Copyright
©The Author(s) 2003.
World J Gastroenterol. Dec 15, 2003; 9(12): 2720-2725
Published online Dec 15, 2003. doi: 10.3748/wjg.v9.i12.2720
Published online Dec 15, 2003. doi: 10.3748/wjg.v9.i12.2720
Strains and plasmids | Characteristics | Source (reference) |
Strains | ||
DH5α | endA1 hsdR17(rk-mk+) supE44 thi-1 recA1 gyrA(Nalr) | Our lab |
RelA1 △ lacIZYA-argF) U169 deoR (φ80dlac △(lacZ)M15) | ||
S17-1λpir | thi-1 thr leu tonA lacY supE recA::RP4-2-Tc::Mu, Smr, λpir | Guzman CA |
MC1061 | hsdR2 mcrB araD139 △ (ara ABC-leu)7679 △ lacX74 galU galK rpsL thi, Smr | Our lab |
2457T | S. flexneri 2a wild type, Nalr | Maurelli AT |
2457T05 | a derivative strain of 2457T, contained araBp-gam-bet-exo, Nalr | This study |
2457T028D | △ alkA, derivative strain of 2457T, Nalr, Kmr | This study |
Plasmids | ||
pMD18-T | a derivative constructed from pUC18, Apr | TaKaRa |
pXL275 | a mobile and suicide plasmid, ori R6K, mob RK2, sacBR, Cmr | Rui et al[17] |
pKD46 | oriR101, repA101(ts), araBp-gam-bet-exo, Apr | CGSCa |
pXLkd46 | pXL275 derivative, inserted a fragment containing araBp-gam-bet-exo, Cmr | This study |
pMDKm05 | pMD18-T derivative, inserted Kmr gene, Kmr, Apr | Our lab |
pMD028 | pMD18-T derivative, inserted alkA gene, Apr | This study |
pMD028pKm | pMD18-T derivative, inserted a cassette (5’alkA end-Kmr-3’alkA end), Kmr, Apr | This study |
Bacteria | Gene product | Gene function | Identities (%) |
Escherichia coli K12 | 3-methyl-adenine DNA glycosylase II | DNA - replication, repair, restriction/modification | 97% |
Escherichia coli O157:H7 | 3-methyl-adenine DNA glycosylase II | Macromolecule synthesis, modification: DNA - replication, repair, restriction/modification | 97% |
Salmonella typhimurium | 3-methyl-adenine DNA glycosylase II | Unknown | 74% |
Ralstonia solanacearum | putative transcription regulator protein | Miscellaneous; not classified regulator | 38% |
Pseudomonas aeruginosa | DNA-3-methyladenine glycosidase II | Unknown | 37% |
Xanthomonas axonopodis | DNA methylation and regulatory protein | Unknown | 35% |
Mycobacterium bovis | Methylated-DNA—protein-cysteine methyltransferase | Adaptative response | 34% |
Mycobacterium tuberculosis | alkA protein | Unknown | 34% |
Vibrio cholerae | ada regulatory protein | Unknown | 32% |
Bacillus anthracis | DNA-3-methyladenine glycosidase | Unknown | 32% |
Archaeoglobus fulgidus | 3-methyladenine DNA glycosylase | DNA repair at suboptimal and maybe even mesophilic temperatures | 30% |
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Citation: Shi ZX, Wang HL, Hu K, Feng EL, Yao X, Su GF, Huang PT, Huang LY. Identification of
alkA gene related to virulence ofShigella flexneri 2a by mutational analysis. World J Gastroenterol 2003; 9(12): 2720-2725 - URL: https://www.wjgnet.com/1007-9327/full/v9/i12/2720.htm
- DOI: https://dx.doi.org/10.3748/wjg.v9.i12.2720