Establishment of a Sprague-Dawley rat model of hepatolithiasis based on common bile duct catheterization

doi:10.3748/wjg.122778

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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Copyright: ©Author(s) 2026. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution-NonCommercial (CC BY-NC 4.0) license. No commercial re-use. See permissions. Published by Baishideng Publishing Group Inc.

World J Gastroenterol. Dec 28, 2026; 32(48): 122778
Published online Dec 28, 2026. doi: 10.3748/wjg.122778

Table 1 Reagents used during establishment of the Sprague-Dawley rat model

Reagent	Manufacturer
Lipopolysaccharide	Sigma-Aldrich, United States
Penicillin potassium for injection	Jiangxi Keda Animal Pharmaceutical Co., Ltd.
Isoflurane	Shenzhen RWD Life Science Co., Ltd.
Phosphate-buffered saline	Wuhan Servicebio Technology Co., Ltd.
4% tissue/cell fixative	Beijing Solarbio Science & Technology Co., Ltd.
Environmentally friendly dewaxing/clearing solution	Baisha Bio
Absolute ethanol	Guangdong Guanghua Sci-Tech Co., Ltd.
Liquid Blocker Super PAP Pen (immunohistochemistry pen)	Shanghai Beyotime Biotechnology Co., Ltd.
Hematoxylin staining solution	Baisha Bio
Neutral balsam (long-lasting mounting medium for microscopy)	Shanghai Beyotime Biotechnology Co., Ltd.
Silicone tubing	Jiangsu Juzhuo New Materials Co., Ltd.
Metal guidewire	Asahi Intecc Co., Ltd., Japan
0.9% saline	Kunming Nanjing Pharmaceutical Co., Ltd.
5% glucose saline	Kunming Nanjing Pharmaceutical Co., Ltd.
5-0 Mersilk (Musi) suture	Johnson & Johnson (China) Medical Devices Co., Ltd.
Triangular needle	Hangzhou Aipu Medical Devices Co., Ltd.
Microsurgical instruments	Shanghai Medical Instruments (Group) Co., Ltd.
100-µL microsyringe	Shanghai Gaoge Trading Co., Ltd.
Disposable sterile syringes	Shandong Weigao Group Medical Polymer Products Co., Ltd.

Full Size Table

Table 2 Instruments used during establishment of the Sprague-Dawley rat model

Instruments	Manufacturer
Paraffin microtome	Thermo Fisher Scientific, United States
Water bath	Shanghai Yiheng
Inverted microscope	Thermo Fisher Scientific, United States
KW-10 small/Large animal ventilator	Nanjing Calvin Biotechnology Co., Ltd.
Floor-standing high-speed refrigerated centrifuge	Himac
Multipurpose refrigerated centrifuge	Beckman X-15R
Ultrapure water system	Heal Force Bio-meditech Holdings Limited, China
Ice maker	Sanyo, Japan
Drying oven	Shanghai Yiheng
-80 °C freezer	Thermo Fisher Scientific, United States
-20 °C freezer	Haier, China
Fourier transform infrared spectrometer, NICOLET iS20	Thermo Fisher Scientific, United States

Full Size Table

Citation: Shi JL, Muhammad T, He TT, Lin ZY, Zhang TG, Liu SY, Yang XL, Yang Y, Hu L, Ran P, Numan M, Li J. Establishment of a Sprague-Dawley rat model of hepatolithiasis based on common bile duct catheterization. World J Gastroenterol 2026; 32(48): 122778
URL: https://www.wjgnet.com/1007-9327/full/v32/i48/122778.htm
DOI: https://dx.doi.org/10.3748/wjg.122778

Shi JL, Muhammad T, He TT, Lin ZY, Zhang TG, Liu SY, Yang XL, Yang Y, Hu L, Ran P, Numan M, Li J. Establishment of a Sprague-Dawley rat model of hepatolithiasis based on common bile duct catheterization. World J Gastroenterol 2026; 32(48): 122778 [DOI: 10.3748/wjg.122778]

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