Prevalence of hepatitis G virus infection and homology of different viral strains in Southern China

Table 1 Samples for sequencing

Sample	Sex	Age (yrs)	Origin	Diagnosis	Regions for sequencing
GD1	F	58	Guangdong	non A-E hepatitis	5’UTR
GD3027	M	47	Guangdong	hepatitis B	5’UTR, NS5A
GD3040	M	76	Guangdong	hepatitis B	5’UTR
GD3064	F	20	Guangdong	non A-E hepatitis	5’UTR
GDCA	M	26	Guangdong	hepatocellular carcinoma	5’UTR
YN1	M	26	Yunnan	intravenous drug user	5’UTR
YN2	M	38	Yunnan	intravenous drug user	5’UTR
YN3	M	45	Yunnan	intravenous drug user	5’UTR
HKC9	F	42	Hong Kong	hepatitis C	5’UTR
HKC16	M	38	Hong Kong	hepatitis C	5’UTR
HK8	M	56	Hong Kong	recipient of bone marrow	5’UTR
HK9	M	42	Hong Kong	recipient of bone marrow	5’UTR
HK10	M	38	Hong Kong	recipient of bone marrow	5’UTR
HK11	M	42	Hong Kong	recipient of bone marrow	5’UTR
HK12	M	42	Hong Kong	recipient of bone marrow	5’UTR
HK24	F	42	Hong Kong	recipient of bone marrow	5’UTR
HK80	M	40	Hong Kong	recipient of bone marrow	5’UTR
HK108	M	18	Hong Kong	recipient of bone marrow	5’UTR
HK116	M	42	Hong Kong	recipient of bone marrow	5’UTR
HK120	M	40	Hong Kong	recipient of bone marrow	5’UTR
A132	M	20	Hong Kong	blood donor	NS5A
A711	M	25	Hong Kong	blood donor	NS5A

HK9 and HK12 were derived from the same patient after a 3-month interval; HK11 and HK116 were also from a patient after a 3-month interval

Table 2 Primers used in RT-PCR

Primer	Polarity	Position	Nucleotide sequence
G1	+	117-136	5’ ATGCGTGATGACAGGGTTGG 3’
G2	-	451-471	5’ TAGGTGGCCCCATGCATTTCC 3’
G3	+	161-180	5’ GGTAGCCACTATAGGTGGGT 3’
G4	-	379-398	5’ CACTGGTCCTTGTCAACTCG 3’
33	+	6672-6697	5’ GTTGAATTCGCGATGGAGCGCTACAC 3’
34	-	7267-7292	5’ CTGGGATCCGTATCATGTATGGTTCT 3’
35	+	6573-6592	5’ TCGATTGCTGTAGCTGAGCC 3’
36	-	7327-7346	5’ GGTAAGTTCATTGCCCACCA 3’

The numbering is identical to that of the PNF2161 strain of HGV[1].

Table 3 Prevalence of HGV infection from different groups in Southern China

Group	cases	HGV RNA Positive cases(%)
Bone marrow transplantation	108	45(41.67)
Haemodialysis	92	13(14.13)
Intravenous drug users	84	15(17.86)
Hepatocellular carcinoma	161	22(13.66)
Hepatitis B	263	19(7.22)
Hepatitis C	55	7(12.73)
Hepatitis B + Hepatitis A	16	1(6.25)
Hepatitis B + Hepatitis C	6	1(16.67)
Hepatitis B + Hepatitis E	26	3(11.54)
Hepatitis E	29	2(6.90)
Non A-E hepatitis	83	21(25.30)
Blood donors	506	13(2.57)
General population	562	5(0.89)
Total	1991