Autophagy related RHEB-CSF1R complex promotes tumor metastasis via advancing phosphorylation levels of PI3K, AKT, mTOR in pancreatic cancer

Table 1 Sequences of the transferred vectors

	Sequences
Negative control	UUCUCCGAACGUGUCACGU(dT)(dT)
	ACGUGACACGUUCGGAGAA(dT)(dT)
RHEB/siRNA	RHEB-siRNA1: GTGTATTCTGTTACATCAA
	RHEB-siRNA2: CACAGTAAATGGACAAGAA
	RHEB-siRNA3: GAAAGACCTGCATATGGAA
RHEB/shRNA	RHEB-shRNA1: CCGGCCTCAGACATACTCCATAGATCTCGAGATCTATGGAGTATGTCTGAGGTTTTTT
	RHEB-shRNA2: CCGGTTATGTTGGTTGGGAATAAGACTCGAGTCTTATTCCCAACCAACATAATTTTTT
	RHEB-shRNA3: CCGGCCTACGATCCAACCATAGAAACTCGAGTTTCTATGGTTGGATCGTAGGTTTTTT
	RHEB-shRNA4: CCGGTATCATCTTCAACTTGTAGACCTCGAGGTCTACAAGTTGAAGATGATATTTTTT
CSF1R/shRNA	CSF1R-shRNA1: CTGCTGACTGTTGAGACCTTA
	CSF1R-shRNA2: CGACTATAAGAACATCCACCT
	CSF1R-shRNA3: CCAACAACGCTACCTTCCAAA
	CSF1R-shRNA4: ACAGGAGAGAGCGGGACTATA

RHEB: Ras homolog enriched in brain; siRNA: Small interfering RNA; shRNA: Short hairpin RNA; CSF1R: Colony stimulating factor 1 receptor.

Table 2 List of primers used reverse transcription-polymerase chain reaction and reverse transcription-quantitative polymerase chain reaction

Primer	Sequence (5′-3′)	Molecular weight (kDa)	Annealing temperature (°C)
CSF1R-F	ATCTTCTCACTTGGGCTGAATCC	6965.46	60
CSF1R-R	AGATTGGTATAGTCCCGCTCTCT	7005.46
FGF22-F	GTGGTCATCAAAGCAGTGTCCT	6750.27	60
FGF22-R	GTAGGTGTTGTGGCCGTTCT	6185.83
AMTN-F	AGCAGGAGGAGCAGGTGTAAAT	6897.3	60
AMTN-R	ACCAAATTCGAGGCAGCTTAGTT	7047.51
TNFSF12-TNFSF13-F	ATAGCCAGGTCCTGTTTCAAGAC	7023.48	60
TNFSF12-TNFSF13-R	AAATGGAAGACACCTGCGCTATA	7065.63
UQCRHL-F	GGAGGAAGAGGAAGAGGAGGAAT	7324.5	60
UQCRHL-R	CGTGCAACCTCTTCTGTATGTG	6996.45
DACT1-F	AGTGACCTGAGACTGGATGTAGA	7152.49	60
DACT1-R	GGTGCTGGAATGACAACATGGATA	7152.49
ARRDC4-F	ACTGCCATTAGTGATCGGTACAA	7047.51	60
ARRDC-R	CACACTTCTCCCTCACAGTTAGG	6919.45
GAPDH-F	CACTCCTCCACCTTTGACGC	5948.9	60
GAPDH-R	CTGTTGCTGTAGCCAAATTCGT	6716.4

CSF1R: Colony stimulating factor 1 receptor; GAPDH: Glyceraldehyde 3-phosphate dehydrogenase.