Copyright
©The Author(s) 2003.
World J Gastroenterol. Jan 15, 2003; 9(1): 89-93
Published online Jan 15, 2003. doi: 10.3748/wjg.v9.i1.89
Published online Jan 15, 2003. doi: 10.3748/wjg.v9.i1.89
Table 1 Primer sequences for the coding region of the LPTS gene
| Exon | Nucleotide sequences | PCR conditions | Product size (bp) |
| 1 | Forward: 5’-CGTGCTCGAGGAGCGAGTCG-3’ | ||
| Reverse: 5’-ACCCGGCATCTTCACCAACG-3’ | 52 °C SD | 241 | |
| 2 | Forward: 5’-TCCATTGCTGATGATAATGC-3’ | ||
| Reverse: 5’-CTTCCAGTCTCCTAAGAAGG-3’ | 50 °C SD | 230 | |
| 3 | Forward: 5’-TGAGAGGAATGTTCTAACTC-3’ | ||
| Reverse: 5’-AGCCAAYYAYGCAAAGACAC-3’ | 50 °C SD | 178 | |
| 4 | Forward: 5’-AACTACAGGCTTACCTCTCG-3’ | ||
| Reverse: 5’-AACATATTTGCATTGAGAAC-3’ | 55 °C SD | 196 | |
| 5 | Forward: 5’-CAAGACTATCCACTGTTAGG-3’ | ||
| Reverse:5’-GGACAAACACGTAGATTTCAATAAC-3’ | 52 °C Standard | 220 | |
| 6 | Forward: 5’-GCTGCATAGTTCATGTCTGC-3’ | ||
| Reverse: 5’-CACAGGTGAAAATCAGACAG-3’ | 194 | ||
| 7.1 | Forward: 5’-CTGCCTTTTAACTCTTCTGC-3’ | ||
| Reverse: 5’-GGCTGGAGGTAACTTTCCAC-3’ | 50 °C SD | 247 | |
| 7.2 | Forward: 5’-GGCCACAGGTAAAGATGTGG-3’ | ||
| Reverse: 5’-CAGGCGGCTGCACATGGTCC-3’ | 55 °C Standard | 200 | |
| 7.3 | Forward: 5’-GCCTCTGCTCAGGATGCAGG-3’ | ||
| Reverse: 5’-GCCCCGGCTGGGAAGGATTC-3’ | 50 °C SD | 191 |
Table 2 The alterations of LPTS-S and LPTS-L in tumor cell lines
| Cell line | Cell type | Mutation | Exon | Codon | Predicted effect |
| CCLP1 | Cholangiocarcinoma | C161T | Exon 2 | gct→gtt | Ala→Val |
| HepG2 | HCC | A701A, G(LPTS-S) | Exon 7 | Outside ORF | / |
| A778A, G(LPTS-L) | aca→gca | Thr→Ala | |||
| HepT1 | HCC | C183T | Exon 2 | gcc→gct | Ala |
| MEK | HCC | A701A, G(LPTS-S) | Exon 7 | Outside ORF | / |
| A778A, G(LPTS-L) | aca→gca | Thr→Ala | |||
| SNU182 | HCC | A701A, G(LPTS-S) | Exon 7 | Outside ORF | / |
| A778A, G(LPTS-L) | aca→gca | Thr→Ala | |||
| Li7A | HCC | A803T (LPTS-S) | Exon 7 | Outside ORF | / |
| A880T (LPTS-L) | agc→tgc | Ser→Cys | |||
| Li21 | HCC | C183T | Exon 2 | gcc→gct | Ala |
| A803T (LPTS-S) | Exon 7 | Outside ORF | / | ||
| A880T (LPTS-L) | agc→tgc | Ser→Cys | |||
| MZCHA1 | HCC | A803T (LPTS-S) | Exon 7 | Outside ORF | / |
| A880T (LPTS-L) | agc→tgc | Ser→Cys | |||
| PCI-SG231 | HCC | G111C | Exon 1 | Before ATG | / |
| A803T (LPTS-S) | Exon 7 | Outside ORF | / | ||
| A880T (LPTS-L) | agc→tgc | Ser→Cys | |||
| PLCPRF5 | HCC | A497G | Exon5 | tat→tgt | Tyr→Cys |
| IGR-OV1 | Ovarian tumor | A701A, G (LPTS-S) | Exon 7 | Outside ORF | / |
| A778A, G (LPTS-L) | aca→gca | Thr→Ala | |||
| SW579 | Head and neck tumor | C183C, T | Exon 2 | gcc→gct | Ala |
- Citation: Liao C, Zhao MJ, Zhao J, Song H, Pineau P, Marchio A, Dejean A, Tiollais P, Wang HY, Li TP. Mutation analysis of novel human liver-related putative tumor suppressor gene in hepatocellular carcinoma. World J Gastroenterol 2003; 9(1): 89-93
- URL: https://www.wjgnet.com/1007-9327/full/v9/i1/89.htm
- DOI: https://dx.doi.org/10.3748/wjg.v9.i1.89