Effects of cryopreservation and phenylacetate on biological characters of adherent LAK cells from patients with hepatocellular carcinoma

Table 1 Proliferative capacity of A-LAK cells

Culture Days	Cells expansion folds
	A-LAK	NA-LAK	Regular LAK
1	1.35 ± 0.39	0.87 ± 0.21a	0.83 ± 0.09a
4	2.22 ± 0.85	1.09 ± 0.33a	0.99 ± 0.03a
7	4.42 ± 2.05	2.13 ± 0.83a	1.55 ± 0.14b
10	22.52 ± 10.47	8.49 ± 3.91a	3.25 ± 0.64b
14	96.79 ± 69.10	22.77 ± 13.20a	4.64 ± 0.91a

^aP < 0.05, vs compared with α-LAK cells of the same day;

^bP < 0.01, vs compared with α-LA cells of the same day

Table 2 Changes of phenotype of cryopteserved A-LAK cells

	Case No.	CD3	CD4	CD8	CD4/CD8	CD16	CD25
Before freezing	6	76.5 ± 10.3	41.4 ± 7.5	40.3 ± 5.3	1.03 ± 0.31	10.4 ± 3.1	52.3 ± 5.9
Day 0 after thawing	6	80.5 ± 11.4	35.9 ± 6.4	36.5 ± 4.9	1.07 ± 0.38	14.1 ± 4.3	49.7 ± 3.1
Day 3 after thawing	6	86.3 ± 8.9	33.1 ± 7.5	42.6 ± 6.0	0.78 ± 0.19	15.4 ± 2.7	46.5 ± 4.7