Effects of Chinese Jianpi herbs on cell apoptosis and related gene expression in human gastric cancer grafted onto nude mice

Table 1 Sequences of primers for amplifed cDNA of the p53, bcl-2 and β-actin

Primers		Sequences	Combining sites	Amplifiers
p53	Sense	5'-GGAGGTTGTGAGGCGCTGC-3	645 bp-663 bp	311 bp
	Antisense	5'-CACGCACCTCAAAGCTGTTC-3	936 bp-955 bp
bcl-2	Sense	5'-CAGCTGCACCTGACGCCCTT-3'	1810 bp-1829 bp	191 bp
	Antisense	5'-GCCTCCGTTATCCTGGATCC-3'	2021 bp-2040 bp
β-actin	Sense	5'-AGCGGGAAATCGTGCGTGAC-3	665 bp-674 bp	471 bp
	Antisense	5'-ACTCCTGCTTGCTGATCCACATC-3	1103 bp-1125 bp

Table 2 Chinese Jianpi herbs-induced effects on gastric cancer cell SGC-7901 (¯x ± s)

Treatment	Tumor weight/g (n)	Tumor size/mm3 (n)	Percentage of control/%	Apoptotic index (AI)/%		ki-67/%
				TUNEL (n)	FACScan (n)
Sijunzi Decoction	0.66 ± 0.16 (9)a	371.81 ± 52.51 (9)a	34.33	16.24 ± 3.21 (9)b	11.38 ± 6.46 (9)a	7.95 ± 2.54 (9)c
SRRS	0.54 ± 0.23 (9)b	322.66 ± 126.14 (9)b	46.53	13.18 ± 3.05 (8)a	11.58 ± 5.71 (8)a	8.43 ± 2.22 (8)b
Control	1.01 ± 0.32 (10)	603.61 ± 263.39 (10)		2.63 ± 1.03 (10)	7.51 ± 1.32 (10)	10.37 ± 4.91 (10)

^aP < 0.05,

^bP < 0.01,

^cP = 0.070 vs control t test.

Table 3 Chinese Jianpi herbs-induced genetic effects (¯x ± s)

Treatment	p53 (exons 5-8)	bcl-2
Sijunzi Decoction	0.36 ± 0.27 (8)a	0.41 ± 0.15 (8)c
SRRS	0.19 ± 0.18 (9)b	0.33 ± 0.23 (9)b
Control	0.69 ± 0.20 (9)	0.81 ± 0.40 (9)

^aP < 0.05,

^bP < 0.01,

^cP = 0.071 vs control t test.