Susceptibility to hepatocellular carcinoma associated with null genotypes of GSTM1 and GSTT1

Table 1 The sequence of primers for PCR

Gene	Location	Sequence	Length of amplificates (bp)
GSTM1	5′	5′-CTCCTGATTATGACAGAAGCC-3′	650
	3′	5′-CTGGATTGTAGCAGATCATGC-3′
GSTT1	5′	5′-TTCCTTACTGGTCCTCACATCTC-3′	480
	3′	5′-TCACCGGATCATGGCCAGCA-3′
β-globulin	5′	5′-CAACTTCATCCACGTTCACC-3′	268
	3′	5′-GAAGAGCCAAGGACAGGTAC-3′

Table 2 The association of GSTM1 null genotype and HCC

Genotypes	Cases		Controls		Total
	n	%	n	%
Null	36	57.1	37	42.0	73
Non-null	27	42.9	51	58.0	78
Total	63	100.0	88	100.0	151

χ² = 3.35, P = 0.067, OR = 1.84 (95%CI = 0.91-3.73)

Table 3 The association of GSTT1 null genotype and HCC

Genotype	Cases		Controls		Total
	n	%	n	%
Null genotype	55	87.3	55	62.5	110
Non-null genotype	8	12.7	33	37.5	41
Total	63	100.0	88	100.0	151

χ² = 11.42, P = 0.0007274; OR = 4.13 (95%CI = 1.64-10.70)

Table 4 The relationship between different exposure levels of GSTM1, GSTT1 and HCC

Exposure level		Cases		Control		OR
GSTM1	GSTT1	n	%	n	%
-	-	1	1.6	16	18.2	1.00
+	-	7	11.1	17	19.3	6.59
-	+	26	41.3	35	39.8	11.89
+	+	29	46.0	20	22.7	23.20
Total		63	100.0	88	100.0

χ²_trend = 16.413, P = 0.000051. GSTM1 (+): GSTM1 null genotype, (-): non null genotype. GSTT1 (+): GSTT1 non null genotype, (-): null genotype.