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Sabira O, Ajaykumar AP, Varma SR, Jayaraj KN, Kotakonda M, Kumar P, Vaikkathillam P, Sivadasan Binitha V, Alen AP, Raghu AV, Zeena KV. Nepenthes pitcher fluid for the green synthesis of silver nanoparticles with biofilm inhibition, anticancer and antioxidant properties. Sci Rep 2025; 15:5349. [PMID: 39948126 PMCID: PMC11825719 DOI: 10.1038/s41598-025-89212-9] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 09/23/2024] [Accepted: 02/04/2025] [Indexed: 02/16/2025] Open
Abstract
This is the first report of silver nanoparticles (AgNPs) synthesis utilizing the pitcher secretion from an insectivorous plant, specifically Nepenthes ventrata, through a microwave assisted green synthesis approach. The successful formation of AgNPs was validated through a comprehensive set of analyses, including UV-Vis spectroscopy, Fourier-transform infrared spectroscopy, transmission electron microscopy, DLS analysis and Zeta potential measurements. In addition gas chromatography-high-resolution mass spectrometry and liquid chromatography-high-resolution mass spectrometry analyses were conducted to examine the components present in the pitcher secretion. These analyses aimed to identify the capping and stabilizing agents in the secretion that facilitate the synthesis and stability of AgNPs. The synthesized AgNPs significantly inhibited biofilm formation by Pseudomonas aeruginosa PAO1, as demonstrated by Crystal Violet staining and fluorescence microscopy. Additionally, these AgNPs showed promising antioxidant properties through a DPPH radical scavenging assay. Furthermore, the anticancer properties of the AgNPs were analyzed using an MTT assay, which measures cell metabolic activity as an indicator of cell viability, proliferation, and cytotoxicity. Collectively, these findings suggest that the biosynthesized AgNPs possess multifaceted biological applications, showcasing their utility as both antimicrobial and antioxidative agents, and highlighting their potential in medical and environmental applications.
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Affiliation(s)
- Ovungal Sabira
- Division of Biomaterial Sciences, Department of Zoology, Sree Neelakanta Government Sanskrit College, Pattambi, Palakkad, Kerala, India
| | - Anthyalam Parambil Ajaykumar
- Division of Biomaterial Sciences, Department of Zoology, Sree Neelakanta Government Sanskrit College, Pattambi, Palakkad, Kerala, India.
| | - Sudhir Rama Varma
- Clinical Sciences Department, Centre for Medical and Bio-Allied Health Sciences Research, Ajman University, Ajman, United Arab Emirates
| | - Kodangattil Narayanan Jayaraj
- Basic Medical and Dental Sciences Department, Centre for Medical and Bio-Allied Health Sciences Research, Ajman University, Ajman, United Arab Emirates.
| | | | - Praveen Kumar
- Department of Zoology, Government College for Women, Thiruvananthapuram, Kerala, India
| | | | | | - Alex Philip Alen
- Department of Botany, Government Victoria College, Palakkad, Kerala, India
| | - A V Raghu
- Kerala Forest Research Institute, Peechi, Thrissur, Kerala, 680653, India
| | - Koladath Vasu Zeena
- Division of Biomaterial Sciences, Department of Zoology, Sree Neelakanta Government Sanskrit College, Pattambi, Palakkad, Kerala, India
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Lam DL, Cheng YT, Huang CJ. Biodegradable and pH-Responsive Amphiphilic Poly(succinimide) Derivatives for Triggered Release of Antibiotics for Management of Infected Wounds. ACS APPLIED MATERIALS & INTERFACES 2023; 15:53297-53309. [PMID: 37947480 DOI: 10.1021/acsami.3c12939] [Citation(s) in RCA: 2] [Impact Index Per Article: 1.0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Subscribe] [Scholar Register] [Indexed: 11/12/2023]
Abstract
Wound infection has become a healthy economic burden globally. Current wound management mainly relies on the use of antibiotics; however, the misuse and overuse of antibiotics can easily result in antibiotic resistance. This study proposes a biodegradable, biocompatible, and pH-responsive amphiphilic 11-aminoundecanoic acid-grafted polysuccinimide (AUA-PSI) as a nanocarrier for drug encapsulation via nanoprecipitation. The succinimide groups in the backbone of PSI allow facile postfunctionalization via an aminolysis reaction. The degree of substitution of AUA can be modulated to adjust the degradation rate, pH sensitivity, and drug-release profile. Antibiotic rifampicin was incorporated with AUA-PSI to form Rif-AUA-PSI nanoparticles and demonstrated pH-responsiveness and antimicrobial activity. Because of the elevation of the pH value from pH = ∼ 5.5 in healthy skin to pH > 7 in an infected wound, Rif-AUA-PSI nanoparticles begin to decompose and release Rif upon the hydrolysis of succinimide/amide and deprotonation of carboxyl groups. The effective suppression of bacterial growth by Rif-AUA-PSI nanoparticles was demonstrated using a plate count method. More importantly, Rif-AUA-PSI nanoparticles were physically deposited on cotton gauze bandages as an antibiotic wound dressing. The Rif-AUA-PSI-modified gauze was applied to infected wounds on rats for wound management. The results show fast wound healing and inhibition of bacterial growth, which demonstrate that the method promotes modulable amphiphilicity, biodegradability, biocompatibility, pH-responsiveness, and facile modification for nanomedicine and medical devices.
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Affiliation(s)
- Dieu-Linh Lam
- Department of Chemical and Materials Engineering, National Central University, Jhong-Li, Taoyuan 320, Taiwan
- Department of Biomedical Sciences and Engineering, National Central University, Jhong-Li, Taoyuan 320, Taiwan
| | - Ying-Tzu Cheng
- Department of Chemical and Materials Engineering, National Central University, Jhong-Li, Taoyuan 320, Taiwan
| | - Chun-Jen Huang
- Department of Chemical and Materials Engineering, National Central University, Jhong-Li, Taoyuan 320, Taiwan
- R&D Center for Membrane Technology, Chung Yuan Christian University, 200 Chung Pei Rd., Chung-Li City 32023, Taiwan
- NCU-Covestro Research Center, National Central University, Jhong-Li, Taoyuan 320, Taiwan
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Preparation of magnetic fluorescent nanoparticles with quaternary ammonium chitosan derivatives and its properties. Colloids Surf A Physicochem Eng Asp 2023. [DOI: 10.1016/j.colsurfa.2022.130310] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 11/07/2022]
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Bychkova AV, Yakunina MN, Lopukhova MV, Degtyarev YN, Motyakin MV, Pokrovsky VS, Kovarski AL, Gorobets MG, Retivov VM, Khachatryan DS. Albumin-Functionalized Iron Oxide Nanoparticles for Theranostics: Engineering and Long-Term In Situ Imaging. Pharmaceutics 2022; 14:2771. [PMID: 36559265 PMCID: PMC9782891 DOI: 10.3390/pharmaceutics14122771] [Citation(s) in RCA: 2] [Impact Index Per Article: 0.7] [Reference Citation Analysis] [Abstract] [Key Words] [Grants] [Track Full Text] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 11/02/2022] [Revised: 12/05/2022] [Accepted: 12/08/2022] [Indexed: 12/14/2022] Open
Abstract
Magnetic nanosystems (MNSs) consisting of magnetic iron oxide nanoparticles (IONPs) coated by human serum albumin (HSA), commonly used as a component of hybrid nanosystems for theranostics, were engineered and characterized. The HSA coating was obtained by means of adsorption and free radical modification of the protein molecules on the surface of IONPs exhibiting peroxidase-like activity. The generation of hydroxyl radicals in the reaction of IONPs with hydrogen peroxide was proven by the spin trap technique. The methods of dynamic light scattering (DLS) and electron magnetic resonance (EMR) were applied to confirm the stability of the coatings formed on the surface of the IONPs. The synthesized MNSs (d ~35 nm by DLS) were intraarterially administered in tumors implanted to rats in the dose range from 20 to 60 μg per animal and studied in vivo as a contrasting agent for computed tomography. The long-term (within 14 days of the experiment) presence of the MNSs in the tumor vascular bed was detected without immediate or delayed adverse reactions and significant systemic toxic effects during the observation period. The peroxidase-like activity of MNSs was proven by the colorimetric test with o-phenylenediamine (OPD) as a substrate. The potential of the synthesized MNSs to be used for theranostics, particularly, in oncology, was discussed.
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Affiliation(s)
- Anna V. Bychkova
- Emanuel Institute of Biochemical Physics of Russian Academy of Sciences, 4, Kosygina Str., Moscow 119334, Russia
| | - Marina N. Yakunina
- N.N. Blokhin National Medical Research Center of Oncology, 24, Kashirskoye Sh., Moscow 115478, Russia
| | - Mariia V. Lopukhova
- Emanuel Institute of Biochemical Physics of Russian Academy of Sciences, 4, Kosygina Str., Moscow 119334, Russia
| | - Yevgeniy N. Degtyarev
- Emanuel Institute of Biochemical Physics of Russian Academy of Sciences, 4, Kosygina Str., Moscow 119334, Russia
- N.N. Semenov Federal Research Center for Chemical Physics, Russian Academy of Sciences, 4, Kosygina Str., Moscow 119991, Russia
| | - Mikhail V. Motyakin
- Emanuel Institute of Biochemical Physics of Russian Academy of Sciences, 4, Kosygina Str., Moscow 119334, Russia
- N.N. Semenov Federal Research Center for Chemical Physics, Russian Academy of Sciences, 4, Kosygina Str., Moscow 119991, Russia
| | - Vadim S. Pokrovsky
- Emanuel Institute of Biochemical Physics of Russian Academy of Sciences, 4, Kosygina Str., Moscow 119334, Russia
- N.N. Blokhin National Medical Research Center of Oncology, 24, Kashirskoye Sh., Moscow 115478, Russia
- Laboratory of Experimental Oncology, Research Institute of Molecular and Cellular Medicine, RUDN University, 6, Miklukho-Maklaya Str., Moscow 117198, Russia
- Department of Biotechnology, Sirius University of Science and Technology, 1, Olympic Pr., Federal Territory Sirius, Krasnodarsky Kray, Sochi 354340, Russia
| | - Alexander L. Kovarski
- Emanuel Institute of Biochemical Physics of Russian Academy of Sciences, 4, Kosygina Str., Moscow 119334, Russia
| | - Maria G. Gorobets
- Emanuel Institute of Biochemical Physics of Russian Academy of Sciences, 4, Kosygina Str., Moscow 119334, Russia
| | - Vasily M. Retivov
- The Federal State Unitary Enterprise, Institute of Chemical Reagents and High Purity Chemical Substances of National Research Center “Kurchatov Institute”, 3, Bogorodsky Val, Moscow 107076, Russia
- National Research Center “Kurchatov Institute”, 1, Akademika Kurchatova pl., Moscow 123182, Russia
| | - Derenik S. Khachatryan
- The Federal State Unitary Enterprise, Institute of Chemical Reagents and High Purity Chemical Substances of National Research Center “Kurchatov Institute”, 3, Bogorodsky Val, Moscow 107076, Russia
- National Research Center “Kurchatov Institute”, 1, Akademika Kurchatova pl., Moscow 123182, Russia
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Zheng F, Huang X, Ding J, Bi A, Wang S, Chen F, Zeng W. NIR-I Dye-Based Probe: A New Window for Bimodal Tumor Theranostics. Front Chem 2022; 10:859948. [PMID: 35402374 PMCID: PMC8984032 DOI: 10.3389/fchem.2022.859948] [Citation(s) in RCA: 16] [Impact Index Per Article: 5.3] [Reference Citation Analysis] [Abstract] [Key Words] [Grants] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 01/22/2022] [Accepted: 02/25/2022] [Indexed: 11/13/2022] Open
Abstract
Near-infrared (NIR, 650-1700 nm) bioimaging has emerged as a powerful strategy in tumor diagnosis. In particular, NIR-I fluorescence imaging (650-950 nm) has drawn more attention, benefiting from the high quantum yield and good biocompatibility. Since their biomedical applications are slightly limited by their relatively low penetration depth, NIR-I fluorescence imaging probes have been under extensive development in recent years. This review summarizes the particular application of the NIR-I fluorescent dye-contained bimodal probes, with emphasis on related nanoprobes. These probes have enabled us to overcome the drawbacks of individual imaging modalities as well as achieve synergistic imaging. Meanwhile, the application of these NIR-I fluorescence-based bimodal probes for cancer theranostics is highlighted.
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Affiliation(s)
- Fan Zheng
- Xiangya School of Pharmaceutical Sciences, Central South University, Changsha, China
- Hunan Key Laboratory of Diagnostic and Therapeutic Drug Research for Chronic Diseases, Changsha, China
| | - Xueyan Huang
- Xiangya School of Pharmaceutical Sciences, Central South University, Changsha, China
- Hunan Key Laboratory of Diagnostic and Therapeutic Drug Research for Chronic Diseases, Changsha, China
| | - Jipeng Ding
- Xiangya School of Pharmaceutical Sciences, Central South University, Changsha, China
- Hunan Key Laboratory of Diagnostic and Therapeutic Drug Research for Chronic Diseases, Changsha, China
| | - Anyao Bi
- Xiangya School of Pharmaceutical Sciences, Central South University, Changsha, China
- Hunan Key Laboratory of Diagnostic and Therapeutic Drug Research for Chronic Diseases, Changsha, China
| | - Shifen Wang
- Xiangya School of Pharmaceutical Sciences, Central South University, Changsha, China
- Hunan Key Laboratory of Diagnostic and Therapeutic Drug Research for Chronic Diseases, Changsha, China
| | - Fei Chen
- Xiangya School of Pharmaceutical Sciences, Central South University, Changsha, China
- Hunan Key Laboratory of Diagnostic and Therapeutic Drug Research for Chronic Diseases, Changsha, China
| | - Wenbin Zeng
- Xiangya School of Pharmaceutical Sciences, Central South University, Changsha, China
- Hunan Key Laboratory of Diagnostic and Therapeutic Drug Research for Chronic Diseases, Changsha, China
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Montiel Schneider MG, Martín MJ, Otarola J, Vakarelska E, Simeonov V, Lassalle V, Nedyalkova M. Biomedical Applications of Iron Oxide Nanoparticles: Current Insights Progress and Perspectives. Pharmaceutics 2022; 14:204. [PMID: 35057099 PMCID: PMC8780449 DOI: 10.3390/pharmaceutics14010204] [Citation(s) in RCA: 93] [Impact Index Per Article: 31.0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 12/09/2021] [Revised: 01/01/2022] [Accepted: 01/14/2022] [Indexed: 01/08/2023] Open
Abstract
The enormous development of nanomaterials technology and the immediate response of many areas of science, research, and practice to their possible application has led to the publication of thousands of scientific papers, books, and reports. This vast amount of information requires careful classification and order, especially for specifically targeted practical needs. Therefore, the present review aims to summarize to some extent the role of iron oxide nanoparticles in biomedical research. Summarizing the fundamental properties of the magnetic iron oxide nanoparticles, the review's next focus was to classify research studies related to applying these particles for cancer diagnostics and therapy (similar to photothermal therapy, hyperthermia), in nano theranostics, multimodal therapy. Special attention is paid to research studies dealing with the opportunities of combining different nanomaterials to achieve optimal systems for biomedical application. In this regard, original data about the synthesis and characterization of nanolipidic magnetic hybrid systems are included as an example. The last section of the review is dedicated to the capacities of magnetite-based magnetic nanoparticles for the management of oncological diseases.
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Affiliation(s)
- María Gabriela Montiel Schneider
- INQUISUR, Departamento de Química, Universidad Nacional del Sur (UNS)-CONICET, Bahía Blanca 8000, Argentina; (M.G.M.S.); (M.J.M.); (J.O.); (V.L.)
| | - María Julia Martín
- INQUISUR, Departamento de Química, Universidad Nacional del Sur (UNS)-CONICET, Bahía Blanca 8000, Argentina; (M.G.M.S.); (M.J.M.); (J.O.); (V.L.)
| | - Jessica Otarola
- INQUISUR, Departamento de Química, Universidad Nacional del Sur (UNS)-CONICET, Bahía Blanca 8000, Argentina; (M.G.M.S.); (M.J.M.); (J.O.); (V.L.)
| | - Ekaterina Vakarelska
- Faculty of Chemistry and Pharmacy, University of Sofia, 1 James Bourchier Blvd., 1164 Sofia, Bulgaria;
| | - Vasil Simeonov
- Faculty of Chemistry and Pharmacy, University of Sofia, 1 James Bourchier Blvd., 1164 Sofia, Bulgaria;
| | - Verónica Lassalle
- INQUISUR, Departamento de Química, Universidad Nacional del Sur (UNS)-CONICET, Bahía Blanca 8000, Argentina; (M.G.M.S.); (M.J.M.); (J.O.); (V.L.)
| | - Miroslava Nedyalkova
- Faculty of Chemistry and Pharmacy, University of Sofia, 1 James Bourchier Blvd., 1164 Sofia, Bulgaria;
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Effect of Ropivacaine-Loaded Magnetic Nanoparticles on Ankle Nerve Block in Rats. COMPUTATIONAL AND MATHEMATICAL METHODS IN MEDICINE 2021; 2021:8553015. [PMID: 34899971 PMCID: PMC8660227 DOI: 10.1155/2021/8553015] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Track Full Text] [Download PDF] [Figures] [Subscribe] [Scholar Register] [Received: 09/21/2021] [Accepted: 10/21/2021] [Indexed: 12/03/2022]
Abstract
Aim Our study is to determine the influence of ropivacaine-loaded magnetic nanoparticles (MNP/Rop) on ankle nerve block in rats. Materials and Methods MNP/Rop was prepared and then injected intravenously into rats to evaluate its anesthetic effect on rat limbs. Mechanical pain thresholds paw withdrawal threshold (PWT) and paw withdrawal thermal latency (PWL) were employed for the assessment of ankle nerve block in rats. Results PWT increased from T1 to T4 in each group (P < 0.05). The intergroup comparison determined no distinct difference in the PWT value among the three series at T1 (P > 0.05); however, PWT values at T2-T4 were higher in nerve block control group (NBCG) and MNP/Rop group than in blank group (BG), and they remained slightly higher in MNP/Rop group than in NBCG. The intragroup comparison revealed that from T1 to T4, PWL in each group showed a rising trend. The PWL at T1 showed no evident difference among the three series (P > 0.05); however, PWL values at T2-T4 were higher in NBCG and MNP/Rop group than in BG, and they remained slightly higher in MNP/Rop group than in NBCG. In MNP/Rop group, both PWT and PWL increased with the increase of Fe3O4 load in MNP/Rop (P < 0.05), while PWT and PWL remained unchanged when the load was 2.189%; moreover, PWT and PWL elevated as Rop concentration increased in MNP/Rop (P < 0.05), while they kept unaltered under 40 μL 1% Rop. Conclusion Intravenous injection of MNP/Rop into rats and inhalation of MNP into the ankle joint can effectively block ankle nerve conduction in rats.
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Baghban R, Afarid M, Soleymani J, Rahimi M. Were magnetic materials useful in cancer therapy? Biomed Pharmacother 2021; 144:112321. [PMID: 34656061 DOI: 10.1016/j.biopha.2021.112321] [Citation(s) in RCA: 10] [Impact Index Per Article: 2.5] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 08/21/2021] [Revised: 09/29/2021] [Accepted: 10/08/2021] [Indexed: 12/11/2022] Open
Abstract
Cancer is one of the major challenges fronting the biomedical basic researches in our time. The study and development of effective therapeutic strategies for cancer therapy are vital. Among the many probable core constituents of nanoparticles, magnetite-based nanoparticles have been widely studied for cancer therapy owing to their inherent magnetic features, multifunctional design, biodegradable and biocompatible properties. Magnetic nanoparticles have been also designed for utilizing as contrast enhancer agents for magnetic resonance imaging, drug delivery systems, and most recently as a therapeutic element in inducing cellular death in tumor ablation therapies. This review aimed to provide an overview of the various applications of magnetic nanoparticles and recent achievements in developing these advanced materials for cancer therapy.
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Affiliation(s)
- Roghayyeh Baghban
- Poostchi Ophthalmology Research Center, Department of Ophthalmology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran; Student Research Committee, Shiraz University of Medical Sciences, Shiraz, Iran
| | - Mehrdad Afarid
- Poostchi Ophthalmology Research Center, Department of Ophthalmology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran
| | - Jafar Soleymani
- Pharmaceutical Analysis Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
| | - Mahdi Rahimi
- Lodz University of Technology, Institute of Polymer and Dye Technology, Stefanowskiego 16, 90-537 Lodz, Poland.
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Ma X, Lee C, Zhang T, Cai J, Wang H, Jiang F, Wu Z, Xie J, Jiang G, Li Z. Image-guided selection of Gd@C-dots as sensitizers to improve radiotherapy of non-small cell lung cancer. J Nanobiotechnology 2021; 19:284. [PMID: 34551763 PMCID: PMC8456633 DOI: 10.1186/s12951-021-01018-9] [Citation(s) in RCA: 16] [Impact Index Per Article: 4.0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Grants] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 06/24/2021] [Accepted: 08/29/2021] [Indexed: 01/12/2023] Open
Abstract
BACKGROUND Recently, gadolinium-intercalated carbon dots (Gd@C-dots) have demonstrated potential advantages over traditional high-Z nanoparticles (HZNPs) as radiosensitizers due to their high stability, minimal metal leakage, and remarkable efficacy. RESULTS In this work, two Gd@C-dots formulations were fabricated which bore carboxylic acid (CA-Gd@C-dots) or amino group (pPD-Gd@C-dots), respectively, on the carbon shell. While it is critical to develop innovative nanomateirals for cancer therapy, determining their tumor accumulation and retention is equally important. Therefore, in vivo positron emission tomography (PET) was performed, which found that 64Cu-labeled pPD-Gd@C-dots demonstrated significantly improved tumor retention (up to 48 h post injection) compared with CA-Gd@C-dots. Indeed, cell uptake of 64Cu-pPD-Gd@C-dots reached close to 60% of total dose compared with ~ 5% of 64Cu-CA-Gd@C-dots. pPD-Gd@C-dots was therefore further evaluated as a new radiosensitizer for non-small cell lung cancer treatment. While single dose radiation plus intratumorally injected pPD-Gd@C-dots did lead to improved tumor suppression, the inhibition effect was further improved with two doses of radiation. The persistent retention of pPD-Gd@C-dots in tumor region eliminates the need of reinjecting radiosensitizer for the second radiation. CONCLUSIONS PET offers a simple and straightforward way to study nanoparticle retention in vivo, and the selected pPD-Gd@C-dots hold great potential as an effective radiosensitizer.
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Affiliation(s)
- Xiaofen Ma
- Department of Nuclear Medicine, Guangdong Second Provincial General Hospital, 466 Xingang Middle Road, Haizhu District, Guangdong Province, 510317, Guangzhou City, People's Republic of China
- Department of Radiology, Biomedical Research Imaging Center, and Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, 125 Mason Farm Road, Chapel Hill, NC, 27599, USA
| | - Chaebin Lee
- Department of Chemistry, University of Georgia, 140 Cedar Street, Athens, GA, 30602, USA
| | - Tao Zhang
- Department of Radiology, Biomedical Research Imaging Center, and Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, 125 Mason Farm Road, Chapel Hill, NC, 27599, USA
| | - Jinghua Cai
- Department of Radiology, Biomedical Research Imaging Center, and Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, 125 Mason Farm Road, Chapel Hill, NC, 27599, USA
| | - Hui Wang
- Department of Radiology, Biomedical Research Imaging Center, and Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, 125 Mason Farm Road, Chapel Hill, NC, 27599, USA
| | - Fangchao Jiang
- Department of Chemistry, University of Georgia, 140 Cedar Street, Athens, GA, 30602, USA
| | - Zhanhong Wu
- Department of Radiology, Biomedical Research Imaging Center, and Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, 125 Mason Farm Road, Chapel Hill, NC, 27599, USA
| | - Jin Xie
- Department of Chemistry, University of Georgia, 140 Cedar Street, Athens, GA, 30602, USA.
| | - Guihua Jiang
- Department of Nuclear Medicine, Guangdong Second Provincial General Hospital, 466 Xingang Middle Road, Haizhu District, Guangdong Province, 510317, Guangzhou City, People's Republic of China.
| | - Zibo Li
- Department of Radiology, Biomedical Research Imaging Center, and Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, 125 Mason Farm Road, Chapel Hill, NC, 27599, USA.
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Gong T, Cheng R, Wang X, Li J, Liang W, Wei Z, Shuang S, Wang Y, Guo R. Supramolecular-interaction-mediated aggregation of anticarcinogens on triformyl cholic acid-functionalized Fe 3O 4 nanoparticles and their dual-targeting treatment for liver cancer. NEW J CHEM 2021. [DOI: 10.1039/d1nj00248a] [Citation(s) in RCA: 2] [Impact Index Per Article: 0.5] [Reference Citation Analysis] [Abstract] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 02/02/2023]
Abstract
Herein, triformyl cholic acid-modified Fe3O4 nanoparticles (TCA-MNPs) were first constructed and developed as a novel drug carrier, possessing a high loading capacity, and the synergistic targeted therapy of hepatoma cells in vitro and in vivo.
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Affiliation(s)
- Tao Gong
- Department of Biochemistry and Molecular Biology
- Shanxi Medical University
- Taiyuan 030001
- China
| | - Rina Cheng
- Department of Biochemistry and Molecular Biology
- Shanxi Medical University
- Taiyuan 030001
- China
| | - Xiaoyu Wang
- Department of Biochemistry and Molecular Biology
- Shanxi Medical University
- Taiyuan 030001
- China
| | - Jing Li
- Department of Chemistry
- Institute of Environmental Science
- Shanxi University
- Taiyuan 030006
- P. R. China
| | - Wenting Liang
- Department of Chemistry
- Institute of Environmental Science
- Shanxi University
- Taiyuan 030006
- P. R. China
| | - Zhihong Wei
- Department of Chemistry
- Institute of Environmental Science
- Shanxi University
- Taiyuan 030006
- P. R. China
| | - Shaomin Shuang
- Department of Chemistry
- Institute of Environmental Science
- Shanxi University
- Taiyuan 030006
- P. R. China
| | - Yuyao Wang
- Department of Biochemistry and Molecular Biology
- Shanxi Medical University
- Taiyuan 030001
- China
| | - Rui Guo
- Department of Biochemistry and Molecular Biology
- Shanxi Medical University
- Taiyuan 030001
- China
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Li Y, Zhou Y, Yue X, Dai Z. Cyanine Conjugate-Based Biomedical Imaging Probes. Adv Healthc Mater 2020; 9:e2001327. [PMID: 33000915 DOI: 10.1002/adhm.202001327] [Citation(s) in RCA: 43] [Impact Index Per Article: 8.6] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 07/28/2020] [Revised: 09/11/2020] [Indexed: 12/12/2022]
Abstract
Cyanine is a class of fluorescent dye with meritorious fluorescence properties and has motivated numerous researchers to explore its imaging capabilities by miscellaneous structural modification and functionalization strategies. The covalent conjugation with other functional molecules represents a distinctive design strategy and has shown immense potential in both basic and clinical research. This review article summarizes recent achievements in cyanine conjugate-based probes for biomedical imaging. Particular attention is paid to the conjugation with targeting warheads and other contrast agents for targeted fluorescence imaging and multimodal imaging, respectively. Additionally, their clinical potential in cancer diagnostics is highlighted and some concurrent impediments for clinical translation are discussed.
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Affiliation(s)
- Yang Li
- Department of Biomedical Engineering College of Engineering Peking University Beijing 100871 China
| | - Yiming Zhou
- Department of Biomedical Engineering College of Engineering Peking University Beijing 100871 China
| | - Xiuli Yue
- School of Environment Harbin Institute of Technology Harbin 150090 China
| | - Zhifei Dai
- Department of Biomedical Engineering College of Engineering Peking University Beijing 100871 China
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Fu S, Cai Z, Liu L, Yang L, Jin R, Lu Z, Ai H. Controlled aggregation of amphiphilic aggregation‐induced emission polycation and superparamagnetic iron oxide nanoparticles as fluorescence/magnetic resonance imaging probes. J Appl Polym Sci 2020. [DOI: 10.1002/app.48760] [Citation(s) in RCA: 2] [Impact Index Per Article: 0.4] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 12/15/2022]
Affiliation(s)
- Shengxiang Fu
- National Engineering Research Center for BiomaterialsSichuan University Chengdu China
| | - Zhongyuan Cai
- National Engineering Research Center for BiomaterialsSichuan University Chengdu China
| | - Li Liu
- National Engineering Research Center for BiomaterialsSichuan University Chengdu China
| | - Li Yang
- National Engineering Research Center for BiomaterialsSichuan University Chengdu China
| | - Rongrong Jin
- National Engineering Research Center for BiomaterialsSichuan University Chengdu China
| | - Zhiyun Lu
- Key Laboratory of Green Chemistry and Technology (Ministry of Education), College of ChemistrySichuan University Chengdu China
| | - Hua Ai
- National Engineering Research Center for BiomaterialsSichuan University Chengdu China
- Department of Radiology, West China HospitalSichuan University Chengdu China
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Municoy S, Álvarez Echazú MI, Antezana PE, Galdopórpora JM, Olivetti C, Mebert AM, Foglia ML, Tuttolomondo MV, Alvarez GS, Hardy JG, Desimone MF. Stimuli-Responsive Materials for Tissue Engineering and Drug Delivery. Int J Mol Sci 2020; 21:E4724. [PMID: 32630690 PMCID: PMC7369929 DOI: 10.3390/ijms21134724] [Citation(s) in RCA: 107] [Impact Index Per Article: 21.4] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Grants] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 06/04/2020] [Revised: 06/28/2020] [Accepted: 06/30/2020] [Indexed: 02/07/2023] Open
Abstract
Smart or stimuli-responsive materials are an emerging class of materials used for tissue engineering and drug delivery. A variety of stimuli (including temperature, pH, redox-state, light, and magnet fields) are being investigated for their potential to change a material's properties, interactions, structure, and/or dimensions. The specificity of stimuli response, and ability to respond to endogenous cues inherently present in living systems provide possibilities to develop novel tissue engineering and drug delivery strategies (for example materials composed of stimuli responsive polymers that self-assemble or undergo phase transitions or morphology transformations). Herein, smart materials as controlled drug release vehicles for tissue engineering are described, highlighting their potential for the delivery of precise quantities of drugs at specific locations and times promoting the controlled repair or remodeling of tissues.
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Affiliation(s)
- Sofia Municoy
- Universidad de Buenos Aires, Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Instituto de la Química y Metabolismo del Fármaco (IQUIMEFA), Facultad de Farmacia y Bioquímica Junín 956, Piso 3° (1113), Buenos Aires 1113, Argentina; (S.M.); (M.I.Á.E.); (P.E.A.); (J.M.G.); (C.O.); (A.M.M.); (M.L.F.); (M.V.T.); (G.S.A.)
| | - María I. Álvarez Echazú
- Universidad de Buenos Aires, Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Instituto de la Química y Metabolismo del Fármaco (IQUIMEFA), Facultad de Farmacia y Bioquímica Junín 956, Piso 3° (1113), Buenos Aires 1113, Argentina; (S.M.); (M.I.Á.E.); (P.E.A.); (J.M.G.); (C.O.); (A.M.M.); (M.L.F.); (M.V.T.); (G.S.A.)
| | - Pablo E. Antezana
- Universidad de Buenos Aires, Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Instituto de la Química y Metabolismo del Fármaco (IQUIMEFA), Facultad de Farmacia y Bioquímica Junín 956, Piso 3° (1113), Buenos Aires 1113, Argentina; (S.M.); (M.I.Á.E.); (P.E.A.); (J.M.G.); (C.O.); (A.M.M.); (M.L.F.); (M.V.T.); (G.S.A.)
| | - Juan M. Galdopórpora
- Universidad de Buenos Aires, Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Instituto de la Química y Metabolismo del Fármaco (IQUIMEFA), Facultad de Farmacia y Bioquímica Junín 956, Piso 3° (1113), Buenos Aires 1113, Argentina; (S.M.); (M.I.Á.E.); (P.E.A.); (J.M.G.); (C.O.); (A.M.M.); (M.L.F.); (M.V.T.); (G.S.A.)
| | - Christian Olivetti
- Universidad de Buenos Aires, Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Instituto de la Química y Metabolismo del Fármaco (IQUIMEFA), Facultad de Farmacia y Bioquímica Junín 956, Piso 3° (1113), Buenos Aires 1113, Argentina; (S.M.); (M.I.Á.E.); (P.E.A.); (J.M.G.); (C.O.); (A.M.M.); (M.L.F.); (M.V.T.); (G.S.A.)
| | - Andrea M. Mebert
- Universidad de Buenos Aires, Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Instituto de la Química y Metabolismo del Fármaco (IQUIMEFA), Facultad de Farmacia y Bioquímica Junín 956, Piso 3° (1113), Buenos Aires 1113, Argentina; (S.M.); (M.I.Á.E.); (P.E.A.); (J.M.G.); (C.O.); (A.M.M.); (M.L.F.); (M.V.T.); (G.S.A.)
| | - María L. Foglia
- Universidad de Buenos Aires, Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Instituto de la Química y Metabolismo del Fármaco (IQUIMEFA), Facultad de Farmacia y Bioquímica Junín 956, Piso 3° (1113), Buenos Aires 1113, Argentina; (S.M.); (M.I.Á.E.); (P.E.A.); (J.M.G.); (C.O.); (A.M.M.); (M.L.F.); (M.V.T.); (G.S.A.)
| | - María V. Tuttolomondo
- Universidad de Buenos Aires, Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Instituto de la Química y Metabolismo del Fármaco (IQUIMEFA), Facultad de Farmacia y Bioquímica Junín 956, Piso 3° (1113), Buenos Aires 1113, Argentina; (S.M.); (M.I.Á.E.); (P.E.A.); (J.M.G.); (C.O.); (A.M.M.); (M.L.F.); (M.V.T.); (G.S.A.)
| | - Gisela S. Alvarez
- Universidad de Buenos Aires, Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Instituto de la Química y Metabolismo del Fármaco (IQUIMEFA), Facultad de Farmacia y Bioquímica Junín 956, Piso 3° (1113), Buenos Aires 1113, Argentina; (S.M.); (M.I.Á.E.); (P.E.A.); (J.M.G.); (C.O.); (A.M.M.); (M.L.F.); (M.V.T.); (G.S.A.)
| | - John G. Hardy
- Department of Chemistry, Faraday Building, Lancaster University, Lancaster, Lancashire LA1 4YB, UK
- Materials Science Institute, Faraday Building, Lancaster University, Lancaster, Lancashire LA1 4YB, UK
| | - Martin F. Desimone
- Universidad de Buenos Aires, Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Instituto de la Química y Metabolismo del Fármaco (IQUIMEFA), Facultad de Farmacia y Bioquímica Junín 956, Piso 3° (1113), Buenos Aires 1113, Argentina; (S.M.); (M.I.Á.E.); (P.E.A.); (J.M.G.); (C.O.); (A.M.M.); (M.L.F.); (M.V.T.); (G.S.A.)
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Biegger P, Ladd ME, Komljenovic D. Multifunctional Magnetic Resonance Imaging Probes. Recent Results Cancer Res 2020; 216:189-226. [PMID: 32594388 DOI: 10.1007/978-3-030-42618-7_6] [Citation(s) in RCA: 1] [Impact Index Per Article: 0.2] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 02/08/2023]
Abstract
Magnetic resonance imaging is characterized by high spatial resolution and unsurpassed soft tissue discrimination. Development and characterization of both intrinsic and extrinsic magnetic resonance (MR) imaging probes in the last decade has further strengthened the pivotal role MR imaging holds in the assessment of cancer in preclinical and translational settings. Sophisticated chemical modifications of a variety of nanoparticulate probes hold the potential to deliver valuable multifunctional tools applicable in diagnostics and/or treatment in human oncology. MR imaging suffers from a lack of sensitivity achievable by, e.g., nuclear medicine imaging methods. Advantages of including additional functionality/functionalities in a probe suitable for MR imaging are thus numerous, comprising the addition of fundamentally different imaging information (diagnostics), drug delivery (therapy), or the combination of both (theranostics). In recent years, we have witnessed a plethora of preclinical multimodal or multifunctional imaging probes being published mainly as proof-of-principle studies, yet only a handful are readily applicable in clinical settings. This chapter summarizes recent innovations in the development of multifunctional MR imaging probes and discusses the suitability of these probes for clinical transfer.
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Affiliation(s)
- Philipp Biegger
- Medical Physics in Radiology, German Cancer Research Center (DKFZ), Heidelberg, Germany
| | - Mark E Ladd
- Medical Physics in Radiology, German Cancer Research Center (DKFZ), Heidelberg, Germany.,Faculty of Medicine, University of Heidelberg, Heidelberg, Germany.,Faculty of Physics and Astronomy, University of Heidelberg, Heidelberg, Germany
| | - Dorde Komljenovic
- Medical Physics in Radiology, German Cancer Research Center (DKFZ), Heidelberg, Germany.
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15
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Wang Y, Shi L, Jin Y, Sun S, Gao P, Wei Y, Wang Z, Jiang Y. Surface-initiated polymerization for the preparation of magnetic polymer composites. Polym Chem 2020. [DOI: 10.1039/c9py01744e] [Citation(s) in RCA: 6] [Impact Index Per Article: 1.2] [Reference Citation Analysis] [Abstract] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 12/17/2022]
Abstract
A surface-initiated polymerization method is shown for the preparation of magnetic polymer composites with the good encapsulation of magnetic materials.
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Affiliation(s)
- Yang Wang
- School of Chemistry and Chemical Engineering
- Jiangsu Province Hi-Tech Key Laboratory for Biomedical Research
- Southeast University
- Nanjing 211189
- P.R. China
| | - Lin Shi
- School of Chemistry and Chemical Engineering
- Jiangsu Province Hi-Tech Key Laboratory for Biomedical Research
- Southeast University
- Nanjing 211189
- P.R. China
| | - Yajin Jin
- School of Chemistry and Chemical Engineering
- Jiangsu Province Hi-Tech Key Laboratory for Biomedical Research
- Southeast University
- Nanjing 211189
- P.R. China
| | - Si Sun
- School of Chemistry and Chemical Engineering
- Jiangsu Province Hi-Tech Key Laboratory for Biomedical Research
- Southeast University
- Nanjing 211189
- P.R. China
| | - Pengcheng Gao
- School of Chemistry and Chemical Engineering
- Jiangsu Province Hi-Tech Key Laboratory for Biomedical Research
- Southeast University
- Nanjing 211189
- P.R. China
| | - Yingying Wei
- School of Chemistry and Chemical Engineering
- Jiangsu Province Hi-Tech Key Laboratory for Biomedical Research
- Southeast University
- Nanjing 211189
- P.R. China
| | - Zhifei Wang
- School of Chemistry and Chemical Engineering
- Jiangsu Province Hi-Tech Key Laboratory for Biomedical Research
- Southeast University
- Nanjing 211189
- P.R. China
| | - Yong Jiang
- School of Chemistry and Chemical Engineering
- Jiangsu Province Hi-Tech Key Laboratory for Biomedical Research
- Southeast University
- Nanjing 211189
- P.R. China
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Abstract
In recent years, various reports related to sensing application research have suggested that combining the synergistic impacts of optical, electrical or magnetic properties in a single technique can lead to a new multitasking platform. Owing to their unique features of the magnetic moment, biocompatibility, ease of surface modification, chemical stability, high surface area, high mass transference, magnetic nanoparticles have found a wide range of applications in various fields, especially in sensing systems. The present review is comprehensive information about magnetic nanoparticles utilized in the optical sensing platform, broadly categorized into four types: surface plasmon resonance (SPR), surface-enhanced Raman spectroscopy (SERS), fluorescence spectroscopy and near-infrared spectroscopy and imaging (NIRS) that are commonly used in various (bio) analytical applications. The review also includes some conclusions on the state of the art in this field and future aspects.
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Wang XC, Zhou SX, Ding L, Zhao YH, Min SX, Dong B, Song B. Controllable Emission via Tuning the Size of Fluorescent Nano-probes Formed by Polymeric Amphiphiles. CHINESE JOURNAL OF POLYMER SCIENCE 2019. [DOI: 10.1007/s10118-019-2256-6] [Citation(s) in RCA: 4] [Impact Index Per Article: 0.7] [Reference Citation Analysis] [Track Full Text] [Subscribe] [Scholar Register] [Indexed: 11/28/2022]
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18
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Multari C, Miola M, Laviano F, Gerbaldo R, Pezzotti G, Debellis D, Verné E. Magnetoplasmonic nanoparticles for photothermal therapy. NANOTECHNOLOGY 2019; 30:255705. [PMID: 30790778 DOI: 10.1088/1361-6528/ab08f7] [Citation(s) in RCA: 9] [Impact Index Per Article: 1.5] [Reference Citation Analysis] [Abstract] [MESH Headings] [Track Full Text] [Subscribe] [Scholar Register] [Indexed: 06/09/2023]
Abstract
In recent decades the applications of nanotechnology in the biomedical field have attracted a lot of attention. Magnetic and gold nanoparticles (MNPs and GNPs) are now of interest as selective tools for tumour treatment, due to their unique properties and biocompatibility. In this paper, superparamagnetic iron oxide nanoparticles (MNPs) decorated with gold nanoparticles (GNPs) have been prepared by means of an innovative synthesis process using tannic acid as the reducing agent. The as-obtained nanoplatforms were characterized in terms of size, morphology, structure, composition, magnetic response and plasmonic properties. The results revealed that hybrid nanoplatforms (magnetoplasmonic nanoparticles, MPNPs) composed of a magnetic core and an external GNP decoration, acting in synergy, have been developed. Biological tests were also performed on both healthy cells and cancer cells exposed to different nanoparticle concentrations, upon laser irradiation. GNPs grafted onto the surface of MNPs revealed the ability to convert the received light into thermal energy, which was selective in its detrimental effect on cancer cells.
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Affiliation(s)
- C Multari
- Department of Applied Science and Technology, Politecnico di Torino, Turin, Italy
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19
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Olson MT, Ly QP, Mohs AM. Fluorescence Guidance in Surgical Oncology: Challenges, Opportunities, and Translation. Mol Imaging Biol 2019; 21:200-218. [PMID: 29942988 PMCID: PMC6724738 DOI: 10.1007/s11307-018-1239-2] [Citation(s) in RCA: 42] [Impact Index Per Article: 7.0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Grants] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 12/12/2022]
Abstract
Surgical resection continues to function as the primary treatment option for most solid tumors. However, the detection of cancerous tissue remains predominantly subjective and reliant on the expertise of the surgeon. Surgery that is guided by fluorescence imaging has shown clinical relevance as a new approach to detecting the primary tumor, tumor margins, and metastatic lymph nodes. It is a technique to reduce recurrence and increase the possibility of a curative resection. While significant progress has been made in developing this emerging technology as a tool to assist the surgeon, further improvements are still necessary. Refining imaging agents and tumor targeting strategies to be a precise and reliable surgical strategy is essential in order to translate this technology into patient care settings. This review seeks to provide a comprehensive update on the most recent progress of fluorescence-guided surgery and its translation into the clinic. By highlighting the current status and recent developments of fluorescence image-guided surgery in the field of surgical oncology, we aim to offer insight into the challenges and opportunities that require further investigation.
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Affiliation(s)
- Madeline T Olson
- Eppley Institute for Research in Cancer and Allied Diseases, University of Nebraska Medical Center, Omaha, NE, 68198, USA
- Fred and Pamela Buffet Cancer Center, University of Nebraska Medical Center, Omaha, NE, 68198, USA
| | - Quan P Ly
- Fred and Pamela Buffet Cancer Center, University of Nebraska Medical Center, Omaha, NE, 68198, USA
- Department of Surgery, University of Nebraska Medical Center, Omaha, NE, 68198, USA
| | - Aaron M Mohs
- Fred and Pamela Buffet Cancer Center, University of Nebraska Medical Center, Omaha, NE, 68198, USA.
- Department of Pharmaceutical Sciences, University of Nebraska Medical Center, 5-12315 Scott Research Tower, Omaha, NE, 68198, USA.
- Department of Biochemistry and Molecular Biology, University of Nebraska Medical Center, Omaha, NE, 68198, USA.
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20
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Ostroverkhov P, Semkina A, Naumenko V, Plotnikova E, Melnikov P, Abakumova T, Yakubovskaya R, Mironov A, Vodopyanov S, Abakumov A, Majouga A, Grin M, Chekhonin V, Abakumov M. Synthesis and characterization of bacteriochlorin loaded magnetic nanoparticles (MNP) for personalized MRI guided photosensitizers delivery to tumor. J Colloid Interface Sci 2019; 537:132-141. [DOI: 10.1016/j.jcis.2018.10.087] [Citation(s) in RCA: 23] [Impact Index Per Article: 3.8] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 06/23/2018] [Revised: 10/16/2018] [Accepted: 10/27/2018] [Indexed: 12/27/2022]
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21
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da Silva HR, Mamani JB, Nucci MP, Nucci LP, Kondo AT, Fantacini DMC, de Souza LEB, Picanço-Castro V, Covas DT, Kutner JM, de Oliveira FA, Hamerschlak N, Gamarra LF. Triple-modal imaging of stem-cells labeled with multimodal nanoparticles, applied in a stroke model. World J Stem Cells 2019; 11:100-123. [PMID: 30842808 PMCID: PMC6397806 DOI: 10.4252/wjsc.v11.i2.100] [Citation(s) in RCA: 13] [Impact Index Per Article: 2.2] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Journal Information] [Submit a Manuscript] [Subscribe] [Scholar Register] [Received: 10/20/2018] [Revised: 12/05/2018] [Accepted: 12/17/2018] [Indexed: 02/06/2023] Open
Abstract
BACKGROUND Mesenchymal stem cells (MSCs) have been widely tested for their therapeutic efficacy in the ischemic brain and have been shown to provide several benefits. A major obstacle to the clinical translation of these therapies has been the inability to noninvasively monitor the best route, cell doses, and collateral effects while ensuring the survival and effective biological functioning of the transplanted stem cells. Technological advances in multimodal imaging have allowed in vivo monitoring of the biodistribution and viability of transplanted stem cells due to a combination of imaging technologies associated with multimodal nanoparticles (MNPs) using new labels and covers to achieve low toxicity and longtime residence in cells.
AIM To evaluate the sensitivity of triple-modal imaging of stem cells labeled with MNPs and applied in a stroke model.
METHODS After the isolation and immunophenotypic characterization of human bone marrow MSCs (hBM-MSCs), our team carried out lentiviral transduction of these cells for the evaluation of bioluminescent images (BLIs) in vitro and in vivo. In addition, MNPs that were previously characterized (regarding hydrodynamic size, zeta potential, and optical properties), and were used to label these cells, analyze cell viability via the 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide assay and BLI analysis, and quantify the internalization process and iron load in different concentrations of MNPs via magnetic resonance imaging (MRI), near-infrared fluorescence (NIRF), and inductively coupled plasma-mass spectrometry (ICP-MS). In in vivo analyses, the same labeled cells were implanted in a sham group and a stroke group at different times and under different MNP concentrations (after 4 h or 6 d of cell implantation) to evaluate the sensitivity of triple-modal images.
RESULTS hBM-MSC collection and isolation after immunophenotypic characterization were demonstrated to be adequate in hBM samples. After transduction of these cells with luciferase (hBM-MSCLuc), we detected a maximum BLI intensity of 2.0 x 108 photons/s in samples of 106 hBM-MSCs. Analysis of the physicochemical characteristics of the MNPs showed an average hydrodynamic diameter of 38.2 ± 0.5 nm, zeta potential of 29.2 ± 1.9 mV and adequate colloidal stability without agglomeration over 18 h. The signal of iron load internalization in hBM-MSCLuc showed a close relationship with the corresponding MNP-labeling concentrations based on MRI, ICP-MS and NIRF. Under the highest MNP concentration, cellular viability showed a reduction of less than 10% compared to the control. Correlation analysis of the MNP load internalized into hBM-MSCLuc determined via the MRI, ICP-MS and NIRF techniques showed the same correlation coefficient of 0.99. Evaluation of the BLI, NIRF, and MRI signals in vivo and ex vivo after labeled hBM-MSCLuc were implanted into animals showed differences between different MNP concentrations and signals associated with different techniques (MRI and NIRF; 5 and 20 µg Fe/mL; P < 0.05) in the sham groups at 4 h as well as a time effect (4 h and 6 d; P < 0.001) and differences between the sham and stroke groups in all images signals (P < 0.001).
CONCLUSION This study highlighted the importance of quantifying MNPs internalized into cells and the efficacy of signal detection under the triple-image modality in a stroke model.
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Affiliation(s)
| | | | - Mariana Penteado Nucci
- LIM44, Hospital das Clinicas HCFMUSP, Faculdade de Medicina, Universidade de São Paulo, São Paulo 05403-010, Brazil
| | | | | | | | | | - Virginia Picanço-Castro
- Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, São Paulo 05403-010, Brazil
| | - Dimas Tadeu Covas
- Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, São Paulo 05403-010, Brazil
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22
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Mohammadi M, Rezaei Mokarram R, Hamishehkar H. Glutathione decorated gold-magnetic nanoparticles: efficient and recyclable catalyst for biotechnological and pharmaceutical applications. J Microencapsul 2019; 35:559-569. [DOI: 10.1080/02652048.2018.1554011] [Citation(s) in RCA: 5] [Impact Index Per Article: 0.8] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 12/22/2022]
Affiliation(s)
- Maryam Mohammadi
- Department of Food Science and Technology, Faculty of Agriculture, University of Tabriz, Tabriz, Iran
| | - Reza Rezaei Mokarram
- Department of Food Science and Technology, Faculty of Agriculture, University of Tabriz, Tabriz, Iran
| | - Hamed Hamishehkar
- Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
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23
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Duan M, Shapter JG, Qi W, Yang S, Gao G. Recent progress in magnetic nanoparticles: synthesis, properties, and applications. NANOTECHNOLOGY 2018; 29:452001. [PMID: 30142088 DOI: 10.1088/1361-6528/aadcec] [Citation(s) in RCA: 30] [Impact Index Per Article: 4.3] [Reference Citation Analysis] [Abstract] [Track Full Text] [Subscribe] [Scholar Register] [Indexed: 06/08/2023]
Abstract
The rapid development of advanced nanotechnology has continuously changed many aspects of society. One important nanostructured material, magnetic nanoparticles (NPs), has applications in many areas including clean energy, biology and engineering because of their special magnetic properties. The synthesis of magnetic nanomaterials with desired sizes and morphology has attracted great attention. Nanomaterials with different properties can be combined to construct multifunctional nanoplatforms through systematic surface engineering. The surface modification of magnetic NPs presents the opportunity for them to be used in many practical applications. Functionalized magnetic NPs have been successfully applied in catalysis, as thermoelectric materials, for drug delivery, as imaging agents in nuclear magnetic resonance and in biosensors. In this review, synthetic methods for magnetic NPs and some of their important properties are described. Then the latest progress of the application of magnetic NPs in energy and biology has been summarized and discussed. Finally, we discuss some issues that still need to be solved and the prospects for magnetic NPs.
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Affiliation(s)
- Meng Duan
- Institute of Nano Biomedicine and Engineering, Key Laboratory for Thin Film and Micro Fabrication of the Ministry of Education, Department of Instrument Science and Engineering, School of Electronic Information and Electrical Engineering, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai 200240, People's Republic of China
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Leng F, Liu F, Yang Y, Wu Y, Tian W. Strategies on Nanodiagnostics and Nanotherapies of the Three Common Cancers. NANOMATERIALS 2018; 8:nano8040202. [PMID: 29597315 PMCID: PMC5923532 DOI: 10.3390/nano8040202] [Citation(s) in RCA: 18] [Impact Index Per Article: 2.6] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Subscribe] [Scholar Register] [Received: 02/18/2018] [Revised: 03/18/2018] [Accepted: 03/23/2018] [Indexed: 02/07/2023]
Abstract
The emergence of nanomedicine has enriched the knowledge and strategies of treating diseases, and especially some incurable diseases, such as cancers, acquired immune deficiency syndrome (AIDS), and neurodegenerative diseases. The application of nanoparticles in medicine is in the core of nanomedicine. Nanoparticles can be used in drug delivery for improving the uptake of poorly soluble drugs, targeted delivery to a specific site, and drug bioavailability. Early diagnosis of and targeted therapies for cancers can significantly improve patients' quality of life and extend patients' lives. The advantages of nanoparticles have given them a progressively important role in the nanodiagnosis and nanotherapy of common cancers. To provide a reference for the further application of nanoparticles, this review focuses on the recent development and application of nanoparticles in the early diagnosis and treatment of the three common cancers (lung cancer, liver cancer, and breast cancer) by using quantum dots, magnetic nanoparticles, and gold nanoparticles.
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Affiliation(s)
- Fan Leng
- Department of Biomedical Engineering, School of Basic Medical Sciences, Wuhan University, Wuhan 430071, China.
| | - Fang Liu
- Department of Biomedical Engineering, School of Basic Medical Sciences, Wuhan University, Wuhan 430071, China.
| | - Yongtao Yang
- Department of Biomedical Engineering, School of Basic Medical Sciences, Wuhan University, Wuhan 430071, China.
| | - Yu Wu
- Department of Biomedical Engineering, School of Basic Medical Sciences, Wuhan University, Wuhan 430071, China.
| | - Weiqun Tian
- Department of Biomedical Engineering, School of Basic Medical Sciences, Wuhan University, Wuhan 430071, China.
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