Anti-Helicobacter pylori immunoglobulin G (IgG) and IgA antibody responses and the value of clinical presentations in diagnosis of Helicobacter pylori infection in patients with precancerous lesions

Table 1 The detected positive rates of H. pylori at different stages of CG

H. pylori	Mild CAG	Severe CAG	Mild IM	Severe IM	DYS	Total
Number	76	22	22	58	31	209
Positive %	39 (51.3%)	15 (68.2%)a	16 (72.7%)	49 (84.5%)b	21 (67.7%)	140
Negative %	37 (48.7%)	7 (31.8%)	6 (27.3%)	9 (15.5%)	10 (32.3%)	69

CAG, chronic atrophic gastritis; IM, intestinal metaplasia; DYS, dysplasia.

^aP < 0.05,

^bP < 0.01, mild IM vs severe IM; mild CAG vs. severe CAG.

Table 2 The detected positive rates of H. pylori for CG patients in relation to gender and age

H. pylori	Males	Females	70-77 years old	60-69 years old	50-59 years old	42-49 years old
Number	103	106	16	47	66	80
Positive (%)	64 (62.1%)	76 (71.7%)	10 (62.5%)	32 (68.1%)	39 (59.1%)	59 (73.8%)
Negative (%)	39 (37.9%)	30 (28.3%)	6 (37.5%)	15 (31.9%)	27 (40.9%)	21 (26.2%)

Table 3 Comparison of serum anti-H. pylori IgG and IgA levels (the optical density value) at different stages of CG

	Mild CAG		Severe CAG		Mild IM		Severe IM		DYS
	H. pylori (+)	H. pylori (-)	H. pylori (+)	H. pylori (-)	H. pylori (+)	H. pylori (-)	H. pylori (+)	H. pylori (-)	H. pylori (+)	H. pylori (-)
IgG (1)	2.86 ± 1.8b	0.38 ± 0.27	4.48 ± 2.14bc	0.53 ± 0.27	3.86 ± 1.87b	0.14 ± 0.07	3.1 ± 2.34a	0.55 ± 0.29d	2.74 ± 1.67b	0.23 ± 0.17
IgG (2)	3.26 ± 2.16b	0.46 ± 0.24	4.52 ± 3.27a	0.54 ± 0.29	4.44 ± 2.56b	0.3 ± 0.18	3.5 ± 1.98b	0.54 ± 0.29	3.33 ± 1.87b	0.72 ± 0.97
IgA (1)	0.85 ± 0.7b	0.24 ± 0.22	0.99 ± 0.49b	0.21 ± 0.16	0.83 ± 0.39b	0.1 ± 0.06	0.93 ± 0.79	0.33 ± 0.16d	0.94 ± 0.89b	0.13 ± 0.13
IgA (2)	0.96 ± 0.83b	0.25 ± 0.21	1.08 ± 0.59a	0.27 ± 0.16	0.99 ± 0.72a	0.12 ± 0.07	0.89 ± 0.63a	0.32 ± 0.19d	0.78 ± 0.6b	0.15 ± 0.13

CAG, chronic atrophic gastritis; IM, intestinal metaplasia; DYS, dysplasia. (1) The first detection. (2) The second detection.

^aP < 0.01,

^bP < 0.001, H. pylori (+) vs. H. pylori (-).

^cP < 0.05, H. pylori (+) mild CAG vs. H. pylori (+) severe CAG;

^dP < 0.05, H. pylori (-) mild IM vs. H. pylori (-) severe IM.

Table 4 Diagnostic accuracy of H. pylori infection determined by different assemblages of symptoms

Attributes	Assemblages of symptoms	Accuracy (%)
		H. pylori (+)	H. pylori (-)
Pathogenic factors	Smoking, alcohol, limbs, hand and foot (alla)	70.7%	42.3%
and general status
Pathogenic factors,	Smoking, alcohol, dietary regularity, limbs, and tongue quality (alla)	74.3%	39.1%
general status and tongue
Pathogenic factors and	Smoking, alcohol, limbs, hand and foot, distending fullness in the stomach,	51.4%	52.2%
digestive symptoms	stomachache, distending fullness in the abdomen, and nausea (alla)
Digestive symptoms	Appetite, distending fullness in the stomach, stomachache, distending fullness	71.4%	26.1%
	in the abdomen, pain in the hypochondrium, pain in the abdomen, singultus,
	nausea, vomit, acid regurgitation and epigastric upset, heartburn, and stool (alla)
Digestive symptoms	Distending fullness in the stomach, stomachache, pain in the abdomen,	72.1%	26.1%
and tongue	nausea, and fur of tongue (alla)
General status	Taste, swollen, head, limbs, chest, hand and foot, spirit and sleep,	50.7%	40.6%
	emotion, complexion (alla)
Total symptoms	35 itemsb	65.7%	40.6%

^aP < 0.05-0.001, the difference between two classes tested by the ANOVA analysis.

^bP < 0.05-0.001, except dietary bias, dietary regularity, urine, oropharynx, eye, ear, physique, tongue quality, and tongue body.