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Cock IE, Matthews B, Basson AE. Liquid Chromatography-Mass Spectrometry Metabolomic Analysis of Terminalia ferdinandiana Exell. Fruit Extracts That Inhibit HIV-1 Cell Infection, HIV-1 Reverse Transcriptase and HIV-1 Protease. Molecules 2025; 30:1701. [PMID: 40333606 PMCID: PMC12029459 DOI: 10.3390/molecules30081701] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 03/17/2025] [Revised: 04/07/2025] [Accepted: 04/08/2025] [Indexed: 05/09/2025] Open
Abstract
The emergence of HIV strains resistant to the current anti-retroviral drugs has necessitated the search for new anti-retroviral medications. Methanolic and aqueous T. ferdinandiana fruit extracts have potent inhibitory activity against several phases of the HIV-1 replicative cycle. Cell infectivity studies using a non-resistant HIV-1 pseudovirus demonstrated that the methanolic (IC50 16 µg/mL) and aqueous extracts (IC50 19 µg/mL) were potent inhibitors of viral infection in a non-replicating HIV-1 assay. Both extracts also inhibited HIV-1 reverse transcriptase (IC50 values of 35 and 33 µg/mL for methanolic and aqueous extracts, respectively) and HIV-1 protease (IC50 values of 19 and 27 µg/mL, respectively) in recombinant enzyme assays. Given their inhibitory activities against multiple phases of HIV-1 replication, T. ferdinandiana fruit extracts may be particularly useful as HIV-1 therapeutics. Furthermore, both extracts displayed good safety profiles and therapeutic indices, indicating their suitability for therapeutic usage. LC-MS metabolomic profiling analysis of the methanolic extract identified several interesting constituents, including a relative abundance of tannins, as well as several flavonoids and stilbenes. All of these compounds have previously been reported to have bioactivities consistent with the anti-HIV-1 activities reported herein. Based on these studies, methanolic and aqueous T. ferdinandiana fruit extracts are promising potential therapies for the prevention, treatment and management of HIV-1.
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Affiliation(s)
- Ian Edwin Cock
- Centre for Planetary Health and Food Security, Griffith University, Brisbane 4222, Australia
| | - Benjamin Matthews
- Queensland Brain Institute, University of Queensland, Brisbane 4072, Australia;
| | - Adriaan Erasmus Basson
- Department of Molecular Medicine and Haematology, School of Pathology, Faculty of Health Sciences, University of the Witwatersrand, Parktown, Johannesburg 2193, South Africa;
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Sipping M, Kumar TS, Kamdem N. Scientific investigation on antibacterial, antioxidant, cytotoxic effects and TLC bioautography of Terminalia schimperiania stem bark extracts. JOURNAL OF COMPLEMENTARY & INTEGRATIVE MEDICINE 2025; 22:114-123. [PMID: 39789714 DOI: 10.1515/jcim-2024-0251] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Subscribe] [Scholar Register] [Received: 08/03/2024] [Accepted: 11/08/2024] [Indexed: 01/12/2025]
Abstract
OBJECTIVES Terminalia schimperiana Hochst, belonging to the Combretaceae family, is known for its ethnomedicinal values, particularly in treating various diseases in Africa. This study aimed to investigate the antibacterial, antioxidant, and cytotoxic properties of T. schimperiana stem bark extracts, with a specific focus on assessing their bioactive potential and identifying active compounds via TLC bioautography. METHODS The in vitro antimicrobial activity was assessed using the agar well diffusion method against selected clinical strains. Antioxidant activity was evaluated using several methods including free radical scavenging, ferrous ion chelation assays and total phenolic content analysis. The cytotoxicity of the extracts was assessed using MTT assay towards Raw 264.7 and Vero cell lines. RESULTS All extracts demonstrated significant antibacterial activity against the bacteria tested, with inhibition zones (IZ) ranging from 6.50 ± 0.71 to 15.50 ± 0.71 mm and minimum inhibitory concentrations (MIC) ranging from 1.95 to 1,250 μg/mL. The hydroethanolic extract exhibited strongest antioxidant activities with EC50 values of 188.50; 245.30, and 281.50 μg/mL for DPPH; ABTS, ferrous ion chelation assays respectively, and a high content of phenolic compounds (101.67 ± 2.97 µgEFA/mg DW). Importantly, no cytotoxic effects were observed on Raw 264.7 and Vero cell lines. HPTLC analysis identified alkaloids and phenolic compounds in both aqueous and hydroethanolic extracts. CONCLUSIONS These findings indicate T. schimperiana provides a wealth of bio-compounds that can be utilised in the pharmaceutical industry as antibacterial and antioxidant agents to combat antibiotic resistance.
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Affiliation(s)
- Marius Sipping
- PKFokam Institute of Excellence, Yaoundé, Cameroon
- Laboratory of Phytoprotection and Valorization of Genetic Resources, Biotechnology Centre, Nkolbisson, 201336 University of Yaoundé 1 , Yaoundé, Cameroon
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Munengwa A, Nyahangare ET, Jambwa P, Mugoti A, Mandara S, McGaw LJ. Ethnoveterinary medicines used by smallholder farmers for treatment of goat ailments in Chikomba, Murewa, Gutu and Mwenezi districts of Zimbabwe: is there cultural consensus in use practices? JOURNAL OF ETHNOPHARMACOLOGY 2025; 342:119324. [PMID: 39855433 DOI: 10.1016/j.jep.2025.119324] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Subscribe] [Scholar Register] [Received: 04/03/2024] [Revised: 12/31/2024] [Accepted: 01/03/2025] [Indexed: 01/27/2025]
Abstract
ETHNOPHARMACOLOGICAL RELEVANCE Zimbabwe is a key biodiversity domain in sub-Saharan Africa and ethnoveterinary medicines play an integral role in livestock health. However, knowledge on whether plants are used by only a small proportion of people or whether similar uses exist in different communities and in a more regional context is incompletely documented. AIM OF THE STUDY Firstly, the study documented plant-based complementary medicines used for managing goat ailments. Secondly, culturally important medicinal plant species with highest use-reports (UR) as well as botanical and therapeutic consistency were computed. Thirdly, details on whether similar ethnobotanical practices exist in different communities as an indicator of information exchange were explored. MATERIALS AND METHODS A total of 200 informants from Gutu, Chikomba, Murewa and Mwenezi districts of Zimbabwe were interviewed. Plant identification was done at the National Herbarium and Botanic Gardens of Zimbabwe. Use-reports were generated and subjected to analysis using Analysis of Variance (ANOVA) in IBM SPSS statistical software. RESULTS The impact of parasites and diseases was ranked as the major goat production constraint. A total of 160 homemade remedies were documented, 151 of which comprised a single plant species (Homemade Single Species Herbal Remedy Reports, HSHR). The 151 HSHR prepared using 75 plant species belonging to 34 families referred to 401 UR. The foremost used medicinal plant species were from the Fabaceae family (28 HSHR, 19%). Cassia abbreviata was the most cited plant species (6 HSHR, 4%). The category of ecto- and endoparasites had the highest number of UR out of a total of 9 categories (136 UR, 34%). The most frequently used plant species for this category were Solanum campylacanthum (8 UR, 6%) and Strychnos spinosa (6 UR, 4%). Almost similar numbers of UR were computed across communities for 6 disease categories. Nine different combinations (9 HR, 9 UR) comprising mostly of three plant species were reported for various ailments. Baccharoides adoensis and Terminalia sericea were often reported in these combinations for January disease. Highest levels of therapeutic consistency were computed for Aloe vera and Lippia javanica against ectoparasites (10 out of 24 local studies). Interestingly, 28 new medicinal plant species were documented for the first time in Zimbabwe. Again, these were mostly used for ecto-and endoparasites (8 plant species, 29%). CONCLUSIONS Ethnoveterinary medicines play a pivotal role in management of livestock ailments in rural communities of Zimbabwe. However, attention is warranted to ensure transparency in use practices, as well as to conserve and scientifically validate culturally important medicinal plant species such as Aloe vera and Lippia javanica.
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Affiliation(s)
- Anderson Munengwa
- Phytomedicine Programme, Department of Paraclinical Sciences, Faculty of Veterinary Science, University of Pretoria, Private Bag X04, Onderstepoort, 0110, Pretoria, South Africa; Department of Animal Production Sciences, Faculty of Plant and Animal Science and Technology, Marondera University of Agricultural Sciences and Technology, P O Box 35, Marondera, Zimbabwe.
| | - Emmanuel T Nyahangare
- Department of Livestock Sciences, Faculty of Agriculture Environment and Food Systems, University of Zimbabwe, P O Box MP167, Mt Pleasant, Harare, Zimbabwe.
| | - Prosper Jambwa
- Department of Veterinary Biosciences, Faculty of Veterinary Science, University of Zimbabwe, P O Box MP167, Mt Pleasant, Harare, Zimbabwe.
| | - Alban Mugoti
- Department of Animal Production Sciences, Faculty of Plant and Animal Science and Technology, Marondera University of Agricultural Sciences and Technology, P O Box 35, Marondera, Zimbabwe.
| | - Steven Mandara
- Department of Animal Production Sciences, Faculty of Plant and Animal Science and Technology, Marondera University of Agricultural Sciences and Technology, P O Box 35, Marondera, Zimbabwe.
| | - Lyndy J McGaw
- Phytomedicine Programme, Department of Paraclinical Sciences, Faculty of Veterinary Science, University of Pretoria, Private Bag X04, Onderstepoort, 0110, Pretoria, South Africa.
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Ba A, Roumy V, Hughes K, Samaillie J, Bourlet M, Hennebelle T, Ndoye SF, Herent MF, Quetin-Leclercq J, Seck M, Rivière C. Antiparasitic Activities of Plants From the Traditional Senegalese Pharmacopoeia: Isolation of Antitrypanosomal and Antileishmanial Ellagic Acid Derivatives From Terminalia Avicennioides. Chem Biodivers 2025:e202403320. [PMID: 39823173 DOI: 10.1002/cbdv.202403320] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Grants] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 12/14/2024] [Revised: 01/15/2025] [Accepted: 01/16/2025] [Indexed: 01/19/2025]
Abstract
Parasitic diseases such as trypanosomiasis and leishmaniasis pose significant health challenges in Africa. The Senegalese Pharmacopoeia, known for its many medicinal plants with anti-infectious properties, can be a source of antiparasitic natural products. This study aimed to evaluate the in vitro antiparasitic activities of 33 methanolic extracts from 24 ethnopharmacologically selected plants against Trypanosoma brucei brucei (Tbb) and Leishmania mexicana mexicana (Lmm), as well as their cytotoxic activities on WI-38 cells. The most promising plant species were Balanites aegyptiaca, Eucalyptus camaldulensis, Vachellia nilotica and four Combretaceae species. In a second step, antitrypanosomal and antileishmanial compounds were isolated by preparative high-performance liquid chromatography from one of the most active plant extracts, Terminalia avicennioides leaves. Four ellagic acid derivatives were identified by nuclear magnetic resonance (1D & 2D) and high-resolution electrospray ionization-tandem mass spectrometry (HR-ESI-MS/MS) as 3,4,3-tri-O-methylellagic acid (2), 3,4,5-O-trimethyl-3',4'-O,O-methylideneflavellagic acid (3), hexamethylcoruleoellagic acid (4) and pentamethylflavellagic acid (5). In addition, HR-ESI-MS/MS-based dereplication analysis was performed to putatively identify compounds in active cyclohexane and dichloromethane sub-extracts. Pentamethylflavellagic acid (5) showed the most promising activity against Tbb (half-maximal inhibitory concentration = 6.20 µM). This is the first time that these compounds have been isolated from T. avicennioides and evaluated for their antitrypanosomal and antileishmanial activities.
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Affiliation(s)
- Abda Ba
- Joint Research Unit 1158 BioEcoAgro, Univ. Lille, Junia, INRAE, Univ. Liège, UPJV, Univ. Artois, ULCO, Lille, France
- Laboratoire de Chimie Organique et Thérapeutique, Faculté de Médecine, de Pharmacie et d'Odontologie de l'Université Cheikh Anta Diop de Dakar, Dakar-Fann, Senegal
| | - Vincent Roumy
- Joint Research Unit 1158 BioEcoAgro, Univ. Lille, Junia, INRAE, Univ. Liège, UPJV, Univ. Artois, ULCO, Lille, France
| | - Kristelle Hughes
- Pharmacognosy Research Group, Louvain Drug Research Institute, Université Catholique de Louvain (UCLouvain), Brussels, Belgium
| | - Jennifer Samaillie
- Joint Research Unit 1158 BioEcoAgro, Univ. Lille, Junia, INRAE, Univ. Liège, UPJV, Univ. Artois, ULCO, Lille, France
| | - Maude Bourlet
- Pharmacognosy Research Group, Louvain Drug Research Institute, Université Catholique de Louvain (UCLouvain), Brussels, Belgium
| | - Thierry Hennebelle
- Joint Research Unit 1158 BioEcoAgro, Univ. Lille, Junia, INRAE, Univ. Liège, UPJV, Univ. Artois, ULCO, Lille, France
| | - Samba Fama Ndoye
- Laboratoire de Chimie Organique et Thérapeutique, Faculté de Médecine, de Pharmacie et d'Odontologie de l'Université Cheikh Anta Diop de Dakar, Dakar-Fann, Senegal
| | - Marie-France Herent
- Pharmacognosy Research Group, Louvain Drug Research Institute, Université Catholique de Louvain (UCLouvain), Brussels, Belgium
| | - Joëlle Quetin-Leclercq
- Pharmacognosy Research Group, Louvain Drug Research Institute, Université Catholique de Louvain (UCLouvain), Brussels, Belgium
| | - Matar Seck
- Laboratoire de Chimie Organique et Thérapeutique, Faculté de Médecine, de Pharmacie et d'Odontologie de l'Université Cheikh Anta Diop de Dakar, Dakar-Fann, Senegal
| | - Céline Rivière
- Joint Research Unit 1158 BioEcoAgro, Univ. Lille, Junia, INRAE, Univ. Liège, UPJV, Univ. Artois, ULCO, Lille, France
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Agrawal OD, Kulkarni YA. Terminalia catappa aqueous extract reduces hyperglycaemia and oxidative stress in diabetic-hypercholesterolemic rats. J Ayurveda Integr Med 2025; 16:101025. [PMID: 39827649 PMCID: PMC11787580 DOI: 10.1016/j.jaim.2024.101025] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 03/14/2024] [Revised: 06/22/2024] [Accepted: 06/23/2024] [Indexed: 01/22/2025] Open
Abstract
BACKGROUND Terminalia catappa is an important medicinal plant. Plants from the genus Terminalia have been reported for antidiabetic effects. OBJECTIVE To study effect of Terminalia catappa leaves aqueous extract in type 2 diabetic rats. MATERIALS AND METHODS High-fat diet (HFD) and a low dose of streptozotocin (35 mg/kg, i.p.) were used for inducing type 2 diabetes in rats; streptozotocin was injected after two weeks of dietary modification with HFD. HFD was continued throughout the study. Doses of 500 and 1000 mg/kg of aqueous extract of Terminalia catappa were used as a treatment for six weeks. At the end of the study, biochemical and oxidative stress parameters were estimated. Histopathology and immunohistochemistry of pancreatic tissue were performed. RESULTS Significant decrease (p < 0.01, p < 0.001) was observed in glucose levels of diabetic animals treated with T. catappa extract at both dose levels when compared with diabetic control group. Treatment with aqueous extract reduced cholesterol levels significantly (p < 0.05). After receiving 1000 mg/kg of extract, the triglyceride level was significantly lowered (p < 0.01) in the diabetic rats. Extract treatment enhanced the insulin sensitivity index (ISI), while insulin levels and HOMA-IR were markedly reduced. Treatment with 1000 mg/kg of extract significantly enhanced the levels of GSH and catalase. Histopathological analysis of the pancreas showed that damage caused by hyperglycemia was prevented in the extract-treated groups. Pancreatic tissue showed an increased expression of SIRT1 in extract-treated animals. CONCLUSION The current study's findings prove that the extract has remarkable antidiabetic effects.
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Affiliation(s)
- Ojaskumar D Agrawal
- Shobhaben Pratapbhai Patel School of Pharmacy & Technology Management, SVKM's NMIMS, V.L. Mehta Road, Vile Parle (W), Mumbai, 400 056, India; Vivekanand Education Society's College of Pharmacy, Chembur (E), University of Mumbai, Mumbai 400 074, India
| | - Yogesh A Kulkarni
- Shobhaben Pratapbhai Patel School of Pharmacy & Technology Management, SVKM's NMIMS, V.L. Mehta Road, Vile Parle (W), Mumbai, 400 056, India.
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Tiwana G, Cock IE, Cheesman MJ. Phytochemical Analysis and Antimicrobial Activity of Terminalia bellirica (Gaertn.) Roxb. and Terminalia chebula Retz. Fruit Extracts Against Gastrointestinal Pathogens: Enhancing Antibiotic Efficacy. Microorganisms 2024; 12:2664. [PMID: 39770866 PMCID: PMC11728670 DOI: 10.3390/microorganisms12122664] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 10/23/2024] [Revised: 12/12/2024] [Accepted: 12/20/2024] [Indexed: 01/16/2025] Open
Abstract
Terminalia bellirica (Gaertn) Roxb. and Terminalia chebula Retz. are significant botanicals in ancient Ayurvedic medicine. They are renowned for their therapeutic properties, notably in addressing gastrointestinal (GI) diseases. These plants have undergone thorough examination related to their antibacterial, anti-inflammatory, and antioxidant properties, which make them highly efficient natural treatments for controlling gastrointestinal infections. The current research demonstrated the antibacterial efficacy of fruit extracts of Terminalia bellirica and Terminalia chebula against Bacillus cereus, Shigella sonnei, Shigella flexneri, and Salmonella typhimurium. We performed disc diffusion and liquid microdilution experiments to evaluate the antibacterial efficacy. All extracts of Terminalia bellirica and Terminalia chebula showed good antibacterial effects against B. cereus and S. flexneri. The minimum inhibitory concentration (MIC) values ranged from 94 µg/mL to 556 µg/mL. The methanolic extracts from both plants also showed noteworthy antibacterial activity against S. sonnei and S. typhimurium, with MIC values of 755 µg/mL for both. Fractional inhibitory concentration studies revealed additive interactions between some conventional antibiotics and the plant extracts when used concurrently. Liquid chromatography-mass spectrometry (LC-MS) analyses revealed that the T. bellirica and T. chebula extracts contained various tannins including methyl gallate, propyl gallate, gallic acid, and ellagic acid. Lethality assays conducted using Artemia franciscana Kellogg nauplii indicated that all the plant extracts are non-toxic. The antibacterial properties and absence of toxicity in T. bellirica and T. chebula fruit extracts indicate their potential for antibiotic development, warranting additional mechanistic and phytochemical studies.
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Affiliation(s)
- Gagan Tiwana
- School of Pharmacy and Medical Sciences, Gold Coast Campus, Griffith University, Gold Coast 4222, Australia;
| | - Ian Edwin Cock
- School of Environment and Science, Nathan Campus, Griffith University, Brisbane 4111, Australia;
| | - Matthew James Cheesman
- School of Pharmacy and Medical Sciences, Gold Coast Campus, Griffith University, Gold Coast 4222, Australia;
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Tiwana G, Cock IE, Cheesman MJ. Combinations of Terminalia bellirica (Gaertn.) Roxb. and Terminalia chebula Retz. Extracts with Selected Antibiotics Against Antibiotic-Resistant Bacteria: Bioactivity and Phytochemistry. Antibiotics (Basel) 2024; 13:994. [PMID: 39452260 PMCID: PMC11504310 DOI: 10.3390/antibiotics13100994] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 09/19/2024] [Revised: 10/16/2024] [Accepted: 10/18/2024] [Indexed: 10/26/2024] Open
Abstract
Antimicrobial resistance (AMR) has arisen due to antibiotic overuse and misuse. Antibiotic resistance renders standard treatments less effective, making it difficult to control some infections, thereby increasing morbidity and mortality. Medicinal plants are attracting increased interest as antibiotics lose efficacy. This study evaluates the antibacterial activity of solvent extracts prepared using Terminalia bellirica and Terminalia chebula fruit against six bacterial pathogens using disc diffusion and broth microdilution assays. The aqueous and methanol extracts of T. bellirica and T. chebula showed substantial zones of inhibition (ZOIs) against Staphylococcus aureus and methicillin-resistant S. aureus (MRSA). The activity against those bacteria was strong, with minimum inhibitory concentrations (MIC) ranging from 94 µg/mL to 392 µg/mL. Additionally, the T. bellirica methanolic extract showed noteworthy antibacterial activity against Escherichia coli and an extended spectrum β-lactamase (ESBL) E. coli strain (MIC values of 755 µg/mL for both). The aqueous T. bellirica and T. chebula extracts also inhibited Klebsiella pneumoniae growth (MIC values of 784 µg/mL and 556 µg/mL, respectively). The corresponding methanolic extracts also inhibited ESBL K. pneumoniae growth (MIC values of 755 µg/mL and 1509 µg/mL, respectively). Eighteen additive interactions were observed when extracts were combined with reference antibiotics. Strong antagonism occurred when any of the extracts were mixed with polymyxin B. Liquid chromatography-mass spectroscopy (LC-MS) analysis of the extracts revealed several interesting flavonoids and tannins, including 6-galloylglucose, 1,2,6-trigalloyl-β-D-glucopyranose, 6-O-[(2E)-3-phenyl-2-propenoyl]-1-O-(3,4,5-trihydroxybenzoyl)-β-D-glucopyranose, propyl gallate, methyl gallate, sanguiin H4, hamamelitannin, pyrogallol, gallic acid, ellagic acid, chebulic acid, and chebuloside II. All extracts were nontoxic in brine shrimp assays. This lack of toxicity, combined with their antibacterial activities, suggests that these plant species may be promising sources of antibacterial compound(s) that warrant further study.
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Affiliation(s)
- Gagan Tiwana
- School of Pharmacy and Medical Sciences, Gold Coast Campus, Griffith University, Gold Coast 4222, Australia;
| | - Ian Edwin Cock
- School of Environment and Science, Nathan Campus, Griffith University, Brisbane 4111, Australia;
| | - Matthew James Cheesman
- School of Pharmacy and Medical Sciences, Gold Coast Campus, Griffith University, Gold Coast 4222, Australia;
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Shreevatsa B, Hegde S, Narayan P, Dharmashekar C, Jain A, Wani TA, Prabhuswamimath SC, Kollur SP, Shivamallu C. Targeting FAK, VEGF, and MTA1 proteins with Terminalia elliptica: a computational approach for anticancer activity. Front Oncol 2024; 14:1427632. [PMID: 39355129 PMCID: PMC11442428 DOI: 10.3389/fonc.2024.1427632] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 05/07/2024] [Accepted: 08/20/2024] [Indexed: 10/03/2024] Open
Abstract
Cancer remains a significant global health challenge, prompting exploration into alternative treatments such as those derived from natural compounds found in traditional medicine. Recent research has underscored the role of proteins like Focal Adhesion Kinase (FAK), Vascular Endothelial Growth Factor (VEGF), and Metastasis-Associated Protein 1 (MTA1) in driving cancer cell proliferation and survival. Here, we investigated the potential of a single molecule to modulate these key proteins involved in metastasis, offering a promising avenue for cancer therapy. Terminalia elliptica, commonly known as Asna, possesses a diverse range of medicinal properties, including antimicrobial, anti-inflammatory, anticancer, antidiabetic, antiaging, hepatoprotective, antioxidant, and neuroprotective activities. Our study aimed to explore the anticancer potential of Terminalia elliptica by identifying bioactive compounds capable of targeting FAK, VEGF, and MTA1 to impede cancer metastasis. Through in silico analysis, we conducted network analysis using Cytoscape to assess the significance of these bioactive compounds in the inhibition of signaling pathways driving metastasis. The utilization of these bioactives as potential candidates for targeted therapy of VEGF, FAK, and MTA1 regulated pathways was preliminarily assessed by Molecular Docking and MD Simulation. Our findings revealed that phytobioactives namely, Chebulinic Acid of Terminalia elliptica, exhibited notable binding affinity and interaction with FAK, and Chebulagic Acid with VEGF, and MTA1. This discovery holds promise as a novel therapeutic approach for combating cancer, offering potential benefits in cancer treatment and management.
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Affiliation(s)
- Bhargav Shreevatsa
- Department of Biotechnology and Bioinformatics, JSS Academy of Higher Education and Research, Mysuru, Karnataka, India
| | - Shrivatsa Hegde
- Department of Biotechnology and Bioinformatics, JSS Academy of Higher Education and Research, Mysuru, Karnataka, India
| | - Prakruthi Narayan
- Department of Biotechnology and Bioinformatics, JSS Academy of Higher Education and Research, Mysuru, Karnataka, India
| | - Chandan Dharmashekar
- Department of Biotechnology and Bioinformatics, JSS Academy of Higher Education and Research, Mysuru, Karnataka, India
| | - Anisha Jain
- Department of Microbiology, JSS Academy of Higher Education and Research, Mysuru, Karnataka, India
| | - Tanveer A Wani
- Department of Pharmaceutical Chemistry, College of Pharmacy, King Saud University, Riyadh, Saudi Arabia
| | - Samudyata C Prabhuswamimath
- Department of Biotechnology and Bioinformatics, JSS Academy of Higher Education and Research, Mysuru, Karnataka, India
| | - Shiva Prasad Kollur
- School of Physical Sciences, Amrita Vishwa Vidyapeetham, Mysuru, Karnataka, India
| | - Chandan Shivamallu
- Department of Biotechnology and Bioinformatics, JSS Academy of Higher Education and Research, Mysuru, Karnataka, India
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Ba A, Roumy V, Al Ibrahim M, Raczkiewicz I, Samaillie J, Hakem A, Sahpaz S, Belouzard S, Diatta W, Sidybé M, Neut C, Séron K, Seck M, Rivière C. Antibacterial and anti-coronavirus investigation of selected Senegalese plant species according to an ethnobotanical survey. JOURNAL OF ETHNOPHARMACOLOGY 2024; 328:118070. [PMID: 38521430 DOI: 10.1016/j.jep.2024.118070] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Subscribe] [Scholar Register] [Received: 09/23/2023] [Revised: 02/28/2024] [Accepted: 03/17/2024] [Indexed: 03/25/2024]
Abstract
ETHNOPHARMACOLOGICAL RELEVANCE In Senegal, upper and lower respiratory tract infections constitute a real health problem. To manage these disorders, most people rely on the use of local medicinal plants. This is particularly the case for species belonging to the botanical families, Combretaceae, Fabaceae, Myrtaceae and Rubiaceae, which are widely used to treat various respiratory problems such as colds, flu, rhinitis, sinusitis, otitis, angina, bronchitis, bronchiolitis and also pneumonia. AIM OF THE STUDY The aim of this study was to identify medicinal plants traditionally used for the management of infectious diseases, in particular those of the respiratory tract. On the basis of these ethnopharmacological uses, this study made it possible to highlight the antibacterial, antiviral and cytotoxic activities of selected plant species. MATERIALS AND METHODS An ethnobotanical survey was conducted in Senegal among informants, including herbalists, traditional healers, and households, using medicinal plants in the management of infectious diseases, with a focus on respiratory tract infections. The most cited plant species were evaluated in vitro on a panel of 18 human pathogenic bacteria may be involved in respiratory infections and against the human coronavirus HCoV-229E in Huh-7 cells. The antiviral activity of the most active extracts against HCoV-229E was also evaluated on COVID-19 causing agent, SARS-CoV-2 in Vero-81 cells. In parallel, cytotoxic activities were evaluated on Huh-7 cells. RESULTS A total of 127 informants, including 100 men (78.74%) and 27 women (21.26%) participated in this study. The ethnobotanical survey led to the inventory of 41 plant species belonging to 19 botanical families used by herbalists and/or traditional healers and some households to treat infectious diseases, with a specific focus on upper respiratory tract disorders. Among the 41 plant species, the most frequently mentioned in the survey were Guiera senegalensis J.F. Gmel. (95.2%), Combretum glutinosum Perr. Ex DC. (93.9%) and Eucalyptus spp. (82.8%). Combretaceae (30.2%) represented the most cited botanical family with six species, followed by Fabaceae (29.3%, 12 species). A total of 33 crude methanolic extracts of the 24 plant species selected for their number of citations were evaluated in vitro for their antimicrobial and cytotoxic activities. Guiera senegalensis, Combretum glutinosum, Vachellia nilotica subsp. tomentosa (Benth.) Kyal. & Boatwr, Eucalyptus camaldulensis Dehnh., and Terminalia avicennioides Guill. & Perr., showed antibacterial activities. The most active plants against HCoV-229E were: Ficus sycomorus L., Mitragyna inermis (Willd.) Kuntze, Pterocarpus erinaceus Poir., and Spermacoce verticillata L. One of these plants, Mitragyna inermis, was also active against SARS-CoV-2. CONCLUSION This work confirmed the anti-infective properties of plant species traditionally used in Senegal. Overall, the most frequently cited plant species showed the best antibacterial activities. Moreover, some of the selected plant species could be considered as a potential source for the management of coronavirus infections. This new scientific data justified the use of these plants in the management of some infectious pathologies, especially those of the respiratory tract.
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Affiliation(s)
- Abda Ba
- Joint Research Unit 1158 BioEcoAgro, Univ. Lille, Junia, INRAE, Univ. Liège, UPJV, Univ. Artois, ULCO, F-59650, Villeneuve d'Ascq, France; Laboratoire de Chimie Organique et Thérapeutique, Faculté de Médecine, de Pharmacie et D'Odontologie de l'Université Cheikh Anta Diop de Dakar, BP 5005, Dakar-Fann, Senegal
| | - Vincent Roumy
- Joint Research Unit 1158 BioEcoAgro, Univ. Lille, Junia, INRAE, Univ. Liège, UPJV, Univ. Artois, ULCO, F-59650, Villeneuve d'Ascq, France
| | - Malak Al Ibrahim
- Joint Research Unit 1158 BioEcoAgro, Univ. Lille, Junia, INRAE, Univ. Liège, UPJV, Univ. Artois, ULCO, F-59650, Villeneuve d'Ascq, France; Univ. Lille, CNRS, Inserm, CHU Lille, Institut Pasteur de Lille, U1019 - UMR9017 - Center for Infection and Immunity of Lille (CIIL), F-59000, Lille, France
| | - Imelda Raczkiewicz
- Joint Research Unit 1158 BioEcoAgro, Univ. Lille, Junia, INRAE, Univ. Liège, UPJV, Univ. Artois, ULCO, F-59650, Villeneuve d'Ascq, France; Univ. Lille, CNRS, Inserm, CHU Lille, Institut Pasteur de Lille, U1019 - UMR9017 - Center for Infection and Immunity of Lille (CIIL), F-59000, Lille, France
| | - Jennifer Samaillie
- Joint Research Unit 1158 BioEcoAgro, Univ. Lille, Junia, INRAE, Univ. Liège, UPJV, Univ. Artois, ULCO, F-59650, Villeneuve d'Ascq, France
| | - Asma Hakem
- Joint Research Unit 1158 BioEcoAgro, Univ. Lille, Junia, INRAE, Univ. Liège, UPJV, Univ. Artois, ULCO, F-59650, Villeneuve d'Ascq, France
| | - Sevser Sahpaz
- Joint Research Unit 1158 BioEcoAgro, Univ. Lille, Junia, INRAE, Univ. Liège, UPJV, Univ. Artois, ULCO, F-59650, Villeneuve d'Ascq, France
| | - Sandrine Belouzard
- Univ. Lille, CNRS, Inserm, CHU Lille, Institut Pasteur de Lille, U1019 - UMR9017 - Center for Infection and Immunity of Lille (CIIL), F-59000, Lille, France
| | - William Diatta
- Laboratoire de Pharmacognosie et Botanique, Faculté de Médecine, de Pharmacie et D'Odontologie de l'Université Cheikh Anta Diop de Dakar, BP 5005, Dakar-Fann, Senegal
| | - Mamadou Sidybé
- Laboratoire de botanique et biodiversité (LBB), Département Biologie Végétale, Faculté des Sciences et Techniques, Université Cheikh Anta Diop de Dakar, BP 5005, Dakar-Fann, Senegal
| | - Christel Neut
- Univ. Lille, INSERM, CHU Lille, U1286 INFINITE, F-59000, Lille, France
| | - Karin Séron
- Univ. Lille, CNRS, Inserm, CHU Lille, Institut Pasteur de Lille, U1019 - UMR9017 - Center for Infection and Immunity of Lille (CIIL), F-59000, Lille, France
| | - Matar Seck
- Laboratoire de Chimie Organique et Thérapeutique, Faculté de Médecine, de Pharmacie et D'Odontologie de l'Université Cheikh Anta Diop de Dakar, BP 5005, Dakar-Fann, Senegal
| | - Céline Rivière
- Joint Research Unit 1158 BioEcoAgro, Univ. Lille, Junia, INRAE, Univ. Liège, UPJV, Univ. Artois, ULCO, F-59650, Villeneuve d'Ascq, France.
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10
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Cock IE. Terminalia ferdinandiana Exell. extracts reduce pro-inflammatory cytokine and PGE 2 secretion, decrease COX-2 expression and down-regulate cytosolic NF-κB levels. Inflammopharmacology 2024; 32:1839-1853. [PMID: 38581641 PMCID: PMC11136772 DOI: 10.1007/s10787-024-01462-7] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Grants] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 01/10/2024] [Accepted: 03/12/2024] [Indexed: 04/08/2024]
Abstract
Based on their high antioxidant capacity and noteworthy phytochemistry, Terminalia ferdinandiana fruit and leaves have attracted considerable recent interest for their therapeutic potential. Whilst those studies have reported a variety of therapeutic properties for the fruit, the anti-inflammatory potential of T. ferdinandiana has been largely neglected and the leaves have been almost completely ignored. This study investigated the immune-modulatory and anti-inflammatory properties of T. ferdinandiana fruit and leaf extracts by evaluating their inhibition of multiple pro- and anti-inflammatory cytokines and chemokines secretion in lipopolysaccharide (LPS)-stimulated and unstimulated RAW 264.7 macrophages using multiplex bead immunoassays and ELISA assays. The methanolic extracts were particularly good immune-modulators, significantly inhibiting the secretion of all the cytokines and chemokines tested. Indeed, the methanolic extracts completely inhibited IL-10, IFN-γ, IL-1β, IL-6, MCP-1, and MIP-2a secretion, and almost completely inhibited the secretion of TNF-α. In addition, the methanolic T. ferdinandiana extracts also significantly inhibited cytosolic COX-2 levels (by 87-95%) and the synthesis of the PGE2 (by ~ 98%). In contrast, the methanolic extracts stimulated LTB4 secretion by ~ 60-90%, whilst the aqueous extracts significantly inhibited LTB4 secretion (by ~ 27% each). Exposure of RAW 264.7 cells to the methanolic T. ferdinandiana extracts also significantly down-regulated the cytosolic levels of NF-κB by 33-44%, indicating that the immune-modulatory and anti-inflammatory properties of the extracts may be regulated via a decrease in NF-κB transcription pathways. Taken together, these results demonstrate potent anti-inflammatory properties for the extracts and provide insights into their anti-inflammatory mechanisms.
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Affiliation(s)
- Ian E Cock
- Centre for Planetary Health and Food Security, Griffith University, Nathan Campus, 170 Kessels Rd, Nathan, QLD, 4111, Australia.
- School of Environment and Science, Griffith University, Nathan Campus, 170 Kessels Rd, Nathan, QLD, 4111, Australia.
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11
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Mani J, Johnson J, Hosking H, Schmidt L, Batley R, du Preez R, Broszczak D, Walsh K, Neilsen P, Naiker M. Bioassay-Guided Fractionation of Pittosporum angustifolium and Terminalia ferdinandiana with Liquid Chromatography Mass Spectroscopy and Gas Chromatography Mass Spectroscopy Exploratory Study. PLANTS (BASEL, SWITZERLAND) 2024; 13:807. [PMID: 38592847 PMCID: PMC10974205 DOI: 10.3390/plants13060807] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Subscribe] [Scholar Register] [Received: 01/13/2024] [Revised: 02/28/2024] [Accepted: 03/06/2024] [Indexed: 04/11/2024]
Abstract
Bioprospecting native Australian plants offers the potential discovery of latent and novel bioactive compounds. The promising cytotoxic and antibacterial activity of methanolic extracts of Pittosporum angustifolium and Terminalia ferdinandiana led to further fractionation and isolation using our laboratory's bioassay-guided fractionation protocol. Hence, the aim of this study was to further evaluate the bioactivity of the fractions and subfractions and characterize bioactive compounds using liquid chromatography mass spectroscopy (LC-MS/MS) and gas chromatography MS (GC-MS). Compounds tentatively identified in P. angustifolium Fraction 1 using LC-ESI-QTOF-MS/MS were chlorogenic acid and/or neochlorogenic acid, bergapten, berberine, 8'-epitanegool and rosmarinic acid. GC-MS analysis data showed the presence of around 100 compounds, mainly comprising carboxylic acids, sugars, sugar alcohols, amino acids and monoalkylglycerols. Furthermore, the fractions obtained from T. ferdinandiana flesh extracts showed no cytotoxicity, except against HT29 cell lines, and only Fraction 2 exhibited some antibacterial activity. The reduced bioactivity observed in the T. ferdinandiana fractions could be attributed to the potential loss of synergy as compounds become separated within the fractions. As a result, the further fractionation and separation of compounds in these samples was not pursued. However, additional dose-dependent studies are warranted to validate the bioactivity of T. ferdinandiana flesh fractions, particularly since this is an understudied species. Moreover, LC-MS/GC-MS studies confirm the presence of bioactive compounds in P. angustifolium Fraction 1/subfractions, which helps to explain the significant acute anticancer activity of this plant. The screening process designed in this study has the potential to pave the way for developing scientifically validated phytochemical/bioactivity information on ethnomedicinal plants, thereby facilitating further bioprospecting efforts and supporting the discovery of novel drugs in modern medicine.
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Affiliation(s)
- Janice Mani
- College of Science and Sustainability, CQUniversity, North Rockhampton, QLD 4701, Australia; (J.J.); (H.H.); (R.B.); (R.d.P.); (K.W.); (P.N.); (M.N.)
- Institute for Future Farming Systems, CQUniversity, Bundaberg, QLD 4670, Australia
| | - Joel Johnson
- College of Science and Sustainability, CQUniversity, North Rockhampton, QLD 4701, Australia; (J.J.); (H.H.); (R.B.); (R.d.P.); (K.W.); (P.N.); (M.N.)
- Institute for Future Farming Systems, CQUniversity, Bundaberg, QLD 4670, Australia
| | - Holly Hosking
- College of Science and Sustainability, CQUniversity, North Rockhampton, QLD 4701, Australia; (J.J.); (H.H.); (R.B.); (R.d.P.); (K.W.); (P.N.); (M.N.)
| | - Luke Schmidt
- School of Biomedical Sciences, Queensland University of Technology, Brisbane, QLD 4000, Australia; (L.S.); (D.B.)
| | - Ryan Batley
- College of Science and Sustainability, CQUniversity, North Rockhampton, QLD 4701, Australia; (J.J.); (H.H.); (R.B.); (R.d.P.); (K.W.); (P.N.); (M.N.)
| | - Ryan du Preez
- College of Science and Sustainability, CQUniversity, North Rockhampton, QLD 4701, Australia; (J.J.); (H.H.); (R.B.); (R.d.P.); (K.W.); (P.N.); (M.N.)
| | - Daniel Broszczak
- School of Biomedical Sciences, Queensland University of Technology, Brisbane, QLD 4000, Australia; (L.S.); (D.B.)
| | - Kerry Walsh
- College of Science and Sustainability, CQUniversity, North Rockhampton, QLD 4701, Australia; (J.J.); (H.H.); (R.B.); (R.d.P.); (K.W.); (P.N.); (M.N.)
- Institute for Future Farming Systems, CQUniversity, Bundaberg, QLD 4670, Australia
| | - Paul Neilsen
- College of Science and Sustainability, CQUniversity, North Rockhampton, QLD 4701, Australia; (J.J.); (H.H.); (R.B.); (R.d.P.); (K.W.); (P.N.); (M.N.)
| | - Mani Naiker
- College of Science and Sustainability, CQUniversity, North Rockhampton, QLD 4701, Australia; (J.J.); (H.H.); (R.B.); (R.d.P.); (K.W.); (P.N.); (M.N.)
- Institute for Future Farming Systems, CQUniversity, Bundaberg, QLD 4670, Australia
- Jawun Research Centre, Cairns, QLD 4870, Australia
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12
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Zai MJ, Cheesman MJ, Cock IE. Terminalia petiolaris A.Cunn ex Benth. Extracts Have Antibacterial Activity and Potentiate Conventional Antibiotics against β-Lactam-Drug-Resistant Bacteria. Antibiotics (Basel) 2023; 12:1643. [PMID: 37998845 PMCID: PMC10669112 DOI: 10.3390/antibiotics12111643] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 10/31/2023] [Revised: 11/15/2023] [Accepted: 11/17/2023] [Indexed: 11/25/2023] Open
Abstract
Terminalia petiolaris A. Cunn. Ex Benth. (genus: Terminalia, family: Combretaceae) is native to Australia. Terminalia spp. have traditionally been used to treat various ailments, including bacterial infections. Solvents of varying polarity were used to extract compounds from leaves of this species, and the extracts were tested against a panel of bacteria, including antibiotic-resistant strains. The methanolic and water extracts showed substantial inhibitory activity against several bacteria, including antibiotic-resistant strains in both disc diffusion and liquid dilution assays. Combining these extracts with selected conventional antibiotics enhanced the inhibition of bacterial growth for some combinations, while others showed no significant interaction. In total, two synergistic, twenty-five additive, twenty-three non-interactive and one antagonistic interaction were observed. The methanolic and ethyl acetate plant extracts were found to be non-toxic in Artemia franciscana nauplii toxicity assays. A liquid chromatography-mass spectrometry metabolomics analysis identified several flavonoid compounds, including miquelianin, trifolin and orientin, which might contribute to the observed activities. The potential modes of these active extracts are further discussed in this study.
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Affiliation(s)
- Muhammad Jawad Zai
- Centre for Planetary Health and Food Security, Griffith University, Brisbane, QLD 4111, Australia; (M.J.Z.); (I.E.C.)
- School of Environment and Science, Griffith University, Brisbane, QLD 4111, Australia
| | - Matthew James Cheesman
- School of Pharmacy and Medical Sciences, Griffith University, Southport, QLD 4222, Australia
| | - Ian Edwin Cock
- Centre for Planetary Health and Food Security, Griffith University, Brisbane, QLD 4111, Australia; (M.J.Z.); (I.E.C.)
- School of Environment and Science, Griffith University, Brisbane, QLD 4111, Australia
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13
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Sultan MT, Anwar MJ, Imran M, Khalil I, Saeed F, Neelum S, Alsagaby SA, Al Abdulmonem W, Abdelgawad MA, Hussain M, El-Ghorab AH, Umar M, Al Jbawi E. Phytochemical profile and pro-healthy properties of
Terminalia chebula
: A comprehensive review. INTERNATIONAL JOURNAL OF FOOD PROPERTIES 2023; 26:526-551. [DOI: 10.1080/10942912.2023.2166951] [Citation(s) in RCA: 5] [Impact Index Per Article: 2.5] [Reference Citation Analysis] [Track Full Text] [Subscribe] [Scholar Register] [Received: 10/03/2022] [Revised: 01/02/2023] [Accepted: 01/05/2023] [Indexed: 02/01/2023]
Affiliation(s)
| | | | - Muhammad Imran
- Department of Food Science and Technology, University of Narowal-Pakistan, Narowal, Pakistan
| | - Ijaz Khalil
- Institute of Food and Nutrition, Bahauddin Zakariya University, Multan, Pakistan
| | - Farhan Saeed
- Department of Food Sciences, Government College University Faisalabad, Faisalabad, Pakistan
| | - Shahzadi Neelum
- Department of Biochemistry, Hamdard University, Karachi, Pakistan
| | - Suliman A. Alsagaby
- Department of Medical Laboratory Sciences, College of Applied Medical Sciences, Majmaah University, AL-Majmaah, Saudi Arabia
| | - Waleed Al Abdulmonem
- Department of Pathology, College of Medicine, Qassim University, Buraidah, Kingdom of Saudi Arabia
| | - Mohamed A. Abdelgawad
- Department of Pharmaceutical Chemistry, College of Pharmacy, Jouf University, Sakaka, Saudi Arabia
| | - Muzzamal Hussain
- Department of Food Sciences, Government College University Faisalabad, Faisalabad, Pakistan
| | - Ahmed H. El-Ghorab
- Department of Chemistry, College of Science, Jouf University, Sakaka, Saudi Arabia
| | - Maryam Umar
- Department of Food Sciences, Government College University Faisalabad, Faisalabad, Pakistan
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14
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Teerasumran P, Velliou E, Bai S, Cai Q. Deodorants and antiperspirants: New trends in their active agents and testing methods. Int J Cosmet Sci 2023; 45:426-443. [PMID: 36896776 PMCID: PMC10946881 DOI: 10.1111/ics.12852] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Grants] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 01/27/2023] [Revised: 02/22/2023] [Accepted: 02/22/2023] [Indexed: 03/11/2023]
Abstract
Sweating is the human body's thermoregulation system but also results in unpleasant body odour which can diminish the self-confidence of people. There has been continued research in finding solutions to reduce both sweating and body odour. Sweating is a result of increased sweat flow and malodour results from certain bacteria and ecological factors such as eating habits. Research on deodorant development focuses on inhibiting the growth of malodour-forming bacteria using antimicrobial agents, whereas research on antiperspirant synthesis focuses on technologies reducing the sweat flow, which not only reduces body odour but also improves people's appearance. Antiperspirant's technology is based on the use of aluminium salts which can form a gel plug at sweat pores, obstructing the sweat fluid from arising onto the skin surface. In this paper, we perform a systematic review on the recent progress in the development of novel antiperspirant and deodorant active ingredients that are alcohol-free, paraben-free, and naturally derived. Several studies have been reported on the alternative class of actives that can potentially be used for antiperspirant and body odour treatment including deodorizing fabric, bacterial, and plant extracts. However, a significant challenge is to understand how the gel-plugs of antiperspirant actives are formed in sweat pores and how to deliver long-lasting antiperspirant and deodorant benefits.
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Affiliation(s)
- Paweenuch Teerasumran
- Department of Chemical and Process Engineering, Faculty of Engineering and Physical SciencesUniversity of SurreyGuildfordGU2 7XHUK
- Centre for 3D Models of Health and DiseaseUCL‐Division of Surgery and Interventional ScienceCharles Bell House, 43‐45 Foley Street, FitzroviaLondonW1W 7TYUK
- The State Key Laboratory of Biochemical Engineering, Institute of Process EngineeringChinese Academy of SciencesBeijing100190China
| | - Eirini Velliou
- Department of Chemical and Process Engineering, Faculty of Engineering and Physical SciencesUniversity of SurreyGuildfordGU2 7XHUK
- Centre for 3D Models of Health and DiseaseUCL‐Division of Surgery and Interventional ScienceCharles Bell House, 43‐45 Foley Street, FitzroviaLondonW1W 7TYUK
| | - Shuo Bai
- The State Key Laboratory of Biochemical Engineering, Institute of Process EngineeringChinese Academy of SciencesBeijing100190China
| | - Qiong Cai
- Department of Chemical and Process Engineering, Faculty of Engineering and Physical SciencesUniversity of SurreyGuildfordGU2 7XHUK
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15
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Hussain S, Javed W, Tajammal A, Khalid M, Rasool N, Riaz M, Shahid M, Ahmad I, Muhammad R, Shah SAA. Synergistic Antibacterial Screening of Cymbopogon citratus and Azadirachta indica: Phytochemical Profiling and Antioxidant and Hemolytic Activities. ACS OMEGA 2023; 8:16600-16611. [PMID: 37214690 PMCID: PMC10193546 DOI: 10.1021/acsomega.2c06785] [Citation(s) in RCA: 4] [Impact Index Per Article: 2.0] [Reference Citation Analysis] [Abstract] [Track Full Text] [Download PDF] [Figures] [Subscribe] [Scholar Register] [Received: 11/04/2022] [Accepted: 03/02/2023] [Indexed: 05/24/2023]
Abstract
Current studies were performed to investigate the phytochemistry, synergistic antibacterial, antioxidant, and hemolytic activities of ethanolic and aqueous extracts of Azadirachta indica (EA and WA) and Cymbopogon citratus (EC and WC) leaves. Fourier transform infrared data verified the existence of alcoholic, carboxylic, aldehydic, phenyl, and bromo moieties in plant leaves. The ethanolic extracts (EA and EC) were significantly richer in phenolics and flavonoids as compared to the aqueous extracts (WA and WC). The ethanolic extract of C. citratus (EC) contained higher concentrations of caffeic acid (1.432 mg/g), synapic acid (6.743 mg/g), and benzoic acid (7.431 mg/g) as compared to all other extracts, whereas chlorogenic acid (0.311 mg/g) was present only in the aqueous extract of A. indica (WA). Food preservative properties of C. citratus can be due to the presence of benzoic acid (7.431 mg/g). -Gas chromatography-mass spectrometry analysis demonstrated the presence of 36 and 23 compounds in A. indica and C. citratus leaves, respectively. Inductively coupled plasma analysis was used to determine the concentration of 26 metals (Al, As, B, Ba, Ca, Cd, Co, Cr, Cu, Fe, K, Mg, Mn, Mo, Na, Ni, Pb, Sb, Se, Si, Sn, Sr, V, Zn, Zr, Ti); the metal concentrations were higher in aqueous extracts as compared to the ethanolic extracts. The extracts were generally richer in calcium (3000-7858 ppm), potassium (13662-53,750 ppm), and sodium (3181-8445 ppm) and hence can be used in food supplements as a source of these metals. Antioxidant potential (DDPH method) of C. citratus ethanolic extract was the highest (74.50 ± 0.66%), whereas it was the lowest (32.22 ± 0.28%) for the aqueous extract of A. indica. Synergistic inhibition of bacteria (Staphylococcus aureus and Escherichia coli) was observed when the aqueous extracts of both the plants were mixed together in certain ratios (v/v). The highest antibacterial potential was exhibited by the pure extract of C. citratus, which was even higher than that of the standard drug (ciprofloxacin). The plant extracts and their mixtures were more active against S. aureus as compared to E. coli. No toxic hemolytic effects were observed for the investigated extracts indicating their safe medicinal uses for human beings.
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Affiliation(s)
- Shabbir Hussain
- Institute
of Chemistry, Khwaja Fareed University of
Engineering and Information Technology, Rahim Yar Khan 64200, Pakistan
| | - Warda Javed
- Department
of Chemistry, Lahore Garrison University, DHA Phase VI, Lahore 54792, Pakistan
| | - Affifa Tajammal
- Department
of Chemistry, Lahore Garrison University, DHA Phase VI, Lahore 54792, Pakistan
| | - Muhammad Khalid
- Institute
of Chemistry, Khwaja Fareed University of
Engineering and Information Technology, Rahim Yar Khan 64200, Pakistan
- Centre
for Theoretical and Computational Research, Khwaja Fareed University of Engineering and Information Technology, Rahim Yar Khan, 64200, Pakistan
| | - Nasir Rasool
- Department
of Chemistry, Government College University, Faisalabad 38000, Pakistan
| | - Muhammad Riaz
- Department
of Basic and Applied Chemistry, Faculty
of Science and Technology University of Central Punjab, Lahore 54000, Pakistan
| | - Muhammad Shahid
- Department
of Chemistry and Biochemistry, University
of Agriculture, Faisalabad 38040, Pakistan
| | - Iqbal Ahmad
- Department
of Chemistry, Allama Iqbal Open University, 44000 Islamabad, Pakistan
| | - Riaz Muhammad
- Department
of Chemistry, Lahore Garrison University, DHA Phase VI, Lahore 54792, Pakistan
| | - Syed Adnan Ali Shah
- Faculty
of Pharmacy, Universiti Teknologi MARA Cawangan
Selangor Kampus Puncak Alam, Bandar Puncak Alam 42300, Selangor D. E., Malaysia
- Atta-ur-Rahman
Institute for Natural Product Discovery (AuRIns), Universiti Teknologi MARA Cawangan Selangor Kampus Puncak Alam, Bandar Puncak Alam 42300, Selangor D. E., Malaysia
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16
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Cheesman MJ, Shivashekaregowda NKH, Cock IE. Bacterial Foodborne Illness in Malaysia: Terminalia spp. as a Potential Resource for Treating Infections and Countering Antibiotic Resistance. Malays J Med Sci 2023; 30:42-54. [PMID: 37102050 PMCID: PMC10125245 DOI: 10.21315/mjms2023.30.2.4] [Citation(s) in RCA: 1] [Impact Index Per Article: 0.5] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 09/16/2021] [Accepted: 12/17/2021] [Indexed: 04/28/2023] Open
Abstract
Acute diarrhoea is becoming a major public health problem in Malaysia, with more than 13.5 million cases reported annually. Foodborne bacterial pathogens are a predominant cause of diarrhoea, with infections causing prolonged illness durations and higher patient mortality rates, placing a tremendous burden on the Malaysian economy. Due to increasing incidences of diarrhoea in Malaysia caused by foodborne pathogens and the increasing levels of resistance towards antibiotics from many different classes, new drugs and/or therapies are urgently required. The evidence for plants as new sources of antibiotics has increased dramatically in recent years and there has been a substantial increase in interest in traditional and herbal medicines. Several Terminalia spp. are native to Malaysia, with previous research demonstrating that Terminalia spp. are rich in therapeutic phytochemicals and possess antibacterial properties. However, limited research has been conducted on the native Malaysian Terminalia spp. for their potential as new antibacterial therapies. The current review discusses the types of bacteria, including antibiotic-resistant strains, that cause food poisoning in Malaysia, and reports the phytochemical content and antibacterial properties of eight of these useful plant species. Future directions pertaining to drug discovery pathways are also suggested.
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Affiliation(s)
- Matthew James Cheesman
- School of Pharmacy and Medical Sciences, Gold Coast Campus, Griffith University, Queensland, Australia
| | | | - Ian Edwin Cock
- School of Environment and Science, Nathan Campus, Griffith University, Australia
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17
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Comparative Analysis of Traditional Oriental Herbal Fruits as Potential Sources of Polyphenols and Minerals for Nutritional Supplements. Molecules 2023; 28:molecules28062682. [PMID: 36985653 PMCID: PMC10058731 DOI: 10.3390/molecules28062682] [Citation(s) in RCA: 2] [Impact Index Per Article: 1.0] [Reference Citation Analysis] [Abstract] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 01/28/2023] [Revised: 03/10/2023] [Accepted: 03/13/2023] [Indexed: 03/18/2023] Open
Abstract
There are a plethora of plant species in India, which have been widely used in vegetable dishes, soups, desserts and herbal medicine. In addition to these traditional uses, today there is the extra possibility of also being able to use these plants in the nutritional supplements industry due to their favorable antioxidant and mineral composition. In this sense, thirteen vegetable species—Chanania lanzan, Ziziphus mauritiana, Nilumbo nucifera, Terminalia catappa, Terminalia arjuna, Terminalia bellirica, Terminalia chebula, Lagenaria siceraria, Luffa aegyptiaca, Praecitrullus fistulosus, Benincasa hispida, Citrullus lanatus var. lanatus and Cucurbita maxima—have been analyzed. In this paper we discuss the distribution of polyphenols and minerals (Na, K, Mg, Ca, Al, P, S, Cr, Mn, Fe, Cu, Zn, Mo, As and Pb) in different seed parts (the rhizome, pericarp, carpel, seed coat and kernel) of the above species and their possible use in the nutritional supplements industry. The concentrations of total polyphenols, flavonoids and minerals ranged from 407 to 3144 mg rutin hydrate/100 g, 24 to 3070 mg quercetin/100 g and 1433 to 7928 mg/100 g, respectively. K, Ca, P and S were abundant in these herbal fruits. In two species of herbal fruits, Terminalia arjuna and Terminalia chebula, only part of the seed structure was suitable for use in nutritional supplements.
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18
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Maawadh AM, Al Ahdal K, Al Deeb L, Alshamrani AS, Almohareb T, Alrahlah A. Effectiveness of final cavity disinfectant Terminalia chebula, Malachite, and Indocyanine green, against E. faecalis and on the bond interface of fiber post to radicular dentin. Photodiagnosis Photodyn Ther 2023; 42:103538. [PMID: 37001714 DOI: 10.1016/j.pdpdt.2023.103538] [Citation(s) in RCA: 6] [Impact Index Per Article: 3.0] [Reference Citation Analysis] [Abstract] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 03/04/2023] [Revised: 03/13/2023] [Accepted: 03/24/2023] [Indexed: 03/31/2023]
Abstract
AIMS Evaluation of antimicrobial efficacy of contemporary disinfection Malachite green (MG), Terminalia chebula (T. chebula), and Indocyanine green (ICG)) against E. faecalis and their effect on push-out bond strength (PBS) of glass fiber post (GFP) bonded to root dentin in comparison to conventional irrigation used (Sodium hypochlorite (NaOCl) + Ethylene diamine tetraacetic acid (17% EDTA). MATERIALS AND METHODS Root canal treatment was initiated on sixty human single-rooted premolars and working length was established. The canals were enlarged using ProTaper universal system till F3. The prepared canals were obturated with Gutta-percha (GP) and AH Plus sealer followed by post-space preparation. Twenty canals contained a standard strain of E. faecalis (n=5 in each group) cultured overnight at 37 °C in BHI broth. All the samples were randomly allocated into four groups based on post-space irrigation. (n = 10) Group 1: 5.25% NaOCl+17% EDTA (Control), Group 2: 5.25% NaOCl + ICG, Group 3: 5.25% NaOCl + MG and Group 4: 5.25% NaOCl + T. chebula. Survival rates of E. faecalis were evaluated on the twenty specimens. GFP was placed on the remaining samples using self-etch dual-cure pastes and sectioning was performed in 1 mm thick slices. PBS and failure mode were analyzed using a universal testing machine and stereomicroscope. One-way analysis of variance (ANOVA) and Tukey multiple comparison t-tests were used for data analysis. (p = 0.05). RESULTS 5.25% NaOCl+17% EDTA demonstrated (0.11±0.01 CFU/mL) the lowest survival rate of E. faecalis. However, Group 2 (5.25% NaOCl + ICG) exhibited the highest survival rate (0.52 ± 0.10). Furthermore, the coronal third of group 4 (5.25% NaOCl + T. chebula) (8.71±0.25 MPa) specimens demonstrated the highest PBS of GFP. However, an apical section of group 2 samples (5.25% NaOCl + ICG) (1.21±0.71 MPa) displayed the lowest outcome of bond integrity. CONCLUSION Terminalia chebula when used as a final canal disinfectant seems to be promising in improving canal sterility and the bond strength of GFP to the root dentin.
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Affiliation(s)
- Ahmed M Maawadh
- Department of Restorative Dental Sciences, College of Dentistry, King Saud University, Riyadh 11545, Saudi Arabia
| | - Khold Al Ahdal
- Department of Restorative Dental Sciences, College of Dentistry, King Saud University, Riyadh 11545, Saudi Arabia
| | - Laila Al Deeb
- Department of Restorative Dental Sciences, College of Dentistry, King Saud University, Riyadh 11545, Saudi Arabia
| | - Ahoud S Alshamrani
- Department of Restorative Dental Sciences, College of Dentistry, King Saud University, Riyadh 11545, Saudi Arabia
| | - Thamer Almohareb
- Department of Restorative Dental Sciences, College of Dentistry, King Saud University, Riyadh 11545, Saudi Arabia
| | - Ali Alrahlah
- Department of Restorative Dental Sciences, College of Dentistry, King Saud University, Riyadh 11545, Saudi Arabia.
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Cock IE, Luwaca N, Van Vuuren SF. The traditional use of Southern African medicinal plants to alleviate fever and their antipyretic activities. JOURNAL OF ETHNOPHARMACOLOGY 2023; 303:115850. [PMID: 36306931 DOI: 10.1016/j.jep.2022.115850] [Citation(s) in RCA: 1] [Impact Index Per Article: 0.5] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Subscribe] [Scholar Register] [Received: 07/29/2022] [Revised: 10/05/2022] [Accepted: 10/16/2022] [Indexed: 06/16/2023]
Abstract
ETHNOPHARMACOLOGICAL RELEVANCE Multiple plant species are used traditionally in southern Africa to treat fever. This review summarises the traditional use, highlights promising plant species and focuses on validation studies to highlight future areas of research. AIM OF THE STUDY This study aimed to critically review the use of southern African plants to alleviate fever. Additionally, an examination of research into those plants was undertaken to highlight gaps in the literature with the aim of stimulating further research in this field. MATERIALS AND METHODS Ethnobotanical books, reviews and primary research studies were searched to identify plant species traditionally used to treat fever in southern Africa and to summarise the current knowledge and identify areas requiring further research. This study was non-biased and did not have any taxonomic preferences, nor did it favour any southern African healing systems over the others. RESULTS The traditional therapeutic uses of 203 southern African plant species to alleviate fever were recorded. Leaves and roots/bulbs/rhizomes were the most commonly used plant part, and they were most commonly prepared as decoctions or infusions and consumed orally. Notably, only twenty-two species (∼11% of the identified species) have been scientifically evaluated for antipyretic properties, with substantially fewer mechanistic studies evident. CONCLUSIONS Despite the availability of ethnobotanical records, the antipyretic properties of southern African medicinal plants are poorly reported. Indeed, the efficacy of most plants is yet to be verified and very few mechanistic studies are available.
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Affiliation(s)
- I E Cock
- Centre for Planetary Health and Food Security, Nathan Campus, Griffith University, 170 Kessels Rd, Nathan, Queensland, 4111, Australia; School of Environment and Science, Nathan Campus, Griffith University, 170 Kessels Rd, Nathan, Queensland, 4111, Australia
| | - N Luwaca
- Department of Pharmacy and Pharmacology, Faculty of Health Sciences, University of the Witwatersrand, Parktown, 2193, South Africa
| | - S F Van Vuuren
- Department of Pharmacy and Pharmacology, Faculty of Health Sciences, University of the Witwatersrand, Parktown, 2193, South Africa.
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Understanding the Seasonal Effect of Metabolite Production in Terminalia catappa L. Leaves through a Concatenated MS- and NMR-Based Metabolomics Approach. Metabolites 2023; 13:metabo13030349. [PMID: 36984789 PMCID: PMC10053923 DOI: 10.3390/metabo13030349] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 12/22/2022] [Revised: 02/17/2023] [Accepted: 02/23/2023] [Indexed: 03/03/2023] Open
Abstract
Terminalia catappa L. (Combretaceae) is a medicinal plant that is part of the Brazilian biodiversity; this plant is popularly used for the treatment of a wide range of diseases. To better understand the chemical composition of T. catappa in different seasons, we conducted a thorough study using LC-MS and NMR data analysis techniques. The study helped obtain a chemical profile of the plant ethanolic extracts in different seasons of the year (spring, summer, autumn, and winter). The dereplication of LC-HRMS data allowed the annotation of 90 compounds in the extracts of T. catappa (hydrolyzable tannins, ellagic acid derivatives, and glycosylated flavonoids). Triterpenes and C-glycosyl flavones were the compounds that significantly contributed to differences observed between T. catappa plant samples harvested in autumn/winter and spring, respectively. The variations observed in the compound composition of the plant leaves may be related to processes induced by environmental stress and leaf development. Data fusion applied in the metabolomic profiling study allowed us to identify metabolites with greater confidence, and provided a better understanding regarding the production of specialized metabolites in T. catappa leaves under different environmental conditions, which may be useful to establish appropriate quality criteria for the standardization of this medicinal plant.
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Yakubu Y, Ahmad MT, Chong CM, Ismail IS, Shaari K. Phenolic content of Terminalia catappa L. leaf and toxicity evaluation on red hybrid tilapia (Oreochromis sp.). JOURNAL OF FISH BIOLOGY 2023; 102:358-372. [PMID: 36333916 DOI: 10.1111/jfb.15266] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Subscribe] [Scholar Register] [Received: 08/12/2022] [Accepted: 10/28/2022] [Indexed: 06/16/2023]
Abstract
Despite the use of Terminalia catappa (TC) leaf by traditional fish farmers around the world to improve the health status of cultured fish, there is a paucity of information on comprehensive metabolite profile and the maximum safe dose of the plant. This study aims at profiling the methanol leaf extract of T. catappa, quantifying total phenolic content (TPC) as well as the total flavonoid content (TFC) and evaluating its acute toxicity on blood, plasma biochemical parameters and histopathology of some vital organs in red hybrid tilapia (Oreochromis sp.). The experimental fish were acclimatised for 2 weeks and divided into six groups. Group (1) served as a control group and was administered 0.2 ml,g-1 of phosphate buffer saline (PBS). Groups 2-6 were orally administered T. catappa leaf extracts (0.2 ml.50 g-1 ) in the following sequence; 31.25, 62.5, 125, 250 and 500 mg.kg-1 body weight. The metabolites identified in T. catappa using liquid chromatography-tandem mass electrospray ionisation spectrometry (LC-ESI-MS/MS) revealed the presence of organic acids, hydrolysable tannins, phenolic acids and flavonoids. Phenolic quantification revealed reasonable quantity of phenolic compounds (217.48 μg GAEmg-1 for TPC and 91.90 μg. QCEmg-1 for TFC). Furthermore, there was no significant difference in all the tested doses in terms of blood parameters and plasma biochemical analysis except for the packed cell volume (PCV) at 500 mg.kg-1 when compared to the control. Significant histopathological changes were observed in groups administered with the extract at 125, 250 and 500 mg.kg-1 doses. To a very large extent it is therefore safe to administer the extract at 31.25 and 62.5 mg.kg-1 in tilapia.
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Affiliation(s)
- Yahaya Yakubu
- Department of Chemistry, Faculty of Science, Universiti Putra Malaysia, Serdang, Selangor, Malaysia
- Department of Pure and Applied Chemistry, Faculty of Science, Kaduna State University, Kaduna, Nigeria
| | - Muhammad T Ahmad
- Veterinary Teaching Hospital, Ahmadu Bello University, Zaria, Nigeria
- Faculty of Veterinary Medicine, Ahmadu Bello University Zaria, Zaria, Nigeria
| | - Chou M Chong
- Laboratory of Marine Biotechnology, Institute of Bioscience, Universiti Putra Malaysia, Serdang, Malaysia
| | - Intan S Ismail
- Laboratory of Natural Products, Institute of Bioscience, Universiti Putra Malaysia, Serdang, Malaysia
| | - Khozirah Shaari
- Laboratory of Natural Products, Institute of Bioscience, Universiti Putra Malaysia, Serdang, Malaysia
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Isolation and Bioassay of a New Terminalone A from Terminalia arjuna. Molecules 2023; 28:molecules28031015. [PMID: 36770680 PMCID: PMC9919985 DOI: 10.3390/molecules28031015] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 12/21/2022] [Revised: 01/06/2023] [Accepted: 01/10/2023] [Indexed: 01/20/2023] Open
Abstract
Terminalia arjuna possesses significant cardioprotective, antidiabetic and antioxidant properties as these properties are described in Ayurveda. In the present study, three flavonoids were isolated through the separation and chromatographic purification of the whole plant material of T. arjuna. Spectroscopic characterization identified one of them as a new flavonoid "Terminalone A (1)" and two known flavonoids i.e., 6-hydroxy-2-(4-hydroxyphenyl)-7-methoxy-4H-chromen-4-one (2) and 2-(3,4-dihydroxyphenyl)-5,7-dihydroxy-4H-chromen-4-one (3). The bioactivity studies showed considerable antibacterial and antioxidant (DPPH radical scavenging) potential for all the three compounds 1-3 where the compound 1 showed strong antibacterial and antioxidant activity.
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Antiplasmodial Properties of Aqueous and Ethanolic Extracts of Ten Herbal Traditional Recipes Used in Thailand against Plasmodium falciparum. Trop Med Infect Dis 2022; 7:tropicalmed7120417. [PMID: 36548672 PMCID: PMC9786625 DOI: 10.3390/tropicalmed7120417] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 10/18/2022] [Revised: 11/28/2022] [Accepted: 11/30/2022] [Indexed: 12/11/2022] Open
Abstract
This study evaluated the in vitro and in vivo antiplasmodial efficacy and toxicity of aqueous and ethanolic extracts from traditional recipes used in Thailand. The aqueous and ethanolic extracts of ten traditional recipes were tested for in vitro antiplasmodial activity (parasite lactate dehydrogenase assay), cytotoxicity (MTT assay), and hemolysis). Oxidant levels were measured using cell-permeable probe 5-(and-6)-chloromethyl-2',7'-dichlorodihydrofluorescein diacetate fluorescent dye-based assays. The best candidate was chosen for testing in mouse models using 4-day suppressive and acute toxicity assays. An in vitro study showed that ethanolic extracts and three aqueous extracts exhibited antiplasmodial activity, with an IC50 in the range of 2.8-15.5 µg/mL. All extracts showed high CC50 values, except for ethanolic extracts from Benjakul, Benjalotiga, and Trikatuk in HepG2 and Benjalotiga and aqueous extract from Chan-tang-ha in a Vero cell. Based on the results of the in vitro antiplasmodial activity, an aqueous extract of Triphala was chosen for testing in mouse models. The aqueous extract of Triphala exhibited good antiplasmodial activity, was safe at an oral dose of 2 g/kg, and is a potential candidate as a new source for the development of antimalarial drugs.
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Ou-Yang JR, Wang QF, Li MM, Yue HL, He HP. Chemical constituents isolated from the fruits of Terminalia chebula Retz and their α-glucosidase inhibitory activities. BIOCHEM SYST ECOL 2022. [DOI: 10.1016/j.bse.2022.104424] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 11/29/2022]
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Santos Silva J, França Ferreira ÉL, Maciel Lima A, de Farias RRS, Quirino Araújo B, Quilles Junior JC, Lima Santos RR, de Amorim Carvalho FA, Rai M, Vieira Júnior GM, Chaves MH. Four new cycloartane-type triterpenoids from the leaves of Combretum mellifluum Eichler: assessment of their antioxidant and antileishmanial activities. JOURNAL OF TOXICOLOGY AND ENVIRONMENTAL HEALTH. PART A 2022; 85:364-375. [PMID: 34933666 DOI: 10.1080/15287394.2021.2015030] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Subscribe] [Scholar Register] [Indexed: 06/14/2023]
Abstract
The beneficial pharmacological actions including antioxidant effects as an antileishmanial, antibacterial, antifungal, antidiabetic, anti-inflammatory, antitumor, antiviral, and analgesic of compounds isolated from Combretum mellifluum Eichler (Combretaceae) are well established. The aim of the present study was to determine the phytochemistry as well as assess the antioxidant and antileishmanial activities of the leaves from Combretum mellifluum Eichler (Combretaceae). Analysis of ethanolic extract resulted in isolation and identification of two epimeric mixtures of four previously unknown cycloartane-type triterpenoids, methyl quadrangularate M and methyl 24-epiquadrangularate M, and 2α,3β,24β-trihydroxy-cycloart-25-ene and 2α, 3β, 24α-trihydroxy-cycloart-25-ene, and eight known compounds. Their structures were using one-dimensional nuclear magnetic resonance (1D NMR), 2D NMR and high-resolution electrospray ionization mass spectroscopy (HRESIMS) analysis. Further, the extract and fractions were tested for antioxidant potential. The ethyl acetate and aqueous fractions demonstrated the highest antioxidant activity against 2,2-dipheny-1-picrylhydrazl (DPPH) free radicals, which correlated directly with total flavonoid content. All extracts and fractions from C. mellifluum Eichler were assessed for antileishmanial activity. The supernatant fraction exhibited highest potential, inhibiting the growth of Leishmania amazonensis with IC50 value 31.29 μg/ml. Our findings provide information on the chemical composition of C. mellifluum and the potential beneficial therapeutic usefulness as an antioxidant agent in various diseases.
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Affiliation(s)
- Jaelson Santos Silva
- Department of Chemistry, Center for Natural Sciences, Universidade Federal do Piauí, Teresina, Brazil
| | - Éverton Leandro França Ferreira
- Department of Chemistry, Center for Natural Sciences, Universidade Federal do Piauí, Teresina, Brazil
- Universidade Federal do Vale do São Francisco, Campus Serra da Capivara, São Raimundo Nonato, Brazil
| | - Amanda Maciel Lima
- Department of Chemistry, Center for Natural Sciences, Universidade Federal do Piauí, Teresina, Brazil
| | | | - Bruno Quirino Araújo
- Department of Chemistry, Center for Natural Sciences, Universidade Federal do Piauí, Teresina, Brazil
| | - José Carlos Quilles Junior
- Department of Cell and Molecular Biology, Ribeirão Preto Medical School (FMRP), Universidade de São Paulo (USP), Brazil
| | - Rodolfo Ritchelle Lima Santos
- Department of Biochemistry and Pharmacology, Medicinal Plants Research Center, NPPM, Universidade Federal do Piauí, Teresina, Brasil
| | - Fernando Aécio de Amorim Carvalho
- Department of Biochemistry and Pharmacology, Medicinal Plants Research Center, NPPM, Universidade Federal do Piauí, Teresina, Brasil
| | - Mahendra Rai
- Department of Biotechnology, Sant Gadge Baba Amravati University, Amravati, India
- Department of Microbiology, Nicolaus Copernicus University, Torun, Poland
| | | | - Mariana Helena Chaves
- Department of Chemistry, Center for Natural Sciences, Universidade Federal do Piauí, Teresina, Brazil
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Shahzad MN, Ahmad S, Tousif MI, Ahmad I, Rao H, Ahmad B, Basit A. Profiling of phytochemicals from aerial parts of Terminalia neotaliala using LC-ESI-MS2 and determination of antioxidant and enzyme inhibition activities. PLoS One 2022; 17:e0266094. [PMID: 35358239 PMCID: PMC8970405 DOI: 10.1371/journal.pone.0266094] [Citation(s) in RCA: 20] [Impact Index Per Article: 6.7] [Reference Citation Analysis] [Abstract] [MESH Headings] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 10/21/2021] [Accepted: 03/14/2022] [Indexed: 12/15/2022] Open
Abstract
OBJECTIVES Owing to extraordinary healing power, Terminalia species have been used in traditional medicine systems to treat various diseases. Many folklore uses of Terminalia neotaliala (Madagascar's almond) included treating arterial hypertension, diabetes, diarrhea, dysentery, colic, oral and digestive candidiasis, intestinal parasitic infections, inflammatory skin conditions, postpartum care, and mycotic infections but nevertheless scientifically explored for its medicinal and pharmacological importance. Therefore, the current study intended to prepare methanolic extract and its fractionation with hexane, chloroform, and butanol followed by evaluation of their polyphenolic content, biological activities, and LCMS analysis. The biological study included antioxidant activity and enzyme inhibition assay i.e., α-glucosidase and urease. The insight study of biologically active secondary metabolites of butanol fraction (BUAE) was performed through LCMS. METHODS The total phenolic content (TPC) and total flavonoid content (TFC) of hydroalcoholic and its fractions were estimated using the Folin-Ciocalteu and aluminum chloride method. The total tannin content (TTC) was determined using the Folin-Denis spectrophotometric method. Similarly, the antioxidant potential of HAAE, HEAE, CFAE, and BUAE was determined using four methods as DPPH (1,1-diphenyl-2-picrylhydrazyl), 2,2-azinobis(3-ethylbenothiazoline)-6-sulfonic acid, cupric reducing antioxidant capacity (CUPRAC), and ferric reducing antioxidant power (FRAP). The sample extracts were also evaluated against two clinically important enzymes i.e., α-glucosidase and urease. RESULTS The BUAE (butanol aerial fraction) showed the highest TPC (234.79 ± 0.12 mg.GAE.g-1 DE), TFC (320.75 ± 12.50 mg.QE.g-1 DE), and TTC (143.36 ± 4.32 mg.TA.Eq.g-1 DE). The BUAE also showed the highest scavenging potential determined by DPPH (642.65 ± 1.11 mg.TEq.g-1 DE) and ABTS (543.17 ± 1.11 mg.TEq.g-1 DE), and the metal-reducing capacity determined by CUPRAC (1510.41 ± 4.45 mg.TEq.g-1 DE) and FRAP (739.81 ± 19.32 mg.TEq.g-1 DE). The LCMS of BUAE identified 18 different biologically active phytoconstituents validating a rich source of hydrolyzable tannins including ellagitannins and gallitannins. CONCLUSION The present study concluded that T. neotaliala is a rich source of polyphenols capable of neutralizing the damage caused by free radical accumulation in the cells and tissues. The significant antioxidant results and identification of high molecular weight hydrolyzable tannins enlightened the medicinal importance of T. neotaliala.
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Affiliation(s)
- Muhammad Nadeem Shahzad
- Department of Pharmaceutical Chemistry, The Islamia University of Bahawalpur, Bahawalpur, Pakistan
| | - Saeed Ahmad
- Department of Pharmaceutical Chemistry, The Islamia University of Bahawalpur, Bahawalpur, Pakistan
| | | | - Imtiaz Ahmad
- Department of Pharmaceutical Chemistry, The Islamia University of Bahawalpur, Bahawalpur, Pakistan
- Department of Medicinal Chemistry, College of Pharmacy, University of Minnesota, Minneapolis, Minnesota, United States of America
| | - Huma Rao
- Department of Pharmaceutical Chemistry, The Islamia University of Bahawalpur, Bahawalpur, Pakistan
| | - Bilal Ahmad
- Department of Pharmaceutical Chemistry, The Islamia University of Bahawalpur, Bahawalpur, Pakistan
| | - Abdul Basit
- Department of Pharmaceutical Chemistry, The Islamia University of Bahawalpur, Bahawalpur, Pakistan
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Courtney R, Cock IE. Comparison of the antibacterial activity of Australian Terminalia spp. extracts against Klebsiella pneumoniae: a potential treatment for ankylosing spondylitis. Inflammopharmacology 2022; 30:207-223. [PMID: 34989930 DOI: 10.1007/s10787-021-00914-8] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 11/17/2021] [Accepted: 12/16/2021] [Indexed: 11/28/2022]
Abstract
Traditional medicines prepared using Terminalia species have been used globally to treat inflammation and pathogenic infections. Recent studies have demonstrated that multiple Asian and African Terminalia spp. inhibit bacterial triggers of some autoimmune inflammatory diseases, including ankylosing spondylitis. Despite this, the effects of Australian Terminalia spp. on a bacterial trigger of ankylosing spondylitis (K. pneumoniae) remain unexplored. Fifty-five extracts from five Australian Terminalia spp. were investigated for K. pneumoniae growth inhibitory activity. Methanolic, aqueous and ethyl acetate extracts of most species and plant parts inhibited K. pneumoniae growth, with varying potencies. Methanolic leaf extracts were generally the most potent bacterial growth inhibitors, with minimum inhibitory concentration (MIC) values of 66 μg/mL (T. ferdinandiana), 128 μg/mL (T. carpenteriae) and 83 μg/mL (T. petiolares). However, the aqueous leaf extract was the most potent T. grandiflora extract (MIC = 87 μg/mL). All T. catappa extracts displayed low growth inhibitory activity. The Terminalia spp. methanolic leaf extracts were examined by liquid chromatography-mass spectrometry (LC-MS) and gas chromatography-mass spectrometry (GC-MS). All contained a relative abundance of simple gallotannins (particularly gallic and chebulic acids), the flavonoid luteolin, as well as the monoterpenoids cineole and terpineol. Notably, all Terminalia spp. were non-toxic or of low toxicity in ALA and HDF toxicity assays, highlighting their potential for preventing the onset of ankylosing spondylitis and treating its symptoms once the disease is established, although this needs to be verified in in vivo systems.
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Affiliation(s)
- Reece Courtney
- Centre for Planetary Health and Food Security, Nathan Campus, Griffith University, 170 Kessels Rd, Nathan, QLD, 4111, Australia.,School of Environment and Science, Nathan Campus, Griffith University, 170 Kessels Rd, Nathan, QLD, 4111, Australia
| | - Ian Edwin Cock
- Centre for Planetary Health and Food Security, Nathan Campus, Griffith University, 170 Kessels Rd, Nathan, QLD, 4111, Australia. .,School of Environment and Science, Nathan Campus, Griffith University, 170 Kessels Rd, Nathan, QLD, 4111, Australia.
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Dlamini BS, Hernandez CE, Chen CR, Shih WL, Hsu JL, Chang CI. In vitro antioxidant, antiglycation, and enzymatic inhibitory activity against α-glucosidase, α-amylase, lipase and HMG-CoA reductase of Terminalia boivinii Tul. BIOCATALYSIS AND AGRICULTURAL BIOTECHNOLOGY 2022. [DOI: 10.1016/j.bcab.2021.102235] [Citation(s) in RCA: 1] [Impact Index Per Article: 0.3] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 12/15/2022]
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Siraj MA, Islam Howlader MS, Islam MA, Irin T, Simal-Gandara J. Regulation of the redox signaling and inflammation by Terminalia myriocarpa leaves and the predictive interactions of it's major metabolites with iNOS and NF-ĸB. JOURNAL OF ETHNOPHARMACOLOGY 2021; 280:114459. [PMID: 34343646 DOI: 10.1016/j.jep.2021.114459] [Citation(s) in RCA: 2] [Impact Index Per Article: 0.5] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Subscribe] [Scholar Register] [Received: 01/24/2021] [Revised: 07/06/2021] [Accepted: 07/24/2021] [Indexed: 06/13/2023]
Abstract
ETHNOPHARMACOLOGICAL RELEVANCE The present study was designed to investigate the regulation of the redox signaling and inflammation by ethanolic leaf extract of Terminalia myriocarpaVan Heurck & Müller (ETM), inspired by the reported antioxidant potential of the plant bark and the anti-edema effect of the same genus. MATERIALS AND METHODS HPLC-DAD dereplication study was conducted to detect the major polyphenolic secondary metabolites. In-vitro DPPH free radical scavenging assay, nitric oxide (NO) scavenging assay, Fe2+ ion chelating ability assay and reducing power assay were conducted to evaluate the antioxidant capacity. The molecular mechanism of anti-inflammation was investigated via assessing the NO and NF-ĸB inhibiting properties in different cell lines. In-vivo carrageenan and histamine-induced edema tests were conducted using established animal models. Pro-inflammatory proteins iNOS and NF-κB were docked against the major metabolites of ETM in the in-silico study. RESULTS HPLC dereplication analysis revealed the presence of considerable amount of ellagic acid, where methyl-(S)-flavogallonate was previously reported in T. myriocarpa. Significant antioxidant activity was found in every in- vitro redox assay conducted. NO was reduced in RAW 264.7 cells, showing 83.67 ± 4.18% inhibitory activity at the highest tested concentration. TNF-α induced NF-κB was also observed to be reduced in 293/NF-кB-luc cells with an inhibitory activity of 66.23 ± 0.81% at the highest dose tested. In-vivo carrageenan-induced edema test demonstrated significant anti-inflammatory activity (p < 0.05; p < 0.01) at both doses of 250 and 500 mg/kg with 60.10% highest reduction in rat paw volume. Using same doses, histamine-induced edema test exhibited mentionable anti-inflammatory potential (p < 0.05; p < 0.01) with 67.91% highest reduction in rat paw volume. Moreover, ellagic acid and methyl-(S)-flavogallonate showed significant binding affinity with iNOS (-8.5 and -8.7 Kcal/moL, respectively) and NF-κB (-7.3 and -7.3 Kcal/moL, respectively). CONCLUSION Mentionable basis was found on behalf of the anti-inflammatory and antioxidant potentials of ETM which might be correlated with its NF-ĸB inhibiting properties.
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Affiliation(s)
- Md Afjalus Siraj
- Department of Pharmaceutical Sciences, Daniel K. Inouye College of Pharmacy, University of Hawaii at Hilo, Hilo, 96720, HI, USA
| | - Md Sariful Islam Howlader
- Department of Pharmaceutical Sciences, Jerry H. Hodge School of Pharmacy, Texas Tech University Health Sciences Center, Amarillo, TX, 79106, USA; Department of Chemistry, Tennessee Technological University, Cookeville, TN, 38501, USA
| | - Md Arman Islam
- Pharmacy Discipline, Life Science School, Khulna University, Khulna, 9208, Bangladesh
| | - Tanzira Irin
- Pharmacy Discipline, Life Science School, Khulna University, Khulna, 9208, Bangladesh; Department of Pharmacy, ASA University Bangladesh, Dhaka, 1207, Bangladesh
| | - Jesus Simal-Gandara
- Nutrition and Bromatology Group, Department of Analytical and Food Chemistry, Faculty of Food Science and Technology, University of Vigo, Ourense Campus, E32004, Ourense, Spain.
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Proximate composition, functional and antimicrobial properties of wild harvest Terminalia carpentariae fruit. JOURNAL OF FOOD MEASUREMENT AND CHARACTERIZATION 2021. [DOI: 10.1007/s11694-021-01182-4] [Citation(s) in RCA: 3] [Impact Index Per Article: 0.8] [Reference Citation Analysis] [Track Full Text] [Subscribe] [Scholar Register] [Indexed: 01/03/2023]
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Haque E, Kamal MS, Tahsin MR, Ahmed R, Choudhury JA, Choudhury AA, Kabir S, Amran MS, Uddin MS. Current Knowledge Regarding Pharmacological Profile and Chemical Constituents of Gynura procumbens. Curr Top Med Chem 2021; 21:2671-2686. [PMID: 34607545 DOI: 10.2174/1568026621666211004094902] [Citation(s) in RCA: 1] [Impact Index Per Article: 0.3] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 03/27/2021] [Revised: 08/19/2021] [Accepted: 08/23/2021] [Indexed: 11/22/2022]
Abstract
Gynura procumbens (Lour.) Merr. is a well-known plant used in the folkloric medicine in tropical Asian countries. The plant is prevalently employed by traditional healers in the treatment of diabetes, cancer, hypertension, inflammation, fever and skin disorders. Several scientific studies reported that, Gynura procumbens possesses considerable therapeutic value for the development of emerging treatment options. The diverse pharmacological effects of this plant are attributed to its vast phytoconstituent content. Different chemical classes including alkaloids, flavonoids, phenolics, steroids, proteins and polysaccharides have been isolated from this plant. In this review, we tried to explore the different aspects of Gynura procumbens as an established medicinal plant. The data gathered here give an indication that the plant Gynura procumbens is a good natural source of chemical compounds with different types of pharmacological actions and these chemical compounds can be used as model for the development of de novo therapeutic agents.
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Affiliation(s)
- Ehfazul Haque
- Molecular Pharmacology and Herbal Drug Research Laboratory, Department of Pharmaceutical chemistry, Faculty of Pharmacy, University of Dhaka, Dhaka 1000. Bangladesh
| | - Mir Shahriar Kamal
- Deaprtment of Pharmacy, Faculty of Pharmacy, University of Dhaka, Dhaka 1000. Bangladesh
| | - Md Rafat Tahsin
- Department of Pharmaceutical Sciences, North South University, Dhaka. Bangladesh
| | - Raju Ahmed
- Molecular Pharmacology and Herbal Drug Research Laboratory, Department of Pharmaceutical chemistry, Faculty of Pharmacy, University of Dhaka, Dhaka 1000. Bangladesh
| | - Jakir Ahmed Choudhury
- Department of Pharmaceutical Technology, Faculty of Pharmacy, University of Dhaka, Dhaka 1000. Bangladesh
| | - Abu Asad Choudhury
- Molecular Pharmacology and Herbal Drug Research Laboratory, Department of Pharmaceutical chemistry, Faculty of Pharmacy, University of Dhaka, Dhaka 1000. Bangladesh
| | - Shaila Kabir
- Molecular Pharmacology and Herbal Drug Research Laboratory, Department of Pharmaceutical chemistry, Faculty of Pharmacy, University of Dhaka, Dhaka 1000. Bangladesh
| | - Md Shah Amran
- Molecular Pharmacology and Herbal Drug Research Laboratory, Department of Pharmaceutical chemistry, Faculty of Pharmacy, University of Dhaka, Dhaka 1000. Bangladesh
| | - Md Sahab Uddin
- Department of Pharmacy, Southeast University, Dhaka. Bangladesh
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Aliyu-Amoo H, Isa HI, Njoya EM, McGaw LJ. Antiproliferative effect of extracts and fractions of the root of Terminalia avicennioides (Combretaceae) Guill and Perr. on HepG2 and Vero cell lines. CLINICAL PHYTOSCIENCE 2021. [DOI: 10.1186/s40816-021-00307-y] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Track Full Text] [Journal Information] [Submit a Manuscript] [Subscribe] [Scholar Register] [Indexed: 11/10/2022] Open
Abstract
Abstract
Background
Terminalia avicennioides Guill and Perr (Combretaceae) is an important West African medicinal plant. The plant is used locally against microbes and parasites in both humans and animals and studies have demonstrated its cytotoxicity potential. Thus, this study was carried out to test the cytotoxic effect of the extracts and fractions of the root of the medicinal plant Terminalia avicennioides Guill and Perr (Combretaceae) in two different cell lines.
Methods
Methanol, ethanol, 30 % ethanol, hot water and cold water extracts and ethylacetate, hexane, chloroform, butanol and residual water fractions, were evaluated at 1000, 750, 500, 250, 100 and 50 µg/mL concentrations, with doxorubicin as positive control. The cells were incubated with the extracts for 48 h at 37 °C in a 5 % CO2 humidified incubator. The inhibition of cell viability, determined with the methyl blue thiazole tetrazolium bromide (MTT) assay, was used to assess the anti-proliferative effect of the extracts, in normal Vero Monkey kidney and human liver cancer (HepG2) cell lines.
Results
There was a concentration-dependent inhibition of cell viability in both the HepG2 and Vero cell lines. For HepG2 cells, antiproliferative effect was highest for the hexane fraction (viability ranged from 19.63 ± 1.10 % to 70.30 ± 1.78 % for 1000 and 50 µg/mL, respectively. For Vero cells, the highest antiproliferative effect, at 1000 µg/mL, was with hexane fraction (cell viability 21.37 ± 3.50 %), while at 50 µg/mL the chloroform fraction demonstrated the highest effect (viability of 86.10 ± 1.95 %).
Conclusions
The extracts and fractions from the root of Terminalia avicennioides have antiproliferative effect on the Vero and HepG2 cell lines tested. However, the extracts and fractions were not more toxic to the HepG2 than to the Vero cells. The cytotoxic effect of stem-bark and leaf extracts could be evaluated in the future to determine its anticancer potential.
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Baldé AM, Balde AO, Bah B, Barry H, Traore S, Bah F, Balde MA, Camara A, Traore MS, Balde ES, Sylla IK, Diallo S. Malaria in Guinean Rural Areas: Prevalence, Management, and Ethnotherapeutic Investigations in Dionfo, Sub-Prefecture of Labe. PLANTA MEDICA 2021; 87:850-859. [PMID: 34229354 DOI: 10.1055/a-1519-5847] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [MESH Headings] [Track Full Text] [Subscribe] [Scholar Register] [Indexed: 06/13/2023]
Abstract
As part of a validation program of antimalarial traditional recipes, an ethnotherapeutic approach was applied in Dionfo, a meso-endemic Guinean rural area where conventional health facilities are insufficient. A prevalence investigation indicated a malarial burden of 4.26%. Ethnomedical and ethnobotanical surveys led to a collection of 63 plant species used against malaria from which Terminalia albida (Combretaceae) was one of the most cited. Ethnotherapeutic evaluation of a remedy based on T. albida was applied to 9 voluntary patients suffering from uncomplicated malaria. Treatment of 7 to 14 days led to an improvement of clinical symptoms and a complete parasite clearance achievement of 8/9 patients without side effects. In addition to antiplasmodial activity in vitro and in vivo previously described, this study indicates an efficacy to support the antimalarial traditional use of T. albida, which could constitute a first-aid treatment when access to other medicines is delayed in the Dionfo community. Ethnotherapeutical investigation could be a valuable approach to guide subsequent investigations on traditional remedies.
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Affiliation(s)
- Aliou Mamadou Baldé
- Institut de Recherche et de Développement des Plantes Médicinales et Alimentaires de Guinée, Dubréka, BP 6411 Conakry, Guinée
- Faculté des Sciences et Techniques de la Santé, Université Gamal Abdel Nasser de Conakry, Guinée
| | - Alpha Oumar Balde
- Faculté des Sciences et Techniques de la Santé, Université Gamal Abdel Nasser de Conakry, Guinée
| | - Bilo Bah
- Faculté des Sciences et Techniques de la Santé, Université Gamal Abdel Nasser de Conakry, Guinée
| | - Halimatou Barry
- Faculté des Sciences et Techniques de la Santé, Université Gamal Abdel Nasser de Conakry, Guinée
| | - Saidou Traore
- Institut de Recherche et de Développement des Plantes Médicinales et Alimentaires de Guinée, Dubréka, BP 6411 Conakry, Guinée
| | - Fatoumata Bah
- Faculté des Sciences, Université Julius Nyéréré de Kankan
| | - Mamadou Aliou Balde
- Faculté des Sciences et Techniques de la Santé, Université Gamal Abdel Nasser de Conakry, Guinée
| | - Aissata Camara
- Faculté des Sciences et Techniques de la Santé, Université Gamal Abdel Nasser de Conakry, Guinée
| | - Mohamed Sahar Traore
- Institut de Recherche et de Développement des Plantes Médicinales et Alimentaires de Guinée, Dubréka, BP 6411 Conakry, Guinée
- Faculté des Sciences et Techniques de la Santé, Université Gamal Abdel Nasser de Conakry, Guinée
| | - Elhadj Saidou Balde
- Institut de Recherche et de Développement des Plantes Médicinales et Alimentaires de Guinée, Dubréka, BP 6411 Conakry, Guinée
- Faculté des Sciences et Techniques de la Santé, Université Gamal Abdel Nasser de Conakry, Guinée
| | - Ibrahima Kalil Sylla
- Institut de Recherche et de Développement des Plantes Médicinales et Alimentaires de Guinée, Dubréka, BP 6411 Conakry, Guinée
| | - Sadou Diallo
- Tradithérapeute professionnel, Dionfo-Centre, Labé, Guinée
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Identification of Human Kinin-Forming Enzyme Inhibitors from Medicinal Herbs. Molecules 2021; 26:molecules26144126. [PMID: 34299400 PMCID: PMC8307503 DOI: 10.3390/molecules26144126] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 06/02/2021] [Revised: 06/26/2021] [Accepted: 07/02/2021] [Indexed: 11/18/2022] Open
Abstract
The goal of this study was to assess the pharmacological effects of black tea (Camellia sinensis var. assamica) water extract on human kinin-forming enzymes in vitro. Tea is a highly consumed beverage in the world. Factor XII (FXII, Hageman factor)-independent- and -dependent activation of prekallikrein to kallikrein leads to the liberation of bradykinin (BK) from high-molecular-weight kininogen (HK). The excessive BK production causes vascular endothelial and nonvascular smooth muscle cell permeability, leading to angioedema. The prevalence of angiotensin-converting enzyme inhibitor (ACEI)-induced angioedema appears to be through BK. Both histamine and BK are potent inflammatory mediators. However, the treatments for histamine-mediated angioedema are unsuitable for BK-mediated angioedema. We hypothesized that long-term consumption of tea would reduce bradykinin-dependent processes within the systemic and pulmonary vasculature, independent of the anti-inflammatory actions of polyphenols. A purified fraction of the black tea water extract inhibited both kallikrein and activated FXII. The black tea water extracts inhibited factor XII-induced cell migration and inhibited the production of kallikrein on the endothelial cell line. We compared the inhibitory effects of the black tea water extract and twenty-three well-known anti-inflammatory medicinal herbs, in inhibiting both kallikrein and FXII. Surprisingly, arjunglucoside II specifically inhibited the activated factor XII (FXIIa), but not the kallikrein and the activated factor XI. Taken together, the black tea water extract exerts its anti-inflammatory effects, in part, by inhibiting kallikrein and activated FXII, which are part of the plasma kallikrein–kinin system (KKS), and by decreasing BK production. The inhibition of kallikrein and activated FXII represents a unique polyphenol-independent anti-inflammatory mechanism of action for the black tea.
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Chikowe I, Mtewa AG, Tembo D, Smith D, Ibrahim E, Mwamatope B, Nkhungulu J, Kumpalume P, Maroyi A. Potential of Malawi's medicinal plants in Covid-19 disease management: A review. Malawi Med J 2021; 33:85-107. [PMID: 34777704 PMCID: PMC8560350 DOI: 10.4314/mmj.v33i2.4] [Citation(s) in RCA: 8] [Impact Index Per Article: 2.0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 12/23/2022] Open
Abstract
The Coronavirus Disease 2019 (COVID-19) caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has triggered an international pandemic that has led to significant public health problems. To date, limited evidence exists to suggest that drugs are effective against the disease. As possible treatments are being investigated, herbal medicines have shown potential for producing novel antiviral agents for the COVID-19 disease. Aim This review explored the potential of Malawi's traditional medicinal plants for the management of COVID-19. Methods The authors searched on PubMed and Google scholar for medicinal plants that are used in Malawi and published in openly available peer reviewed journals. Plants linked with antiviral treatment, anti-COVID-19 activity or COVID-19 symptoms management were targeted. These included activity against pneumonia, inflammation, cough, difficulty in breathing, pain/aches, fever, diarrhoea, rheumatism, fatigue, asthma, immunocompromised and cardiovascular diseases. Results 11 studies were found with 306 plant species. 127 plant species had at least one COVID-19 related pharmacological activity. Of these plant species, the number of herbal entities used for each indication was: pain/aches (87), fever (2), pneumonia (9), breathing/asthma problems (5), coughing (11), diarrhoea (1), immunosuppression (8), blood issues (10), fatigue (2), heart problems (11), inflammation (8), rheumatism (10) and viral diseases (12). Thirty (30) species were used for more than one disease and Azedarachta indica topped the list (6 of the 13 COVID-19 related diseases). The majority of the species had phytochemicals known to have antiviral activity or mechanisms of actions linked to COVID-19 and consequent diseases' treatment pathways. Conclusion Medicinal plants are a promising source of compounds that can be used for drug development of COVID-19 related diseases. This review highlights potential targets for the World Health Organization and other research entities to explore in order to assist in controlling the pandemic.
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Affiliation(s)
- Ibrahim Chikowe
- Pharmacy Department, College of Medicine, University of Malawi, Blantyre, Malawi/Kamuzu University of Health Sciences
| | - Andrew G Mtewa
- Chemistry Section, Malawi Institute of Technology, Malawi University of Science and Technology, Thyolo, Malawi
| | - David Tembo
- The Polytechnic, University of Malawi, Blantyre, Malawi/Malawi University of Business and Applied Sciences
| | - Dallas Smith
- Pharmacy Department, College of Medicine, University of Malawi, Blantyre, Malawi/Kamuzu University of Health Sciences
| | - Edna Ibrahim
- The Polytechnic, University of Malawi, Blantyre, Malawi/Malawi University of Business and Applied Sciences
| | | | - Justin Nkhungulu
- Pharmacy Department, College of Medicine, University of Malawi, Blantyre, Malawi/Kamuzu University of Health Sciences
| | - Peter Kumpalume
- Pharmacy Department, College of Medicine, University of Malawi, Blantyre, Malawi/Kamuzu University of Health Sciences
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Baldé MA, Tuenter E, Traoré MS, Peeters L, Matheeussen A, Cos P, Caljon G, Vermeyen T, Herrebout W, Balde AM, Foubert K, Pieters L. Antiplasmodial Oleanane Triterpenoids from Terminalia albida Root Bark. JOURNAL OF NATURAL PRODUCTS 2021; 84:666-675. [PMID: 33667089 DOI: 10.1021/acs.jnatprod.0c01119] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [MESH Headings] [Track Full Text] [Subscribe] [Scholar Register] [Indexed: 06/12/2023]
Abstract
Phytochemical investigation of the n-BuOH extract of the roots of Terminalia albida Sc. Elliot (Combretaceae) led to the isolation and identification of 10 oleanane triterpenoids (1-10), among which six new compounds, i.e., albidanoside A (2), albidic acid A (4), albidinolic acid (5), albidienic acid (8), albidolic acid (9), and albidiolic acid (10), and two triterpenoid aglycones, i.e., albidic acid B (6) and albidic acid C (7), were isolated here for the first time from a natural source, along with two known compounds. The structures of these constituents were established by means of 1D and 2D NMR spectroscopy and ESI mass spectrometry. The isolated compounds were evaluated for their antiplasmodial and antimicrobial activity against the chloroquine-resistant strain Plasmodium falciparum K1, Candida albicans, and Staphylococcus aureus. Compounds 1-4, 6, 7, and 8 showed moderate antiplasmodial activity with IC50 values between 5 and 15 μM. None of the tested compounds were active against C. albicans or S. aureus. These findings emphasize the potential of T. albida as a source for discovery of new antiplasmodial compounds.
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Affiliation(s)
- Mamadou A Baldé
- Natural Products & Food Research and Analysis (NatuRA), Department of Pharmaceutical Sciences, University of Antwerp, Universiteitsplein 1, B-2610 Antwerp, Belgium
| | - Emmy Tuenter
- Natural Products & Food Research and Analysis (NatuRA), Department of Pharmaceutical Sciences, University of Antwerp, Universiteitsplein 1, B-2610 Antwerp, Belgium
| | - Mohamed S Traoré
- Department of Pharmacy, University Gamal Abdel Nasser of Conakry, BP 1017, Conakry, Guinea
- Research and Valorization Center on Medicinal Plants Dubreka, BP 6411, Conakry, Guinea
| | - Laura Peeters
- Natural Products & Food Research and Analysis (NatuRA), Department of Pharmaceutical Sciences, University of Antwerp, Universiteitsplein 1, B-2610 Antwerp, Belgium
| | - An Matheeussen
- Laboratory of Microbiology, Parasitology and Hygiene (LMPH), Faculty of Pharmaceutical, Biomedical and Veterinary Sciences, University of Antwerp, Universiteitsplein 1, B-2610 Antwerp, Belgium
| | - Paul Cos
- Laboratory of Microbiology, Parasitology and Hygiene (LMPH), Faculty of Pharmaceutical, Biomedical and Veterinary Sciences, University of Antwerp, Universiteitsplein 1, B-2610 Antwerp, Belgium
| | - Guy Caljon
- Laboratory of Microbiology, Parasitology and Hygiene (LMPH), Faculty of Pharmaceutical, Biomedical and Veterinary Sciences, University of Antwerp, Universiteitsplein 1, B-2610 Antwerp, Belgium
| | - Tom Vermeyen
- MolSpec Research Group, Department of Chemistry, University of Antwerp, Groenenborgerlaan 171, 2020 Antwerp, Belgium
| | - Wouter Herrebout
- MolSpec Research Group, Department of Chemistry, University of Antwerp, Groenenborgerlaan 171, 2020 Antwerp, Belgium
| | - Aliou M Balde
- Department of Pharmacy, University Gamal Abdel Nasser of Conakry, BP 1017, Conakry, Guinea
- Research and Valorization Center on Medicinal Plants Dubreka, BP 6411, Conakry, Guinea
| | - Kenn Foubert
- Natural Products & Food Research and Analysis (NatuRA), Department of Pharmaceutical Sciences, University of Antwerp, Universiteitsplein 1, B-2610 Antwerp, Belgium
| | - Luc Pieters
- Natural Products & Food Research and Analysis (NatuRA), Department of Pharmaceutical Sciences, University of Antwerp, Universiteitsplein 1, B-2610 Antwerp, Belgium
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Cock IE, Ndlovu N, Van Vuuren SF. The use of South African botanical species for the control of blood sugar. JOURNAL OF ETHNOPHARMACOLOGY 2021; 264:113234. [PMID: 32768640 DOI: 10.1016/j.jep.2020.113234] [Citation(s) in RCA: 7] [Impact Index Per Article: 1.8] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Subscribe] [Scholar Register] [Received: 05/15/2020] [Revised: 07/07/2020] [Accepted: 07/29/2020] [Indexed: 06/11/2023]
Abstract
ETHNOPHARMACOLOGICAL RELEVANCE Diabetes mellitus (DM) is one of the most prevalent diseases globally and is of considerable concern to global health. Approximately 425 million people are estimated to have DM globally and this is predicted to increase to >642 million by 2040. Whilst the prevalence of DM in South Africa is slightly lower than the global average, it is expected to rise rapidly in future years as more South Africans adopt a high calorie "westernised" diet. Traditional medicines offer an alternative for the development of new medicines to treat DM and the usage of South African plants is relatively well documented. AIM OF THE STUDY To critically review the literature on the anti-diabetic properties of South African plants and to document plant species used for the treatment of DM. Thereafter, a thorough examination of the related research will highlight where research is lacking in the field. MATERIALS AND METHODS A review of published ethnobotanical books, reviews and primary scientific studies was undertaken to identify plants used to treat DM in traditional South African healing systems and to identify gaps in the published research. The study was non-biased, without taxonomic preference and included both native and introduced species. To be included, species must be recorded in the pharmacopeia of at least one South African ethnic group for the treatment of DM. RESULTS One hundred and thirty-seven species are recorded as therapies for DM, with leaves and roots most commonly used. The activity of only 43 of these species have been verified by rigorous testing, and relatively few studies have examined the mechanism of action. CONCLUSION Despite relatively extensive ethnobotanical records and a diverse flora, the anti-diabetic properties of South African medicinal plants is relatively poorly explored. The efficacy of most plants used traditionally to treat DM are yet to be verified and few mechanistic studies are available. Further research is required in this field.
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Affiliation(s)
- I E Cock
- School of Environment and Science, Nathan Campus, Griffith University, 170 Kessels Rd, Nathan, Queensland, 4111, Australia; Environmental Futures Research Institute, Nathan Campus, Griffith University, 170 Kessels Rd, Nathan, Queensland, 4111, Australia
| | - N Ndlovu
- Department of Pharmacy and Pharmacology, Faculty of Health Sciences, University of the Witwatersrand, Parktown, Gauteng, 2193, South Africa
| | - S F Van Vuuren
- Department of Pharmacy and Pharmacology, Faculty of Health Sciences, University of the Witwatersrand, Parktown, Gauteng, 2193, South Africa.
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Cock IE, Cheesman M. Plants of the genus Terminalia: Phytochemical and antioxidant profiles, proliferation, and cancer. Cancer 2021. [DOI: 10.1016/b978-0-12-819547-5.00044-4] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 10/22/2022]
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Antioxidant, anti-inflammatory and hepatoprotective activities of Terminalia bellirica and its bioactive component ellagic acid against diclofenac induced oxidative stress and hepatotoxicity. Toxicol Rep 2020; 8:44-52. [PMID: 33391996 PMCID: PMC7772792 DOI: 10.1016/j.toxrep.2020.12.010] [Citation(s) in RCA: 56] [Impact Index Per Article: 11.2] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 01/10/2020] [Revised: 11/07/2020] [Accepted: 12/10/2020] [Indexed: 12/19/2022] Open
Abstract
Long term usage and overdose of diclofenac (DCF), an anti-inflammatory drug is known to cause oxidative stress and liver injury. The present study reports the antioxidant, anti-inflammatory and hepatoprotective activities of Terminalia bellirica (Tb) fruit aqueous and ethyl acetate extracts and its bioactive compound ellagic acid (EA) against DCF-induced toxicity. in vitro antioxidant activities were measured by ABTS and FRAP assays while anti‐inflammatory activity was assessed by the albumin denaturation method. The adverse effects of DCF and hepatoprotective potential of Tb extracts and EA were assessed in serum and liver tissue of rats after oral administration for 21 days. Silymarin was used as standard hepatoprptective agent for comparison. Hepatic markers analyzed in serum included ALP, GPT, GOT, LDH, γ-glutamyl transferase, total protein, creatinine, and uric acid while superoxide dismutase (SOD) and catalase (CAT) were analyzed in liver tissue. The EA exhibited superior ABTS radical scavenging, FRAP, and anti-inflammatory activities as compared to fruit extracts. DCF treatment led to rise in the levels of most of the serum hepatic markers with decline in total serum protein as well as SOD and CAT in liver tissue. The supplementation of extracts, EA and silymarin in DCF treated rats significantly reduced the adverse effects of DCF on serum and tissue markers. Histopathology of the liver indicated that extracts and EA significantly decreased the degree of liver fibrosis. The hepatoprotective ability of EA was comparable to the silymarin but activity of Tb fruit extracts was little lower. Among fruit extracts ethyl acetate extract exhibited better activity than aqueous extract. The results revealed that ellagic acid and T. bellirica fruit extracts have potential to mitigate oxidative stress and hepatotoxicity produced by long term use of diclofenac.
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Cock IE, Van Vuuren SF. The traditional use of southern African medicinal plants for the treatment of bacterial respiratory diseases: A review of the ethnobotany and scientific evaluations. JOURNAL OF ETHNOPHARMACOLOGY 2020; 263:113204. [PMID: 32730881 PMCID: PMC7383173 DOI: 10.1016/j.jep.2020.113204] [Citation(s) in RCA: 7] [Impact Index Per Article: 1.4] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Subscribe] [Scholar Register] [Received: 03/18/2020] [Revised: 07/07/2020] [Accepted: 07/20/2020] [Indexed: 05/04/2023]
Abstract
ETHNOPHARMACOLOGICAL RELEVANCE Multiple plant species were used traditionally in southern Africa to treat bacterial respiratory diseases. This review summarises this usage and highlights plant species that are yet to be verified for these activities. AIM OF THE STUDY This manuscript reviews the traditional usage of southern African plant species to treat bacterial respiratory diseases with the aim of highlighting gaps in the literature and focusing future studies. MATERIALS AND METHODS An extensive review of ethnobotanical books, reviews and primary scientific studies was undertaken to identify southern African plants which are used in traditional southern African medicine to treat bacterial respiratory diseases. We also searched for southern African plants whose inhibitory activity against bacterial respiratory pathogens has been conmfirmed, to highlight gaps in the literature and focus future studies. RESULTS One hundred and eighty-seven southern African plant species are recorded as traditional therapies for bacterial respiratory infections. Scientific evaluations of 178 plant species were recorded, although only 42 of these were selected for screening on the basis of their ethnobotanical uses. Therefore, the potential of 146 species used teraditionally to treat bacterial respiratory diseases are yet to be verified. CONCLUSIONS The inhibitory properties of southern African medicinal plants against bacterial respiratory pathogens is relatively poorly explored and the antibacterial activity of most plant species remains to be verified.
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Affiliation(s)
- Ian E Cock
- School of Environment and Science, Nathan Campus, Griffith University, 170 Kessels Rd, Nathan, Queensland, 4111, Australia; Environmental Futures Research Institute, Nathan Campus, Griffith University, 170 Kessels Rd, Nathan, Queensland, 4111, Australia.
| | - Sandy F Van Vuuren
- Department of Pharmacy and Pharmacology, Faculty of Health Sciences, University of the Witwatersrand, Parktown, 2193, South Africa
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Gupta A, Pandey AK. Aceclofenac-induced hepatotoxicity: An ameliorative effect of Terminalia bellirica fruit and ellagic acid. World J Hepatol 2020; 12:949-964. [PMID: 33312421 PMCID: PMC7701975 DOI: 10.4254/wjh.v12.i11.949] [Citation(s) in RCA: 19] [Impact Index Per Article: 3.8] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Journal Information] [Submit a Manuscript] [Subscribe] [Scholar Register] [Received: 06/16/2020] [Revised: 09/05/2020] [Accepted: 09/22/2020] [Indexed: 02/06/2023] Open
Abstract
BACKGROUND Aceclofenac (ACF), a widely used nonsteroidal anti-inflammatory drug, has been associated with a number of severe cases of clinical hepatotoxicity. Terminalia bellirica, an evergreen tree, is known to have several ethnomedicinal uses including antioxidant and hepatoprotective effects. Hence T. bellirica fruit extracts and its phytoconstituent ellagic acid (EA) are expected to provide protection against oxidative stress and liver damage produced by long-term use of ACF.
AIM To evaluate the antioxidant and hepatoprotective activities of T. bellirica fruit extracts and EA against ACF-induced toxicity in albino Wistar rats.
METHODS The in vitro antioxidant activities of T. bellirica fruit ethyl acetate and aqueous extracts were measured by metal ion chelation and nitric oxide radical scavenging assays. The in vivo antioxidant and hepatoprotective effects of T. bellirica extracts (200 mg/kg) and EA (40 mg/kg) in ACF-induced hepatotoxic rats were assessed in serum and liver tissue after oral administration for 21 d. Silymarin (40 mg/kg) was used as a standard control. Oxidative stress markers in the blood (ferric reducing ability of plasma and lipid peroxidation inhibition) and liver tissues (superoxide dismutase, catalase and malondialdehyde) were analyzed using standard protocols. Liver function markers such as alkaline phosphatase, glutamic pyruvic transaminase, glutamic oxaloacetic transaminase, lactate dehydrogenase, γ-glutamyl transferase, creatinine, total protein, and uric acid were evaluated in rat serum.
RESULTS The T. bellirica fruit ethyl acetate extract exhibited superior metal ion chelating and nitric oxide radical scavenging abilities during in vitro antioxidant assays as compared to aqueous extracts. Oral administration of ACF in rats (15 mg/kg) for 21 d produced oxidative stress and adversely affected liver function suggesting liver injury. Treatment with extracts (ethyl acetate and aqueous), EA and silymarin accounted for a significant reduction in the adverse effects of ACF on oxidative stress and liver function markers in serum and hepatic tissue in rats. Histopathological evaluation of the liver indicated that the extracts and EA significantly decreased the degree of liver damage. The in vivo efficacy of EA was higher than T. bellirica fruit extracts. Of these extracts, ethyl acetate extract revealed comparatively better antioxidant and hepatoprotective activity.
CONCLUSION Ellagic acid and T. bellirica fruit extracts exhibited considerable hepatoprotective and antioxidant activities in long-term ACF-treated rats.
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Affiliation(s)
- Ashutosh Gupta
- Department of Biochemistry, University of Allahabad, Prayagraj 211002, Uttar Pradesh, India
| | - Abhay K Pandey
- Department of Biochemistry, University of Allahabad, Prayagraj 211002, Uttar Pradesh, India
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Das G, Kim DY, Fan C, Gutiérrez-Grijalva EP, Heredia JB, Nissapatorn V, Mitsuwan W, Pereira ML, Nawaz M, Siyadatpanah A, Norouzi R, Sawicka B, Shin HS, Patra JK. Plants of the Genus Terminalia: An Insight on Its Biological Potentials, Pre-Clinical and Clinical Studies. Front Pharmacol 2020; 11:561248. [PMID: 33132909 PMCID: PMC7578430 DOI: 10.3389/fphar.2020.561248] [Citation(s) in RCA: 9] [Impact Index Per Article: 1.8] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 05/12/2020] [Accepted: 09/07/2020] [Indexed: 12/16/2022] Open
Abstract
The evaluation and confirmation of healing properties of several plant species of genus Terminalia based on their traditional uses and the clinical claims are of utmost importance. Genus Terminalia has received more attention to assess and validate the therapeutic potential and clinical approval due to its immense folk medicinal and traditional applications. Various species of Terminalia genus are used in the form of herbal medicine and formulations, in treatment of diseases, including headache, fever, pneumonia, flu, geriatric, cancer, to improve memory, abdominal and back pain, cough and cold, conjunctivitis, diarrhea, heart disorder, leprosy, sexually transmitted diseases, and urinary tract disorders. These are reported to possess numerous biological properties, counting: antibacterial, antifungal, antiinflammatory, antiviral, antiretroviral, antioxidant, and antipa7rasitic. This current research review aims to update the detailed biological activities, pre-clinical and clinical studies of various extracts and secondary metabolites from several plant species under the genus Terminalia, along with information on the traditional uses and chemical composition to develop a promising strategy for their potential applications in the form of medicine or use in modern drug formulations for treating diseases like pneumonia, flu, and other types of viral infections or controlling human contagions.
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Affiliation(s)
- Gitishree Das
- Research Institute of Biotechnology & Medical Converged Science, Dongguk University-Seoul, Goyangsi, South Korea
| | - Do-Yeong Kim
- Research Institute of Biotechnology & Medical Converged Science, Dongguk University-Seoul, Goyangsi, South Korea
| | - Chen Fan
- Skin Research Institute of Singapore, Agency for Science, Technology and Research, A∗STAR, Singapore, Singapore
| | - Erick P. Gutiérrez-Grijalva
- Laboratorio de Alimentos Funcionales y Nutracéuticos, Cátedras CONACYT–Centro de Investigación en Alimentación y Desarrollo, Culiacán, México
| | - J. Basilio Heredia
- Laboratorio de Alimentos Funcionales y Nutracéuticos, Centro de Investigación en Alimentación y Desarrollo, Culiacán, México
| | - Veeranoot Nissapatorn
- School of Allied Health Sciences, Research Excellence Center for Innovation and Health Products (RECIHP) and World Union for Herbal Drugs Discovery (WUHeDD), Walailak University, Nakhon Si Thammarat, Thailand
| | - Watcharapong Mitsuwan
- School of Allied Health Sciences, Research Excellence Center for Innovation and Health Products (RECIHP) and World Union for Herbal Drugs Discovery (WUHeDD), Walailak University, Nakhon Si Thammarat, Thailand
| | - Maria Lourdes Pereira
- CICECO-Aveiro Institute of Materials and Department of Medical Sciences, University of Aveiro, Aveiro, Portugal
| | - Muhammad Nawaz
- Department of Nano-Medicine Research, Institute for Research and Medical Consultations (IRMC), Imam Abdulrahman Bin Faisal University, Dammam, Saudi Arabia
| | - Abolghasem Siyadatpanah
- Ferdows School of Paramedical and Health, Birjand University of Medical Sciences, Birjand, Iran
| | - Roghayeh Norouzi
- Department of Pathobiology, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran
| | - Barbara Sawicka
- Faculty of Agrobioengineering, Department of Plant Production Technology and Commodities Science, University of Life Sciences in Lublin, Lublin, Poland
| | - Han-Seung Shin
- Department of Food Science & Biotechnology, Dongguk University-Seoul, Goyangsi, South Korea
| | - Jayanta Kumar Patra
- Research Institute of Biotechnology & Medical Converged Science, Dongguk University-Seoul, Goyangsi, South Korea
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Tiwana G, Cock IE, White A, Cheesman MJ. Use of specific combinations of the triphala plant component extracts to potentiate the inhibition of gastrointestinal bacterial growth. JOURNAL OF ETHNOPHARMACOLOGY 2020; 260:112937. [PMID: 32464314 DOI: 10.1016/j.jep.2020.112937] [Citation(s) in RCA: 1] [Impact Index Per Article: 0.2] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Subscribe] [Scholar Register] [Received: 02/28/2020] [Revised: 04/28/2020] [Accepted: 04/29/2020] [Indexed: 06/11/2023]
Abstract
ETHNOPHARMACOLOGICAL RELEVANCE Triphala is used in Ayurveda to treat a wide variety of diseases, including numerous bacterial infections. Interestingly, the plant components of triphala (Terminalia bellirica, Terminalia chebula and Emblica officinalis) are also good inhibitors of bacterial growth when used individually, yet plant preparations are generally used in combination in traditional medicine. Surprisingly, no previous studies have addressed the reason why the combination is preferred over the individual components to treat bacterial infections. AIM OF THE STUDY To test and compare the antibacterial efficacy of triphala and its component parts to quantify their relative efficacies. The individual plant components will also be tested as combinations, thereby determining whether combining the individual components potentiates the antibacterial activity of the components used alone. MATERIALS AND METHODS Triphala and the three individual plant components were extracted using solvents of varying polarity (methanol, water, ethyl acetate) and the antibacterial activity of the aqueous resuspensions was quantified by disc diffusion and broth microdilution MIC assays. Combinations of extracts produced from the individual components were also tested against each bacterial species and the ΣFICs was calculated to determine the class of interaction. Where synergy was detected, isobologram analysis was used to determine the optimal component ratios. The Artemia nauplii bioassay was used to test for toxicity and GC-MS headspace profiling analysis was used to highlight terpenoid components that may contribute to the antibacterial activity of triphala. RESULTS The aqueous and methanolic triphala, T. bellirica, T. chebula and E. officinalis extracts displayed good inhibitory activity against all bacterial strains, with MICs often in the 250-750 μg/mL range. The methanolic extracts were generally more potent than the aqueous extracts and T. chebula was the most potent of the individual plant components. Combining the extracts of the different plant species resulted in potentiation of the growth inhibitory activity of most combinations compared to that of the individual components. Indeed, with the exception of S. flexneri, all bacterial species were potentiated by at least one combination of methanolic plant extracts, with a substantial proportion of these displaying synergistic interactions. All extracts were found to be either non-toxic, or of low to moderate toxicity in Artemia nauplii assays. CONCLUSION Whilst the individual plant components of triphala all inhibit the growth of multiple pathogenic bacteria, the activity is potentiated for multiple combinations. Therefore, the traditional usage of the combination of the three plant materials in triphala not only extends the activity profile of the mixture over that of the individual components, but it also substantially potentiates the inhibitory activity towards multiple bacteria, partially explaining the preference of triphala compared to the individual components.
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Affiliation(s)
- Gagan Tiwana
- School of Pharmacy and Pharmacology, Gold Coast Campus, Griffith University, Parklands Drive, Southport, Queensland, 4222, Australia; Menzies Health Institute Queensland, Quality Use of Medicines Network, Queensland, 4222, Australia
| | - Ian E Cock
- School of Environment and Science, Nathan Campus, Griffith University, 170 Kessels Rd, Nathan, Queensland, 4111, Australia; Environmental Futures Research Institute, Nathan Campus, Griffith University, 170 Kessels Rd, Nathan, Queensland, 4111, Australia.
| | - Alan White
- School of Environment and Science, Nathan Campus, Griffith University, 170 Kessels Rd, Nathan, Queensland, 4111, Australia
| | - Matthew J Cheesman
- School of Pharmacy and Pharmacology, Gold Coast Campus, Griffith University, Parklands Drive, Southport, Queensland, 4222, Australia; Menzies Health Institute Queensland, Quality Use of Medicines Network, Queensland, 4222, Australia.
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Gupta A, Kumar R, Bhattacharyya P, Bishayee A, Pandey AK. Terminalia bellirica (Gaertn.) roxb. (Bahera) in health and disease: A systematic and comprehensive review. PHYTOMEDICINE : INTERNATIONAL JOURNAL OF PHYTOTHERAPY AND PHYTOPHARMACOLOGY 2020; 77:153278. [PMID: 32781393 DOI: 10.1016/j.phymed.2020.153278] [Citation(s) in RCA: 31] [Impact Index Per Article: 6.2] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Subscribe] [Scholar Register] [Received: 04/16/2020] [Revised: 06/18/2020] [Accepted: 07/04/2020] [Indexed: 06/11/2023]
Abstract
BACKGROUND Terminalia bellirica (Gaertn.) Roxb. is one of the oldest medicinal herbs of India, Pakistan, Nepal, Bangladesh and Sri Lanka as well as South-East Asia. Its medicinal utility has been described in the different traditional medicinal systems, such as Ayurveda, Unani, Siddha, and traditional Chinese medicine. PURPOSE The present study is aimed at providing a comprehensive overview on the traditional medicinal use, major phytoconstituents, biological and pharmacological activities and related mechanisms of actions and clinical studies of T. bellirica. Another objective is to describe current limitations and future direction of T. bellirica-related research. METHODS PubMed, ScienceDirect, Scopus, Cochrane Library, and EBOSCO host databases were selected to explore literature published between 1980 and 2020 (till March). Keywords used in various combinations comprised of Terminalia bellirica, phytoconstituents, health effects, pharmacological activities, molecular targets, in vitro, in vivo, clinical studies, and disease prevention. RESULTS A broad spectrum in vitro and in vivo studies suggested various biological and pharmacological effects, including antioxidant, anti-inflammatory, immunomodulatory, antimicrobial, hepatoprotective, renoprotective, antidiabetic, anti-hyperlipidemic, and anticancer activities. Diverse bioactivities of T. bellirica have been ascribed to the presence of many bioactive phytochemicals, such as glucoside, tannins, gallic acid, corilagin, ellagic acid, ethyl gallate, galloyl glucose, chebulagic acid, and arjunolic acid. CONCLUSION Preclinical and clinical studies have suggested that T. bellirica plant and its phytoconstituents have immense potential for prevention and treatment of various diseases. Additional in vivo studies and clinical trials are warranted to realize the complete medicinal attributes of this plant.
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Affiliation(s)
- Ashutosh Gupta
- Department of Biochemistry, University of Allahabad, Prayagraj - 211 002, Uttar Pradesh, India
| | - Ramesh Kumar
- Department of Biochemistry, University of Allahabad, Prayagraj - 211 002, Uttar Pradesh, India
| | - Piyali Bhattacharyya
- Department of Nutrition, School of Health Sciences, Ana G. Méndez University, Gurabo, PR 00778, United States
| | - Anupam Bishayee
- Lake Erie College of Osteopathic Medicine, Bradenton, FL 34211, United States.
| | - Abhay K Pandey
- Department of Biochemistry, University of Allahabad, Prayagraj - 211 002, Uttar Pradesh, India.
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Mi S, Yu W, Li J, Liu M, Sang Y, Wang X. Characterization and discrimination of chilli peppers based on multi-element and non-targeted metabolomics analysis. Lebensm Wiss Technol 2020. [DOI: 10.1016/j.lwt.2020.109742] [Citation(s) in RCA: 6] [Impact Index Per Article: 1.2] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 12/17/2022]
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Ohara R, Périco LL, Rodrigues VP, Bueno G, Zanatta AC, Campaner Dos Santos L, Vilegas W, Constatino FB, Justulin LA, Hiruma-Lima CA. Terminalia catappa L. infusion accelerates the healing process of gastric ischemia-reperfusion injury in rats. JOURNAL OF ETHNOPHARMACOLOGY 2020; 256:112793. [PMID: 32240780 DOI: 10.1016/j.jep.2020.112793] [Citation(s) in RCA: 6] [Impact Index Per Article: 1.2] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Subscribe] [Scholar Register] [Received: 02/03/2020] [Revised: 03/18/2020] [Accepted: 03/22/2020] [Indexed: 06/11/2023]
Abstract
ETHNOPHARMACOLOGICAL RELEVANCE Terminalia catappa L. (Combretaceae), known as "amendoeira da praia" in Brazil, has been recognized as a medicinal plant in folk medicine for the treatment of gastrointestinal disorders and other inflammatory conditions. The present study aimed to investigate the preventive and healing effects of the infusion of leaves of T. catappa (ILTC) against gastric lesions caused by ischemia and reperfusion (I/R) injury and characterize its mechanism of action in the gastric mucosa of rats. MATERIALS AND METHODS Different doses (30, 100, and 300 mg/kg) of ILTC were orally administered as acute and subacute treatments against I/R-induced gastric lesion in rats. After treatment, the stomach of rats was collected to measure the lesion area, redox parameters malondialdehyde (MDA), superoxide dismutase (SOD), catalase (CAT), and reduced glutathione (GSH) and inflammatory parameters myeloperoxidase activity (MPO), interleukin 1β (IL-1β) and tumor necrosis factor α (TNF-α). The activities of matrix metalloproteinases 2 and 9 (MMPs 2 and 9) were assessed by zymography method to clarify the mechanisms of the healing acceleration promoted by ILTC. RESULTS Pretreatment with ILTC (100 mg/kg) was effective in preventing the aggravation of lesions in the acute model by reducing MPO activity by 38% relative to control group, despite the lack of clarity of this action at the macroscopical level at the lesion area (p < 0.05). After three days of treatment with ILTC (30 and 100 mg/kg), this infusion significantly reduced the lesion area by 95% and 89%, respectively, compared the control (p < 0.05). The gastric healing effect of all doses of ILTC was followed by a reduction in MPO activity (decrease by 70-78%). Compared to the negative control, an improvement in gastric healing owing to treatment with ILTC was observed and this was followed by an increase in MMP-2 (20-47%) (p < 0.05). CONCLUSION Three days of treatment with ILTC could accelerate the healing process in I/R-induced lesions in rats. By decreasing MPO levels, ILTC enabled the action of MMP-2, which led to tissue recovery in the gastric mucosa.
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Affiliation(s)
- Rie Ohara
- Department of Physiology, Biosciences Institute, UNESP-São Paulo State University, CEP 18618-689, Botucatu, São Paulo, Brazil
| | - Larissa Lucena Périco
- Department of Physiology, Biosciences Institute, UNESP-São Paulo State University, CEP 18618-689, Botucatu, São Paulo, Brazil; Snyder Institute for Chronic Diseases, Cumming School of Medicine, University of Calgary, Alberta, Canada
| | - Vinicius Peixoto Rodrigues
- Department of Physiology, Biosciences Institute, UNESP-São Paulo State University, CEP 18618-689, Botucatu, São Paulo, Brazil
| | - Gabriela Bueno
- Department of Physiology, Biosciences Institute, UNESP-São Paulo State University, CEP 18618-689, Botucatu, São Paulo, Brazil
| | - Ana Caroline Zanatta
- Biosciences Institute, UNESP-São Paulo State University, CEP 11330-900, São Vicente, São Paulo, Brazil; Institute of Chemistry, UNESP-São Paulo State University, CEP 14800-900, Araraquara, São Paulo, Brazil
| | | | - Wagner Vilegas
- Biosciences Institute, UNESP-São Paulo State University, CEP 11330-900, São Vicente, São Paulo, Brazil
| | - Flavia Bessi Constatino
- Laboratory of Extracellular Matrix, UNESP- São Paulo State University, CEP 18618-689, Botucatu, São Paulo, Brazil
| | - Luis Antonio Justulin
- Laboratory of Extracellular Matrix, UNESP- São Paulo State University, CEP 18618-689, Botucatu, São Paulo, Brazil
| | - Clélia Akiko Hiruma-Lima
- Department of Physiology, Biosciences Institute, UNESP-São Paulo State University, CEP 18618-689, Botucatu, São Paulo, Brazil.
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Nigam M, Mishra AP, Adhikari-Devkota A, Dirar AI, Hassan MM, Adhikari A, Belwal T, Devkota HP. Fruits of Terminalia chebula Retz.: A review on traditional uses, bioactive chemical constituents and pharmacological activities. Phytother Res 2020; 34:2518-2533. [PMID: 32307775 DOI: 10.1002/ptr.6702] [Citation(s) in RCA: 51] [Impact Index Per Article: 10.2] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 08/08/2019] [Revised: 02/29/2020] [Accepted: 03/28/2020] [Indexed: 11/12/2022]
Abstract
Fruits of Terminalia chebula Retz. (Combretaceae) are widely used as crude drugs in various traditional medicine systems. The aim of this article is to review the available scientific information regarding the traditional uses, bioactive chemical constituents and the pharmacological activities of T. chebula. Numerous researches conducted on T. chebula have confirmed the presence of wide range of the phytochemicals such as flavonoids, tannins, phenolic acids and other bioactive compounds. T. chebula is also widely studied regarding its pharmacological activities such as antioxidant, hepatoprotective, neuroprotective, cytotoxic, antidiabetic, anti-inflammatory activities among others. However, more in vivo and clinical studies for mechanism-based pharmacological evaluation should be conducted in future to provide stronger scientific evidences for their traditional uses.
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Affiliation(s)
- Manisha Nigam
- Department of Biochemistry, H. N. B. Garhwal University, Srinagar Garhwal, India
| | - Abhay P Mishra
- Department of Pharmaceutical Chemistry, H. N. B. Garhwal University, Srinagar Garhwal, India
| | | | - Amina Ibrahim Dirar
- Graduate School of Pharmaceutical Sciences, Kumamoto University, Kumamoto, Japan
| | - Md Mahadi Hassan
- Graduate School of Pharmaceutical Sciences, Kumamoto University, Kumamoto, Japan
| | - Achyut Adhikari
- Central Department of Chemistry, Tribhuwan University, Kritipur, Nepal
| | - Tarun Belwal
- Key Laboratory for Agro-Products Postharvest Handling of Ministry of Agriculture and Rural Affairs, Zhejiang Key Laboratory for Agro-Food Processing, College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou, China
| | - Hari Prasad Devkota
- Graduate School of Pharmaceutical Sciences, Kumamoto University, Kumamoto, Japan.,Program for Leading Graduate Schools, Health Life Science: Interdisciplinary and Glocal Oriented (HIGO) Program, Kumamoto University, Kumamoto, Japan
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Cock IE, Van Vuuren SF. A review of the traditional use of southern African medicinal plants for the treatment of fungal skin infections. JOURNAL OF ETHNOPHARMACOLOGY 2020; 251:112539. [PMID: 31899200 DOI: 10.1016/j.jep.2019.112539] [Citation(s) in RCA: 4] [Impact Index Per Article: 0.8] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Subscribe] [Scholar Register] [Received: 11/22/2019] [Revised: 12/23/2019] [Accepted: 12/30/2019] [Indexed: 06/10/2023]
Abstract
ETHNOPHARMACOLOGICAL RELEVANCE Human dermatophyte infections are one of the most common classes of infection globally, with an estimated 1.7 billion people contracting at least one infection annually. Southern African ethnic groups used multiple plants to treat dermatophytosis and to alleviate the symptoms, yet the anti-dermatophyte properties of most species remain poorly explored. AIM OF THE STUDY Our study aimed to critically review the literature to document southern African plant species used to treat one or more dermatophytic infections, and to summarise scientific evaluations of these and other plant species. Our study aims to stimulate and focus future studies in this field. MATERIALS AND METHODS A thorough review of the ethnobotanical books, reviews and primary scientific studies were undertaken to identify southern African plants used traditionally to treat dermatophytosis, thereby identifying gaps in the research requiring further study. RESULTS Eighty-nine southern African plant species are recorded as traditional therapies for dermatophytosis. Scientific evaluations of 140 plant species were identified, although most of the species in those studies were selected for reasons apart from their traditional uses. None of those studies examined the mechanism of action of the plant species, and only a single study screened the extracts for toxicity. CONCLUSIONS Despite southern Africa having some of the longest continuous human civilisations globally, as well as unique and diverse flora, and good ethnobotanical records, the anti-dermatophyte properties of southern African medicinal plants remains relatively poorly explored. The efficacy of the majority of plants used traditionally to treat fungal skin disease are yet to be verified and substantial further research is required in this field.
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Affiliation(s)
- I E Cock
- School of Environment and Science, Nathan Campus, Griffith University, 170 Kessels Rd, Nathan, Queensland, 4111, Australia; Environmental Futures Research Institute, Nathan Campus, Griffith University, 170 Kessels Rd, Nathan, Queensland, 4111, Australia.
| | - S F Van Vuuren
- Department of Pharmacy and Pharmacology, Faculty of Health Sciences, University of the Witwatersrand, Parktown, Gauteng, 2193, South Africa
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Moghaddam M, Mehdizadeh L, Sharifi Z. Macro- and microelement content and health risk assessment of heavy metals in various herbs of Iran. ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH INTERNATIONAL 2020; 27:12320-12331. [PMID: 31993898 DOI: 10.1007/s11356-020-07789-2] [Citation(s) in RCA: 19] [Impact Index Per Article: 3.8] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Grants] [Track Full Text] [Subscribe] [Scholar Register] [Received: 07/04/2019] [Accepted: 01/21/2020] [Indexed: 06/10/2023]
Abstract
In this study, element content and health risk of the most popular herbs from Iran were evaluated. The samples of raw materials from 30 different herbs were purchased from the local markets of Iran. The concentration levels of some elements including macroelements (N, P, K, Ca, and Mg), micronutrients (Fe, Zn, Cu, Mn, and Na), and heavy metals (Cd, Ni, and Pb) of studied herbs were evaluated. The potential of health risks was calculated by Estimated Daily Intake (EDI), Target Hazard Quotient (THQ), and Hazard Index (HI). The analysis of variance (ANOVA) is used to test a hypothesis about differences between the mean values. The highest levels of Ca (20,000 ± 26.3 mg/kg), Mg (9600 ± 45.4 mg/kg), N (59,955 ± 11.55 mg/kg), P (6544 ± 20 mg/kg), and K (56,563.2 ± 18 mg/kg) were found in Zataria multiflora, Malva sylvestris, Acasia arbus, Cannabis sativa, and Amomum subulatum, respectively. In addition, the highest concentration levels of Fe (987 ± 75.27 mg/kg), Zn (1187.5 ± 10 mg/kg), Cu (64.2 ± 2 mg/kg), Mn (272.3 ± 66.62 mg/kg), and Na (2658.8 ± 20.3 mg/kg) were recorded in Bunium persicum, Peganum harmala, Papaver somniferum, Alpinia officinalis, and Cuminum cyminum, respectively. Acasia arbus, Anethum graveolens, and Malva sylvestris showed the highest concentration of Ni (6.07 ± 0.04 mg/kg), Cd (1.64 ± 0.16 mg/kg), and Pb (9.27 ± 0.25 mg/kg). Based on performed health risk assessment on the studied plants, EDI, THQ, and HI values of all of them were less than 1. This study indicated that there were several harmful elements in the herbs. The healthier plant species are those with the least concentration of Pb, Ni, and Cd, which include Vitex agnus-custus and Teucrium polium. On the other hand, the toxic plants with a higher concentration of Pb, Ni, and Cd included Malva sylvestris, Acasia arbus, and Anethum graveolens. In addition, evaluation of human risk assessment is an important factor for investigating the concentration of heavy metals harmful for human beings.
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Affiliation(s)
- Mohammad Moghaddam
- Department of Horticulture Science, Faculty of Agriculture, Ferdowsi University of Mashhad, P.O. Box 91775-1163, Mashhad, Iran.
| | - Leila Mehdizadeh
- Department of Horticulture Science, Faculty of Agriculture, Ferdowsi University of Mashhad, P.O. Box 91775-1163, Mashhad, Iran
| | - Zahra Sharifi
- Department of Soil Science, Faculty of Agriculture, Ferdowsi University of Mashhad, P.O. Box 91775-1163, Mashhad, Iran
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Effect of Herbal Formulation on Immune Response Enhancement in RAW 264.7 Macrophages. Biomolecules 2020; 10:biom10030424. [PMID: 32182890 PMCID: PMC7175197 DOI: 10.3390/biom10030424] [Citation(s) in RCA: 9] [Impact Index Per Article: 1.8] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 01/29/2020] [Revised: 02/27/2020] [Accepted: 03/02/2020] [Indexed: 02/07/2023] Open
Abstract
Immune response is a necessary self-defense mechanism that protects the host from infectious organisms. Many medicinal plants are popularly used in Asian folk medicine to increase body resistance. An herbal formulation named KM1608 was prepared from three medicinal plants: Saussurea lappa, Terminalia chebula, and Zingiber officinale. In this study, we evaluated the immune stimulatory effect of KM1608 on RAW 264.7 murine macrophages. Network pharmacological analyses were used to predict potential immune response pathways of major compounds from KM1608. The cytotoxicity and immuno-stimulating effect of KM1608 were determined using cell viability and nitric oxide assays. The underlying mechanism of immunomodulatory activity was evaluated by quantitative real-time reverse transcription polymerase chain reaction (qRT-PCR) of pro-inflammatory cytokines. The results of network pharmacological analysis suggested that major compounds from KM1608 possess anticancer potential via immune signaling pathways. After treatment with KM1608 at 25–100 µg/mL for 24 h, the level of nitric oxide was increased in the dose-dependent manner. The results of quantitative real-time PCR showed that KM1608 stimulates the expression of immune cytokines (interferon (IFN)-α, -β, IL-1β, -6, IL-10, inducible nitric oxide synthase (iNOS), and cyclooxygenase-2 (COX-2)) in macrophages. KM1608 extract is a potential agent for immune response enhancement.
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