Effect of Boschniakia rossica on expression of GST-P, p53 and p21ras proteins in early stage of chemical hepatocarcinogenesis and its anti-inflammatory activities in rats

doi:10.3748/wjg.v6.i6.812

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Dec 15, 2000 (publication date) through Feb 27, 2025

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Gastroenterol. Dec 15, 2000; 6(6): 812-818
Published online Dec 15, 2000. doi: 10.3748/wjg.v6.i6.812

Table 1 Effect of Boschniakia rossica on the expression of GST-P, p53 and p21 protein in early stages of rat chemical hepatocarcinogenesis

Groups	Treatment (8 wk)	n	GST-P positive (%)	No.of foci (No/cm²)^*	Area of foci (mm²/cm²)^*	p53 positive (%)	p21 positive (%)
A	Saline-BD-PH	10	Negative	0	0	Negative	Negative
B	DEN-AAF-PH	9	7 (77.8)	18.9 ± 1.54	0.27 ± 0.32	3 (33.3)	2 (22.2)
C	DEN-AAF-BR-PH	10	2 (20.0)^a	0.30 ± 0.67^b	0.03 ± 0.07^a	Negative	Negative

^aP < 0.05, vs B (χ² test); P < 0.05,

^bP < 0.01, vs B (t test).

^*Foci more than 0.1 nm in diameter were quantified; ^△Values ¯x ± s

Table 2 Effect of BR-extract on xylene-induced mouse ear edema

Groups	Dose (mg/kg)	Mouse (n)		BR-H₂O fraction (I)		BR-CH₂Cl₂ fraction (II)
Groups	Dose (mg/kg)	I	II	Edema degree (mg)	Inhibitory rate (%)	Edema degree (mg)	Inhibitory rate (%)
NS (A)	0.85%	10	20	19.1 ± 3.6^*		11.2 ± 4.0
Ind (B)	20	10	20	15.5 ± 1.8^a	18.8	8.8 ± 2.6^a	21.5
BR (C)	500	10	20	17.4 ± 3.1^b	8.9	8.3 ± 3.9^a	26.2
BR (D)	1000	10	20	13.6 ± 3.1^a	28.8	8.1 ± 3.7^a	27.4

^*¯x ± s,

^aP < 0.05;

^bP < 0.01, vs NS (t test); NS: normal saline; Ind: indomethacin; BR: Boschniakia rossica

Table 3 Effect of BR-extract on Croton oil-induced mouse ear edema

Groups	Dose (mg/kg)	Mouse (n)		BR-H₂O fraction (I)		BR-CH₂Cl₂ fraction (II)
Groups	Dose (mg/kg)	I	II	Edema degree (mg)	Inhibitory rate (%)	Edema degree (mg)	Inhibitory rate (%)
NS (A)	0.85%	10	12	8.6 ± 2.5^*		7.6 ± 3.3
Ind (B)	20	10	12	6.6 ± 4.0^a	23.3	5.0 ± 2.1^a	33.5
BR (C)	500	10	12	5.4 ± 3.4^b	45.3	4.9 ± 1.7^a	35.1
BR (D)	1000	10	12	4.7 ± 1.7^c	59.3	4.5 ± 2.8^a	40.0

^aP < 0.05;

^bP < 0.01;

^cP < 0.001; vs A (t test); NS: normal saline; Ind:indomethacin; BR: Boschniakia rossica

Table 4 Inhibitory effect of BR-H₂O extract on carrageenin-induced paw edema in rats (¯x ± s)

Groups	Dose (mg/kg)	Rats (n)	Swelling degree (inhibitory rate)
Groups	Dose (mg/kg)	Rats (n)	1 h	2 h	3 h	4 h	6 h	24 h
NS		9	39.5 ± 16.4	57.1 ± 19.3	63.6 ± 26.1	67.9 ± 22.6	67.5 ± 19.5	28.2 ± 13.0
Ind	20	9	25.9 ± 13.8	30.6 ± 19.2^a	32.6 ± 18.6^a	38.6 ± 22.1^a	42.2 ± 34.6	16.9 ± 12.3
			(34.2)	(46.5)	(47.2)	(43.1)	(37.5)	(39.9)
BR	500	9	30.6 ± 13.8	28.7 ± 8.3^b	39.4 ± 12.2^a	40.1 ± 14.2^a	39.5 ± 20.0^a	9.6 ± 8.5^b
			(22.7)	(49.8)	(38.0)	(36.2)	(41.5)	(65.8)
BR	1000	9	31.8 ± 15.7	26.3 ± 18.9^b	36.5 ± 19.9^a	37.0 ± 288^a	23.5 ± 16.4^c	6.8 ± 10.4^b
			(19.6)	(54.0)	(40.6)	(45.5)	(65.2)	(76.5)

^aP < 0.05;

^bP < 0.01;

^cP < 0.001, vs NS (t test)

Table 5 Effect of BR-H₂O extract on adjuvant arthitis in rats

Groups	Dose (mg/kg)	Rats (n)	Swelling degree (inhibitory rate)
Groups	Dose (mg/kg)	Rats (n)	18 h	3d	6d	9d	12d	15d	18d	21d	24d
NS		6	108.2 ± 43.8	74.7 ± 33.0	66.9 ± 47.9	58.0 ± 39.0	77.8 ± 39.6	77.1 ± 28.0	99.6 ± 34.9	78.6 ± 31.3	71.5 ± 28.7
			108.2 ± 43.8	74.7 ± 33.0	66.9 ± 47.9	58.0 ± 39.0	77.8 ± 39.6	77.1 ± 28.0	99.6 ± 34.9	78.6 ± 31.3	71.5 ± 28.7
BR	500	6	79.1 ± 22.3^b	33.9 ± 17.2^a	51.8 ± 25.0	52.6 ± 23.7	67.5 ± 21.1	75.7 ± 23.7	61.7 ± 19.0^a	53.4 ± 14.9^b	46.5 ± 17.2^b
			79.1 ± 22.3^b	33.9 ± 17.2^a	51.8 ± 25.0	52.6 ± 23.7	67.5 ± 21.1	75.7 ± 23.7	61.7 ± 19.0^a	53.4 ± 14.9^b	46.5 ± 17.2^b
			(26.9)	(54.7)	(22.6)	(9.3)	(13.2)	(1.8)	(38.1)	(32.1)	(35.0)

^aP < 0.05;

^bP < 0.01, vs NS (t test), NS: normal saline; Ind: indomethacin; BR:Boschniakia rossica

Table 6 Effect of BR-H₂O extract on hot scald-induced paw edema in rats

Groups	Dose (mg/kg)	Rats (n)	Swelling degree (inhibitory rate)
Groups	Dose (mg/kg)	Rats (n)	3 h	2 h	3 h	4 h	5 h	6 h	24 h
NS		8	67.2 ± 4.7	59.5 ± 3.9	74.2 ± 6.5	81.5 ± 23.7	77.7 ± 17.1	69.8 ± 19.1	63.7 ± 14.5
BR	500	8	40.4 ± 11.9^b	23.3 ± 10.1^b	33.5 ± 16.8^b	39.7 ± 10.5^a	24.8 ± 8.2^b	18.0 ± 16.1^b	23.4 ± 7.0^b
			(39.9)	(60.8)	(68.6)	(51.3)	(68.1)	(74.2)	(63.3)

^aP < 0.05;

^bP < 0.01, vs NS (t test) NS: normal saline; Ind: indomethacin; BR: Boschniakia rossica

Table 7 Effect of BR-H₂O extract on histamine-induced rat hind paw edema

Groups	Dose (mg/kg)	Rats (n)	Swelling degree (inhibitory rate)
Groups	Dose (mg/kg)	Rats (n)	30 min	3 h	2 h	3 h	4 h
NS		9	48.0 ± 11.1	34.8 ± 12.6	22.9 ± 8.1	18.3 ± 3.8	12.7 ± 5.9
BR	500	9	28.5 ± 7.5^a	18.4 ± 7.5^a	9.0 ± 6.5^a	9.6 ± 6.7^a	2.3 ± 3.9^b
			(36.2)	(31.1)	(31.7)	(36.8)	(71.3)

^aP < 0.05;

^bP < 0.01, vs NS (t test)

Table 8 Effect of BR-HO extract on proliferation of granuloma caused by cotton pellet in mice

Groups	Dose (mg/kg)	Mouse (n)	Weight of granuloma		Inhibitory rate (%)
Groups	Dose (mg/kg)	Mouse (n)	Wet w. (mg)	Dry w. (mg)	Wet w. (mg)	Dry w. (mg)
NS (A)		10	615.2 ± 119.1	152.3 ± 54.8
BR (C)	250	10	507.0 ± 41.1^a	103.7 ± 14.4^a	17.6	31.9
BR (D)	500	10	463.5 ± 49.6^b	101.0 ± 15.1^a	24.7	33.7

^aP < 0.05;

^bP < 0.01, vs NS (t test)

Table 9 Effect of BR-CH₂Cl₂ extract on proliferation of granuloma caused by cotton pellet in mice

Groups	Dose (mg/kg)	Mouse (n)	Weight of granuloma		Inhibitory rate (%)
Groups	Dose (mg/kg)	Mouse (n)	Wet w. (mg)	Dry w. (mg)	Wet w. (mg)	Dry w. (mg)
NS (A)		10	613.4 ± 160.4	130.3 ± 42.0
BR (C)	250	10	445.8 ± 37.0^b	97.9 ± 12.8^a	27.3	24.9
BR (D)	500	10	422.8 ± 33.2^b	84.8 ± 7.8^a	31.1	34.9

^aP < 0.05;

^bP < 0.01, vs NS (t test)

Citation: Yin ZZ, Jin HL, Yin XZ, Li TZ, Quan JS, Jin ZN. Effect of Boschniakia rossica on expression of GST-P, p53 and p21^ras proteins in early stage of chemical hepatocarcinogenesis and its anti-inflammatory activities in rats. World J Gastroenterol 2000; 6(6): 812-818
URL: https://www.wjgnet.com/1007-9327/full/v6/i6/812.htm
DOI: https://dx.doi.org/10.3748/wjg.v6.i6.812