Multiple genetic alterations and behavior of cellular biology in gastric cancer and other gastric mucosal lesions: H. pylori infection, histological types and staging

Table 1 Expressions of p53, p16, Bcl-2, and COX-2 proteins in gastric mucosal lesions (n, %)

Lesion	n	p53	p16	Bcl-2	COX-2
GC	60	28 (46.7)a	23 (38.3)	41 (68.3)ba	45 (75.0)a
Dys	49	10 (20.4)	20 (40.8)	36 (73.5)ba	18 (36.7)
IM	78	1 (1.3)	43 (55.1)	33 (42.3)	21 (26.9)
CAG	56	0 (0)	39 (69.6)a	9 (16.1)	5 (8.9)
CSG	84	0 (0)	67 (79.8)b	7 (8.3)	0 (0)

p53:

^aP < 0.05 GC-Dys; p16:

^bP < 0.01 CSG-Dys or GC, ^aP < 0.05 CAG-Dys or GC; Bcl-2: ^bP < 0.01 Dys (GC)-CSG or CAG; ^aP < 0.05 Dys (GC)-IM; COX-2: ^aP < 0.05 GC-Dys (IM).

Table 2 Expression rates of p53, p16, Bcl-2, and COX-2 proteins in gastric mucosal lesions with H. pylori infection (n, %)

Protein	CSG (84)		CAG (56)		IM (78)		Dys (49)		GC (60)
	Hp+ (49)	Hp-(35)	Hp+ (31)	Hp-(25)	Hp+ (32)	Hp-(46)	Hp+ (21)	Hp-(28)	Hp+ (33)	Hp-(27)
p53	0	0	0	0	1	0	6 (28.6)	4 (14.3)	17 (515)	11 (40.7)
p16	36 (73.5)	31 (88.6)	17 (54.8)	22 (88.0)a	11 (34.4)	32 (69.6)a	5 (23.8)	15 (53.6)a	11 (33.3)	12 (44.4)
Bcl-2	4 (8.2)	3 (8.6)	6 (19.4)	3 (12.0)	22 (68.8)a	11 (23.9)	19 (90.5)a	17 (60.7)	25 (75.6)	16 (59.3)
COX-2	0	0	3 (9.7)	2 (8.0)	16 (50.0)a	5 (10.8)	13 (61.8)a	5 (17.8)	26 (78.7)	19 (70.4)

^aP < 0.05 p16: (CAG, IM or Dys) Hp+-Hp-; Bcl-2 or COX-2: (IM or Dys) Hp+-Hp-.

Table 3 The expression intensity of p53, p16, Bcl-2, and COX-2 proteins in gastric mucosal lesions with H. pylori infection (n)

Lesion	Hp	p53				p16				Bcl-2				COX-2
		-	+	++	+++	-	+	++	+++	-	+	++	+++	-	+	++	+++
CSG	+					13	17	13	6	45	3	1
	-					4	13	15	3	32	2	1
CAG	+					14b	12	5		25	2	4		28	3
	-					3	5	9	8	22	2	1		23	2
IM	+		1			21b	7	4		10a	4	10	8	16a	5	11
	-					14	4	15	13	35	6	5		41	4	1
Dys	+	15	4	2		16a	2	3		2a	2	11	6	8a	3	10
	-	24	2	2		13	2	8	5	11	9	5	3	23	3	2
GC	+	16	6	8	3	22	4	5	2	8	6	11	8	7	2	17	7
	-	16	4	6	1	15	5	6	1	11	5	9	2	8	4	12	3

^bP < 0.01 p16 (CAG or IM) Hp+-Hp-; ^aP < 0.05 p16 (Dys) Hp+-Hp-; Bcl-2 or COX-2 (IM or Dys) Hp+-Hp-.

Table 4 Comparisons of each parameter of CIA between H. pylori positive and H. pylori negative

parameters	Hp+ (33)	Hp-(33)
Ellipser (μm)	79 ± 23	58 ± 15b
Ellipseb (μm)	53 ± 14	40 ± 12a
Ratio1 (%)	22 ± 5	13 ± 4b
Ratio2 (%)	79 ± 17	53 ± 20b
Area1 (μm2)	748 ± 572	301 ± 202a
Area2 (μm2)	3050 ± 1661	1681 ± 1990a
Fshape	0.67 ± 0.09	0.71 ± 1.10

^aP < 0.05, Ellipseb, Area₁ or Area₂: Hp+-Hp-;

^bP < 0.01, Ellipser, Ratio₁ or Ratio₂: Hp+-Hp-.

Table 5 Expressions of p53, p16, Bcl-2, and COX-2 proteins in GC related to tumor staging and histological types (n, %)

Lesion	n	p53	p16	Bcl-2	COX-2
Type
Intestinal	24	14 (58.3)	9 (37.5)	18 (75.0)a	16 (66.7)
Diffuse	6	3 (30.0)	2 (33.3)	1 (16.7)	4 (66.7)
Stage of GC
Early	5	2 (40.0)	2 (40.0)	4 (80.0)	1 (20.0)
Advanced	25	15 (60.0)	9 (36.0)	15 (60.0)	19 (76.0)a
Grade of differentiation
High or middle	14	6 (42.9)	6 (42.9)	12 (85.7)	8 (57.1)
Low or not	10	8 (80.0)	3 (30.0)	7 (70.0)	8 (80.0)
Lymph node metastasis
No	4	2 (50.0)	2 (50.0)	3 (75.0)	1 (16.7)
Yes	26	15 (57.7)	9 (34.6)	16 (61.5)	19 (79.2)a

^aP < 0.05 Bcl-2: Intestinal-Diffuse; COX-2: Advanced-Early, Lymph node metastasis Yes-No.