Copyright
©The Author(s) 2002.
World J Gastroenterol. Aug 15, 2002; 8(4): 619-623
Published online Aug 15, 2002. doi: 10.3748/wjg.v8.i4.619
Published online Aug 15, 2002. doi: 10.3748/wjg.v8.i4.619
Table 1 ratio of positive recombinant phage antibody clone and soluble ScFv
| Recombinant phage antibodies | soluble ScFv | |||
| Ratio of positive clone (%) | Ratio of competitive clone (%) | Ratio of positive clone (%) | Ratio of competitive clone (%) | |
| MC3 ScFv | 60 | 8 | 30 | 8 |
| MC5 ScFv | 24 | 6 | 18 | 4 |
| MGD1 ScFv | 30 | 4 | 10 | 4 |
Table 2 Concordance for MC3-ScFv-19 and it's parental mAb specificity to cells
| Cell lines | MC3-ScFv 19 | MC3 Ab |
| AGS | 1.403 ± 0.132 | 0.929 ± 0.187 |
| SW480 | 0.921 ± 0.201 | 0.647 ± 0.132 |
| SGC7901 | 0.257 ± 0.045 | 0.259 ± 0.078 |
| Fibroblast | 0.325 ± 0.106 | 0.297 ± 0.056 |
- Citation: Nie YZ, He FT, Li ZK, Wu KC, Cao YX, Chen BJ, Fan DM. Identification of tumor associated single-chain Fv by panning and screening antibody phage library using tumor cells. World J Gastroenterol 2002; 8(4): 619-623
- URL: https://www.wjgnet.com/1007-9327/full/v8/i4/619.htm
- DOI: https://dx.doi.org/10.3748/wjg.v8.i4.619