Copyright
©The Author(s) 2002.
World J Gastroenterol. Apr 15, 2002; 8(2): 282-287
Published online Apr 15, 2002. doi: 10.3748/wjg.v8.i2.282
Published online Apr 15, 2002. doi: 10.3748/wjg.v8.i2.282
Table 1 Relationship between coated McAb and the sensitivity of SPASE
Coated McAb (mg•L⁻¹) | Serum titer | Negative control | ||||||
1:1 | 1:10 | 1:20 | 1:50 | 1:100 | 1:200 | 1:500 | ||
12.5 | +++ | ++ | + | - | - | - | - | - |
25 | ++++ | +++ | ++ | + | - | - | - | - |
50 | ++++ | ++++ | ++++ | ++++ | ++ | + | - | - |
100 | ++++ | ++++ | ++++ | +++ | ++ | + | - | - |
200 | ++++ | +++ | +++ | ++ | + | - | - | - |
400 | +++ | ++ | ++ | ++ | + | - | - | - |
Table 2 Relation between the density of sensitized erythrocytes and the sensitivity of SPASE
Density of sensitized erythrocytes | Serum titer | Negative control | ||||||
1:1 | 1:10 | 1:20 | 1:50 | 1:100 | 1:200 | 1:500 | ||
1.5•L⁻¹ | +++ | ++ | ++ | - | - | - | - | - |
2.5•L⁻¹ | ++++ | ++++ | ++++ | +++ | ++ | + | - | - |
3.0•L⁻¹ | ++++ | ++++ | +++ | ++ | + | + | - | - |
6.0•L⁻¹ | +++ | +++ | ++ | + | - | - | - | - |
10.0•L⁻¹ | ++ | +++ | ++ | - | - | - | - | - |
Table 3 Repetitive experiment of samples
Well numbers | (¯x ± s) | CV (%) | |
In the same lot | 15 | 0.642 ± 0.0389 | 6.06 |
In different lot | 5 | 0.575 ± 0.044 | 7.65 |
Table 4 Results of detecting TIMP-1 and TIMP-2 in different sera
Clinical type | n | TIMP-1 | TIMP-2 | ||
Positive number | % | Positive number | % | ||
Acute hepatitis | 128 | 21 | 16.4 | 16 | 12.6 |
Chronic hepatitis | 174 | 59 | 33.9 | 48 | 27.6 |
Hepatic cirrhosis | 106 | 78 | 73.6 | 65 | 61.3 |
Table 5 Expressing of TIMP-1 and TIMP-2 in liver
Group | n | TIMP-1 | TIMP-2 | ||||||
- | + | ++ | +++ | - | + | ++ | +++ | ||
Normal group | 10 | 10 | 0 | 0 | 0 | 10 | 0 | 0 | 0 |
Hepatic cirrhosis | 40 | 0 | 8 | 4 | 28 | 0 | 8 | 10 | 22 |
Table 6 Expressing of TIMP-1 mRNA and TIMP-2 mRNA in liver of hepatic cirrhosis
Group | n | TIMP-1 | TIMP-2 | ||||||
+++ | ++ | + | - | +++ | ++ | + | - | ||
Hepatic cirrhosis | 40 | 32 | 6 | 2 | 0 | 22 | 16 | 2 | 0 |
Normal liver | 10 | 0 | 0 | 0 | 10 | 0 | 0 | 0 | 10 |
- Citation: Nie QH, Cheng YQ, Xie YM, Zhou YX, Bai XG, Cao YZ. Methodologic research on TIMP-1, TIMP-2 detection as a new diagnostic index for hepatic fibrosis and its significance. World J Gastroenterol 2002; 8(2): 282-287
- URL: https://www.wjgnet.com/1007-9327/full/v8/i2/282.htm
- DOI: https://dx.doi.org/10.3748/wjg.v8.i2.282