Activation of killer cells with soluble gastric cancer antigen combined with anti-CD3 McAb

Table 1 Comparison of anti-tumor activity among the three kinds of killer cells during culture

Group	Incubation day
	3	7	10	12	15	17	20
TAK	30.3	42.8	58.7	54.0	49.9	47.0	36.9
CD3AK	25.4	39.7	47.5	50.2	38.9	38.4	27.0
LAK	23.5	34.7	38.5	30.8	14.5	10.4	8.9

Table 2 Analysis of surface markers on the killer cells (n = 6)

Incubation	CD3	CD4	CD8	CD4/CD8	NK	CD19
0 d	52.80	49.40	26.40	1.87	23.30	7.20
10 d	49.88	44.36	62.80	0.76	4.53	0.20

Table 3 Influence on the killer cells with various stimulation

Group	Stimulated by	Duplication of proliferation	Killing activity
1	IL-2 alone	2.1	38.5%
2	IL-2 alone after costimulation	3.8	40.1%
	with anti-CD3 mAb for 48 h
3	Coexistence of anti-CD3 mAb	5.3	58.7%
	and IL-2 during incubation