Runchangningshen paste activates NLRP6 inflammasome-mediated autophagy to stimulate colonic mucin-2 secretion and modulates mucosal microbiota in functional constipation

doi:10.3748/wjg.v31.i9.102256

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Mar 7, 2025 (publication date) through Nov 11, 2025

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World Journal of Gastroenterology

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1007-9327

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World J Gastroenterol. Mar 7, 2025; 31(9): 102256
Published online Mar 7, 2025. doi: 10.3748/wjg.v31.i9.102256

Table 1 The primer information

Gene	Sequence (5‘-3’)
β-actin	Forward: GGCTGTGTTGTCCCTGTATGC
β-actin	Reverse: TCACGCACGATTTCCCTCTC
NLRP6	Forward: AAGGGATGAAGCAGTGTCT
NLRP6	Reverse: GGAGGTATTGGCGGTTAT
Caspase1	Forward: CTGGGAAGAGGTAGAAAC
Caspase1	Reverse: TTTTGAAGATGATGGCA
ASC	Forward: GGACCCCATAGACCTCACTG
ASC	Reverse: CCATACAGAGCATCCAGCAA
IL18	Forward: CACTTTGGCAGACTTCA
IL18	Reverse: GTCCTCTTACTTCACTATCTT
IL1β	Forward: GACAGTGAGGAGAATGACCT
IL1β	Reverse: GGTGCTTGGGTCCTCAT
Muc2	Forward: TCGTAGTAGTGCTTGGGAGGC
Muc2	Reverse: TGCTGCTGATGAGTGGTTGG

IL: Interleukin; Muc2: Mucin-2.

Full Size Table

Citation: Liu XJ, Ye-er-tai YLY, Jia YB, Wu CH, Wang XX, Yang KM, Yao X, Ling JH. Runchangningshen paste activates NLRP6 inflammasome-mediated autophagy to stimulate colonic mucin-2 secretion and modulates mucosal microbiota in functional constipation. World J Gastroenterol 2025; 31(9): 102256
URL: https://www.wjgnet.com/1007-9327/full/v31/i9/102256.htm
DOI: https://dx.doi.org/10.3748/wjg.v31.i9.102256