Transient expression and antigenic characterization of HBsAg of HBV nt551 A to G mutant

Table 1 Detection of the reactivity of the expressed HBsAg to anti-α mAb by radioimmunoassay*

Anti-αmAb	pSHBs(133Met)	pSHBsT(133Val)
A6	1118(5.82)	774(3.93)
A11	932(4.80)	744(3.76)
S17	945(4.87)	630(3.14)

*Counter per minute (cpm), the number in the parentheses is P/N-value. According to the solid RIA kit producer’s instruc-tions, P/N = (sample cmp-background)/(negative control cpm-background). Untrans fected cells were used as negative control, average cpm was 240. Blank polystyrene beads were used as back-ground, average cpm 58. P/N ≥ 2.10 is cons id ered to be positive reactivity. The more the P/N value, the stronger the r eactivity.