Purification and characterization of 33.5 kDa vesicular protein in human bile

Table 1 Amino acid composition of 33.5 kDa vesicular protein

Amino acid	nmol/total protein	No. of residues/mol protein
Asp/Asn	6.761	19
Thr	4.488	13
Ser	1.589	5
Glu/Gln	10.434	30
Gly	2.242	6
Ala	2.864	8
Val	2.501	7
Ile	3.226	9
Leu	4.782	14
Tyr	1.937	6
Phe	2.966	8
Lys	4.777	14
His	0.840	2
Arg	2.645	8
Pro	1.411	4
NH2	11.297	32
Total	64.76	153

Table 2 Effect of 33.5 kDa vesicular protein on activity indices of cholesterol crystallization (100 μg/mL)

	It	Ig	Ic	P value
Purified 33.5 kDa protein	0.57	1.52	1.63	< 0.05a
+ N-deglycosylation	1.08	1.01	0.98	< 0.05b
+ O-deglycosylation	0.58	1.61	1.54	< 0.05a
+ Proteolysis	1.12	0.87	0.99	< 0.05b

a: compared with control, b: compared with 33.5 kDa vesicular protein group.