Identification of region-specific signaling modules governing Crohn’s pathogenesis via mass spectrometry-based integrative acetyl-proteomics

Table 1 Key enriched terms in the three colonic regions under 2,4,6-trinitrobenzenesulfonic acid-induced colitis

Category	M-colon	P-colon	D-colon	Shared
Domain	IPR000219 DH domain	IPR023780 chromo domain; IPR000330 SNF2-related	IPR023780 chromo domain; IPR000330 SNF2-related	IPR009072 histone-fold
GO-CC	GO: 0005815 microtubule organizing center	GO: 0005667 transcription factor complex	GO: 0005815 microtubule organizing center	GO: 0005694 chromosome; GO: 0000786 nucleosome
GO-MF	GO: 0005089 Rho GEF activity	GO: 0003712 transcription cofactor activity; GO: 0019787 ubiquitin-like transferase	GO: 0008270 zinc ion binding	GO: 0003677 DNA binding
GO-BP	GO: 0060070 Wnt signaling; GO: 0032434 proteasomal regulation	GO: 0051568 histone H3-K4 methylation	GO: 0032496 response to lipopolysaccharide	GO: 0043933 macromolecular complex organization; GO: 0006325 chromatin organization; GO: 0051276 chromosome organization
KEGG	map05322 SLE; map05034 alcoholism	map05203 viral carcinogenesis; map04917 prolactin signaling	map05203 viral carcinogenesis

Enrichment analysis was performed using Fisher’s exact test. Terms with a Fisher’s exact test P < 0.05 were considered significantly enriched. The listed terms represent the top enriched entries for each category [domain, Gene Ontology (GO)-cellular component, GO-molecular function, GO-biological process, Kyoto Encyclopedia of Genes and Genomes] in the proximal, mid, and distal colon. P-colon: Proximal-colon; M-colon: Mid-colon; D-colon: Distal-colon; GO: Gene Ontology; CC: Cellular component; MF: Molecular function; BP: Biological process; KEGG: Kyoto Encyclopedia of Genes and Genomes; DH: Dbl homology; GEF: Guanine nucleotide exchange factor; SLE: Systemic lupus erythematosus; SNF2: Sucrose non-fermenting 2.