Primary porcine hepatocytes with portal vein serum cultured on microcarriers or in spheroidal aggregates

Table 1 Ingredients of different liver-perfusing solutions (g/L)

	NaCl	KCl	NaOH	CaCl2	MgCl2	Na2SO4	HEPES	Collagen ase (IV)	Tricine	Tes	pH
I	8.3	0.5	0.22				2.4				7.4
II	3.9	0.5	2.6	0.53			24	0.5			7.4-7.6
III	4.0	0.4	2.1	0.136	0.13	0.1	7.2		6.5	6.9	7.4
IV	8.3	0.5	0.11	0.136			2.4				7.4

Table 2 Amounts of hepatocytes at various time in different culture systems on microcarriers (^-x±s, × 10⁵/pore)

	1st	2nd	3rd	4th	5th	6th	7th (day)
FCSs	5.0 ± 0.4	5.0 ± 0.1	6.9 ± 0.2	9.8 ± 0.6	19.3 ± 0.2	34.3 ± 0.3	58.6 ± 0.7
PPVSs	5.0 ± 1.2	5.0 ± 0.4	11.4 ± 0.5a	24.3 ± 0.1a	40.4 ± 0.4a	70.7 ± 0.7a	136.1 ± 0.1a

^aP < 0.05 (compared with FCSs group)