Oral immunization of animals with transgenic cherry tomatillo expressing HBsAg

Table 1 Expression of HBsAg in different organs and differ-ent plants of same line

Plant No.	Organ	Total soluble protein (mg/g fresh weight)	Content of HBsAg (ng/g fresh weight)	HBsAg/TSP (1/1000000)
1#	Leaf	31.6	300	9
	Stem	5.8	15	3
	Fruit	0.7	7	10
2#	Leaf	30.0	240	8
	Stem	5.0	9	2
	Fruit	0.6	6	10
3#	Leaf	27.3	210	8
	Stem	3.6	7	2
	Fruit	0.6	6	10
4#	Leaf	11.3	90	8
	Stem	2.6	5	2
CK	Leaf	28.4	-	-
	Stem	4.7	-	-
	Fruit	0.6	-	-

Note: 1#, 2#, 3#, 4# were different plants of same transgenic cherry tomatillo line; 1#, 2#, 3# were transferred to soil in the greenhouse; 4# was still in tissue culture; CK was a non-transformed plant growing in the greenhouse.

Table 2 Expressed target protein

Line No.	Total soluble protein (mg/g fresh weight)	Content of HBsAg (ng/g fresh weight)	HBsAg/TSP (1/1000000)
1#	16.0	45	3
2#	15.5	24	2
3#	12.6	21	2
4#	13.1	90	7
CK	13.7	-

Note: 1#, 2#, 3# and 4# were four different lines of transgenic cherry tomatillo with normal phenotype, and these materials were all leaves of transgenic plants in the condition of tissue culture; CK was leaf of a non-transformed tomatillo in tissue culture.