Variations of mitochondrial D-loop region plus downstream gene 12S rRNA-tRNAphe and gastric carcinomas

Table 1 Variant frequencies of D-loop region, 12S rRNA and tRNA^phe in gastric cancers and para-cancerous tissues

Type	n	Variation frequency vs controls
		D-loop	12S rRNA	tRNAphe
Papillary adenocarcinoma	4	3/2	0/0	-c
Well differentiated adenocarcinoma	4	1/1	0/1a	-c
Moderately differentiated adenocarcinoma	4	2/1	2/1	-c
Poorly differentiated adenocarcinoma	4	3/2	3/2b	1/0
Signet-ring cell carcinoma	3	2/1	1/0	-c
Mucous adenocarcinoma	3	2/2	2/2b	1/0
Total	22	13/9	8/6	2/0

A: np 772 A-T transition was only found in normal tissues. B: One of variations in para-cancerous tissue was not the same as cancerous tissue. C: No variation was found.

Table 2 Instabilities and variations of mitochondrial D-loop and 12s rRNA-tRNA^phe in 22 patients with gastric carcinoma

Map locus	Map position	Description	Variant position	Variation
HV2	57-372	Hypervariable segment 2	73	A-G
OHR	110-441	H-strand origin	143	G-A
			146	T-C
			152	T-C
			204	T-C
CSB1	213-235	Conserved sequence block I	235	A-G
TFX	233-260	mtTF1 binding site	235	A-G
			263	A-G
TFY	276-303	mtTF1 binding site	-	-b
CSB2	299-315	Conserved sequence block II	310	CTCC
			312	T	CCCCC
HPR	317-321	Replication primer	318(317)	C-Ta
			321(320)	C-Ta
CSB3	346-363	Conserved sequence block III	340	C-T
			343	C-Ta
			345	C-Ta
MT4H	371-379	mt4 H-strand control element	-	-b
MT3H	384-391	mt3 H-strand control element	-	-b
LSP	392-445	L-strand promoter	-	-b
TFL	418-445	mtTF1 binding site	481	C-T
			483	C-A
			489	T-C
			514	CACA
TFH	523-550	mtTF1 binding site	523	AC-dela
			527	C-Ta
HSP1	545-567	Major H-strand promoter	-	-b
TF	577-647	tRNA phenylalanine	611	G insa
			641	A-T
HSP2	645-645	Minor H-strand promoter
RNR1	648-1601	12S rRNA	652	G-insa
			709	G-A
			716	T-dela
				T-G*
			728	C-T
			745	A-Ga
			757	A-T
			760	A-Ta
			772	A-Ta
			1106	T-C
			1160	A-G
			1226	C-T
HV1	16024-16383	Hypervariable segment 1	16182	AA-CCa
			16189	T-C
TAS	16157-16172	Termination-associated sequence	-	-b
MT5	16194-16208	Control element	16194	A-G

Notes: A: Variations not found previously, B: Relatively stable and no variation of base, del: Deletion, “ins”: Insertion, CC or CA: Insertion characteristics of instability.