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Wang H, Sanghvi G, Arefpour A, Alkhayyat A, Soheily A, Jabbarzare S, Salahshour S, Alizadeh A, Baghaei S. Using hardystonite as a biomaterial in biomedical and bone tissue engineering applications. Tissue Cell 2024; 91:102551. [PMID: 39255743 DOI: 10.1016/j.tice.2024.102551] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 02/08/2024] [Revised: 08/28/2024] [Accepted: 08/30/2024] [Indexed: 09/12/2024]
Abstract
Widespread adoption for substitutes of artificial bone grafts based on proper bioceramics has been generated in recent years. Among them, calcium-silicate-based bioceramics, which possess osteoconductive properties and can directly attach to biological organs, have attracted substantial attention for broad ranges of applications in bone tissue engineering. Approaches exist for a novel strategy to promote the drawbacks of bioceramics such as the incorporation of Zn2+, Mg2+, and Zr4+ ions into calcium-silicate networks, and the improvement of their physical, mechanical, and biological properties. Recently, hardystonite (Ca2ZnSi2O7) bioceramics, as one of the most proper calcium-silicate-based bioceramics, has presented excellent biocompatibility, bioactivity, and interaction. Due to its physical, mechanical, and biological behaviors and ability to be shaped utilizing a variety of fabrication techniques, hardystonite possesses the potential to be applied in biomedical and tissue engineering, mainly bone tissue engineering. A notable potential exists for the newly developed bioceramics to help therapies supply clinical outputs. The promising review paper has been presented by considering major aims to summarize and discuss the most applicable studies carried out for its physical, mechanical, and biological behaviors.
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Affiliation(s)
- Haoyu Wang
- Medical College, Xijing University, Xi'an, Shaanxi 710123, China; Department of Orthopedics, The Second Affiliated Hospital, Xi'an Jiaotong University, Xi'an, Shaanxi 710004, China.
| | - Gaurav Sanghvi
- Marwadi University Research Center, Department of Microbiology, Faculty of Science, Marwadi University, Rajkot, Gujarat 360003, India
| | - Ahmadreza Arefpour
- Department of Materials Engineering, Isfahan University of Technology, Isfahan 84156-83111, Iran
| | - Ahmad Alkhayyat
- Department of computers Techniques engineering, College of technical engineering, The Islamic University, Najaf, Iraq; Department of computers Techniques engineering, College of technical engineering, The Islamic University of Al Diwaniyah, Al Diwaniyah, Iraq; Department of computers Techniques engineering, College of technical engineering, The Islamic University of Babylon, Babylon, Iraq
| | - Ali Soheily
- Advanced Materials Research Center, Department of Materials Engineering, Najafabad Branch, Islamic Azad University, Najafabad, Iran
| | - Saeid Jabbarzare
- Advanced Materials Research Center, Department of Materials Engineering, Najafabad Branch, Islamic Azad University, Najafabad, Iran
| | - Soheil Salahshour
- Faculty of Engineering and Natural Sciences, Istanbul Okan University, Istanbul, Turkey; Faculty of Engineering and Natural Sciences, Bahcesehir University, Istanbul, Turkey; Department of Computer Science and Mathematics, Lebanese American University, Beirut, Lebanon
| | - As'ad Alizadeh
- Department of Mechanical Engineering, College of Engineering, Urmia University, Urmia, Iran
| | - Sh Baghaei
- Department of mechanical engineering, Khomeinishahr branch, Islamic Azad University, Khomeinishahr, Iran.
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Khalvandi A, Saber-Samandari S, Aghdam MM. A supervised learning-assisted multi-scale study for thermal and mechanical behavior of porous Silica. Heliyon 2024; 10:e28995. [PMID: 38633647 PMCID: PMC11021964 DOI: 10.1016/j.heliyon.2024.e28995] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 10/18/2023] [Revised: 03/23/2024] [Accepted: 03/27/2024] [Indexed: 04/19/2024] Open
Abstract
This paper presents a comprehensive investigation of mesoporous Silica utilizing a multi-scale modeling approach under periodic boundary conditions integrated with machine learning algorithms. The study begins with Molecular Dynamics (MD) simulations to extract Silica's elastic properties and thermal conductivity at the nano-scale, employing the Tersoff potential. Subsequently, the derived material characteristics are applied to a series of generated porous Representative Volume Elements (RVEs) at the microscale. This phase involves the exploration of porosity and void shape effects on Silica's thermal and mechanical properties, considering inhomogeneities' distributions along the X-axis and random dispersion of pore cells within a three-dimensional space. Furthermore, the influence of pore shape is examined by defining open and closed-cell models, encompassing spherical and ellipsoidal voids with aspect ratios of 2 and 4. To predict the properties of porous Silica, a shallow Artificial Neural Network (ANN) is deployed, utilizing geometric parameters of the RVEs and porosity. Subsequently, it is revealed that Silica's thermal and mechanical behavior is linked to pore geometry, distribution, and porosity model. Finally, to classify the behavior of porous Silica into three categories, quasi-isotropic, orthotropic, and transversely-isotropic, three methodologies of decision tree approach, K-Nearest Neighbors (KNN) algorithm, and Support Vector Machines (SVMs) are employed. Among these, SVMs employing a quadratic kernel function demonstrate robust performance in categorizing the thermal and mechanical behavior of porous Silica.
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Affiliation(s)
- Ali Khalvandi
- Department of Mechanical Engineering, Amirkabir University of Technology, Tehran, Iran
- Composites Research Laboratory (CRLab), Amirkabir University of Technology, Tehran, Iran
- New Technologies Research Center, Amirkabir University of Technology, Tehran, Iran
| | - Saeed Saber-Samandari
- Composites Research Laboratory (CRLab), Amirkabir University of Technology, Tehran, Iran
- New Technologies Research Center, Amirkabir University of Technology, Tehran, Iran
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Wang X, Fu X, Luo D, Hou R, Li P, Chen Y, Zhang X, Meng X, Yue Y, Liu J. 3D printed high-precision porous scaffolds prepared by fused deposition modeling induce macrophage polarization to promote bone regeneration. Biomed Mater 2024; 19:035006. [PMID: 38422525 DOI: 10.1088/1748-605x/ad2ed0] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 10/31/2023] [Accepted: 02/29/2024] [Indexed: 03/02/2024]
Abstract
Macrophage-mediated bone immune responses significantly influence the repair of bone defects when utilizing tissue-engineered scaffolds. Notably, the scaffolds' physical structure critically impacts macrophage polarization. The optimal pore size for facilitating bone repair remains a topic of debate due to the imprecision of traditional methods in controlling scaffold pore dimensions and spatial architecture. In this investigation, we utilized fused deposition modeling (FDM) technology to fabricate high-precision porous polycaprolactone (PCL) scaffolds, aiming to elucidate the impact of pore size on macrophage polarization. We assessed the scaffolds' mechanical attributes and biocompatibility. Real-time quantitative reverse transcription polymerase chain reaction was used to detect the expression levels of macrophage-related genes, and enzyme linked immunosorbent assay for cytokine secretion levels.In vitroosteogenic capacity was determined through alkaline phosphatase and alizarin red staining. Our findings indicated that macroporous scaffolds enhanced macrophage adhesion and drove their differentiation towards the M2 phenotype. This led to the increased production of anti-inflammatory factors and a reduction in pro-inflammatory agents, highlighting the scaffolds' immunomodulatory capabilities. Moreover, conditioned media from macrophages cultured on these macroporous scaffolds bolstered the osteogenic differentiation of bone marrow mesenchymal stem cells, exhibiting superior osteogenic differentiation potential. Consequently, FDM-fabricated PCL scaffolds, with precision-controlled pore sizes, present promising prospects as superior materials for bone tissue engineering, leveraging the regulation of macrophage polarization.
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Affiliation(s)
- Xiangyu Wang
- Shanxi Medical University School and Hospital of Stomatology, Taiyuan 030001, People's Republic of China
- Shanxi Province Key Laboratory of Oral Diseases Prevention and New Materials, Taiyuan 030001, People's Republic of China
| | - Xinyu Fu
- Shanxi Medical University School and Hospital of Stomatology, Taiyuan 030001, People's Republic of China
- Shanxi Province Key Laboratory of Oral Diseases Prevention and New Materials, Taiyuan 030001, People's Republic of China
| | - Dongmei Luo
- Shanxi Medical University School and Hospital of Stomatology, Taiyuan 030001, People's Republic of China
- Shanxi Province Key Laboratory of Oral Diseases Prevention and New Materials, Taiyuan 030001, People's Republic of China
| | - Ruxia Hou
- Shanxi Medical University School and Hospital of Stomatology, Taiyuan 030001, People's Republic of China
- Shanxi Province Key Laboratory of Oral Diseases Prevention and New Materials, Taiyuan 030001, People's Republic of China
| | - Peiwen Li
- Shanxi Medical University School and Hospital of Stomatology, Taiyuan 030001, People's Republic of China
- Shanxi Province Key Laboratory of Oral Diseases Prevention and New Materials, Taiyuan 030001, People's Republic of China
| | - Yurou Chen
- Shanxi Medical University School and Hospital of Stomatology, Taiyuan 030001, People's Republic of China
- Shanxi Province Key Laboratory of Oral Diseases Prevention and New Materials, Taiyuan 030001, People's Republic of China
| | - Xinyao Zhang
- Shanxi Medical University School and Hospital of Stomatology, Taiyuan 030001, People's Republic of China
- Shanxi Province Key Laboratory of Oral Diseases Prevention and New Materials, Taiyuan 030001, People's Republic of China
| | - Xiangjie Meng
- Shanxi Medical University School and Hospital of Stomatology, Taiyuan 030001, People's Republic of China
- Shanxi Province Key Laboratory of Oral Diseases Prevention and New Materials, Taiyuan 030001, People's Republic of China
| | - Yingge Yue
- Shanxi Medical University School and Hospital of Stomatology, Taiyuan 030001, People's Republic of China
- Shanxi Province Key Laboratory of Oral Diseases Prevention and New Materials, Taiyuan 030001, People's Republic of China
| | - Junyu Liu
- Shanxi Medical University School and Hospital of Stomatology, Taiyuan 030001, People's Republic of China
- Shanxi Province Key Laboratory of Oral Diseases Prevention and New Materials, Taiyuan 030001, People's Republic of China
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Koplányi G, Bell E, Molnár Z, Katona G, Lajos Neumann P, Ender F, Balogh GT, Žnidaršič-Plazl P, Poppe L, Balogh-Weiser D. Novel Approach for the Isolation and Immobilization of a Recombinant Transaminase: Applying an Advanced Nanocomposite System. Chembiochem 2023; 24:e202200713. [PMID: 36653306 DOI: 10.1002/cbic.202200713] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 12/02/2022] [Revised: 01/18/2023] [Accepted: 01/18/2023] [Indexed: 01/20/2023]
Abstract
The increasing application of recombinant enzymes demands not only effective and sustainable fermentation, but also highly efficient downstream processing and further stabilization of the enzymes by immobilization. In this study, a novel approach for the isolation and immobilization of His-tagged transaminase from Chromobacterium violaceum (CvTA) has been developed. A recombinant of CvTA was simultaneously isolated and immobilized by binding on silica nanoparticles (SNPs) with metal affinity linkers and additionally within poly(lactic acid) (PLA) nanofibers. The linker length and the nature of the metal ion significantly affected the enzyme binding efficiency and biocatalytic activity of CvTA-SNPs. The formation of PLA nanofibers by electrospinning enabled rapid embedding of CvTA-SNPs biocatalysts and ensured enhanced stability and activity. The developed advanced immobilization method reduces the time required for enzyme isolation, purification and immobilization by more than fourfold compared to a classical stepwise technique.
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Affiliation(s)
- Gábor Koplányi
- Department of Organic Chemistry and Technology, Budapest University of Technology and Economics, 1111, Műegyetem rkp. 3., Budapest, Hungary
| | - Evelin Bell
- Department of Organic Chemistry and Technology, Budapest University of Technology and Economics, 1111, Műegyetem rkp. 3., Budapest, Hungary
| | - Zsófia Molnár
- Department of Organic Chemistry and Technology, Budapest University of Technology and Economics, 1111, Műegyetem rkp. 3., Budapest, Hungary.,Institute of Enzymology, ELKH Research Center of Natural Sciences, 1117, Magyar tudosók krt. 2. Budapest, Hungary
| | - Gábor Katona
- Institute of Pharmaceutical Technology and Regulatory Affairs, University of Szeged, 6720, Eötvös u. 6., Szeged, Hungary
| | - Péter Lajos Neumann
- Department of Electron Devices, Budapest University of Technology and Economics, 1111, Műegyetem rkp. 3., Budapest, Hungary.,Centre for Energy Research, Institute for Technical Physics and Materials Science, 1121, Konkoly-Thege M. út 29-33., Budapest, Hungary
| | - Ferenc Ender
- Department of Electron Devices, Budapest University of Technology and Economics, 1111, Műegyetem rkp. 3., Budapest, Hungary.,SpinSplit Llc., 1025, Vend u. 17., Budapest, Hungary
| | - György T Balogh
- Department of Chemical and Environmental Process Engineering, Budapest University of Technology and Economics, 1111, Műegyetem rkp. 3., Budapest, Hungary.,Institute of Pharmacodynamics and Biopharmacy, University of Szeged, 6720, Eötvös u. 6., Szeged, Hungary
| | - Polona Žnidaršič-Plazl
- Faculty of Chemistry and Chemical Technology, University of Ljubljana Večna pot 113., 1000, Ljubljana, Slovenia
| | - László Poppe
- Department of Organic Chemistry and Technology, Budapest University of Technology and Economics, 1111, Műegyetem rkp. 3., Budapest, Hungary.,Biocatalysis and Biotransformation Research Center Faculty of Chemistry and Chemical Engineering, Babeş-Bolyai University of Cluj-Napoca, 400028, Arany János Str. 11, Cluj-Napoca, Romania
| | - Diána Balogh-Weiser
- Department of Organic Chemistry and Technology, Budapest University of Technology and Economics, 1111, Műegyetem rkp. 3., Budapest, Hungary.,Department of Physical Chemistry and Materials Science, Budapest University of Technology and Economics, 1111, Műegyetem rkp. 3., Budapest, Hungary
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Cui J, Yu X, Shen Y, Sun B, Guo W, Liu M, Chen Y, Wang L, Zhou X, Shafiq M, Mo X. Electrospinning Inorganic Nanomaterials to Fabricate Bionanocomposites for Soft and Hard Tissue Repair. NANOMATERIALS (BASEL, SWITZERLAND) 2023; 13:204. [PMID: 36616113 PMCID: PMC9823959 DOI: 10.3390/nano13010204] [Citation(s) in RCA: 12] [Impact Index Per Article: 6.0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Figures] [Subscribe] [Scholar Register] [Received: 11/30/2022] [Revised: 12/27/2022] [Accepted: 12/29/2022] [Indexed: 06/17/2023]
Abstract
Tissue engineering (TE) has attracted the widespread attention of the research community as a method of producing patient-specific tissue constructs for the repair and replacement of injured tissues. To date, different types of scaffold materials have been developed for various tissues and organs. The choice of scaffold material should take into consideration whether the mechanical properties, biodegradability, biocompatibility, and bioresorbability meet the physiological properties of the tissues. Owing to their broad range of physico-chemical properties, inorganic materials can induce a series of biological responses as scaffold fillers, which render them a good alternative to scaffold materials for tissue engineering (TE). While it is of worth to further explore mechanistic insight into the use of inorganic nanomaterials for tissue repair, in this review, we mainly focused on the utilization forms and strategies for fabricating electrospun membranes containing inorganic components based on electrospinning technology. A particular emphasis has been placed on the biological advantages of incorporating inorganic materials along with organic materials as scaffold constituents for tissue repair. As well as widely exploited natural and synthetic polymers, inorganic nanomaterials offer an enticing platform to further modulate the properties of composite scaffolds, which may help further broaden the application prospect of scaffolds for TE.
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Affiliation(s)
- Jie Cui
- State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, Shanghai Engineering Research Center of Nano-Biomaterials and Regenerative Medicine, College of Biological Science and Medical Engineering, Donghua University, Shanghai 201620, China
| | - Xiao Yu
- State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, Shanghai Engineering Research Center of Nano-Biomaterials and Regenerative Medicine, College of Biological Science and Medical Engineering, Donghua University, Shanghai 201620, China
| | - Yihong Shen
- State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, Shanghai Engineering Research Center of Nano-Biomaterials and Regenerative Medicine, College of Biological Science and Medical Engineering, Donghua University, Shanghai 201620, China
| | - Binbin Sun
- State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, Shanghai Engineering Research Center of Nano-Biomaterials and Regenerative Medicine, College of Biological Science and Medical Engineering, Donghua University, Shanghai 201620, China
| | - Wanxin Guo
- State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, Shanghai Engineering Research Center of Nano-Biomaterials and Regenerative Medicine, College of Biological Science and Medical Engineering, Donghua University, Shanghai 201620, China
| | - Mingyue Liu
- State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, Shanghai Engineering Research Center of Nano-Biomaterials and Regenerative Medicine, College of Biological Science and Medical Engineering, Donghua University, Shanghai 201620, China
| | - Yujie Chen
- State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, Shanghai Engineering Research Center of Nano-Biomaterials and Regenerative Medicine, College of Biological Science and Medical Engineering, Donghua University, Shanghai 201620, China
| | - Li Wang
- College of Science, Donghua University, Shanghai 201620, China
| | - Xingping Zhou
- State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, Shanghai Engineering Research Center of Nano-Biomaterials and Regenerative Medicine, College of Biological Science and Medical Engineering, Donghua University, Shanghai 201620, China
| | - Muhammad Shafiq
- State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, Shanghai Engineering Research Center of Nano-Biomaterials and Regenerative Medicine, College of Biological Science and Medical Engineering, Donghua University, Shanghai 201620, China
- Department of Chemical Engineering, Faculty of Engineering, Graduate School, Kyushu University, 744 Motooka, Nishi-Ku, Fukuoka 819-0395, Japan
- Department of Biotechnology, Faculty of Science and Technology (FOST), University of Central Punjab (UCP), Lahore 54000, Pakistan
| | - Xiumei Mo
- State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, Shanghai Engineering Research Center of Nano-Biomaterials and Regenerative Medicine, College of Biological Science and Medical Engineering, Donghua University, Shanghai 201620, China
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Oustadi F, Imani R, Haghbin Nazarpak M, Sharifi AM, McInnes SJP. Nanofiber/hydrogel composite scaffold incorporated by silicon nanoparticles for sustained delivery of osteogenic factor: in vitro study. INT J POLYM MATER PO 2022. [DOI: 10.1080/00914037.2022.2147176] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 11/27/2022]
Affiliation(s)
- Fereshteh Oustadi
- Department of Biomedical Engineering, Amirkabir University of Technology (Tehran Polytechnic), Tehran, Iran
| | - Rana Imani
- Department of Biomedical Engineering, Amirkabir University of Technology (Tehran Polytechnic), Tehran, Iran
| | - Masoumeh Haghbin Nazarpak
- New Technologies Research Center, Amirkabir University of Technology (Tehran Polytechnic), Tehran, Iran
| | - Ali Mohammad Sharifi
- Stem Cell and Regenerative Medicine Research Center, and Department of Pharmacology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran
- Department of Orthopedics Surgery, Faculty of Medicine, Tissue Engineering Group (NOCERAL), University of Malaya, Kuala Lumpur, Malaysia
| | - Steven J. P. McInnes
- UniSA STEM, Mawson Lakes Campus, University of South Australia, Mawson Lakes, South Australia, Australia
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Dehkordi AN, Shafiei SS, Chehelgerdi M, Sabouni F, Sharifi E, Makvandi P, Nasrollahi N. Highly effective electrospun polycaprolactone/ layered double hydroxide nanofibrous scaffold for bone tissue engineering. J Drug Deliv Sci Technol 2022. [DOI: 10.1016/j.jddst.2022.103827] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 11/29/2022]
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Zhou J, Nie Y, Jin C, Zhang JXJ. Engineering Biomimetic Extracellular Matrix with Silica Nanofibers: From 1D Material to 3D Network. ACS Biomater Sci Eng 2022; 8:2258-2280. [PMID: 35377596 DOI: 10.1021/acsbiomaterials.1c01525] [Citation(s) in RCA: 3] [Impact Index Per Article: 1.0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 12/19/2022]
Abstract
Biomaterials at nanoscale is a fast-expanding research field with which extensive studies have been conducted on understanding the interactions between cells and their surrounding microenvironments as well as intracellular communications. Among many kinds of nanoscale biomaterials, mesoporous fibrous structures are especially attractive as a promising approach to mimic the natural extracellular matrix (ECM) for cell and tissue research. Silica is a well-studied biocompatible, natural inorganic material that can be synthesized as morpho-genetically active scaffolds by various methods. This review compares silica nanofibers (SNFs) to other ECM materials such as hydrogel, polymers, and decellularized natural ECM, summarizes fabrication techniques for SNFs, and discusses different strategies of constructing ECM using SNFs. In addition, the latest progress on SNFs synthesis and biomimetic ECM substrates fabrication is summarized and highlighted. Lastly, we look at the wide use of SNF-based ECM scaffolds in biological applications, including stem cell regulation, tissue engineering, drug release, and environmental applications.
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Affiliation(s)
- Junhu Zhou
- Thayer School of Engineering, Dartmouth College, Hanover, New Hampshire 03755, United States
| | - Yuan Nie
- Thayer School of Engineering, Dartmouth College, Hanover, New Hampshire 03755, United States
| | - Congran Jin
- Thayer School of Engineering, Dartmouth College, Hanover, New Hampshire 03755, United States
| | - John X J Zhang
- Thayer School of Engineering, Dartmouth College, Hanover, New Hampshire 03755, United States
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Effects of Nanofiber Scaffolds Coated with Nanoparticulate and Microparticulate Freeze Dried Bone Allograft on the Morphology, Adhesion, and Proliferation of Human Mesenchymal Stem Cells. IRANIAN BIOMEDICAL JOURNAL 2022; 26:193-201. [PMID: 35633638 PMCID: PMC9440688 DOI: 10.52547/ibj.26.3.193] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Track Full Text] [Download PDF] [Figures] [Subscribe] [Scholar Register] [Indexed: 11/23/2022]
Abstract
Background: Freeze dried bone allograft nanoparticles on a nanofiber membrane may serve as an ideal scaffold for bone regeneration. This study aimed to assess the biological behavior of human MSCs in terms of proliferation and adhesion to nanoparticulate and microparticulate FDBA scaffolds on PLLA nanofiber membrane. Methods: In this experimental study, PLLA nanofiber scaffolds were synthesized by the electrospinning method. The FDBA nanoparticles were synthesized mechanically. The FDBA nanoparticles and microparticles were loaded on the surface of PLLA nanofiber membrane. A total of 64 scaffold samples in four groups of n-FDBA/PLLA, FDBA/PLLA, PLLA and control were placed in 24-well polystyrene tissue culture plates; 16 wells were allocated to each group. Data were analyzed using one-way ANOVA and Bonferroni test. Results: The proliferation rate of MSCs was significantly higher in the nanoparticulate group compared to the microparticulate group at five days (p = 0.034). Assessment of cell morphology at 24 hours revealed spindle-shaped cells with a higher number of appendages in the nanoparticulate group compared to other groups. Conclusion: MSCs on n-FDBA/PLLA scaffold were morphologically more active and flatter with a higher number of cellular appendages, as compared to FDBA/PLLA. It seems that the nanoparticulate scaffold is superior to the microparticulate scaffold in terms of proliferation, attachment, and morphology of MSCs in vitro.
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Gama e Silva GL, Sato de Souza Bustamante Monteiro M, dos Santos Matos AP, Santos-Oliveira R, Kenechukwu FC, Ricci-Júnior E. Nanofibers in the treatment of osteomyelitis and bone regeneration. J Drug Deliv Sci Technol 2022. [DOI: 10.1016/j.jddst.2021.102999] [Citation(s) in RCA: 1] [Impact Index Per Article: 0.3] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 02/07/2023]
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11
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Sarangi D, Pattanaik S. Nanoparticles in dentistry. ADVANCED NANOMATERIALS FOR POINT OF CARE DIAGNOSIS AND THERAPY 2022:335-358. [DOI: 10.1016/b978-0-323-85725-3.00008-8] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Track Full Text] [Subscribe] [Scholar Register] [Indexed: 01/05/2025]
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12
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Lu Z, Wang W, Zhang J, Bártolo P, Gong H, Li J. Electrospun highly porous poly(L-lactic acid)-dopamine-SiO2 fibrous membrane for bone regeneration. MATERIALS SCIENCE & ENGINEERING. C, MATERIALS FOR BIOLOGICAL APPLICATIONS 2020; 117:111359. [DOI: 10.1016/j.msec.2020.111359] [Citation(s) in RCA: 18] [Impact Index Per Article: 3.6] [Reference Citation Analysis] [Track Full Text] [Subscribe] [Scholar Register] [Received: 05/29/2020] [Revised: 07/15/2020] [Accepted: 08/04/2020] [Indexed: 02/08/2023]
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13
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Shi Z, Xu Y, Mulatibieke R, Zhong Q, Pan X, Chen Y, Lian Q, Luo X, Shi Z, Zhu Q. Nano-Silicate-Reinforced and SDF-1α-Loaded Gelatin-Methacryloyl Hydrogel for Bone Tissue Engineering. Int J Nanomedicine 2020; 15:9337-9353. [PMID: 33262591 PMCID: PMC7699450 DOI: 10.2147/ijn.s270681] [Citation(s) in RCA: 14] [Impact Index Per Article: 2.8] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 07/09/2020] [Accepted: 11/02/2020] [Indexed: 12/19/2022] Open
Abstract
PURPOSE Autologous bone grafts are the gold standard for treating bone defects. However, limited bone supply and morbidity at the donor site restrict its extensive use. Therefore, developing bone graft materials as an alternative to autologous grafts has gained considerable attention. Injectable hydrogels endowed with osteogenic potential have the ability to fill irregular bone defects using minimally invasive procedures and have thus been attracting researchers' attention. However, from a clinical perspective, most fabrication methods employed for the current injectable osteogenic hydrogels are difficult and inconvenient. In the current study, we fabricated an injectable osteogenic hydrogel using a simple and convenient strategy. MATERIALS AND METHODS Gelatin-methacryloyl (GelMA) pre-polymer was synthetized. Nano silicate (SN) and stromal cell-derived factor-1 alpha (SDF-1α) were introduced into the pre-polymer to achieve injectability, controlled release property, excellent osteogenic ability, and efficient stem cell homing. RESULTS The GelMA-SN-SDF-1α demonstrated excellent injectability via a 17-G needle at room temperature. The loaded SDF-1α exhibited a long-term controlled release pattern and efficiently stimulated MSC migration and homing. The GelMA-SN-SDF-1α hydrogel amplified cell spreading, migration, osteogenic-related biomarker expression, and matrix mineralization. The GelMA-SN-SDF-1α hydrogel filled critical-sized calvaria defects in rats and demonstrated excellent bone regeneration ability, as assessed using micro-CT scanning and histomorphometric staining. CONCLUSION The GelMA-SN-SDF-1α hydrogel provides a simple and convenient strategy for the fabrication of injectable osteogenic graft materials.
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Affiliation(s)
- Zhe Shi
- Department of Orthopedics, Nanfang Hospital, Southern Medical University, Guangzhou, People’s Republic of China
| | - Yichuan Xu
- Department of Orthopedics, Nanfang Hospital, Southern Medical University, Guangzhou, People’s Republic of China
| | - Ruzha Mulatibieke
- Department of Plastic Surgery, The Third Affiliated Hospital of Sun Yet-Sen University, Guangzhou, People’s Republic of China
| | - Qiang Zhong
- Department of Orthopedics, Nanfang Hospital, Southern Medical University, Guangzhou, People’s Republic of China
| | - Xin Pan
- Department of Orthopedics, Nanfang Hospital, Southern Medical University, Guangzhou, People’s Republic of China
| | - Yuhang Chen
- Department of Orthopedics, Nanfang Hospital, Southern Medical University, Guangzhou, People’s Republic of China
| | - Qiang Lian
- Department of Orthopedics, Nanfang Hospital, Southern Medical University, Guangzhou, People’s Republic of China
| | - Xin Luo
- Rehabilitation Medical School, Guangzhou International Economics College, Guangzhou, People’s Republic of China
| | - Zhanjun Shi
- Department of Orthopedics, Nanfang Hospital, Southern Medical University, Guangzhou, People’s Republic of China
| | - Qingan Zhu
- Department of Orthopedics, Nanfang Hospital, Southern Medical University, Guangzhou, People’s Republic of China
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Development of novel 3D scaffolds using BioExtruder by varying the content of hydroxyapatite and silica in PCL matrix for bone tissue engineering. JOURNAL OF POLYMER RESEARCH 2020. [DOI: 10.1007/s10965-020-02053-0] [Citation(s) in RCA: 5] [Impact Index Per Article: 1.0] [Reference Citation Analysis] [Track Full Text] [Subscribe] [Scholar Register] [Indexed: 10/24/2022]
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15
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Homocianu M, Pascariu P. Electrospun Polymer-Inorganic Nanostructured Materials and Their Applications. POLYM REV 2019. [DOI: 10.1080/15583724.2019.1676776] [Citation(s) in RCA: 7] [Impact Index Per Article: 1.2] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 02/06/2023]
Affiliation(s)
| | - Petronela Pascariu
- “Petru Poni” Institute of Macromolecular Chemistry, Iasi, Romania
- Faculty of Electrical Engineering and Computer Science & MANSiD Research Center, Stefan cel Mare University, Suceava, Romania
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16
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Gandhimathi C, Quek YJ, Ezhilarasu H, Ramakrishna S, Bay BH, Srinivasan DK. Osteogenic Differentiation of Mesenchymal Stem Cells with Silica-Coated Gold Nanoparticles for Bone Tissue Engineering. Int J Mol Sci 2019; 20:E5135. [PMID: 31623264 PMCID: PMC6834165 DOI: 10.3390/ijms20205135] [Citation(s) in RCA: 28] [Impact Index Per Article: 4.7] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Grants] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 09/10/2019] [Revised: 10/14/2019] [Accepted: 10/14/2019] [Indexed: 01/23/2023] Open
Abstract
Multifunctional nanofibrous scaffolds for effective bone tissue engineering (BTE) application must incorporate factors to promote neovascularization and tissue regeneration. In this study, silica-coated gold nanoparticles Au(SiO2) were tested for their ability to promote differentiation of human mesenchymal stem cells (hMSCs) into osteoblasts. Biocompatible poly-ε-caprolactone (PCL), PCL/silk fibroin (SF) and PCL/SF/Au(SiO2) loaded nanofibrous scaffolds were first fabricated by an electrospinning method. Electrospun nanofibrous scaffolds were characterized for fiber architecture, porosity, pore size distribution, fiber wettability and the relevant mechanical properties using field emission scanning electron microscopy (FESEM), porosimetry, determination of water contact angle, measurements by a surface analyzer and tabletop tensile-tester measurements. FESEM images of the scaffolds revealed beadless, porous, uniform fibers with diameters in the range of 164 ± 18.65 nm to 215 ± 32.12 nm and porosity of around 88-92% and pore size distribution around 1.45-2.35 µm. Following hMSCs were cultured on the composite scaffolds. Cell-scaffold interaction, morphology and proliferation of were analyzed by FESEM analysis, MTS (3-(4,5-dimethyl thiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium inner salt) and CMFDA (5-choromethyl fluorescein acetate) dye assays. Osteogenic differentiation of MSCs into osteogenic cells were determined by alkaline phosphatase (ALP) activity, mineralization by alizarin red S (ARS) staining and osteocalcin expression by immunofluorescence staining. The results revealed that the addition of SF and Au(SiO2) to PCL scaffolds enhanced the mechanical strength, interconnecting porous structure and surface roughness of the scaffolds. This, in turn, led to successful osteogenic differentiation of hMSCs with improved cell adhesion, proliferation, differentiation, mineralization and expression of pro-osteogenic cellular proteins. This provides huge support for Au(SiO2) as a suitable material in BTE.
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Affiliation(s)
- Chinnasamy Gandhimathi
- Temasek Life Sciences Laboratory, National University of Singapore, Singapore 117604, Singapore.
- Lee Kong Chian School of Medicine, Nanyang Technological University, Singapore 636921, Singapore.
| | - Ying Jie Quek
- Lee Kong Chian School of Medicine, Nanyang Technological University, Singapore 636921, Singapore.
- School of Biological Sciences, Nanyang Technological University, Singapore 637551, Singapore.
| | - Hariharan Ezhilarasu
- Department of Anatomy, Yong Loo Lin School of Medicine, National University of Singapore, Singapore 117594, Singapore.
| | - Seeram Ramakrishna
- Department of Mechanical Engineering, Center for Nanofibers & Nanotechnology, Faculty of Engineering, National University of Singapore, Singapore 117576, Singapore.
| | - Boon-Huat Bay
- Department of Anatomy, Yong Loo Lin School of Medicine, National University of Singapore, Singapore 117594, Singapore.
| | - Dinesh Kumar Srinivasan
- Lee Kong Chian School of Medicine, Nanyang Technological University, Singapore 636921, Singapore.
- Department of Anatomy, Yong Loo Lin School of Medicine, National University of Singapore, Singapore 117594, Singapore.
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17
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Hokmabad VR, Davaran S, Aghazadeh M, Rahbarghazi R, Salehi R, Ramazani A. Fabrication and characterization of novel ethyl cellulose-grafted-poly (ɛ-caprolactone)/alginate nanofibrous/macroporous scaffolds incorporated with nano-hydroxyapatite for bone tissue engineering. J Biomater Appl 2019; 33:1128-1144. [PMID: 30651055 DOI: 10.1177/0885328218822641] [Citation(s) in RCA: 29] [Impact Index Per Article: 4.8] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 01/30/2023]
Abstract
The major challenge of tissue regeneration is to develop three dimensional scaffolds with suitable properties which would mimic the natural extracellular matrix to induce the adhesion, proliferation, and differentiation of cells. Several materials have been used for the preparation of the scaffolds for bone regeneration. In this study, novel ethyl cellulose-grafted-poly (ɛ-caprolactone) (EC-g-PCL)/alginate scaffolds with different contents of nano-hydroxyapatite were prepared by combining electrospinning and freeze-drying methods in order to provide nanofibrous/macroporous structures with good mechanical properties. For this aim, EC-g-PCL nanofibers were obtained with electrospinning, embedded layer-by-layer in alginate solutions containing nano-hydroxyapatite particles, and finally, these constructions were freeze-dried. The scaffolds possess highly porous structures with interconnected pore network. The swelling, porosity, and degradation characteristics of the EC-g-PCL/alginate scaffolds were decreased with the increase in nano-hydroxyapatite contents, whereas increases in the in-vitro biomineralization and mechanical strength were observed as the nano-hydroxyapatite content was increased. The cell response to EC-g-PCL/alginate scaffolds with/or without nano-hydroxyapatite was investigated using human dental pulp stem cells (hDPSCs). hDPSCs displayed a high adhesion, proliferation, and differentiation on nano-hydroxyapatite-incorporated EC-g-PCL/alginate scaffolds compared to pristine EC-g-PCL/alginate scaffold. Overall, these results suggested that the EC-g-PCL/alginate-HA scaffolds might have potential applications in bone tissue engineering.
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Affiliation(s)
- Vahideh Raeisdasteh Hokmabad
- 1 Drug Applied Research Center and Department of Medical Nanotechnology, Faculty of Advanced Medical Science, Tabriz University of Medical Sciences, Tabriz, Iran.,2 Department of Chemistry, University of Zanjan, Zanjan, Iran
| | - Soodabeh Davaran
- 1 Drug Applied Research Center and Department of Medical Nanotechnology, Faculty of Advanced Medical Science, Tabriz University of Medical Sciences, Tabriz, Iran
| | - Marziyeh Aghazadeh
- 3 Stem Cell Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.,4 Oral Medicine Department of Dental Faculty, Tabriz University of Medical Sciences, Tabriz, Iran
| | - Reza Rahbarghazi
- 3 Stem Cell Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.,5 Department of Applied Cell Sciences, Faculty of Advanced Medical Science, Tabriz University of Medical Sciences, Tabriz, Iran
| | - Roya Salehi
- 1 Drug Applied Research Center and Department of Medical Nanotechnology, Faculty of Advanced Medical Science, Tabriz University of Medical Sciences, Tabriz, Iran
| | - Ali Ramazani
- 2 Department of Chemistry, University of Zanjan, Zanjan, Iran
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18
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Burton CW, DiFeo Childs R, McClellan P, Yu Q, Bundy J, Gao M, Evans E, Landis W. Silica/polycaprolactone nanofiber scaffold variants for human periosteal cell growth. J Biomed Mater Res A 2019; 107:791-801. [DOI: 10.1002/jbm.a.36595] [Citation(s) in RCA: 3] [Impact Index Per Article: 0.5] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 08/10/2018] [Revised: 12/01/2018] [Accepted: 12/12/2018] [Indexed: 01/01/2023]
Affiliation(s)
| | | | - Phillip McClellan
- University of Akron; Akron Ohio
- Case Western Reserve University; Cleveland Ohio
| | - Qing Yu
- University of Akron; Akron Ohio
| | - Joshua Bundy
- University of Akron; Akron Ohio
- Indiana University; Bloomington Indiana
| | - Min Gao
- Kent State University; Kent Ohio
| | | | - William Landis
- University of Akron; Akron Ohio
- University of California, San Francisco; San Francisco California
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19
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Bakht Khosh Hagh H, Farshi Azhar F. Reinforcing materials for polymeric tissue engineering scaffolds: A review. J Biomed Mater Res B Appl Biomater 2018; 107:1560-1575. [DOI: 10.1002/jbm.b.34248] [Citation(s) in RCA: 13] [Impact Index Per Article: 1.9] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 02/11/2018] [Revised: 08/11/2018] [Accepted: 08/31/2018] [Indexed: 12/12/2022]
Affiliation(s)
- Haleh Bakht Khosh Hagh
- Polymer Composite Research Laboratory, Department of Applied ChemistryFaculty of Chemistry, University of Tabriz Tabriz 5166614766 Iran
| | - Fahimeh Farshi Azhar
- Applied Chemistry Research Laboratory, Department of ChemistryFaculty of Sciences, Azarbaijan Shahid Madani University Tabriz 5375171379 Iran
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20
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Wang S, Hu F, Li J, Zhang S, Shen M, Huang M, Shi X. Design of electrospun nanofibrous mats for osteogenic differentiation of mesenchymal stem cells. NANOMEDICINE : NANOTECHNOLOGY, BIOLOGY, AND MEDICINE 2018; 14:2505-2520. [PMID: 28554595 DOI: 10.1016/j.nano.2016.12.024] [Citation(s) in RCA: 53] [Impact Index Per Article: 7.6] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Subscribe] [Scholar Register] [Received: 09/22/2016] [Revised: 12/20/2016] [Accepted: 12/30/2016] [Indexed: 01/09/2023]
Abstract
The clinical translation potential of mesenchymal stem cells (MSCs) in regenerative medicine has been greatly exploited. With the merits of high surface area to volume ratio, facile control of components, well retained topography, and the capacity to mimic the native extracellular matrix (ECM), nanofibers have received a great deal of attention as bone tissue engineering scaffolds. Electrospinning has been considered as an efficient approach for scale-up fabrication of nanofibrous materials. Electrospun nanofibers are capable of stimulating cell-matrix interaction to form a cell niche, directing cellular behavior, and promoting the MSCs adhesion and proliferation. In this review, we give a comprehensive literature survey on the mechanisms of electrospun nanofibers in supporting the MSCs differentiation. Specifically, the influences of biological and physical osteogenic inductive cues on the MSCs osteogenic differentiation are reviewed. Along with the significant advances in the field, current research challenges and future perspectives are also discussed.
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Affiliation(s)
- Shige Wang
- College of Science, University of Shanghai for Science & Technology, Shanghai, PR China; State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai, PR China; State Key Laboratory of Molecular Engineering of Polymers, Fudan University, Shanghai, People's Republic of China
| | - Fei Hu
- College of Science, University of Shanghai for Science & Technology, Shanghai, PR China
| | - Jingchao Li
- State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai, PR China
| | - Shuping Zhang
- College of Science, University of Shanghai for Science & Technology, Shanghai, PR China
| | - Mingwu Shen
- State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai, PR China
| | - Mingxian Huang
- College of Science, University of Shanghai for Science & Technology, Shanghai, PR China.
| | - Xiangyang Shi
- State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai, PR China; CQM-Centro de Química da Madeira, Universidade da Madeira, Campus da Penteada, Funchal, Portugal.
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21
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Hokmabad VR, Davaran S, Aghazadeh M, Alizadeh E, Salehi R, Ramazani A. A Comparison of the Effects of Silica and Hydroxyapatite Nanoparticles on Poly(ε-caprolactone)-Poly(ethylene glycol)-Poly(ε-caprolactone)/Chitosan Nanofibrous Scaffolds for Bone Tissue Engineering. Tissue Eng Regen Med 2018; 15:735-750. [PMID: 30603592 DOI: 10.1007/s13770-018-0140-z] [Citation(s) in RCA: 56] [Impact Index Per Article: 8.0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 02/15/2018] [Revised: 06/26/2018] [Accepted: 06/27/2018] [Indexed: 11/29/2022] Open
Abstract
Background The major challenge of tissue engineering is to develop constructions with suitable properties which would mimic the natural extracellular matrix to induce the proliferation and differentiation of cells. Poly(ɛ-caprolactone)-poly(ethylene glycol)-poly(ɛ-caprolactone) (PCL-PEG-PCL, PCEC), chitosan (CS), nano-silica (n-SiO2) and nano-hydroxyapatite (n-HA) are biomaterials successfully applied for the preparation of 3D structures appropriate for tissue engineering. Methods We evaluated the effect of n-HA and n-SiO2 incorporated PCEC-CS nanofibers on physical properties and osteogenic differentiation of human dental pulp stem cells (hDPSCs). Fourier transform infrared spectroscopy, field emission scanning electron microscope, transmission electron microscope, thermogravimetric analysis, contact angle and mechanical test were applied to evaluate the physicochemical properties of nanofibers. Cell adhesion and proliferation of hDPSCs and their osteoblastic differentiation on nanofibers were assessed using MTT assay, DAPI staining, alizarin red S staining, and QRT-PCR assay. Results All the samples demonstrated bead-less morphologies with an average diameter in the range of 190-260 nm. The mechanical test studies showed that scaffolds incorporated with n-HA had a higher tensile strength than ones incorporated with n-SiO2. While the hydrophilicity of n-SiO2 incorporated PCEC-CS nanofibers was higher than that of samples enriched with n-HA. Cell adhesion and proliferation studies showed that n-HA incorporated nanofibers were slightly superior to n-SiO2 incorporated ones. Alizarin red S staining and QRT-PCR analysis confirmed the osteogenic differentiation of hDPSCs on PCEC-CS nanofibers incorporated with n-HA and n-SiO2. Conclusion Compared to other groups, PCEC-CS nanofibers incorporated with 15 wt% n-HA were able to support more cell adhesion and differentiation, thus are better candidates for bone tissue engineering applications.
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Affiliation(s)
| | - Soodabeh Davaran
- 2Drug Applied Research Center and Department of Medical Nanotechnology, Faculty of Advanced Medical Science, Tabriz University of Medical Sciences, Tabriz, 51666-14733 Iran
| | - Marziyeh Aghazadeh
- 3Stem Cell Research Center, Tabriz University of Medical Sciences, Tabriz, 51666-14733 Iran.,4Oral Medicine Department of Dental Faculty, Tabriz University of Medical Sciences, Tabriz, 51666-14733 Iran
| | - Effat Alizadeh
- 3Stem Cell Research Center, Tabriz University of Medical Sciences, Tabriz, 51666-14733 Iran.,5Department of Medical Biotechnology, Faculty of Advanced Medical Science, Tabriz University of Medical Sciences, Tabriz, 51666-14733 Iran
| | - Roya Salehi
- 2Drug Applied Research Center and Department of Medical Nanotechnology, Faculty of Advanced Medical Science, Tabriz University of Medical Sciences, Tabriz, 51666-14733 Iran
| | - Ali Ramazani
- 1Department of Chemistry, University of Zanjan, P.O. Box 45195-313, Zanjan, Iran
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22
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Porous silicon-poly(ε-caprolactone) film composites: evaluation of drug release and degradation behavior. Biomed Microdevices 2018; 20:71. [PMID: 30097808 DOI: 10.1007/s10544-018-0313-5] [Citation(s) in RCA: 1] [Impact Index Per Article: 0.1] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 10/28/2022]
Abstract
This work focuses on an evaluation of novel composites of porous silicon (pSi) with the biocompatible polymer ε-polycaprolactone (PCL) for drug delivery and tissue engineering applications. The degradation behavior of the composites in terms of their morphology along with the effect of pSi on polymer degradation was monitored. PSi particles loaded with the drug camptothecin (CPT) were physically embedded into PCL films formed from electrospun PCL fiber sheets. PSi/PCL composites revealed a release profile of CPT (monitored via fluorescence spectroscopy) in accordance with the Higuchi release model, with significantly lower burst release percentage compared to pSi microparticles alone. Degradation studies of the composites, using gravimetric analysis, differential scanning calorimetry (DSC), and field emission scanning electron microscopy (FESEM), carried out in phosphate-buffered saline (PBS) under simulated physiological conditions, indicated a modest mass loss (15%) over 5 weeks due to pSi dissolution and minor polymer hydrolysis. DSC results showed that, relative to PCL-only controls, pSi suppressed crystallization of the polymer film during PBS exposure. This suppression affects the evolution of surface morphology during this exposure that, in turn, influences the degradation behavior of the polymer. The implications of the above properties of these composites as a possible therapeutic device are discussed.
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23
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Castro AG, Diba M, Kersten M, Jansen JA, van den Beucken JJ, Yang F. Development of a PCL-silica nanoparticles composite membrane for Guided Bone Regeneration. MATERIALS SCIENCE & ENGINEERING. C, MATERIALS FOR BIOLOGICAL APPLICATIONS 2018; 85:154-161. [DOI: 10.1016/j.msec.2017.12.023] [Citation(s) in RCA: 62] [Impact Index Per Article: 8.9] [Reference Citation Analysis] [Track Full Text] [Subscribe] [Scholar Register] [Received: 06/29/2017] [Revised: 08/27/2017] [Accepted: 12/19/2017] [Indexed: 12/19/2022]
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24
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Kuttappan S, Anitha A, Minsha MG, Menon PM, Sivanarayanan TB, Vijayachandran LS, Nair MB. BMP2 expressing genetically engineered mesenchymal stem cells on composite fibrous scaffolds for enhanced bone regeneration in segmental defects. MATERIALS SCIENCE & ENGINEERING. C, MATERIALS FOR BIOLOGICAL APPLICATIONS 2018; 85:239-248. [PMID: 29407153 DOI: 10.1016/j.msec.2018.01.001] [Citation(s) in RCA: 14] [Impact Index Per Article: 2.0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Subscribe] [Scholar Register] [Received: 08/21/2017] [Revised: 11/23/2017] [Accepted: 01/09/2018] [Indexed: 12/21/2022]
Abstract
The treatment of critical sized bone defect remains a significant challenge in orthopedics. The objective of the study is to evaluate the effect of the combination of bone morphogenetic protein 2 (BMP2) expressing genetically engineered mesenchymal stem cells (MSCs) [MSCs engineered using a multimam vector, pAceMam1, an emerging gene delivery vector] and an osteoconductive scaffold [silica coated nanohydroxyapatite-gelatin reinforced with fibers] in enhancing bone regeneration in critical sized segmental defects. The scaffold with transfected MSCs showed significantly higher viability, proliferation and osteogenic differentiation in vitro. Further, this group augmented union and new bone formation in critical sized rat femoral segmental defect at 12 weeks when compared to control groups (scaffold with MSCs and scaffold alone). These data demonstrated that the MSCs engineered for transient expression of BMP2 can improve the repair of segmental defects, which paves an avenue for using pAceMam1 as a vector for bone tissue regeneration.
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Affiliation(s)
- Shruthy Kuttappan
- Amrita Center for Nanosciences and Molecular Medicine, Amrita Institute of Medical Sciences and Research Center, Amrita Vishwa Vidyapeetham, Kochi, Kerala 682041, India
| | - A Anitha
- Amrita Center for Nanosciences and Molecular Medicine, Amrita Institute of Medical Sciences and Research Center, Amrita Vishwa Vidyapeetham, Kochi, Kerala 682041, India
| | - M G Minsha
- Amrita Center for Nanosciences and Molecular Medicine, Amrita Institute of Medical Sciences and Research Center, Amrita Vishwa Vidyapeetham, Kochi, Kerala 682041, India
| | - Parvathy M Menon
- Amrita Center for Nanosciences and Molecular Medicine, Amrita Institute of Medical Sciences and Research Center, Amrita Vishwa Vidyapeetham, Kochi, Kerala 682041, India
| | - T B Sivanarayanan
- Central Animal Lab Facility, Amrita Institute of Medical Sciences and Research Center, Amrita Vishwa Vidyapeetham, Kochi, Kerala 682041, India
| | - Lakshmi Sumitra Vijayachandran
- Amrita Center for Nanosciences and Molecular Medicine, Amrita Institute of Medical Sciences and Research Center, Amrita Vishwa Vidyapeetham, Kochi, Kerala 682041, India.
| | - Manitha B Nair
- Amrita Center for Nanosciences and Molecular Medicine, Amrita Institute of Medical Sciences and Research Center, Amrita Vishwa Vidyapeetham, Kochi, Kerala 682041, India.
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25
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Navarro-Suarez S, Flores-Palma A, Flores-Ruiz R, Gutiérrez-Pérez JL, Torres-Lagares D. Nanobiomaterials in dentistry. NANOBIOMATERIALS 2018:297-318. [DOI: 10.1016/b978-0-08-100716-7.00011-8] [Citation(s) in RCA: 2] [Impact Index Per Article: 0.3] [Reference Citation Analysis] [Track Full Text] [Subscribe] [Scholar Register] [Indexed: 01/05/2025]
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26
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Suryavanshi A, Khanna K, Sindhu KR, Bellare J, Srivastava R. Magnesium oxide nanoparticle-loaded polycaprolactone composite electrospun fiber scaffolds for bone-soft tissue engineering applications: in-vitro and in-vivo evaluation. ACTA ACUST UNITED AC 2017; 12:055011. [PMID: 28944766 DOI: 10.1088/1748-605x/aa792b] [Citation(s) in RCA: 52] [Impact Index Per Article: 6.5] [Reference Citation Analysis] [Abstract] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 11/11/2022]
Abstract
The objective of the present investigation was to assess the potential of magnesium oxide nanoparticle (MgO NP)-loaded electrospun polycaprolactone (PCL) polymer composites as a bone-soft tissue engineering scaffold. MgO NPs were synthesized using a hydroxide precipitation sol-gel method and characterized using field emission gun-scanning electron microscopy/energy-dispersive x-ray spectroscopy (FEG-SEM/EDS), field emission gun-transmission electron microscopy (FEG-TEM), and x-ray diffraction (XRD) analysis. PCL and MgO-PCL nanocomposite fibers were fabricated using electrospinning with trifluoroethanol as solvent at 19 kV applied voltage and 1.9 ml h-1 flow rate as optimized process parameters, and were characterized by FEG-TEM, FEG-SEM/EDS, XRD, and differential scanning calorimetry analyses. Characterization studies of as-synthesized nanoparticles revealed diffraction peaks indexed to various crystalline planes peculiar to MgO particles with hexagonal and cubical shape, and 40-60 nm size range. Significant improvement in mechanical properties (tensile strength and elastic modulus) of nanocomposites was observed as compared to neat polymer specimens (fourfold and threefold, respectively), due to uniform dispersion of nanofillers along the polymer fiber length. There was a remarkable bioactivity shown by nanocomposite scaffolds in immersion test, as indicated by formation of surface hydroxyapatite layer by the third day of incubation. MgO-loaded electrospun PCL mats showed enhanced in-vitro biological performance with osteoblast-like MG-63 cells in terms of adhesion, proliferation, and marked differentiation marker activity owing to greater surface roughness, nanotopography, and hydrophilicity facilitating higher protein adsorption. In-vivo subcutaneous implantation study in Sprague Dawley rats revealed initial moderate inflammatory tissue response near implant site at the second week timepoint that subsided later (eighth week) with no adverse effect on vital organ functionalities as seen in histopathological analysis supported by serum biochemical and hematological parameters which did not deviate significantly from normal physiological range, indicating good biocompatibility in-vivo. Thus, MgO-PCL nanocomposite electrospun fibers have potential as an efficient scaffold material for bone-soft tissue engineering applications.
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Affiliation(s)
- Ajay Suryavanshi
- NanoBios Laboratory, Department of Biosciences and Bioengineering, Indian Institute of Technology Bombay, Mumbai, India
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27
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Samanta A, Takkar S, Kulshreshtha R, Nandan B, Srivastava RK. Facile Fabrication of Composite Electrospun Nanofibrous Matrices of Poly(ε-caprolactone)-Silica Based Pickering Emulsion. LANGMUIR : THE ACS JOURNAL OF SURFACES AND COLLOIDS 2017; 33:8062-8069. [PMID: 28735544 DOI: 10.1021/acs.langmuir.7b02119] [Citation(s) in RCA: 6] [Impact Index Per Article: 0.8] [Reference Citation Analysis] [Abstract] [Track Full Text] [Subscribe] [Scholar Register] [Indexed: 06/07/2023]
Abstract
Functionalized matrices have been sought for their application in sensors, filtration, energy storage, catalysis, and tissue engineering. We report formation of an inorganic-organic composite matrix based on poly(ε-caprolactone) (PCL) functionalized with hydrophobically modified silica (m-silica) fabricated with reduced organic solvent usage. The matrix was obtained via electrospinning of a water-in-oil emulsion of PCL that was stabilized by judicious choice of m-silica as a Pickering agent resulting into an emulsifier free matrix. Inclusion of m-silica in PCL matrix resulted in enhancing tensile properties and cell proliferation efficiency. The electrospun composite matrix was free from any emulsifier or template polymer; thus any abrupt loss in mechanical properties was prevented when the matrix was subjected to aqueous conditions. The inorganic-organic biodegradable composite matrices thus produced using an emulsifier free emulsion find applications in tissue engineering and may further be evaluated for other areas including selective sorption and separation.
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Affiliation(s)
- Archana Samanta
- Department of Textile Technology, Indian Institute of Technology Delhi , Hauz Khas, New Delhi 110016, India
| | - Sonam Takkar
- Department of Biochemical Engineering and Biotechnology, Indian Institute of Technology Delhi , Hauz Khas, New Delhi 110016, India
| | - Ritu Kulshreshtha
- Department of Biochemical Engineering and Biotechnology, Indian Institute of Technology Delhi , Hauz Khas, New Delhi 110016, India
| | - Bhanu Nandan
- Department of Textile Technology, Indian Institute of Technology Delhi , Hauz Khas, New Delhi 110016, India
| | - Rajiv K Srivastava
- Department of Textile Technology, Indian Institute of Technology Delhi , Hauz Khas, New Delhi 110016, India
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28
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Chen X, Gleeson SE, Yu T, Khan N, Yucha RW, Marcolongo M, Li CY. Hierarchically ordered polymer nanofiber shish kebabs as a bone scaffold material. J Biomed Mater Res A 2017; 105:1786-1798. [DOI: 10.1002/jbm.a.36039] [Citation(s) in RCA: 24] [Impact Index Per Article: 3.0] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 11/28/2016] [Revised: 02/08/2017] [Accepted: 02/10/2017] [Indexed: 01/23/2023]
Affiliation(s)
- Xi Chen
- Department of Materials Science and EngineeringDrexel UniversityPhiladelphia Pennsylvania19104
| | - Sarah E. Gleeson
- Department of Materials Science and EngineeringDrexel UniversityPhiladelphia Pennsylvania19104
| | - Tony Yu
- Department of Materials Science and EngineeringDrexel UniversityPhiladelphia Pennsylvania19104
| | - Nasreen Khan
- Department of Materials Science and EngineeringDrexel UniversityPhiladelphia Pennsylvania19104
| | - Robert W. Yucha
- Department of Materials Science and EngineeringDrexel UniversityPhiladelphia Pennsylvania19104
| | - Michele Marcolongo
- Department of Materials Science and EngineeringDrexel UniversityPhiladelphia Pennsylvania19104
| | - Christopher Y. Li
- Department of Materials Science and EngineeringDrexel UniversityPhiladelphia Pennsylvania19104
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29
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Cheng H, Yue K, Kazemzadeh-Narbat M, Liu Y, Khalilpour A, Li B, Zhang YS, Annabi N, Khademhosseini A. Mussel-Inspired Multifunctional Hydrogel Coating for Prevention of Infections and Enhanced Osteogenesis. ACS APPLIED MATERIALS & INTERFACES 2017; 9:11428-11439. [PMID: 28140564 PMCID: PMC5844698 DOI: 10.1021/acsami.6b16779] [Citation(s) in RCA: 156] [Impact Index Per Article: 19.5] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Grants] [Track Full Text] [Subscribe] [Scholar Register] [Indexed: 05/21/2023]
Abstract
Prevention of postsurgery infection and promotion of biointegration are the key factors to achieve long-term success in orthopedic implants. Localized delivery of antibiotics and bioactive molecules by the implant surface serves as a promising approach toward these goals. However, previously reported methods for surface functionalization of the titanium alloy implants to load bioactive ingredients suffer from time-consuming complex processes and lack of long-term stability. Here, we present the design and characterization of an adhesive, osteoconductive, and antimicrobial hydrogel coating for Ti implants. To form this multifunctional hydrogel, a photo-cross-linkable gelatin-based hydrogel was modified with catechol motifs to enhance adhesion to Ti surfaces and thus promote coating stability. To induce antimicrobial and osteoconductive properties, a short cationic antimicrobial peptide (AMP) and synthetic silicate nanoparticles (SNs) were introduced into the hydrogel formulation. The controlled release of AMP loaded in the hydrogel demonstrated excellent antimicrobial activity to prevent biofilm formation. Moreover, the addition of SNs to the hydrogel formulation enhanced osteogenesis when cultured with human mesenchymal stem cells, suggesting the potential to promote new bone formation in the surrounding tissues. Considering the unique features of our implant hydrogel coating, including high adhesion, antimicrobial capability, and the ability to induce osteogenesis, it is believed that our design provides a useful alternative method for bone implant surface modification and functionalization.
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Affiliation(s)
- Hao Cheng
- Biomaterials Innovation Research Center, Division of Biomedical Engineering, Department of Medicine, Brigham and Women’s Hospital, Harvard Medical School, Cambridge, Massachusetts 02139, United States
- Harvard–MIT Division of Health Sciences and Technology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, United States
- Othopeadic Department, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China
| | - Kan Yue
- Biomaterials Innovation Research Center, Division of Biomedical Engineering, Department of Medicine, Brigham and Women’s Hospital, Harvard Medical School, Cambridge, Massachusetts 02139, United States
- Harvard–MIT Division of Health Sciences and Technology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, United States
| | - Mehdi Kazemzadeh-Narbat
- Biomaterials Innovation Research Center, Division of Biomedical Engineering, Department of Medicine, Brigham and Women’s Hospital, Harvard Medical School, Cambridge, Massachusetts 02139, United States
- Harvard–MIT Division of Health Sciences and Technology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, United States
| | - Yanhui Liu
- Biomaterials Innovation Research Center, Division of Biomedical Engineering, Department of Medicine, Brigham and Women’s Hospital, Harvard Medical School, Cambridge, Massachusetts 02139, United States
- Harvard–MIT Division of Health Sciences and Technology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, United States
- College of Textiles, Donghua University, Shanghai, 201620, China
| | - Akbar Khalilpour
- Biomaterials Innovation Research Center, Division of Biomedical Engineering, Department of Medicine, Brigham and Women’s Hospital, Harvard Medical School, Cambridge, Massachusetts 02139, United States
- Harvard–MIT Division of Health Sciences and Technology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, United States
| | - Bingyun Li
- Department of Orthopaedics, School of Medicine, West Virginia University, Morgantown, West Virginia 26506, United States
| | - Yu Shrike Zhang
- Biomaterials Innovation Research Center, Division of Biomedical Engineering, Department of Medicine, Brigham and Women’s Hospital, Harvard Medical School, Cambridge, Massachusetts 02139, United States
- Harvard–MIT Division of Health Sciences and Technology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, United States
| | - Nasim Annabi
- Biomaterials Innovation Research Center, Division of Biomedical Engineering, Department of Medicine, Brigham and Women’s Hospital, Harvard Medical School, Cambridge, Massachusetts 02139, United States
- Harvard–MIT Division of Health Sciences and Technology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, United States
- Department of Chemical Engineering, Northeastern University, Boston, Massachusetts 02115-5000, United States
| | - Ali Khademhosseini
- Biomaterials Innovation Research Center, Division of Biomedical Engineering, Department of Medicine, Brigham and Women’s Hospital, Harvard Medical School, Cambridge, Massachusetts 02139, United States
- Harvard–MIT Division of Health Sciences and Technology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, United States
- Department of Bioindustrial Technologies, College of Animal Bioscience and Technology, Konkuk University, Seoul, 143-701, the Republic of Korea
- Department of Physics, King Abdulaziz University, Jeddah 21569, Saudi Arabia
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Cheng J, Jun Y, Qin J, Lee SH. Electrospinning versus microfluidic spinning of functional fibers for biomedical applications. Biomaterials 2017; 114:121-143. [DOI: 10.1016/j.biomaterials.2016.10.040] [Citation(s) in RCA: 190] [Impact Index Per Article: 23.8] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 07/08/2016] [Revised: 09/29/2016] [Accepted: 10/27/2016] [Indexed: 12/31/2022]
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Yi DK, Nanda SS, Kim K, Tamil Selvan S. Recent progress in nanotechnology for stem cell differentiation, labeling, tracking and therapy. J Mater Chem B 2017; 5:9429-9451. [DOI: 10.1039/c7tb02532g] [Citation(s) in RCA: 39] [Impact Index Per Article: 4.9] [Reference Citation Analysis] [Abstract] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 12/19/2022]
Abstract
Nanotechnology advancements for stem cell differentiation, labeling, tracking and therapeutic applications in cardiac repair, bone, and liver regeneration are delineated.
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Affiliation(s)
- Dong Kee Yi
- Department of Chemistry
- Myongji University
- Yongin 449-728
- South Korea
| | | | - Kwangmeyung Kim
- Center for Theragnosis
- Biomedical Research Institute
- Korea Institute of Science and Technology (KIST)
- Seoul
- South Korea
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Griffin MF, Kalaskar DM, Seifalian A, Butler PE. An update on the Application of Nanotechnology in Bone Tissue Engineering. Open Orthop J 2016; 10:836-848. [PMID: 28217209 PMCID: PMC5299580 DOI: 10.2174/1874325001610010836] [Citation(s) in RCA: 19] [Impact Index Per Article: 2.1] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Submit a Manuscript] [Subscribe] [Scholar Register] [Received: 01/27/2016] [Revised: 05/31/2016] [Accepted: 05/31/2016] [Indexed: 12/23/2022] Open
Abstract
Background: Natural bone is a complex and hierarchical structure. Bone possesses an extracellular matrix that has a precise nano-sized environment to encourage osteoblasts to lay down bone by directing them through physical and chemical cues. For bone tissue regeneration, it is crucial for the scaffolds to mimic the native bone structure. Nanomaterials, with features on the nanoscale have shown the ability to provide the appropriate matrix environment to guide cell adhesion, migration and differentiation. Methods: This review summarises the new developments in bone tissue engineering using nanobiomaterials. The design and selection of fabrication methods and biomaterial types for bone tissue engineering will be reviewed. The interactions of cells with different nanostructured scaffolds will be discussed including nanocomposites, nanofibres and nanoparticles. Results: Several composite nanomaterials have been able to mimic the architecture of natural bone. Bioceramics biomaterials have shown to be very useful biomaterials for bone tissue engineering as they have osteoconductive and osteoinductive properties. Nanofibrous scaffolds have the ability to provide the appropriate matrix environment as they can mimic the extracellular matrix structure of bone. Nanoparticles have been used to deliver bioactive molecules and label and track stem cells. Conclusion: Future studies to improve the application of nanomaterials for bone tissue engineering are needed.
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Affiliation(s)
- M F Griffin
- University College London, Centre for Nanotechnology & Regenerative Medicine, UCL Division of Surgery & Interventional Science, London, UK; Department of Plastic and Reconstructive Surgery, Royal Free Hampstead NHS Trust Hospital, London, UK
| | - D M Kalaskar
- University College London, Centre for Nanotechnology & Regenerative Medicine, UCL Division of Surgery & Interventional Science, London, UK; Department of Plastic and Reconstructive Surgery, Royal Free Hampstead NHS Trust Hospital, London, UK
| | - A Seifalian
- University College London, Centre for Nanotechnology & Regenerative Medicine, UCL Division of Surgery & Interventional Science, London, UK; Department of Plastic and Reconstructive Surgery, Royal Free Hampstead NHS Trust Hospital, London, UK
| | - P E Butler
- University College London, Centre for Nanotechnology & Regenerative Medicine, UCL Division of Surgery & Interventional Science, London, UK; Department of Plastic and Reconstructive Surgery, Royal Free Hampstead NHS Trust Hospital, London, UK
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Incorporation of mesoporous silica nanoparticles into random electrospun PLGA and PLGA/gelatin nanofibrous scaffolds enhances mechanical and cell proliferation properties. MATERIALS SCIENCE & ENGINEERING. C, MATERIALS FOR BIOLOGICAL APPLICATIONS 2016; 66:25-32. [DOI: 10.1016/j.msec.2016.04.031] [Citation(s) in RCA: 74] [Impact Index Per Article: 8.2] [Reference Citation Analysis] [Track Full Text] [Subscribe] [Scholar Register] [Received: 12/09/2015] [Revised: 03/18/2016] [Accepted: 04/11/2016] [Indexed: 01/19/2023]
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An overview of chitin or chitosan/nano ceramic composite scaffolds for bone tissue engineering. Int J Biol Macromol 2016; 93:1338-1353. [PMID: 27012892 DOI: 10.1016/j.ijbiomac.2016.03.041] [Citation(s) in RCA: 142] [Impact Index Per Article: 15.8] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 01/06/2016] [Revised: 03/03/2016] [Accepted: 03/20/2016] [Indexed: 01/06/2023]
Abstract
Chitin and chitosan based nanocomposite scaffolds have been widely used for bone tissue engineering. These chitin and chitosan based scaffolds were reinforced with nanocomponents viz Hydroxyapatite (HAp), Bioglass ceramic (BGC), Silicon dioxide (SiO2), Titanium dioxide (TiO2) and Zirconium oxide (ZrO2) to develop nanocomposite scaffolds. Plenty of works have been reported on the applications and characteristics of the nanoceramic composites however, compiling the work done in this field and presenting it in a single article is a thrust area. This review is written with an aim to fill this gap and focus on the preparations and applications of chitin or chitosan/nHAp, chitin or chitosan/nBGC, chitin or chitosan/nSiO2, chitin or chitosan/nTiO2 and chitin or chitosan/nZrO2 in the field of bone tissue engineering in detail. Many reports so far exemplify the importance of ceramics in bone regeneration. The effect of nanoceramics over native ceramics in developing composites, its role in osteogenesis etc. are the gist of this review.
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Santiesteban DY, Kubelick K, Dhada KS, Dumani D, Suggs L, Emelianov S. Monitoring/Imaging and Regenerative Agents for Enhancing Tissue Engineering Characterization and Therapies. Ann Biomed Eng 2016; 44:750-72. [PMID: 26692081 PMCID: PMC4956083 DOI: 10.1007/s10439-015-1509-y] [Citation(s) in RCA: 17] [Impact Index Per Article: 1.9] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Grants] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 08/25/2015] [Accepted: 11/11/2015] [Indexed: 01/07/2023]
Abstract
The past three decades have seen numerous advances in tissue engineering and regenerative medicine (TERM) therapies. However, despite the successes there is still much to be done before TERM therapies become commonplace in clinic. One of the main obstacles is the lack of knowledge regarding complex tissue engineering processes. Imaging strategies, in conjunction with exogenous contrast agents, can aid in this endeavor by assessing in vivo therapeutic progress. The ability to uncover real-time treatment progress will help shed light on the complex tissue engineering processes and lead to development of improved, adaptive treatments. More importantly, the utilized exogenous contrast agents can double as therapeutic agents. Proper use of these Monitoring/Imaging and Regenerative Agents (MIRAs) can help increase TERM therapy successes and allow for clinical translation. While other fields have exploited similar particles for combining diagnostics and therapy, MIRA research is still in its beginning stages with much of the current research being focused on imaging or therapeutic applications, separately. Advancing MIRA research will have numerous impacts on achieving clinical translations of TERM therapies. Therefore, it is our goal to highlight current MIRA progress and suggest future research that can lead to effective TERM treatments.
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Affiliation(s)
- Daniela Y Santiesteban
- Department of Biomedical Engineering, University of Texas at Austin, 107 W. Dean Keeton, BME Building, 1 University Station, C0800, Austin, TX, 78712, USA
- Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology, Emory University School of Medicine, 313 Ferst Dr NW, Atlanta, GA, 30332, USA
| | - Kelsey Kubelick
- School of Electrical and Computer Engineering, Georgia Institute of Technology, 777 Atlantic Drive NW, Atlanta, GA, 30332, USA
- Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology, Emory University School of Medicine, 313 Ferst Dr NW, Atlanta, GA, 30332, USA
| | - Kabir S Dhada
- Department of Biomedical Engineering, University of Texas at Austin, 107 W. Dean Keeton, BME Building, 1 University Station, C0800, Austin, TX, 78712, USA
| | - Diego Dumani
- School of Electrical and Computer Engineering, Georgia Institute of Technology, 777 Atlantic Drive NW, Atlanta, GA, 30332, USA
- Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology, Emory University School of Medicine, 313 Ferst Dr NW, Atlanta, GA, 30332, USA
| | - Laura Suggs
- Department of Biomedical Engineering, University of Texas at Austin, 107 W. Dean Keeton, BME Building, 1 University Station, C0800, Austin, TX, 78712, USA.
| | - Stanislav Emelianov
- School of Electrical and Computer Engineering, Georgia Institute of Technology, 777 Atlantic Drive NW, Atlanta, GA, 30332, USA.
- Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology, Emory University School of Medicine, 313 Ferst Dr NW, Atlanta, GA, 30332, USA.
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Ding Y, Yao Q, Li W, Schubert DW, Boccaccini AR, Roether JA. The evaluation of physical properties and in vitro cell behavior of PHB/PCL/sol–gel derived silica hybrid scaffolds and PHB/PCL/fumed silica composite scaffolds. Colloids Surf B Biointerfaces 2015; 136:93-8. [DOI: 10.1016/j.colsurfb.2015.08.023] [Citation(s) in RCA: 23] [Impact Index Per Article: 2.3] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 03/22/2015] [Revised: 08/15/2015] [Accepted: 08/18/2015] [Indexed: 10/23/2022]
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Uskoković V. When 1+1>2: Nanostructured composites for hard tissue engineering applications. MATERIALS SCIENCE & ENGINEERING. C, MATERIALS FOR BIOLOGICAL APPLICATIONS 2015; 57:434-51. [PMID: 26354283 PMCID: PMC4567690 DOI: 10.1016/j.msec.2015.07.050] [Citation(s) in RCA: 27] [Impact Index Per Article: 2.7] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Grants] [Track Full Text] [Subscribe] [Scholar Register] [Received: 12/29/2014] [Revised: 04/15/2015] [Accepted: 07/23/2015] [Indexed: 12/20/2022]
Abstract
Multicomponent, synergistic and multifunctional nanostructures have taken over the spotlight in the realm of biomedical nanotechnologies. The most prospective materials for bone regeneration today are almost exclusively composites comprising two or more components that compensate for the shortcomings of each one of them alone. This is quite natural in view of the fact that all hard tissues in the human body, except perhaps the tooth enamel, are composite nanostructures. This review article highlights some of the most prospective breakthroughs made in this research direction, with the hard tissues in main focus being those comprising bone, tooth cementum, dentin and enamel. The major obstacles to creating collagen/apatite composites modeled after the structure of bone are mentioned, including the immunogenicity of xenogeneic collagen and continuously failing attempts to replicate the biomineralization process in vitro. Composites comprising a polymeric component and calcium phosphate are discussed in light of their ability to emulate the soft/hard composite structure of bone. Hard tissue engineering composites created using hard material components other than calcium phosphates, including silica, metals and several types of nanotubes, are also discoursed on, alongside additional components deliverable using these materials, such as cells, growth factors, peptides, antibiotics, antiresorptive and anabolic agents, pharmacokinetic conjugates and various cell-specific targeting moieties. It is concluded that a variety of hard tissue structures in the body necessitates a similar variety of biomaterials for their regeneration. The ongoing development of nanocomposites for bone restoration will result in smart, theranostic materials, capable of acting therapeutically in direct feedback with the outcome of in situ disease monitoring at the cellular and subcellular scales. Progress in this research direction is expected to take us to the next generation of biomaterials, designed with the purpose of fulfilling Daedalus' dream - not restoring the tissues, but rather augmenting them.
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Affiliation(s)
- Vuk Uskoković
- Advanced Materials and Nanobiotechnology Laboratory, Department of Bioengineering, University of Illinois, Chicago, IL, USA.
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Abou Neel EA, Bozec L, Perez RA, Kim HW, Knowles JC. Nanotechnology in dentistry: prevention, diagnosis, and therapy. Int J Nanomedicine 2015; 10:6371-94. [PMID: 26504385 PMCID: PMC4605240 DOI: 10.2147/ijn.s86033] [Citation(s) in RCA: 52] [Impact Index Per Article: 5.2] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 12/21/2022] Open
Abstract
Nanotechnology has rapidly expanded into all areas of science; it offers significant alternative ways to solve scientific and medical questions and problems. In dentistry, nanotechnology has been exploited in the development of restorative materials with some significant success. This review discusses nanointerfaces that could compromise the longevity of dental restorations, and how nanotechnolgy has been employed to modify them for providing long-term successful restorations. It also focuses on some challenging areas in dentistry, eg, oral biofilm and cancers, and how nanotechnology overcomes these challenges. The recent advances in nanodentistry and innovations in oral health-related diagnostic, preventive, and therapeutic methods required to maintain and obtain perfect oral health, have been discussed. The recent advances in nanotechnology could hold promise in bringing a paradigm shift in dental field. Although there are numerous complex therapies being developed to treat many diseases, their clinical use requires careful consideration of the expense of synthesis and implementation.
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Affiliation(s)
- Ensanya Ali Abou Neel
- Division of Biomaterials, Operative Dentistry Department, Faculty of Dentistry, King Abdulaziz University, Jeddah, Saudi Arabia
- Biomaterials Department, Faculty of Dentistry, Tanta University, Tanta, Egypt
- UCL Eastman Dental Institute, Biomaterials and Tissue Engineering, London, UK
| | - Laurent Bozec
- UCL Eastman Dental Institute, Biomaterials and Tissue Engineering, London, UK
| | - Roman A Perez
- Institute of Tissue Regenerative Engineering (ITREN), Dankook University, Cheonan, Republic of Korea
- Department of Nanobiomedical Science and BK21 Plus NBM Global Research Center for Regenerative Medicine, Dankook University, Cheonan, Republic of Korea
| | - Hae-Won Kim
- Institute of Tissue Regenerative Engineering (ITREN), Dankook University, Cheonan, Republic of Korea
- Department of Nanobiomedical Science and BK21 Plus NBM Global Research Center for Regenerative Medicine, Dankook University, Cheonan, Republic of Korea
- Department of Biomaterials Science, College of Dentistry, Dankook University, Cheonan, Republic of Korea
| | - Jonathan C Knowles
- UCL Eastman Dental Institute, Biomaterials and Tissue Engineering, London, UK
- Department of Nanobiomedical Science and BK21 Plus NBM Global Research Center for Regenerative Medicine, Dankook University, Cheonan, Republic of Korea
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Electrospun aligned PLGA and PLGA/gelatin nanofibers embedded with silica nanoparticles for tissue engineering. Int J Biol Macromol 2015; 79:687-95. [DOI: 10.1016/j.ijbiomac.2015.05.050] [Citation(s) in RCA: 122] [Impact Index Per Article: 12.2] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 02/07/2015] [Revised: 05/23/2015] [Accepted: 05/28/2015] [Indexed: 01/03/2023]
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Trujillo S, Pérez-Román E, Kyritsis A, Gómez Ribelles JL, Pandis C. Organic-inorganic bonding in chitosan-silica hybrid networks: Physical properties. ACTA ACUST UNITED AC 2015. [DOI: 10.1002/polb.23774] [Citation(s) in RCA: 20] [Impact Index Per Article: 2.0] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 12/19/2022]
Affiliation(s)
- Sara Trujillo
- Centre for Biomaterials and Tissue Engineering, CBIT, Universitat Politècnica de València; Camino de Vera s/n 46022 Valencia Spain
| | - Estela Pérez-Román
- Centre for Biomaterials and Tissue Engineering, CBIT, Universitat Politècnica de València; Camino de Vera s/n 46022 Valencia Spain
| | - Apostolos Kyritsis
- Department of Physics; National Technical University of Athens; Zografou Campus 15780 Athens Greece
| | - José Luis Gómez Ribelles
- Centre for Biomaterials and Tissue Engineering, CBIT, Universitat Politècnica de València; Camino de Vera s/n 46022 Valencia Spain
- CIBER in Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN); Spain
| | - Christos Pandis
- Centre for Biomaterials and Tissue Engineering, CBIT, Universitat Politècnica de València; Camino de Vera s/n 46022 Valencia Spain
- Department of Physics; National Technical University of Athens; Zografou Campus 15780 Athens Greece
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Motamedian SR, Hosseinpour S, Ahsaie MG, Khojasteh A. Smart scaffolds in bone tissue engineering: A systematic review of literature. World J Stem Cells 2015; 7:657-668. [PMID: 25914772 PMCID: PMC4404400 DOI: 10.4252/wjsc.v7.i3.657] [Citation(s) in RCA: 86] [Impact Index Per Article: 8.6] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Journal Information] [Submit a Manuscript] [Subscribe] [Scholar Register] [Received: 07/29/2014] [Revised: 12/10/2014] [Accepted: 12/29/2014] [Indexed: 02/06/2023] Open
Abstract
AIM To improve osteogenic differentiation and attachment of cells. METHODS An electronic search was conducted in PubMed from January 2004 to December 2013. Studies which performed smart modifications on conventional bone scaffold materials were included. Scaffolds with controlled release or encapsulation of bioactive molecules were not included. Experiments which did not investigate response of cells toward the scaffold (cell attachment, proliferation or osteoblastic differentiation) were excluded. RESULTS Among 1458 studies, 38 met the inclusion and exclusion criteria. The main scaffold varied extensively among the included studies. Smart modifications included addition of growth factors (group I-11 studies), extracellular matrix-like molecules (group II-13 studies) and nanoparticles (nano-HA) (group III-17 studies). In all groups, surface coating was the most commonly applied approach for smart modification of scaffolds. In group I, bone morphogenetic proteins were mainly used as growth factor stabilized on polycaprolactone (PCL). In group II, collagen 1 in combination with PCL, hydroxyapatite (HA) and tricalcium phosphate were the most frequent scaffolds used. In the third group, nano-HA with PCL and chitosan were used the most. As variable methods were used, a thorough and comprehensible compare between the results and approaches was unattainable. CONCLUSION Regarding the variability in methodology of these in vitro studies it was demonstrated that smart modification of scaffolds can improve tissue properties.
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Affiliation(s)
- Saeed Reza Motamedian
- Saeed Reza Motamedian, Research Institute of Dental Sciences, School of Dentistry, Shahid Beheshti University of Medical Sciences, Tehran PO Box 19839, Iran
| | - Sepanta Hosseinpour
- Saeed Reza Motamedian, Research Institute of Dental Sciences, School of Dentistry, Shahid Beheshti University of Medical Sciences, Tehran PO Box 19839, Iran
| | - Mitra Ghazizadeh Ahsaie
- Saeed Reza Motamedian, Research Institute of Dental Sciences, School of Dentistry, Shahid Beheshti University of Medical Sciences, Tehran PO Box 19839, Iran
| | - Arash Khojasteh
- Saeed Reza Motamedian, Research Institute of Dental Sciences, School of Dentistry, Shahid Beheshti University of Medical Sciences, Tehran PO Box 19839, Iran
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Pelipenko J, Kocbek P, Kristl J. Critical attributes of nanofibers: Preparation, drug loading, and tissue regeneration. Int J Pharm 2015; 484:57-74. [DOI: 10.1016/j.ijpharm.2015.02.043] [Citation(s) in RCA: 154] [Impact Index Per Article: 15.4] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 11/18/2014] [Revised: 02/16/2015] [Accepted: 02/16/2015] [Indexed: 12/13/2022]
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Carrow JK, Gaharwar AK. Bioinspired Polymeric Nanocomposites for Regenerative Medicine. MACROMOL CHEM PHYS 2014. [DOI: 10.1002/macp.201400427] [Citation(s) in RCA: 102] [Impact Index Per Article: 9.3] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 01/09/2023]
Affiliation(s)
- James K. Carrow
- Department of Biomedical Engineering; Texas A&M University; College Station TX 77843 USA
| | - Akhilesh K. Gaharwar
- Department of Biomedical Engineering; Texas A&M University; College Station TX 77843 USA
- Department of Materials Science and Engineering; Texas A&M University; College Station TX 77843 USA
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Patil S, Paul S. A comprehensive review on the role of various materials in the osteogenic differentiation of mesenchymal stem cells with a special focus on the association of heat shock proteins and nanoparticles. Cells Tissues Organs 2014; 199:81-102. [PMID: 25401759 DOI: 10.1159/000362226] [Citation(s) in RCA: 10] [Impact Index Per Article: 0.9] [Reference Citation Analysis] [Abstract] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Accepted: 03/13/2014] [Indexed: 11/19/2022] Open
Abstract
Mesenchymal stem cells (MSCs) have important roles in the area of regenerative medicine and clinical applications due to their pluripotent nature. Osteogenic differentiation of MSCs has been studied extensively using various stimulants to develop models of bone repair. There are several factors that enhance the differentiation of MSCs into bone tissues. This review focuses on the effects of various inducers on the osteoblast differentiation of MSCs at different stages of cellular development. We discuss the various growth factors, hormones, vitamins, cytokines, chemical stimulants, and mechanical forces applied in bioreactors that play an essential role in the proliferation, differentiation, and matrix mineralization of stem cells during osteogenesis. Various nanoparticles have also been used recently for the same purpose and the results are promising. Moreover, we review the role of various stresses, including thermal stress, and the subsequent involvement of heat shock proteins as inducers of the proliferation and differentiation of osteoblasts. We also report how various proteasome inhibitors have been shown to induce proliferation and osteogenic differentiation of MSCs in a number of cases. In this communication, the role of peptide-based scaffolds in osteoblast proliferation and differentiation is also reviewed. Based on the reviewed information, this article proposes novel possibilities for the enhancement of proliferation, differentiation, and migration of osteoblasts from MSCs. © 2014 S. Karger AG, Basel.
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Affiliation(s)
- Supriya Patil
- Structural Biology and Nanomedicine Laboratory, Department of Biotechnology and Medical Engineering, National Institute of Technology Rourkela, Rourkela, India
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Rodella LF, Bonazza V, Labanca M, Lonati C, Rezzani R. A review of the effects of dietary silicon intake on bone homeostasis and regeneration. J Nutr Health Aging 2014; 18:820-6. [PMID: 25389960 DOI: 10.1007/s12603-014-0555-8] [Citation(s) in RCA: 27] [Impact Index Per Article: 2.5] [Reference Citation Analysis] [Abstract] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 02/07/2023]
Abstract
OBJECTIVE Increasing evidences suggest that dietary Silicon (Si) intake, is positively correlated with bone homeostasis and regeneration, representing a potential and valid support for the prevention and improvement of bone diseases, like osteoporosis. This review, aims to provide the state of art of the studies performed until today, in order to investigate and clarify the beneficial properties and effects of silicates, on bone metabolism. METHODS We conducted a systematic literature search up to March 2013, using two medical databases (Pubmed and the Cochrane Library), to review the studies about Si consumption and bone metabolism. RESULTS We found 45 articles, but 38 were specifically focused on Si studies. CONCLUSION RESULTS showed a positive relationship between dietary Si intake and bone regeneration.
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Affiliation(s)
- L F Rodella
- Rodella Luigi Fabrizio, Section of Anatomy and Physiopathology, Department of Clinical and Experimental Sciences, University of Brescia, V.le Europa 11, 25123 Brescia, Italy. Phone +390303717485 Fax +390303717486 e-mail
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Ganesh N, Ashokan A, Rajeshkannan R, Chennazhi K, Koyakutty M, Nair SV. Magnetic resonance functional nano-hydroxyapatite incorporated poly(caprolactone) composite scaffolds for in situ monitoring of bone tissue regeneration by MRI. Tissue Eng Part A 2014; 20:2783-94. [PMID: 24785187 DOI: 10.1089/ten.tea.2014.0161] [Citation(s) in RCA: 28] [Impact Index Per Article: 2.5] [Reference Citation Analysis] [Abstract] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 11/12/2022] Open
Abstract
In this study, we have reported the incorporation of a multi-modal contrast agent based on hydroxyapatite nanocrystals, within a poly(caprolactone)(PCL) nanofibrous scaffold by electrospinning. The multifunctional hydroxyapatite nanoparticles (MF-nHAp) showed simultaneous contrast enhancement for three major molecular imaging techniques. In this article, the magnetic resonance (MR) contrast enhancement ability of the MF-nHAp was exploited for the purpose of potentially monitoring as well as for influencing tissue regeneration. These MF-nHAp containing PCL scaffolds were engineered in order to enhance the osteogenic potential as well as its MR functionality for their application in bone tissue engineering. The nano-composite scaffolds along with pristine PCL were evaluated physico-chemically and biologically in vitro, in the presence of human mesenchymal stem cells (hMSCs). The incorporation of 30-40 nm sized MF-nHAp within the nanofibers showed a substantial increase in scaffold strength, protein adsorption, proliferation, and osteogenic differentiation of hMSCs along with enhanced MR functionality. This preliminary study was performed to eventually exploit the MR contrast imaging capability of MF-nHAp in nanofibrous scaffolds for real-time imaging of the changes in the tissue engineered construct.
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Affiliation(s)
- Nitya Ganesh
- 1 Amrita Centre for Nanosciences and Molecular Medicine, Amrita Institute of Medical Sciences and Research Centre, Amrita Vishwa Vidyapeetham University , Kochi, India
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Pandis C, Trujillo S, Roganowicz M, Gómez Ribelles JL. Hybrid Polycaprolactone/Silica Porous Membranes Produced by Sol-Gel. ACTA ACUST UNITED AC 2014. [DOI: 10.1002/masy.201300155] [Citation(s) in RCA: 7] [Impact Index Per Article: 0.6] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 11/12/2022]
Affiliation(s)
- Christos Pandis
- Physics Department; National Technical University of Athens; Zografou Campus, GR-15780 Athens Greece
- Centro de Biomateriales e Ingeniería Tisular (CBIT); Universitat Politècnica de València; Cno. de Verna s/n, E-46071 Valencia Spain
| | - Sara Trujillo
- Centro de Biomateriales e Ingeniería Tisular (CBIT); Universitat Politècnica de València; Cno. de Verna s/n, E-46071 Valencia Spain
| | - Marcin Roganowicz
- Centro de Biomateriales e Ingeniería Tisular (CBIT); Universitat Politècnica de València; Cno. de Verna s/n, E-46071 Valencia Spain
| | - José Luis Gómez Ribelles
- Centro de Biomateriales e Ingeniería Tisular (CBIT); Universitat Politècnica de València; Cno. de Verna s/n, E-46071 Valencia Spain
- Ciber en Bioingeniería; Biomateriales y Nanomedicina (CIBER-BBN); Valencia Spain
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Gaharwar AK, Mukundan S, Karaca E, Dolatshahi-Pirouz A, Patel A, Rangarajan K, Mihaila SM, Iviglia G, Zhang H, Khademhosseini A. Nanoclay-enriched poly(ɛ-caprolactone) electrospun scaffolds for osteogenic differentiation of human mesenchymal stem cells. Tissue Eng Part A 2014; 20:2088-101. [PMID: 24842693 DOI: 10.1089/ten.tea.2013.0281] [Citation(s) in RCA: 106] [Impact Index Per Article: 9.6] [Reference Citation Analysis] [Abstract] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 11/13/2022] Open
Abstract
Musculoskeletal tissue engineering aims at repairing and regenerating damaged tissues using biological tissue substitutes. One approach to achieve this aim is to develop osteoconductive scaffolds that facilitate the formation of functional bone tissue. We have fabricated nanoclay-enriched electrospun poly(ɛ-caprolactone) (PCL) scaffolds for osteogenic differentiation of human mesenchymal stem cells (hMSCs). A range of electrospun scaffolds is fabricated by varying the nanoclay concentrations within the PCL scaffolds. The addition of nanoclay decreases fiber diameter and increases surface roughness of electrospun fibers. The enrichment of PCL scaffold with nanoclay promotes in vitro biomineralization when subjected to simulated body fluid (SBF), indicating bioactive characteristics of the hybrid scaffolds. The degradation rate of PCL increases due to the addition of nanoclay. In addition, a significant increase in crystallization temperature of PCL is also observed due to enhanced surface interactions between PCL and nanoclay. The effect of nanoclay on the mechanical properties of electrospun fibers is also evaluated. The feasibility of using nanoclay-enriched PCL scaffolds for tissue engineering applications is investigated in vitro using hMSCs. The nanoclay-enriched electrospun PCL scaffolds support hMSCs adhesion and proliferation. The addition of nanoclay significantly enhances osteogenic differentiation of hMSCs on the electrospun scaffolds as evident by an increase in alkaline phosphates activity of hMSCs and higher deposition of mineralized extracellular matrix compared to PCL scaffolds. Given its unique bioactive characteristics, nanoclay-enriched PCL fibrous scaffold may be used for musculoskeletal tissue engineering.
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Affiliation(s)
- Akhilesh K Gaharwar
- 1 David H. Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology , Cambridge, Massachusetts
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López-Cebral R, Martín-Pastor M, Seijo B, Sanchez A. Progress in the characterization of bio-functionalized nanoparticles using NMR methods and their applications as MRI contrast agents. PROGRESS IN NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY 2014; 79:1-13. [PMID: 24815362 DOI: 10.1016/j.pnmrs.2014.01.002] [Citation(s) in RCA: 14] [Impact Index Per Article: 1.3] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Subscribe] [Scholar Register] [Received: 05/23/2013] [Revised: 01/13/2014] [Accepted: 01/15/2014] [Indexed: 05/22/2023]
Abstract
Significant progress has been made over the last three decades in the field of NMR, a technique which has proven to have a variety of applications in many scientific disciplines, including nanotechnology. Herein we describe how NMR enables the characterization of nanosystems at different stages of their formation and modification (raw materials, bare or functionalized nanosystems), even making it possible to study in vivo nanoparticle interactions, thereby importantly contributing to nanoparticle design and subsequent optimization. Furthermore, the unique characteristics of nanosystems can open up new prospects for site-targeted, more specific contrast agents, contributing to the development of certain nuclear magnetic resonance applications such as MRI.
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Affiliation(s)
- Rita López-Cebral
- Department of Pharmacy and Pharmaceutical Technology, Faculty of Pharmacy, University of Santiago de Compostela (USC), Campus Sur, 15782 Santiago de Compostela, Spain
| | - Manuel Martín-Pastor
- Nuclear Magnetic Resonance Unit, RIADT, University of Santiago de Compostela (USC), Campus Vida, 15706 Santiago de Compostela, Spain
| | - Begoña Seijo
- Department of Pharmacy and Pharmaceutical Technology, Faculty of Pharmacy, University of Santiago de Compostela (USC), Campus Sur, 15782 Santiago de Compostela, Spain; Molecular ImageGroup, IDIS, Santiago de Compostela University Hospital Complex (CHUS), A Choupana, 15706 Santiago de Compostela, Spain
| | - Alejandro Sanchez
- Department of Pharmacy and Pharmaceutical Technology, Faculty of Pharmacy, University of Santiago de Compostela (USC), Campus Sur, 15782 Santiago de Compostela, Spain; Molecular ImageGroup, IDIS, Santiago de Compostela University Hospital Complex (CHUS), A Choupana, 15706 Santiago de Compostela, Spain.
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Allo BA, Lin S, Mequanint K, Rizkalla AS. Role of bioactive 3D hybrid fibrous scaffolds on mechanical behavior and spatiotemporal osteoblast gene expression. ACS APPLIED MATERIALS & INTERFACES 2013; 5:7574-7583. [PMID: 23826710 DOI: 10.1021/am401861w] [Citation(s) in RCA: 22] [Impact Index Per Article: 1.8] [Reference Citation Analysis] [Abstract] [MESH Headings] [Grants] [Track Full Text] [Subscribe] [Scholar Register] [Indexed: 06/02/2023]
Abstract
Three-dimensional (3D) bioactive organic-inorganic (O/I) hybrid fibrous scaffolds are attractive extracellular matrix (ECM) surrogates for bone tissue engineering. With the aim of regulating osteoblast gene expression in 3D, a new class of hybrid fibrous scaffolds with two distinct fiber diameters (260 and 600 nm) and excellent physico-mechanical properties were fabricated from tertiary (SiO2-CaO-P2O5) bioactive glass (BG) and poly (ε-caprolactone) (PCL) by in situ sol-gel and electrospinning process. The PCL/BG hybrid fibrous scaffolds exhibited accelerated wetting properties, enhanced pore sizes and porosity, and superior mechanical properties that were dependent on fiber diameter. Contrary to control PCL fibrous scaffolds that were devoid of bonelike apatite particles, incubating PCL/BG hybrid fibrous scaffolds in simulated body fluid (SBF) revealed bonelike apatite deposition. Osteoblast cells cultured on PCL/BG hybrid fibrous scaffolds spread with multiple attachments and actively proliferated suggesting that the low temperature in situ sol-gel and electrospinning process did not have a detrimental effect. Targeted bone-associated gene expressions by rat calvarial osteoblasts seeded on these hybrid scaffolds demonstrated remarkable spatiotemporal gene activation. Transcriptional-level gene expressions for alkaline phosphatase (ALP), osteopontin (OPN), bone sialoprotein (BSP), and osteocalcin (OCN) were significantly higher on the hybrid fibrous scaffolds (p < 0.001) that were largely dependent on fiber diameter compared. Taken together, our results suggest that PCL/BG fibrous scaffolds may accelerate bone formation by providing a favorable microenvironment.
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Affiliation(s)
- Bedilu A Allo
- Department of Chemical and Biochemical Engineering, The University of Western Ontario, London, Ontario, Canada
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