstract
Increasing applications of carbon nanotubes (CNTs) indicate the necessity to examine their toxicity. According to previous studies, CNTs caused oxidative stress that impaired β-cell functions and reduced insulin secretion. Our previous study indicated that single-walled carbon nanotubes (SWCNTs) could induce oxidative stress in pancreatic islets. However, there is no study on the effects of multi-walled carbon nanotubes (MWCNTs) on islets and β-cells. Therefore, the present study aims to evaluate effects of MWCNTs on the oxidative stress of islets and the protective effects of caffeic acid (CA) as an antioxidant. The effects of MWCNTs and CA on islets were investigated using MTT assay, reactive oxygen species (ROS), malondialdehyde (MDA), activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px), the content of glutathione (GSH) and mitochondrial membrane potential (MMP) and insulin secretion measurements. The lower viability of islet cells was dose-dependent due to the exposure to MWCNTs according to the MTT assay. Further studies revealed that MWCNTs decreased insulin secretion and MMP, induced ROS creation, increased the MDA level, and decreased activities of SOD, GSH-Px, CAT, and content of GSH. Furthermore, the pretreatment of islets with CA returned the changes. These findings indicated that MWCNTs might induce the oxidative stress of pancreatic islets occurring diabetes and protective CA effects that were mediated by the augmentation of the antioxidant defense system of islets. Our research suggested the necessity of conducting further studies on effects of MWCNTs and CA on the diabetes.
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Affiliation(s)
- Akram Ahangarpour
- Diabetes Research Center, Health Research Institute, Department of Physiology, Faculty of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
| | - Soheila Alboghobeish
- Student Research Committee, Department of Pharmacology, School of Pharmacy, Ahvaz Jundishapur University of Medical Sciences, Ahvaz,Iran
| | - Ali Akbar Oroojan
- Student Research Committee, Department of Physiology, Faculty of Medicine, Ahvaz Jundishapur University of Medical Science, Ahvaz, Iran
| | - Mohammad Amin Dehghani
- Student Research Committee, Department of Toxicology, School of Pharmacy, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
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49
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Kyriakides TR, Raj A, Tseng TH, Xiao H, Nguyen R, Mohammed FS, Halder S, Xu M, Wu MJ, Bao S, Sheu WC. Biocompatibility of nanomaterials and their immunological properties. Biomed Mater 2021; 16:10.1088/1748-605X/abe5fa. [PMID: 33578402 PMCID: PMC8357854 DOI: 10.1088/1748-605x/abe5fa] [Citation(s) in RCA: 89] [Impact Index Per Article: 22.3] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Grants] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 08/05/2020] [Accepted: 02/12/2021] [Indexed: 12/16/2022]
Abstract
Nanomaterials (NMs) have revolutionized multiple aspects of medicine by enabling novel sensing, diagnostic, and therapeutic approaches. Advancements in processing and fabrication have also allowed significant expansion in the applications of the major classes of NMs based on polymer, metal/metal oxide, carbon, liposome, or multi-scale macro-nano bulk materials. Concomitantly, concerns regarding the nanotoxicity and overall biocompatibility of NMs have been raised. These involve putative negative effects on both patients and those subjected to occupational exposure during manufacturing. In this review, we describe the current state of testing of NMs including those that are in clinical use, in clinical trials, or under development. We also discuss the cellular and molecular interactions that dictate their toxicity and biocompatibility. Specifically, we focus on the reciprocal interactions between NMs and host proteins, lipids, and sugars and how these induce responses in immune and other cell types leading to topical and/or systemic effects.
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Affiliation(s)
- Themis R Kyriakides
- Department of Biomedical Engineering, Yale University, New Haven, CT 06405, United States of America
- Department of Pathology, Yale University, New Haven, CT 06405, United States of America
- Vascular Biology and Therapeutics Program, Yale University, New Haven, CT 06405, United States of America
| | - Arindam Raj
- Department of Mechanical Engineering and Materials Science, Yale University, New Haven, CT 06405, United States of America
| | - Tiffany H Tseng
- Department of Pathology, Yale University, New Haven, CT 06405, United States of America
- Vascular Biology and Therapeutics Program, Yale University, New Haven, CT 06405, United States of America
| | - Hugh Xiao
- Department of Biomedical Engineering, Yale University, New Haven, CT 06405, United States of America
| | - Ryan Nguyen
- Department of Biomedical Engineering, Yale University, New Haven, CT 06405, United States of America
| | - Farrah S Mohammed
- Department of Biomedical Engineering, Yale University, New Haven, CT 06405, United States of America
| | - Saiti Halder
- Department of Biomedical Engineering, Yale University, New Haven, CT 06405, United States of America
| | - Mengqing Xu
- Department of Biomedical Engineering, Yale University, New Haven, CT 06405, United States of America
- Vascular Biology and Therapeutics Program, Yale University, New Haven, CT 06405, United States of America
| | - Michelle J Wu
- Department of Biomedical Engineering, Yale University, New Haven, CT 06405, United States of America
| | - Shuozhen Bao
- Department of Biomedical Engineering, Yale University, New Haven, CT 06405, United States of America
- Vascular Biology and Therapeutics Program, Yale University, New Haven, CT 06405, United States of America
| | - Wendy C Sheu
- Department of Biomedical Engineering, Yale University, New Haven, CT 06405, United States of America
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50
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Ximendes E, Benayas A, Jaque D, Marin R. Quo Vadis, Nanoparticle-Enabled In Vivo Fluorescence Imaging? ACS NANO 2021; 15:1917-1941. [PMID: 33465306 DOI: 10.1021/acsnano.0c08349] [Citation(s) in RCA: 25] [Impact Index Per Article: 6.3] [Reference Citation Analysis] [Abstract] [MESH Headings] [Track Full Text] [Subscribe] [Scholar Register] [Indexed: 05/21/2023]
Abstract
The exciting advancements that we are currently witnessing in terms of novel materials and synthesis approaches are leading to the development of colloidal nanoparticles (NPs) with increasingly greater tunable properties. We have now reached a point where it is possible to synthesize colloidal NPs with functionalities tailored to specific societal demands. The impact of this new wave of colloidal NPs has been especially important in the field of biomedicine. In that vein, luminescent NPs with improved brightness and near-infrared working capabilities have turned out to be optimal optical probes that are capable of fast and high-resolution in vivo imaging. However, luminescent NPs have thus far only reached a limited portion of their potential. Although we believe that the best is yet to come, the future might not be as bright as some of us think (and have hoped!). In particular, translation of NP-based fluorescence imaging from preclinical studies to clinics is not straightforward. In this Perspective, we provide a critical assessment and highlight promising research avenues based on the latest advances in the fields of luminescent NPs and imaging technologies. The disillusioned outlook we proffer herein might sound pessimistic at first, but we consider it necessary to avoid pursuing "pipe dreams" and redirect the efforts toward achievable-yet ambitious-goals.
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Affiliation(s)
- Erving Ximendes
- Fluorescence Imaging Group, Departamento de Fısica de Materiales, Facultad de Ciencias, Universidad Autónoma de Madrid, C/Francisco Tomás y Valiente 7, Madrid 28049, Spain
- Nanobiology Group, Instituto Ramón y Cajal de Investigación Sanitaria (IRYCIS), Ctra. Colmenar km. 9.100, Madrid 28034, Spain
| | - Antonio Benayas
- Fluorescence Imaging Group, Departamento de Fısica de Materiales, Facultad de Ciencias, Universidad Autónoma de Madrid, C/Francisco Tomás y Valiente 7, Madrid 28049, Spain
- Nanobiology Group, Instituto Ramón y Cajal de Investigación Sanitaria (IRYCIS), Ctra. Colmenar km. 9.100, Madrid 28034, Spain
| | - Daniel Jaque
- Fluorescence Imaging Group, Departamento de Fısica de Materiales, Facultad de Ciencias, Universidad Autónoma de Madrid, C/Francisco Tomás y Valiente 7, Madrid 28049, Spain
- Nanobiology Group, Instituto Ramón y Cajal de Investigación Sanitaria (IRYCIS), Ctra. Colmenar km. 9.100, Madrid 28034, Spain
| | - Riccardo Marin
- Fluorescence Imaging Group, Departamento de Fısica de Materiales, Facultad de Ciencias, Universidad Autónoma de Madrid, C/Francisco Tomás y Valiente 7, Madrid 28049, Spain
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