Preparation of single chain variable fragment of MG7 mAb by phage display technology

Table 1 ELISA results of screening from enriched phage displayed antibody library

Screening	Number of positive clones (A- value)						Neg. ctrl
	1	2	3	4	5	6
First round	0.495	0.508	0.488	0.805	0.845	0.580	0.157
Second round	0.543	0.606	0.560	0.840	0.796	0.758	0.185

Table 2 ELISA results of the soluble MG₇ ScFv for binding with KATOIII cells

ELISA	Number of strong positive clones (A value)		Neg. ctrl
	1	2
First round	0.776	0.287	0.201
Second round	0.802	0.346	0.223