Copyright
©The Author(s) 2000.
World J Gastroenterol. Apr 15, 2000; 6(2): 202-209
Published online Apr 15, 2000. doi: 10.3748/wjg.v6.i2.202
Published online Apr 15, 2000. doi: 10.3748/wjg.v6.i2.202
Table 1 Major sugar specificities of lectins used in this study
| Lectin | Abbreviation | Carbohydrate specificity |
| Arachis hypogaea | PNA | β-D-Gal (1-3)GalNAc |
| Bandeirae simplicifolia | BS-I | α-Gal, α-GalNAc |
| Concanavalin A | Con A | α-D-Man, α-D-Glc |
| Datura stramonium | DSA | (D-GlcNAc)2 |
| Glycine max | SBA | D-GalNAc |
| Helix promatia | HPA | D-GalNAc |
| Lens culinaris | LcH | α-D-Man |
| Narcissus pseudonarcissus | NPA | α-D-Man |
| Pisum sativum | PEA | α-D-Man |
| Tetragonolobus purpureas | Lotus A | α-L-Fuc |
| Triticum vulgaris | WGA | (D-GlcNAc)2, NeuNAc |
| Vicia faba | VFA | D-Man, D-Glc |
| Vicia sativa | VSA | D-Glc, D-Man |
| Vicia villosa | VVA | D-GalNAc |
| Vigna radiata | MBA | α-Gal |
Table 2 Lectin agglutination patterns of H. pylori coccoids and spirals
| Lectin | RH 54 | NCTC 11637 | V2 | |||
| Coccoids | Spirals | Coccoids | Spirals | Coccoids | Spirals | |
| Con A | 2 | 1 | 3 | 2 | 2 | 1 |
| LcH | 2 | 0 | 2 | 0 | 1 | 0 |
| NPA | 1 | 0 | 1 | 0 | 1 | 0 |
| PEA | 2 | 1 | 2 | W | 1 | 1 |
| VFA | 1 | 1 | 2 | 1 | 1 | 1 |
| Lotus A | 3 | 3 | 1 | 0 | 3 | 3 |
| DSA | 1 | 2 | 1 | 0 | 1 | 2 |
| WGA | 2 | 3 | 0 | 0 | 2 | 3 |
| HPA | 1 | 2 | 1 | 1 | 1 | 1 |
| SBA | 1 | 1 | 1 | 1 | 0 | 3 |
| VVA | 1 | 1 | 0 | 1 | 0 | 1 |
| VSA | 1 | 1 | 2 | 1 | 2 | 1 |
| PNA | 1 | 1 | 1 | 0 | 1 | 2 |
| BS-I | 2 | 2 | 1 | 0 | 1 | 2 |
| MBA | 1 | 3 | 1 | 1 | 0 | 1 |
| Sugar specificity/Lectins | ||||||
| Man- | Con A = Concanavalin A; | |||||
| NPA = Narcissus pseudonarcissus; | ||||||
| PEA = Pisum sativum; | ||||||
| VFA = Vicia faba | ||||||
| Fuc- | Lotus A = Tetragonolobus purpureas; | |||||
| (GlcNAc)2- | DSA = Datura stramonium; | |||||
| WGA = Triticum vulgaris | ||||||
| GalNAc- | HPA = Helix promatia; | |||||
| SBA = Glycine max; | ||||||
| VVA = Vicia villosa. | ||||||
| Glc- | VSA = Vicia sativa | |||||
| Glc- | PNA = Arachis hypogaea; | |||||
| BS-I = Bandeirae simplicifolia | ||||||
| MBA = Vigna radiata | ||||||
Table 3 Strains of H. pylori agglutinated by lectins of various specificities
| Sugar-specificity/Lectin | No. of H. pylori strains agglutinated | |
| Coccoids | Spirals | |
| α-D-Man, α-D-Glc/Con A | 3 | 3 |
| α-D-Man/LcH | 3 | 0 |
| α-D-Man/NPA | 3 | 0 |
| α-D-Man/PEA | 3 | 3 |
| D-Man, D-Glc/VFA | 3 | 3 |
| α-L-Fuc/Lotus A | 3 | 2 |
| (D-GlcNAc)2/DSA | 3 | 2 |
| (D-GlcNAc)2/WGA | 2 | 2 |
| D-GalNAc/HPA | 3 | 3 |
| D-GalNAc/SBA | 2 | 3 |
| D-GalNAc/VVA | 1 | 3 |
| D-Glc, D-Man/VSA | 3 | 3 |
| β-D-Gal (1-3)GalNAc/PNA | 3 | 2 |
| α-Gal, α-GalNAc/BS-I | 3 | 2 |
| α-Gal/MBA | 2 | 3 |
Table 4 H. pylori lectin-binding inhibition
| Inhibitor | RH 54 coccoids | RH 54 spirals | ||||
| Con A | Lotus A | WGA | Con A | Lotus A | WGA | |
| Glycoproteins | ||||||
| Bovine submaxillary mucin | 1 | 3 | 2 | 0 | 3 | 3 |
| Porcine stomach mucin | 1 | 2 | 0 | 1 | 3 | 0 |
| Fetuin | 2 | 3 | W | W | 3 | 3 |
| Asialofetuin | 1 | 3 | 1 | W | 3 | 3 |
| Bovine orosomucoid | 1 | 3 | W | 1 | 3 | 2 |
| Gangliosides II | 1 | 3 | W | 0 | 3 | 2 |
| Carbohydrates | ||||||
| Gal | 1 | 3 | 1 | W | 3 | 3 |
| GalNAc | 1 | 3 | 1 | W | 3 | 3 |
| GlcNAc | W | 3 | 1 | 0 | 3 | 3 |
| NANA | 1 | 2 | 1 | 1 | 3 | 3 |
| Man | 0 | 3 | 1 | 0 | 3 | 3 |
| Glc | W | 3 | 1 | 0 | 3 | 3 |
| Malt | 0 | 3 | 1 | 0 | 3 | 3 |
| Suc | W | 3 | 1 | 0 | 3 | 3 |
| Fuc | 1 | 0 | 2 | 0 | 0 | 3 |
| Control | 2 | 3 | 2 | 1 | 3 | 3 |
Table 5 Effect of chemical and enzymatic treatments on H. pylori lectin binding
| Inhibitor | RH 54 coccoids | RH 54 spirals | ||||
| Con A | Lotus A | WGA | Con A | Lotus A | WGA | |
| NAIO4 (0.075 M) | W | 0 | 0 | W | 0 | 0 |
| H2SO4 (0.025 N) | 1 | 3 | 2 | 1 | 3 | 3 |
| Formalin (1.0%) | 2 | 3 | 2 | 1 | 3 | 3 |
| Glycine HCl buffer (pH 2.2) | 1 | 3 | 2 | 1 | 3 | 3 |
| 60 °C (1 h) | 3 | 3 | 3 | 3 | 3 | 3 |
| Neuraminidase | 1 | 3 | 2 | 1 | 3 | 3 |
| Trypsin | 1 | 3 | 2 | 1 | 3 | 3 |
| Pepsin | 1 | 3 | 2 | 1 | 3 | 3 |
| Proteinase K | 1 | 3 | 2 | 1 | 3 | 3 |
| Chymotrypsin | 1 | 3 | 2 | 1 | 3 | 3 |
| Control | 2 | 3 | 2 | 1 | 3 | 3 |
- Citation: Khin MM, Hua JS, Ng HC, Wadström T, Ho B. Agglutination of Helicobacter pylori coccoids by lectins. World J Gastroenterol 2000; 6(2): 202-209
- URL: https://www.wjgnet.com/1007-9327/full/v6/i2/202.htm
- DOI: https://dx.doi.org/10.3748/wjg.v6.i2.202