Pleckstrin homology domain of G protein-coupled receptor kinase-2 binds to PKC and affects the activity of PKC kinase

Table 1 First three β sheets of PH domains of GRK₂ and GRK₃ are compared with other PH domain known or predicted to bind to PKC

PH domain (source)	β1	β2	β3
Btk	ESIFLKT--SQQKKKTSPLS--NFKKRLFLLT-VH-KLSYYEYDF
β-spectrin	MEGFLNRKH-EWEAHNKKASSR-SWHNVYCVIN-NQ--EMGFYKDAK
Dynamin	RKGWLTI---NNIGIMKGGS----KEYWFVLT--AE--NLSWYKDDE
AFAP, Nt PH domain	ICAFLLRKK-RFGQ----------WTKLLCVIK-EN--KLLCYKSSK
Consensus	1* +*	++*****	+L 1p
GRK2	MHGYMSKM--GNPFLTQ-----WQRRYFYLF---PN--RLEWRGEGEA
GRK3	MHGYMLKL--GNPFLTQ-----WQRRYFYLF---PN--RLEWRGEGES

Consensus sequence is shown and symbols used are as in the paper of Baisden et al. 1 indicates aromatic residues, + indi-cates positively charged residues, *indicates hydrophobic residues, indicates polar residues. The sequences of GRK₂ and GRK₃ from Zheng et al[18].