Analysis of gene expression profile induced by EMP-1 in esophageal cancer cells using cDNA Microarray

Table 1 Genes whose expression was altered more than 2.0-fold

Genes up-regulated by EMP-1
Coordinate	Gene Name	Ratio	Category	Sub Category
A3i	Cyclin-dependent kinase 4 inhibitor 2D	2.04	Cell division	Cell-cycle regulators
A7d	Cytokeratin 1	2.26	Cell structure	Intermediate Filament Proteins
B3i	Caspase 9	4.11	Cell division	Cell-apoptosis
B7b	Chromatin assembly factor 1 p48 subunit	2.93	Cell division	Chromosome structure
B7j	Abelson-related gene	4.49	Tumor Suppressors	Oncogenes & Suppressors
C2e	Chromatin assembly factor 1 p48 subunit	2.80	Cell division	Apoptosis-Associated Proteins
C2j	Abelson-related gene	2.47	Cell signaling	Intracellular transducers
C3e	Chromatin assembly factor 1 p48 subunit	5.15	Cell division	DNA synthesis/replication
C4b	Abelson-related gene	3.09	Cell Adhesion	Extracellular Communication Proteins
C4k	Chromatin assembly factor 1 p48 subunit	4.13	Cell signaling	Intracellular Transducers
C7m	Abelson-related gene	5.47	Cell division	Death Receptors
D3e	Chromatin assembly factor 1 p48 subunit	2.36	Cell structure	Extracellular Matrix Proteins
D4j	Abelson-related gene	2.27	Cell Adhesion	Matrix Adhesion Receptors
D4k	Chromatin assembly factor 1 p48 subunit	2.45	Cell Adhesion	Cell-Cell Adhesion Receptors
D5g	Ezrin; cytovillin 2; villin 2 (VIL2)	3.06	Tumor Suppressors	Oncogenes & Tumor Suppressors
D6l	Caveolin 1	3.29	Metabolism	GTP/GDP Exchangers
E1h	Matrix metalloproteinase 11 (MMP11)	4.64	Cell structure	Extracellular matrix Proteins
E1m	Matrix metalloproteinase 16 (MMP16)	3.06	Protein modification	Metalloproteinases
E2b	Tissue inhibitor of metalloproteinase 1	3.82	Protein modification	Protease Inhibitors
E2h	Tissue-type plasminogen activator	4.39	Protein modification	Serine Proteases
E5b	RHO GDP-dissociation inihibitor 1	2.42	Metabolism	GTP/GDP Exchangers
E5h	Cadherin 5 (CDH5);	2.64	Cell Adhesion	Cell-Cell Adhesion Receptors
F1h	Bone morphogenetic protein 2A (BMP2A)	12.45	Cell communication	Cytokines
F3j	Early growth response protein 1	16.06	RNA synthesis	Transcription Activators & Repressors
F4l	Interleukin 6 (IL6)	4.28	Cell communication	Interleukins & Interferons
F5e	Interleukin 13 (IL13)	2.62	Cell communication	Interleukins & Interferons
F5k	Interferon gamma (IFN-gamma; IFNG)	6.04	Cell communication	Interleukins & Interferons
F5l	Leukocyte interferon-inducible peptide	2.73	Unclassified	Functionally Unclassified Proteins
A1j	Cell division cycle 25 homolog A	0.35	Cell division	Cell-cycle regulators
2m	Cyclin D2 (CCND2)	0.34	Cell division	Cell-cycle regulators
A2n	G1/S-specific cyclin D3 (CCND3)	0.30	Cell division	Cell-cycle regulators
A3k	Polo-like kinase (PLK)	0.47	Cell division	Cell Cycle-Regulating Kinases
D1m	Collagen VI alpha 2 subunit (COL6A2)	0.32	Cell communication	Extracellular Matrix Proteins
D2b	Collagen XI alpha 1 subunit (COL11A1)	0.17	Cell communication	Extracellular Matrix Proteins
D7e	Vascular endothelial growth factor C	0.11	Cell communication	Growth Factors,

Table 2 Summary of genes regulated by EMP-1

Major category	Sub category	Up-regulated	Down-regulated
Cell division	General	1
	DNA synthesis/replication	1
	Apoptosis	2
	Cell cycle	1	4
	Chromosome structure	1
	Total	6	4
Cell signaling	Cell adhesion
or cell	Channels/transport protein
communication	Effectors/modulators
and adhesion	Extracellular Communication	1
	Hormones/growth factors	4	1
	Intracellular transducers	2
	Protein modification	3
	Receptors	3	2
	Total	13	3
Cell structure/	General
motility	Cytoskeletal	3
	Extracellular matrix Protein	2
	Microtubule-associated proteins/motors	1
	Total	5
Cell/organism	General
defense	Homeostasis
	Immunology	1
	Total	1
Gene/protein	RNA synthesis	1
expression	protein synthesis
	Total	1
Metabolism	General
	Amino acid
	Cofactors
	Energy/TCA cycle	2
	Lipid
	Nucleotide
	Protein modification
	Sugar/glycolysis
	Transport
	Total	2
	Unclassified	1
	Total	1