Transduction of human hepatocellular carcinoma cells with human γ-interferon gene via retroviral vector

Table 1 Secretion of IFN-γ and expression of HLA antigens by transduced hepatocellular carcinoma cells in culture

Cell lines	IFN-γ Production	Control*	HLA class I * *	HLA class II△
	(U/5 × 105 cells/24 h)	MCN (% positive)△△	MCN (% positive)	MCN (% positive)
QGY7701	0	18 (6)	159 (99)	26 (11)
QGY7701.3A	75	21 (5)	306 (100)	28 (77)
SMMC7721	0	9 (2)	200 (100)	13 (12)
SMMC7721.2C	150	21 (3)	348 (100)	14 (58)
BEL7704	0	20 (7)	34 (93)	21 (8)
BEL7404.6A	75	22 (5)	117 (100)	29 (47)
HHCL	0	10 (3)	232 (100)	12 (10)
HHCL.2C	100	22 (6)	340 (100)	37 (56)
HHCL.2D	25	14 (6)	440 (100)	12 (68)
HHCL.5C	100	11 (4)	401 (100)	19 (83)
HHCL.6C	75	15 (3)	550 (100)	10 (44)
HHCL.5D	200	10 (3)	341 (100)	10 (40)

^* Cells were stained with fluorescence isothiocyanatic-conjugated goat anti-mouse IgG (FITC-IgG) as a control. ^{* *} Cells were stained with anti-HLA class I McAb following with FITC-IgG. ^△Cells were stained with anti-HLA class II McAb following with FITC-IgG. ^△△Mean fluorescence channel number (MCN) and percentages of positive cells (in parenthese).

Table 2 Formation of tumors by parental and transduced hepatocellular carcinoma cells in nude mice

Cell	Incidence	Volume (cm3)	Weight (g)
QGY7701	8/8	5.58 ± 0.90	1.62 ± 0.36
QGY7701.3A	8/8	5.16 ± 16	1.60 ± 0.72
SMMC7721	7/7	3.87 ± 0.24	0.55 ± 0.12S
MMC7721.2C	5/6	1.26 ± 0.32a	0.28 ± 0.08a
BEL7404	8/8	4.32 ± 0.28	0.78 ± 0.14
BEL7404.6A	8/8	3.35 ± 0.21	0.50 ± 0.12
HHCL	8/8	2.04 ± 0.32	0.35 ± 0.18
HHCL.5D	1/7	0.15b	0.02b

All the values are measured at 6 weks after injection with equal amounts of cells (3 × 10⁶ each), and presented as the average value ± the standard error of the mean

^aP < 0.05, compared with SMMC7721 value by Student’s t test

^bP < 0.01, compared with HHCL value by Student’s t test