Construction and selection of the natural immune Fab antibody phage display library from patients with colorectal cancer

Table 1 Binding of Fab displayed by prime phage display librar ies with colorectal cancer antigens (A₄₉₀)

	Ag1	Ag2	Ag3	Lovo	Wash buffer
K1	1.285	1.496	1.332	0.326	0.105
K2	1.324	1.552	1.352	0.322	0.122
BC	0.115	0.105	0.110	0.092	0.087

Table 2 Binding activity of phage antibodies with antigens (A₄₉₀）

Phage antibodies	Ag1, Ag2 and Ag3	Lovo	Rat colon antigens	Wash buffer
Clone 1	1.236	1.042	0.121	0.096
Clone 2	1.117	0.873	0.110	0.100
Clone 3	1.450	1.329	0.182	0.121
Clone 4	1.106	0.912	0.106	0.087
Clone 5	1.345	1.024	0.090