Effect of apoptosis on gastric adenocarcinoma cell line SGC-7901 induced by cis-9, trans-11-conjugated linoleic acid

Table 1 Cell cycle analysis of SGC-7901 cells induced by c9, t11-CLA at 24 h and 48 h (mean ± SD, n = 4)

c9,t11-CLA(μm)	Apoptosis		G0/G1		G2/M		S
	24 h	48 h	24 h	48 h	24 h	48 h	24 h	48 h
0	0.55 ± 0.09	0.69 ± 0.21	53.13 ± 3.44	58.57 ± 0.90	11.13 ± 2.75	12.64 ± 1.18	35.74 ± 2.04	28.80 ± 0.89
25	1.27 ± 0.73	3.89 ± 2.12	53.00 ± 8.35	56.81 ± 1.09	15.55 ± 1.11	15.95 ± 0.36	31.40 ± 9.23	26.52 ± 1.46
50	4.12 ± 0.55a	8.18 ± 1.55b	56.58 ± 0.87	56.32 ± 0.78	14.55 ± 3.45	15.17 ± 0.61	28.87 ± 3.32	28.51 ± 1.32
100	7.95 ± 0.31b	12.33 ± 1.53b	58.35 ± 2.44	61.18 ± 4.94	13.12 ± 1.50	14.49 ± 3.10	28.52 ± 0.96	25.08 ± 2.85
200	12.79 ± 3.12b	14.75 ± 5.97b	60.67 ± 4.28a	63.82 ± 7.84a	12.95 ± 4.48	13.35 ± 3.91	26.38 ± 0.92b	22.82 ± 4.63b

^aP < 0.05,

^bP < 0.01 vs negative control

Table 2 Positive rates of bcl-2, Fas and c-myc on SGC-7901 cells treated with c9, t11-CLA (%)

C9,t11-CLA(μmol/L)	24 h			48 h
	bcl-2	Fas	c-myc	bcl-2	Fas	c-myc
0	6.80	0.60	5.50	8.00	0.45	5.30
25	4.30	0	4.85	8.05	0.85	4.75
50	2.50b	0	4.20a	3.80b	2.75b	3.70a
100	1.45b	1.95b	3.80a	0.30b	4.10b	1.35b
200	0.15b	2.75b	2.20b	0	5.95b	0.30b

^aP < 0.05,

^bP < 0.01 vs negative control.