Inhibiting effect of antisense oligonucleotides phosphorthioate on gene expression of TIMP-1 in rat liver fibrosis

Table 1 TIMP-1, β-actin primer sections

Primer		Nucleic acid sections	Position（bp）
TIMP-1	Positive strand	5'-TTCGTGGGGACACCAGAAGTC-3'
	Antisence strand	5'-TATCTGGGACCGCAGGGACTG-3'	482
β-actin	Positive strand	5'-GGAGAAGATGACCCAGATCA-3'
	Antisence strand	5'-GATCTTCATGAGGTAGTCAG-3'	234

Table 2 Expression of TIMP-1 related antigen in the liver of rats

Group	n	TIMP-1
Normal group	10	59.8 ± 20.3
Experimental group	6	98.7 ± 25.7
Treatment group	6	396.1 ± 58.4
Model group	6	481.1 ± 61.0

Table 3 Gene expression of TIMP-1 in the liver of rats

Group	n	TIMP-1
Normal group	10	0.3 ± 0.1
Experimental group	6	0.6 ± 0.1
Treatment group	6	1.7 ± 0.4
Model group	6	1.9 ± 0.5

Table 4 Collagen I, III in the liver of rats

Group	n	Collagen I	Collagen III
Normal group	10	62 ± 17	100 ± 19
Experimental group	6	165 ± 47	349 ± 48
Treatment group	6	314 ± 65	516 ± 72
Model group	6	441 ± 87	699 ± 102

Table 5 mRNA expression of procollagen I, III in the liver of rats

Group	n	Procollagen I	Procollagen III
Normal group	10	245.6 ± 88.7	228.3 ± 57.5
Experimental group	6	917.9 ± 206.9	1050.9 ± 271.4
Treatment group	6	896.8 ± 198.1	977.8 ± 221.4
Model group	6	1217.3 ± 202.9	1484.5 ± 249.1

Table 6 The pathologic grading of hepatic fibrosis of experimental animal

Group	n	Pathologic grading of hepatic fibrosis
		0	I	II	III	IV	V	VI
Normal group	10	10	0	0	0	0	0	0
Experimental group	6	0	2	3	1	0	0	0
Treatment group	6	0	0	0	1	2	2	1
Model group	6	0	0	0	0	0	3	3